MD16G1188100.v1.1

carboxylic ester hydrolase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0000306
Physical Location & Seq
Reverse (-)
16328302 .. 16329321
1020 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1188100.v1.1.491

Sequence Viewer

Length: 1020 bp
ATGGCTTCCATCGCCAAAGAATTAGCCTTTAAGTCCTATCCTTTCATCAGAATCTTTGAAGATGGCACTGTGGAGCGCATTCCTTTTCCGTATTCTTCATATGTGCCCCCATCTCCTGATCAAGATCCAGAAACTGGCGTCTACTCCAAGGACATCACCATCTCAGACAACCCCAAATTCTCGGCTCGCCTTTTCCTCCCAAATCTCCCTCAAAACCAAACCCAAAAGCTTTCCATCTTGGTTTACTTCCATGGTGGTGCCTTTTGCATGGCGTCTACCTTCTCATTCCTTCACCAACGTTATCTCAACCGCTTGGTCTCAGAAGCCAAAGTTGTAGCTGTCTCTGTTGAGTACAGACTTGCCCCAGAGAACCCACTTCCTATTGCTTATGAAGACTGCTGGGCTGCTCTTCAATGGGTTGCTTCTCACTCAATTAACAAAGGCTCCTCAGATGGTAACAAAGAGACATGGTTACTCAACTATGGAGATTTTGACAGAGTTTACATTGGTGGTGATAGTGCAGGTGGAAATATTGCACATAACCTAGTTATGAAGGCAGGAGTTGAGGGCCTGTGTGGTGGTGTGAAAATTTTGGGGGTATTTTTGTCCTGTCCTTATTTTTGGGGTTCCAAGCCAATTGGGTCAGAGCCTAAAGGTAAAAACTTTGAAAAAACTTTACCTTATTTGGTTTGGGACTTTGTTTATCCATCAGCTCCCGGAGGGATAGACAATCCGATGGTGAATCCGGCGGGTGAGGGTGCACCGAGCCTTACTGGACTAGGGTGCTCTAAGCTGCTTGTGTGTGTTGCTGGAAAGGATCATCTGAGGGATCGAGGTGTTCAATATTATGATTTAGTGAAGGAAAGCGGGTGGAAAGGAGAATTGGAGCTGTTTGAAGTGGAAGGAGAGGATCATTGCTTTCATGTCTCGCTTGGGATCGAGACCAAGACTGATCAGACGGAGACTACGACTGAGAATGTTAAGAAAATGTTCAAACGCTTGGCATCATTTCTTGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0008150 GO:0008152 GO:0009056 GO:0016787 GO:0016788 GO:0044424 GO:0044444 GO:0044464 GO:0052689
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

340

Amino Acids

37.47

Weight (kDa)

5.58

Isoelectric Point (pI)

33.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 67 - 191 8.5e-07 Carboxylesterase family
BD-FAE PF20434 69 - 182 9.1e-10 BD-FAE
Abhydrolase_3 PF07859 80 - 308 2.2e-47 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000306)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19330
fragaria_vesca FvH4_2g23530 FvH4_2g23530 FvH4_2g23530 FvH4_2g23530 FvH4_2g23530 FvH4_2g23530 FvH4_4g15010 FvH4_4g15020 FvH4_4g15040 FvH4_4g15040 FvH4_4g15040 FvH4_4g15040 FvH4_4g15040 FvH4_4g15040
malus_domestica MD03G1264200.v1.1 MD03G1264700.v1.1 MD11G1284800.v1.1 MD13G1187400.v1.1 MD13G1187500.v1.1 MD13G1187900.v1.1 MD13G1188000.v1.1 MD16G1188100.v1.1 MD16G1188500.v1.1 MD16G1188600.v1.1
prunus_persica Prupe.1G154900_v2.0.a1 Prupe.1G155100_v2.0.a1 Prupe.1G155200_v2.0.a1 Prupe.1G155300_v2.0.a1 Prupe.1G155300_v2.0.a1 Prupe.8G240700_v2.0.a1
pyrus_communis pycom03g21190 pycom11g25240 pycom11g25250 pycom13g16210 pycom13g16230 pycom13g16240 pycom16g15820
rosa_chinensis RchiOBHm_Chr1g0343661 RchiOBHm_Chr4g0416821 RchiOBHm_Chr4g0416831 RchiOBHm_Chr4g0416841 RchiOBHm_Chr4g0416871 RchiOBHm_Chr4g0416951 RchiOBHm_Chr4g0416961 RchiOBHm_Chr6g0291001
rosa_laevigata RLG00000007939 RLG00000007940 RLG00000007944 RLG00000007945 RLG00000007946 RLG00000007954 RLG00000012156 RLG00000028955
rosa_multiflora Rmu_co8241865.1_g000001 Rmu_sc0000021.1_g000004 Rmu_sc0000663.1_g000003 Rmu_sc0000663.1_g000009 Rmu_sc0000663.1_g000012 Rmu_sc0000663.1_g000013 Rmu_sc0000663.1_g000021 Rmu_sc0000663.1_g000029 Rmu_sc0014328.1_g000002 Rmu_sc0014328.1_g000003 Rmu_sc0020688.1_g000001 Rmu_sc0024683.1_g000001 Rmu_sc0024683.1_g000002 Rmu_sc0033477.1_g000001 Rmu_sc0035872.1_g000001 Rmu_ssc0000010.1_g000006 Rmu_ssc0000010.1_g000008 Rmu_ssc0000010.1_g000011
rosa_roxburghii Rroxscaffold_4G00310390 Rroxscaffold_5G00360700 Rroxscaffold_5G00360710 Rroxscaffold_5G00360720 Rroxscaffold_5G00360750 Rroxscaffold_5G00360800 Rroxscaffold_5G00360820 Rroxscaffold_7G00176470
rosa_rugosa Rorug04G0145200 Rorug04G0145300 Rorug04G0145400 Rorug04G0145400 Rorug04G0145600.1 Rorug04G0145700.1 Rorug04G0148500 Rorug06G0217000
rosa_samantha Rh2BG232000 Rh4AG207900 Rh4BG205000 Rh4BG205100 Rh4CG218700 Rh4DG134800 Rh4DG204800 Rh4DG204900 Rh4DG205000 Rh4DG205100 Rh4DG205200 Rh4DG205300 Rh4DG205400 Rh4DG205500 Rh5BG434500 Rh5CG457700 Rh6AG326800 Rh6BG334300 Rh6CG340500 Rh6DG327800
rosa_wichuraiana Rw1G015270 Rw4G017570 Rw4G017580 Rw4G017590 Rw4G017600 Rw4G017610 Rw4G017620 Rw4G017630 Rw4G017640 Rw6G028420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 512
Acc36I ACCTGC 1 cut(s) 512
AccB1I GGYRCC 1 cut(s) 257
AccB7I CCANNNNNTGG 1 cut(s) 134
AccI GTMKAC 2 cut(s) 141, 275
AciI CCGC 3 cut(s) 310, 749, 867
AclI AACGTT 1 cut(s) 298
AclWI GGATC 5 cut(s) 119, 825, 837, 918, 944
AcsI RAATTY 2 cut(s) 176, 588
AcyI GRCGYC 2 cut(s) 138, 272
AfaI GTAC 1 cut(s) 353
AfiI CCNNNNNNNGG 1 cut(s) 134
AgsI TTSAA 6 cut(s) 59, 413, 668, 842, 896, 994
AjuI GAANNNNNNNTTGG 2 cut(s) 866, 898
AluBI AGCT 5 cut(s) 229, 338, 713, 793, 889
AluI AGCT 5 cut(s) 229, 338, 713, 793, 889
Alw21I GWGCWC 2 cut(s) 763, 788
Alw26I GTCTC 6 cut(s) 322, 346, 458, 931, 935, 956
Alw44I GTGCAC 1 cut(s) 759
AlwI GGATC 5 cut(s) 119, 825, 837, 918, 944
AlwNI CAGNNNCTG 1 cut(s) 134
AoxI GGCC 1 cut(s) 568
ApaLI GTGCAC 1 cut(s) 759
ApeKI GCWGC 2 cut(s) 404, 793
ApoI RAATTY 2 cut(s) 176, 588
Asp700I GAANNNNTTC 1 cut(s) 989
AspLEI GCGC 1 cut(s) 78
AspS9I GGNCC 1 cut(s) 568
AsuC2I CCSGG 1 cut(s) 717
AsuHPI GGTGA 5 cut(s) 148, 284, 524, 751, 764
BaeGI GKGCMC 2 cut(s) 108, 763
BanI GGYRCC 1 cut(s) 257
BbsI GAAGAC 1 cut(s) 399
Bbv12I GWGCWC 2 cut(s) 763, 788
BbvI GCAGC 2 cut(s) 391, 780
BccI CCATC 8 cut(s) 17, 56, 118, 167, 242, 446, 715, 730
BclI TGATCA 2 cut(s) 118, 952
BcnI CCSGG 1 cut(s) 717
BcoDI GTCTC 6 cut(s) 322, 346, 458, 931, 935, 956
BfaI CTAG 2 cut(s) 545, 779
BfuAI ACCTGC 1 cut(s) 512
BisI GCNGC 2 cut(s) 405, 794
BlsI GCNGC 2 cut(s) 406, 795
Bme1390I CCNGG 1 cut(s) 717
BmgT120I GGNCC 1 cut(s) 568
BmiI GGNNCC 3 cut(s) 259, 445, 628
BmrFI CCNGG 1 cut(s) 717
BmsI GCATC 1 cut(s) 1013
BpiI GAAGAC 1 cut(s) 399
BpuMI CCSGG 1 cut(s) 717
BsaBI GATNNNNATC 1 cut(s) 123
BsaHI GRCGYC 2 cut(s) 138, 272
BsaI GGTCTC 2 cut(s) 322, 935
BsaJI CCNNGG 2 cut(s) 147, 250
BsaXI ACNNNNNCTCC 2 cut(s) 428, 458
Bsc4I CCNNNNNNNGG 1 cut(s) 134
Bse1I ACTGG 2 cut(s) 139, 778
Bse3DI GCAATG 1 cut(s) 913
Bse8I GATNNNNATC 1 cut(s) 123
BseDI CCNNGG 2 cut(s) 147, 250
BseJI GATNNNNATC 1 cut(s) 123
BseLI CCNNNNNNNGG 1 cut(s) 134
BseMI GCAATG 1 cut(s) 913
BseMII CTCAG 5 cut(s) 177, 333, 462, 815, 963
BseNI ACTGG 2 cut(s) 139, 778
BseRI GAGGAG 1 cut(s) 436
BseSI GKGCMC 2 cut(s) 108, 763
BseXI GCAGC 2 cut(s) 391, 780
BseYI CCCAGC 1 cut(s) 399
BsgI GTGCAG 1 cut(s) 540
BshFI GGCC 1 cut(s) 570
BshNI GGYRCC 1 cut(s) 257
BsiHKAI GWGCWC 2 cut(s) 763, 788
BsiSI CCGG 2 cut(s) 717, 746
BslFI GGGAC 1 cut(s) 707
BslI CCNNNNNNNGG 1 cut(s) 134
BsmAI GTCTC 6 cut(s) 322, 346, 458, 931, 935, 956
BsmFI GGGAC 1 cut(s) 707
BsmI GAATGC 1 cut(s) 78
BsnI GGCC 1 cut(s) 570
Bso31I GGTCTC 2 cut(s) 322, 935
Bsp1286I GDGCHC 3 cut(s) 108, 763, 788
Bsp143I GATC 7 cut(s) 118, 124, 817, 829, 910, 936, 952
Bsp19I CCATGG 1 cut(s) 250
BspACI CCGC 3 cut(s) 310, 749, 867
BspANI GGCC 1 cut(s) 570
BspCNI CTCAG 5 cut(s) 176, 332, 461, 816, 964
BspLI GGNNCC 3 cut(s) 259, 445, 628
BspMI ACCTGC 1 cut(s) 512
BspPI GGATC 5 cut(s) 119, 825, 837, 918, 944
BspQI GCTCTTC 1 cut(s) 414
BspT107I GGYRCC 1 cut(s) 257
BspTNI GGTCTC 2 cut(s) 322, 935
BsrDI GCAATG 1 cut(s) 913
BsrI ACTGG 2 cut(s) 139, 778
BssECI CCNNGG 2 cut(s) 147, 250
BssMI GATC 7 cut(s) 118, 124, 817, 829, 910, 936, 952
BssNI GRCGYC 2 cut(s) 138, 272
BssT1I CCWWGG 2 cut(s) 147, 250
Bst4CI ACNGT 1 cut(s) 70
Bst6I CTCTTC 1 cut(s) 414
BstACI GRCGYC 2 cut(s) 138, 272
BstC8I GCNNGC 1 cut(s) 187
BstDEI CTNAG 6 cut(s) 163, 319, 448, 789, 824, 972
BstDSI CCRYGG 1 cut(s) 250
BstHHI GCGC 1 cut(s) 78
BstKTI GATC 7 cut(s) 121, 127, 820, 832, 913, 939, 955
BstMAI GTCTC 6 cut(s) 322, 346, 458, 931, 935, 956
BstMBI GATC 7 cut(s) 118, 124, 817, 829, 910, 936, 952
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstSCI CCNGG 1 cut(s) 715
BstSLI GKGCMC 2 cut(s) 108, 763
BstV1I GCAGC 2 cut(s) 391, 780
BstV2I GAAGAC 1 cut(s) 399
BstX2I RGATCY 1 cut(s) 124
BstYI RGATCY 1 cut(s) 124
BsuRI GGCC 1 cut(s) 570
BtgI CCRYGG 1 cut(s) 250
BtsIMutI CAGTG 1 cut(s) 66
BveI ACCTGC 1 cut(s) 512
Cac8I GCNNGC 1 cut(s) 187
CaiI CAGNNNCTG 1 cut(s) 134
CfoI GCGC 1 cut(s) 78
Cfr13I GGNCC 1 cut(s) 568
CseI GACGC 2 cut(s) 127, 261
Csp6I GTAC 1 cut(s) 352
CviAII CATG 4 cut(s) 251, 268, 468, 923
CviQI GTAC 1 cut(s) 352
DdeI CTNAG 6 cut(s) 163, 319, 448, 789, 824, 972
DpnI GATC 7 cut(s) 120, 126, 819, 831, 912, 938, 954
DpnII GATC 7 cut(s) 118, 124, 817, 829, 910, 936, 952
Eam1104I CTCTTC 1 cut(s) 414
EarI CTCTTC 1 cut(s) 414
Eco130I CCWWGG 2 cut(s) 147, 250
Eco31I GGTCTC 2 cut(s) 322, 935
EcoO109I RGGNCCY 1 cut(s) 568
EcoT14I CCWWGG 2 cut(s) 147, 250
ErhI CCWWGG 2 cut(s) 147, 250
FaeI CATG 4 cut(s) 254, 271, 471, 926
FaqI GGGAC 1 cut(s) 707
FatI CATG 4 cut(s) 250, 267, 467, 922
FauI CCCGC 2 cut(s) 742, 860
FauNDI CATATG 1 cut(s) 100
FbaI TGATCA 2 cut(s) 118, 952
FblI GTMKAC 2 cut(s) 141, 275
Fnu4HI GCNGC 2 cut(s) 405, 794
Fsp4HI GCNGC 2 cut(s) 405, 794
FspBI CTAG 2 cut(s) 545, 779
GlaI GCGC 1 cut(s) 77
GluI GCNGC 2 cut(s) 405, 794
GsaI CCCAGC 1 cut(s) 403
HaeIII GGCC 1 cut(s) 570
HapII CCGG 2 cut(s) 717, 746
HgaI GACGC 2 cut(s) 127, 261
HhaI GCGC 1 cut(s) 78
Hin1I GRCGYC 2 cut(s) 138, 272
Hin1II CATG 4 cut(s) 254, 271, 471, 926
Hin6I GCGC 1 cut(s) 76
HinP1I GCGC 1 cut(s) 76
HindIII AAGCTT 1 cut(s) 227
HinfI GANTC 2 cut(s) 51, 742
HpaII CCGG 2 cut(s) 717, 746
HphI GGTGA 5 cut(s) 148, 284, 524, 751, 764
Hpy166II GTNNAC 5 cut(s) 142, 244, 276, 502, 761
Hpy188I TCNGA 8 cut(s) 50, 166, 322, 451, 646, 735, 825, 957
Hpy188III TCNNGA 4 cut(s) 116, 122, 128, 940
Hpy8I GTNNAC 5 cut(s) 142, 244, 276, 502, 761
HpyAV CCTTC 5 cut(s) 289, 299, 547, 853, 896
HpyCH4III ACNGT 1 cut(s) 70
HpyCH4IV ACGT 1 cut(s) 298
HpyCH4V TGCA 4 cut(s) 267, 521, 536, 761
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpyF3I CTNAG 6 cut(s) 163, 319, 448, 789, 824, 972
HpySE526I ACGT 1 cut(s) 298
Hsp92I GRCGYC 2 cut(s) 138, 272
Hsp92II CATG 4 cut(s) 254, 271, 471, 926
HspAI GCGC 1 cut(s) 76
Ksp22I TGATCA 2 cut(s) 118, 952
Kzo9I GATC 7 cut(s) 118, 124, 817, 829, 910, 936, 952
LguI GCTCTTC 1 cut(s) 414
LmnI GCTCC 4 cut(s) 73, 449, 718, 886
Lsp1109I GCAGC 2 cut(s) 391, 780
LweI GCATC 1 cut(s) 1013
MaeI CTAG 2 cut(s) 545, 779
MaeII ACGT 1 cut(s) 298
MaeIII GTNAC 2 cut(s) 455, 471
MalI GATC 7 cut(s) 120, 126, 819, 831, 912, 938, 954
MboI GATC 7 cut(s) 118, 124, 817, 829, 910, 936, 952
MboII GAAGA 4 cut(s) 71, 87, 401, 404
MfeI CAATTG 1 cut(s) 636
MflI RGATCY 1 cut(s) 124
MhlI GDGCHC 3 cut(s) 108, 763, 788
MluCI AATT 6 cut(s) 20, 176, 432, 588, 636, 881
MnlI CCTC 9 cut(s) 206, 219, 457, 559, 713, 748, 819, 827, 901
MroXI GAANNNNTTC 1 cut(s) 989
MseI TTAA 3 cut(s) 30, 435, 981
MslI CAYNNNNRTG 1 cut(s) 255
MspI CCGG 2 cut(s) 717, 746
MspR9I CCNGG 1 cut(s) 717
MunI CAATTG 1 cut(s) 636
Mva1269I GAATGC 1 cut(s) 78
MwoI GCNNNNNNNGC 1 cut(s) 11
NciI CCSGG 1 cut(s) 717
NcoI CCATGG 1 cut(s) 250
NdeI CATATG 1 cut(s) 100
NdeII GATC 7 cut(s) 118, 124, 817, 829, 910, 936, 952
NlaIII CATG 4 cut(s) 254, 271, 471, 926
NlaIV GGNNCC 3 cut(s) 259, 445, 628
NmeAIII GCCGAG 1 cut(s) 161
PaqCI CACCTGC 1 cut(s) 512
PciSI GCTCTTC 1 cut(s) 414
PcsI WCGNNNNNNNCGW 1 cut(s) 965
PctI GAATGC 1 cut(s) 78
PdmI GAANNNNTTC 1 cut(s) 989
PfeI GAWTC 2 cut(s) 51, 742
PflMI CCANNNNNTGG 1 cut(s) 134
PfoI TCCNGGA 1 cut(s) 715
PkrI GCNGC 2 cut(s) 406, 795
Psp1406I AACGTT 1 cut(s) 298
PspFI CCCAGC 1 cut(s) 399
PspN4I GGNNCC 3 cut(s) 259, 445, 628
PspPI GGNCC 1 cut(s) 568
PstNI CAGNNNCTG 1 cut(s) 134
PsuI RGATCY 1 cut(s) 124
RsaI GTAC 1 cut(s) 353
RsaNI GTAC 1 cut(s) 352
RseI CAYNNNNRTG 1 cut(s) 255
SapI GCTCTTC 1 cut(s) 414
SaqAI TTAA 3 cut(s) 30, 435, 981
SatI GCNGC 2 cut(s) 405, 794
Sau3AI GATC 7 cut(s) 118, 124, 817, 829, 910, 936, 952
Sau96I GGNCC 1 cut(s) 568
ScrFI CCNGG 1 cut(s) 717
SduI GDGCHC 3 cut(s) 108, 763, 788
SfaNI GCATC 1 cut(s) 1013
SmiMI CAYNNNNRTG 1 cut(s) 255
Sse9I AATT 6 cut(s) 20, 176, 432, 588, 636, 881
SsiI CCGC 3 cut(s) 310, 749, 867
SspI AATATT 2 cut(s) 532, 845
SspMI CTAG 2 cut(s) 545, 779
StyD4I CCNGG 1 cut(s) 715
StyI CCWWGG 2 cut(s) 147, 250
TaaI ACNGT 1 cut(s) 70
TaiI ACGT 1 cut(s) 301
TaqI TCGA 2 cut(s) 832, 939
TasI AATT 6 cut(s) 20, 176, 432, 588, 636, 881
TatI WGTACW 1 cut(s) 351
TfiI GAWTC 2 cut(s) 51, 742
Tru1I TTAA 3 cut(s) 30, 435, 981
Tru9I TTAA 3 cut(s) 30, 435, 981
TscAI CASTG 1 cut(s) 73
TseI GCWGC 2 cut(s) 404, 793
TspDTI ATGAA 5 cut(s) 34, 87, 405, 566, 911
TspGWI ACGGA 2 cut(s) 78, 974
TspRI CASTG 1 cut(s) 73
Van91I CCANNNNNTGG 1 cut(s) 134
VneI GTGCAC 1 cut(s) 759
XapI RAATTY 2 cut(s) 176, 588
XmiI GTMKAC 2 cut(s) 141, 275
XmnI GAANNNNTTC 1 cut(s) 989
XspI CTAG 2 cut(s) 545, 779
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.