Rroxscaffold_2G00144590

Endochitinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000495
Physical Location & Seq
Reverse (-)
82473245 .. 82474410
1166 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00144590.1

Sequence Viewer

Length: 609 bp
ATGCTGAAATATCGAAACGATGGGCGCTGCCCTAGCAATGGGTTCTACAAGTATGACGCTTTCATTTCTGCTGCTCGATCTTTCAATGGGTTTGGTACAACAGGCGATGTGGCTACCCAGAAAAAAGAGCTTGCTGCTTTCTTGGGTCAAACCTCTCACGAAACCACTGGAGGATGGCCAAGTGCACCAGATGGTCCTTATGCTTGGGGATATTGCTTTATCAGGGAAAACAACCAAGACGTCAACTACAACTATGCTCAAGCGGGTAGAGCAATCGGAGTGGATCTGATAAACAATCCAGATCTAGTAGCCACAGACCCGGTTGTATCATTCAAGACAGCTATATGGTTTTGGATGACCCCTCAAGGAAACAAACCATCAAGTCACGACGTCATTACCGGTCGTTGGAATCCATCTGCTGCAGATAGATCGGCGGGTCGGGTCCCTGGATATGGAGTGATTACAAACATAATTAATGGCGGACTTGAATGTGGGCATGGTCAAGATGATAGGGTAGTTGATCGGATCGGGTTCTACAAGAGGTACTGTGGCATATTGGGAGTGAGCACGGGGGACAACTTGGACTGTAACAATCAAAGGCCATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00520 ko01100 ko04016 map00520 map01100 map04016
Pfam Domains
Protein Families
Chitin_bind_1 Glyco_hydro_19

Protein Analysis

202

Amino Acids

22.12

Weight (kDa)

6.81

Isoelectric Point (pI)

33.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_19 PF00182 1 - 83 2.4e-38 Chitinase class I
Glyco_hydro_19 PF00182 81 - 196 1.5e-67 Chitinase class I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000495)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12500
fragaria_vesca FvH4_1g10640 FvH4_1g10640 FvH4_1g10640 FvH4_1g10640 FvH4_1g10640 FvH4_1g10641 FvH4_1g10641 FvH4_1g10642 FvH4_1g10643 FvH4_1g10650 FvH4_1g10660 FvH4_1g10660 FvH4_1g10661 FvH4_2g16190
malus_domestica MD02G1120200.v1.1 MD02G1120300.v1.1 MD15G1234400.v1.1
prunus_persica Prupe.7G178500_v2.0.a1 Prupe.8G174900_v2.0.a1
pyrus_communis pycom02g09370 pycom02g09380 pycom02g09390
rosa_chinensis RchiOBHm_Chr2g0097671 RchiOBHm_Chr2g0097691 RchiOBHm_Chr2g0097731 RchiOBHm_Chr2g0097781 RchiOBHm_Chr2g0097801 RchiOBHm_Chr2g0097821 RchiOBHm_Chr6g0279811 RchiOBHm_Chr6g0279831 RchiOBHm_Chr6g0279841
rosa_laevigata RLG00000013112 RLG00000013114 RLG00000016734 RLG00000016736 RLG00000016737 RLG00000016738
rosa_multiflora Rmu_sc0000974.1_g000004 Rmu_sc0000974.1_g000013 Rmu_sc0000974.1_g000019 Rmu_sc0000974.1_g000025 Rmu_sc0002340.1_g000010 Rmu_sc0008442.1_g000003 Rmu_sc0008442.1_g000009 Rmu_sc0020401.1_g000002
rosa_roxburghii Rroxscaffold_2G00140520 Rroxscaffold_2G00144540 Rroxscaffold_2G00144580 Rroxscaffold_2G00144590 Rroxscaffold_7G00188570 Rroxscaffold_7G00188590 Rroxscaffold_7G00188610
rosa_rugosa Rorug02G0068300 Rorug02G0068400 Rorug06G0128600
rosa_samantha Rh2AG114800 Rh2AG114900 Rh2AG115000 Rh2AG115100 Rh2BG117600 Rh2BG117800 Rh2BG117900 Rh2BG118000 Rh2CG119600 Rh2DG118500 Rh2DG118800 Rh2DG119100 Rh2DG119200 Rh2DG119300 Rh5AG369400 Rh5CG403900 Rh6AG236600 Rh6AG236700 Rh6AG236800 Rh6AG237300 Rh6BG241000 Rh6BG241200 Rh6BG241400 Rh6CG243300 Rh6CG243400 Rh6CG243600 Rh6DG234400 Rh6DG234500 Rh7AG282100
rosa_wichuraiana Rw2G008980 Rw2G008990 Rw6G020640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 243, 393
AciI CCGC 3 cut(s) 263, 434, 480
AclWI GGATC 2 cut(s) 291, 533
AcoI YGGCCR 1 cut(s) 176
AcyI GRCGYC 2 cut(s) 240, 390
AfaI GTAC 2 cut(s) 97, 545
AfiI CCNNNNNNNGG 3 cut(s) 38, 405, 452
AgeI ACCGGT 1 cut(s) 398
AgsI TTSAA 3 cut(s) 85, 334, 488
AjnI CCWGG 1 cut(s) 445
AluBI AGCT 2 cut(s) 130, 341
AluI AGCT 2 cut(s) 130, 341
Alw21I GWGCWC 2 cut(s) 187, 569
Alw44I GTGCAC 1 cut(s) 183
AlwI GGATC 2 cut(s) 291, 533
AoxI GGCC 2 cut(s) 176, 599
ApaLI GTGCAC 1 cut(s) 183
ApeKI GCWGC 4 cut(s) 27, 71, 134, 419
AseI ATTAAT 1 cut(s) 474
AsiGI ACCGGT 1 cut(s) 398
AspLEI GCGC 1 cut(s) 27
AspS9I GGNCC 2 cut(s) 194, 442
AsuC2I CCSGG 1 cut(s) 320
AvaII GGWCC 2 cut(s) 194, 442
BaeGI GKGCMC 1 cut(s) 187
BalI TGGCCA 1 cut(s) 178
Bbv12I GWGCWC 2 cut(s) 187, 569
BbvI GCAGC 4 cut(s) 14, 58, 121, 406
BccI CCATC 5 cut(s) 14, 168, 185, 385, 421
BcgI CGANNNNNNTGC 2 cut(s) 411, 445
BciT130I CCWGG 1 cut(s) 447
BcnI CCSGG 1 cut(s) 320
BfaI CTAG 2 cut(s) 33, 305
BfmI CTRYAG 1 cut(s) 420
BfoI RGCGCY 1 cut(s) 28
BglII AGATCT 1 cut(s) 301
BisI GCNGC 4 cut(s) 28, 72, 135, 420
BlsI GCNGC 4 cut(s) 29, 73, 136, 421
Bme1390I CCNGG 2 cut(s) 320, 447
Bme18I GGWCC 2 cut(s) 194, 442
BmgT120I GGNCC 2 cut(s) 194, 442
BmiI GGNNCC 2 cut(s) 443, 444
BmrFI CCNGG 2 cut(s) 320, 447
BpmI CTGGAG 1 cut(s) 189
BpuEI CTTGAG 2 cut(s) 243, 348
BpuMI CCSGG 1 cut(s) 320
BsaHI GRCGYC 2 cut(s) 240, 390
BsaJI CCNNGG 1 cut(s) 445
BsaWI WCCGGW 1 cut(s) 398
Bsc4I CCNNNNNNNGG 3 cut(s) 38, 405, 452
Bse118I RCCGGY 1 cut(s) 398
Bse1I ACTGG 1 cut(s) 172
Bse3DI GCAATG 1 cut(s) 43
BseBI CCWGG 1 cut(s) 447
BseDI CCNNGG 1 cut(s) 445
BseGI GGATG 2 cut(s) 179, 360
BseLI CCNNNNNNNGG 3 cut(s) 38, 405, 452
BseMI GCAATG 1 cut(s) 43
BseNI ACTGG 1 cut(s) 172
BseSI GKGCMC 1 cut(s) 187
BseXI GCAGC 4 cut(s) 14, 58, 121, 406
Bsh1285I CGRYCG 1 cut(s) 403
BshFI GGCC 2 cut(s) 178, 601
BshTI ACCGGT 1 cut(s) 398
BsiEI CGRYCG 1 cut(s) 403
BsiHKAI GWGCWC 2 cut(s) 187, 569
BsiSI CCGG 2 cut(s) 320, 399
BslFI GGGAC 2 cut(s) 428, 587
BslI CCNNNNNNNGG 3 cut(s) 38, 405, 452
BsmFI GGGAC 2 cut(s) 428, 587
BsnI GGCC 2 cut(s) 178, 601
Bsp1286I GDGCHC 2 cut(s) 187, 569
Bsp143I GATC 6 cut(s) 77, 283, 301, 428, 520, 525
BspACI CCGC 3 cut(s) 263, 434, 480
BspANI GGCC 2 cut(s) 178, 601
BspLI GGNNCC 2 cut(s) 443, 444
BspMAI CTGCAG 1 cut(s) 424
BspPI GGATC 2 cut(s) 291, 533
BsrDI GCAATG 1 cut(s) 43
BsrFI RCCGGY 1 cut(s) 398
BsrI ACTGG 1 cut(s) 172
BssAI RCCGGY 1 cut(s) 398
BssECI CCNNGG 1 cut(s) 445
BssMI GATC 6 cut(s) 77, 283, 301, 428, 520, 525
BssNI GRCGYC 2 cut(s) 240, 390
Bst2UI CCWGG 1 cut(s) 447
Bst4CI ACNGT 2 cut(s) 548, 587
BstACI GRCGYC 2 cut(s) 240, 390
BstC8I GCNNGC 1 cut(s) 132
BstF5I GGATG 2 cut(s) 179, 360
BstH2I RGCGCY 1 cut(s) 28
BstHHI GCGC 1 cut(s) 27
BstKTI GATC 6 cut(s) 80, 286, 304, 431, 523, 528
BstMBI GATC 6 cut(s) 77, 283, 301, 428, 520, 525
BstMCI CGRYCG 1 cut(s) 403
BstMWI GCNNNNNNNGC 2 cut(s) 33, 269
BstNI CCWGG 1 cut(s) 447
BstSCI CCNGG 2 cut(s) 318, 445
BstSFI CTRYAG 1 cut(s) 420
BstSLI GKGCMC 1 cut(s) 187
BstV1I GCAGC 4 cut(s) 14, 58, 121, 406
BstX2I RGATCY 2 cut(s) 283, 301
BstYI RGATCY 2 cut(s) 283, 301
BsuRI GGCC 2 cut(s) 178, 601
BtgZI GCGATG 1 cut(s) 120
BtsCI GGATG 2 cut(s) 179, 360
BtsIMutI CAGTG 1 cut(s) 165
Cac8I GCNNGC 1 cut(s) 132
CfoI GCGC 1 cut(s) 27
Cfr10I RCCGGY 1 cut(s) 398
Cfr13I GGNCC 2 cut(s) 194, 442
CseI GACGC 1 cut(s) 65
Csp6I GTAC 2 cut(s) 96, 544
CspAI ACCGGT 1 cut(s) 398
CspCI CAANNNNNGTGG 2 cut(s) 261, 296
CviAII CATG 1 cut(s) 497
CviJI RGCY 6 cut(s) 113, 130, 178, 311, 341, 601
CviKI_1 RGCY 6 cut(s) 113, 130, 178, 311, 341, 601
CviQI GTAC 2 cut(s) 96, 544
DpnI GATC 6 cut(s) 79, 285, 303, 430, 522, 527
DpnII GATC 6 cut(s) 77, 283, 301, 428, 520, 525
EaeI YGGCCR 1 cut(s) 176
EciI GGCGGA 1 cut(s) 495
Eco47I GGWCC 2 cut(s) 194, 442
EcoO109I RGGNCCY 1 cut(s) 442
EcoRII CCWGG 1 cut(s) 445
FaeI CATG 1 cut(s) 500
FaiI YATR 9 cut(s) 54, 201, 255, 344, 346, 453, 470, 498, 554
FaqI GGGAC 2 cut(s) 428, 587
FatI CATG 1 cut(s) 496
FauI CCCGC 2 cut(s) 256, 427
Fnu4HI GCNGC 4 cut(s) 28, 72, 135, 420
FokI GGATG 2 cut(s) 186, 367
Fsp4HI GCNGC 4 cut(s) 28, 72, 135, 420
FspBI CTAG 2 cut(s) 33, 305
GlaI GCGC 1 cut(s) 26
GluI GCNGC 4 cut(s) 28, 72, 135, 420
GsuI CTGGAG 1 cut(s) 189
HaeII RGCGCY 1 cut(s) 28
HaeIII GGCC 2 cut(s) 178, 601
HapII CCGG 2 cut(s) 320, 399
HgaI GACGC 1 cut(s) 65
HhaI GCGC 1 cut(s) 27
Hin1I GRCGYC 2 cut(s) 240, 390
Hin1II CATG 1 cut(s) 500
Hin6I GCGC 1 cut(s) 25
HinP1I GCGC 1 cut(s) 25
HincII GTYRAC 1 cut(s) 244
HindII GTYRAC 1 cut(s) 244
HinfI GANTC 1 cut(s) 409
HpaII CCGG 2 cut(s) 320, 399
Hpy166II GTNNAC 2 cut(s) 185, 244
Hpy188I TCNGA 3 cut(s) 278, 288, 525
Hpy188III TCNNGA 5 cut(s) 158, 299, 334, 386, 503
Hpy8I GTNNAC 2 cut(s) 185, 244
Hpy99I CGWCG 1 cut(s) 392
HpyCH4III ACNGT 2 cut(s) 548, 587
HpyCH4IV ACGT 2 cut(s) 240, 390
HpyCH4V TGCA 2 cut(s) 185, 422
HpyF10VI GCNNNNNNNGC 2 cut(s) 33, 269
HpySE526I ACGT 2 cut(s) 240, 390
Hsp92I GRCGYC 2 cut(s) 240, 390
Hsp92II CATG 1 cut(s) 500
HspAI GCGC 1 cut(s) 25
KflI GGGWCCC 1 cut(s) 442
Kzo9I GATC 6 cut(s) 77, 283, 301, 428, 520, 525
Lsp1109I GCAGC 4 cut(s) 14, 58, 121, 406
MaeI CTAG 2 cut(s) 33, 305
MaeII ACGT 2 cut(s) 240, 390
MaeIII GTNAC 2 cut(s) 383, 587
MalI GATC 6 cut(s) 79, 285, 303, 430, 522, 527
MboI GATC 6 cut(s) 77, 283, 301, 428, 520, 525
MflI RGATCY 2 cut(s) 283, 301
MhlI GDGCHC 2 cut(s) 187, 569
MlsI TGGCCA 1 cut(s) 178
MluCI AATT 1 cut(s) 471
MluNI TGGCCA 1 cut(s) 178
MmeI TCCRAC 1 cut(s) 386
MnlI CCTC 4 cut(s) 163, 164, 372, 534
Mox20I TGGCCA 1 cut(s) 178
MscI TGGCCA 1 cut(s) 178
MseI TTAA 1 cut(s) 474
Msp20I TGGCCA 1 cut(s) 178
MspI CCGG 2 cut(s) 320, 399
MspR9I CCNGG 2 cut(s) 320, 447
MvaI CCWGG 1 cut(s) 447
MwoI GCNNNNNNNGC 2 cut(s) 33, 269
NciI CCSGG 1 cut(s) 320
NdeII GATC 6 cut(s) 77, 283, 301, 428, 520, 525
NlaIII CATG 1 cut(s) 500
NlaIV GGNNCC 2 cut(s) 443, 444
NmuCI GTSAC 1 cut(s) 383
PfeI GAWTC 1 cut(s) 409
PinAI ACCGGT 1 cut(s) 398
PkrI GCNGC 4 cut(s) 29, 73, 136, 421
PpuMI RGGWCCY 1 cut(s) 442
PshBI ATTAAT 1 cut(s) 474
Psp5II RGGWCCY 1 cut(s) 442
Psp6I CCWGG 1 cut(s) 445
PspGI CCWGG 1 cut(s) 445
PspN4I GGNNCC 2 cut(s) 443, 444
PspPI GGNCC 2 cut(s) 194, 442
PspPPI RGGWCCY 1 cut(s) 442
PstI CTGCAG 1 cut(s) 424
PsuI RGATCY 2 cut(s) 283, 301
RsaI GTAC 2 cut(s) 97, 545
RsaNI GTAC 2 cut(s) 96, 544
SaqAI TTAA 1 cut(s) 474
SatI GCNGC 4 cut(s) 28, 72, 135, 420
Sau3AI GATC 6 cut(s) 77, 283, 301, 428, 520, 525
Sau96I GGNCC 2 cut(s) 194, 442
ScrFI CCNGG 2 cut(s) 320, 447
SduI GDGCHC 2 cut(s) 187, 569
SetI ASST 6 cut(s) 132, 155, 243, 343, 393, 545
SfcI CTRYAG 1 cut(s) 420
SinI GGWCC 2 cut(s) 194, 442
SmlI CTYRAG 2 cut(s) 258, 363
SmoI CTYRAG 2 cut(s) 258, 363
Sse9I AATT 1 cut(s) 471
SsiI CCGC 3 cut(s) 263, 434, 480
SspMI CTAG 2 cut(s) 33, 305
StyD4I CCNGG 2 cut(s) 318, 445
TaaI ACNGT 2 cut(s) 548, 587
TaiI ACGT 2 cut(s) 243, 393
TaqI TCGA 2 cut(s) 13, 76
TasI AATT 1 cut(s) 471
TfiI GAWTC 1 cut(s) 409
Tru1I TTAA 1 cut(s) 474
Tru9I TTAA 1 cut(s) 474
TscAI CASTG 1 cut(s) 172
TseFI GTSAC 1 cut(s) 383
TseI GCWGC 4 cut(s) 27, 71, 134, 419
Tsp45I GTSAC 1 cut(s) 383
TspDTI ATGAA 1 cut(s) 52
TspRI CASTG 1 cut(s) 172
VneI GTGCAC 1 cut(s) 183
VpaK11BI GGWCC 2 cut(s) 194, 442
VspI ATTAAT 1 cut(s) 474
XspI CTAG 2 cut(s) 33, 305
ZraI GACGTC 2 cut(s) 241, 391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.