Rroxscaffold_2G00144540

Endochitinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000495
Physical Location & Seq
Reverse (-)
82433244 .. 82441616
8373 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00144540.1

Sequence Viewer

Length: 1038 bp
ATGAAAACCGCGTTCCAGATCGGCTATATATACACCATTAAATCTCACACATACTCCATACCATCAAAAGCCTCCATGAAAATGAAGCTACATTCCTTTATCATCGTATCCCTCATTTTGTTGCTAGGTATCTCCGCAGAACAATGCGGTAGCCAAGCCGGTGGTGCTACCTGCCCGAATGGGCTATGTTGTAGCGAGTTTGGCTGGTGTGGCAGCACAGCCGATTACTGTACTAAAAACTGCCAAAGCCAATGTAGTTCAGGCGGTGGCTCAACCCCTTCTCCTACTCCAAGTGGCGGCGGCGGCGGCGGCGATGTTGCCGGCCTCGTTAGCTCCTCTCTCTTCGATCAAATGCTTAAGTATCGCAACGATGCACGGTGCAAGGGCAACGGTTTCTACAAGTATGATGCTTTCATTGCTGCTGCTCGATCATTCCCTGGGTTTGGCACCACCGGAGATGATGCAACCCGCAAGAAGGAGCTCGCTGCTTTCTTAGCTCAAACCTCTCATGAGACCACTGGTGGATGGCCAAGTGCACCAGATGGTCCTTACGCATGGGGTTATTGCTTTGTCAATGAAAACGACCAGGATGTGCATTGTACAAACCCAGAATATCCATGTGCTCCTGGCAAGAAATACTATGGTCGAGGACCCATTCAACTCACCCACAATTACAACTATGATCAAGCGGGTAAAGCAATCGAAGCGGATCTAATAAACAATCCGGACCTAGTGGCCACAGACCCAGTTGTATCATTCAAGACAGCAATATGGTTTTGGATGACTCCACAATCAAACAAACCATCAAGCCACGACGTCGTTACCGGCCGTTGGAACCCATCTGATGCAGATAAATCCGCGGGTCGGGTCCCTGGATATGGTGTGATTACTAACATCATCAATGGTGGACTTGAATGTGGGCATGGCCAAGATGATAAGGTGGCGGATAGGATCGGGTTCTATACGAGGTACACGGGCATATTGGGAGTGAGCCCTGGAGGCAACTTGGATTGCTATAACCAGAGGCCCTTTGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00520 ko01100 ko04016 map00520 map01100 map04016
Pfam Domains
Protein Families
Chitin_bind_1 Glyco_hydro_19

Protein Analysis

345

Amino Acids

36.86

Weight (kDa)

6.63

Isoelectric Point (pI)

37.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chitin_bind_1 PF00187 48 - 85 7e-14 Chitin recognition protein
Glyco_hydro_19 PF00182 109 - 338 1.2e-126 Chitinase class I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000495)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12500
fragaria_vesca FvH4_1g10640 FvH4_1g10640 FvH4_1g10640 FvH4_1g10640 FvH4_1g10640 FvH4_1g10641 FvH4_1g10641 FvH4_1g10642 FvH4_1g10643 FvH4_1g10650 FvH4_1g10660 FvH4_1g10660 FvH4_1g10661 FvH4_2g16190
malus_domestica MD02G1120200.v1.1 MD02G1120300.v1.1 MD15G1234400.v1.1
prunus_persica Prupe.7G178500_v2.0.a1 Prupe.8G174900_v2.0.a1
pyrus_communis pycom02g09370 pycom02g09380 pycom02g09390
rosa_chinensis RchiOBHm_Chr2g0097671 RchiOBHm_Chr2g0097691 RchiOBHm_Chr2g0097731 RchiOBHm_Chr2g0097781 RchiOBHm_Chr2g0097801 RchiOBHm_Chr2g0097821 RchiOBHm_Chr6g0279811 RchiOBHm_Chr6g0279831 RchiOBHm_Chr6g0279841
rosa_laevigata RLG00000013112 RLG00000013114 RLG00000016734 RLG00000016736 RLG00000016737 RLG00000016738
rosa_multiflora Rmu_sc0000974.1_g000004 Rmu_sc0000974.1_g000013 Rmu_sc0000974.1_g000019 Rmu_sc0000974.1_g000025 Rmu_sc0002340.1_g000010 Rmu_sc0008442.1_g000003 Rmu_sc0008442.1_g000009 Rmu_sc0020401.1_g000002
rosa_roxburghii Rroxscaffold_2G00140520 Rroxscaffold_2G00144540 Rroxscaffold_2G00144580 Rroxscaffold_2G00144590 Rroxscaffold_7G00188570 Rroxscaffold_7G00188590 Rroxscaffold_7G00188610
rosa_rugosa Rorug02G0068300 Rorug02G0068400 Rorug06G0128600
rosa_samantha Rh2AG114800 Rh2AG114900 Rh2AG115000 Rh2AG115100 Rh2BG117600 Rh2BG117800 Rh2BG117900 Rh2BG118000 Rh2CG119600 Rh2DG118500 Rh2DG118800 Rh2DG119100 Rh2DG119200 Rh2DG119300 Rh5AG369400 Rh5CG403900 Rh6AG236600 Rh6AG236700 Rh6AG236800 Rh6AG237300 Rh6BG241000 Rh6BG241200 Rh6BG241400 Rh6CG243300 Rh6CG243400 Rh6CG243600 Rh6DG234400 Rh6DG234500 Rh7AG282100
rosa_wichuraiana Rw2G008980 Rw2G008990 Rw6G020640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 819
Acc36I ACCTGC 1 cut(s) 179
AccB1I GGYRCC 1 cut(s) 446
AccII CGCG 2 cut(s) 11, 860
AccIII TCCGGA 1 cut(s) 724
AclWI GGATC 2 cut(s) 717, 959
AcoI YGGCCR 4 cut(s) 527, 735, 826, 925
AcyI GRCGYC 1 cut(s) 816
AfaI GTAC 3 cut(s) 232, 601, 971
AfiI CCNNNNNNNGG 6 cut(s) 296, 443, 475, 831, 864, 878
AflII CTTAAG 1 cut(s) 356
AgsI TTSAA 3 cut(s) 659, 760, 914
AjnI CCWGG 5 cut(s) 436, 585, 625, 871, 994
AluBI AGCT 4 cut(s) 88, 333, 481, 497
AluI AGCT 4 cut(s) 88, 333, 481, 497
Alw21I GWGCWC 3 cut(s) 483, 538, 625
Alw26I GTCTC 1 cut(s) 506
Alw44I GTGCAC 1 cut(s) 534
AlwI GGATC 2 cut(s) 717, 959
Aor13HI TCCGGA 1 cut(s) 724
AoxI GGCC 6 cut(s) 322, 527, 735, 826, 925, 1025
ApaLI GTGCAC 1 cut(s) 534
ApeKI GCWGC 4 cut(s) 213, 419, 422, 485
AspS9I GGNCC 5 cut(s) 545, 650, 727, 868, 1026
AsuHPI GGTGA 1 cut(s) 655
AvaII GGWCC 4 cut(s) 545, 650, 727, 868
BaeGI GKGCMC 1 cut(s) 538
BalI TGGCCA 3 cut(s) 529, 737, 927
BanI GGYRCC 1 cut(s) 446
BanII GRGCYC 2 cut(s) 483, 995
Bbv12I GWGCWC 3 cut(s) 483, 538, 625
BbvI GCAGC 4 cut(s) 225, 406, 409, 472
BccI CCATC 5 cut(s) 70, 519, 536, 811, 847
BceAI ACGGC 1 cut(s) 813
BciT130I CCWGG 5 cut(s) 438, 587, 627, 873, 996
BciVI GTATCC 1 cut(s) 118
BclI TGATCA 1 cut(s) 682
BcoDI GTCTC 1 cut(s) 506
BfaI CTAG 2 cut(s) 125, 731
BfrI CTTAAG 1 cut(s) 356
BfuAI ACCTGC 1 cut(s) 179
BfuI GTATCC 1 cut(s) 118
BglI GCCNNNNNGGC 1 cut(s) 999
BisI GCNGC 9 cut(s) 214, 298, 301, 304, 307, 310, 420, 423, 486
BlsI GCNGC 9 cut(s) 215, 299, 302, 305, 308, 311, 421, 424, 487
Bme1390I CCNGG 5 cut(s) 438, 587, 627, 873, 996
Bme18I GGWCC 4 cut(s) 545, 650, 727, 868
BmgT120I GGNCC 5 cut(s) 545, 650, 727, 868, 1026
BmiI GGNNCC 5 cut(s) 448, 652, 836, 869, 870
BmrFI CCNGG 5 cut(s) 438, 587, 627, 873, 996
BmrI ACTGGG 1 cut(s) 740
BmsI GCATC 4 cut(s) 361, 397, 451, 835
BmuI ACTGGG 1 cut(s) 740
BpmI CTGGAG 1 cut(s) 1017
BsaHI GRCGYC 1 cut(s) 816
BsaI GGTCTC 1 cut(s) 506
BsaJI CCNNGG 5 cut(s) 436, 437, 858, 871, 994
BsaWI WCCGGW 2 cut(s) 452, 724
BsaXI ACNNNNNCTCC 4 cut(s) 38, 68, 265, 295
Bsc4I CCNNNNNNNGG 6 cut(s) 296, 443, 475, 831, 864, 878
Bse118I RCCGGY 3 cut(s) 158, 320, 824
Bse1I ACTGG 2 cut(s) 523, 746
Bse3DI GCAATG 1 cut(s) 414
BseAI TCCGGA 1 cut(s) 724
BseBI CCWGG 5 cut(s) 438, 587, 627, 873, 996
BseDI CCNNGG 5 cut(s) 436, 437, 858, 871, 994
BseGI GGATG 3 cut(s) 530, 595, 786
BseLI CCNNNNNNNGG 6 cut(s) 296, 443, 475, 831, 864, 878
BseMI GCAATG 1 cut(s) 414
BseNI ACTGG 2 cut(s) 523, 746
BseRI GAGGAG 1 cut(s) 325
BseSI GKGCMC 1 cut(s) 538
BseX3I CGGCCG 1 cut(s) 826
BseXI GCAGC 4 cut(s) 225, 406, 409, 472
Bsh1236I CGCG 2 cut(s) 11, 860
Bsh1285I CGRYCG 1 cut(s) 829
BshFI GGCC 6 cut(s) 324, 529, 737, 828, 927, 1027
BshNI GGYRCC 1 cut(s) 446
BsiEI CGRYCG 1 cut(s) 829
BsiHKAI GWGCWC 3 cut(s) 483, 538, 625
BsiSI CCGG 5 cut(s) 159, 321, 453, 725, 825
BslFI GGGAC 1 cut(s) 854
BslI CCNNNNNNNGG 6 cut(s) 296, 443, 475, 831, 864, 878
BsmAI GTCTC 1 cut(s) 506
BsmFI GGGAC 1 cut(s) 854
BsnI GGCC 6 cut(s) 324, 529, 737, 828, 927, 1027
Bso31I GGTCTC 1 cut(s) 506
Bsp1286I GDGCHC 4 cut(s) 483, 538, 625, 995
Bsp13I TCCGGA 1 cut(s) 724
Bsp1407I TGTACA 1 cut(s) 599
Bsp143I GATC 6 cut(s) 18, 346, 428, 682, 709, 951
BspANI GGCC 6 cut(s) 324, 529, 737, 828, 927, 1027
BspEI TCCGGA 1 cut(s) 724
BspFNI CGCG 2 cut(s) 11, 860
BspHI TCATGA 1 cut(s) 508
BspLI GGNNCC 5 cut(s) 448, 652, 836, 869, 870
BspMI ACCTGC 1 cut(s) 179
BspPI GGATC 2 cut(s) 717, 959
BspT107I GGYRCC 1 cut(s) 446
BspTI CTTAAG 1 cut(s) 356
BspTNI GGTCTC 1 cut(s) 506
BsrDI GCAATG 1 cut(s) 414
BsrFI RCCGGY 3 cut(s) 158, 320, 824
BsrGI TGTACA 1 cut(s) 599
BsrI ACTGG 2 cut(s) 523, 746
BssAI RCCGGY 3 cut(s) 158, 320, 824
BssECI CCNNGG 5 cut(s) 436, 437, 858, 871, 994
BssMI GATC 6 cut(s) 18, 346, 428, 682, 709, 951
BssNI GRCGYC 1 cut(s) 816
Bst2UI CCWGG 5 cut(s) 438, 587, 627, 873, 996
Bst4CI ACNGT 3 cut(s) 230, 378, 392
Bst6I CTCTTC 1 cut(s) 347
BstACI GRCGYC 1 cut(s) 816
BstAFI CTTAAG 1 cut(s) 356
BstAUI TGTACA 1 cut(s) 599
BstC8I GCNNGC 2 cut(s) 322, 483
BstDEI CTNAG 1 cut(s) 493
BstDSI CCRYGG 1 cut(s) 858
BstF5I GGATG 3 cut(s) 530, 595, 786
BstFNI CGCG 2 cut(s) 11, 860
BstKTI GATC 6 cut(s) 21, 349, 431, 685, 712, 954
BstMAI GTCTC 1 cut(s) 506
BstMBI GATC 6 cut(s) 18, 346, 428, 682, 709, 951
BstMCI CGRYCG 1 cut(s) 829
BstNI CCWGG 5 cut(s) 438, 587, 627, 873, 996
BstSCI CCNGG 5 cut(s) 436, 585, 625, 871, 994
BstSLI GKGCMC 1 cut(s) 538
BstUI CGCG 2 cut(s) 11, 860
BstV1I GCAGC 4 cut(s) 225, 406, 409, 472
BstX2I RGATCY 1 cut(s) 709
BstXI CCANNNNNNTGG 1 cut(s) 161
BstYI RGATCY 1 cut(s) 709
BstZI CGGCCG 1 cut(s) 826
BsuI GTATCC 1 cut(s) 118
BsuRI GGCC 6 cut(s) 324, 529, 737, 828, 927, 1027
BtgI CCRYGG 1 cut(s) 858
BtgZI GCGATG 1 cut(s) 327
BtsCI GGATG 3 cut(s) 530, 595, 786
BtsIMutI CAGTG 1 cut(s) 516
BveI ACCTGC 1 cut(s) 179
Cac8I GCNNGC 2 cut(s) 322, 483
CciI TCATGA 1 cut(s) 508
Cfr10I RCCGGY 3 cut(s) 158, 320, 824
Cfr13I GGNCC 5 cut(s) 545, 650, 727, 868, 1026
Cfr42I CCGCGG 1 cut(s) 861
Csp6I GTAC 3 cut(s) 231, 600, 970
CviAII CATG 5 cut(s) 76, 509, 555, 618, 923
CviQI GTAC 3 cut(s) 231, 600, 970
DdeI CTNAG 1 cut(s) 493
DpnI GATC 6 cut(s) 20, 348, 430, 684, 711, 953
DpnII GATC 6 cut(s) 18, 346, 428, 682, 709, 951
EaeI YGGCCR 4 cut(s) 527, 735, 826, 925
EagI CGGCCG 1 cut(s) 826
Eam1104I CTCTTC 1 cut(s) 347
EarI CTCTTC 1 cut(s) 347
EciI GGCGGA 1 cut(s) 959
Ecl136II GAGCTC 1 cut(s) 481
EclXI CGGCCG 1 cut(s) 826
Eco24I GRGCYC 2 cut(s) 483, 995
Eco31I GGTCTC 1 cut(s) 506
Eco47I GGWCC 4 cut(s) 545, 650, 727, 868
Eco52I CGGCCG 1 cut(s) 826
Eco53kI GAGCTC 1 cut(s) 481
EcoICRI GAGCTC 1 cut(s) 481
EcoO109I RGGNCCY 3 cut(s) 650, 868, 1026
EcoRII CCWGG 5 cut(s) 436, 585, 625, 871, 994
EcoT38I GRGCYC 2 cut(s) 483, 995
FaeI CATG 5 cut(s) 79, 512, 558, 621, 926
FaqI GGGAC 1 cut(s) 854
FatI CATG 5 cut(s) 75, 508, 554, 617, 922
FauI CCCGC 3 cut(s) 476, 682, 853
FbaI TGATCA 1 cut(s) 682
Fnu4HI GCNGC 9 cut(s) 214, 298, 301, 304, 307, 310, 420, 423, 486
FokI GGATG 3 cut(s) 537, 602, 793
FriOI GRGCYC 2 cut(s) 483, 995
Fsp4HI GCNGC 9 cut(s) 214, 298, 301, 304, 307, 310, 420, 423, 486
FspBI CTAG 2 cut(s) 125, 731
GluI GCNGC 9 cut(s) 214, 298, 301, 304, 307, 310, 420, 423, 486
GsuI CTGGAG 1 cut(s) 1017
HaeIII GGCC 6 cut(s) 324, 529, 737, 828, 927, 1027
HapII CCGG 5 cut(s) 159, 321, 453, 725, 825
Hin1I GRCGYC 1 cut(s) 816
Hin1II CATG 5 cut(s) 79, 512, 558, 621, 926
HinfI GANTC 1 cut(s) 784
HpaII CCGG 5 cut(s) 159, 321, 453, 725, 825
HphI GGTGA 1 cut(s) 655
Hpy166II GTNNAC 3 cut(s) 536, 908, 972
Hpy188I TCNGA 1 cut(s) 844
Hpy188III TCNNGA 4 cut(s) 16, 509, 725, 760
Hpy8I GTNNAC 3 cut(s) 536, 908, 972
Hpy99I CGWCG 2 cut(s) 818, 821
HpyAV CCTTC 2 cut(s) 288, 469
HpyCH4III ACNGT 3 cut(s) 230, 378, 392
HpyCH4IV ACGT 1 cut(s) 816
HpyCH4V TGCA 6 cut(s) 374, 381, 464, 536, 595, 848
HpyF3I CTNAG 1 cut(s) 493
HpySE526I ACGT 1 cut(s) 816
Hsp92I GRCGYC 1 cut(s) 816
Hsp92II CATG 5 cut(s) 79, 512, 558, 621, 926
KflI GGGWCCC 1 cut(s) 868
Kpn2I TCCGGA 1 cut(s) 724
KroI GCCGGC 1 cut(s) 320
KroNI GCCGGC 1 cut(s) 322
Ksp22I TGATCA 1 cut(s) 682
KspI CCGCGG 1 cut(s) 861
Kzo9I GATC 6 cut(s) 18, 346, 428, 682, 709, 951
LmnI GCTCC 3 cut(s) 338, 478, 628
Lsp1109I GCAGC 4 cut(s) 225, 406, 409, 472
LweI GCATC 4 cut(s) 361, 397, 451, 835
MaeI CTAG 2 cut(s) 125, 731
MaeII ACGT 1 cut(s) 816
MaeIII GTNAC 1 cut(s) 820
MalI GATC 6 cut(s) 20, 348, 430, 684, 711, 953
MboI GATC 6 cut(s) 18, 346, 428, 682, 709, 951
MboII GAAGA 1 cut(s) 334
MflI RGATCY 1 cut(s) 709
MhlI GDGCHC 4 cut(s) 483, 538, 625, 995
MlsI TGGCCA 3 cut(s) 529, 737, 927
MluCI AATT 1 cut(s) 670
MluNI TGGCCA 3 cut(s) 529, 737, 927
MlyI GAGTC 1 cut(s) 778
MmeI TCCRAC 1 cut(s) 812
MnlI CCTC 9 cut(s) 82, 122, 335, 346, 514, 641, 960, 992, 1017
Mox20I TGGCCA 3 cut(s) 529, 737, 927
MroI TCCGGA 1 cut(s) 724
MroNI GCCGGC 1 cut(s) 320
MscI TGGCCA 3 cut(s) 529, 737, 927
MseI TTAA 2 cut(s) 39, 357
MslI CAYNNNNRTG 1 cut(s) 80
Msp20I TGGCCA 3 cut(s) 529, 737, 927
MspA1I CMGCKG 1 cut(s) 860
MspCI CTTAAG 1 cut(s) 356
MspI CCGG 5 cut(s) 159, 321, 453, 725, 825
MspR9I CCNGG 5 cut(s) 438, 587, 627, 873, 996
MvaI CCWGG 5 cut(s) 438, 587, 627, 873, 996
MvnI CGCG 2 cut(s) 11, 860
NaeI GCCGGC 1 cut(s) 322
NdeII GATC 6 cut(s) 18, 346, 428, 682, 709, 951
NgoMIV GCCGGC 1 cut(s) 320
NlaIII CATG 5 cut(s) 79, 512, 558, 621, 926
NlaIV GGNNCC 5 cut(s) 448, 652, 836, 869, 870
PagI TCATGA 1 cut(s) 508
PasI CCCWGGG 1 cut(s) 437
PdiI GCCGGC 1 cut(s) 322
PkrI GCNGC 9 cut(s) 215, 299, 302, 305, 308, 311, 421, 424, 487
PleI GAGTC 1 cut(s) 778
PpsI GAGTC 1 cut(s) 778
PpuMI RGGWCCY 2 cut(s) 650, 868
Psp124BI GAGCTC 1 cut(s) 483
Psp5II RGGWCCY 2 cut(s) 650, 868
Psp6I CCWGG 5 cut(s) 436, 585, 625, 871, 994
PspGI CCWGG 5 cut(s) 436, 585, 625, 871, 994
PspN4I GGNNCC 5 cut(s) 448, 652, 836, 869, 870
PspPI GGNCC 5 cut(s) 545, 650, 727, 868, 1026
PspPPI RGGWCCY 2 cut(s) 650, 868
PsuI RGATCY 1 cut(s) 709
RsaI GTAC 3 cut(s) 232, 601, 971
RsaNI GTAC 3 cut(s) 231, 600, 970
RseI CAYNNNNRTG 1 cut(s) 80
SacI GAGCTC 1 cut(s) 483
SacII CCGCGG 1 cut(s) 861
SaqAI TTAA 2 cut(s) 39, 357
SatI GCNGC 9 cut(s) 214, 298, 301, 304, 307, 310, 420, 423, 486
Sau3AI GATC 6 cut(s) 18, 346, 428, 682, 709, 951
Sau96I GGNCC 5 cut(s) 545, 650, 727, 868, 1026
SchI GAGTC 1 cut(s) 778
ScrFI CCNGG 5 cut(s) 438, 587, 627, 873, 996
SduI GDGCHC 4 cut(s) 483, 538, 625, 995
SfaNI GCATC 4 cut(s) 361, 397, 451, 835
Sfr303I CCGCGG 1 cut(s) 861
SgrBI CCGCGG 1 cut(s) 861
SinI GGWCC 4 cut(s) 545, 650, 727, 868
SmiMI CAYNNNNRTG 1 cut(s) 80
SmlI CTYRAG 1 cut(s) 356
SmoI CTYRAG 1 cut(s) 356
Sse9I AATT 1 cut(s) 670
SspMI CTAG 2 cut(s) 125, 731
SstI GAGCTC 1 cut(s) 483
StyD4I CCNGG 5 cut(s) 436, 585, 625, 871, 994
TaaI ACNGT 3 cut(s) 230, 378, 392
TaiI ACGT 1 cut(s) 819
TaqI TCGA 4 cut(s) 345, 427, 646, 702
TasI AATT 1 cut(s) 670
TatI WGTACW 2 cut(s) 230, 599
TauI GCSGC 5 cut(s) 300, 303, 306, 309, 312
Tru1I TTAA 2 cut(s) 39, 357
Tru9I TTAA 2 cut(s) 39, 357
TscAI CASTG 1 cut(s) 523
TseI GCWGC 4 cut(s) 213, 419, 422, 485
TspDTI ATGAA 5 cut(s) 17, 92, 98, 403, 591
TspRI CASTG 1 cut(s) 523
Vha464I CTTAAG 1 cut(s) 356
VneI GTGCAC 1 cut(s) 534
VpaK11BI GGWCC 4 cut(s) 545, 650, 727, 868
XspI CTAG 2 cut(s) 125, 731
ZraI GACGTC 1 cut(s) 817
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.