RLG00000007946

Carboxylesterase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0000306
Physical Location & Seq
Reverse (-)
23297242 .. 23307086
9845 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007946

Sequence Viewer

Length: 4092 bp
ATGGAGCTGCCAAGGCGCCAAGATCAGAGCCAATCGGAGAGGAAGGGGCAGAAGCGGAAACTGGAAGAGGAAATCGGAGAGGAGCGAGAGATCACGGTGCAGCAGTCCGGCGAAGCCAGAAAAGCCTTACTGCTTCAGGTCGCTGAGCAGGTTCAAGTTCTCGACTCTTCTTTTTCCTGGAGCGAAGCTGATCGAAACGCTGCCAAGCGCGCCACTCACGTCCTCGCCGAGCTCGCCAAGAACGAGGACGTAGTGAACGTCATCGTTGACGGCGGTGCGGTTCCGGCTTTGGTGAAACATCTGCAAGCGCCGCCGTGCAGCGAGGTCGACCGGAGCTCTTCGAAGCACTGCGAACACGAGGTTGAGAAGGGCAGTGCCTTTGCACTAGGCCTTCTTGCCGTTAAGCCCGAGCATCAACAACTCATTGTTGACAATGGTGCATTGTCTCATCTAGTAGATTTGTTGAAGAGGCACAAGGATAGTCATGTAACTCGACCTCTGTATAGCGTCATTCGTAGAGCTGCTGATGCCATCACCAATCTCGCTCATGAAAACAGCAGCATTAAAACCCGTGTCAGGATAGAAGGTGGAATTCCACCTCTAGTTGAGTTGCTTGAATTTGCCGATACAAAGGTGCAAAGAGCAGCAGCTGGAGCGCTACGAACCCTCGCATTTAAAAATGATGAGAATAAGAATCAGATTGTTGAATGCAATGCTCTTCCTACCCTCATTTTAATGCTTCGATCTGAAGATGCTGCTATACATTACGAAGCGGTTGGTGTAATTGGAAATCTAGTTCACTCTTCTCCAAACATAAAGAAAGAGGTTCTTCTTGCGGGAGCTTTACAACCTGTTATTGGATTGCTTAGTTCCTGCTGCTTTGAGAGCCAAAGAGAGGCAGCTTTGCTACTTGGACAATTTGCTGCAACTGATTCAGATTGCAAGGTTCACATTGTACAGAGGGGTGCTGTCCGACCTTTGATTGAGATGCTTCAATCCCCAGATGTACAACTCAGGGAGATGTCAGCTTTTGCTTTGGGGAGGTTGGCACAGGACTCACACAACCAAGCTGGTATTGCTCATAATGGTGGTTTATTGCCATTGTTGAAACTTCTGGATTCAAAAAATGGGTCTCTGCAACACAATGCTGCATTTACCCTTTATGGCCTTGCAGATAATGAGGATAATGTGTCCGATTTTATTAGGGTTGGTGGTGTTCAGAAGTTGCAGGATGGAGAATTTATTTTCCAAGCTACAAAGGATTGTGTGACCAAAACATTGAAAAGATTAGAGGAGAAGATTCATGCACGAGTTTTGTCGCATTTGTTATACTTGATGCGTGTAGCAGAGAGAAATGTCCAAAGACGTGTTGCTTTGGCTTTTGCTCATCTTTGCTCCCCAGAGGACCTCAGAATCATTTTCATCGATAACAATGGTTTGGAATTGCTTCTTGGGCTTCTTGGTTCTAGTAGCGCCAAACAGCAGCTTGATGGTGCTATGGCTCTGTACAAGTTGGCCAACAAAGCCATGACTCTTTCTCCTGTGGATGCAGCTCCTCCATCTCCAACACCACAGGTTTATCTAGGGGAGCAGTATGTAAACAACCCCACGCTGTCCGATGTTACGTTTTTAGTTGAAGGTAGACGGTTCTATGCTCACAGAATTTGCCTGCTTGCTTCTTCAGATGCTTTTCGTGCAATGTTTGATGGTGGTTACCGGGAGAAGGATGCAAGGGACATCGAAATACCAAATATTAGATGGGAGGTTTTTGAGTTGATGATGAGATTTATATACACCGGATCAGTAGACATTACTTTGGATATTGCTCAAGATCTCCTCAGAGCTGCAGATCAGTATCTTTTGGAGGGACTCAAGCGGCTTTGTGAGTATTCAATAGCACAGGACATATCTTTGGAGAATGTTTCAAACATGTATGAACTTTCAGAAGCCTTCCATGCAATGTCATTAAGGCAAACGTGTATTTTGTTTATCTTGGAGCAGTTCGAAAAATTGAGTGCTAGACCAGGGCACTGTGAACTGATCAAGAATATATTGGATGAGATCAGATCTTACTTTACTAAAGCACTCACTAAGCCTAACCCACATAACTTGCGGCTAAGTTCCATAAACGCTGAGACTATGGGTTCCCTTGCCAAGGAGATAGACAGAGAGGTCATCCCATTTGTCCGTGTTTTCAAGGACGGCTCAGTAGAAAGACTACTGGGTTCTCCTTATAAAGCACCATTGCTTGATGATCCAGAATCTGGAGTCTCATCCAAAGATGTCACCATTTCGAAAAACCCTTTGATCTCAGCCAGAATCTTCCTCCCTGAACTCGACAATGAGAAGCCACACAAGAAGCTCCCCGTCTTGGTTTACTGCCATGGTGGAGGTTTCTGCATCGAGTCCGCCTTCTCTTTTGATCACCACCGGTTTCTCGACGGTTTGGTGTCCCAAGCTCAAGTTGTTGCTGTTTCTGTGGAGTACAGGATGGCTCCTGAGCACCCTCTTCCTGCCGGCTATGAAGATTGTTGGGCTGCCCTGCAATGGGTTGCTGCACACTTCATTGAAAATGGCATCAGTGAGGAGCCTTGGTTGACGAATCACGGCAACCCGGAACGTTTTTTTCTTGGTGGTGATAGTGCAGGAGCTAACATTGCTCATAATCTGGCAATGAGAGTTGGCAAAGAGGGCTTGCCTGGTGGTTTGAAGATATTGGGAACCTCTCTTACACACCCCTACTTCTGGGGTTCCAAGCCTATTGCGGAAGACCCTTGTAAAGAGCCAGAGAAGGATTTGGCAGCTATGCTTTGGAACTTTGCGTACCCCTCAGCACCTGGTGGACTTGATAATCCATTGATCAATCCTCTTGCTCCTGAAGCGCCAAGCCTGGCAGGACTAGGGTGTTCTAGGTTGTTTGTGAGCGTCTCGGAACATGATGGGCTGAAGTTTAGAGGCATTCGGTACTACGATGCCGTGAAGAAAAGTGGGTGGGAAGGAGAGGCAGAGCTGGTGGACGTGGAAGGAGAGGCTCATGCATTTCATATTTTGAAATTTGAGACTCAGAAAGCCAAGGATTTGACAAAAAAGTTGGTTGATTTTCTCAAGGTAGAAGACAAGAAGGTAGAGAAGGAGCTAGACAAAGAGCTGATCCCATTTGTCCGAGTCTACAAGGATGGCTCAGTTGAACGACTCATGGGCACTCCATATGTACCCCCAATGCTTAATGATCCAGTATCAGGAGTGTCATCAAAAGATATCACGATTTCAAAAAATCCGTTGATCTCGGCCAGAGTCTTCCTCCCAAAACTTGATGCTGATCAAACCCACCAGAAACTCCCCATCTTGGTTTACTACCATGGAGGGGCTTTTATGATCGAATCCGCCTTCTCGTTCGATCACCACAGGTATCTTGACAGCTTGGTATCCCAAGGCAAAGTTGTTGCTGTATCAGTTGAATACAGGATGGCTCCTGAGCACCCTCTTCCTGCAGGCTATGAAGATTGTTGGGCTGCCCTCCAATGGGTTGCCTCACACTTCATTGATAATGGTATCAATGAAGAGCCCTGGTTGACAAATCATGGTAATCCGGATAGGTTTTTTCTTGGTGGCGATAGTGCAGGTGCCAACATTGCTCATAATGTGGCCATGAGAGTTGGGAAAGAGGGTTTGCCTGGTGGTCTTAAAATACTGGGAACTTCTCTTACACACCCTTATTTTTGGGGCTCCAAGCCAATCGATGAATTAGACCCTTGCAAAGAGCCTGAGAAGGATTTAGGGTGTTTCGTTTGGAACTTCGCGTACCCCTCGGTACCTGGTGGGGTTGATAATCCAATGATGAATCCTTTTGGTCCAGAAGCACCAAGCTTGGCTGGACTTGGGTGTTCAAGGTTGTTTGTTAGTGTTTCTGAGAACGATACGCTGAGATATAGAGGTGTTCATTACTATGAGGCAGTGAAGAAAAGTGGTTGGAAAGGTGAAGCAGAGTTGGTTGAAGTGGAAGGAGAGGAACATGCATTTCATATTCTGAAATTTGAGACTCCGAAGGCTCAGGCTTTGACCAAAAAAGTGGCCGAGTTTCTCCTCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

1364

Amino Acids

150.88

Weight (kDa)

5.79

Isoelectric Point (pI)

40.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_ARMC5 PF24768 169 - 383 1.9e-15 ARMC5
ARM_PUB PF25598 174 - 409 3.5e-06 Plant U-box protein ARM repeat region
Arm PF00514 192 - 224 1.1e-06 Armadillo/beta-catenin-like repeat
Arm PF00514 227 - 266 4.1e-07 Armadillo/beta-catenin-like repeat
BTB PF00651 534 - 634 8.3e-25 BTB/POZ domain
COesterase PF00135 774 - 898 1.4e-09 Carboxylesterase family
BD-FAE PF20434 782 - 895 1.6e-09 BD-FAE
Abhydrolase_3 PF07859 791 - 1015 1.6e-45 alpha/beta hydrolase fold
COesterase PF00135 1099 - 1227 1.1e-08 Carboxylesterase family
BD-FAE PF20434 1108 - 1221 6.4e-10 BD-FAE
Abhydrolase_3 PF07859 1116 - 1341 2.4e-44 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000306)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19330
fragaria_vesca FvH4_2g23530 FvH4_2g23530 FvH4_2g23530 FvH4_2g23530 FvH4_2g23530 FvH4_2g23530 FvH4_4g15010 FvH4_4g15020 FvH4_4g15040 FvH4_4g15040 FvH4_4g15040 FvH4_4g15040 FvH4_4g15040 FvH4_4g15040
malus_domestica MD03G1264200.v1.1 MD03G1264700.v1.1 MD11G1284800.v1.1 MD13G1187400.v1.1 MD13G1187500.v1.1 MD13G1187900.v1.1 MD13G1188000.v1.1 MD16G1188100.v1.1 MD16G1188500.v1.1 MD16G1188600.v1.1
prunus_persica Prupe.1G154900_v2.0.a1 Prupe.1G155100_v2.0.a1 Prupe.1G155200_v2.0.a1 Prupe.1G155300_v2.0.a1 Prupe.1G155300_v2.0.a1 Prupe.8G240700_v2.0.a1
pyrus_communis pycom03g21190 pycom11g25240 pycom11g25250 pycom13g16210 pycom13g16230 pycom13g16240 pycom16g15820
rosa_chinensis RchiOBHm_Chr1g0343661 RchiOBHm_Chr4g0416821 RchiOBHm_Chr4g0416831 RchiOBHm_Chr4g0416841 RchiOBHm_Chr4g0416871 RchiOBHm_Chr4g0416951 RchiOBHm_Chr4g0416961 RchiOBHm_Chr6g0291001
rosa_laevigata RLG00000007939 RLG00000007940 RLG00000007944 RLG00000007945 RLG00000007946 RLG00000007954 RLG00000012156 RLG00000028955
rosa_multiflora Rmu_co8241865.1_g000001 Rmu_sc0000021.1_g000004 Rmu_sc0000663.1_g000003 Rmu_sc0000663.1_g000009 Rmu_sc0000663.1_g000012 Rmu_sc0000663.1_g000013 Rmu_sc0000663.1_g000021 Rmu_sc0000663.1_g000029 Rmu_sc0014328.1_g000002 Rmu_sc0014328.1_g000003 Rmu_sc0020688.1_g000001 Rmu_sc0024683.1_g000001 Rmu_sc0024683.1_g000002 Rmu_sc0033477.1_g000001 Rmu_sc0035872.1_g000001 Rmu_ssc0000010.1_g000006 Rmu_ssc0000010.1_g000008 Rmu_ssc0000010.1_g000011
rosa_roxburghii Rroxscaffold_4G00310390 Rroxscaffold_5G00360700 Rroxscaffold_5G00360710 Rroxscaffold_5G00360720 Rroxscaffold_5G00360750 Rroxscaffold_5G00360800 Rroxscaffold_5G00360820 Rroxscaffold_7G00176470
rosa_rugosa Rorug04G0145200 Rorug04G0145300 Rorug04G0145400 Rorug04G0145400 Rorug04G0145600.1 Rorug04G0145700.1 Rorug04G0148500 Rorug06G0217000
rosa_samantha Rh2BG232000 Rh4AG207900 Rh4BG205000 Rh4BG205100 Rh4CG218700 Rh4DG134800 Rh4DG204800 Rh4DG204900 Rh4DG205000 Rh4DG205100 Rh4DG205200 Rh4DG205300 Rh4DG205400 Rh4DG205500 Rh5BG434500 Rh5CG457700 Rh6AG326800 Rh6BG334300 Rh6CG340500 Rh6DG327800
rosa_wichuraiana Rw1G015270 Rw4G017570 Rw4G017580 Rw4G017590 Rw4G017600 Rw4G017610 Rw4G017620 Rw4G017630 Rw4G017640 Rw6G028420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2235
AarI CACCTGC 1 cut(s) 3611
AasI GACNNNNNNGTC 1 cut(s) 2171
Acc36I ACCTGC 2 cut(s) 139, 3611
Acc65I GGTACC 1 cut(s) 3811
AccB1I GGYRCC 3 cut(s) 15, 3623, 3811
AccB7I CCANNNNNTGG 1 cut(s) 2264
AccI GTMKAC 4 cut(s) 327, 1642, 1806, 3168
AccII CGCG 2 cut(s) 210, 3800
AccIII TCCGGA 1 cut(s) 3589
AclI AACGTT 1 cut(s) 2620
AclWI GGATC 4 cut(s) 1807, 2249, 3145, 3224
AcoI YGGCCR 4 cut(s) 1515, 3288, 3645, 4071
AcsI RAATTY 6 cut(s) 591, 617, 1238, 1662, 3053, 4031
AcuI CTGAAG 5 cut(s) 119, 768, 1665, 2898, 2966
AcyI GRCGYC 1 cut(s) 16
AdeI CACNNNGTG 1 cut(s) 2840
AfaI GTAC 9 cut(s) 957, 1008, 1508, 2486, 2825, 2966, 3213, 3803, 3813
AfeI AGCGCT 1 cut(s) 657
AflIII ACRYGT 3 cut(s) 1366, 1929, 1976
AgeI ACCGGT 1 cut(s) 2430
AjiI CACGTC 3 cut(s) 220, 1367, 3019
AjnI CCWGG 8 cut(s) 176, 2023, 2698, 2836, 2889, 3566, 3673, 3814
AjuI GAANNNNNNNTTGG 2 cut(s) 1434, 1466
AloI GAACNNNNNNTCC 2 cut(s) 780, 812
Alw21I GWGCWC 4 cut(s) 234, 338, 2505, 3480
Alw26I GTCTC 7 cut(s) 450, 1137, 2129, 2275, 2932, 3053, 4031
AlwI GGATC 4 cut(s) 1807, 2249, 3145, 3224
AlwNI CAGNNNCTG 3 cut(s) 650, 2264, 2837
Ama87I CYCGRG 1 cut(s) 407
Aor13HI TCCGGA 1 cut(s) 3589
Aor51HI AGCGCT 1 cut(s) 657
AoxI GGCC 6 cut(s) 388, 1165, 1515, 3288, 3645, 4071
ApoI RAATTY 6 cut(s) 591, 617, 1238, 1662, 3053, 4031
ArsI GACNNNNNNTTYG 2 cut(s) 1115, 1147
AsiGI ACCGGT 1 cut(s) 2430
Asp700I GAANNNNTTC 1 cut(s) 1446
Asp718I GGTACC 1 cut(s) 3811
AspLEI GCGC 7 cut(s) 18, 210, 212, 310, 658, 1475, 2884
AspS9I GGNCC 2 cut(s) 1405, 3851
AsuC2I CCSGG 2 cut(s) 1718, 2615
AsuHPI GGTGA 7 cut(s) 304, 526, 2278, 2417, 2648, 3392, 3989
AsuII TTCGAA 3 cut(s) 341, 2004, 2294
AvaI CYCGRG 1 cut(s) 407
AvaII GGWCC 2 cut(s) 1405, 3851
BaeGI GKGCMC 2 cut(s) 2031, 3203
BaeI ACNNNNGTAYC 2 cut(s) 618, 651
BalI TGGCCA 2 cut(s) 1517, 3647
BanI GGYRCC 3 cut(s) 15, 3623, 3811
BanII GRGCYC 4 cut(s) 234, 338, 3567, 3728
BauI CACGAG 2 cut(s) 356, 1308
BbsI GAAGAC 3 cut(s) 2775, 3120, 3289
Bbv12I GWGCWC 4 cut(s) 234, 338, 2505, 3480
BbvCI CCTCAGC 1 cut(s) 2830
BceAI ACGGC 6 cut(s) 286, 298, 383, 2218, 2623, 2960
BcgI CGANNNNNNTGC 2 cut(s) 307, 341
BciT130I CCWGG 8 cut(s) 178, 2025, 2700, 2838, 2891, 3568, 3675, 3816
BciVI GTATCC 1 cut(s) 3436
BclI TGATCA 4 cut(s) 2040, 2422, 2859, 3319
BcnI CCSGG 2 cut(s) 1718, 2615
BcoDI GTCTC 7 cut(s) 450, 1137, 2129, 2275, 2932, 3053, 4031
BfmI CTRYAG 2 cut(s) 1845, 3489
BfoI RGCGCY 5 cut(s) 19, 311, 659, 1476, 2885
BfuAI ACCTGC 2 cut(s) 139, 3611
BfuI GTATCC 1 cut(s) 3436
BglII AGATCT 2 cut(s) 1831, 2066
BlpI GCTNAGC 1 cut(s) 144
Bme18I GGWCC 2 cut(s) 1405, 3851
BmeT110I CYCGRG 1 cut(s) 407
BmgBI CACGTC 3 cut(s) 220, 1367, 3019
BmgT120I GGNCC 2 cut(s) 1405, 3851
BmrI ACTGGG 2 cut(s) 2231, 3701
BmuI ACTGGG 2 cut(s) 2231, 3701
BpiI GAAGAC 3 cut(s) 2775, 3120, 3289
BplI GAGNNNNNCTC 2 cut(s) 3285, 3317
BpmI CTGGAG 3 cut(s) 199, 672, 2286
Bpu10I CCTNAGC 4 cut(s) 2499, 2830, 3474, 4050
Bpu1102I GCTNAGC 1 cut(s) 144
Bpu14I TTCGAA 3 cut(s) 341, 2004, 2294
BpuEI CTTGAG 5 cut(s) 1812, 1856, 2445, 3089, 4070
BpuMI CCSGG 2 cut(s) 1718, 2615
Bsa29I ATCGAT 2 cut(s) 1425, 3738
BsaBI GATNNNNATC 2 cut(s) 1854, 3318
BsaHI GRCGYC 1 cut(s) 16
BsaI GGTCTC 1 cut(s) 1137
BsaWI WCCGGW 4 cut(s) 330, 1796, 2430, 3589
BsaXI ACNNNNNCTCC 4 cut(s) 1522, 1552, 2579, 2609
Bse118I RCCGGY 2 cut(s) 2430, 2516
Bse1I ACTGG 4 cut(s) 66, 2226, 3233, 3696
Bse3DI GCAATG 8 cut(s) 718, 1704, 1965, 2243, 2552, 2655, 2679, 3630
Bse8I GATNNNNATC 2 cut(s) 1854, 3318
BseAI TCCGGA 1 cut(s) 3589
BseBI CCWGG 8 cut(s) 178, 2025, 2700, 2838, 2891, 3568, 3675, 3816
BseCI ATCGAT 2 cut(s) 1425, 3738
BseGI GGATG 9 cut(s) 1237, 1552, 1732, 2062, 2175, 2273, 2496, 3181, 3471
BseJI GATNNNNATC 2 cut(s) 1854, 3318
BseMI GCAATG 8 cut(s) 718, 1704, 1965, 2243, 2552, 2655, 2679, 3630
BseNI ACTGG 4 cut(s) 66, 2226, 3233, 3696
BsePI GCGCGC 1 cut(s) 208
BseRI GAGGAG 6 cut(s) 95, 1307, 1545, 1826, 2600, 4073
BseSI GKGCMC 2 cut(s) 2031, 3203
BsgI GTGCAG 5 cut(s) 119, 337, 2541, 2664, 3639
Bsh1236I CGCG 2 cut(s) 210, 3800
Bsh1285I CGRYCG 1 cut(s) 331
BshFI GGCC 6 cut(s) 390, 1167, 1517, 3290, 3647, 4073
BshNI GGYRCC 3 cut(s) 15, 3623, 3811
BshTI ACCGGT 1 cut(s) 2430
BshVI ATCGAT 2 cut(s) 1425, 3738
BsiEI CGRYCG 1 cut(s) 331
BsiHKAI GWGCWC 4 cut(s) 234, 338, 2505, 3480
BsiHKCI CYCGRG 1 cut(s) 407
BsiSI CCGG 9 cut(s) 108, 284, 331, 1717, 1797, 2431, 2517, 2615, 3590
BslFI GGGAC 3 cut(s) 1748, 1881, 2437
BsmAI GTCTC 7 cut(s) 450, 1137, 2129, 2275, 2932, 3053, 4031
BsmBI CGTCTC 1 cut(s) 2932
BsmFI GGGAC 3 cut(s) 1748, 1881, 2437
BsmI GAATGC 2 cut(s) 713, 2958
BsnI GGCC 6 cut(s) 390, 1167, 1517, 3290, 3647, 4073
Bso31I GGTCTC 1 cut(s) 1137
BsoBI CYCGRG 1 cut(s) 407
Bsp119I TTCGAA 3 cut(s) 341, 2004, 2294
Bsp1286I GDGCHC 8 cut(s) 234, 338, 2031, 2505, 3203, 3480, 3567, 3728
Bsp13I TCCGGA 1 cut(s) 3589
Bsp1407I TGTACA 3 cut(s) 955, 1006, 1506
Bsp1720I GCTNAGC 1 cut(s) 144
Bsp19I CCATGG 2 cut(s) 2383, 3358
BspANI GGCC 6 cut(s) 390, 1167, 1517, 3290, 3647, 4073
BspDI ATCGAT 2 cut(s) 1425, 3738
BspEI TCCGGA 1 cut(s) 3589
BspFNI CGCG 2 cut(s) 210, 3800
BspHI TCATGA 1 cut(s) 547
BspMAI CTGCAG 2 cut(s) 1849, 3493
BspMI ACCTGC 2 cut(s) 139, 3611
BspPI GGATC 4 cut(s) 1807, 2249, 3145, 3224
BspQI GCTCTTC 3 cut(s) 343, 723, 3555
BspT104I TTCGAA 3 cut(s) 341, 2004, 2294
BspT107I GGYRCC 3 cut(s) 15, 3623, 3811
BspTNI GGTCTC 1 cut(s) 1137
BsrDI GCAATG 8 cut(s) 718, 1704, 1965, 2243, 2552, 2655, 2679, 3630
BsrFI RCCGGY 2 cut(s) 2430, 2516
BsrGI TGTACA 3 cut(s) 955, 1006, 1506
BsrI ACTGG 4 cut(s) 66, 2226, 3233, 3696
BssAI RCCGGY 2 cut(s) 2430, 2516
BssHII GCGCGC 1 cut(s) 208
BssNI GRCGYC 1 cut(s) 16
BssSI CACGAG 2 cut(s) 356, 1308
BssT1I CCWWGG 7 cut(s) 11, 2154, 2383, 2591, 3072, 3358, 3430
Bst2BI CACGAG 2 cut(s) 356, 1308
Bst2UI CCWGG 8 cut(s) 178, 2025, 2700, 2838, 2891, 3568, 3675, 3816
Bst4CI ACNGT 4 cut(s) 97, 1647, 2033, 2444
Bst6I CTCTTC 9 cut(s) 60, 172, 343, 461, 723, 808, 2514, 3489, 3555
BstACI GRCGYC 1 cut(s) 16
BstAUI TGTACA 3 cut(s) 955, 1006, 1506
BstBI TTCGAA 3 cut(s) 341, 2004, 2294
BstC8I GCNNGC 8 cut(s) 210, 234, 306, 1670, 1674, 2518, 2696, 3493
BstDSI CCRYGG 2 cut(s) 2383, 3358
BstEII GGTNACC 1 cut(s) 1712
BstENI CCTNNNNNAGG 1 cut(s) 2153
BstF5I GGATG 9 cut(s) 1237, 1552, 1732, 2062, 2175, 2273, 2496, 3181, 3471
BstFNI CGCG 2 cut(s) 210, 3800
BstH2I RGCGCY 5 cut(s) 19, 311, 659, 1476, 2885
BstHHI GCGC 7 cut(s) 18, 210, 212, 310, 658, 1475, 2884
BstMAI GTCTC 7 cut(s) 450, 1137, 2129, 2275, 2932, 3053, 4031
BstMCI CGRYCG 1 cut(s) 331
BstNI CCWGG 8 cut(s) 178, 2025, 2700, 2838, 2891, 3568, 3675, 3816
BstNSI RCATGY 2 cut(s) 1933, 4016
BstPI GGTNACC 1 cut(s) 1712
BstSFI CTRYAG 2 cut(s) 1845, 3489
BstSLI GKGCMC 2 cut(s) 2031, 3203
BstUI CGCG 2 cut(s) 210, 3800
BstV2I GAAGAC 3 cut(s) 2775, 3120, 3289
BstX2I RGATCY 2 cut(s) 1831, 2066
BstXI CCANNNNNNTGG 1 cut(s) 4069
BstYI RGATCY 2 cut(s) 1831, 2066
Bsu15I ATCGAT 2 cut(s) 1425, 3738
BsuI GTATCC 1 cut(s) 3436
BsuRI GGCC 6 cut(s) 390, 1167, 1517, 3290, 3647, 4073
BsuTUI ATCGAT 2 cut(s) 1425, 3738
BtgI CCRYGG 2 cut(s) 2383, 3358
BtrI CACGTC 3 cut(s) 220, 1367, 3019
BtsCI GGATG 9 cut(s) 1237, 1552, 1732, 2062, 2175, 2273, 2496, 3181, 3471
BtsI GCAGTG 3 cut(s) 346, 379, 3960
BtsIMutI CAGTG 5 cut(s) 346, 379, 2029, 2587, 3960
BveI ACCTGC 2 cut(s) 139, 3611
Cac8I GCNNGC 8 cut(s) 210, 234, 306, 1670, 1674, 2518, 2696, 3493
CaiI CAGNNNCTG 3 cut(s) 650, 2264, 2837
CciI TCATGA 1 cut(s) 547
CfoI GCGC 7 cut(s) 18, 210, 212, 310, 658, 1475, 2884
Cfr10I RCCGGY 2 cut(s) 2430, 2516
Cfr13I GGNCC 2 cut(s) 1405, 3851
ClaI ATCGAT 2 cut(s) 1425, 3738
CseI GACGC 2 cut(s) 496, 2914
CsiI ACCWGGT 2 cut(s) 2836, 3814
Csp6I GTAC 9 cut(s) 956, 1007, 1507, 2485, 2824, 2965, 3212, 3802, 3812
CspAI ACCGGT 1 cut(s) 2430
CspCI CAANNNNNGTGG 2 cut(s) 2091, 2126
CviQI GTAC 9 cut(s) 956, 1007, 1507, 2485, 2824, 2965, 3212, 3802, 3812
DinI GGCGCC 1 cut(s) 17
DraI TTTAAA 1 cut(s) 676
DraIII CACNNNGTG 1 cut(s) 2840
DrdI GACNNNNNNGTC 1 cut(s) 2171
DseDI GACNNNNNNGTC 1 cut(s) 2171
EaeI YGGCCR 4 cut(s) 1515, 3288, 3645, 4071
Eam1104I CTCTTC 9 cut(s) 60, 172, 343, 461, 723, 808, 2514, 3489, 3555
EarI CTCTTC 9 cut(s) 60, 172, 343, 461, 723, 808, 2514, 3489, 3555
EciI GGCGGA 2 cut(s) 2398, 3373
Ecl136II GAGCTC 2 cut(s) 232, 336
Eco130I CCWWGG 7 cut(s) 11, 2154, 2383, 2591, 3072, 3358, 3430
Eco147I AGGCCT 1 cut(s) 390
Eco24I GRGCYC 4 cut(s) 234, 338, 3567, 3728
Eco31I GGTCTC 1 cut(s) 1137
Eco32I GATATC 1 cut(s) 3259
Eco47I GGWCC 2 cut(s) 1405, 3851
Eco47III AGCGCT 1 cut(s) 657
Eco53kI GAGCTC 2 cut(s) 232, 336
Eco57I CTGAAG 5 cut(s) 119, 768, 1665, 2898, 2966
Eco88I CYCGRG 1 cut(s) 407
Eco91I GGTNACC 1 cut(s) 1712
EcoICRI GAGCTC 2 cut(s) 232, 336
EcoNI CCTNNNNNAGG 1 cut(s) 2153
EcoO109I RGGNCCY 1 cut(s) 1405
EcoO65I GGTNACC 1 cut(s) 1712
EcoRI GAATTC 1 cut(s) 591
EcoRII CCWGG 8 cut(s) 176, 2023, 2698, 2836, 2889, 3566, 3673, 3814
EcoRV GATATC 1 cut(s) 3259
EcoT14I CCWWGG 7 cut(s) 11, 2154, 2383, 2591, 3072, 3358, 3430
EcoT22I ATGCAT 2 cut(s) 3040, 4018
EcoT38I GRGCYC 4 cut(s) 234, 338, 3567, 3728
EgeI GGCGCC 1 cut(s) 17
EheI GGCGCC 1 cut(s) 17
ErhI CCWWGG 7 cut(s) 11, 2154, 2383, 2591, 3072, 3358, 3430
Esp3I CGTCTC 1 cut(s) 2932
FalI AAGNNNNNCTT 2 cut(s) 813, 845
FaqI GGGAC 3 cut(s) 1748, 1881, 2437
FauI CCCGC 1 cut(s) 829
FauNDI CATATG 1 cut(s) 3208
FbaI TGATCA 4 cut(s) 2040, 2422, 2859, 3319
FblI GTMKAC 4 cut(s) 327, 1642, 1806, 3168
FokI GGATG 9 cut(s) 1244, 1559, 1739, 2069, 2162, 2260, 2503, 3188, 3478
FriOI GRGCYC 4 cut(s) 234, 338, 3567, 3728
GlaI GCGC 7 cut(s) 17, 209, 211, 309, 657, 1474, 2883
GsuI CTGGAG 3 cut(s) 199, 672, 2286
HaeII RGCGCY 5 cut(s) 19, 311, 659, 1476, 2885
HaeIII GGCC 6 cut(s) 390, 1167, 1517, 3290, 3647, 4073
HapII CCGG 9 cut(s) 108, 284, 331, 1717, 1797, 2431, 2517, 2615, 3590
HgaI GACGC 2 cut(s) 496, 2914
HhaI GCGC 7 cut(s) 18, 210, 212, 310, 658, 1475, 2884
Hin1I GRCGYC 1 cut(s) 16
Hin6I GCGC 7 cut(s) 16, 208, 210, 308, 656, 1473, 2882
HinP1I GCGC 7 cut(s) 16, 208, 210, 308, 656, 1473, 2882
HincII GTYRAC 5 cut(s) 268, 328, 430, 2598, 3573
HindII GTYRAC 5 cut(s) 268, 328, 430, 2598, 3573
HindIII AAGCTT 1 cut(s) 3865
HpaII CCGG 9 cut(s) 108, 284, 331, 1717, 1797, 2431, 2517, 2615, 3590
HphI GGTGA 7 cut(s) 304, 526, 2278, 2417, 2648, 3392, 3989
Hpy99I CGWCG 1 cut(s) 2444
HpyCH4III ACNGT 4 cut(s) 97, 1647, 2033, 2444
HpyCH4IV ACGT 8 cut(s) 219, 249, 258, 1366, 1625, 1976, 2620, 3018
HpySE526I ACGT 8 cut(s) 219, 249, 258, 1366, 1625, 1976, 2620, 3018
Hsp92I GRCGYC 1 cut(s) 16
HspAI GCGC 7 cut(s) 16, 208, 210, 308, 656, 1473, 2882
KasI GGCGCC 1 cut(s) 15
Kpn2I TCCGGA 1 cut(s) 3589
KpnI GGTACC 1 cut(s) 3815
KroI GCCGGC 1 cut(s) 2516
KroNI GCCGGC 1 cut(s) 2518
Ksp22I TGATCA 4 cut(s) 2040, 2422, 2859, 3319
LguI GCTCTTC 3 cut(s) 343, 723, 3555
MabI ACCWGGT 2 cut(s) 2836, 3814
MaeII ACGT 8 cut(s) 219, 249, 258, 1366, 1625, 1976, 2620, 3018
MaeIII GTNAC 5 cut(s) 487, 1267, 1621, 1712, 2284
MflI RGATCY 2 cut(s) 1831, 2066
MhlI GDGCHC 8 cut(s) 234, 338, 2031, 2505, 3203, 3480, 3567, 3728
MlsI TGGCCA 2 cut(s) 1517, 3647
MluNI TGGCCA 2 cut(s) 1517, 3647
Mly113I GGCGCC 1 cut(s) 16
MmeI TCCRAC 3 cut(s) 997, 1589, 3950
Mox20I TGGCCA 2 cut(s) 1517, 3647
Mph1103I ATGCAT 2 cut(s) 3040, 4018
MroI TCCGGA 1 cut(s) 3589
MroNI GCCGGC 1 cut(s) 2516
MroXI GAANNNNTTC 1 cut(s) 1446
MscI TGGCCA 2 cut(s) 1517, 3647
MseI TTAA 7 cut(s) 402, 564, 675, 734, 1967, 3225, 3684
MslI CAYNNNNRTG 3 cut(s) 734, 1086, 3945
Msp20I TGGCCA 2 cut(s) 1517, 3647
MspA1I CMGCKG 1 cut(s) 650
MspI CCGG 9 cut(s) 108, 284, 331, 1717, 1797, 2431, 2517, 2615, 3590
Mva1269I GAATGC 2 cut(s) 713, 2958
MvaI CCWGG 8 cut(s) 178, 2025, 2700, 2838, 2891, 3568, 3675, 3816
MvnI CGCG 2 cut(s) 210, 3800
NaeI GCCGGC 1 cut(s) 2518
NarI GGCGCC 1 cut(s) 16
NciI CCSGG 2 cut(s) 1718, 2615
NcoI CCATGG 2 cut(s) 2383, 3358
NdeI CATATG 1 cut(s) 3208
NgoMIV GCCGGC 1 cut(s) 2516
NmeAIII GCCGAG 2 cut(s) 253, 3266
NmuCI GTSAC 2 cut(s) 1267, 2284
NsiI ATGCAT 2 cut(s) 3040, 4018
NspI RCATGY 2 cut(s) 1933, 4016
NspV TTCGAA 3 cut(s) 341, 2004, 2294
PagI TCATGA 1 cut(s) 547
PaqCI CACCTGC 1 cut(s) 3611
PauI GCGCGC 1 cut(s) 208
PceI AGGCCT 1 cut(s) 390
PciI ACATGT 1 cut(s) 1929
PciSI GCTCTTC 3 cut(s) 343, 723, 3555
PcsI WCGNNNNNNNCGW 3 cut(s) 225, 240, 255
PctI GAATGC 2 cut(s) 713, 2958
PdiI GCCGGC 1 cut(s) 2518
PdmI GAANNNNTTC 1 cut(s) 1446
PflMI CCANNNNNTGG 1 cut(s) 2264
PfoI TCCNGGA 1 cut(s) 176
PinAI ACCGGT 1 cut(s) 2430
PluTI GGCGCC 1 cut(s) 19
PpuMI RGGWCCY 1 cut(s) 1405
PscI ACATGT 1 cut(s) 1929
PsiI TTATAA 1 cut(s) 2235
Psp124BI GAGCTC 2 cut(s) 234, 338
Psp1406I AACGTT 1 cut(s) 2620
Psp5II RGGWCCY 1 cut(s) 1405
Psp6I CCWGG 8 cut(s) 176, 2023, 2698, 2836, 2889, 3566, 3673, 3814
PspEI GGTNACC 1 cut(s) 1712
PspGI CCWGG 8 cut(s) 176, 2023, 2698, 2836, 2889, 3566, 3673, 3814
PspPI GGNCC 2 cut(s) 1405, 3851
PspPPI RGGWCCY 1 cut(s) 1405
PsrI GAACNNNNNNTAC 8 cut(s) 2713, 2745, 2807, 2839, 3688, 3720, 3785, 3817
PstI CTGCAG 2 cut(s) 1849, 3493
PstNI CAGNNNCTG 3 cut(s) 650, 2264, 2837
PsuI RGATCY 2 cut(s) 1831, 2066
PteI GCGCGC 1 cut(s) 208
PvuII CAGCTG 1 cut(s) 650
RsaI GTAC 9 cut(s) 957, 1008, 1508, 2486, 2825, 2966, 3213, 3803, 3813
RsaNI GTAC 9 cut(s) 956, 1007, 1507, 2485, 2824, 2965, 3212, 3802, 3812
RseI CAYNNNNRTG 3 cut(s) 734, 1086, 3945
SacI GAGCTC 2 cut(s) 234, 338
SalI GTCGAC 1 cut(s) 326
SapI GCTCTTC 3 cut(s) 343, 723, 3555
SaqAI TTAA 7 cut(s) 402, 564, 675, 734, 1967, 3225, 3684
Sau96I GGNCC 2 cut(s) 1405, 3851
SbfI CCTGCAGG 1 cut(s) 3493
SdaI CCTGCAGG 1 cut(s) 3493
SduI GDGCHC 8 cut(s) 234, 338, 2031, 2505, 3203, 3480, 3567, 3728
SexAI ACCWGGT 2 cut(s) 2836, 3814
SfcI CTRYAG 2 cut(s) 1845, 3489
SfoI GGCGCC 1 cut(s) 17
SfuI TTCGAA 3 cut(s) 341, 2004, 2294
SinI GGWCC 2 cut(s) 1405, 3851
SmiMI CAYNNNNRTG 3 cut(s) 734, 1086, 3945
SmlI CTYRAG 5 cut(s) 1827, 1871, 2460, 3104, 4085
SmoI CTYRAG 5 cut(s) 1827, 1871, 2460, 3104, 4085
Sse8387I CCTGCAGG 1 cut(s) 3493
SseBI AGGCCT 1 cut(s) 390
SspDI GGCGCC 1 cut(s) 15
SspI AATATT 1 cut(s) 1753
SstI GAGCTC 2 cut(s) 234, 338
StuI AGGCCT 1 cut(s) 390
StyI CCWWGG 7 cut(s) 11, 2154, 2383, 2591, 3072, 3358, 3430
TaaI ACNGT 4 cut(s) 97, 1647, 2033, 2444
TaiI ACGT 8 cut(s) 222, 252, 261, 1369, 1628, 1979, 2623, 3021
TatI WGTACW 4 cut(s) 955, 1006, 1506, 2484
TauI GCSGC 3 cut(s) 313, 1879, 2116
Tru1I TTAA 7 cut(s) 402, 564, 675, 734, 1967, 3225, 3684
Tru9I TTAA 7 cut(s) 402, 564, 675, 734, 1967, 3225, 3684
TscAI CASTG 5 cut(s) 353, 379, 2036, 2587, 3960
TseFI GTSAC 2 cut(s) 1267, 2284
Tsp45I GTSAC 2 cut(s) 1267, 2284
TspGWI ACGGA 2 cut(s) 2177, 3267
TspRI CASTG 5 cut(s) 353, 379, 2036, 2587, 3960
Van91I CCANNNNNTGG 1 cut(s) 2264
VpaK11BI GGWCC 2 cut(s) 1405, 3851
XagI CCTNNNNNAGG 1 cut(s) 2153
XapI RAATTY 6 cut(s) 591, 617, 1238, 1662, 3053, 4031
XceI RCATGY 2 cut(s) 1933, 4016
XcmI CCANNNNNNNNNTGG 1 cut(s) 1755
XmiI GTMKAC 4 cut(s) 327, 1642, 1806, 3168
XmnI GAANNNNTTC 1 cut(s) 1446
Zsp2I ATGCAT 2 cut(s) 3040, 4018
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.