FvH4_5g33571

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001117
Physical Location & Seq
Reverse (-)
24291320 .. 24292279
960 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g33571.t1

Sequence Viewer

Length: 519 bp
ATGGAGGAGTTTAAGACAGCTCTAAGCACACCAACATCTCAGGTGAAGAAAGCAAAACTTAGATGGAAACCTGCTGAGCATAATAGAAATAAACTAAATGTGGATGGTGCTTTTGTGCAAAATGCAAGCTTTGGAGGAGTGGGTGGAGTTCTCAGATCAGAAACAGGAGACTTCATAACCGCATTCAGTATACCAGTTCGACATGTGTCTAGTGCAAAGCATGTTGAGCTCTTGGCCATAAGTCAAAAAGCAGTAAGTGATATTTACAAAGCAGATGGGGACCAAGGGGAGACAATGCAGCACATACAAGGACTTAAAATCCTTTTCGCTCCTAGAACTGGCAATCAGGTTGCCAATAAGCTTTCCAGAATGACTTGCGATTCTAGACAGATGCAGAGAGTTTGGAGGCCTGCTGGCCCATTCTTGAGAGCACAAACTCTGGATGGCAAGCTTAGCCGCTGCATTTGCAAGGCCGTTAGCTTCTTGTGGAACCCAGTTCCAATGAATCCTTCTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00670 ko01100 ko01110 ko01130 ko01523 map00230 map00670 map01100 map01110 map01130 map01523
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

173

Amino Acids

19.02

Weight (kDa)

9.91

Isoelectric Point (pI)

42.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 33 - 81 5e-06 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 79
AccI GTMKAC 1 cut(s) 190
AciI CCGC 2 cut(s) 180, 457
AcoI YGGCCR 1 cut(s) 234
AfiI CCNNNNNNNGG 1 cut(s) 338
AflIII ACRYGT 1 cut(s) 202
AjuI GAANNNNNNNTTGG 1 cut(s) 493
AluBI AGCT 6 cut(s) 20, 129, 229, 361, 451, 480
AluI AGCT 6 cut(s) 20, 129, 229, 361, 451, 480
Alw21I GWGCWC 2 cut(s) 231, 433
Alw26I GTCTC 2 cut(s) 162, 284
AoxI GGCC 4 cut(s) 234, 407, 415, 471
ApeKI GCWGC 2 cut(s) 298, 459
AspS9I GGNCC 2 cut(s) 280, 416
AsuHPI GGTGA 1 cut(s) 55
AvaII GGWCC 1 cut(s) 280
BalI TGGCCA 1 cut(s) 236
BanII GRGCYC 1 cut(s) 231
Bbv12I GWGCWC 2 cut(s) 231, 433
BbvI GCAGC 2 cut(s) 310, 446
BccI CCATC 4 cut(s) 57, 98, 269, 437
BceAI ACGGC 1 cut(s) 458
BcoDI GTCTC 2 cut(s) 162, 284
BfaI CTAG 3 cut(s) 210, 333, 384
BfuAI ACCTGC 1 cut(s) 79
BisI GCNGC 3 cut(s) 299, 457, 460
BlpI GCTNAGC 2 cut(s) 75, 452
BlsI GCNGC 3 cut(s) 300, 458, 461
Bme18I GGWCC 1 cut(s) 280
BmgT120I GGNCC 2 cut(s) 280, 416
BmiI GGNNCC 2 cut(s) 281, 491
BmrI ACTGGG 1 cut(s) 488
BmsI GCATC 1 cut(s) 381
BmuI ACTGGG 1 cut(s) 488
BoxI GACNNNNGTC 1 cut(s) 205
Bpu1102I GCTNAGC 2 cut(s) 75, 452
BpuEI CTTGAG 1 cut(s) 445
BsaJI CCNNGG 1 cut(s) 283
Bsc4I CCNNNNNNNGG 1 cut(s) 338
Bse1I ACTGG 3 cut(s) 194, 343, 494
BseDI CCNNGG 1 cut(s) 283
BseGI GGATG 2 cut(s) 109, 448
BseLI CCNNNNNNNGG 1 cut(s) 338
BseMII CTCAG 3 cut(s) 53, 66, 166
BseNI ACTGG 3 cut(s) 194, 343, 494
BseRI GAGGAG 2 cut(s) 20, 150
BseXI GCAGC 2 cut(s) 310, 446
BshFI GGCC 4 cut(s) 236, 409, 417, 473
BsiHKAI GWGCWC 2 cut(s) 231, 433
BslFI GGGAC 1 cut(s) 293
BslI CCNNNNNNNGG 1 cut(s) 338
BsmAI GTCTC 2 cut(s) 162, 284
BsmFI GGGAC 1 cut(s) 293
BsmI GAATGC 1 cut(s) 182
BsnI GGCC 4 cut(s) 236, 409, 417, 473
Bsp1286I GDGCHC 2 cut(s) 231, 433
Bsp143I GATC 1 cut(s) 155
Bsp1720I GCTNAGC 2 cut(s) 75, 452
BspACI CCGC 2 cut(s) 180, 457
BspANI GGCC 4 cut(s) 236, 409, 417, 473
BspCNI CTCAG 3 cut(s) 52, 67, 165
BspLI GGNNCC 2 cut(s) 281, 491
BspMI ACCTGC 1 cut(s) 79
BsrI ACTGG 3 cut(s) 194, 343, 494
BssECI CCNNGG 1 cut(s) 283
BssMI GATC 1 cut(s) 155
BssNAI GTATAC 1 cut(s) 191
BssT1I CCWWGG 1 cut(s) 283
Bst1107I GTATAC 1 cut(s) 191
BstC8I GCNNGC 4 cut(s) 127, 411, 415, 449
BstDEI CTNAG 6 cut(s) 23, 39, 59, 75, 152, 452
BstF5I GGATG 2 cut(s) 109, 448
BstKTI GATC 1 cut(s) 158
BstMAI GTCTC 2 cut(s) 162, 284
BstMBI GATC 1 cut(s) 155
BstMWI GCNNNNNNNGC 3 cut(s) 226, 453, 465
BstNSI RCATGY 2 cut(s) 206, 224
BstPAI GACNNNNGTC 1 cut(s) 205
BstV1I GCAGC 2 cut(s) 310, 446
BstZ17I GTATAC 1 cut(s) 191
BsuRI GGCC 4 cut(s) 236, 409, 417, 473
BtsCI GGATG 2 cut(s) 109, 448
BveI ACCTGC 1 cut(s) 79
Cac8I GCNNGC 4 cut(s) 127, 411, 415, 449
Cfr13I GGNCC 2 cut(s) 280, 416
CviAII CATG 2 cut(s) 203, 221
DdeI CTNAG 6 cut(s) 23, 39, 59, 75, 152, 452
DpnI GATC 1 cut(s) 157
DpnII GATC 1 cut(s) 155
EaeI YGGCCR 1 cut(s) 234
Ecl136II GAGCTC 1 cut(s) 229
Eco130I CCWWGG 1 cut(s) 283
Eco147I AGGCCT 1 cut(s) 409
Eco24I GRGCYC 1 cut(s) 231
Eco47I GGWCC 1 cut(s) 280
Eco53kI GAGCTC 1 cut(s) 229
EcoICRI GAGCTC 1 cut(s) 229
EcoT14I CCWWGG 1 cut(s) 283
EcoT38I GRGCYC 1 cut(s) 231
ErhI CCWWGG 1 cut(s) 283
FaeI CATG 2 cut(s) 206, 224
FaiI YATR 7 cut(s) 81, 176, 191, 204, 222, 239, 305
FalI AAGNNNNNCTT 2 cut(s) 42, 74
FaqI GGGAC 1 cut(s) 293
FatI CATG 2 cut(s) 202, 220
FblI GTMKAC 1 cut(s) 190
Fnu4HI GCNGC 3 cut(s) 299, 457, 460
FokI GGATG 2 cut(s) 116, 455
FriOI GRGCYC 1 cut(s) 231
Fsp4HI GCNGC 3 cut(s) 299, 457, 460
FspBI CTAG 3 cut(s) 210, 333, 384
GluI GCNGC 3 cut(s) 299, 457, 460
HaeIII GGCC 4 cut(s) 236, 409, 417, 473
Hin1II CATG 2 cut(s) 206, 224
HindIII AAGCTT 3 cut(s) 127, 359, 449
HinfI GANTC 2 cut(s) 380, 505
HphI GGTGA 1 cut(s) 55
Hpy166II GTNNAC 1 cut(s) 191
Hpy188I TCNGA 3 cut(s) 155, 160, 514
Hpy188III TCNNGA 4 cut(s) 366, 384, 424, 440
Hpy8I GTNNAC 1 cut(s) 191
HpyAV CCTTC 1 cut(s) 519
HpyCH4V TGCA 7 cut(s) 118, 125, 215, 298, 394, 462, 468
HpyF10VI GCNNNNNNNGC 3 cut(s) 226, 453, 465
HpyF3I CTNAG 6 cut(s) 23, 39, 59, 75, 152, 452
Hsp92II CATG 2 cut(s) 206, 224
Kzo9I GATC 1 cut(s) 155
LmnI GCTCC 1 cut(s) 334
Lsp1109I GCAGC 2 cut(s) 310, 446
LweI GCATC 1 cut(s) 381
MaeI CTAG 3 cut(s) 210, 333, 384
MalI GATC 1 cut(s) 157
MboI GATC 1 cut(s) 155
MboII GAAGA 1 cut(s) 58
MhlI GDGCHC 2 cut(s) 231, 433
MlsI TGGCCA 1 cut(s) 236
MluNI TGGCCA 1 cut(s) 236
MnlI CCTC 2 cut(s) 128, 399
Mox20I TGGCCA 1 cut(s) 236
MscI TGGCCA 1 cut(s) 236
MseI TTAA 2 cut(s) 12, 315
Msp20I TGGCCA 1 cut(s) 236
MspA1I CMGCKG 1 cut(s) 459
Mva1269I GAATGC 1 cut(s) 182
MwoI GCNNNNNNNGC 3 cut(s) 226, 453, 465
NdeII GATC 1 cut(s) 155
NlaIII CATG 2 cut(s) 206, 224
NlaIV GGNNCC 2 cut(s) 281, 491
NspI RCATGY 2 cut(s) 206, 224
PceI AGGCCT 1 cut(s) 409
PciI ACATGT 1 cut(s) 202
PctI GAATGC 1 cut(s) 182
PfeI GAWTC 2 cut(s) 380, 505
PkrI GCNGC 3 cut(s) 300, 458, 461
PscI ACATGT 1 cut(s) 202
PshAI GACNNNNGTC 1 cut(s) 205
Psp124BI GAGCTC 1 cut(s) 231
PspN4I GGNNCC 2 cut(s) 281, 491
PspPI GGNCC 2 cut(s) 280, 416
SacI GAGCTC 1 cut(s) 231
SaqAI TTAA 2 cut(s) 12, 315
SatI GCNGC 3 cut(s) 299, 457, 460
Sau3AI GATC 1 cut(s) 155
Sau96I GGNCC 2 cut(s) 280, 416
SduI GDGCHC 2 cut(s) 231, 433
SetI ASST 9 cut(s) 22, 45, 73, 131, 231, 351, 363, 453, 482
SfaNI GCATC 1 cut(s) 381
SinI GGWCC 1 cut(s) 280
SmlI CTYRAG 1 cut(s) 424
SmoI CTYRAG 1 cut(s) 424
SseBI AGGCCT 1 cut(s) 409
SsiI CCGC 2 cut(s) 180, 457
SspMI CTAG 3 cut(s) 210, 333, 384
SstI GAGCTC 1 cut(s) 231
StuI AGGCCT 1 cut(s) 409
StyI CCWWGG 1 cut(s) 283
TaqI TCGA 1 cut(s) 199
TauI GCSGC 1 cut(s) 459
TfiI GAWTC 2 cut(s) 380, 505
Tru1I TTAA 2 cut(s) 12, 315
Tru9I TTAA 2 cut(s) 12, 315
TseI GCWGC 2 cut(s) 298, 459
TspDTI ATGAA 2 cut(s) 163, 518
VpaK11BI GGWCC 1 cut(s) 280
XbaI TCTAGA 1 cut(s) 383
XceI RCATGY 2 cut(s) 206, 224
XmiI GTMKAC 1 cut(s) 190
XspI CTAG 3 cut(s) 210, 333, 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.