MD02G1120200.v1.1

Endochitinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000495
Physical Location & Seq
Forward (+)
9819345 .. 9821310
1966 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1120200.v1.1.491

Sequence Viewer

Length: 954 bp
ATGAAGTTGCAAGCTCTCATTTTTTTGTCCCTAACTTTGTTGCTAGGAATCTCAGCAGAGCAATGTGGGAGCCAAGCCGGTGGCGCCGTGTGTCCAAATGGGCTGTGTTGTAGCCAGTATGGGTGGTGCGGCACCACATCTGACTACTGCGCCACTGGTTGCCAAAGCCAATGTGGCTCAACCCCTAATCCTACTCCAACCCCAACCCCAAGTGGTGGTGGTGGTGATATCAGCAGCCTCATTAGCTCGTCGGTTTTCGACCAAATGCTGAAGTATCGAAATGATGCACGCTGCCAGAGTAATGGGTTTTACACATATGATGCTTTCGTTGCTGCTGCTCGGTCTTTTAATGGGTTTGGCACAACTGGAGATGATGCTACTCGCAAAAGAGAGCTTGCTGCTTTCTTGGCACAAACCTCTCATGAGACTACTGGAGGATGGGAAAGTGCACCAGATGGTCCATATGCATGGGGATATTGCTTTGTCAATGAAATAAACCAAGATGTGTATTGTTCTTCCAACCAATATCCATGCGCTGCTGGCAAGAAATATTACGGCAGAGGACCCATCCAACTCACCCACAACTACAACTATGCTCAAGCGGGTCAAGCACTAGGACAGGATCTGATAAACAACCCAGATCTAGTGGCTACAGACCCGGTTGTGTCATTCAGGACAGCTATATGGTTTTGGATGACTCCACAGAGCAACAAGCCATCAAGCCATGATGTCATCACTGGTACGTGGAGCCCATCTAGTGCAGACACGTCAGCGGGTCGAGTTCCCGGGTATGGAGTGATCACCAACATCATCAATGGAGGGCTTGAATGTGGCAAGGGTCAGGACGATAGGGTTGCTAGTCGGATTGGATTCTACAGAAGGTACTGTGAGATATTGGGAGTGAGTCCGGGGGACAACTTGGATTGTTACAATCAAAGGCCTTTTGCCCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00520 ko01100 ko04016 map00520 map01100 map04016
Pfam Domains
Protein Families
Chitin_bind_1 Glyco_hydro_19

Protein Analysis

318

Amino Acids

33.96

Weight (kDa)

5.17

Isoelectric Point (pI)

38.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chitin_bind_1 PF00187 21 - 58 3.2e-15 Chitin recognition protein
Glyco_hydro_19 PF00182 80 - 309 1.9e-129 Chitinase class I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000495)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12500
fragaria_vesca FvH4_1g10640 FvH4_1g10640 FvH4_1g10640 FvH4_1g10640 FvH4_1g10640 FvH4_1g10641 FvH4_1g10641 FvH4_1g10642 FvH4_1g10643 FvH4_1g10650 FvH4_1g10660 FvH4_1g10660 FvH4_1g10661 FvH4_2g16190
malus_domestica MD02G1120200.v1.1 MD02G1120300.v1.1 MD15G1234400.v1.1
prunus_persica Prupe.7G178500_v2.0.a1 Prupe.8G174900_v2.0.a1
pyrus_communis pycom02g09370 pycom02g09380 pycom02g09390
rosa_chinensis RchiOBHm_Chr2g0097671 RchiOBHm_Chr2g0097691 RchiOBHm_Chr2g0097731 RchiOBHm_Chr2g0097781 RchiOBHm_Chr2g0097801 RchiOBHm_Chr2g0097821 RchiOBHm_Chr6g0279811 RchiOBHm_Chr6g0279831 RchiOBHm_Chr6g0279841
rosa_laevigata RLG00000013112 RLG00000013114 RLG00000016734 RLG00000016736 RLG00000016737 RLG00000016738
rosa_multiflora Rmu_sc0000974.1_g000004 Rmu_sc0000974.1_g000013 Rmu_sc0000974.1_g000019 Rmu_sc0000974.1_g000025 Rmu_sc0002340.1_g000010 Rmu_sc0008442.1_g000003 Rmu_sc0008442.1_g000009 Rmu_sc0020401.1_g000002
rosa_roxburghii Rroxscaffold_2G00140520 Rroxscaffold_2G00144540 Rroxscaffold_2G00144580 Rroxscaffold_2G00144590 Rroxscaffold_7G00188570 Rroxscaffold_7G00188590 Rroxscaffold_7G00188610
rosa_rugosa Rorug02G0068300 Rorug02G0068400 Rorug06G0128600
rosa_samantha Rh2AG114800 Rh2AG114900 Rh2AG115000 Rh2AG115100 Rh2BG117600 Rh2BG117800 Rh2BG117900 Rh2BG118000 Rh2CG119600 Rh2DG118500 Rh2DG118800 Rh2DG119100 Rh2DG119200 Rh2DG119300 Rh5AG369400 Rh5CG403900 Rh6AG236600 Rh6AG236700 Rh6AG236800 Rh6AG237300 Rh6BG241000 Rh6BG241200 Rh6BG241400 Rh6CG243300 Rh6CG243400 Rh6CG243600 Rh6DG234400 Rh6DG234500 Rh7AG282100
rosa_wichuraiana Rw2G008980 Rw2G008990 Rw6G020640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 83, 131
AccB7I CCANNNNNTGG 1 cut(s) 215
AciI CCGC 3 cut(s) 129, 602, 773
AclWI GGATC 1 cut(s) 630
AcuI CTGAAG 1 cut(s) 290
AcyI GRCGYC 1 cut(s) 84
AfaI GTAC 2 cut(s) 742, 884
AfiI CCNNNNNNNGG 2 cut(s) 215, 791
AflIII ACRYGT 1 cut(s) 765
AgsI TTSAA 1 cut(s) 827
AjiI CACGTC 1 cut(s) 768
AluBI AGCT 4 cut(s) 14, 246, 394, 680
AluI AGCT 4 cut(s) 14, 246, 394, 680
Alw21I GWGCWC 1 cut(s) 451
Alw26I GTCTC 1 cut(s) 419
Alw44I GTGCAC 1 cut(s) 447
AlwI GGATC 1 cut(s) 630
AlwNI CAGNNNCTG 1 cut(s) 625
Ama87I CYCGRG 1 cut(s) 785
AoxI GGCC 1 cut(s) 938
ApaLI GTGCAC 1 cut(s) 447
ApeKI GCWGC 6 cut(s) 234, 291, 332, 335, 398, 536
AspLEI GCGC 3 cut(s) 86, 152, 536
AspS9I GGNCC 2 cut(s) 458, 563
AsuC2I CCSGG 4 cut(s) 659, 786, 787, 909
AsuHPI GGTGA 3 cut(s) 236, 568, 793
AvaI CYCGRG 1 cut(s) 785
AvaII GGWCC 2 cut(s) 458, 563
BaeGI GKGCMC 1 cut(s) 451
BanI GGYRCC 2 cut(s) 83, 131
BanII GRGCYC 1 cut(s) 752
Bbv12I GWGCWC 1 cut(s) 451
BbvI GCAGC 6 cut(s) 246, 278, 319, 322, 385, 523
BccI CCATC 5 cut(s) 432, 449, 575, 724, 760
BceAI ACGGC 2 cut(s) 71, 571
BcgI CGANNNNNNTGC 2 cut(s) 836, 870
BclI TGATCA 1 cut(s) 798
BcnI CCSGG 4 cut(s) 659, 786, 787, 909
BcoDI GTCTC 1 cut(s) 419
BfaI CTAG 5 cut(s) 44, 614, 644, 756, 858
BfmI CTRYAG 2 cut(s) 651, 874
BfoI RGCGCY 1 cut(s) 87
BglI GCCNNNNNGGC 1 cut(s) 174
BglII AGATCT 1 cut(s) 640
BisI GCNGC 7 cut(s) 130, 235, 292, 333, 336, 399, 537
BlsI GCNGC 7 cut(s) 131, 236, 293, 334, 337, 400, 538
Bme1390I CCNGG 4 cut(s) 659, 786, 787, 909
Bme18I GGWCC 2 cut(s) 458, 563
BmeT110I CYCGRG 1 cut(s) 785
BmgBI CACGTC 1 cut(s) 768
BmgT120I GGNCC 2 cut(s) 458, 563
BmiI GGNNCC 5 cut(s) 71, 85, 133, 565, 749
BmrFI CCNGG 4 cut(s) 659, 786, 787, 909
BmsI GCATC 3 cut(s) 274, 310, 364
BpmI CTGGAG 2 cut(s) 387, 453
BpuEI CTTGAG 1 cut(s) 582
BpuMI CCSGG 4 cut(s) 659, 786, 787, 909
BsaAI YACGTR 1 cut(s) 744
BsaHI GRCGYC 1 cut(s) 84
BsaJI CCNNGG 2 cut(s) 785, 908
Bsc4I CCNNNNNNNGG 2 cut(s) 215, 791
Bse118I RCCGGY 1 cut(s) 77
Bse1I ACTGG 5 cut(s) 115, 160, 370, 436, 742
Bse3DI GCAATG 1 cut(s) 68
BseDI CCNNGG 2 cut(s) 785, 908
BseGI GGATG 3 cut(s) 443, 567, 699
BseLI CCNNNNNNNGG 2 cut(s) 215, 791
BseMI GCAATG 1 cut(s) 68
BseMII CTCAG 1 cut(s) 66
BseNI ACTGG 5 cut(s) 115, 160, 370, 436, 742
BseSI GKGCMC 1 cut(s) 451
BseXI GCAGC 6 cut(s) 246, 278, 319, 322, 385, 523
BsgI GTGCAG 1 cut(s) 780
BshFI GGCC 1 cut(s) 940
BshNI GGYRCC 2 cut(s) 83, 131
BsiHKAI GWGCWC 1 cut(s) 451
BsiHKCI CYCGRG 1 cut(s) 785
BsiSI CCGG 4 cut(s) 78, 659, 786, 908
BslFI GGGAC 2 cut(s) 13, 926
BslI CCNNNNNNNGG 2 cut(s) 215, 791
BsmAI GTCTC 1 cut(s) 419
BsmFI GGGAC 2 cut(s) 13, 926
BsnI GGCC 1 cut(s) 940
BsoBI CYCGRG 1 cut(s) 785
Bsp1286I GDGCHC 2 cut(s) 451, 752
Bsp143I GATC 3 cut(s) 622, 640, 798
BspACI CCGC 3 cut(s) 129, 602, 773
BspANI GGCC 1 cut(s) 940
BspCNI CTCAG 1 cut(s) 65
BspHI TCATGA 1 cut(s) 421
BspLI GGNNCC 5 cut(s) 71, 85, 133, 565, 749
BspPI GGATC 1 cut(s) 630
BspT107I GGYRCC 2 cut(s) 83, 131
BsrDI GCAATG 1 cut(s) 68
BsrFI RCCGGY 1 cut(s) 77
BsrI ACTGG 5 cut(s) 115, 160, 370, 436, 742
BssAI RCCGGY 1 cut(s) 77
BssECI CCNNGG 2 cut(s) 785, 908
BssMI GATC 3 cut(s) 622, 640, 798
BssNI GRCGYC 1 cut(s) 84
Bst4CI ACNGT 1 cut(s) 887
BstACI GRCGYC 1 cut(s) 84
BstBAI YACGTR 1 cut(s) 744
BstC8I GCNNGC 4 cut(s) 12, 289, 396, 541
BstDEI CTNAG 1 cut(s) 52
BstF5I GGATG 3 cut(s) 443, 567, 699
BstH2I RGCGCY 1 cut(s) 87
BstHHI GCGC 3 cut(s) 86, 152, 536
BstKTI GATC 3 cut(s) 625, 643, 801
BstMAI GTCTC 1 cut(s) 419
BstMBI GATC 3 cut(s) 622, 640, 798
BstMWI GCNNNNNNNGC 7 cut(s) 83, 174, 243, 329, 407, 540, 608
BstSCI CCNGG 4 cut(s) 657, 784, 785, 907
BstSFI CTRYAG 2 cut(s) 651, 874
BstSLI GKGCMC 1 cut(s) 451
BstV1I GCAGC 6 cut(s) 246, 278, 319, 322, 385, 523
BstX2I RGATCY 2 cut(s) 622, 640
BstXI CCANNNNNNTGG 3 cut(s) 80, 302, 468
BstYI RGATCY 2 cut(s) 622, 640
BsuRI GGCC 1 cut(s) 940
BtrI CACGTC 1 cut(s) 768
BtsCI GGATG 3 cut(s) 443, 567, 699
BtsIMutI CAGTG 2 cut(s) 153, 735
Cac8I GCNNGC 4 cut(s) 12, 289, 396, 541
CaiI CAGNNNCTG 1 cut(s) 625
CciI TCATGA 1 cut(s) 421
CfoI GCGC 3 cut(s) 86, 152, 536
Cfr10I RCCGGY 1 cut(s) 77
Cfr13I GGNCC 2 cut(s) 458, 563
Cfr9I CCCGGG 1 cut(s) 785
Csp6I GTAC 2 cut(s) 741, 883
CviAII CATG 4 cut(s) 422, 468, 531, 725
CviQI GTAC 2 cut(s) 741, 883
DdeI CTNAG 1 cut(s) 52
DinI GGCGCC 1 cut(s) 85
DpnI GATC 3 cut(s) 624, 642, 800
DpnII GATC 3 cut(s) 622, 640, 798
Eco147I AGGCCT 1 cut(s) 940
Eco24I GRGCYC 1 cut(s) 752
Eco32I GATATC 1 cut(s) 229
Eco47I GGWCC 2 cut(s) 458, 563
Eco57I CTGAAG 1 cut(s) 290
Eco88I CYCGRG 1 cut(s) 785
EcoO109I RGGNCCY 1 cut(s) 563
EcoRV GATATC 1 cut(s) 229
EcoT22I ATGCAT 1 cut(s) 469
EcoT38I GRGCYC 1 cut(s) 752
EgeI GGCGCC 1 cut(s) 85
EheI GGCGCC 1 cut(s) 85
FaeI CATG 4 cut(s) 425, 471, 534, 728
FaqI GGGAC 2 cut(s) 13, 926
FatI CATG 4 cut(s) 421, 467, 530, 724
FauI CCCGC 2 cut(s) 595, 766
FauNDI CATATG 2 cut(s) 316, 463
FbaI TGATCA 1 cut(s) 798
Fnu4HI GCNGC 7 cut(s) 130, 235, 292, 333, 336, 399, 537
FokI GGATG 3 cut(s) 450, 554, 706
FriOI GRGCYC 1 cut(s) 752
Fsp4HI GCNGC 7 cut(s) 130, 235, 292, 333, 336, 399, 537
FspBI CTAG 5 cut(s) 44, 614, 644, 756, 858
GlaI GCGC 3 cut(s) 85, 151, 535
GluI GCNGC 7 cut(s) 130, 235, 292, 333, 336, 399, 537
GsuI CTGGAG 2 cut(s) 387, 453
HaeII RGCGCY 1 cut(s) 87
HaeIII GGCC 1 cut(s) 940
HapII CCGG 4 cut(s) 78, 659, 786, 908
HhaI GCGC 3 cut(s) 86, 152, 536
Hin1I GRCGYC 1 cut(s) 84
Hin1II CATG 4 cut(s) 425, 471, 534, 728
Hin6I GCGC 3 cut(s) 84, 150, 534
HinP1I GCGC 3 cut(s) 84, 150, 534
HinfI GANTC 4 cut(s) 48, 697, 870, 904
HpaII CCGG 4 cut(s) 78, 659, 786, 908
HphI GGTGA 3 cut(s) 236, 568, 793
Hpy166II GTNNAC 1 cut(s) 449
Hpy188I TCNGA 3 cut(s) 142, 627, 864
Hpy188III TCNNGA 3 cut(s) 422, 673, 842
Hpy8I GTNNAC 1 cut(s) 449
Hpy99I CGWCG 1 cut(s) 253
HpyAV CCTTC 1 cut(s) 873
HpyCH4III ACNGT 1 cut(s) 887
HpyCH4IV ACGT 2 cut(s) 743, 767
HpyCH4V TGCA 5 cut(s) 10, 287, 449, 467, 761
HpyF10VI GCNNNNNNNGC 7 cut(s) 83, 174, 243, 329, 407, 540, 608
HpyF3I CTNAG 1 cut(s) 52
HpySE526I ACGT 2 cut(s) 743, 767
Hsp92I GRCGYC 1 cut(s) 84
Hsp92II CATG 4 cut(s) 425, 471, 534, 728
HspAI GCGC 3 cut(s) 84, 150, 534
KasI GGCGCC 1 cut(s) 83
Ksp22I TGATCA 1 cut(s) 798
Kzo9I GATC 3 cut(s) 622, 640, 798
LmnI GCTCC 2 cut(s) 69, 747
Lsp1109I GCAGC 6 cut(s) 246, 278, 319, 322, 385, 523
LweI GCATC 3 cut(s) 274, 310, 364
MaeI CTAG 5 cut(s) 44, 614, 644, 756, 858
MaeII ACGT 2 cut(s) 743, 767
MaeIII GTNAC 1 cut(s) 926
MalI GATC 3 cut(s) 624, 642, 800
MboI GATC 3 cut(s) 622, 640, 798
MboII GAAGA 1 cut(s) 507
MflI RGATCY 2 cut(s) 622, 640
MhlI GDGCHC 2 cut(s) 451, 752
Mly113I GGCGCC 1 cut(s) 84
MlyI GAGTC 2 cut(s) 691, 913
MmeI TCCRAC 4 cut(s) 221, 543, 595, 842
MnlI CCTC 5 cut(s) 248, 427, 428, 554, 812
Mph1103I ATGCAT 1 cut(s) 469
MseI TTAA 1 cut(s) 348
MslI CAYNNNNRTG 1 cut(s) 466
MspA1I CMGCKG 1 cut(s) 773
MspI CCGG 4 cut(s) 78, 659, 786, 908
MspR9I CCNGG 4 cut(s) 659, 786, 787, 909
MwoI GCNNNNNNNGC 7 cut(s) 83, 174, 243, 329, 407, 540, 608
NarI GGCGCC 1 cut(s) 84
NciI CCSGG 4 cut(s) 659, 786, 787, 909
NdeI CATATG 2 cut(s) 316, 463
NdeII GATC 3 cut(s) 622, 640, 798
NlaIII CATG 4 cut(s) 425, 471, 534, 728
NlaIV GGNNCC 5 cut(s) 71, 85, 133, 565, 749
NsiI ATGCAT 1 cut(s) 469
PagI TCATGA 1 cut(s) 421
PceI AGGCCT 1 cut(s) 940
PfeI GAWTC 2 cut(s) 48, 870
PflMI CCANNNNNTGG 1 cut(s) 215
PkrI GCNGC 7 cut(s) 131, 236, 293, 334, 337, 400, 538
PleI GAGTC 2 cut(s) 691, 912
PluTI GGCGCC 1 cut(s) 87
PpsI GAGTC 2 cut(s) 691, 912
Ppu21I YACGTR 1 cut(s) 744
PpuMI RGGWCCY 1 cut(s) 563
Psp5II RGGWCCY 1 cut(s) 563
PspN4I GGNNCC 5 cut(s) 71, 85, 133, 565, 749
PspPI GGNCC 2 cut(s) 458, 563
PspPPI RGGWCCY 1 cut(s) 563
PstNI CAGNNNCTG 1 cut(s) 625
PsuI RGATCY 2 cut(s) 622, 640
RsaI GTAC 2 cut(s) 742, 884
RsaNI GTAC 2 cut(s) 741, 883
RseI CAYNNNNRTG 1 cut(s) 466
SaqAI TTAA 1 cut(s) 348
SatI GCNGC 7 cut(s) 130, 235, 292, 333, 336, 399, 537
Sau3AI GATC 3 cut(s) 622, 640, 798
Sau96I GGNCC 2 cut(s) 458, 563
SchI GAGTC 2 cut(s) 691, 913
ScrFI CCNGG 4 cut(s) 659, 786, 787, 909
SduI GDGCHC 2 cut(s) 451, 752
SetI ASST 8 cut(s) 16, 248, 396, 419, 682, 746, 770, 884
SfaNI GCATC 3 cut(s) 274, 310, 364
SfcI CTRYAG 2 cut(s) 651, 874
SfoI GGCGCC 1 cut(s) 85
SinI GGWCC 2 cut(s) 458, 563
SmaI CCCGGG 1 cut(s) 787
SmiMI CAYNNNNRTG 1 cut(s) 466
SmlI CTYRAG 1 cut(s) 597
SmoI CTYRAG 1 cut(s) 597
SseBI AGGCCT 1 cut(s) 940
SsiI CCGC 3 cut(s) 129, 602, 773
SspDI GGCGCC 1 cut(s) 83
SspI AATATT 1 cut(s) 551
SspMI CTAG 5 cut(s) 44, 614, 644, 756, 858
StuI AGGCCT 1 cut(s) 940
StyD4I CCNGG 4 cut(s) 657, 784, 785, 907
TaaI ACNGT 1 cut(s) 887
TaiI ACGT 2 cut(s) 746, 770
TaqI TCGA 3 cut(s) 258, 277, 778
TaqII GACCGA 1 cut(s) 330
TauI GCSGC 1 cut(s) 132
TfiI GAWTC 2 cut(s) 48, 870
Tru1I TTAA 1 cut(s) 348
Tru9I TTAA 1 cut(s) 348
TscAI CASTG 2 cut(s) 160, 742
TseI GCWGC 6 cut(s) 234, 291, 332, 335, 398, 536
TspDTI ATGAA 2 cut(s) 17, 504
TspMI CCCGGG 1 cut(s) 785
TspRI CASTG 2 cut(s) 160, 742
Van91I CCANNNNNTGG 1 cut(s) 215
VneI GTGCAC 1 cut(s) 447
VpaK11BI GGWCC 2 cut(s) 458, 563
XcmI CCANNNNNNNNNTGG 1 cut(s) 170
XmaI CCCGGG 1 cut(s) 785
XspI CTAG 5 cut(s) 44, 614, 644, 756, 858
Zsp2I ATGCAT 1 cut(s) 469
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.