MD16G1188500.v1.1

alpha/beta hydrolase fold

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0000306
Physical Location & Seq
Reverse (-)
16339729 .. 16340694
966 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1188500.v1.1.491

Sequence Viewer

Length: 966 bp
ATGGCTTGCATTGCAAAGGAGGTAGAGACAGAGCTTCTCCCCTTTCTTCGAGTCTACAAGGACGGCTATGTCGAGCGACTCTTAGGCTCTCAATACGTTCCCCCGTTGCTCGATGACCCTGAAACGGGAGTCTCCTCCAAAGACATCACCATTTCACAAGACCCCTTGATCAAAGCCAGAGTCTTTCTTCCAAAACTCGATGAACCACATCAGAAACTCCCCGTCTTGGTCTACTACCATGGTGGGGGTTTCTGCGTTGAGTCCGCCTTCTCATCCGATCACCACCGCTATCTTGACGGGTTGGTCTCCCAAGCCAAGGTTGTTGCTGTGTCGGTCGAGTATAGGATGGCTCCTGAACACTATCTCCCTGCTGCATATGAAGACGGCTGGGCTGCTCTCCAATGGATTGCCTCGCATTTCGCTGAAAACGTCATTGATGACGAGCCCTGGTTGACAAATCATGGAAATTCGGAACGTTTTTTTCTCGGTGGAGATAGTGCGGGAGCCAATATTGCTCATAACATTGCCATGAAAGTTGGGAAGGAGGGCTTGCCTGGTGGTTTAAAGGTGTTGGGAGTCTCTCTCACGCACCCTTACTTTTGCGGTTCAAAGGAAATTGGATCGGAGACTTGTGAAGATCCTGAGGACTTTGGATGTCTGGTTTGGAACTTTGTATACCCTTCAGCGCCAGGCGGTGTTGATTGTCCGATGATCAATCCGGTGGCTCCAGACGCACCGAGCCTGGCGGGACTCGCGTGTTCGCGGTTGTTTGTGAGTGTTTCTGAGAATGATGAACTGAGACATAGAGGCATAGCTTACTATGAAGCAGTGAAGGAAAGTGGGTGGGAAGGAGAAGCTGAGCTGGTTGAAGTGGAAGGAGAGGATCATGCATTTCATATTTTGAAATTTGAGACTCCAAAAGCTAAGGATTTGACCAGAAAAGTGGCCGACTTTCTCACCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

322

Amino Acids

35.31

Weight (kDa)

4.86

Isoelectric Point (pI)

41.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 61 - 173 8.1e-08 Carboxylesterase family
BD-FAE PF20434 69 - 182 1e-09 BD-FAE
Abhydrolase_3 PF07859 76 - 299 3.9e-45 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000306)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19330
fragaria_vesca FvH4_2g23530 FvH4_2g23530 FvH4_2g23530 FvH4_2g23530 FvH4_2g23530 FvH4_2g23530 FvH4_4g15010 FvH4_4g15020 FvH4_4g15040 FvH4_4g15040 FvH4_4g15040 FvH4_4g15040 FvH4_4g15040 FvH4_4g15040
malus_domestica MD03G1264200.v1.1 MD03G1264700.v1.1 MD11G1284800.v1.1 MD13G1187400.v1.1 MD13G1187500.v1.1 MD13G1187900.v1.1 MD13G1188000.v1.1 MD16G1188100.v1.1 MD16G1188500.v1.1 MD16G1188600.v1.1
prunus_persica Prupe.1G154900_v2.0.a1 Prupe.1G155100_v2.0.a1 Prupe.1G155200_v2.0.a1 Prupe.1G155300_v2.0.a1 Prupe.1G155300_v2.0.a1 Prupe.8G240700_v2.0.a1
pyrus_communis pycom03g21190 pycom11g25240 pycom11g25250 pycom13g16210 pycom13g16230 pycom13g16240 pycom16g15820
rosa_chinensis RchiOBHm_Chr1g0343661 RchiOBHm_Chr4g0416821 RchiOBHm_Chr4g0416831 RchiOBHm_Chr4g0416841 RchiOBHm_Chr4g0416871 RchiOBHm_Chr4g0416951 RchiOBHm_Chr4g0416961 RchiOBHm_Chr6g0291001
rosa_laevigata RLG00000007939 RLG00000007940 RLG00000007944 RLG00000007945 RLG00000007946 RLG00000007954 RLG00000012156 RLG00000028955
rosa_multiflora Rmu_co8241865.1_g000001 Rmu_sc0000021.1_g000004 Rmu_sc0000663.1_g000003 Rmu_sc0000663.1_g000009 Rmu_sc0000663.1_g000012 Rmu_sc0000663.1_g000013 Rmu_sc0000663.1_g000021 Rmu_sc0000663.1_g000029 Rmu_sc0014328.1_g000002 Rmu_sc0014328.1_g000003 Rmu_sc0020688.1_g000001 Rmu_sc0024683.1_g000001 Rmu_sc0024683.1_g000002 Rmu_sc0033477.1_g000001 Rmu_sc0035872.1_g000001 Rmu_ssc0000010.1_g000006 Rmu_ssc0000010.1_g000008 Rmu_ssc0000010.1_g000011
rosa_roxburghii Rroxscaffold_4G00310390 Rroxscaffold_5G00360700 Rroxscaffold_5G00360710 Rroxscaffold_5G00360720 Rroxscaffold_5G00360750 Rroxscaffold_5G00360800 Rroxscaffold_5G00360820 Rroxscaffold_7G00176470
rosa_rugosa Rorug04G0145200 Rorug04G0145300 Rorug04G0145400 Rorug04G0145400 Rorug04G0145600.1 Rorug04G0145700.1 Rorug04G0148500 Rorug06G0217000
rosa_samantha Rh2BG232000 Rh4AG207900 Rh4BG205000 Rh4BG205100 Rh4CG218700 Rh4DG134800 Rh4DG204800 Rh4DG204900 Rh4DG205000 Rh4DG205100 Rh4DG205200 Rh4DG205300 Rh4DG205400 Rh4DG205500 Rh5BG434500 Rh5CG457700 Rh6AG326800 Rh6BG334300 Rh6CG340500 Rh6DG327800
rosa_wichuraiana Rw1G015270 Rw4G017570 Rw4G017580 Rw4G017590 Rw4G017600 Rw4G017610 Rw4G017620 Rw4G017630 Rw4G017640 Rw6G028420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 68, 302
AccI GTMKAC 3 cut(s) 54, 231, 675
AccII CGCG 2 cut(s) 755, 763
AciI CCGC 7 cut(s) 264, 286, 500, 603, 693, 746, 763
AclI AACGTT 1 cut(s) 475
AclWI GGATC 3 cut(s) 628, 632, 891
AcoI YGGCCR 1 cut(s) 945
AcsI RAATTY 2 cut(s) 466, 905
AcuI CTGAAG 1 cut(s) 666
AfiI CCNNNNNNNGG 5 cut(s) 124, 125, 226, 244, 316
AgsI TTSAA 3 cut(s) 609, 869, 904
AjnI CCWGG 4 cut(s) 446, 553, 688, 741
AloI GAACNNNNNNTCC 2 cut(s) 348, 380
AluBI AGCT 5 cut(s) 34, 815, 857, 862, 923
AluI AGCT 5 cut(s) 34, 815, 857, 862, 923
Alw26I GTCTC 7 cut(s) 20, 136, 310, 583, 620, 793, 905
AlwI GGATC 3 cut(s) 628, 632, 891
AoxI GGCC 1 cut(s) 945
ApeKI GCWGC 2 cut(s) 371, 392
ApoI RAATTY 2 cut(s) 466, 905
ArsI GACNNNNNNTTYG 2 cut(s) 165, 197
AspLEI GCGC 1 cut(s) 688
AsuHPI GGTGA 3 cut(s) 139, 272, 949
AxyI CCTNAGG 1 cut(s) 642
BanII GRGCYC 1 cut(s) 447
BbsI GAAGAC 1 cut(s) 387
BbvI GCAGC 2 cut(s) 358, 379
BccI CCATC 1 cut(s) 340
BceAI ACGGC 2 cut(s) 79, 400
BciT130I CCWGG 4 cut(s) 448, 555, 690, 743
BclI TGATCA 2 cut(s) 168, 711
BcoDI GTCTC 7 cut(s) 20, 136, 310, 583, 620, 793, 905
BfoI RGCGCY 1 cut(s) 689
BisI GCNGC 2 cut(s) 372, 393
BlpI GCTNAGC 1 cut(s) 858
BlsI GCNGC 2 cut(s) 373, 394
Bme1390I CCNGG 4 cut(s) 448, 555, 690, 743
BmiI GGNNCC 3 cut(s) 351, 505, 726
BmrFI CCNGG 4 cut(s) 448, 555, 690, 743
BpiI GAAGAC 1 cut(s) 387
BplI GAGNNNNNCTC 2 cut(s) 567, 599
BpmI CTGGAG 1 cut(s) 711
Bpu10I CCTNAGC 1 cut(s) 924
Bpu1102I GCTNAGC 1 cut(s) 858
BsaI GGTCTC 1 cut(s) 310
BsaJI CCNNGG 3 cut(s) 238, 315, 446
BsaWI WCCGGW 1 cut(s) 718
BsaXI ACNNNNNCTCC 2 cut(s) 348, 378
Bsc4I CCNNNNNNNGG 5 cut(s) 124, 125, 226, 244, 316
Bse21I CCTNAGG 1 cut(s) 642
Bse3DI GCAATG 2 cut(s) 9, 522
BseBI CCWGG 4 cut(s) 448, 555, 690, 743
BseDI CCNNGG 3 cut(s) 238, 315, 446
BseGI GGATG 3 cut(s) 272, 351, 659
BseLI CCNNNNNNNGG 5 cut(s) 124, 125, 226, 244, 316
BseMI GCAATG 2 cut(s) 9, 522
BseMII CTCAG 4 cut(s) 633, 774, 788, 849
BseRI GAGGAG 1 cut(s) 124
BseXI GCAGC 2 cut(s) 358, 379
BseYI CCCAGC 1 cut(s) 387
Bsh1236I CGCG 2 cut(s) 755, 763
Bsh1285I CGRYCG 1 cut(s) 336
BshFI GGCC 1 cut(s) 947
BsiEI CGRYCG 1 cut(s) 336
BsiSI CCGG 1 cut(s) 719
BslFI GGGAC 1 cut(s) 762
BslI CCNNNNNNNGG 5 cut(s) 124, 125, 226, 244, 316
BsmAI GTCTC 7 cut(s) 20, 136, 310, 583, 620, 793, 905
BsmFI GGGAC 1 cut(s) 762
BsnI GGCC 1 cut(s) 947
Bso31I GGTCTC 1 cut(s) 310
Bsp1286I GDGCHC 1 cut(s) 447
Bsp143I GATC 6 cut(s) 168, 277, 620, 637, 711, 883
Bsp1720I GCTNAGC 1 cut(s) 858
Bsp19I CCATGG 1 cut(s) 238
BspACI CCGC 7 cut(s) 264, 286, 500, 603, 693, 746, 763
BspANI GGCC 1 cut(s) 947
BspCNI CTCAG 4 cut(s) 634, 775, 789, 850
BspFNI CGCG 2 cut(s) 755, 763
BspLI GGNNCC 3 cut(s) 351, 505, 726
BspPI GGATC 3 cut(s) 628, 632, 891
BspTNI GGTCTC 1 cut(s) 310
BsrDI GCAATG 2 cut(s) 9, 522
BssECI CCNNGG 3 cut(s) 238, 315, 446
BssMI GATC 6 cut(s) 168, 277, 620, 637, 711, 883
BssNAI GTATAC 1 cut(s) 676
BssT1I CCWWGG 2 cut(s) 238, 315
Bst1107I GTATAC 1 cut(s) 676
Bst2UI CCWGG 4 cut(s) 448, 555, 690, 743
BstC8I GCNNGC 2 cut(s) 7, 551
BstDEI CTNAG 6 cut(s) 82, 642, 783, 797, 858, 924
BstDSI CCRYGG 1 cut(s) 238
BstF5I GGATG 3 cut(s) 272, 351, 659
BstFNI CGCG 2 cut(s) 755, 763
BstH2I RGCGCY 1 cut(s) 689
BstHHI GCGC 1 cut(s) 688
BstKTI GATC 6 cut(s) 171, 280, 623, 640, 714, 886
BstMAI GTCTC 7 cut(s) 20, 136, 310, 583, 620, 793, 905
BstMBI GATC 6 cut(s) 168, 277, 620, 637, 711, 883
BstMCI CGRYCG 1 cut(s) 336
BstMWI GCNNNNNNNGC 4 cut(s) 11, 512, 731, 752
BstNI CCWGG 4 cut(s) 448, 555, 690, 743
BstSCI CCNGG 4 cut(s) 446, 553, 688, 741
BstUI CGCG 2 cut(s) 755, 763
BstV1I GCAGC 2 cut(s) 358, 379
BstV2I GAAGAC 1 cut(s) 387
BstX2I RGATCY 1 cut(s) 637
BstXI CCANNNNNNTGG 1 cut(s) 943
BstYI RGATCY 1 cut(s) 637
BstZ17I GTATAC 1 cut(s) 676
Bsu36I CCTNAGG 1 cut(s) 642
BsuRI GGCC 1 cut(s) 947
BtgI CCRYGG 1 cut(s) 238
BtsCI GGATG 3 cut(s) 272, 351, 659
BtsI GCAGTG 1 cut(s) 834
BtsIMutI CAGTG 1 cut(s) 834
Cac8I GCNNGC 2 cut(s) 7, 551
CfoI GCGC 1 cut(s) 688
CseI GACGC 1 cut(s) 740
CviAII CATG 4 cut(s) 239, 461, 529, 887
DdeI CTNAG 6 cut(s) 82, 642, 783, 797, 858, 924
DpnI GATC 6 cut(s) 170, 279, 622, 639, 713, 885
DpnII GATC 6 cut(s) 168, 277, 620, 637, 711, 883
DraI TTTAAA 1 cut(s) 564
DrdI GACNNNNNNGTC 2 cut(s) 68, 302
DseDI GACNNNNNNGTC 2 cut(s) 68, 302
EaeI YGGCCR 1 cut(s) 945
EciI GGCGGA 1 cut(s) 253
Eco130I CCWWGG 2 cut(s) 238, 315
Eco24I GRGCYC 1 cut(s) 447
Eco31I GGTCTC 1 cut(s) 310
Eco57I CTGAAG 1 cut(s) 666
Eco81I CCTNAGG 1 cut(s) 642
EcoRII CCWGG 4 cut(s) 446, 553, 688, 741
EcoT14I CCWWGG 2 cut(s) 238, 315
EcoT22I ATGCAT 1 cut(s) 892
EcoT38I GRGCYC 1 cut(s) 447
ErhI CCWWGG 2 cut(s) 238, 315
FaeI CATG 4 cut(s) 242, 464, 532, 890
FalI AAGNNNNNCTT 2 cut(s) 533, 565
FaqI GGGAC 1 cut(s) 762
FatI CATG 4 cut(s) 238, 460, 528, 886
FauI CCCGC 2 cut(s) 493, 739
FauNDI CATATG 1 cut(s) 376
FbaI TGATCA 2 cut(s) 168, 711
FblI GTMKAC 3 cut(s) 54, 231, 675
Fnu4HI GCNGC 2 cut(s) 372, 393
FokI GGATG 3 cut(s) 259, 358, 666
FriOI GRGCYC 1 cut(s) 447
Fsp4HI GCNGC 2 cut(s) 372, 393
GlaI GCGC 1 cut(s) 687
GluI GCNGC 2 cut(s) 372, 393
GsaI CCCAGC 1 cut(s) 391
GsuI CTGGAG 1 cut(s) 711
HaeII RGCGCY 1 cut(s) 689
HaeIII GGCC 1 cut(s) 947
HapII CCGG 1 cut(s) 719
HgaI GACGC 1 cut(s) 740
HhaI GCGC 1 cut(s) 688
Hin1II CATG 4 cut(s) 242, 464, 532, 890
Hin6I GCGC 1 cut(s) 686
HinP1I GCGC 1 cut(s) 686
HincII GTYRAC 1 cut(s) 453
HindII GTYRAC 1 cut(s) 453
HinfI GANTC 8 cut(s) 51, 78, 129, 180, 260, 576, 750, 913
HpaII CCGG 1 cut(s) 719
HphI GGTGA 3 cut(s) 139, 272, 949
Hpy166II GTNNAC 4 cut(s) 55, 232, 453, 676
Hpy188I TCNGA 6 cut(s) 213, 277, 472, 625, 708, 784
Hpy188III TCNNGA 4 cut(s) 293, 353, 641, 728
Hpy8I GTNNAC 4 cut(s) 55, 232, 453, 676
HpyAV CCTTC 6 cut(s) 277, 535, 690, 826, 842, 869
HpyCH4IV ACGT 3 cut(s) 96, 429, 475
HpyCH4V TGCA 4 cut(s) 9, 14, 374, 890
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 512, 731, 752
HpyF3I CTNAG 6 cut(s) 82, 642, 783, 797, 858, 924
HpySE526I ACGT 3 cut(s) 96, 429, 475
Hsp92II CATG 4 cut(s) 242, 464, 532, 890
HspAI GCGC 1 cut(s) 686
Ksp22I TGATCA 2 cut(s) 168, 711
Kzo9I GATC 6 cut(s) 168, 277, 620, 637, 711, 883
LmnI GCTCC 3 cut(s) 355, 503, 730
Lsp1109I GCAGC 2 cut(s) 358, 379
MaeII ACGT 3 cut(s) 96, 429, 475
MalI GATC 6 cut(s) 170, 279, 622, 639, 713, 885
MboI GATC 6 cut(s) 168, 277, 620, 637, 711, 883
MboII GAAGA 4 cut(s) 38, 179, 392, 647
MflI RGATCY 1 cut(s) 637
MhlI GDGCHC 1 cut(s) 447
MluCI AATT 3 cut(s) 466, 615, 905
MlyI GAGTC 8 cut(s) 60, 72, 138, 189, 269, 585, 744, 907
MnlI CCTC 7 cut(s) 13, 145, 421, 538, 637, 800, 874
Mph1103I ATGCAT 1 cut(s) 892
MseI TTAA 1 cut(s) 563
MslI CAYNNNNRTG 1 cut(s) 527
MspI CCGG 1 cut(s) 719
MspR9I CCNGG 4 cut(s) 448, 555, 690, 743
MvaI CCWGG 4 cut(s) 448, 555, 690, 743
MvnI CGCG 2 cut(s) 755, 763
MwoI GCNNNNNNNGC 4 cut(s) 11, 512, 731, 752
NcoI CCATGG 1 cut(s) 238
NdeI CATATG 1 cut(s) 376
NdeII GATC 6 cut(s) 168, 277, 620, 637, 711, 883
NlaIII CATG 4 cut(s) 242, 464, 532, 890
NlaIV GGNNCC 3 cut(s) 351, 505, 726
NsiI ATGCAT 1 cut(s) 892
PcsI WCGNNNNNNNCGW 2 cut(s) 69, 426
PkrI GCNGC 2 cut(s) 373, 394
PleI GAGTC 8 cut(s) 59, 72, 137, 188, 268, 584, 744, 907
PpsI GAGTC 8 cut(s) 59, 72, 137, 188, 268, 584, 744, 907
Psp1406I AACGTT 1 cut(s) 475
Psp6I CCWGG 4 cut(s) 446, 553, 688, 741
PspFI CCCAGC 1 cut(s) 387
PspGI CCWGG 4 cut(s) 446, 553, 688, 741
PspN4I GGNNCC 3 cut(s) 351, 505, 726
PsrI GAACNNNNNNTAC 2 cut(s) 659, 691
PsuI RGATCY 1 cut(s) 637
RseI CAYNNNNRTG 1 cut(s) 527
SaqAI TTAA 1 cut(s) 563
SatI GCNGC 2 cut(s) 372, 393
Sau3AI GATC 6 cut(s) 168, 277, 620, 637, 711, 883
SchI GAGTC 8 cut(s) 60, 72, 138, 189, 269, 585, 744, 907
ScrFI CCNGG 4 cut(s) 448, 555, 690, 743
SduI GDGCHC 1 cut(s) 447
SmiMI CAYNNNNRTG 1 cut(s) 527
Sse9I AATT 3 cut(s) 466, 615, 905
SsiI CCGC 7 cut(s) 264, 286, 500, 603, 693, 746, 763
SspI AATATT 1 cut(s) 511
StyD4I CCNGG 4 cut(s) 446, 553, 688, 741
StyI CCWWGG 2 cut(s) 238, 315
TaiI ACGT 3 cut(s) 99, 432, 478
TaqI TCGA 5 cut(s) 49, 72, 111, 198, 336
TaqII GACCGA 1 cut(s) 322
TasI AATT 3 cut(s) 466, 615, 905
Tru1I TTAA 1 cut(s) 563
Tru9I TTAA 1 cut(s) 563
TscAI CASTG 1 cut(s) 834
TseI GCWGC 2 cut(s) 371, 392
TspDTI ATGAA 6 cut(s) 216, 393, 545, 807, 837, 884
TspRI CASTG 1 cut(s) 834
XapI RAATTY 2 cut(s) 466, 905
XmiI GTMKAC 3 cut(s) 54, 231, 675
Zsp2I ATGCAT 1 cut(s) 892
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.