MD02G1120300.v1.1

Endochitinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000495
Physical Location & Seq
Forward (+)
9826361 .. 9828059
1699 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1120300.v1.1.491

Sequence Viewer

Length: 855 bp
ATGAAGTTGCAAACCCTTATCCTGTTGTCCCTAAGTTTGCTGATTGGGATCTCAGCAGAGCAATGTGGGAGGCAAGCTGGTGGTGCCGTGTGCCCAGCTCGAACTCCAACCCCAAGTGGCGGTGGTGACATCAACAGCCTCGTTAGCTCGTCTGTTTTCGACCAAATGCTTAAGTATCGAAACGATGGGAGATGCCCTAGTCATGGGTTTTACAAGTATGGTGCTTTCATTGCTGCAGCTCGGTCTTTTAATGGGTTTGGCACAACTGGAGATGTTAATACTCGCAAAAAGGAGCTTGCTGCTTTCTTGGCTCAAACCTCTCATGAGACTACTGGAGGATGGGCAAGTGCACCAGATGGTCCTTATGCATGGGGATATTGCTTTGTCAATGAAACAAACCAAGATGTGTATTGCACACCATCCGGTCAATATCCATGTGCTGCTGGCAAGAAATACTATGGCAGAGGACCCATCCAACTTACCTACAACTACAACTATGGTCAAGCGGGCAAGGCAATCGGAAATGATCTGATAAAAAACCCGGATCTAGTGGCCACGGACCCGGTTGTATCATTCAAGACAGCTATATGGTTCTGGATGACTCCACAGGGAAACAAGCCATCAAGCCATGATGTCATCACTGGTAGGTGGAGTCCATCTAGTGCAGACAGATCAGCGGGTCGGGTTCCCGGGTATGGAGTGATCACCAACATCATCAACGGAGGGCTTGAATGCGGGAAGGGTCAGGATGCTAGGGTTGCTAGTCGCATCGGCTTCTACAGAAGGTACTGTGAGATATTGGGAGTGAGTCCGGGGGACAACTTGGATTGTTACAATCAAAGGCCTTTTGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00520 ko01100 ko04016 map00520 map01100 map04016
Pfam Domains
Protein Families
Chitin_bind_1 Glyco_hydro_19

Protein Analysis

285

Amino Acids

30.55

Weight (kDa)

8.9

Isoelectric Point (pI)

36.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_19 PF00182 47 - 277 2.1e-131 Chitinase class I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000495)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12500
fragaria_vesca FvH4_1g10640 FvH4_1g10640 FvH4_1g10640 FvH4_1g10640 FvH4_1g10640 FvH4_1g10641 FvH4_1g10641 FvH4_1g10642 FvH4_1g10643 FvH4_1g10650 FvH4_1g10660 FvH4_1g10660 FvH4_1g10661 FvH4_2g16190
malus_domestica MD02G1120200.v1.1 MD02G1120300.v1.1 MD15G1234400.v1.1
prunus_persica Prupe.7G178500_v2.0.a1 Prupe.8G174900_v2.0.a1
pyrus_communis pycom02g09370 pycom02g09380 pycom02g09390
rosa_chinensis RchiOBHm_Chr2g0097671 RchiOBHm_Chr2g0097691 RchiOBHm_Chr2g0097731 RchiOBHm_Chr2g0097781 RchiOBHm_Chr2g0097801 RchiOBHm_Chr2g0097821 RchiOBHm_Chr6g0279811 RchiOBHm_Chr6g0279831 RchiOBHm_Chr6g0279841
rosa_laevigata RLG00000013112 RLG00000013114 RLG00000016734 RLG00000016736 RLG00000016737 RLG00000016738
rosa_multiflora Rmu_sc0000974.1_g000004 Rmu_sc0000974.1_g000013 Rmu_sc0000974.1_g000019 Rmu_sc0000974.1_g000025 Rmu_sc0002340.1_g000010 Rmu_sc0008442.1_g000003 Rmu_sc0008442.1_g000009 Rmu_sc0020401.1_g000002
rosa_roxburghii Rroxscaffold_2G00140520 Rroxscaffold_2G00144540 Rroxscaffold_2G00144580 Rroxscaffold_2G00144590 Rroxscaffold_7G00188570 Rroxscaffold_7G00188590 Rroxscaffold_7G00188610
rosa_rugosa Rorug02G0068300 Rorug02G0068400 Rorug06G0128600
rosa_samantha Rh2AG114800 Rh2AG114900 Rh2AG115000 Rh2AG115100 Rh2BG117600 Rh2BG117800 Rh2BG117900 Rh2BG118000 Rh2CG119600 Rh2DG118500 Rh2DG118800 Rh2DG119100 Rh2DG119200 Rh2DG119300 Rh5AG369400 Rh5CG403900 Rh6AG236600 Rh6AG236700 Rh6AG236800 Rh6AG237300 Rh6BG241000 Rh6BG241200 Rh6BG241400 Rh6CG243300 Rh6CG243400 Rh6CG243600 Rh6DG234400 Rh6DG234500 Rh7AG282100
rosa_wichuraiana Rw2G008980 Rw2G008990 Rw6G020640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 83
AciI CCGC 4 cut(s) 120, 506, 677, 735
AclWI GGATC 2 cut(s) 56, 552
AcoI YGGCCR 1 cut(s) 552
AfaI GTAC 1 cut(s) 788
AfiI CCNNNNNNNGG 3 cut(s) 119, 203, 695
AflII CTTAAG 1 cut(s) 170
AgsI TTSAA 2 cut(s) 577, 731
AluBI AGCT 6 cut(s) 77, 98, 147, 239, 295, 584
AluI AGCT 6 cut(s) 77, 98, 147, 239, 295, 584
Alw21I GWGCWC 1 cut(s) 352
Alw26I GTCTC 1 cut(s) 320
Alw44I GTGCAC 1 cut(s) 348
AlwI GGATC 2 cut(s) 56, 552
Ama87I CYCGRG 1 cut(s) 689
AoxI GGCC 2 cut(s) 552, 842
ApaLI GTGCAC 1 cut(s) 348
ApeKI GCWGC 4 cut(s) 233, 236, 299, 440
AspS9I GGNCC 3 cut(s) 359, 467, 559
AsuC2I CCSGG 5 cut(s) 542, 563, 690, 691, 813
AsuHPI GGTGA 2 cut(s) 137, 697
AvaI CYCGRG 1 cut(s) 689
AvaII GGWCC 3 cut(s) 359, 467, 559
BaeGI GKGCMC 2 cut(s) 95, 352
BalI TGGCCA 1 cut(s) 554
BanI GGYRCC 1 cut(s) 83
Bbv12I GWGCWC 1 cut(s) 352
BbvI GCAGC 4 cut(s) 220, 248, 286, 427
BccI CCATC 7 cut(s) 179, 333, 350, 427, 479, 628, 664
BceAI ACGGC 1 cut(s) 71
BcgI CGANNNNNNTGC 2 cut(s) 499, 533
BclI TGATCA 1 cut(s) 702
BcnI CCSGG 5 cut(s) 542, 563, 690, 691, 813
BcoDI GTCTC 1 cut(s) 320
BfaI CTAG 5 cut(s) 198, 548, 660, 753, 762
BfmI CTRYAG 2 cut(s) 234, 778
BfrI CTTAAG 1 cut(s) 170
BisI GCNGC 4 cut(s) 234, 237, 300, 441
BlsI GCNGC 4 cut(s) 235, 238, 301, 442
Bme1390I CCNGG 5 cut(s) 542, 563, 690, 691, 813
Bme18I GGWCC 3 cut(s) 359, 467, 559
BmeT110I CYCGRG 1 cut(s) 689
BmgT120I GGNCC 3 cut(s) 359, 467, 559
BmiI GGNNCC 4 cut(s) 85, 469, 561, 687
BmrFI CCNGG 5 cut(s) 542, 563, 690, 691, 813
BmsI GCATC 3 cut(s) 182, 739, 777
BpmI CTGGAG 2 cut(s) 288, 354
BpuMI CCSGG 5 cut(s) 542, 563, 690, 691, 813
BsaBI GATNNNNATC 1 cut(s) 47
BsaJI CCNNGG 3 cut(s) 555, 689, 812
BsaWI WCCGGW 1 cut(s) 422
Bsc4I CCNNNNNNNGG 3 cut(s) 119, 203, 695
Bse1I ACTGG 3 cut(s) 271, 337, 646
Bse3DI GCAATG 2 cut(s) 68, 228
Bse8I GATNNNNATC 1 cut(s) 47
BseDI CCNNGG 3 cut(s) 555, 689, 812
BseGI GGATG 5 cut(s) 344, 419, 471, 603, 754
BseJI GATNNNNATC 1 cut(s) 47
BseLI CCNNNNNNNGG 3 cut(s) 119, 203, 695
BseMI GCAATG 2 cut(s) 68, 228
BseMII CTCAG 1 cut(s) 66
BseNI ACTGG 3 cut(s) 271, 337, 646
BseSI GKGCMC 2 cut(s) 95, 352
BseXI GCAGC 4 cut(s) 220, 248, 286, 427
BseYI CCCAGC 1 cut(s) 94
BsgI GTGCAG 1 cut(s) 684
BshFI GGCC 2 cut(s) 554, 844
BshNI GGYRCC 1 cut(s) 83
BsiHKAI GWGCWC 1 cut(s) 352
BsiHKCI CYCGRG 1 cut(s) 689
BsiSI CCGG 5 cut(s) 423, 542, 563, 690, 812
BslFI GGGAC 2 cut(s) 13, 830
BslI CCNNNNNNNGG 3 cut(s) 119, 203, 695
BsmAI GTCTC 1 cut(s) 320
BsmFI GGGAC 2 cut(s) 13, 830
BsmI GAATGC 1 cut(s) 737
BsnI GGCC 2 cut(s) 554, 844
BsoBI CYCGRG 1 cut(s) 689
Bsp1286I GDGCHC 2 cut(s) 95, 352
Bsp143I GATC 5 cut(s) 48, 526, 544, 671, 702
BspACI CCGC 4 cut(s) 120, 506, 677, 735
BspANI GGCC 2 cut(s) 554, 844
BspCNI CTCAG 1 cut(s) 65
BspHI TCATGA 1 cut(s) 322
BspLI GGNNCC 4 cut(s) 85, 469, 561, 687
BspMAI CTGCAG 1 cut(s) 238
BspPI GGATC 2 cut(s) 56, 552
BspT107I GGYRCC 1 cut(s) 83
BspTI CTTAAG 1 cut(s) 170
BsrDI GCAATG 2 cut(s) 68, 228
BsrI ACTGG 3 cut(s) 271, 337, 646
BssECI CCNNGG 3 cut(s) 555, 689, 812
BssMI GATC 5 cut(s) 48, 526, 544, 671, 702
Bst4CI ACNGT 1 cut(s) 791
BstAFI CTTAAG 1 cut(s) 170
BstC8I GCNNGC 4 cut(s) 75, 297, 445, 508
BstDEI CTNAG 2 cut(s) 32, 52
BstDSI CCRYGG 1 cut(s) 555
BstF5I GGATG 5 cut(s) 344, 419, 471, 603, 754
BstKTI GATC 5 cut(s) 51, 529, 547, 674, 705
BstMAI GTCTC 1 cut(s) 320
BstMBI GATC 5 cut(s) 48, 526, 544, 671, 702
BstMWI GCNNNNNNNGC 6 cut(s) 83, 144, 230, 308, 512, 758
BstSCI CCNGG 5 cut(s) 540, 561, 688, 689, 811
BstSFI CTRYAG 2 cut(s) 234, 778
BstSLI GKGCMC 2 cut(s) 95, 352
BstV1I GCAGC 4 cut(s) 220, 248, 286, 427
BstX2I RGATCY 2 cut(s) 48, 544
BstYI RGATCY 2 cut(s) 48, 544
BsuRI GGCC 2 cut(s) 554, 844
BtgI CCRYGG 1 cut(s) 555
BtsCI GGATG 5 cut(s) 344, 419, 471, 603, 754
BtsIMutI CAGTG 1 cut(s) 639
Cac8I GCNNGC 4 cut(s) 75, 297, 445, 508
CciI TCATGA 1 cut(s) 322
Cfr13I GGNCC 3 cut(s) 359, 467, 559
Cfr9I CCCGGG 1 cut(s) 689
Csp6I GTAC 1 cut(s) 787
CviAII CATG 5 cut(s) 203, 323, 369, 435, 629
CviQI GTAC 1 cut(s) 787
DdeI CTNAG 2 cut(s) 32, 52
DpnI GATC 5 cut(s) 50, 528, 546, 673, 704
DpnII GATC 5 cut(s) 48, 526, 544, 671, 702
EaeI YGGCCR 1 cut(s) 552
Eco147I AGGCCT 1 cut(s) 844
Eco47I GGWCC 3 cut(s) 359, 467, 559
Eco88I CYCGRG 1 cut(s) 689
EcoO109I RGGNCCY 1 cut(s) 467
EcoT22I ATGCAT 1 cut(s) 370
FaeI CATG 5 cut(s) 206, 326, 372, 438, 632
FaqI GGGAC 2 cut(s) 13, 830
FatI CATG 5 cut(s) 202, 322, 368, 434, 628
FauI CCCGC 3 cut(s) 499, 670, 728
FbaI TGATCA 1 cut(s) 702
Fnu4HI GCNGC 4 cut(s) 234, 237, 300, 441
FokI GGATG 5 cut(s) 351, 406, 458, 610, 761
Fsp4HI GCNGC 4 cut(s) 234, 237, 300, 441
FspBI CTAG 5 cut(s) 198, 548, 660, 753, 762
GluI GCNGC 4 cut(s) 234, 237, 300, 441
GsaI CCCAGC 1 cut(s) 98
GsuI CTGGAG 2 cut(s) 288, 354
HaeIII GGCC 2 cut(s) 554, 844
HapII CCGG 5 cut(s) 423, 542, 563, 690, 812
Hin1II CATG 5 cut(s) 206, 326, 372, 438, 632
HinfI GANTC 3 cut(s) 601, 652, 808
HpaII CCGG 5 cut(s) 423, 542, 563, 690, 812
HphI GGTGA 2 cut(s) 137, 697
Hpy166II GTNNAC 1 cut(s) 350
Hpy188I TCNGA 2 cut(s) 521, 531
Hpy188III TCNNGA 4 cut(s) 323, 577, 595, 746
Hpy8I GTNNAC 1 cut(s) 350
HpyAV CCTTC 2 cut(s) 733, 777
HpyCH4III ACNGT 1 cut(s) 791
HpyCH4V TGCA 6 cut(s) 10, 236, 350, 368, 414, 665
HpyF10VI GCNNNNNNNGC 6 cut(s) 83, 144, 230, 308, 512, 758
HpyF3I CTNAG 2 cut(s) 32, 52
Hsp92II CATG 5 cut(s) 206, 326, 372, 438, 632
Ksp22I TGATCA 1 cut(s) 702
Kzo9I GATC 5 cut(s) 48, 526, 544, 671, 702
LmnI GCTCC 1 cut(s) 292
Lsp1109I GCAGC 4 cut(s) 220, 248, 286, 427
LweI GCATC 3 cut(s) 182, 739, 777
MaeI CTAG 5 cut(s) 198, 548, 660, 753, 762
MaeIII GTNAC 2 cut(s) 125, 830
MalI GATC 5 cut(s) 50, 528, 546, 673, 704
MboI GATC 5 cut(s) 48, 526, 544, 671, 702
MflI RGATCY 2 cut(s) 48, 544
MhlI GDGCHC 2 cut(s) 95, 352
MlsI TGGCCA 1 cut(s) 554
MluNI TGGCCA 1 cut(s) 554
MlyI GAGTC 3 cut(s) 595, 661, 817
MmeI TCCRAC 2 cut(s) 131, 499
MnlI CCTC 6 cut(s) 63, 149, 328, 329, 458, 716
Mox20I TGGCCA 1 cut(s) 554
Mph1103I ATGCAT 1 cut(s) 370
MscI TGGCCA 1 cut(s) 554
MseI TTAA 3 cut(s) 171, 249, 276
Msp20I TGGCCA 1 cut(s) 554
MspA1I CMGCKG 1 cut(s) 677
MspCI CTTAAG 1 cut(s) 170
MspI CCGG 5 cut(s) 423, 542, 563, 690, 812
MspR9I CCNGG 5 cut(s) 542, 563, 690, 691, 813
Mva1269I GAATGC 1 cut(s) 737
MwoI GCNNNNNNNGC 6 cut(s) 83, 144, 230, 308, 512, 758
NciI CCSGG 5 cut(s) 542, 563, 690, 691, 813
NdeII GATC 5 cut(s) 48, 526, 544, 671, 702
NlaIII CATG 5 cut(s) 206, 326, 372, 438, 632
NlaIV GGNNCC 4 cut(s) 85, 469, 561, 687
NmuCI GTSAC 1 cut(s) 125
NsiI ATGCAT 1 cut(s) 370
PagI TCATGA 1 cut(s) 322
PceI AGGCCT 1 cut(s) 844
PctI GAATGC 1 cut(s) 737
PkrI GCNGC 4 cut(s) 235, 238, 301, 442
PleI GAGTC 3 cut(s) 595, 660, 816
PpsI GAGTC 3 cut(s) 595, 660, 816
PpuMI RGGWCCY 1 cut(s) 467
Psp5II RGGWCCY 1 cut(s) 467
PspFI CCCAGC 1 cut(s) 94
PspN4I GGNNCC 4 cut(s) 85, 469, 561, 687
PspPI GGNCC 3 cut(s) 359, 467, 559
PspPPI RGGWCCY 1 cut(s) 467
PstI CTGCAG 1 cut(s) 238
PsuI RGATCY 2 cut(s) 48, 544
RsaI GTAC 1 cut(s) 788
RsaNI GTAC 1 cut(s) 787
SaqAI TTAA 3 cut(s) 171, 249, 276
SatI GCNGC 4 cut(s) 234, 237, 300, 441
Sau3AI GATC 5 cut(s) 48, 526, 544, 671, 702
Sau96I GGNCC 3 cut(s) 359, 467, 559
SchI GAGTC 3 cut(s) 595, 661, 817
ScrFI CCNGG 5 cut(s) 542, 563, 690, 691, 813
SduI GDGCHC 2 cut(s) 95, 352
SfaNI GCATC 3 cut(s) 182, 739, 777
SfcI CTRYAG 2 cut(s) 234, 778
SinI GGWCC 3 cut(s) 359, 467, 559
SmaI CCCGGG 1 cut(s) 691
SmlI CTYRAG 1 cut(s) 170
SmoI CTYRAG 1 cut(s) 170
SseBI AGGCCT 1 cut(s) 844
SsiI CCGC 4 cut(s) 120, 506, 677, 735
SspMI CTAG 5 cut(s) 198, 548, 660, 753, 762
StuI AGGCCT 1 cut(s) 844
StyD4I CCNGG 5 cut(s) 540, 561, 688, 689, 811
TaaI ACNGT 1 cut(s) 791
TaqI TCGA 3 cut(s) 100, 159, 178
TaqII GACCGA 1 cut(s) 231
Tru1I TTAA 3 cut(s) 171, 249, 276
Tru9I TTAA 3 cut(s) 171, 249, 276
TscAI CASTG 1 cut(s) 646
TseFI GTSAC 1 cut(s) 125
TseI GCWGC 4 cut(s) 233, 236, 299, 440
Tsp45I GTSAC 1 cut(s) 125
TspDTI ATGAA 3 cut(s) 17, 217, 405
TspGWI ACGGA 2 cut(s) 572, 735
TspMI CCCGGG 1 cut(s) 689
TspRI CASTG 1 cut(s) 646
Vha464I CTTAAG 1 cut(s) 170
VneI GTGCAC 1 cut(s) 348
VpaK11BI GGWCC 3 cut(s) 359, 467, 559
XmaI CCCGGG 1 cut(s) 689
XspI CTAG 5 cut(s) 198, 548, 660, 753, 762
Zsp2I ATGCAT 1 cut(s) 370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.