RLG00000013112

Endochitinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000495
Physical Location & Seq
Forward (+)
26314240 .. 26315661
1422 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013112

Sequence Viewer

Length: 990 bp
ATGCTGTACTTGGCCGTGTCATTTGTTTCTCTTCTATTGTTCTTTAATATCCGAGGAGGCTCAGCAGAGCAATGTGGAAGGCAAGCAGGGGGGGCTTCGTGCCCGGGAGGGCAGTGCTGCAGCAAGTTTGGCTGGTGTGGCACTACACCTGATTACTGCGGCACCGGTTGCCAAAGCCAATGTGGCGGTGGTGGTGGCGGGGGCGGTGGTGACATTGGGGGTCTAGTATCAAAGGCCACCTTTAATCAGATACTTAAGCATCGGAACGATGGTGGTTGCCCTGCCAAAGGGTTTTATACTTATGATGCTTTCATTGCAGCTGCCAAGGCTTTCCCCGCCTTTGCTACAACTGGTGACACTGCCACCCGTAAAAGAGAGATTGCTGCTTTCTTAGCTCAAACTTCTCATGAAACTACTGGGGGATGGGGGACTGCACCTGATGGACCCTATGCATGGGGCTATTGTTTTAACAAGGAGAAAAATCCAGGATCTTACTGTGCCTCGGATCCAAATTATCCATGTGCTGCTGGTAAGCAGTATTATGGCCGTGGTCCTATTCAACTTTCTTGGAACTACAACTACGGGCAGTGTGGAAAGGCCATAGGAGCGAACCTGTTGAACGACCCGGACCTTGTTGCCACAGACCCTGTCATCTCCTTCAAGACAGCAATCTGGTTCTGGATGACCCCTCAGTCACCCAAGCCCTCATGCCATGATGTCATCACCTGGAGATGGAACCCTTCCGGAGCTGATCAGTCAGCGGGTAGGTATTCCGGGTATGGTACGATCACAAATATCATCAACGGTGGACTCGAATGTGGTAAAGGATGGGATGGGAGAGTGGCGGACCGCATTGGGTTTTTCAAGAGGTACTGTGATTTGCTTGGGATTGGTTATGGTGACAACCTTGACTGTTATAATCAGAAACCTTTTGGGTCTGGACTCAGTACTTATTATTCATCCTCAAGAGAGTCGATCGATGTAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00520 ko01100 ko04016 map00520 map01100 map04016
Pfam Domains
Protein Families
Chitin_bind_1 Glyco_hydro_19

Protein Analysis

330

Amino Acids

34.98

Weight (kDa)

8.05

Isoelectric Point (pI)

37.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chitin_bind_1 PF00187 24 - 61 4.7e-14 Chitin recognition protein
Glyco_hydro_19 PF00182 75 - 305 3e-127 Chitinase class I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000495)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12500
fragaria_vesca FvH4_1g10640 FvH4_1g10640 FvH4_1g10640 FvH4_1g10640 FvH4_1g10640 FvH4_1g10641 FvH4_1g10641 FvH4_1g10642 FvH4_1g10643 FvH4_1g10650 FvH4_1g10660 FvH4_1g10660 FvH4_1g10661 FvH4_2g16190
malus_domestica MD02G1120200.v1.1 MD02G1120300.v1.1 MD15G1234400.v1.1
prunus_persica Prupe.7G178500_v2.0.a1 Prupe.8G174900_v2.0.a1
pyrus_communis pycom02g09370 pycom02g09380 pycom02g09390
rosa_chinensis RchiOBHm_Chr2g0097671 RchiOBHm_Chr2g0097691 RchiOBHm_Chr2g0097731 RchiOBHm_Chr2g0097781 RchiOBHm_Chr2g0097801 RchiOBHm_Chr2g0097821 RchiOBHm_Chr6g0279811 RchiOBHm_Chr6g0279831 RchiOBHm_Chr6g0279841
rosa_laevigata RLG00000013112 RLG00000013114 RLG00000016734 RLG00000016736 RLG00000016737 RLG00000016738
rosa_multiflora Rmu_sc0000974.1_g000004 Rmu_sc0000974.1_g000013 Rmu_sc0000974.1_g000019 Rmu_sc0000974.1_g000025 Rmu_sc0002340.1_g000010 Rmu_sc0008442.1_g000003 Rmu_sc0008442.1_g000009 Rmu_sc0020401.1_g000002
rosa_roxburghii Rroxscaffold_2G00140520 Rroxscaffold_2G00144540 Rroxscaffold_2G00144580 Rroxscaffold_2G00144590 Rroxscaffold_7G00188570 Rroxscaffold_7G00188590 Rroxscaffold_7G00188610
rosa_rugosa Rorug02G0068300 Rorug02G0068400 Rorug06G0128600
rosa_samantha Rh2AG114800 Rh2AG114900 Rh2AG115000 Rh2AG115100 Rh2BG117600 Rh2BG117800 Rh2BG117900 Rh2BG118000 Rh2CG119600 Rh2DG118500 Rh2DG118800 Rh2DG119100 Rh2DG119200 Rh2DG119300 Rh5AG369400 Rh5CG403900 Rh6AG236600 Rh6AG236700 Rh6AG236800 Rh6AG237300 Rh6BG241000 Rh6BG241200 Rh6BG241400 Rh6CG243300 Rh6CG243400 Rh6CG243600 Rh6DG234400 Rh6DG234500 Rh7AG282100
rosa_wichuraiana Rw2G008980 Rw2G008990 Rw6G020640

Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 918
AasI GACNNNNNNGTC 1 cut(s) 691
AccB1I GGYRCC 1 cut(s) 161
AccIII TCCGGA 1 cut(s) 743
AciI CCGC 8 cut(s) 159, 186, 198, 204, 336, 761, 845, 850
AclWI GGATC 3 cut(s) 496, 500, 513
AcoI YGGCCR 2 cut(s) 12, 544
AfaI GTAC 4 cut(s) 8, 784, 872, 949
AfiI CCNNNNNNNGG 3 cut(s) 287, 453, 732
AflII CTTAAG 1 cut(s) 254
AgeI ACCGGT 1 cut(s) 164
AgsI TTSAA 4 cut(s) 560, 619, 661, 865
AjnI CCWGG 2 cut(s) 484, 725
AluBI AGCT 3 cut(s) 320, 395, 749
AluI AGCT 3 cut(s) 320, 395, 749
AlwI GGATC 3 cut(s) 496, 500, 513
AlwNI CAGNNNCTG 1 cut(s) 647
Ama87I CYCGRG 1 cut(s) 103
Aor13HI TCCGGA 1 cut(s) 743
AoxI GGCC 4 cut(s) 12, 234, 544, 597
ApeKI GCWGC 6 cut(s) 117, 120, 317, 320, 383, 524
AsiGI ACCGGT 1 cut(s) 164
AspS9I GGNCC 4 cut(s) 443, 551, 628, 847
AsuC2I CCSGG 4 cut(s) 104, 105, 626, 775
AsuHPI GGTGA 5 cut(s) 221, 365, 687, 715, 911
AvaI CYCGRG 1 cut(s) 103
AvaII GGWCC 4 cut(s) 443, 551, 628, 847
BaeGI GKGCMC 1 cut(s) 104
BamHI GGATCC 1 cut(s) 505
BanI GGYRCC 1 cut(s) 161
BbvI GCAGC 6 cut(s) 104, 132, 307, 329, 370, 511
BccI CCATC 6 cut(s) 263, 417, 434, 726, 822, 827
BceAI ACGGC 1 cut(s) 531
BciT130I CCWGG 2 cut(s) 486, 727
BclI TGATCA 1 cut(s) 751
BcnI CCSGG 4 cut(s) 104, 105, 626, 775
BfaI CTAG 1 cut(s) 224
BfmI CTRYAG 1 cut(s) 118
BfrI CTTAAG 1 cut(s) 254
BglI GCCNNNNNGGC 1 cut(s) 183
BisI GCNGC 7 cut(s) 118, 121, 160, 318, 321, 384, 525
BlpI GCTNAGC 1 cut(s) 61
BlsI GCNGC 7 cut(s) 119, 122, 161, 319, 322, 385, 526
BmcAI AGTACT 1 cut(s) 949
Bme1390I CCNGG 6 cut(s) 104, 105, 486, 626, 727, 775
Bme18I GGWCC 4 cut(s) 443, 551, 628, 847
BmeT110I CYCGRG 1 cut(s) 103
BmgT120I GGNCC 4 cut(s) 443, 551, 628, 847
BmiI GGNNCC 4 cut(s) 163, 445, 507, 737
BmrFI CCNGG 6 cut(s) 104, 105, 486, 626, 727, 775
BmrI ACTGGG 1 cut(s) 426
BmsI GCATC 2 cut(s) 268, 295
BmuI ACTGGG 1 cut(s) 426
BpmI CTGGAG 1 cut(s) 748
Bpu1102I GCTNAGC 1 cut(s) 61
BpuEI CTTGAG 1 cut(s) 949
BpuMI CCSGG 4 cut(s) 104, 105, 626, 775
Bsa29I ATCGAT 1 cut(s) 978
BsaJI CCNNGG 5 cut(s) 52, 103, 324, 501, 547
BsaWI WCCGGW 2 cut(s) 164, 743
Bsc4I CCNNNNNNNGG 3 cut(s) 287, 453, 732
Bse118I RCCGGY 1 cut(s) 164
Bse1I ACTGG 2 cut(s) 355, 421
Bse3DI GCAATG 2 cut(s) 77, 312
BseAI TCCGGA 1 cut(s) 743
BseBI CCWGG 2 cut(s) 486, 727
BseCI ATCGAT 1 cut(s) 978
BseDI CCNNGG 5 cut(s) 52, 103, 324, 501, 547
BseGI GGATG 5 cut(s) 428, 687, 833, 838, 959
BseLI CCNNNNNNNGG 3 cut(s) 287, 453, 732
BseMI GCAATG 2 cut(s) 77, 312
BseMII CTCAG 3 cut(s) 75, 704, 958
BseNI ACTGG 2 cut(s) 355, 421
BseRI GAGGAG 1 cut(s) 69
BseSI GKGCMC 1 cut(s) 104
BseXI GCAGC 6 cut(s) 104, 132, 307, 329, 370, 511
BsgI GTGCAG 1 cut(s) 417
Bsh1285I CGRYCG 1 cut(s) 978
BshFI GGCC 4 cut(s) 14, 236, 546, 599
BshNI GGYRCC 1 cut(s) 161
BshTI ACCGGT 1 cut(s) 164
BshVI ATCGAT 1 cut(s) 978
BsiEI CGRYCG 1 cut(s) 978
BsiHKCI CYCGRG 1 cut(s) 103
BsiSI CCGG 5 cut(s) 104, 165, 626, 744, 774
BslFI GGGAC 1 cut(s) 442
BslI CCNNNNNNNGG 3 cut(s) 287, 453, 732
BsmFI GGGAC 1 cut(s) 442
BsnI GGCC 4 cut(s) 14, 236, 546, 599
BsoBI CYCGRG 1 cut(s) 103
Bsp1286I GDGCHC 1 cut(s) 104
Bsp13I TCCGGA 1 cut(s) 743
Bsp143I GATC 5 cut(s) 488, 505, 751, 786, 975
Bsp1720I GCTNAGC 1 cut(s) 61
BspACI CCGC 8 cut(s) 159, 186, 198, 204, 336, 761, 845, 850
BspANI GGCC 4 cut(s) 14, 236, 546, 599
BspCNI CTCAG 3 cut(s) 74, 703, 957
BspDI ATCGAT 1 cut(s) 978
BspEI TCCGGA 1 cut(s) 743
BspHI TCATGA 1 cut(s) 406
BspLI GGNNCC 4 cut(s) 163, 445, 507, 737
BspMAI CTGCAG 1 cut(s) 122
BspPI GGATC 3 cut(s) 496, 500, 513
BspT107I GGYRCC 1 cut(s) 161
BspTI CTTAAG 1 cut(s) 254
BsrDI GCAATG 2 cut(s) 77, 312
BsrFI RCCGGY 1 cut(s) 164
BsrI ACTGG 2 cut(s) 355, 421
BssAI RCCGGY 1 cut(s) 164
BssECI CCNNGG 5 cut(s) 52, 103, 324, 501, 547
BssMI GATC 5 cut(s) 488, 505, 751, 786, 975
BssT1I CCWWGG 1 cut(s) 324
Bst2UI CCWGG 2 cut(s) 486, 727
Bst4CI ACNGT 4 cut(s) 497, 806, 875, 914
Bst6I CTCTTC 1 cut(s) 36
BstAFI CTTAAG 1 cut(s) 254
BstAPI GCANNNNNTGC 1 cut(s) 168
BstC8I GCNNGC 1 cut(s) 84
BstDEI CTNAG 4 cut(s) 61, 391, 690, 944
BstDSI CCRYGG 1 cut(s) 547
BstENI CCTNNNNNAGG 1 cut(s) 285
BstF5I GGATG 5 cut(s) 428, 687, 833, 838, 959
BstKTI GATC 5 cut(s) 491, 508, 754, 789, 978
BstMBI GATC 5 cut(s) 488, 505, 751, 786, 975
BstMCI CGRYCG 1 cut(s) 978
BstNI CCWGG 2 cut(s) 486, 727
BstSCI CCNGG 6 cut(s) 102, 103, 484, 624, 725, 773
BstSFI CTRYAG 1 cut(s) 118
BstSLI GKGCMC 1 cut(s) 104
BstV1I GCAGC 6 cut(s) 104, 132, 307, 329, 370, 511
BstX2I RGATCY 2 cut(s) 488, 505
BstYI RGATCY 2 cut(s) 488, 505
Bsu15I ATCGAT 1 cut(s) 978
BsuRI GGCC 4 cut(s) 14, 236, 546, 599
BsuTUI ATCGAT 1 cut(s) 978
BtgI CCRYGG 1 cut(s) 547
BtsCI GGATG 5 cut(s) 428, 687, 833, 838, 959
BtsI GCAGTG 3 cut(s) 119, 357, 593
BtsIMutI CAGTG 3 cut(s) 119, 357, 593
Cac8I GCNNGC 1 cut(s) 84
CaiI CAGNNNCTG 1 cut(s) 647
CciI TCATGA 1 cut(s) 406
Cfr10I RCCGGY 1 cut(s) 164
Cfr13I GGNCC 4 cut(s) 443, 551, 628, 847
Cfr9I CCCGGG 1 cut(s) 103
ClaI ATCGAT 1 cut(s) 978
CpoI CGGWCCG 1 cut(s) 847
Csp6I GTAC 4 cut(s) 7, 783, 871, 948
CspAI ACCGGT 1 cut(s) 164
CspI CGGWCCG 1 cut(s) 847
CviAII CATG 5 cut(s) 407, 453, 519, 708, 713
CviQI GTAC 4 cut(s) 7, 783, 871, 948
DdeI CTNAG 4 cut(s) 61, 391, 690, 944
DpnI GATC 5 cut(s) 490, 507, 753, 788, 977
DpnII GATC 5 cut(s) 488, 505, 751, 786, 975
DrdI GACNNNNNNGTC 1 cut(s) 691
DseDI GACNNNNNNGTC 1 cut(s) 691
EaeI YGGCCR 2 cut(s) 12, 544
Eam1104I CTCTTC 1 cut(s) 36
EarI CTCTTC 1 cut(s) 36
EciI GGCGGA 1 cut(s) 860
Eco130I CCWWGG 1 cut(s) 324
Eco47I GGWCC 4 cut(s) 443, 551, 628, 847
Eco88I CYCGRG 1 cut(s) 103
EcoNI CCTNNNNNAGG 1 cut(s) 285
EcoRII CCWGG 2 cut(s) 484, 725
EcoT14I CCWWGG 1 cut(s) 324
EcoT22I ATGCAT 1 cut(s) 454
ErhI CCWWGG 1 cut(s) 324
FaeI CATG 5 cut(s) 410, 456, 522, 711, 716
FalI AAGNNNNNCTT 2 cut(s) 224, 256
FaqI GGGAC 1 cut(s) 442
FatI CATG 5 cut(s) 406, 452, 518, 707, 712
FauI CCCGC 3 cut(s) 191, 343, 754
FbaI TGATCA 1 cut(s) 751
Fnu4HI GCNGC 7 cut(s) 118, 121, 160, 318, 321, 384, 525
FokI GGATG 5 cut(s) 435, 694, 840, 845, 946
Fsp4HI GCNGC 7 cut(s) 118, 121, 160, 318, 321, 384, 525
FspBI CTAG 1 cut(s) 224
GluI GCNGC 7 cut(s) 118, 121, 160, 318, 321, 384, 525
GsuI CTGGAG 1 cut(s) 748
HaeIII GGCC 4 cut(s) 14, 236, 546, 599
HapII CCGG 5 cut(s) 104, 165, 626, 744, 774
Hin1II CATG 5 cut(s) 410, 456, 522, 711, 716
HinfI GANTC 3 cut(s) 810, 942, 971
HpaII CCGG 5 cut(s) 104, 165, 626, 744, 774
HphI GGTGA 5 cut(s) 221, 365, 687, 715, 911
Hpy166II GTNNAC 1 cut(s) 809
Hpy188I TCNGA 5 cut(s) 53, 249, 264, 505, 924
Hpy188III TCNNGA 7 cut(s) 407, 661, 679, 744, 865, 939, 966
Hpy8I GTNNAC 1 cut(s) 809
HpyAV CCTTC 3 cut(s) 72, 667, 750
HpyCH4III ACNGT 4 cut(s) 497, 806, 875, 914
HpyCH4V TGCA 4 cut(s) 120, 317, 434, 452
HpyF3I CTNAG 4 cut(s) 61, 391, 690, 944
Hsp92II CATG 5 cut(s) 410, 456, 522, 711, 716
Kpn2I TCCGGA 1 cut(s) 743
Ksp22I TGATCA 1 cut(s) 751
Kzo9I GATC 5 cut(s) 488, 505, 751, 786, 975
LmnI GCTCC 2 cut(s) 605, 746
Lsp1109I GCAGC 6 cut(s) 104, 132, 307, 329, 370, 511
LweI GCATC 2 cut(s) 268, 295
MaeI CTAG 1 cut(s) 224
MaeIII GTNAC 4 cut(s) 209, 353, 693, 899
MalI GATC 5 cut(s) 490, 507, 753, 788, 977
MboI GATC 5 cut(s) 488, 505, 751, 786, 975
MboII GAAGA 1 cut(s) 23
MflI RGATCY 2 cut(s) 488, 505
MhlI GDGCHC 1 cut(s) 104
MluCI AATT 1 cut(s) 511
MlyI GAGTC 3 cut(s) 804, 936, 980
MnlI CCTC 8 cut(s) 47, 50, 101, 511, 699, 715, 861, 973
Mph1103I ATGCAT 1 cut(s) 454
MroI TCCGGA 1 cut(s) 743
MseI TTAA 4 cut(s) 45, 243, 255, 468
MspA1I CMGCKG 2 cut(s) 320, 761
MspCI CTTAAG 1 cut(s) 254
MspI CCGG 5 cut(s) 104, 165, 626, 744, 774
MspR9I CCNGG 6 cut(s) 104, 105, 486, 626, 727, 775
MvaI CCWGG 2 cut(s) 486, 727
NciI CCSGG 4 cut(s) 104, 105, 626, 775
NdeII GATC 5 cut(s) 488, 505, 751, 786, 975
NlaIII CATG 5 cut(s) 410, 456, 522, 711, 716
NlaIV GGNNCC 4 cut(s) 163, 445, 507, 737
NmuCI GTSAC 4 cut(s) 209, 353, 693, 899
NsiI ATGCAT 1 cut(s) 454
PagI TCATGA 1 cut(s) 406
PcsI WCGNNNNNNNCGW 1 cut(s) 810
PflFI GACNNNGTC 1 cut(s) 647
PfoI TCCNGGA 1 cut(s) 484
PinAI ACCGGT 1 cut(s) 164
PkrI GCNGC 7 cut(s) 119, 122, 161, 319, 322, 385, 526
Ple19I CGATCG 1 cut(s) 978
PleI GAGTC 3 cut(s) 804, 936, 979
PpsI GAGTC 3 cut(s) 804, 936, 979
PsiI TTATAA 1 cut(s) 918
Psp6I CCWGG 2 cut(s) 484, 725
PspGI CCWGG 2 cut(s) 484, 725
PspN4I GGNNCC 4 cut(s) 163, 445, 507, 737
PspPI GGNCC 4 cut(s) 443, 551, 628, 847
PsrI GAACNNNNNNTAC 2 cut(s) 563, 595
PstI CTGCAG 1 cut(s) 122
PstNI CAGNNNCTG 1 cut(s) 647
PsuI RGATCY 2 cut(s) 488, 505
PsyI GACNNNGTC 1 cut(s) 647
PvuI CGATCG 1 cut(s) 978
PvuII CAGCTG 1 cut(s) 320
RsaI GTAC 4 cut(s) 8, 784, 872, 949
RsaNI GTAC 4 cut(s) 7, 783, 871, 948
Rsr2I CGGWCCG 1 cut(s) 847
RsrII CGGWCCG 1 cut(s) 847
SaqAI TTAA 4 cut(s) 45, 243, 255, 468
SatI GCNGC 7 cut(s) 118, 121, 160, 318, 321, 384, 525
Sau3AI GATC 5 cut(s) 488, 505, 751, 786, 975
Sau96I GGNCC 4 cut(s) 443, 551, 628, 847
ScaI AGTACT 1 cut(s) 949
SchI GAGTC 3 cut(s) 804, 936, 980
ScrFI CCNGG 6 cut(s) 104, 105, 486, 626, 727, 775
SduI GDGCHC 1 cut(s) 104
SfaNI GCATC 2 cut(s) 268, 295
SfcI CTRYAG 1 cut(s) 118
SinI GGWCC 4 cut(s) 443, 551, 628, 847
SmaI CCCGGG 1 cut(s) 105
SmlI CTYRAG 2 cut(s) 254, 964
SmoI CTYRAG 2 cut(s) 254, 964
Sse9I AATT 1 cut(s) 511
SsiI CCGC 8 cut(s) 159, 186, 198, 204, 336, 761, 845, 850
SspMI CTAG 1 cut(s) 224
StyD4I CCNGG 6 cut(s) 102, 103, 484, 624, 725, 773
StyI CCWWGG 1 cut(s) 324
TaaI ACNGT 4 cut(s) 497, 806, 875, 914
TaqI TCGA 3 cut(s) 813, 974, 978
TasI AATT 1 cut(s) 511
TatI WGTACW 2 cut(s) 6, 947
TauI GCSGC 1 cut(s) 162
Tru1I TTAA 4 cut(s) 45, 243, 255, 468
Tru9I TTAA 4 cut(s) 45, 243, 255, 468
TscAI CASTG 3 cut(s) 119, 364, 593
TseFI GTSAC 4 cut(s) 209, 353, 693, 899
TseI GCWGC 6 cut(s) 117, 120, 317, 320, 383, 524
Tsp45I GTSAC 4 cut(s) 209, 353, 693, 899
TspDTI ATGAA 3 cut(s) 301, 423, 948
TspMI CCCGGG 1 cut(s) 103
TspRI CASTG 3 cut(s) 119, 364, 593
Tth111I GACNNNGTC 1 cut(s) 647
Vha464I CTTAAG 1 cut(s) 254
VpaK11BI GGWCC 4 cut(s) 443, 551, 628, 847
XagI CCTNNNNNAGG 1 cut(s) 285
XcmI CCANNNNNNNNNTGG 2 cut(s) 179, 185
XmaI CCCGGG 1 cut(s) 103
XspI CTAG 1 cut(s) 224
ZrmI AGTACT 1 cut(s) 949
Zsp2I ATGCAT 1 cut(s) 454
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.