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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AfiI | CCNNNNNNNGG | 2 cut(s) | 21, 70 |
| AoxI | GGCC | 1 cut(s) | 56 |
| AspS9I | GGNCC | 1 cut(s) | 99 |
| AvaII | GGWCC | 1 cut(s) | 99 |
| BbsI | GAAGAC | 1 cut(s) | 155 |
| BfaI | CTAG | 1 cut(s) | 111 |
| BglII | AGATCT | 1 cut(s) | 113 |
| Bme18I | GGWCC | 1 cut(s) | 99 |
| BmgT120I | GGNCC | 1 cut(s) | 99 |
| BpiI | GAAGAC | 1 cut(s) | 155 |
| BsaJI | CCNNGG | 1 cut(s) | 30 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 21, 70 |
| BseDI | CCNNGG | 1 cut(s) | 30 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 21, 70 |
| BseRI | GAGGAG | 2 cut(s) | 153, 156 |
| BshFI | GGCC | 1 cut(s) | 58 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 21, 70 |
| BsnI | GGCC | 1 cut(s) | 58 |
| Bsp143I | GATC | 2 cut(s) | 85, 113 |
| BspANI | GGCC | 1 cut(s) | 58 |
| BssECI | CCNNGG | 1 cut(s) | 30 |
| BssMI | GATC | 2 cut(s) | 85, 113 |
| BssT1I | CCWWGG | 1 cut(s) | 30 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 26 |
| BstKTI | GATC | 2 cut(s) | 88, 116 |
| BstMBI | GATC | 2 cut(s) | 85, 113 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 26 |
| BstV2I | GAAGAC | 1 cut(s) | 155 |
| BstX2I | RGATCY | 1 cut(s) | 113 |
| BstYI | RGATCY | 1 cut(s) | 113 |
| BsuRI | GGCC | 1 cut(s) | 58 |
| Cfr13I | GGNCC | 1 cut(s) | 99 |
| CviJI | RGCY | 1 cut(s) | 58 |
| CviKI_1 | RGCY | 1 cut(s) | 58 |
| DpnI | GATC | 2 cut(s) | 87, 115 |
| DpnII | GATC | 2 cut(s) | 85, 113 |
| Eco130I | CCWWGG | 1 cut(s) | 30 |
| Eco47I | GGWCC | 1 cut(s) | 99 |
| EcoT14I | CCWWGG | 1 cut(s) | 30 |
| ErhI | CCWWGG | 1 cut(s) | 30 |
| FaiI | YATR | 1 cut(s) | 17 |
| FspBI | CTAG | 1 cut(s) | 111 |
| HaeIII | GGCC | 1 cut(s) | 58 |
| HinfI | GANTC | 1 cut(s) | 129 |
| Hpy188I | TCNGA | 1 cut(s) | 65 |
| HpyAV | CCTTC | 1 cut(s) | 90 |
| HpyCH4IV | ACGT | 1 cut(s) | 37 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 26 |
| HpySE526I | ACGT | 1 cut(s) | 37 |
| Kzo9I | GATC | 2 cut(s) | 85, 113 |
| MaeI | CTAG | 1 cut(s) | 111 |
| MaeII | ACGT | 1 cut(s) | 37 |
| MalI | GATC | 2 cut(s) | 87, 115 |
| MboI | GATC | 2 cut(s) | 85, 113 |
| MboII | GAAGA | 2 cut(s) | 105, 160 |
| MflI | RGATCY | 1 cut(s) | 113 |
| MmeI | TCCRAC | 1 cut(s) | 88 |
| MnlI | CCTC | 3 cut(s) | 48, 131, 134 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 26 |
| NdeII | GATC | 2 cut(s) | 85, 113 |
| PfeI | GAWTC | 1 cut(s) | 129 |
| PspPI | GGNCC | 1 cut(s) | 99 |
| PsuI | RGATCY | 1 cut(s) | 113 |
| Sau3AI | GATC | 2 cut(s) | 85, 113 |
| Sau96I | GGNCC | 1 cut(s) | 99 |
| SetI | ASST | 1 cut(s) | 40 |
| SgeI | CNNG | 8 cut(s) | 33, 43, 48, 82, 101, 123, 129, 137 |
| SinI | GGWCC | 1 cut(s) | 99 |
| SspMI | CTAG | 1 cut(s) | 111 |
| StyI | CCWWGG | 1 cut(s) | 30 |
| TaiI | ACGT | 1 cut(s) | 40 |
| TaqI | TCGA | 1 cut(s) | 132 |
| TaqII | GACCGA | 1 cut(s) | 116 |
| TfiI | GAWTC | 1 cut(s) | 129 |
| VpaK11BI | GGWCC | 1 cut(s) | 99 |
| XspI | CTAG | 1 cut(s) | 111 |