FvH4_4g15020

Carboxylesterase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000306
Physical Location & Seq
Reverse (-)
18612699 .. 18614651
1953 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g15020.t1

Sequence Viewer

Length: 1953 bp
ATGGGTTCCCTTGCCAAGGAGATAGACAGAGAGGTGATCCCATTTGTCCGTGTTTACAAAGATGGCTCAGTTGAAAGACTACTGGGTACTCCTTACAAAGCACCATTGCTCAATGATCCTGTATCTGGAGTCTCATCCAAAGATGTCACCATTTCTAAAGTCCCTTTGATCTCAGCCAGAATCTTCCTCCCTAAACTCGATGATGAGCAGCCTCAACAGAAACTCCCCATCTTGGTTTACTGCCATGGTGGAGGTTTCTGTATCGAATCCGCCTTCTCTTTCGATCACCACCGGTTTCTTGACGGCTTGGTGTCCCAAGCTAGAGTTGTTGCTGTTTCTGTGGAGTACAGGATGGCTCCTGAACACCCTCTTCCTGCAGGCTATGAAGACTGTTGGGCTGCACTGGAATGGGTGGCTTCACACTTCGTTGAAAATGGCATCAGTGAGGAGCCTTGGTTGACGAATCACGGCAACCCGGAGTATTTCTTTCTTGGCGGTGATAGTGCAGGAGCTAACATTGCTCATAATGTGGCAATGAGAGTTGGCAAAGAGGGCTTGCCTGGTGGTTTGAAGATACTGGGGCTCTCTCTTACTCACCCCTATTTTTGGGGTTCCAAGCCTATTGCGGAAGACCCTTGCAAAGAGCCTGAGAAGGATTTGGCAGCCATGCTTTGGAACTTTGGGTACCCTTCAGCACCTGGAGGGCTTGATAATCCATTCATCAATCCTCTTGCTCCGGAAGCACCATGCTTGGCAGGACTAGGGTGTTCCAAGTTGTTTGTGAGCGTCTCGGAACATGATGGGCTGAAGTTTCGAGGCATTCGATACTACGATGCCGTGAAGAAAAGTGGGTGGAAAGGAGAGGCAGAGCTGGTGGACGTGGAAGGAGAGGCTCATGCATTTCATATTTTGAAATTTGAGACTCAGAAAGCCCAGGATTTGATAAAAAAGTTGGTTGATTTTCTTAAGGTGGAAGGCAAGAAGGTAGAGAAGGAGGTAGAAAAAGAGCTAATACCATTTGTCCGAGTCTACAAGGATGGCTCGGTTGAACGACTCATGGGCACTCCATATGTGCCCCCAATGCTTAATGATCCAGTATCAGGAGTATCATCAAAAGATATCACAATTTCGCAAAATCCGTTGATCTCGGCCAGAGTCTTCCTCCCAAAACTTGACGCTGATCAACCACACCAGAAGCTCCCCATCTTGGTTTACTTCCATGGAGGAGCTTTTATGATCGAATCCGCATTCTCATTCGATCACCACAGGTATCTTGACAGCTTGGTTTCCCAAGCCAAAGTAGTTGCTGTTTCAGTTGAGTACAGGATGGCTCCTGAGCACCCTCTTCCTGCTGGCTATGAAGATTGTTGGGCTGCCCTCCAATGGGTTGCCTCACACTTCGTTGATAATGGTATCAGTGAAGAGCCTTGGTTGACAAATCATGGCAACCCGGACAGGTTTTTTCTTGGTGGTGATAGTGCAGGTGCCAACATTGCTCATAATGTGGCAATGAGAGTTGGGAAAGAAGGTTTGCCTGGTGGTCTAAAAATAAGGGGAACCTCCCTCACACACCCTTATTTTTGGGGTTCCAAGCCACTCGGGGAATCAGACCCTTGCAAAGAGCCTGAGAAGGATTTGGGGTGTAATGTTTGGAACTTCTCGTACCCTAATGCGCCTGGTGGGATTGATAATCCAATGATGAATCCGTTTGGTCCAGAAGCACCAAGCTTGGCTGGACTTGGGTGTTCAAAGTTGTTTGTTAGTGTTTCTGAGAAGGATACTCTGAGATATAGAGGTGTTCATTACTATGAGGCAGTGAAGAAAAGTGGTTGGAAAGGTGAAGCAGAGTTGGTTGAAGTGGTCGGAGAAGAACATGCGTTTCATATTTTGAAGTTTGAGACTCCGAAAGCTCAGGACCTGACTAGAAAAGTGGCTGAGTTTCTCCTCGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

651

Amino Acids

71.65

Weight (kDa)

5.63

Isoelectric Point (pI)

28.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 61 - 179 3.3e-08 Carboxylesterase family
BD-FAE PF20434 70 - 181 8e-09 BD-FAE
Abhydrolase_3 PF07859 78 - 302 1.9e-44 alpha/beta hydrolase fold
COesterase PF00135 386 - 548 4.4e-10 Carboxylesterase family
BD-FAE PF20434 396 - 508 8.2e-10 BD-FAE
Abhydrolase_3 PF07859 403 - 628 6.2e-46 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000306)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19330
fragaria_vesca FvH4_2g23530 FvH4_2g23530 FvH4_2g23530 FvH4_2g23530 FvH4_2g23530 FvH4_2g23530 FvH4_4g15010 FvH4_4g15020 FvH4_4g15040 FvH4_4g15040 FvH4_4g15040 FvH4_4g15040 FvH4_4g15040 FvH4_4g15040
malus_domestica MD03G1264200.v1.1 MD03G1264700.v1.1 MD11G1284800.v1.1 MD13G1187400.v1.1 MD13G1187500.v1.1 MD13G1187900.v1.1 MD13G1188000.v1.1 MD16G1188100.v1.1 MD16G1188500.v1.1 MD16G1188600.v1.1
prunus_persica Prupe.1G154900_v2.0.a1 Prupe.1G155100_v2.0.a1 Prupe.1G155200_v2.0.a1 Prupe.1G155300_v2.0.a1 Prupe.1G155300_v2.0.a1 Prupe.8G240700_v2.0.a1
pyrus_communis pycom03g21190 pycom11g25240 pycom11g25250 pycom13g16210 pycom13g16230 pycom13g16240 pycom16g15820
rosa_chinensis RchiOBHm_Chr1g0343661 RchiOBHm_Chr4g0416821 RchiOBHm_Chr4g0416831 RchiOBHm_Chr4g0416841 RchiOBHm_Chr4g0416871 RchiOBHm_Chr4g0416951 RchiOBHm_Chr4g0416961 RchiOBHm_Chr6g0291001
rosa_laevigata RLG00000007939 RLG00000007940 RLG00000007944 RLG00000007945 RLG00000007946 RLG00000007954 RLG00000012156 RLG00000028955
rosa_multiflora Rmu_co8241865.1_g000001 Rmu_sc0000021.1_g000004 Rmu_sc0000663.1_g000003 Rmu_sc0000663.1_g000009 Rmu_sc0000663.1_g000012 Rmu_sc0000663.1_g000013 Rmu_sc0000663.1_g000021 Rmu_sc0000663.1_g000029 Rmu_sc0014328.1_g000002 Rmu_sc0014328.1_g000003 Rmu_sc0020688.1_g000001 Rmu_sc0024683.1_g000001 Rmu_sc0024683.1_g000002 Rmu_sc0033477.1_g000001 Rmu_sc0035872.1_g000001 Rmu_ssc0000010.1_g000006 Rmu_ssc0000010.1_g000008 Rmu_ssc0000010.1_g000011
rosa_roxburghii Rroxscaffold_4G00310390 Rroxscaffold_5G00360700 Rroxscaffold_5G00360710 Rroxscaffold_5G00360720 Rroxscaffold_5G00360750 Rroxscaffold_5G00360800 Rroxscaffold_5G00360820 Rroxscaffold_7G00176470
rosa_rugosa Rorug04G0145200 Rorug04G0145300 Rorug04G0145400 Rorug04G0145400 Rorug04G0145600.1 Rorug04G0145700.1 Rorug04G0148500 Rorug06G0217000
rosa_samantha Rh2BG232000 Rh4AG207900 Rh4BG205000 Rh4BG205100 Rh4CG218700 Rh4DG134800 Rh4DG204800 Rh4DG204900 Rh4DG205000 Rh4DG205100 Rh4DG205200 Rh4DG205300 Rh4DG205400 Rh4DG205500 Rh5BG434500 Rh5CG457700 Rh6AG326800 Rh6BG334300 Rh6CG340500 Rh6DG327800
rosa_wichuraiana Rw1G015270 Rw4G017570 Rw4G017580 Rw4G017590 Rw4G017600 Rw4G017610 Rw4G017620 Rw4G017630 Rw4G017640 Rw6G028420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1472
Acc36I ACCTGC 1 cut(s) 1472
Acc65I GGTACC 1 cut(s) 684
AccB1I GGYRCC 2 cut(s) 684, 1484
AccB7I CCANNNNNTGG 1 cut(s) 672
AccI GTMKAC 1 cut(s) 1029
AccIII TCCGGA 1 cut(s) 736
AciI CCGC 4 cut(s) 270, 495, 626, 1245
AclWI GGATC 3 cut(s) 31, 110, 1085
AcoI YGGCCR 1 cut(s) 1149
AcsI RAATTY 1 cut(s) 914
AcuI CTGAAG 2 cut(s) 675, 827
AfaI GTAC 5 cut(s) 88, 347, 686, 1322, 1664
AfiI CCNNNNNNNGG 9 cut(s) 16, 125, 232, 606, 672, 1100, 1207, 1383, 1384
AflII CTTAAG 1 cut(s) 965
AgeI ACCGGT 1 cut(s) 291
AgsI TTSAA 8 cut(s) 74, 431, 571, 913, 1049, 1749, 1856, 1891
AjiI CACGTC 1 cut(s) 880
AjnI CCWGG 5 cut(s) 559, 697, 933, 1534, 1675
AluBI AGCT 9 cut(s) 320, 512, 871, 1009, 1198, 1229, 1281, 1728, 1910
AluI AGCT 9 cut(s) 320, 512, 871, 1009, 1198, 1229, 1281, 1728, 1910
Alw21I GWGCWC 1 cut(s) 1341
Alw26I GTCTC 4 cut(s) 136, 793, 914, 1892
AlwI GGATC 3 cut(s) 31, 110, 1085
AlwNI CAGNNNCTG 2 cut(s) 698, 1918
Ama87I CYCGRG 2 cut(s) 1598, 1946
Aor13HI TCCGGA 1 cut(s) 736
AoxI GGCC 1 cut(s) 1149
ApeKI GCWGC 4 cut(s) 208, 398, 662, 1373
ApoI RAATTY 1 cut(s) 914
AsiGI ACCGGT 1 cut(s) 291
Asp718I GGTACC 1 cut(s) 684
AspLEI GCGC 1 cut(s) 1675
AspS9I GGNCC 2 cut(s) 1712, 1915
AsuC2I CCSGG 2 cut(s) 476, 1451
AsuHPI GGTGA 8 cut(s) 46, 139, 278, 509, 587, 1253, 1484, 1850
AvaI CYCGRG 2 cut(s) 1598, 1946
AvaII GGWCC 2 cut(s) 1712, 1915
BaeGI GKGCMC 2 cut(s) 1064, 1077
BanI GGYRCC 2 cut(s) 684, 1484
BanII GRGCYC 1 cut(s) 585
BbsI GAAGAC 3 cut(s) 393, 636, 1150
Bbv12I GWGCWC 1 cut(s) 1341
BbvI GCAGC 4 cut(s) 220, 385, 674, 1360
BccI CCATC 7 cut(s) 56, 236, 346, 794, 1031, 1211, 1321
BceAI ACGGC 3 cut(s) 319, 484, 821
BciT130I CCWGG 5 cut(s) 561, 699, 935, 1536, 1677
BciVI GTATCC 1 cut(s) 1771
BclI TGATCA 1 cut(s) 1180
BcnI CCSGG 2 cut(s) 476, 1451
BcoDI GTCTC 4 cut(s) 136, 793, 914, 1892
BfaI CTAG 3 cut(s) 321, 761, 1923
BfmI CTRYAG 1 cut(s) 375
BfrI CTTAAG 1 cut(s) 965
BfuAI ACCTGC 1 cut(s) 1472
BfuI GTATCC 1 cut(s) 1771
BisI GCNGC 4 cut(s) 209, 399, 663, 1374
BlsI GCNGC 4 cut(s) 210, 400, 664, 1375
Bme1390I CCNGG 7 cut(s) 476, 561, 699, 935, 1451, 1536, 1677
Bme18I GGWCC 2 cut(s) 1712, 1915
BmeT110I CYCGRG 2 cut(s) 1598, 1946
BmgBI CACGTC 1 cut(s) 880
BmgT120I GGNCC 2 cut(s) 1712, 1915
BmiI GGNNCC 9 cut(s) 7, 357, 450, 613, 686, 1332, 1486, 1558, 1588
BmrFI CCNGG 7 cut(s) 476, 561, 699, 935, 1451, 1536, 1677
BmrI ACTGGG 2 cut(s) 92, 587
BmsI GCATC 2 cut(s) 447, 823
BmuI ACTGGG 2 cut(s) 92, 587
BpiI GAAGAC 3 cut(s) 393, 636, 1150
BplI GAGNNNNNCTC 2 cut(s) 1146, 1178
BpmI CTGGAG 2 cut(s) 147, 720
Bpu10I CCTNAGC 2 cut(s) 1335, 1911
BpuMI CCSGG 2 cut(s) 476, 1451
BsaJI CCNNGG 6 cut(s) 15, 244, 452, 933, 1219, 1427
BsaWI WCCGGW 2 cut(s) 291, 736
BsaXI ACNNNNNCTCC 4 cut(s) 207, 237, 440, 470
Bsc4I CCNNNNNNNGG 9 cut(s) 16, 125, 232, 606, 672, 1100, 1207, 1383, 1384
Bse118I RCCGGY 1 cut(s) 291
Bse1I ACTGG 4 cut(s) 87, 408, 582, 1094
Bse3DI GCAATG 5 cut(s) 104, 516, 540, 1491, 1515
BseAI TCCGGA 1 cut(s) 736
BseBI CCWGG 5 cut(s) 561, 699, 935, 1536, 1677
BseDI CCNNGG 6 cut(s) 15, 244, 452, 933, 1219, 1427
BseGI GGATG 4 cut(s) 134, 357, 1042, 1332
BseLI CCNNNNNNNGG 9 cut(s) 16, 125, 232, 606, 672, 1100, 1207, 1383, 1384
BseMI GCAATG 5 cut(s) 104, 516, 540, 1491, 1515
BseNI ACTGG 4 cut(s) 87, 408, 582, 1094
BseRI GAGGAG 3 cut(s) 461, 1239, 1934
BseSI GKGCMC 2 cut(s) 1064, 1077
BseXI GCAGC 4 cut(s) 220, 385, 674, 1360
BsgI GTGCAG 3 cut(s) 384, 525, 1500
BshFI GGCC 1 cut(s) 1151
BshNI GGYRCC 2 cut(s) 684, 1484
BshTI ACCGGT 1 cut(s) 291
BsiHKAI GWGCWC 1 cut(s) 1341
BsiHKCI CYCGRG 2 cut(s) 1598, 1946
BsiSI CCGG 4 cut(s) 292, 476, 737, 1451
BslFI GGGAC 2 cut(s) 146, 298
BslI CCNNNNNNNGG 9 cut(s) 16, 125, 232, 606, 672, 1100, 1207, 1383, 1384
BsmAI GTCTC 4 cut(s) 136, 793, 914, 1892
BsmBI CGTCTC 1 cut(s) 793
BsmFI GGGAC 2 cut(s) 146, 298
BsmI GAATGC 2 cut(s) 819, 1247
BsnI GGCC 1 cut(s) 1151
BsoBI CYCGRG 2 cut(s) 1598, 1946
Bsp1286I GDGCHC 4 cut(s) 585, 1064, 1077, 1341
Bsp13I TCCGGA 1 cut(s) 736
Bsp143I GATC 9 cut(s) 36, 115, 168, 283, 1090, 1143, 1180, 1236, 1258
Bsp19I CCATGG 2 cut(s) 244, 1219
BspACI CCGC 4 cut(s) 270, 495, 626, 1245
BspANI GGCC 1 cut(s) 1151
BspEI TCCGGA 1 cut(s) 736
BspLI GGNNCC 9 cut(s) 7, 357, 450, 613, 686, 1332, 1486, 1558, 1588
BspMAI CTGCAG 1 cut(s) 379
BspMI ACCTGC 1 cut(s) 1472
BspPI GGATC 3 cut(s) 31, 110, 1085
BspQI GCTCTTC 1 cut(s) 1416
BspT107I GGYRCC 2 cut(s) 684, 1484
BspTI CTTAAG 1 cut(s) 965
BsrDI GCAATG 5 cut(s) 104, 516, 540, 1491, 1515
BsrFI RCCGGY 1 cut(s) 291
BsrI ACTGG 4 cut(s) 87, 408, 582, 1094
BssAI RCCGGY 1 cut(s) 291
BssECI CCNNGG 6 cut(s) 15, 244, 452, 933, 1219, 1427
BssMI GATC 9 cut(s) 36, 115, 168, 283, 1090, 1143, 1180, 1236, 1258
BssT1I CCWWGG 5 cut(s) 15, 244, 452, 1219, 1427
Bst2UI CCWGG 5 cut(s) 561, 699, 935, 1536, 1677
Bst4CI ACNGT 1 cut(s) 392
Bst6I CTCTTC 3 cut(s) 375, 1350, 1416
BstAFI CTTAAG 1 cut(s) 965
BstC8I GCNNGC 3 cut(s) 379, 557, 1354
BstDSI CCRYGG 2 cut(s) 244, 1219
BstENI CCTNNNNNAGG 1 cut(s) 14
BstF5I GGATG 4 cut(s) 134, 357, 1042, 1332
BstHHI GCGC 1 cut(s) 1675
BstKTI GATC 9 cut(s) 39, 118, 171, 286, 1093, 1146, 1183, 1239, 1261
BstMAI GTCTC 4 cut(s) 136, 793, 914, 1892
BstMBI GATC 9 cut(s) 36, 115, 168, 283, 1090, 1143, 1180, 1236, 1258
BstMWI GCNNNNNNNGC 5 cut(s) 518, 552, 740, 1081, 1493
BstNI CCWGG 5 cut(s) 561, 699, 935, 1536, 1677
BstNSI RCATGY 1 cut(s) 1877
BstSCI CCNGG 7 cut(s) 474, 559, 697, 933, 1449, 1534, 1675
BstSFI CTRYAG 1 cut(s) 375
BstSLI GKGCMC 2 cut(s) 1064, 1077
BstV1I GCAGC 4 cut(s) 220, 385, 674, 1360
BstV2I GAAGAC 3 cut(s) 393, 636, 1150
BsuI GTATCC 1 cut(s) 1771
BsuRI GGCC 1 cut(s) 1151
BtgI CCRYGG 2 cut(s) 244, 1219
BtrI CACGTC 1 cut(s) 880
BtsCI GGATG 4 cut(s) 134, 357, 1042, 1332
BtsI GCAGTG 1 cut(s) 1821
BtsIMutI CAGTG 4 cut(s) 401, 448, 1423, 1821
BveI ACCTGC 1 cut(s) 1472
Cac8I GCNNGC 3 cut(s) 379, 557, 1354
CaiI CAGNNNCTG 2 cut(s) 698, 1918
CfoI GCGC 1 cut(s) 1675
Cfr10I RCCGGY 1 cut(s) 291
Cfr13I GGNCC 2 cut(s) 1712, 1915
CseI GACGC 2 cut(s) 775, 1184
Csp6I GTAC 5 cut(s) 87, 346, 685, 1321, 1663
CspAI ACCGGT 1 cut(s) 291
CviAII CATG 9 cut(s) 245, 667, 747, 797, 896, 1057, 1220, 1442, 1874
CviQI GTAC 5 cut(s) 87, 346, 685, 1321, 1663
DpnI GATC 9 cut(s) 38, 117, 170, 285, 1092, 1145, 1182, 1238, 1260
DpnII GATC 9 cut(s) 36, 115, 168, 283, 1090, 1143, 1180, 1236, 1258
EaeI YGGCCR 1 cut(s) 1149
Eam1104I CTCTTC 3 cut(s) 375, 1350, 1416
EarI CTCTTC 3 cut(s) 375, 1350, 1416
EciI GGCGGA 1 cut(s) 259
Eco130I CCWWGG 5 cut(s) 15, 244, 452, 1219, 1427
Eco24I GRGCYC 1 cut(s) 585
Eco32I GATATC 1 cut(s) 1120
Eco47I GGWCC 2 cut(s) 1712, 1915
Eco57I CTGAAG 2 cut(s) 675, 827
Eco88I CYCGRG 2 cut(s) 1598, 1946
EcoNI CCTNNNNNAGG 1 cut(s) 14
EcoO109I RGGNCCY 1 cut(s) 1915
EcoRII CCWGG 5 cut(s) 559, 697, 933, 1534, 1675
EcoRV GATATC 1 cut(s) 1120
EcoT14I CCWWGG 5 cut(s) 15, 244, 452, 1219, 1427
EcoT22I ATGCAT 1 cut(s) 901
EcoT38I GRGCYC 1 cut(s) 585
ErhI CCWWGG 5 cut(s) 15, 244, 452, 1219, 1427
Esp3I CGTCTC 1 cut(s) 793
FaeI CATG 9 cut(s) 248, 670, 750, 800, 899, 1060, 1223, 1445, 1877
FaqI GGGAC 2 cut(s) 146, 298
FatI CATG 9 cut(s) 244, 666, 746, 796, 895, 1056, 1219, 1441, 1873
FauNDI CATATG 1 cut(s) 1069
FbaI TGATCA 1 cut(s) 1180
FblI GTMKAC 1 cut(s) 1029
Fnu4HI GCNGC 4 cut(s) 209, 399, 663, 1374
FokI GGATG 4 cut(s) 121, 364, 1049, 1339
FriOI GRGCYC 1 cut(s) 585
Fsp4HI GCNGC 4 cut(s) 209, 399, 663, 1374
FspBI CTAG 3 cut(s) 321, 761, 1923
GlaI GCGC 1 cut(s) 1674
GluI GCNGC 4 cut(s) 209, 399, 663, 1374
GsuI CTGGAG 2 cut(s) 147, 720
HaeIII GGCC 1 cut(s) 1151
HapII CCGG 4 cut(s) 292, 476, 737, 1451
HgaI GACGC 2 cut(s) 775, 1184
HhaI GCGC 1 cut(s) 1675
Hin1II CATG 9 cut(s) 248, 670, 750, 800, 899, 1060, 1223, 1445, 1877
Hin6I GCGC 1 cut(s) 1673
HinP1I GCGC 1 cut(s) 1673
HincII GTYRAC 2 cut(s) 459, 1434
HindII GTYRAC 2 cut(s) 459, 1434
HindIII AAGCTT 1 cut(s) 1726
HpaII CCGG 4 cut(s) 292, 476, 737, 1451
HphI GGTGA 8 cut(s) 46, 139, 278, 509, 587, 1253, 1484, 1850
Hpy166II GTNNAC 7 cut(s) 55, 238, 459, 877, 1030, 1213, 1434
Hpy188I TCNGA 8 cut(s) 793, 927, 1025, 1609, 1771, 1785, 1865, 1905
Hpy188III TCNNGA 9 cut(s) 126, 299, 359, 737, 1101, 1274, 1334, 1715, 1913
Hpy8I GTNNAC 7 cut(s) 55, 238, 459, 877, 1030, 1213, 1434
HpyCH4III ACNGT 1 cut(s) 392
HpyCH4IV ACGT 1 cut(s) 879
HpyCH4V TGCA 7 cut(s) 377, 401, 506, 639, 899, 1481, 1617
HpyF10VI GCNNNNNNNGC 5 cut(s) 518, 552, 740, 1081, 1493
HpySE526I ACGT 1 cut(s) 879
Hsp92II CATG 9 cut(s) 248, 670, 750, 800, 899, 1060, 1223, 1445, 1877
HspAI GCGC 1 cut(s) 1673
Kpn2I TCCGGA 1 cut(s) 736
KpnI GGTACC 1 cut(s) 688
Ksp22I TGATCA 1 cut(s) 1180
Kzo9I GATC 9 cut(s) 36, 115, 168, 283, 1090, 1143, 1180, 1236, 1258
LguI GCTCTTC 1 cut(s) 1416
LmnI GCTCC 7 cut(s) 361, 448, 509, 739, 1203, 1226, 1336
Lsp1109I GCAGC 4 cut(s) 220, 385, 674, 1360
LweI GCATC 2 cut(s) 447, 823
MaeI CTAG 3 cut(s) 321, 761, 1923
MaeII ACGT 1 cut(s) 879
MaeIII GTNAC 1 cut(s) 145
MalI GATC 9 cut(s) 38, 117, 170, 285, 1092, 1145, 1182, 1238, 1260
MboI GATC 9 cut(s) 36, 115, 168, 283, 1090, 1143, 1180, 1236, 1258
MhlI GDGCHC 4 cut(s) 585, 1064, 1077, 1341
MluCI AATT 2 cut(s) 914, 1125
MlyI GAGTC 6 cut(s) 138, 916, 1035, 1047, 1164, 1894
MmeI TCCRAC 2 cut(s) 1811, 1843
Mph1103I ATGCAT 1 cut(s) 901
MroI TCCGGA 1 cut(s) 736
MseI TTAA 2 cut(s) 966, 1086
MslI CAYNNNNRTG 2 cut(s) 406, 1806
MspCI CTTAAG 1 cut(s) 965
MspI CCGG 4 cut(s) 292, 476, 737, 1451
MspR9I CCNGG 7 cut(s) 476, 561, 699, 935, 1451, 1536, 1677
Mva1269I GAATGC 2 cut(s) 819, 1247
MvaI CCWGG 5 cut(s) 561, 699, 935, 1536, 1677
MwoI GCNNNNNNNGC 5 cut(s) 518, 552, 740, 1081, 1493
NciI CCSGG 2 cut(s) 476, 1451
NcoI CCATGG 2 cut(s) 244, 1219
NdeI CATATG 1 cut(s) 1069
NdeII GATC 9 cut(s) 36, 115, 168, 283, 1090, 1143, 1180, 1236, 1258
NlaIII CATG 9 cut(s) 248, 670, 750, 800, 899, 1060, 1223, 1445, 1877
NlaIV GGNNCC 9 cut(s) 7, 357, 450, 613, 686, 1332, 1486, 1558, 1588
NmeAIII GCCGAG 1 cut(s) 1127
NmuCI GTSAC 1 cut(s) 145
NsiI ATGCAT 1 cut(s) 901
NspI RCATGY 1 cut(s) 1877
PaeR7I CTCGAG 1 cut(s) 1946
PaqCI CACCTGC 1 cut(s) 1472
PciSI GCTCTTC 1 cut(s) 1416
PcsI WCGNNNNNNNCGW 2 cut(s) 820, 1136
PctI GAATGC 2 cut(s) 819, 1247
PfeI GAWTC 6 cut(s) 180, 266, 463, 1241, 1604, 1702
PflMI CCANNNNNTGG 1 cut(s) 672
PinAI ACCGGT 1 cut(s) 291
PkrI GCNGC 4 cut(s) 210, 400, 664, 1375
PleI GAGTC 6 cut(s) 137, 916, 1034, 1047, 1163, 1894
PpsI GAGTC 6 cut(s) 137, 916, 1034, 1047, 1163, 1894
PpuMI RGGWCCY 1 cut(s) 1915
Psp5II RGGWCCY 1 cut(s) 1915
Psp6I CCWGG 5 cut(s) 559, 697, 933, 1534, 1675
PspGI CCWGG 5 cut(s) 559, 697, 933, 1534, 1675
PspN4I GGNNCC 9 cut(s) 7, 357, 450, 613, 686, 1332, 1486, 1558, 1588
PspPI GGNCC 2 cut(s) 1712, 1915
PspPPI RGGWCCY 1 cut(s) 1915
PspXI VCTCGAGB 1 cut(s) 1946
PsrI GAACNNNNNNTAC 4 cut(s) 668, 700, 1646, 1678
PstI CTGCAG 1 cut(s) 379
PstNI CAGNNNCTG 2 cut(s) 698, 1918
RsaI GTAC 5 cut(s) 88, 347, 686, 1322, 1664
RsaNI GTAC 5 cut(s) 87, 346, 685, 1321, 1663
RseI CAYNNNNRTG 2 cut(s) 406, 1806
SapI GCTCTTC 1 cut(s) 1416
SaqAI TTAA 2 cut(s) 966, 1086
SatI GCNGC 4 cut(s) 209, 399, 663, 1374
Sau3AI GATC 9 cut(s) 36, 115, 168, 283, 1090, 1143, 1180, 1236, 1258
Sau96I GGNCC 2 cut(s) 1712, 1915
SbfI CCTGCAGG 1 cut(s) 379
SchI GAGTC 6 cut(s) 138, 916, 1035, 1047, 1164, 1894
ScrFI CCNGG 7 cut(s) 476, 561, 699, 935, 1451, 1536, 1677
SdaI CCTGCAGG 1 cut(s) 379
SduI GDGCHC 4 cut(s) 585, 1064, 1077, 1341
SfaNI GCATC 2 cut(s) 447, 823
SfcI CTRYAG 1 cut(s) 375
Sfr274I CTCGAG 1 cut(s) 1946
SinI GGWCC 2 cut(s) 1712, 1915
SlaI CTCGAG 1 cut(s) 1946
SmiMI CAYNNNNRTG 2 cut(s) 406, 1806
SmlI CTYRAG 2 cut(s) 965, 1946
SmoI CTYRAG 2 cut(s) 965, 1946
Sse8387I CCTGCAGG 1 cut(s) 379
Sse9I AATT 2 cut(s) 914, 1125
SsiI CCGC 4 cut(s) 270, 495, 626, 1245
SspMI CTAG 3 cut(s) 321, 761, 1923
StyD4I CCNGG 7 cut(s) 474, 559, 697, 933, 1449, 1534, 1675
StyI CCWWGG 5 cut(s) 15, 244, 452, 1219, 1427
TaaI ACNGT 1 cut(s) 392
TaiI ACGT 1 cut(s) 882
TaqI TCGA 8 cut(s) 198, 264, 282, 814, 823, 1239, 1257, 1947
TasI AATT 2 cut(s) 914, 1125
TatI WGTACW 2 cut(s) 345, 1320
TfiI GAWTC 6 cut(s) 180, 266, 463, 1241, 1604, 1702
Tru1I TTAA 2 cut(s) 966, 1086
Tru9I TTAA 2 cut(s) 966, 1086
TscAI CASTG 4 cut(s) 408, 448, 1423, 1821
TseFI GTSAC 1 cut(s) 145
TseI GCWGC 4 cut(s) 208, 398, 662, 1373
Tsp45I GTSAC 1 cut(s) 145
TspDTI ATGAA 7 cut(s) 399, 709, 893, 1374, 1715, 1790, 1871
TspGWI ACGGA 3 cut(s) 38, 1128, 1695
TspRI CASTG 4 cut(s) 408, 448, 1423, 1821
Van91I CCANNNNNTGG 1 cut(s) 672
Vha464I CTTAAG 1 cut(s) 965
VpaK11BI GGWCC 2 cut(s) 1712, 1915
XagI CCTNNNNNAGG 1 cut(s) 14
XapI RAATTY 1 cut(s) 914
XceI RCATGY 1 cut(s) 1877
XhoI CTCGAG 1 cut(s) 1946
XmiI GTMKAC 1 cut(s) 1029
XspI CTAG 3 cut(s) 321, 761, 1923
Zsp2I ATGCAT 1 cut(s) 901
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.