Rw4G017580

Carboxylesterase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000306
Physical Location & Seq
Forward (+)
39538721 .. 39540674
1954 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G017580.1

Sequence Viewer

Length: 1905 bp
ATGGGTTCCCTTGCCAAGGAGATAGACAGAGAGGTCATCCCATTTGTCCGTGTTTTCAAGGACGGCTCAGTAGAAAGACTACTGGGTTCTCCTTATAAAGCACCATTGCTTGATGATCCAGAATCTGGAGTCTCATCCAAAGATGTCACCATTTCGAAAAACCCTTTGATCTCAGCCAGAATCTTCCTCCCTAAACTCGACGAAGAGAAGCCACACAAGAAGCTCCCCGTCTTGGTTTACTGCCATGGTGGAGGTTTCTGCATCGAGTCCGCCTTCTCTTTTGATCACCACCGGTTTCTCGACGGCTTGGTGTCCCAAGCTCAAGTTGTTGCTGTTTCTGTGGAGTACAGGATGGCTCCTGAGCACCCTCTTCCTGCCTGCTATGAAGATTGTTGGGCTGCCCTGCAATGGGTTGCTTCACACTTCATTGAAAATGGTATCAGTGAGGAGCCTTGGTTGACGAATCACGGCAACCCAGAACGTTTTTTCCTTGGTGGTGATAGTGCAGGAGCTAACATTGCTCATAATCTGGCAATGAGAGTTGGCAAAGAGGGCTTGCCTGGTGGTTTGAAGATATTGGGAGCCTCTCTTACACACCCCTACTTCTGGGGTTCCAAGACTATTGCGGAAGACCCTTGTGAAGAACCTGAGAAGGATTTGGCAGCTATGCTTTGGAACTTTGCGTACCCCTTAGCACCTGGTGGACTTGATAATCCATTGATCAATCCTCTTGCTCCTGAAGCACCAAGCTTGGCAGGACTTGGGTGTTCTAGGTTGTTTGTGAGCGTCTCGGAACATGATGGGCTAAAGTTTAGAGGCATTCGGTACTACGATGCCGTGAAGAAAAGTGGGTGGGAAGGAGAGGCAGAGCTGGTGGACGTGGAAGGAGAGGCTCATGCATTTCATATTTTGAAATTTGAGACTCAGAAAGCCAAGGATTTGACAAAAAAGTTGGTTGATTTTCTCAAGGTAGAAGACAAGAAGGTAGAGAAGGAGCTAGACAAAGAGCTGATCCCATTTGTCCGAGTCTACAAGGATGGCTCAGTTGAACGACTCATGGGCACTCCATATGTACCCCCAATGCTTAATGATCCAGTATCAGGAGTAGTCTTCCTCCCAAAACTTGATGCTGATCAAACCCACCAGAAACTCCCCATCTTGGTCTACTACCATGGAGGGGCTTTTATGATCGAATCCGCCTTCTCGTTCGATCACCACAGGTATCTTGACAGCTTGGTATCCCAAGGCAAAGTTGTTGCTGTATCAGTTGAATACAGGATGGCTCCTGAGCACCCTCTTCCTGCAGGCTATGAAGATTGTTGGGCTGCCCTCCAATGGGTTGCCTCACACTTCATTGATAATGGTATCAGTGAAGAGCCCTGGTTGACAAATCATGGTAATCCGGACAGGTTTTTTCTTGGCGGCGATAGTGCAGGTGCCAACATTGCTCATAATGTGGCCATGAGAGTTGGGAAAGAGGGTTTGCCTGGTGGTCTTAAAATACTGGGAACTTCTCTTACACACCCTTATTTTTGGGGTTCCAAGCCAATCGATGAATTAGACCCTTGCAAAGAGCCTGAGAAGGATTTAGGGTGTTTCGTTTGGAACTTCGCGTACCCCTCGGTACCTGGTGGGATTGATAATCCAATGATGAATCCTTTTAGTCCAGAAGCACCAAGCTTGGCTGGACTTGGGTGTTCAAGGTTGTTTGTTAGTGTTTCTGAGAACGATACGCTGAGATATAGAGGTGTTCATTACCATGAGGCAGTGAAGAAAAGTGGTTGGAAAGGTGAAGCAGAGTTGGTTGAAGTGGAAGGAGAGGAACATGCATTTCATATTCTGAAATTTGAGACTCCGAAGGCTCAGGCTTTGACCAAAAAAGTGGCCGAGTTTCTCCTCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

634

Amino Acids

70.08

Weight (kDa)

5.47

Isoelectric Point (pI)

33.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 61 - 186 3.6e-09 Carboxylesterase family
BD-FAE PF20434 70 - 182 1.7e-10 BD-FAE
Abhydrolase_3 PF07859 78 - 302 1.7e-46 alpha/beta hydrolase fold
COesterase PF00135 370 - 499 4.1e-09 Carboxylesterase family
BD-FAE PF20434 379 - 492 3.1e-10 BD-FAE
Abhydrolase_3 PF07859 387 - 612 7e-45 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000306)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19330
fragaria_vesca FvH4_2g23530 FvH4_2g23530 FvH4_2g23530 FvH4_2g23530 FvH4_2g23530 FvH4_2g23530 FvH4_4g15010 FvH4_4g15020 FvH4_4g15040 FvH4_4g15040 FvH4_4g15040 FvH4_4g15040 FvH4_4g15040 FvH4_4g15040
malus_domestica MD03G1264200.v1.1 MD03G1264700.v1.1 MD11G1284800.v1.1 MD13G1187400.v1.1 MD13G1187500.v1.1 MD13G1187900.v1.1 MD13G1188000.v1.1 MD16G1188100.v1.1 MD16G1188500.v1.1 MD16G1188600.v1.1
prunus_persica Prupe.1G154900_v2.0.a1 Prupe.1G155100_v2.0.a1 Prupe.1G155200_v2.0.a1 Prupe.1G155300_v2.0.a1 Prupe.1G155300_v2.0.a1 Prupe.8G240700_v2.0.a1
pyrus_communis pycom03g21190 pycom11g25240 pycom11g25250 pycom13g16210 pycom13g16230 pycom13g16240 pycom16g15820
rosa_chinensis RchiOBHm_Chr1g0343661 RchiOBHm_Chr4g0416821 RchiOBHm_Chr4g0416831 RchiOBHm_Chr4g0416841 RchiOBHm_Chr4g0416871 RchiOBHm_Chr4g0416951 RchiOBHm_Chr4g0416961 RchiOBHm_Chr6g0291001
rosa_laevigata RLG00000007939 RLG00000007940 RLG00000007944 RLG00000007945 RLG00000007946 RLG00000007954 RLG00000012156 RLG00000028955
rosa_multiflora Rmu_co8241865.1_g000001 Rmu_sc0000021.1_g000004 Rmu_sc0000663.1_g000003 Rmu_sc0000663.1_g000009 Rmu_sc0000663.1_g000012 Rmu_sc0000663.1_g000013 Rmu_sc0000663.1_g000021 Rmu_sc0000663.1_g000029 Rmu_sc0014328.1_g000002 Rmu_sc0014328.1_g000003 Rmu_sc0020688.1_g000001 Rmu_sc0024683.1_g000001 Rmu_sc0024683.1_g000002 Rmu_sc0033477.1_g000001 Rmu_sc0035872.1_g000001 Rmu_ssc0000010.1_g000006 Rmu_ssc0000010.1_g000008 Rmu_ssc0000010.1_g000011
rosa_roxburghii Rroxscaffold_4G00310390 Rroxscaffold_5G00360700 Rroxscaffold_5G00360710 Rroxscaffold_5G00360720 Rroxscaffold_5G00360750 Rroxscaffold_5G00360800 Rroxscaffold_5G00360820 Rroxscaffold_7G00176470
rosa_rugosa Rorug04G0145200 Rorug04G0145300 Rorug04G0145400 Rorug04G0145400 Rorug04G0145600.1 Rorug04G0145700.1 Rorug04G0148500 Rorug06G0217000
rosa_samantha Rh2BG232000 Rh4AG207900 Rh4BG205000 Rh4BG205100 Rh4CG218700 Rh4DG134800 Rh4DG204800 Rh4DG204900 Rh4DG205000 Rh4DG205100 Rh4DG205200 Rh4DG205300 Rh4DG205400 Rh4DG205500 Rh5BG434500 Rh5CG457700 Rh6AG326800 Rh6BG334300 Rh6CG340500 Rh6DG327800
rosa_wichuraiana Rw1G015270 Rw4G017570 Rw4G017580 Rw4G017590 Rw4G017600 Rw4G017610 Rw4G017620 Rw4G017630 Rw4G017640 Rw6G028420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 96
AarI CACCTGC 1 cut(s) 1424
AasI GACNNNNNNGTC 1 cut(s) 32
Acc36I ACCTGC 1 cut(s) 1424
Acc65I GGTACC 1 cut(s) 1624
AccB1I GGYRCC 2 cut(s) 1436, 1624
AccB7I CCANNNNNTGG 1 cut(s) 125
AccI GTMKAC 2 cut(s) 1029, 1164
AccII CGCG 1 cut(s) 1613
AccIII TCCGGA 1 cut(s) 1402
AciI CCGC 4 cut(s) 270, 626, 1197, 1422
AclI AACGTT 1 cut(s) 481
AclWI GGATC 3 cut(s) 110, 1006, 1085
AcoI YGGCCR 2 cut(s) 1458, 1884
AcsI RAATTY 2 cut(s) 914, 1844
AcuI CTGAAG 1 cut(s) 759
AdeI CACNNNGTG 1 cut(s) 701
AfaI GTAC 6 cut(s) 347, 686, 827, 1074, 1616, 1626
AgeI ACCGGT 1 cut(s) 291
AgsI TTSAA 8 cut(s) 58, 431, 571, 913, 1049, 1271, 1701, 1808
AjiI CACGTC 1 cut(s) 880
AjnI CCWGG 5 cut(s) 559, 697, 1379, 1486, 1627
AloI GAACNNNNNNTCC 2 cut(s) 471, 503
Alw21I GWGCWC 2 cut(s) 366, 1293
Alw26I GTCTC 4 cut(s) 136, 793, 914, 1844
AlwI GGATC 3 cut(s) 110, 1006, 1085
AlwNI CAGNNNCTG 1 cut(s) 125
Aor13HI TCCGGA 1 cut(s) 1402
AoxI GGCC 2 cut(s) 1458, 1884
ApeKI GCWGC 3 cut(s) 398, 662, 1325
ApoI RAATTY 2 cut(s) 914, 1844
AsiGI ACCGGT 1 cut(s) 291
Asp718I GGTACC 1 cut(s) 1624
AsuHPI GGTGA 5 cut(s) 139, 278, 509, 1205, 1802
AsuII TTCGAA 1 cut(s) 155
BaeGI GKGCMC 1 cut(s) 1064
BalI TGGCCA 1 cut(s) 1460
BanI GGYRCC 2 cut(s) 1436, 1624
BanII GRGCYC 1 cut(s) 1380
BbsI GAAGAC 3 cut(s) 636, 981, 1102
Bbv12I GWGCWC 2 cut(s) 366, 1293
BbvI GCAGC 3 cut(s) 385, 674, 1312
BccI CCATC 5 cut(s) 346, 794, 1031, 1163, 1273
BceAI ACGGC 4 cut(s) 79, 319, 484, 821
BciT130I CCWGG 5 cut(s) 561, 699, 1381, 1488, 1629
BciVI GTATCC 1 cut(s) 1249
BclI TGATCA 3 cut(s) 283, 720, 1132
BcoDI GTCTC 4 cut(s) 136, 793, 914, 1844
BfaI CTAG 2 cut(s) 771, 998
BfmI CTRYAG 1 cut(s) 1302
BfuAI ACCTGC 1 cut(s) 1424
BfuI GTATCC 1 cut(s) 1249
BisI GCNGC 4 cut(s) 399, 663, 1326, 1423
BlsI GCNGC 4 cut(s) 400, 664, 1327, 1424
Bme1390I CCNGG 5 cut(s) 561, 699, 1381, 1488, 1629
BmgBI CACGTC 1 cut(s) 880
BmiI GGNNCC 9 cut(s) 7, 357, 450, 583, 613, 1284, 1438, 1540, 1626
BmrFI CCNGG 5 cut(s) 561, 699, 1381, 1488, 1629
BmrI ACTGGG 2 cut(s) 92, 1514
BmsI GCATC 3 cut(s) 270, 823, 1117
BmuI ACTGGG 2 cut(s) 92, 1514
BpiI GAAGAC 3 cut(s) 636, 981, 1102
BpmI CTGGAG 1 cut(s) 147
Bpu10I CCTNAGC 4 cut(s) 360, 691, 1287, 1863
Bpu14I TTCGAA 1 cut(s) 155
BpuEI CTTGAG 3 cut(s) 306, 950, 1883
Bsa29I ATCGAT 1 cut(s) 1551
BsaBI GATNNNNATC 1 cut(s) 1131
BsaJI CCNNGG 9 cut(s) 15, 244, 452, 490, 933, 1171, 1243, 1379, 1620
BsaWI WCCGGW 2 cut(s) 291, 1402
BsaXI ACNNNNNCTCC 2 cut(s) 440, 470
Bse118I RCCGGY 1 cut(s) 291
Bse1I ACTGG 3 cut(s) 87, 1094, 1509
Bse3DI GCAATG 5 cut(s) 104, 413, 516, 540, 1443
Bse8I GATNNNNATC 1 cut(s) 1131
BseAI TCCGGA 1 cut(s) 1402
BseBI CCWGG 5 cut(s) 561, 699, 1381, 1488, 1629
BseCI ATCGAT 1 cut(s) 1551
BseDI CCNNGG 9 cut(s) 15, 244, 452, 490, 933, 1171, 1243, 1379, 1620
BseGI GGATG 5 cut(s) 36, 134, 357, 1042, 1284
BseJI GATNNNNATC 1 cut(s) 1131
BseMI GCAATG 5 cut(s) 104, 413, 516, 540, 1443
BseNI ACTGG 3 cut(s) 87, 1094, 1509
BseRI GAGGAG 2 cut(s) 461, 1886
BseSI GKGCMC 1 cut(s) 1064
BseXI GCAGC 3 cut(s) 385, 674, 1312
BsgI GTGCAG 2 cut(s) 525, 1452
Bsh1236I CGCG 1 cut(s) 1613
BshFI GGCC 2 cut(s) 1460, 1886
BshNI GGYRCC 2 cut(s) 1436, 1624
BshTI ACCGGT 1 cut(s) 291
BshVI ATCGAT 1 cut(s) 1551
BsiHKAI GWGCWC 2 cut(s) 366, 1293
BsiSI CCGG 2 cut(s) 292, 1403
BslFI GGGAC 1 cut(s) 298
BsmAI GTCTC 4 cut(s) 136, 793, 914, 1844
BsmBI CGTCTC 1 cut(s) 793
BsmFI GGGAC 1 cut(s) 298
BsmI GAATGC 1 cut(s) 819
BsnI GGCC 2 cut(s) 1460, 1886
Bsp119I TTCGAA 1 cut(s) 155
Bsp1286I GDGCHC 4 cut(s) 366, 1064, 1293, 1380
Bsp13I TCCGGA 1 cut(s) 1402
Bsp143I GATC 9 cut(s) 115, 168, 283, 720, 1011, 1090, 1132, 1188, 1210
Bsp19I CCATGG 2 cut(s) 244, 1171
BspACI CCGC 4 cut(s) 270, 626, 1197, 1422
BspANI GGCC 2 cut(s) 1460, 1886
BspDI ATCGAT 1 cut(s) 1551
BspEI TCCGGA 1 cut(s) 1402
BspFNI CGCG 1 cut(s) 1613
BspLI GGNNCC 9 cut(s) 7, 357, 450, 583, 613, 1284, 1438, 1540, 1626
BspMAI CTGCAG 1 cut(s) 1306
BspMI ACCTGC 1 cut(s) 1424
BspPI GGATC 3 cut(s) 110, 1006, 1085
BspQI GCTCTTC 1 cut(s) 1368
BspT104I TTCGAA 1 cut(s) 155
BspT107I GGYRCC 2 cut(s) 1436, 1624
BsrDI GCAATG 5 cut(s) 104, 413, 516, 540, 1443
BsrFI RCCGGY 1 cut(s) 291
BsrI ACTGG 3 cut(s) 87, 1094, 1509
BssAI RCCGGY 1 cut(s) 291
BssECI CCNNGG 9 cut(s) 15, 244, 452, 490, 933, 1171, 1243, 1379, 1620
BssMI GATC 9 cut(s) 115, 168, 283, 720, 1011, 1090, 1132, 1188, 1210
BssT1I CCWWGG 7 cut(s) 15, 244, 452, 490, 933, 1171, 1243
Bst2UI CCWGG 5 cut(s) 561, 699, 1381, 1488, 1629
Bst6I CTCTTC 4 cut(s) 198, 375, 1302, 1368
BstBI TTCGAA 1 cut(s) 155
BstC8I GCNNGC 3 cut(s) 379, 557, 1306
BstDSI CCRYGG 2 cut(s) 244, 1171
BstENI CCTNNNNNAGG 1 cut(s) 14
BstF5I GGATG 5 cut(s) 36, 134, 357, 1042, 1284
BstFNI CGCG 1 cut(s) 1613
BstKTI GATC 9 cut(s) 118, 171, 286, 723, 1014, 1093, 1135, 1191, 1213
BstMAI GTCTC 4 cut(s) 136, 793, 914, 1844
BstMBI GATC 9 cut(s) 115, 168, 283, 720, 1011, 1090, 1132, 1188, 1210
BstMWI GCNNNNNNNGC 4 cut(s) 518, 552, 740, 1445
BstNI CCWGG 5 cut(s) 561, 699, 1381, 1488, 1629
BstNSI RCATGY 1 cut(s) 1829
BstSCI CCNGG 5 cut(s) 559, 697, 1379, 1486, 1627
BstSFI CTRYAG 1 cut(s) 1302
BstSLI GKGCMC 1 cut(s) 1064
BstUI CGCG 1 cut(s) 1613
BstV1I GCAGC 3 cut(s) 385, 674, 1312
BstV2I GAAGAC 3 cut(s) 636, 981, 1102
BstXI CCANNNNNNTGG 1 cut(s) 1882
Bsu15I ATCGAT 1 cut(s) 1551
BsuI GTATCC 1 cut(s) 1249
BsuRI GGCC 2 cut(s) 1460, 1886
BsuTUI ATCGAT 1 cut(s) 1551
BtgI CCRYGG 2 cut(s) 244, 1171
BtrI CACGTC 1 cut(s) 880
BtsCI GGATG 5 cut(s) 36, 134, 357, 1042, 1284
BtsI GCAGTG 1 cut(s) 1773
BtsIMutI CAGTG 3 cut(s) 448, 1375, 1773
BveI ACCTGC 1 cut(s) 1424
Cac8I GCNNGC 3 cut(s) 379, 557, 1306
CaiI CAGNNNCTG 1 cut(s) 125
Cfr10I RCCGGY 1 cut(s) 291
ClaI ATCGAT 1 cut(s) 1551
CseI GACGC 1 cut(s) 775
CsiI ACCWGGT 2 cut(s) 697, 1627
Csp6I GTAC 6 cut(s) 346, 685, 826, 1073, 1615, 1625
CspAI ACCGGT 1 cut(s) 291
CviAII CATG 9 cut(s) 245, 797, 896, 1057, 1172, 1394, 1462, 1760, 1826
CviQI GTAC 6 cut(s) 346, 685, 826, 1073, 1615, 1625
DpnI GATC 9 cut(s) 117, 170, 285, 722, 1013, 1092, 1134, 1190, 1212
DpnII GATC 9 cut(s) 115, 168, 283, 720, 1011, 1090, 1132, 1188, 1210
DraIII CACNNNGTG 1 cut(s) 701
DrdI GACNNNNNNGTC 1 cut(s) 32
DseDI GACNNNNNNGTC 1 cut(s) 32
EaeI YGGCCR 2 cut(s) 1458, 1884
Eam1104I CTCTTC 4 cut(s) 198, 375, 1302, 1368
EarI CTCTTC 4 cut(s) 198, 375, 1302, 1368
EciI GGCGGA 2 cut(s) 259, 1186
Eco130I CCWWGG 7 cut(s) 15, 244, 452, 490, 933, 1171, 1243
Eco24I GRGCYC 1 cut(s) 1380
Eco57I CTGAAG 1 cut(s) 759
EcoNI CCTNNNNNAGG 1 cut(s) 14
EcoRII CCWGG 5 cut(s) 559, 697, 1379, 1486, 1627
EcoT14I CCWWGG 7 cut(s) 15, 244, 452, 490, 933, 1171, 1243
EcoT22I ATGCAT 2 cut(s) 901, 1831
EcoT38I GRGCYC 1 cut(s) 1380
ErhI CCWWGG 7 cut(s) 15, 244, 452, 490, 933, 1171, 1243
Esp3I CGTCTC 1 cut(s) 793
FaeI CATG 9 cut(s) 248, 800, 899, 1060, 1175, 1397, 1465, 1763, 1829
FaqI GGGAC 1 cut(s) 298
FatI CATG 9 cut(s) 244, 796, 895, 1056, 1171, 1393, 1461, 1759, 1825
FauNDI CATATG 1 cut(s) 1069
FbaI TGATCA 3 cut(s) 283, 720, 1132
FblI GTMKAC 2 cut(s) 1029, 1164
Fnu4HI GCNGC 4 cut(s) 399, 663, 1326, 1423
FokI GGATG 5 cut(s) 23, 121, 364, 1049, 1291
FriOI GRGCYC 1 cut(s) 1380
Fsp4HI GCNGC 4 cut(s) 399, 663, 1326, 1423
FspBI CTAG 2 cut(s) 771, 998
GluI GCNGC 4 cut(s) 399, 663, 1326, 1423
GsuI CTGGAG 1 cut(s) 147
HaeIII GGCC 2 cut(s) 1460, 1886
HapII CCGG 2 cut(s) 292, 1403
HgaI GACGC 1 cut(s) 775
Hin1II CATG 9 cut(s) 248, 800, 899, 1060, 1175, 1397, 1465, 1763, 1829
HincII GTYRAC 2 cut(s) 459, 1386
HindII GTYRAC 2 cut(s) 459, 1386
HindIII AAGCTT 2 cut(s) 748, 1678
HpaII CCGG 2 cut(s) 292, 1403
HphI GGTGA 5 cut(s) 139, 278, 509, 1205, 1802
Hpy166II GTNNAC 7 cut(s) 238, 459, 704, 877, 1030, 1165, 1386
Hpy188I TCNGA 6 cut(s) 793, 927, 1025, 1723, 1842, 1857
Hpy8I GTNNAC 7 cut(s) 238, 459, 704, 877, 1030, 1165, 1386
Hpy99I CGWCG 2 cut(s) 203, 305
HpyCH4IV ACGT 2 cut(s) 481, 879
HpyCH4V TGCA 8 cut(s) 261, 406, 506, 899, 1304, 1433, 1569, 1829
HpyF10VI GCNNNNNNNGC 4 cut(s) 518, 552, 740, 1445
HpySE526I ACGT 2 cut(s) 481, 879
Hsp92II CATG 9 cut(s) 248, 800, 899, 1060, 1175, 1397, 1465, 1763, 1829
Kpn2I TCCGGA 1 cut(s) 1402
KpnI GGTACC 1 cut(s) 1628
Ksp22I TGATCA 3 cut(s) 283, 720, 1132
Kzo9I GATC 9 cut(s) 115, 168, 283, 720, 1011, 1090, 1132, 1188, 1210
LguI GCTCTTC 1 cut(s) 1368
LmnI GCTCC 8 cut(s) 228, 361, 448, 509, 581, 739, 994, 1288
Lsp1109I GCAGC 3 cut(s) 385, 674, 1312
LweI GCATC 3 cut(s) 270, 823, 1117
MabI ACCWGGT 2 cut(s) 697, 1627
MaeI CTAG 2 cut(s) 771, 998
MaeII ACGT 2 cut(s) 481, 879
MaeIII GTNAC 1 cut(s) 145
MalI GATC 9 cut(s) 117, 170, 285, 722, 1013, 1092, 1134, 1190, 1212
MboI GATC 9 cut(s) 115, 168, 283, 720, 1011, 1090, 1132, 1188, 1210
MhlI GDGCHC 4 cut(s) 366, 1064, 1293, 1380
MlsI TGGCCA 1 cut(s) 1460
MluCI AATT 3 cut(s) 914, 1556, 1844
MluNI TGGCCA 1 cut(s) 1460
MlyI GAGTC 6 cut(s) 138, 275, 916, 1035, 1047, 1846
MmeI TCCRAC 1 cut(s) 1763
Mox20I TGGCCA 1 cut(s) 1460
Mph1103I ATGCAT 2 cut(s) 901, 1831
MroI TCCGGA 1 cut(s) 1402
MscI TGGCCA 1 cut(s) 1460
MseI TTAA 2 cut(s) 1086, 1497
MslI CAYNNNNRTG 1 cut(s) 1758
Msp20I TGGCCA 1 cut(s) 1460
MspI CCGG 2 cut(s) 292, 1403
MspR9I CCNGG 5 cut(s) 561, 699, 1381, 1488, 1629
Mva1269I GAATGC 1 cut(s) 819
MvaI CCWGG 5 cut(s) 561, 699, 1381, 1488, 1629
MvnI CGCG 1 cut(s) 1613
MwoI GCNNNNNNNGC 4 cut(s) 518, 552, 740, 1445
NcoI CCATGG 2 cut(s) 244, 1171
NdeI CATATG 1 cut(s) 1069
NdeII GATC 9 cut(s) 115, 168, 283, 720, 1011, 1090, 1132, 1188, 1210
NlaIII CATG 9 cut(s) 248, 800, 899, 1060, 1175, 1397, 1465, 1763, 1829
NlaIV GGNNCC 9 cut(s) 7, 357, 450, 583, 613, 1284, 1438, 1540, 1626
NmuCI GTSAC 1 cut(s) 145
NsiI ATGCAT 2 cut(s) 901, 1831
NspI RCATGY 1 cut(s) 1829
NspV TTCGAA 1 cut(s) 155
PaqCI CACCTGC 1 cut(s) 1424
PciSI GCTCTTC 1 cut(s) 1368
PctI GAATGC 1 cut(s) 819
PfeI GAWTC 5 cut(s) 122, 180, 463, 1193, 1654
PflMI CCANNNNNTGG 1 cut(s) 125
PinAI ACCGGT 1 cut(s) 291
PkrI GCNGC 4 cut(s) 400, 664, 1327, 1424
PleI GAGTC 6 cut(s) 137, 274, 916, 1034, 1047, 1846
PpsI GAGTC 6 cut(s) 137, 274, 916, 1034, 1047, 1846
PsiI TTATAA 1 cut(s) 96
Psp1406I AACGTT 1 cut(s) 481
Psp6I CCWGG 5 cut(s) 559, 697, 1379, 1486, 1627
PspGI CCWGG 5 cut(s) 559, 697, 1379, 1486, 1627
PspN4I GGNNCC 9 cut(s) 7, 357, 450, 583, 613, 1284, 1438, 1540, 1626
PsrI GAACNNNNNNTAC 6 cut(s) 668, 700, 1501, 1533, 1598, 1630
PstI CTGCAG 1 cut(s) 1306
PstNI CAGNNNCTG 1 cut(s) 125
RsaI GTAC 6 cut(s) 347, 686, 827, 1074, 1616, 1626
RsaNI GTAC 6 cut(s) 346, 685, 826, 1073, 1615, 1625
RseI CAYNNNNRTG 1 cut(s) 1758
SapI GCTCTTC 1 cut(s) 1368
SaqAI TTAA 2 cut(s) 1086, 1497
SatI GCNGC 4 cut(s) 399, 663, 1326, 1423
Sau3AI GATC 9 cut(s) 115, 168, 283, 720, 1011, 1090, 1132, 1188, 1210
SbfI CCTGCAGG 1 cut(s) 1306
SchI GAGTC 6 cut(s) 138, 275, 916, 1035, 1047, 1846
ScrFI CCNGG 5 cut(s) 561, 699, 1381, 1488, 1629
SdaI CCTGCAGG 1 cut(s) 1306
SduI GDGCHC 4 cut(s) 366, 1064, 1293, 1380
SexAI ACCWGGT 2 cut(s) 697, 1627
SfaNI GCATC 3 cut(s) 270, 823, 1117
SfcI CTRYAG 1 cut(s) 1302
SfuI TTCGAA 1 cut(s) 155
SmiMI CAYNNNNRTG 1 cut(s) 1758
SmlI CTYRAG 3 cut(s) 321, 965, 1898
SmoI CTYRAG 3 cut(s) 321, 965, 1898
Sse8387I CCTGCAGG 1 cut(s) 1306
Sse9I AATT 3 cut(s) 914, 1556, 1844
SsiI CCGC 4 cut(s) 270, 626, 1197, 1422
SspMI CTAG 2 cut(s) 771, 998
StyD4I CCNGG 5 cut(s) 559, 697, 1379, 1486, 1627
StyI CCWWGG 7 cut(s) 15, 244, 452, 490, 933, 1171, 1243
TaiI ACGT 2 cut(s) 484, 882
TaqI TCGA 7 cut(s) 155, 198, 264, 300, 1191, 1209, 1551
TasI AATT 3 cut(s) 914, 1556, 1844
TatI WGTACW 1 cut(s) 345
TauI GCSGC 1 cut(s) 1425
TfiI GAWTC 5 cut(s) 122, 180, 463, 1193, 1654
Tru1I TTAA 2 cut(s) 1086, 1497
Tru9I TTAA 2 cut(s) 1086, 1497
TscAI CASTG 3 cut(s) 448, 1375, 1773
TseFI GTSAC 1 cut(s) 145
TseI GCWGC 3 cut(s) 398, 662, 1325
Tsp45I GTSAC 1 cut(s) 145
TspDTI ATGAA 9 cut(s) 399, 415, 893, 1326, 1342, 1569, 1667, 1742, 1823
TspGWI ACGGA 1 cut(s) 38
TspRI CASTG 3 cut(s) 448, 1375, 1773
Van91I CCANNNNNTGG 1 cut(s) 125
XagI CCTNNNNNAGG 1 cut(s) 14
XapI RAATTY 2 cut(s) 914, 1844
XceI RCATGY 1 cut(s) 1829
XmiI GTMKAC 2 cut(s) 1029, 1164
XspI CTAG 2 cut(s) 771, 998
Zsp2I ATGCAT 2 cut(s) 901, 1831
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.