Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0000306

Physical Location & Seq

Genomic Coordinates Forward (+)

34988259 .. 34993275

Gene Structure 5017 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1264200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2148 bp

ATGGAACGCTCTCGGACGCGAGCCGTCACTACCAAAAGAGGCTTGAAGCGGAAGCTCGAAGAAGACTTCAGAGAAGAAAGGGTTGAGGGTGAACCGGACCGGAAAGTCCCGCCCGTCTTCGAACAGCAAGACGATCACCCAGATCTCGTCGTTCGGGTTCGCGAGCAGGTTCAGATTCTGGAGTCCAAATTCTCGTCTGCCCAATCCGACCGCCTACTCGCCAAGCAGGCCGCCCATGGCCTCGCTCTCACCGCCAAGGAAGAGGAGCTCGTGGACATCTTGGTGGACTGTGGAGCTGTTCCGGCTCTGGTTAAGCATCTGCGGGCGCCGGCGGCTGACGGCCGAAACTGTCCGATCCCTTACGAACACGAGGTCGAGAAATACTGTGCTTATGCCCTGGGACTTATTGTCATTAAGCTGGAGTATCAACAACTCGTGGTTGATGCCGGAGCTTTACCACATCTTGTTGATCTGTTAAAAAGGCACAAGCGGGGAAGTGAATCACCGGCACCTGATGCTGTGATCAGGAGAGCTTGTGATACAATTGCTCACATTGCCCATGAAAATGGCAACACTAAGAACCTTGTCAGGATTGAAGGTGGAATCCCTCCTCTTGTTGAACTGCTTAACTTCTTCAACACAAAGGTACAGATAGCGGCTGCAGGTGCCCTTCGGACTCTGGCATTTAAAAATGATGAAAACAAAAACCAGATTGTAGTAGAGTGCGGTGCTTTGCCTACCCTGGTACTAATGCTTCAATCTGAGGATCCTGTGATGTCCTTTGAAGCGGTTGGTGTAATAGGCAATCTTGTCCACTCATCCCCACACATCAAGAAATTAGTACTTCCTGCTGGTGCTTTACAACCTATTATTGGATTGCTCAGTTCCGACTGTACGGAGAGCCAAAGAGAAGCTGCTTTATTACTTGGTCAGTTTGCTGCAACTGATACAGATTGCAAGGCACATATTGTTCAGAGGGGTGCTTTAAAACCATTAATTGAGATGCTTAAGTCACCTGATGCACAGGTTAAGGAGATGTCTGCCTTTACGCTTGGGAGGTTGGCACAGGAAATACATAACCAAGCTGGCATTGCTCAGAATGGTGGTATCAAGCCTCTAATCGAGCTTCTTGGTTCAGAAAACATATCTCTCCAACATAATGCTGCGTTTTGTCTATATGGTCTTGCAGATAATGAGGATAACACTGCAACTATAATTAAGTTTGGAGGTGTTCAGAAACTTCAAGATGGAGAATTCATTGCTCAACAAACTAGGGAATGCGTGGCAAAGTCATTGAAAAGACTAGAGGAGAAGATTTGTGGACGAGTTTTAAACCATCTGTTATATATGATGCGGGTTGAAGATAAGGCCACTCAAAGAAGAGTGGCTTTAGCTCTTGCTCATCTTTGCAACCCTAGTGACAGGGAAAACATTTTTATTTCTAACAAAGGATTAGAGTTGCTCTTAGGGCTTCTTAATTCTCCACGGTATAAGGAGAAACATGACGGTTCTTTGGCCTTGTACAAACTGGCTACAAAAGCTACTTCACTCTCTCGTGTGGATGCAGCTCCTCCATCACCCTCCCCACAGGTGTATTTGGGTGAGCAATACGTAAACAATCCTACCTTTTCGGACATCACATTCTTAGTTGAAGGTAAACGGTTCTATGCCCACAGAATTTGTTTGCTTGCCTCATCTGATGCATTCCGTGCCATGTTTGATGGTGGTTACAGGGAGAGGGATGCAAGGGACGTTGTGATTCCAAATATTAGATGGGAAGTTTTTGAATTGATGATGAGATTCATATACACGGGATCGGTAGATGTCAATATGGATATTGCTCAGGATCTCCTCAGAGCTGCTGATCAGTATCTTTTGGAGGGCCTCAAACGGCTCTGCGAGCATGCCATTGTTCAGGACATCTGCGTGGAAAATCTTTCGCTCATGTTTGAGTTTTCGGAGGCCTTAAATGCCATGTCGTTACAGCAAGCATGCATTCTATTTCTACTGGAGCAATTTGATAAATTGGTTGACAAACCATGGTATCCCGCATTGGTGGATCGTGTGATATCAGACATTCGCGCATTTTTTACTACTGCACTTCAGATGCCCGTTGAGGCAACCTCGCAGCAGCAGGCAATGGCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006275 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008156 GO:0008285 GO:0009058 GO:0009059 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010556 GO:0010558 GO:0010605 GO:0019219 GO:0019222 GO:0031261 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032991 GO:0032993 GO:0034641 GO:0034645 GO:0042127 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051052 GO:0051053 GO:0051171 GO:0051172 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0080090 GO:0090304 GO:0090329 GO:1901360 GO:1901576 GO:2000104 GO:2000112 GO:2000113

KEGG Pathways

Metabolic & Signaling

ko04340 ko04341 map04340 map04341

Pfam Domains

Protein Families

Arm BTB

Protein Analysis

716

Amino Acids

79.47

Weight (kDa)

5.91

Isoelectric Point (pI)

47.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ARM_ARMC5	PF24768	136 - 389	4.6e-15	ARMC5
ARM_PUB	PF25598	177 - 366	2.1e-07	Plant U-box protein ARM repeat region
Arm	PF00514	234 - 271	5.1e-07	Armadillo/beta-catenin-like repeat
BTB	PF00651	538 - 639	2.4e-24	BTB/POZ domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0000306)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G19330
fragaria_vesca	FvH4_2g23530 FvH4_2g23530 FvH4_2g23530 FvH4_2g23530 FvH4_2g23530 FvH4_2g23530 FvH4_4g15010 FvH4_4g15020 FvH4_4g15040 FvH4_4g15040 FvH4_4g15040 FvH4_4g15040 FvH4_4g15040 FvH4_4g15040
malus_domestica	MD03G1264200.v1.1 MD03G1264700.v1.1 MD11G1284800.v1.1 MD13G1187400.v1.1 MD13G1187500.v1.1 MD13G1187900.v1.1 MD13G1188000.v1.1 MD16G1188100.v1.1 MD16G1188500.v1.1 MD16G1188600.v1.1
prunus_persica	Prupe.1G154900_v2.0.a1 Prupe.1G155100_v2.0.a1 Prupe.1G155200_v2.0.a1 Prupe.1G155300_v2.0.a1 Prupe.1G155300_v2.0.a1 Prupe.8G240700_v2.0.a1
pyrus_communis	pycom03g21190 pycom11g25240 pycom11g25250 pycom13g16210 pycom13g16230 pycom13g16240 pycom16g15820
rosa_chinensis	RchiOBHm_Chr1g0343661 RchiOBHm_Chr4g0416821 RchiOBHm_Chr4g0416831 RchiOBHm_Chr4g0416841 RchiOBHm_Chr4g0416871 RchiOBHm_Chr4g0416951 RchiOBHm_Chr4g0416961 RchiOBHm_Chr6g0291001
rosa_laevigata	RLG00000007939 RLG00000007940 RLG00000007944 RLG00000007945 RLG00000007946 RLG00000007954 RLG00000012156 RLG00000028955
rosa_multiflora	Rmu_co8241865.1_g000001 Rmu_sc0000021.1_g000004 Rmu_sc0000663.1_g000003 Rmu_sc0000663.1_g000009 Rmu_sc0000663.1_g000012 Rmu_sc0000663.1_g000013 Rmu_sc0000663.1_g000021 Rmu_sc0000663.1_g000029 Rmu_sc0014328.1_g000002 Rmu_sc0014328.1_g000003 Rmu_sc0020688.1_g000001 Rmu_sc0024683.1_g000001 Rmu_sc0024683.1_g000002 Rmu_sc0033477.1_g000001 Rmu_sc0035872.1_g000001 Rmu_ssc0000010.1_g000006 Rmu_ssc0000010.1_g000008 Rmu_ssc0000010.1_g000011
rosa_roxburghii	Rroxscaffold_4G00310390 Rroxscaffold_5G00360700 Rroxscaffold_5G00360710 Rroxscaffold_5G00360720 Rroxscaffold_5G00360750 Rroxscaffold_5G00360800 Rroxscaffold_5G00360820 Rroxscaffold_7G00176470
rosa_rugosa	Rorug04G0145200 Rorug04G0145300 Rorug04G0145400 Rorug04G0145400 Rorug04G0145600.1 Rorug04G0145700.1 Rorug04G0148500 Rorug06G0217000
rosa_samantha	Rh2BG232000 Rh4AG207900 Rh4BG205000 Rh4BG205100 Rh4CG218700 Rh4DG134800 Rh4DG204800 Rh4DG204900 Rh4DG205000 Rh4DG205100 Rh4DG205200 Rh4DG205300 Rh4DG205400 Rh4DG205500 Rh5BG434500 Rh5CG457700 Rh6AG326800 Rh6BG334300 Rh6CG340500 Rh6DG327800
rosa_wichuraiana	Rw1G015270 Rw4G017570 Rw4G017580 Rw4G017590 Rw4G017600 Rw4G017610 Rw4G017620 Rw4G017630 Rw4G017640 Rw6G028420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	653
AasI	GACNNNNNNGTC	1 cut(s)	104
Acc36I	ACCTGC	2 cut(s)	157, 653
AccB1I	GGYRCC	3 cut(s)	325, 508, 665
AccII	CGCG	3 cut(s)	19, 162, 2082
AclWI	GGATC	6 cut(s)	349, 761, 774, 1822, 1854, 2067
AcoI	YGGCCR	1 cut(s)	340
AcsI	RAATTY	3 cut(s)	188, 1253, 1677
AcuI	CTGAAG	2 cut(s)	52, 2087
AcyI	GRCGYC	1 cut(s)	326
AfaI	GTAC	5 cut(s)	648, 747, 843, 895, 1523
AfiI	CCNNNNNNNGG	1 cut(s)	872
AflII	CTTAAG	1 cut(s)	1007
AhdI	GACNNNNNGTC	1 cut(s)	407
AjnI	CCWGG	2 cut(s)	396, 741
Alw21I	GWGCWC	1 cut(s)	270
AlwI	GGATC	6 cut(s)	349, 761, 774, 1822, 1854, 2067
AlwNI	CAGNNNCTG	1 cut(s)	178
AoxI	GGCC	7 cut(s)	228, 238, 340, 1368, 1515, 1882, 1962
ApeKI	GCWGC	8 cut(s)	659, 914, 938, 1163, 1565, 1859, 2128, 2131
ApoI	RAATTY	3 cut(s)	188, 1253, 1677
ArsI	GACNNNNNNTTYG	2 cut(s)	357, 389
AseI	ATTAAT	1 cut(s)	995
AspLEI	GCGC	2 cut(s)	328, 2084
AspS9I	GGNCC	2 cut(s)	97, 1882
AsuHPI	GGTGA	7 cut(s)	101, 128, 241, 495, 1005, 1569, 1613
AsuII	TTCGAA	1 cut(s)	120
AvaII	GGWCC	1 cut(s)	97
BaeGI	GKGCMC	1 cut(s)	670
BaeI	ACNNNNGTAYC	2 cut(s)	2027, 2060
BamHI	GGATCC	1 cut(s)	766
BanI	GGYRCC	3 cut(s)	325, 508, 665
BanII	GRGCYC	1 cut(s)	270
BarI	GAAGNNNNNNTAC	2 cut(s)	738, 770
BauI	CACGAG	4 cut(s)	269, 368, 434, 1554
BbsI	GAAGAC	2 cut(s)	69, 109
Bbv12I	GWGCWC	1 cut(s)	270
BbvI	GCAGC	8 cut(s)	646, 901, 925, 1150, 1577, 1846, 2140, 2143
BccI	CCATC	5 cut(s)	1241, 1344, 1582, 1715, 1767
BceAI	ACGGC	3 cut(s)	8, 355, 1907
BciT130I	CCWGG	2 cut(s)	398, 743
BciVI	GTATCC	1 cut(s)	2055
BclI	TGATCA	2 cut(s)	522, 1864
BfaI	CTAG	3 cut(s)	1272, 1304, 1416
BfmI	CTRYAG	1 cut(s)	660
BfoI	RGCGCY	1 cut(s)	329
BfrI	CTTAAG	1 cut(s)	1007
BfuAI	ACCTGC	2 cut(s)	157, 653
BfuI	GTATCC	1 cut(s)	2055
BglI	GCCNNNNNGGC	1 cut(s)	227
BglII	AGATCT	1 cut(s)	142
BmcAI	AGTACT	1 cut(s)	843
Bme1390I	CCNGG	2 cut(s)	398, 743
Bme18I	GGWCC	1 cut(s)	97
BmeRI	GACNNNNNGTC	1 cut(s)	407
BmgT120I	GGNCC	2 cut(s)	97, 1882
BmiI	GGNNCC	4 cut(s)	327, 510, 667, 768
BmrFI	CCNGG	2 cut(s)	398, 743
BpiI	GAAGAC	2 cut(s)	69, 109
BplI	GAGNNNNNCTC	2 cut(s)	2108, 2140
BpmI	CTGGAG	3 cut(s)	200, 440, 2030
Bpu10I	CCTNAGC	1 cut(s)	1842
Bpu14I	TTCGAA	1 cut(s)	120
BsaAI	YACGTR	1 cut(s)	1612
BsaBI	GATNNNNATC	1 cut(s)	1869
BsaHI	GRCGYC	1 cut(s)	326
BsaJI	CCNNGG	7 cut(s)	235, 255, 396, 397, 741, 1484, 2039
BsaWI	WCCGGW	2 cut(s)	94, 99
BsaXI	ACNNNNNCTCC	2 cut(s)	520, 550
Bsc4I	CCNNNNNNNGG	1 cut(s)	872
Bse118I	RCCGGY	2 cut(s)	328, 505
Bse1I	ACTGG	2 cut(s)	1533, 2013
Bse3DI	GCAATG	4 cut(s)	552, 1089, 1257, 2145
Bse8I	GATNNNNATC	1 cut(s)	1869
BseBI	CCWGG	2 cut(s)	398, 743
BseDI	CCNNGG	7 cut(s)	235, 255, 396, 397, 741, 1484, 2039
BseGI	GGATG	3 cut(s)	818, 1567, 1747
BseJI	GATNNNNATC	1 cut(s)	1869
BseLI	CCNNNNNNNGG	1 cut(s)	872
BseMI	GCAATG	4 cut(s)	552, 1089, 1257, 2145
BseMII	CTCAG	5 cut(s)	753, 895, 1109, 1856, 1867
BseNI	ACTGG	2 cut(s)	1533, 2013
BseRI	GAGGAG	5 cut(s)	278, 600, 1322, 1560, 1841
BseSI	GKGCMC	1 cut(s)	670
BseX3I	CGGCCG	1 cut(s)	340
BseXI	GCAGC	8 cut(s)	646, 901, 925, 1150, 1577, 1846, 2140, 2143
BsgI	GTGCAG	1 cut(s)	2082
Bsh1236I	CGCG	3 cut(s)	19, 162, 2082
Bsh1285I	CGRYCG	2 cut(s)	211, 343
BshFI	GGCC	7 cut(s)	230, 240, 342, 1370, 1517, 1884, 1964
BshNI	GGYRCC	3 cut(s)	325, 508, 665
BsiEI	CGRYCG	2 cut(s)	211, 343
BsiHKAI	GWGCWC	1 cut(s)	270
BsiSI	CCGG	6 cut(s)	95, 100, 302, 329, 447, 506
BslFI	GGGAC	3 cut(s)	92, 414, 1763
BslI	CCNNNNNNNGG	1 cut(s)	872
BsmFI	GGGAC	3 cut(s)	92, 414, 1763
BsmI	GAATGC	3 cut(s)	1283, 1703, 1995
BsnI	GGCC	7 cut(s)	230, 240, 342, 1370, 1517, 1884, 1964
Bsp119I	TTCGAA	1 cut(s)	120
Bsp1286I	GDGCHC	2 cut(s)	270, 670
Bsp1407I	TGTACA	1 cut(s)	1521
Bsp19I	CCATGG	2 cut(s)	235, 2039
Bsp68I	TCGCGA	1 cut(s)	162
BspANI	GGCC	7 cut(s)	230, 240, 342, 1370, 1517, 1884, 1964
BspCNI	CTCAG	5 cut(s)	754, 894, 1108, 1855, 1866
BspFNI	CGCG	3 cut(s)	19, 162, 2082
BspLI	GGNNCC	4 cut(s)	327, 510, 667, 768
BspMAI	CTGCAG	1 cut(s)	664
BspMI	ACCTGC	2 cut(s)	157, 653
BspPI	GGATC	6 cut(s)	349, 761, 774, 1822, 1854, 2067
BspT104I	TTCGAA	1 cut(s)	120
BspT107I	GGYRCC	3 cut(s)	325, 508, 665
BspTI	CTTAAG	1 cut(s)	1007
BsrDI	GCAATG	4 cut(s)	552, 1089, 1257, 2145
BsrFI	RCCGGY	2 cut(s)	328, 505
BsrGI	TGTACA	1 cut(s)	1521
BsrI	ACTGG	2 cut(s)	1533, 2013
BssAI	RCCGGY	2 cut(s)	328, 505
BssECI	CCNNGG	7 cut(s)	235, 255, 396, 397, 741, 1484, 2039
BssNI	GRCGYC	1 cut(s)	326
BssSI	CACGAG	4 cut(s)	269, 368, 434, 1554
BssT1I	CCWWGG	3 cut(s)	235, 255, 2039
Bst2BI	CACGAG	4 cut(s)	269, 368, 434, 1554
Bst2UI	CCWGG	2 cut(s)	398, 743
Bst4CI	ACNGT	7 cut(s)	290, 350, 386, 893, 1488, 1508, 1662
Bst6I	CTCTTC	2 cut(s)	255, 1375
BstACI	GRCGYC	1 cut(s)	326
BstAFI	CTTAAG	1 cut(s)	1007
BstAPI	GCANNNNNTGC	2 cut(s)	515, 1709
BstAUI	TGTACA	1 cut(s)	1521
BstBAI	YACGTR	1 cut(s)	1612
BstBI	TTCGAA	1 cut(s)	120
BstDEI	CTNAG	8 cut(s)	576, 762, 881, 1095, 1465, 1645, 1842, 1853
BstDSI	CCRYGG	3 cut(s)	235, 1484, 2039
BstF5I	GGATG	3 cut(s)	818, 1567, 1747
BstFNI	CGCG	3 cut(s)	19, 162, 2082
BstH2I	RGCGCY	1 cut(s)	329
BstHHI	GCGC	2 cut(s)	328, 2084
BstMCI	CGRYCG	2 cut(s)	211, 343
BstNI	CCWGG	2 cut(s)	398, 743
BstNSI	RCATGY	2 cut(s)	1907, 1995
BstSCI	CCNGG	2 cut(s)	396, 741
BstSFI	CTRYAG	1 cut(s)	660
BstSLI	GKGCMC	1 cut(s)	670
BstSNI	TACGTA	1 cut(s)	1612
BstUI	CGCG	3 cut(s)	19, 162, 2082
BstV1I	GCAGC	8 cut(s)	646, 901, 925, 1150, 1577, 1846, 2140, 2143
BstV2I	GAAGAC	2 cut(s)	69, 109
BstX2I	RGATCY	3 cut(s)	142, 766, 1846
BstXI	CCANNNNNNTGG	1 cut(s)	566
BstYI	RGATCY	3 cut(s)	142, 766, 1846
BstZI	CGGCCG	1 cut(s)	340
BsuI	GTATCC	1 cut(s)	2055
BsuRI	GGCC	7 cut(s)	230, 240, 342, 1370, 1517, 1884, 1964
BtgI	CCRYGG	3 cut(s)	235, 1484, 2039
BtsCI	GGATG	3 cut(s)	818, 1567, 1747
BtsI	GCAGTG	1 cut(s)	1203
BtsIMutI	CAGTG	1 cut(s)	1203
BtuMI	TCGCGA	1 cut(s)	162
BveI	ACCTGC	2 cut(s)	157, 653
CaiI	CAGNNNCTG	1 cut(s)	178
CfoI	GCGC	2 cut(s)	328, 2084
Cfr10I	RCCGGY	2 cut(s)	328, 505
Cfr13I	GGNCC	2 cut(s)	97, 1882
CpoI	CGGWCCG	1 cut(s)	97
CseI	GACGC	1 cut(s)	25
Csp6I	GTAC	5 cut(s)	647, 746, 842, 894, 1522
CspCI	CAANNNNNGTGG	2 cut(s)	1661, 1696
CspI	CGGWCCG	1 cut(s)	97
CviAII	CATG	9 cut(s)	236, 560, 1502, 1714, 1904, 1945, 1975, 1992, 2040
CviQI	GTAC	5 cut(s)	647, 746, 842, 894, 1522
DdeI	CTNAG	8 cut(s)	576, 762, 881, 1095, 1465, 1645, 1842, 1853
DinI	GGCGCC	1 cut(s)	327
DraI	TTTAAA	3 cut(s)	688, 987, 1332
DrdI	GACNNNNNNGTC	1 cut(s)	104
DriI	GACNNNNNGTC	1 cut(s)	407
DseDI	GACNNNNNNGTC	1 cut(s)	104
EaeI	YGGCCR	1 cut(s)	340
EagI	CGGCCG	1 cut(s)	340
Eam1104I	CTCTTC	2 cut(s)	255, 1375
Eam1105I	GACNNNNNGTC	1 cut(s)	407
EarI	CTCTTC	2 cut(s)	255, 1375
Ecl136II	GAGCTC	1 cut(s)	268
EclXI	CGGCCG	1 cut(s)	340
Eco105I	TACGTA	1 cut(s)	1612
Eco130I	CCWWGG	3 cut(s)	235, 255, 2039
Eco147I	AGGCCT	1 cut(s)	1964
Eco24I	GRGCYC	1 cut(s)	270
Eco32I	GATATC	1 cut(s)	2070
Eco47I	GGWCC	1 cut(s)	97
Eco52I	CGGCCG	1 cut(s)	340
Eco53kI	GAGCTC	1 cut(s)	268
Eco57I	CTGAAG	2 cut(s)	52, 2087
EcoICRI	GAGCTC	1 cut(s)	268
EcoO109I	RGGNCCY	1 cut(s)	1882
EcoRI	GAATTC	1 cut(s)	1253
EcoRII	CCWGG	2 cut(s)	396, 741
EcoRV	GATATC	1 cut(s)	2070
EcoT14I	CCWWGG	3 cut(s)	235, 255, 2039
EcoT22I	ATGCAT	2 cut(s)	1705, 1997
EcoT38I	GRGCYC	1 cut(s)	270
EgeI	GGCGCC	1 cut(s)	327
EheI	GGCGCC	1 cut(s)	327
ErhI	CCWWGG	3 cut(s)	235, 255, 2039
FaeI	CATG	9 cut(s)	239, 563, 1505, 1717, 1907, 1948, 1978, 1995, 2043
FalI	AAGNNNNNCTT	2 cut(s)	1372, 1404
FaqI	GGGAC	3 cut(s)	92, 414, 1763
FatI	CATG	9 cut(s)	235, 559, 1501, 1713, 1903, 1944, 1974, 1991, 2039
FauI	CCCGC	5 cut(s)	117, 315, 483, 1347, 2056
FbaI	TGATCA	2 cut(s)	522, 1864
FokI	GGATG	3 cut(s)	805, 1574, 1754
FriOI	GRGCYC	1 cut(s)	270
FspBI	CTAG	3 cut(s)	1272, 1304, 1416
GlaI	GCGC	2 cut(s)	327, 2083
GsuI	CTGGAG	3 cut(s)	200, 440, 2030
HaeII	RGCGCY	1 cut(s)	329
HaeIII	GGCC	7 cut(s)	230, 240, 342, 1370, 1517, 1884, 1964
HapII	CCGG	6 cut(s)	95, 100, 302, 329, 447, 506
HgaI	GACGC	1 cut(s)	25
HhaI	GCGC	2 cut(s)	328, 2084
Hin1I	GRCGYC	1 cut(s)	326
Hin1II	CATG	9 cut(s)	239, 563, 1505, 1717, 1907, 1948, 1978, 1995, 2043
Hin6I	GCGC	2 cut(s)	326, 2082
HinP1I	GCGC	2 cut(s)	326, 2082
HincII	GTYRAC	1 cut(s)	2032
HindII	GTYRAC	1 cut(s)	2032
HinfI	GANTC	7 cut(s)	175, 182, 500, 603, 676, 1759, 1800
HpaII	CCGG	6 cut(s)	95, 100, 302, 329, 447, 506
HphI	GGTGA	7 cut(s)	101, 128, 241, 495, 1005, 1569, 1613
Hpy166II	GTNNAC	8 cut(s)	92, 274, 286, 814, 1322, 1615, 1658, 2032
Hpy188III	TCNNGA	9 cut(s)	161, 179, 376, 526, 589, 832, 1244, 1844, 1916
Hpy8I	GTNNAC	8 cut(s)	92, 274, 286, 814, 1322, 1615, 1658, 2032
Hpy99I	CGWCG	1 cut(s)	152
HpyAV	CCTTC	3 cut(s)	590, 680, 1646
HpyCH4III	ACNGT	7 cut(s)	290, 350, 386, 893, 1488, 1508, 1662
HpyCH4IV	ACGT	2 cut(s)	1611, 1752
HpyF3I	CTNAG	8 cut(s)	576, 762, 881, 1095, 1465, 1645, 1842, 1853
HpySE526I	ACGT	2 cut(s)	1611, 1752
Hsp92I	GRCGYC	1 cut(s)	326
Hsp92II	CATG	9 cut(s)	239, 563, 1505, 1717, 1907, 1948, 1978, 1995, 2043
HspAI	GCGC	2 cut(s)	326, 2082
KasI	GGCGCC	1 cut(s)	325
KroI	GCCGGC	1 cut(s)	328
KroNI	GCCGGC	1 cut(s)	330
Ksp22I	TGATCA	2 cut(s)	522, 1864
LmnI	GCTCC	5 cut(s)	265, 293, 449, 1573, 2011
Lsp1109I	GCAGC	8 cut(s)	646, 901, 925, 1150, 1577, 1846, 2140, 2143
MaeI	CTAG	3 cut(s)	1272, 1304, 1416
MaeII	ACGT	2 cut(s)	1611, 1752
MaeIII	GTNAC	5 cut(s)	25, 1011, 1418, 1727, 1980
MboII	GAAGA	9 cut(s)	71, 74, 86, 109, 272, 625, 1324, 1373, 1392
MfeI	CAATTG	1 cut(s)	543
MflI	RGATCY	3 cut(s)	142, 766, 1846
MhlI	GDGCHC	2 cut(s)	270, 670
Mly113I	GGCGCC	1 cut(s)	326
MlyI	GAGTC	2 cut(s)	191, 670
MmeI	TCCRAC	3 cut(s)	231, 912, 1177
Mph1103I	ATGCAT	2 cut(s)	1705, 1997
MreI	CGCCGGCG	1 cut(s)	328
MroNI	GCCGGC	1 cut(s)	328
MslI	CAYNNNNRTG	3 cut(s)	281, 564, 1925
MspCI	CTTAAG	1 cut(s)	1007
MspI	CCGG	6 cut(s)	95, 100, 302, 329, 447, 506
MspR9I	CCNGG	2 cut(s)	398, 743
MunI	CAATTG	1 cut(s)	543
Mva1269I	GAATGC	3 cut(s)	1283, 1703, 1995
MvaI	CCWGG	2 cut(s)	398, 743
MvnI	CGCG	3 cut(s)	19, 162, 2082
NaeI	GCCGGC	1 cut(s)	330
NarI	GGCGCC	1 cut(s)	326
NcoI	CCATGG	2 cut(s)	235, 2039
NgoMIV	GCCGGC	1 cut(s)	328
NlaIII	CATG	9 cut(s)	239, 563, 1505, 1717, 1907, 1948, 1978, 1995, 2043
NlaIV	GGNNCC	4 cut(s)	327, 510, 667, 768
NmuCI	GTSAC	3 cut(s)	25, 1011, 1418
NruI	TCGCGA	1 cut(s)	162
NsiI	ATGCAT	2 cut(s)	1705, 1997
NspI	RCATGY	2 cut(s)	1907, 1995
NspV	TTCGAA	1 cut(s)	120
PaeI	GCATGC	2 cut(s)	1907, 1995
PaqCI	CACCTGC	1 cut(s)	653
PasI	CCCWGGG	1 cut(s)	397
PceI	AGGCCT	1 cut(s)	1964
PctI	GAATGC	3 cut(s)	1283, 1703, 1995
PdiI	GCCGGC	1 cut(s)	330
PfeI	GAWTC	5 cut(s)	175, 500, 603, 1759, 1800
PleI	GAGTC	2 cut(s)	190, 670
PluTI	GGCGCC	1 cut(s)	329
PpsI	GAGTC	2 cut(s)	190, 670
Ppu21I	YACGTR	1 cut(s)	1612
PshBI	ATTAAT	1 cut(s)	995
Psp124BI	GAGCTC	1 cut(s)	270
Psp6I	CCWGG	2 cut(s)	396, 741
PspGI	CCWGG	2 cut(s)	396, 741
PspN4I	GGNNCC	4 cut(s)	327, 510, 667, 768
PspPI	GGNCC	2 cut(s)	97, 1882
PstI	CTGCAG	1 cut(s)	664
PstNI	CAGNNNCTG	1 cut(s)	178
PsuI	RGATCY	3 cut(s)	142, 766, 1846
RruI	TCGCGA	1 cut(s)	162
RsaI	GTAC	5 cut(s)	648, 747, 843, 895, 1523
RsaNI	GTAC	5 cut(s)	647, 746, 842, 894, 1522
RseI	CAYNNNNRTG	3 cut(s)	281, 564, 1925
Rsr2I	CGGWCCG	1 cut(s)	97
RsrII	CGGWCCG	1 cut(s)	97
SacI	GAGCTC	1 cut(s)	270
Sau96I	GGNCC	2 cut(s)	97, 1882
ScaI	AGTACT	1 cut(s)	843
SchI	GAGTC	2 cut(s)	191, 670
ScrFI	CCNGG	2 cut(s)	398, 743
SduI	GDGCHC	2 cut(s)	270, 670
SfcI	CTRYAG	1 cut(s)	660
SfoI	GGCGCC	1 cut(s)	327
SfuI	TTCGAA	1 cut(s)	120
SgrAI	CRCCGGYG	1 cut(s)	328
SinI	GGWCC	1 cut(s)	97
SmiMI	CAYNNNNRTG	3 cut(s)	281, 564, 1925
SmlI	CTYRAG	1 cut(s)	1007
SmoI	CTYRAG	1 cut(s)	1007
SnaBI	TACGTA	1 cut(s)	1612
SphI	GCATGC	2 cut(s)	1907, 1995
SseBI	AGGCCT	1 cut(s)	1964
SspDI	GGCGCC	1 cut(s)	325
SspI	AATATT	1 cut(s)	1768
SspMI	CTAG	3 cut(s)	1272, 1304, 1416
SstI	GAGCTC	1 cut(s)	270
StuI	AGGCCT	1 cut(s)	1964
StyD4I	CCNGG	2 cut(s)	396, 741
StyI	CCWWGG	3 cut(s)	235, 255, 2039
TaaI	ACNGT	7 cut(s)	290, 350, 386, 893, 1488, 1508, 1662
TaiI	ACGT	2 cut(s)	1614, 1755
TaqI	TCGA	4 cut(s)	57, 120, 375, 1122
TatI	WGTACW	2 cut(s)	841, 1521
TauI	GCSGC	3 cut(s)	233, 335, 659
TfiI	GAWTC	5 cut(s)	175, 500, 603, 1759, 1800
TscAI	CASTG	1 cut(s)	1210
TseFI	GTSAC	3 cut(s)	25, 1011, 1418
TseI	GCWGC	8 cut(s)	659, 914, 938, 1163, 1565, 1859, 2128, 2131
Tsp45I	GTSAC	3 cut(s)	25, 1011, 1418
TspDTI	ATGAA	4 cut(s)	576, 711, 1246, 1792
TspGWI	ACGGA	2 cut(s)	911, 1697
TspRI	CASTG	1 cut(s)	1210
Vha464I	CTTAAG	1 cut(s)	1007
VpaK11BI	GGWCC	1 cut(s)	97
VspI	ATTAAT	1 cut(s)	995
XapI	RAATTY	3 cut(s)	188, 1253, 1677
XceI	RCATGY	2 cut(s)	1907, 1995
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1770
XspI	CTAG	3 cut(s)	1272, 1304, 1416
ZrmI	AGTACT	1 cut(s)	843
Zsp2I	ATGCAT	2 cut(s)	1705, 1997

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.