Rroxscaffold_2G00144580

Endochitinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000495
Physical Location & Seq
Reverse (-)
82463576 .. 82465132
1557 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00144580.1

Sequence Viewer

Length: 699 bp
ATGAAGTTCCACACTCTTATGAGTCTTATCAGCCTCTCCCTAACTTTGGTGATAGAGGCCTTGGCGAGTAGTGTGGGAGACAATCCGGAAGTTCAACGTGTCCAAATGGGCTGTGTTGTAGCCAATATGGGTGGTGTGGCACAACAGCCGATTCGTGGAACCGGCTACCGAAGCCAATGTAGTTACGGGGGTGGCGGGGATGGCTCAACCCCGACCCCAAGTGGTGGTGATGATGTTAGTAGTATAATTAGCGCATCTTTTTTTGATCAAATGCTTAAGTACCGAAATGATGGTCGGTGCCCAAGTAATGGGTTTTATAAGTATGATGCTTTCATTGCTGCCCGATCCTTTAATGGGTTTGGCACAACCGGAGATGTTGCTACACTCAAGAAGGCCGGAACTTGCTTTGCTTTCCTGGGCATTTCAAGTTTTGATTGGGAGAATGAGGGCAATATTGCAAGAAATATTTTGGAGGAAATCATATGGCACGAGGATACGAAAGTTGCGAGGGTATACTATGCCTTAATCTTCCATACCTACCTCTTCATTTCAGCATCAATTCCATCTCTCCCCAATCTATTCATCTCCTCATCCTCTTTCAAAGCACCACGACAACCCACCACCCCTAAACCTCGGAGGGTTAGCTACACCGGTTGGATGGTGCGAAATCACAATGACGACACGGTTCCGTGTGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00520 ko01100 ko04016 map00520 map01100 map04016
Pfam Domains
Protein Families
Chitin_bind_1 Glyco_hydro_19

Protein Analysis

232

Amino Acids

25.31

Weight (kDa)

7.64

Isoelectric Point (pI)

42.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_19 PF00182 82 - 145 1.2e-16 Chitinase class I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000495)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12500
fragaria_vesca FvH4_1g10640 FvH4_1g10640 FvH4_1g10640 FvH4_1g10640 FvH4_1g10640 FvH4_1g10641 FvH4_1g10641 FvH4_1g10642 FvH4_1g10643 FvH4_1g10650 FvH4_1g10660 FvH4_1g10660 FvH4_1g10661 FvH4_2g16190
malus_domestica MD02G1120200.v1.1 MD02G1120300.v1.1 MD15G1234400.v1.1
prunus_persica Prupe.7G178500_v2.0.a1 Prupe.8G174900_v2.0.a1
pyrus_communis pycom02g09370 pycom02g09380 pycom02g09390
rosa_chinensis RchiOBHm_Chr2g0097671 RchiOBHm_Chr2g0097691 RchiOBHm_Chr2g0097731 RchiOBHm_Chr2g0097781 RchiOBHm_Chr2g0097801 RchiOBHm_Chr2g0097821 RchiOBHm_Chr6g0279811 RchiOBHm_Chr6g0279831 RchiOBHm_Chr6g0279841
rosa_laevigata RLG00000013112 RLG00000013114 RLG00000016734 RLG00000016736 RLG00000016737 RLG00000016738
rosa_multiflora Rmu_sc0000974.1_g000004 Rmu_sc0000974.1_g000013 Rmu_sc0000974.1_g000019 Rmu_sc0000974.1_g000025 Rmu_sc0002340.1_g000010 Rmu_sc0008442.1_g000003 Rmu_sc0008442.1_g000009 Rmu_sc0020401.1_g000002
rosa_roxburghii Rroxscaffold_2G00140520 Rroxscaffold_2G00144540 Rroxscaffold_2G00144580 Rroxscaffold_2G00144590 Rroxscaffold_7G00188570 Rroxscaffold_7G00188590 Rroxscaffold_7G00188610
rosa_rugosa Rorug02G0068300 Rorug02G0068400 Rorug06G0128600
rosa_samantha Rh2AG114800 Rh2AG114900 Rh2AG115000 Rh2AG115100 Rh2BG117600 Rh2BG117800 Rh2BG117900 Rh2BG118000 Rh2CG119600 Rh2DG118500 Rh2DG118800 Rh2DG119100 Rh2DG119200 Rh2DG119300 Rh5AG369400 Rh5CG403900 Rh6AG236600 Rh6AG236700 Rh6AG236800 Rh6AG237300 Rh6BG241000 Rh6BG241200 Rh6BG241400 Rh6CG243300 Rh6CG243400 Rh6CG243600 Rh6DG234400 Rh6DG234500 Rh7AG282100
rosa_wichuraiana Rw2G008980 Rw2G008990 Rw6G020640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 318
AccB1I GGYRCC 1 cut(s) 297
AccB7I CCANNNNNTGG 2 cut(s) 224, 308
AccI GTMKAC 1 cut(s) 513
AccIII TCCGGA 1 cut(s) 85
AciI CCGC 1 cut(s) 195
AclWI GGATC 1 cut(s) 339
AfaI GTAC 1 cut(s) 281
AfiI CCNNNNNNNGG 5 cut(s) 46, 155, 224, 308, 354
AflII CTTAAG 1 cut(s) 275
AflIII ACRYGT 1 cut(s) 97
AgeI ACCGGT 1 cut(s) 650
AgsI TTSAA 3 cut(s) 95, 426, 601
AjnI CCWGG 1 cut(s) 414
AluBI AGCT 1 cut(s) 645
AluI AGCT 1 cut(s) 645
Alw26I GTCTC 1 cut(s) 72
AlwI GGATC 1 cut(s) 339
Aor13HI TCCGGA 1 cut(s) 85
AoxI GGCC 2 cut(s) 57, 393
ApeKI GCWGC 1 cut(s) 338
ArsI GACNNNNNNTTYG 2 cut(s) 277, 309
AsiGI ACCGGT 1 cut(s) 650
AspLEI GCGC 1 cut(s) 254
AsuHPI GGTGA 2 cut(s) 61, 239
BaeGI GKGCMC 1 cut(s) 302
BaeI ACNNNNGTAYC 2 cut(s) 486, 519
BanI GGYRCC 1 cut(s) 297
BauI CACGAG 1 cut(s) 488
BbvI GCAGC 1 cut(s) 325
BccI CCATC 4 cut(s) 194, 284, 571, 652
BciT130I CCWGG 1 cut(s) 416
BciVI GTATCC 1 cut(s) 487
BclI TGATCA 1 cut(s) 265
BcoDI GTCTC 1 cut(s) 72
BfrI CTTAAG 1 cut(s) 275
BfuI GTATCC 1 cut(s) 487
BisI GCNGC 1 cut(s) 339
BlsI GCNGC 1 cut(s) 340
Bme1390I CCNGG 1 cut(s) 416
BmiI GGNNCC 3 cut(s) 160, 299, 687
BmrFI CCNGG 1 cut(s) 416
BmsI GCATC 3 cut(s) 263, 316, 563
BpuEI CTTGAG 1 cut(s) 371
BsaJI CCNNGG 3 cut(s) 60, 415, 632
BsaWI WCCGGW 3 cut(s) 85, 368, 650
Bsc4I CCNNNNNNNGG 5 cut(s) 46, 155, 224, 308, 354
Bse118I RCCGGY 2 cut(s) 161, 650
Bse3DI GCAATG 1 cut(s) 333
BseAI TCCGGA 1 cut(s) 85
BseBI CCWGG 1 cut(s) 416
BseDI CCNNGG 3 cut(s) 60, 415, 632
BseGI GGATG 3 cut(s) 205, 590, 663
BseLI CCNNNNNNNGG 5 cut(s) 46, 155, 224, 308, 354
BseMI GCAATG 1 cut(s) 333
BseRI GAGGAG 1 cut(s) 577
BseSI GKGCMC 1 cut(s) 302
BseXI GCAGC 1 cut(s) 325
BshFI GGCC 2 cut(s) 59, 395
BshNI GGYRCC 1 cut(s) 297
BshTI ACCGGT 1 cut(s) 650
BsiSI CCGG 5 cut(s) 86, 162, 369, 396, 651
BslI CCNNNNNNNGG 5 cut(s) 46, 155, 224, 308, 354
BsmAI GTCTC 1 cut(s) 72
BsnI GGCC 2 cut(s) 59, 395
Bsp1286I GDGCHC 1 cut(s) 302
Bsp13I TCCGGA 1 cut(s) 85
Bsp143I GATC 2 cut(s) 265, 344
BspACI CCGC 1 cut(s) 195
BspANI GGCC 2 cut(s) 59, 395
BspEI TCCGGA 1 cut(s) 85
BspLI GGNNCC 3 cut(s) 160, 299, 687
BspPI GGATC 1 cut(s) 339
BspT107I GGYRCC 1 cut(s) 297
BspTI CTTAAG 1 cut(s) 275
BsrDI GCAATG 1 cut(s) 333
BsrFI RCCGGY 2 cut(s) 161, 650
BssAI RCCGGY 2 cut(s) 161, 650
BssECI CCNNGG 3 cut(s) 60, 415, 632
BssMI GATC 2 cut(s) 265, 344
BssNAI GTATAC 1 cut(s) 514
BssSI CACGAG 1 cut(s) 488
BssT1I CCWWGG 1 cut(s) 60
Bst1107I GTATAC 1 cut(s) 514
Bst2BI CACGAG 1 cut(s) 488
Bst2UI CCWGG 1 cut(s) 416
Bst4CI ACNGT 1 cut(s) 685
Bst6I CTCTTC 1 cut(s) 548
BstAFI CTTAAG 1 cut(s) 275
BstF5I GGATG 3 cut(s) 205, 590, 663
BstHHI GCGC 1 cut(s) 254
BstKTI GATC 2 cut(s) 268, 347
BstMAI GTCTC 1 cut(s) 72
BstMBI GATC 2 cut(s) 265, 344
BstMWI GCNNNNNNNGC 3 cut(s) 171, 201, 335
BstNI CCWGG 1 cut(s) 416
BstSCI CCNGG 1 cut(s) 414
BstSLI GKGCMC 1 cut(s) 302
BstV1I GCAGC 1 cut(s) 325
BstZ17I GTATAC 1 cut(s) 514
BsuI GTATCC 1 cut(s) 487
BsuRI GGCC 2 cut(s) 59, 395
BtsCI GGATG 3 cut(s) 205, 590, 663
CfoI GCGC 1 cut(s) 254
Cfr10I RCCGGY 2 cut(s) 161, 650
Csp6I GTAC 1 cut(s) 280
CspAI ACCGGT 1 cut(s) 650
CspCI CAANNNNNGTGG 2 cut(s) 112, 147
CviQI GTAC 1 cut(s) 280
DpnI GATC 2 cut(s) 267, 346
DpnII GATC 2 cut(s) 265, 344
Eam1104I CTCTTC 1 cut(s) 548
EarI CTCTTC 1 cut(s) 548
Eco130I CCWWGG 1 cut(s) 60
Eco147I AGGCCT 1 cut(s) 59
EcoRII CCWGG 1 cut(s) 414
EcoT14I CCWWGG 1 cut(s) 60
ErhI CCWWGG 1 cut(s) 60
FauI CCCGC 1 cut(s) 188
FauNDI CATATG 1 cut(s) 482
FbaI TGATCA 1 cut(s) 265
FblI GTMKAC 1 cut(s) 513
Fnu4HI GCNGC 1 cut(s) 339
FokI GGATG 3 cut(s) 212, 577, 670
Fsp4HI GCNGC 1 cut(s) 339
GlaI GCGC 1 cut(s) 253
GluI GCNGC 1 cut(s) 339
HaeIII GGCC 2 cut(s) 59, 395
HapII CCGG 5 cut(s) 86, 162, 369, 396, 651
HhaI GCGC 1 cut(s) 254
Hin6I GCGC 1 cut(s) 252
HinP1I GCGC 1 cut(s) 252
HinfI GANTC 2 cut(s) 22, 151
HpaII CCGG 5 cut(s) 86, 162, 369, 396, 651
HphI GGTGA 2 cut(s) 61, 239
Hpy166II GTNNAC 1 cut(s) 514
Hpy188I TCNGA 1 cut(s) 636
Hpy188III TCNNGA 2 cut(s) 86, 388
Hpy8I GTNNAC 1 cut(s) 514
HpyAV CCTTC 1 cut(s) 385
HpyCH4III ACNGT 1 cut(s) 685
HpyCH4IV ACGT 1 cut(s) 97
HpyCH4V TGCA 1 cut(s) 458
HpyF10VI GCNNNNNNNGC 3 cut(s) 171, 201, 335
HpySE526I ACGT 1 cut(s) 97
HspAI GCGC 1 cut(s) 252
Kpn2I TCCGGA 1 cut(s) 85
Ksp22I TGATCA 1 cut(s) 265
Kzo9I GATC 2 cut(s) 265, 344
LpnPI CCDG 7 cut(s) 99, 175, 382, 401, 409, 428, 664
Lsp1109I GCAGC 1 cut(s) 325
LweI GCATC 3 cut(s) 263, 316, 563
MaeII ACGT 1 cut(s) 97
MaeIII GTNAC 1 cut(s) 182
MalI GATC 2 cut(s) 267, 346
MboI GATC 2 cut(s) 265, 344
MboII GAAGA 2 cut(s) 520, 535
MhlI GDGCHC 1 cut(s) 302
MluCI AATT 2 cut(s) 246, 558
MlyI GAGTC 1 cut(s) 31
MmeI TCCRAC 1 cut(s) 635
MroI TCCGGA 1 cut(s) 85
MseI TTAA 3 cut(s) 276, 351, 524
MslI CAYNNNNRTG 1 cut(s) 17
MspCI CTTAAG 1 cut(s) 275
MspI CCGG 5 cut(s) 86, 162, 369, 396, 651
MspR9I CCNGG 1 cut(s) 416
MvaI CCWGG 1 cut(s) 416
MwoI GCNNNNNNNGC 3 cut(s) 171, 201, 335
NdeI CATATG 1 cut(s) 482
NdeII GATC 2 cut(s) 265, 344
NlaIV GGNNCC 3 cut(s) 160, 299, 687
PceI AGGCCT 1 cut(s) 59
PcsI WCGNNNNNNNCGW 1 cut(s) 503
PfeI GAWTC 1 cut(s) 151
PflMI CCANNNNNTGG 2 cut(s) 224, 308
PinAI ACCGGT 1 cut(s) 650
PkrI GCNGC 1 cut(s) 340
PleI GAGTC 1 cut(s) 30
PpsI GAGTC 1 cut(s) 30
PsiI TTATAA 1 cut(s) 318
Psp6I CCWGG 1 cut(s) 414
PspGI CCWGG 1 cut(s) 414
PspN4I GGNNCC 3 cut(s) 160, 299, 687
RsaI GTAC 1 cut(s) 281
RsaNI GTAC 1 cut(s) 280
RseI CAYNNNNRTG 1 cut(s) 17
SaqAI TTAA 3 cut(s) 276, 351, 524
SatI GCNGC 1 cut(s) 339
Sau3AI GATC 2 cut(s) 265, 344
SchI GAGTC 1 cut(s) 31
ScrFI CCNGG 1 cut(s) 416
SduI GDGCHC 1 cut(s) 302
SetI ASST 5 cut(s) 100, 539, 543, 634, 647
SfaNI GCATC 3 cut(s) 263, 316, 563
SmiMI CAYNNNNRTG 1 cut(s) 17
SmlI CTYRAG 2 cut(s) 275, 386
SmoI CTYRAG 2 cut(s) 275, 386
Sse9I AATT 2 cut(s) 246, 558
SseBI AGGCCT 1 cut(s) 59
SsiI CCGC 1 cut(s) 195
SspI AATATT 2 cut(s) 454, 466
StuI AGGCCT 1 cut(s) 59
StyD4I CCNGG 1 cut(s) 414
StyI CCWWGG 1 cut(s) 60
TaaI ACNGT 1 cut(s) 685
TaiI ACGT 1 cut(s) 100
TasI AATT 2 cut(s) 246, 558
TfiI GAWTC 1 cut(s) 151
Tru1I TTAA 3 cut(s) 276, 351, 524
Tru9I TTAA 3 cut(s) 276, 351, 524
TseI GCWGC 1 cut(s) 338
TspDTI ATGAA 4 cut(s) 17, 322, 535, 571
TspGWI ACGGA 1 cut(s) 678
Van91I CCANNNNNTGG 2 cut(s) 224, 308
Vha464I CTTAAG 1 cut(s) 275
XmiI GTMKAC 1 cut(s) 513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.