Rroxscaffold_5G00360800

Carboxylesterase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000306
Physical Location & Seq
Forward (+)
41420959 .. 41422191
1233 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00360800.1

Sequence Viewer

Length: 1233 bp
ATGTCAAACGCTTCTTCCTTTCTAGGCATATCGACAAACACCACTCGTGCACCAATCCATTATCCTATACTTCAAAAACTTCTCTCTGTCTTCTCTAATGGCTTCCACTACCAAAGAAACCGCCTCAGATTTTCTTCCTCTTATCAGAGTCTTCAAAGATCACAGAGTCATGAGCTCACCATATGTGACCCCATCTCTTCAAAACCCAGAAACCAATTTCTTCTCCAAACTCATCACCAATGCAGTCCCAGTCCCAGTGAGACTATCGATTCCGTTCCCAAGGAGATAGACACAGAGCTTCTCCCATTTCTCAGAATCTACAAAGACGGCTCTGTTGAACCACTCATGGGCTCCCCATATGTACCCCCAACACTTGATGACCCAGATTCAGGAGTGTCATCAAAAGACATCACAATTTCACAAAATCCTTTGATTTCTGCCAGACTCTTCCTCCCAAAACTCAATGAGCCTCACAAGAAAATACCCATTTTGGTTTACTACCATGGTGGAGCTTTCTGCGTCGAATCCGCCTTCTCTTTCGACCACCACCGGTTTCTCAACAGCTTGGTATCCCAAGCCAAAGTTGTTGCTGTGTCAGTCGAGTACAGAATGGTTCCTGAGCACTCTCTCCCCATTGCATATGACGATAGTTGGGCTGCACTGAATTGGGTCGCCTTGCATTTTGCTGATAATGGCATCAATGAAGAGCCCTGGTTGATCAATCACGGCGACCCGAATCGGTTATTTCTGGGTGGTGATAGTGCAGGAGCCAACATTGCTCATAATGTGGCAATGAGAGTTGGTAAAGAAGGATTGCCTAATGGTATAAATTTATTGGGAGTCTTTCTTACACACCCATTTTTTTGGGGCTCCAAGCCAATTGAGGGAGACCCCTGTGAAGAGCCTGAGAAGGATTTGGCTTGTTTGGTTTGGGACATGGCGTACCCTTCAGCCCCTGGGGGGATTGATAATCCAATGATCAATCCTGTTGCTCCAGAGGCACCAAGCTTGGCTGGACTTGGGTGTTCAAGGGTGCTTGTTGGTGTTTCTGAGAATGATGAGCTGAGGCATAGAGGTGTGGGTTACTATGAGGCAGTGAAAAGAAGTGGGTTTGAAGGTGAAGCAGAGTTGGTTGAAGTGGAAGGAGAGGATCATGCATTTCATATTTTGAAATTTGAGACTCGGAAAGCTAAGGAATTGACAAAAAAGTTGGCTGATTTCCTCCTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

410

Amino Acids

45.44

Weight (kDa)

5.7

Isoelectric Point (pI)

48.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 150 - 266 6.1e-08 Carboxylesterase family
BD-FAE PF20434 156 - 270 1.1e-08 BD-FAE
Abhydrolase_3 PF07859 164 - 388 8.4e-45 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000306)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19330
fragaria_vesca FvH4_2g23530 FvH4_2g23530 FvH4_2g23530 FvH4_2g23530 FvH4_2g23530 FvH4_2g23530 FvH4_4g15010 FvH4_4g15020 FvH4_4g15040 FvH4_4g15040 FvH4_4g15040 FvH4_4g15040 FvH4_4g15040 FvH4_4g15040
malus_domestica MD03G1264200.v1.1 MD03G1264700.v1.1 MD11G1284800.v1.1 MD13G1187400.v1.1 MD13G1187500.v1.1 MD13G1187900.v1.1 MD13G1188000.v1.1 MD16G1188100.v1.1 MD16G1188500.v1.1 MD16G1188600.v1.1
prunus_persica Prupe.1G154900_v2.0.a1 Prupe.1G155100_v2.0.a1 Prupe.1G155200_v2.0.a1 Prupe.1G155300_v2.0.a1 Prupe.1G155300_v2.0.a1 Prupe.8G240700_v2.0.a1
pyrus_communis pycom03g21190 pycom11g25240 pycom11g25250 pycom13g16210 pycom13g16230 pycom13g16240 pycom16g15820
rosa_chinensis RchiOBHm_Chr1g0343661 RchiOBHm_Chr4g0416821 RchiOBHm_Chr4g0416831 RchiOBHm_Chr4g0416841 RchiOBHm_Chr4g0416871 RchiOBHm_Chr4g0416951 RchiOBHm_Chr4g0416961 RchiOBHm_Chr6g0291001
rosa_laevigata RLG00000007939 RLG00000007940 RLG00000007944 RLG00000007945 RLG00000007946 RLG00000007954 RLG00000012156 RLG00000028955
rosa_multiflora Rmu_co8241865.1_g000001 Rmu_sc0000021.1_g000004 Rmu_sc0000663.1_g000003 Rmu_sc0000663.1_g000009 Rmu_sc0000663.1_g000012 Rmu_sc0000663.1_g000013 Rmu_sc0000663.1_g000021 Rmu_sc0000663.1_g000029 Rmu_sc0014328.1_g000002 Rmu_sc0014328.1_g000003 Rmu_sc0020688.1_g000001 Rmu_sc0024683.1_g000001 Rmu_sc0024683.1_g000002 Rmu_sc0033477.1_g000001 Rmu_sc0035872.1_g000001 Rmu_ssc0000010.1_g000006 Rmu_ssc0000010.1_g000008 Rmu_ssc0000010.1_g000011
rosa_roxburghii Rroxscaffold_4G00310390 Rroxscaffold_5G00360700 Rroxscaffold_5G00360710 Rroxscaffold_5G00360720 Rroxscaffold_5G00360750 Rroxscaffold_5G00360800 Rroxscaffold_5G00360820 Rroxscaffold_7G00176470
rosa_rugosa Rorug04G0145200 Rorug04G0145300 Rorug04G0145400 Rorug04G0145400 Rorug04G0145600.1 Rorug04G0145700.1 Rorug04G0148500 Rorug06G0217000
rosa_samantha Rh2BG232000 Rh4AG207900 Rh4BG205000 Rh4BG205100 Rh4CG218700 Rh4DG134800 Rh4DG204800 Rh4DG204900 Rh4DG205000 Rh4DG205100 Rh4DG205200 Rh4DG205300 Rh4DG205400 Rh4DG205500 Rh5BG434500 Rh5CG457700 Rh6AG326800 Rh6BG334300 Rh6CG340500 Rh6DG327800
rosa_wichuraiana Rw1G015270 Rw4G017570 Rw4G017580 Rw4G017590 Rw4G017600 Rw4G017610 Rw4G017620 Rw4G017630 Rw4G017640 Rw6G028420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1000
AciI CCGC 2 cut(s) 121, 528
AclWI GGATC 1 cut(s) 1158
AcsI RAATTY 2 cut(s) 829, 1172
AcuI CTGAAG 1 cut(s) 933
AfaI GTAC 3 cut(s) 363, 605, 944
AfiI CCNNNNNNNGG 4 cut(s) 347, 389, 884, 960
AgeI ACCGGT 1 cut(s) 549
AgsI TTSAA 8 cut(s) 74, 155, 201, 338, 1029, 1115, 1136, 1171
AjnI CCWGG 2 cut(s) 710, 955
AluBI AGCT 7 cut(s) 175, 298, 512, 564, 1008, 1063, 1190
AluI AGCT 7 cut(s) 175, 298, 512, 564, 1008, 1063, 1190
Alw21I GWGCWC 3 cut(s) 52, 177, 624
Alw26I GTCTC 3 cut(s) 254, 882, 1172
Alw44I GTGCAC 1 cut(s) 48
AlwI GGATC 1 cut(s) 1158
AlwNI CAGNNNCTG 1 cut(s) 956
ApaLI GTGCAC 1 cut(s) 48
ApeKI GCWGC 1 cut(s) 656
ApoI RAATTY 2 cut(s) 829, 1172
AsiGI ACCGGT 1 cut(s) 549
AsuHPI GGTGA 4 cut(s) 169, 227, 767, 1130
BaeGI GKGCMC 1 cut(s) 52
BanI GGYRCC 1 cut(s) 1000
BanII GRGCYC 4 cut(s) 177, 353, 711, 872
BauI CACGAG 1 cut(s) 45
BbsI GAAGAC 2 cut(s) 82, 143
Bbv12I GWGCWC 3 cut(s) 52, 177, 624
BbvCI CCTCAGC 1 cut(s) 1064
BbvI GCAGC 1 cut(s) 643
BccI CCATC 1 cut(s) 200
BceAI ACGGC 2 cut(s) 343, 742
BciT130I CCWGG 2 cut(s) 712, 957
BciVI GTATCC 1 cut(s) 580
BclI TGATCA 2 cut(s) 717, 978
BcoDI GTCTC 3 cut(s) 254, 882, 1172
BfaI CTAG 1 cut(s) 23
BfuI GTATCC 1 cut(s) 580
BisI GCNGC 1 cut(s) 657
BlsI GCNGC 1 cut(s) 658
Bme1390I CCNGG 2 cut(s) 712, 957
BmiI GGNNCC 5 cut(s) 352, 615, 769, 871, 1002
BmrFI CCNGG 2 cut(s) 712, 957
BmrI ACTGGG 2 cut(s) 243, 249
BmsI GCATC 1 cut(s) 705
BmuI ACTGGG 2 cut(s) 243, 249
BpiI GAAGAC 2 cut(s) 82, 143
BpmI CTGGAG 1 cut(s) 978
Bpu10I CCTNAGC 3 cut(s) 618, 1064, 1191
BpuEI CTTGAG 1 cut(s) 1211
Bsa29I ATCGAT 1 cut(s) 267
BsaI GGTCTC 1 cut(s) 882
BsaJI CCNNGG 5 cut(s) 279, 502, 710, 955, 956
BsaWI WCCGGW 1 cut(s) 549
BsaXI ACNNNNNCTCC 2 cut(s) 435, 465
Bsc4I CCNNNNNNNGG 4 cut(s) 347, 389, 884, 960
Bse118I RCCGGY 1 cut(s) 549
Bse1I ACTGG 2 cut(s) 249, 255
Bse3DI GCAATG 3 cut(s) 633, 774, 798
BseBI CCWGG 2 cut(s) 712, 957
BseCI ATCGAT 1 cut(s) 267
BseDI CCNNGG 5 cut(s) 279, 502, 710, 955, 956
BseLI CCNNNNNNNGG 4 cut(s) 347, 389, 884, 960
BseMI GCAATG 3 cut(s) 633, 774, 798
BseMII CTCAG 6 cut(s) 139, 325, 609, 897, 1041, 1055
BseNI ACTGG 2 cut(s) 249, 255
BseRI GAGGAG 1 cut(s) 1214
BseSI GKGCMC 1 cut(s) 52
BseXI GCAGC 1 cut(s) 643
BsgI GTGCAG 2 cut(s) 642, 783
BshNI GGYRCC 1 cut(s) 1000
BshTI ACCGGT 1 cut(s) 549
BshVI ATCGAT 1 cut(s) 267
BsiHKAI GWGCWC 3 cut(s) 52, 177, 624
BsiSI CCGG 1 cut(s) 550
BslFI GGGAC 3 cut(s) 231, 237, 947
BslI CCNNNNNNNGG 4 cut(s) 347, 389, 884, 960
BsmAI GTCTC 3 cut(s) 254, 882, 1172
BsmFI GGGAC 3 cut(s) 231, 237, 947
Bso31I GGTCTC 1 cut(s) 882
Bsp1286I GDGCHC 6 cut(s) 52, 177, 353, 624, 711, 872
Bsp143I GATC 4 cut(s) 158, 717, 978, 1150
Bsp19I CCATGG 1 cut(s) 502
BspACI CCGC 2 cut(s) 121, 528
BspCNI CTCAG 6 cut(s) 138, 324, 610, 898, 1042, 1056
BspDI ATCGAT 1 cut(s) 267
BspHI TCATGA 1 cut(s) 169
BspLI GGNNCC 5 cut(s) 352, 615, 769, 871, 1002
BspPI GGATC 1 cut(s) 1158
BspQI GCTCTTC 2 cut(s) 699, 894
BspT107I GGYRCC 1 cut(s) 1000
BspTNI GGTCTC 1 cut(s) 882
BsrDI GCAATG 3 cut(s) 633, 774, 798
BsrFI RCCGGY 1 cut(s) 549
BsrI ACTGG 2 cut(s) 249, 255
BssAI RCCGGY 1 cut(s) 549
BssECI CCNNGG 5 cut(s) 279, 502, 710, 955, 956
BssMI GATC 4 cut(s) 158, 717, 978, 1150
BssSI CACGAG 1 cut(s) 45
BssT1I CCWWGG 2 cut(s) 279, 502
Bst2BI CACGAG 1 cut(s) 45
Bst2UI CCWGG 2 cut(s) 712, 957
Bst6I CTCTTC 4 cut(s) 202, 452, 699, 894
BstDEI CTNAG 7 cut(s) 125, 311, 618, 906, 1050, 1064, 1191
BstDSI CCRYGG 1 cut(s) 502
BstKTI GATC 4 cut(s) 161, 720, 981, 1153
BstMAI GTCTC 3 cut(s) 254, 882, 1172
BstMBI GATC 4 cut(s) 158, 717, 978, 1150
BstMWI GCNNNNNNNGC 2 cut(s) 776, 998
BstNI CCWGG 2 cut(s) 712, 957
BstSCI CCNGG 2 cut(s) 710, 955
BstSLI GKGCMC 1 cut(s) 52
BstV1I GCAGC 1 cut(s) 643
BstV2I GAAGAC 2 cut(s) 82, 143
BstXI CCANNNNNNTGG 1 cut(s) 864
Bsu15I ATCGAT 1 cut(s) 267
BsuI GTATCC 1 cut(s) 580
BsuTUI ATCGAT 1 cut(s) 267
BtgI CCRYGG 1 cut(s) 502
BtsI GCAGTG 1 cut(s) 1101
BtsIMutI CAGTG 3 cut(s) 262, 659, 1101
CaiI CAGNNNCTG 1 cut(s) 956
CciI TCATGA 1 cut(s) 169
Cfr10I RCCGGY 1 cut(s) 549
ClaI ATCGAT 1 cut(s) 267
CseI GACGC 1 cut(s) 508
Csp6I GTAC 3 cut(s) 362, 604, 943
CspAI ACCGGT 1 cut(s) 549
CviAII CATG 5 cut(s) 170, 346, 503, 937, 1154
CviQI GTAC 3 cut(s) 362, 604, 943
DdeI CTNAG 7 cut(s) 125, 311, 618, 906, 1050, 1064, 1191
DpnI GATC 4 cut(s) 160, 719, 980, 1152
DpnII GATC 4 cut(s) 158, 717, 978, 1150
Eam1104I CTCTTC 4 cut(s) 202, 452, 699, 894
EarI CTCTTC 4 cut(s) 202, 452, 699, 894
EciI GGCGGA 1 cut(s) 517
Ecl136II GAGCTC 1 cut(s) 175
Eco130I CCWWGG 2 cut(s) 279, 502
Eco24I GRGCYC 4 cut(s) 177, 353, 711, 872
Eco31I GGTCTC 1 cut(s) 882
Eco53kI GAGCTC 1 cut(s) 175
Eco57I CTGAAG 1 cut(s) 933
EcoICRI GAGCTC 1 cut(s) 175
EcoRII CCWGG 2 cut(s) 710, 955
EcoT14I CCWWGG 2 cut(s) 279, 502
EcoT22I ATGCAT 1 cut(s) 1159
EcoT38I GRGCYC 4 cut(s) 177, 353, 711, 872
ErhI CCWWGG 2 cut(s) 279, 502
FaeI CATG 5 cut(s) 173, 349, 506, 940, 1157
FaqI GGGAC 3 cut(s) 231, 237, 947
FatI CATG 5 cut(s) 169, 345, 502, 936, 1153
FauNDI CATATG 3 cut(s) 182, 358, 640
FbaI TGATCA 2 cut(s) 717, 978
Fnu4HI GCNGC 1 cut(s) 657
FriOI GRGCYC 4 cut(s) 177, 353, 711, 872
Fsp4HI GCNGC 1 cut(s) 657
FspBI CTAG 1 cut(s) 23
GluI GCNGC 1 cut(s) 657
GsuI CTGGAG 1 cut(s) 978
HapII CCGG 1 cut(s) 550
HgaI GACGC 1 cut(s) 508
Hin1II CATG 5 cut(s) 173, 349, 506, 940, 1157
HindIII AAGCTT 1 cut(s) 1006
HpaII CCGG 1 cut(s) 550
HphI GGTGA 4 cut(s) 169, 227, 767, 1130
Hpy166II GTNNAC 2 cut(s) 50, 496
Hpy188I TCNGA 5 cut(s) 128, 147, 314, 1051, 1185
Hpy188III TCNNGA 4 cut(s) 170, 390, 617, 995
Hpy8I GTNNAC 2 cut(s) 50, 496
Hpy99I CGWCG 1 cut(s) 524
HpyAV CCTTC 6 cut(s) 541, 803, 904, 957, 1109, 1136
HpyCH4V TGCA 7 cut(s) 50, 243, 638, 659, 679, 764, 1157
HpyF10VI GCNNNNNNNGC 2 cut(s) 776, 998
HpyF3I CTNAG 7 cut(s) 125, 311, 618, 906, 1050, 1064, 1191
Hsp92II CATG 5 cut(s) 173, 349, 506, 940, 1157
Ksp22I TGATCA 2 cut(s) 717, 978
Kzo9I GATC 4 cut(s) 158, 717, 978, 1150
LguI GCTCTTC 2 cut(s) 699, 894
LmnI GCTCC 5 cut(s) 356, 509, 767, 875, 997
Lsp1109I GCAGC 1 cut(s) 643
LweI GCATC 1 cut(s) 705
MaeI CTAG 1 cut(s) 23
MaeIII GTNAC 2 cut(s) 185, 1082
MalI GATC 4 cut(s) 160, 719, 980, 1152
MboI GATC 4 cut(s) 158, 717, 978, 1150
MboII GAAGA 9 cut(s) 6, 82, 126, 143, 189, 212, 439, 716, 911
MfeI CAATTG 1 cut(s) 879
MhlI GDGCHC 6 cut(s) 52, 177, 353, 624, 711, 872
MluCI AATT 7 cut(s) 215, 414, 664, 829, 879, 1172, 1196
MlyI GAGTC 5 cut(s) 157, 175, 438, 849, 1174
Mph1103I ATGCAT 1 cut(s) 1159
MslI CAYNNNNRTG 1 cut(s) 1074
MspI CCGG 1 cut(s) 550
MspR9I CCNGG 2 cut(s) 712, 957
MunI CAATTG 1 cut(s) 879
MvaI CCWGG 2 cut(s) 712, 957
MwoI GCNNNNNNNGC 2 cut(s) 776, 998
NcoI CCATGG 1 cut(s) 502
NdeI CATATG 3 cut(s) 182, 358, 640
NdeII GATC 4 cut(s) 158, 717, 978, 1150
NlaIII CATG 5 cut(s) 173, 349, 506, 940, 1157
NlaIV GGNNCC 5 cut(s) 352, 615, 769, 871, 1002
NmuCI GTSAC 1 cut(s) 185
NsiI ATGCAT 1 cut(s) 1159
PagI TCATGA 1 cut(s) 169
PasI CCCWGGG 1 cut(s) 956
PciSI GCTCTTC 2 cut(s) 699, 894
PfeI GAWTC 5 cut(s) 269, 315, 386, 524, 736
PinAI ACCGGT 1 cut(s) 549
PkrI GCNGC 1 cut(s) 658
PleI GAGTC 5 cut(s) 156, 174, 438, 848, 1174
PpsI GAGTC 5 cut(s) 156, 174, 438, 848, 1174
Psp124BI GAGCTC 1 cut(s) 177
Psp6I CCWGG 2 cut(s) 710, 955
PspGI CCWGG 2 cut(s) 710, 955
PspN4I GGNNCC 5 cut(s) 352, 615, 769, 871, 1002
PstNI CAGNNNCTG 1 cut(s) 956
RsaI GTAC 3 cut(s) 363, 605, 944
RsaNI GTAC 3 cut(s) 362, 604, 943
RseI CAYNNNNRTG 1 cut(s) 1074
SacI GAGCTC 1 cut(s) 177
SapI GCTCTTC 2 cut(s) 699, 894
SatI GCNGC 1 cut(s) 657
Sau3AI GATC 4 cut(s) 158, 717, 978, 1150
SchI GAGTC 5 cut(s) 157, 175, 438, 849, 1174
ScrFI CCNGG 2 cut(s) 712, 957
SduI GDGCHC 6 cut(s) 52, 177, 353, 624, 711, 872
SetI ASST 9 cut(s) 177, 300, 514, 566, 1010, 1065, 1078, 1120, 1192
SfaNI GCATC 1 cut(s) 705
SmiMI CAYNNNNRTG 1 cut(s) 1074
SmlI CTYRAG 1 cut(s) 1226
SmoI CTYRAG 1 cut(s) 1226
Sse9I AATT 7 cut(s) 215, 414, 664, 829, 879, 1172, 1196
SsiI CCGC 2 cut(s) 121, 528
SspMI CTAG 1 cut(s) 23
SstI GAGCTC 1 cut(s) 177
StyD4I CCNGG 2 cut(s) 710, 955
StyI CCWWGG 2 cut(s) 279, 502
TaqI TCGA 5 cut(s) 32, 267, 522, 540, 600
TasI AATT 7 cut(s) 215, 414, 664, 829, 879, 1172, 1196
TatI WGTACW 1 cut(s) 603
TfiI GAWTC 5 cut(s) 269, 315, 386, 524, 736
TscAI CASTG 3 cut(s) 262, 666, 1101
TseFI GTSAC 1 cut(s) 185
TseI GCWGC 1 cut(s) 656
Tsp45I GTSAC 1 cut(s) 185
TspDTI ATGAA 2 cut(s) 717, 1151
TspGWI ACGGA 1 cut(s) 262
TspRI CASTG 3 cut(s) 262, 666, 1101
VneI GTGCAC 1 cut(s) 48
XapI RAATTY 2 cut(s) 829, 1172
XspI CTAG 1 cut(s) 23
Zsp2I ATGCAT 1 cut(s) 1159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.