Rh6DG234400

Endochitinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0000495
Physical Location & Seq
Reverse (-)
41772930 .. 41775357
2428 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG234400.1

Sequence Viewer

Length: 558 bp
ATGGCTGGTGTGGCACTACACCTGATTATTGCGGCAACGGTTGCCAAAGCCAATGCGGCCGCGGGTCTAGTATCACAGAACACCTTTGATCAGATACTTAAGCATCGAAACGATGGTGGTTGCCCTGCCAAATGGTTTTATACTTACGATGCTTTTATTGCAGCTGCCAAGGCTTTCCCCGGCTTTGCTACTACTGGTGACACTGCCACCCGTAAAAGGGAGATTGCTGCTTTCTTAGCCCAAACTTCTCATGAAACTACTGGGGGATGGGGGACTGCCCCTGATGGACCAAACTCATGGGGCTATTGTTATAACAAGGAGCAAAATCCAGGAGCTTACTGTGCCTGGGATCCAAATTACCCATGTGCTGCTGGTAAGCGGTATTATGGCCGAGGTTCTATTCAACTTTCTTGGAGCTACAACTACGGGCAGTGTGGAAAGGCCATAGGAGCGAACCTGTTGAACGACCCGGACCTTGTTGCCACAGACCCTGTCATGTCCTTCAAGACAGCAATCTGGTTCTGGATGACCTCAGTCACCCAAGCCCTCATACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00520 ko01100 ko04016 map00520 map01100 map04016
Pfam Domains
Protein Families
Chitin_bind_1 Glyco_hydro_19

Protein Analysis

185

Amino Acids

19.94

Weight (kDa)

8.24

Isoelectric Point (pI)

24.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_19 PF00182 23 - 178 1e-80 Chitinase class I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000495)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12500
fragaria_vesca FvH4_1g10640 FvH4_1g10640 FvH4_1g10640 FvH4_1g10640 FvH4_1g10640 FvH4_1g10641 FvH4_1g10641 FvH4_1g10642 FvH4_1g10643 FvH4_1g10650 FvH4_1g10660 FvH4_1g10660 FvH4_1g10661 FvH4_2g16190
malus_domestica MD02G1120200.v1.1 MD02G1120300.v1.1 MD15G1234400.v1.1
prunus_persica Prupe.7G178500_v2.0.a1 Prupe.8G174900_v2.0.a1
pyrus_communis pycom02g09370 pycom02g09380 pycom02g09390
rosa_chinensis RchiOBHm_Chr2g0097671 RchiOBHm_Chr2g0097691 RchiOBHm_Chr2g0097731 RchiOBHm_Chr2g0097781 RchiOBHm_Chr2g0097801 RchiOBHm_Chr2g0097821 RchiOBHm_Chr6g0279811 RchiOBHm_Chr6g0279831 RchiOBHm_Chr6g0279841
rosa_laevigata RLG00000013112 RLG00000013114 RLG00000016734 RLG00000016736 RLG00000016737 RLG00000016738
rosa_multiflora Rmu_sc0000974.1_g000004 Rmu_sc0000974.1_g000013 Rmu_sc0000974.1_g000019 Rmu_sc0000974.1_g000025 Rmu_sc0002340.1_g000010 Rmu_sc0008442.1_g000003 Rmu_sc0008442.1_g000009 Rmu_sc0020401.1_g000002
rosa_roxburghii Rroxscaffold_2G00140520 Rroxscaffold_2G00144540 Rroxscaffold_2G00144580 Rroxscaffold_2G00144590 Rroxscaffold_7G00188570 Rroxscaffold_7G00188590 Rroxscaffold_7G00188610
rosa_rugosa Rorug02G0068300 Rorug02G0068400 Rorug06G0128600
rosa_samantha Rh2AG114800 Rh2AG114900 Rh2AG115000 Rh2AG115100 Rh2BG117600 Rh2BG117800 Rh2BG117900 Rh2BG118000 Rh2CG119600 Rh2DG118500 Rh2DG118800 Rh2DG119100 Rh2DG119200 Rh2DG119300 Rh5AG369400 Rh5CG403900 Rh6AG236600 Rh6AG236700 Rh6AG236800 Rh6AG237300 Rh6BG241000 Rh6BG241200 Rh6BG241400 Rh6CG243300 Rh6CG243400 Rh6CG243600 Rh6DG234400 Rh6DG234500 Rh7AG282100
rosa_wichuraiana Rw2G008980 Rw2G008990 Rw6G020640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 312
AccII CGCG 1 cut(s) 62
AciI CCGC 5 cut(s) 32, 56, 60, 62, 379
AclWI GGATC 2 cut(s) 344, 357
AcoI YGGCCR 2 cut(s) 57, 388
AfiI CCNNNNNNNGG 2 cut(s) 216, 217
AflII CTTAAG 1 cut(s) 98
AgsI TTSAA 3 cut(s) 404, 463, 505
AjnI CCWGG 2 cut(s) 328, 344
AluBI AGCT 3 cut(s) 164, 335, 417
AluI AGCT 3 cut(s) 164, 335, 417
AlwI GGATC 2 cut(s) 344, 357
AlwNI CAGNNNCTG 1 cut(s) 491
AoxI GGCC 3 cut(s) 57, 388, 441
ApeKI GCWGC 4 cut(s) 161, 164, 227, 368
AspS9I GGNCC 2 cut(s) 287, 472
AsuC2I CCSGG 2 cut(s) 180, 470
AsuHPI GGTGA 2 cut(s) 209, 529
AvaII GGWCC 2 cut(s) 287, 472
BamHI GGATCC 1 cut(s) 349
BbvI GCAGC 4 cut(s) 151, 173, 214, 355
BccI CCATC 3 cut(s) 107, 261, 278
BciT130I CCWGG 2 cut(s) 330, 346
BclI TGATCA 1 cut(s) 88
BcnI CCSGG 2 cut(s) 180, 470
BfaI CTAG 1 cut(s) 68
BfrI CTTAAG 1 cut(s) 98
BglI GCCNNNNNGGC 1 cut(s) 56
BisI GCNGC 7 cut(s) 33, 57, 60, 162, 165, 228, 369
BlsI GCNGC 7 cut(s) 34, 58, 61, 163, 166, 229, 370
Bme1390I CCNGG 4 cut(s) 180, 330, 346, 470
Bme18I GGWCC 2 cut(s) 287, 472
BmgT120I GGNCC 2 cut(s) 287, 472
BmiI GGNNCC 1 cut(s) 351
BmrFI CCNGG 4 cut(s) 180, 330, 346, 470
BmrI ACTGGG 1 cut(s) 270
BmsI GCATC 2 cut(s) 112, 139
BmuI ACTGGG 1 cut(s) 270
BoxI GACNNNNGTC 1 cut(s) 533
BpuMI CCSGG 2 cut(s) 180, 470
BsaJI CCNNGG 5 cut(s) 60, 168, 178, 345, 391
Bsc4I CCNNNNNNNGG 2 cut(s) 216, 217
Bse1I ACTGG 2 cut(s) 199, 265
BseBI CCWGG 2 cut(s) 330, 346
BseDI CCNNGG 5 cut(s) 60, 168, 178, 345, 391
BseGI GGATG 2 cut(s) 272, 531
BseLI CCNNNNNNNGG 2 cut(s) 216, 217
BseMII CTCAG 1 cut(s) 546
BseNI ACTGG 2 cut(s) 199, 265
BseX3I CGGCCG 1 cut(s) 57
BseXI GCAGC 4 cut(s) 151, 173, 214, 355
Bsh1236I CGCG 1 cut(s) 62
Bsh1285I CGRYCG 1 cut(s) 60
BshFI GGCC 3 cut(s) 59, 390, 443
BsiEI CGRYCG 1 cut(s) 60
BsiSI CCGG 2 cut(s) 180, 470
BslFI GGGAC 1 cut(s) 286
BslI CCNNNNNNNGG 2 cut(s) 216, 217
BsmFI GGGAC 1 cut(s) 286
BsnI GGCC 3 cut(s) 59, 390, 443
Bsp143I GATC 2 cut(s) 88, 349
BspACI CCGC 5 cut(s) 32, 56, 60, 62, 379
BspANI GGCC 3 cut(s) 59, 390, 443
BspCNI CTCAG 1 cut(s) 545
BspFNI CGCG 1 cut(s) 62
BspHI TCATGA 1 cut(s) 250
BspLI GGNNCC 1 cut(s) 351
BspPI GGATC 2 cut(s) 344, 357
BspTI CTTAAG 1 cut(s) 98
BsrI ACTGG 2 cut(s) 199, 265
BssECI CCNNGG 5 cut(s) 60, 168, 178, 345, 391
BssMI GATC 2 cut(s) 88, 349
BssT1I CCWWGG 1 cut(s) 168
Bst2UI CCWGG 2 cut(s) 330, 346
Bst4CI ACNGT 2 cut(s) 40, 341
BstAFI CTTAAG 1 cut(s) 98
BstAPI GCANNNNNTGC 1 cut(s) 41
BstDEI CTNAG 2 cut(s) 235, 532
BstDSI CCRYGG 1 cut(s) 60
BstF5I GGATG 2 cut(s) 272, 531
BstFNI CGCG 1 cut(s) 62
BstKTI GATC 2 cut(s) 91, 352
BstMBI GATC 2 cut(s) 88, 349
BstMCI CGRYCG 1 cut(s) 60
BstMWI GCNNNNNNNGC 8 cut(s) 11, 41, 56, 158, 170, 236, 341, 449
BstNI CCWGG 2 cut(s) 330, 346
BstPAI GACNNNNGTC 1 cut(s) 533
BstSCI CCNGG 4 cut(s) 178, 328, 344, 468
BstUI CGCG 1 cut(s) 62
BstV1I GCAGC 4 cut(s) 151, 173, 214, 355
BstX2I RGATCY 1 cut(s) 349
BstXI CCANNNNNNTGG 1 cut(s) 297
BstYI RGATCY 1 cut(s) 349
BstZI CGGCCG 1 cut(s) 57
BsuRI GGCC 3 cut(s) 59, 390, 443
BtgI CCRYGG 1 cut(s) 60
BtsCI GGATG 2 cut(s) 272, 531
BtsI GCAGTG 2 cut(s) 201, 437
BtsIMutI CAGTG 2 cut(s) 201, 437
CaiI CAGNNNCTG 1 cut(s) 491
CciI TCATGA 1 cut(s) 250
CciNI GCGGCCGC 1 cut(s) 57
Cfr13I GGNCC 2 cut(s) 287, 472
Cfr42I CCGCGG 1 cut(s) 63
CviAII CATG 5 cut(s) 251, 297, 363, 496, 555
DdeI CTNAG 2 cut(s) 235, 532
DpnI GATC 2 cut(s) 90, 351
DpnII GATC 2 cut(s) 88, 349
EaeI YGGCCR 2 cut(s) 57, 388
EagI CGGCCG 1 cut(s) 57
EclXI CGGCCG 1 cut(s) 57
Eco130I CCWWGG 1 cut(s) 168
Eco47I GGWCC 2 cut(s) 287, 472
Eco52I CGGCCG 1 cut(s) 57
EcoRII CCWGG 2 cut(s) 328, 344
EcoT14I CCWWGG 1 cut(s) 168
ErhI CCWWGG 1 cut(s) 168
FaeI CATG 5 cut(s) 254, 300, 366, 499, 558
FaqI GGGAC 1 cut(s) 286
FatI CATG 5 cut(s) 250, 296, 362, 495, 554
FauI CCCGC 1 cut(s) 55
FbaI TGATCA 1 cut(s) 88
Fnu4HI GCNGC 7 cut(s) 33, 57, 60, 162, 165, 228, 369
FokI GGATG 2 cut(s) 279, 538
Fsp4HI GCNGC 7 cut(s) 33, 57, 60, 162, 165, 228, 369
FspBI CTAG 1 cut(s) 68
GluI GCNGC 7 cut(s) 33, 57, 60, 162, 165, 228, 369
HaeIII GGCC 3 cut(s) 59, 390, 443
HapII CCGG 2 cut(s) 180, 470
Hin1II CATG 5 cut(s) 254, 300, 366, 499, 558
HpaII CCGG 2 cut(s) 180, 470
HphI GGTGA 2 cut(s) 209, 529
Hpy188I TCNGA 1 cut(s) 93
Hpy188III TCNNGA 3 cut(s) 251, 505, 523
HpyAV CCTTC 1 cut(s) 511
HpyCH4III ACNGT 2 cut(s) 40, 341
HpyCH4V TGCA 1 cut(s) 161
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 41, 56, 158, 170, 236, 341, 449
HpyF3I CTNAG 2 cut(s) 235, 532
Hsp92II CATG 5 cut(s) 254, 300, 366, 499, 558
Ksp22I TGATCA 1 cut(s) 88
KspI CCGCGG 1 cut(s) 63
Kzo9I GATC 2 cut(s) 88, 349
LmnI GCTCC 4 cut(s) 319, 332, 414, 449
Lsp1109I GCAGC 4 cut(s) 151, 173, 214, 355
LweI GCATC 2 cut(s) 112, 139
MaeI CTAG 1 cut(s) 68
MaeIII GTNAC 2 cut(s) 197, 535
MalI GATC 2 cut(s) 90, 351
MboI GATC 2 cut(s) 88, 349
MflI RGATCY 1 cut(s) 349
MluCI AATT 1 cut(s) 355
MnlI CCTC 3 cut(s) 386, 541, 557
MseI TTAA 1 cut(s) 99
MspA1I CMGCKG 2 cut(s) 62, 164
MspCI CTTAAG 1 cut(s) 98
MspI CCGG 2 cut(s) 180, 470
MspR9I CCNGG 4 cut(s) 180, 330, 346, 470
MvaI CCWGG 2 cut(s) 330, 346
MvnI CGCG 1 cut(s) 62
MwoI GCNNNNNNNGC 8 cut(s) 11, 41, 56, 158, 170, 236, 341, 449
NciI CCSGG 2 cut(s) 180, 470
NdeII GATC 2 cut(s) 88, 349
NlaIII CATG 5 cut(s) 254, 300, 366, 499, 558
NlaIV GGNNCC 1 cut(s) 351
NmeAIII GCCGAG 1 cut(s) 416
NmuCI GTSAC 2 cut(s) 197, 535
NotI GCGGCCGC 1 cut(s) 57
PagI TCATGA 1 cut(s) 250
PflFI GACNNNGTC 1 cut(s) 491
PfoI TCCNGGA 1 cut(s) 328
PkrI GCNGC 7 cut(s) 34, 58, 61, 163, 166, 229, 370
PshAI GACNNNNGTC 1 cut(s) 533
PsiI TTATAA 1 cut(s) 312
Psp6I CCWGG 2 cut(s) 328, 344
PspGI CCWGG 2 cut(s) 328, 344
PspN4I GGNNCC 1 cut(s) 351
PspPI GGNCC 2 cut(s) 287, 472
PstNI CAGNNNCTG 1 cut(s) 491
PsuI RGATCY 1 cut(s) 349
PsyI GACNNNGTC 1 cut(s) 491
PvuII CAGCTG 1 cut(s) 164
SacII CCGCGG 1 cut(s) 63
SaqAI TTAA 1 cut(s) 99
SatI GCNGC 7 cut(s) 33, 57, 60, 162, 165, 228, 369
Sau3AI GATC 2 cut(s) 88, 349
Sau96I GGNCC 2 cut(s) 287, 472
ScrFI CCNGG 4 cut(s) 180, 330, 346, 470
SetI ASST 9 cut(s) 24, 86, 166, 337, 397, 419, 459, 477, 533
SfaNI GCATC 2 cut(s) 112, 139
Sfr303I CCGCGG 1 cut(s) 63
SgrBI CCGCGG 1 cut(s) 63
SinI GGWCC 2 cut(s) 287, 472
SmlI CTYRAG 1 cut(s) 98
SmoI CTYRAG 1 cut(s) 98
Sse9I AATT 1 cut(s) 355
SsiI CCGC 5 cut(s) 32, 56, 60, 62, 379
SspMI CTAG 1 cut(s) 68
StyD4I CCNGG 4 cut(s) 178, 328, 344, 468
StyI CCWWGG 1 cut(s) 168
TaaI ACNGT 2 cut(s) 40, 341
TaqI TCGA 1 cut(s) 106
TasI AATT 1 cut(s) 355
TauI GCSGC 3 cut(s) 35, 59, 62
Tru1I TTAA 1 cut(s) 99
Tru9I TTAA 1 cut(s) 99
TscAI CASTG 2 cut(s) 208, 437
TseFI GTSAC 2 cut(s) 197, 535
TseI GCWGC 4 cut(s) 161, 164, 227, 368
Tsp45I GTSAC 2 cut(s) 197, 535
TspDTI ATGAA 1 cut(s) 267
TspRI CASTG 2 cut(s) 208, 437
Tth111I GACNNNGTC 1 cut(s) 491
Vha464I CTTAAG 1 cut(s) 98
VpaK11BI GGWCC 2 cut(s) 287, 472
XspI CTAG 1 cut(s) 68
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.