RLG00000005499

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0001117
Physical Location & Seq
Reverse (-)
68107603 .. 68108215
613 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005499

Sequence Viewer

Length: 537 bp
ATGGATAACAGTGCAGGCCTAGGCCTGTGGTTTCTCTTCTTTCTCTCTTTGATCAATGCGAGCCCGACGGTGGAGCAGGTGGAATTGTTTGATTCTGTTGGGATGGACTGGCTGGGTTTTCTTGACCGTGGTGGTGGGTTGAGGTGTGGTACCTCTGTTGGTGTTGCTTTTGGGGCAACACAGGTTTCTGGGCAGAGCCTGGATCCGGAATCTTTGAAGGATTTGAGGTTTTGTAAGAGGCAATACGGGGAATTTCTTCCTGAGAATAGTGAAAGTCCTAGTGCGGTGGGTGTTGCCGGCAGCGATGATGATCCACCCCTGGGTCGGATCTGTGGCATTGTTATGCAGCGGCAACGTATGGCGGAGGAGGTGGTGATATGGACTTTGGTGGTTGTAGACCGGTTGACAAGGATGATCGTGGTGGCTCTACCGTCGTTGGGCGAGGTGGTCTGCGATGTCGAAGTCCTCTGTCAGCGGCGTAGGTTGTCGGTGGTGGTGGTCTGGAATTCCATGGAGAAAGCTATGGGGTGCCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

179

Amino Acids

19.4

Weight (kDa)

4.61

Isoelectric Point (pI)

41.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 67
Acc36I ACCTGC 1 cut(s) 67
Acc65I GGTACC 1 cut(s) 149
AccB1I GGYRCC 2 cut(s) 149, 528
AccI GTMKAC 1 cut(s) 396
AccIII TCCGGA 1 cut(s) 205
AciI CCGC 4 cut(s) 284, 349, 362, 475
AclWI GGATC 4 cut(s) 197, 210, 305, 335
AcsI RAATTY 2 cut(s) 251, 505
AfaI GTAC 1 cut(s) 151
AfiI CCNNNNNNNGG 5 cut(s) 70, 205, 320, 324, 437
AgeI ACCGGT 1 cut(s) 399
AgsI TTSAA 1 cut(s) 217
AjnI CCWGG 2 cut(s) 198, 318
AluBI AGCT 1 cut(s) 521
AluI AGCT 1 cut(s) 521
AlwI GGATC 4 cut(s) 197, 210, 305, 335
AlwNI CAGNNNCTG 1 cut(s) 199
Aor13HI TCCGGA 1 cut(s) 205
AoxI GGCC 2 cut(s) 16, 22
ApeKI GCWGC 2 cut(s) 300, 346
ApoI RAATTY 2 cut(s) 251, 505
AsiGI ACCGGT 1 cut(s) 399
Asp700I GAANNNNTTC 1 cut(s) 255
Asp718I GGTACC 1 cut(s) 149
AspA2I CCTAGG 1 cut(s) 19
AsuHPI GGTGA 1 cut(s) 385
AvrII CCTAGG 1 cut(s) 19
BaeGI GKGCMC 1 cut(s) 533
BaeI ACNNNNGTAYC 2 cut(s) 141, 174
BamHI GGATCC 1 cut(s) 202
BanI GGYRCC 2 cut(s) 149, 528
BanII GRGCYC 1 cut(s) 65
BbvI GCAGC 2 cut(s) 312, 358
BccI CCATC 1 cut(s) 97
BciT130I CCWGG 2 cut(s) 200, 320
BclI TGATCA 1 cut(s) 51
BfaI CTAG 2 cut(s) 20, 279
BfuAI ACCTGC 1 cut(s) 67
BisI GCNGC 4 cut(s) 301, 347, 350, 476
BlnI CCTAGG 1 cut(s) 19
BlsI GCNGC 4 cut(s) 302, 348, 351, 477
Bme1390I CCNGG 2 cut(s) 200, 320
BmiI GGNNCC 3 cut(s) 151, 204, 530
BmrFI CCNGG 2 cut(s) 200, 320
BsaBI GATNNNNATC 1 cut(s) 309
BsaJI CCNNGG 5 cut(s) 19, 127, 318, 319, 510
BsaWI WCCGGW 2 cut(s) 205, 399
Bsc4I CCNNNNNNNGG 5 cut(s) 70, 205, 320, 324, 437
Bse118I RCCGGY 2 cut(s) 296, 399
Bse1I ACTGG 1 cut(s) 113
Bse8I GATNNNNATC 1 cut(s) 309
BseAI TCCGGA 1 cut(s) 205
BseBI CCWGG 2 cut(s) 200, 320
BseDI CCNNGG 5 cut(s) 19, 127, 318, 319, 510
BseGI GGATG 2 cut(s) 108, 417
BseJI GATNNNNATC 1 cut(s) 309
BseLI CCNNNNNNNGG 5 cut(s) 70, 205, 320, 324, 437
BseMII CTCAG 1 cut(s) 252
BseNI ACTGG 1 cut(s) 113
BseRI GAGGAG 1 cut(s) 380
BseSI GKGCMC 1 cut(s) 533
BseXI GCAGC 2 cut(s) 312, 358
BseYI CCCAGC 1 cut(s) 112
BsgI GTGCAG 1 cut(s) 33
BshFI GGCC 2 cut(s) 18, 24
BshNI GGYRCC 2 cut(s) 149, 528
BshTI ACCGGT 1 cut(s) 399
BsiSI CCGG 3 cut(s) 206, 297, 400
BslI CCNNNNNNNGG 5 cut(s) 70, 205, 320, 324, 437
BsnI GGCC 2 cut(s) 18, 24
Bsp1286I GDGCHC 2 cut(s) 65, 533
Bsp13I TCCGGA 1 cut(s) 205
Bsp143I GATC 5 cut(s) 51, 202, 310, 327, 414
Bsp19I CCATGG 1 cut(s) 510
BspACI CCGC 4 cut(s) 284, 349, 362, 475
BspANI GGCC 2 cut(s) 18, 24
BspCNI CTCAG 1 cut(s) 253
BspEI TCCGGA 1 cut(s) 205
BspLI GGNNCC 3 cut(s) 151, 204, 530
BspMI ACCTGC 1 cut(s) 67
BspPI GGATC 4 cut(s) 197, 210, 305, 335
BspT107I GGYRCC 2 cut(s) 149, 528
BsrFI RCCGGY 2 cut(s) 296, 399
BsrI ACTGG 1 cut(s) 113
BssAI RCCGGY 2 cut(s) 296, 399
BssECI CCNNGG 5 cut(s) 19, 127, 318, 319, 510
BssMI GATC 5 cut(s) 51, 202, 310, 327, 414
BssT1I CCWWGG 2 cut(s) 19, 510
Bst2UI CCWGG 2 cut(s) 200, 320
Bst4CI ACNGT 4 cut(s) 11, 70, 128, 432
Bst6I CTCTTC 1 cut(s) 41
BstC8I GCNNGC 3 cut(s) 16, 61, 298
BstDEI CTNAG 2 cut(s) 261, 534
BstDSI CCRYGG 2 cut(s) 127, 510
BstF5I GGATG 2 cut(s) 108, 417
BstKTI GATC 5 cut(s) 54, 205, 313, 330, 417
BstMBI GATC 5 cut(s) 51, 202, 310, 327, 414
BstMWI GCNNNNNNNGC 1 cut(s) 173
BstNI CCWGG 2 cut(s) 200, 320
BstSCI CCNGG 2 cut(s) 198, 318
BstSLI GKGCMC 1 cut(s) 533
BstV1I GCAGC 2 cut(s) 312, 358
BstX2I RGATCY 2 cut(s) 202, 327
BstYI RGATCY 2 cut(s) 202, 327
BsuRI GGCC 2 cut(s) 18, 24
BtgI CCRYGG 2 cut(s) 127, 510
BtgZI GCGATG 2 cut(s) 318, 468
BtsCI GGATG 2 cut(s) 108, 417
BtsIMutI CAGTG 1 cut(s) 16
BveI ACCTGC 1 cut(s) 67
Cac8I GCNNGC 3 cut(s) 16, 61, 298
CaiI CAGNNNCTG 1 cut(s) 199
Cfr10I RCCGGY 2 cut(s) 296, 399
Csp6I GTAC 1 cut(s) 150
CspAI ACCGGT 1 cut(s) 399
CviAII CATG 1 cut(s) 511
CviJI RGCY 7 cut(s) 18, 24, 63, 112, 198, 425, 521
CviKI_1 RGCY 7 cut(s) 18, 24, 63, 112, 198, 425, 521
CviQI GTAC 1 cut(s) 150
DdeI CTNAG 2 cut(s) 261, 534
DpnI GATC 5 cut(s) 53, 204, 312, 329, 416
DpnII GATC 5 cut(s) 51, 202, 310, 327, 414
Eam1104I CTCTTC 1 cut(s) 41
EarI CTCTTC 1 cut(s) 41
EciI GGCGGA 1 cut(s) 377
Eco130I CCWWGG 2 cut(s) 19, 510
Eco147I AGGCCT 2 cut(s) 18, 24
Eco24I GRGCYC 1 cut(s) 65
EcoRI GAATTC 1 cut(s) 505
EcoRII CCWGG 2 cut(s) 198, 318
EcoT14I CCWWGG 2 cut(s) 19, 510
EcoT38I GRGCYC 1 cut(s) 65
ErhI CCWWGG 2 cut(s) 19, 510
FaeI CATG 1 cut(s) 514
FaiI YATR 5 cut(s) 344, 359, 379, 512, 524
FatI CATG 1 cut(s) 510
FbaI TGATCA 1 cut(s) 51
FblI GTMKAC 1 cut(s) 396
Fnu4HI GCNGC 4 cut(s) 301, 347, 350, 476
FokI GGATG 2 cut(s) 115, 424
FriOI GRGCYC 1 cut(s) 65
Fsp4HI GCNGC 4 cut(s) 301, 347, 350, 476
FspBI CTAG 2 cut(s) 20, 279
GluI GCNGC 4 cut(s) 301, 347, 350, 476
GsaI CCCAGC 1 cut(s) 116
HaeIII GGCC 2 cut(s) 18, 24
HapII CCGG 3 cut(s) 206, 297, 400
Hin1II CATG 1 cut(s) 514
HincII GTYRAC 1 cut(s) 405
HindII GTYRAC 1 cut(s) 405
HinfI GANTC 2 cut(s) 92, 209
HpaII CCGG 3 cut(s) 206, 297, 400
HphI GGTGA 1 cut(s) 385
Hpy166II GTNNAC 2 cut(s) 397, 405
Hpy188I TCNGA 1 cut(s) 327
Hpy188III TCNNGA 4 cut(s) 122, 206, 260, 502
Hpy8I GTNNAC 2 cut(s) 397, 405
Hpy99I CGWCG 2 cut(s) 70, 436
HpyAV CCTTC 1 cut(s) 211
HpyCH4III ACNGT 4 cut(s) 11, 70, 128, 432
HpyCH4IV ACGT 1 cut(s) 355
HpyCH4V TGCA 2 cut(s) 14, 346
HpyF10VI GCNNNNNNNGC 1 cut(s) 173
HpyF3I CTNAG 2 cut(s) 261, 534
HpySE526I ACGT 1 cut(s) 355
Hsp92II CATG 1 cut(s) 514
Kpn2I TCCGGA 1 cut(s) 205
KpnI GGTACC 1 cut(s) 153
KroI GCCGGC 1 cut(s) 296
KroNI GCCGGC 1 cut(s) 298
Ksp22I TGATCA 1 cut(s) 51
Kzo9I GATC 5 cut(s) 51, 202, 310, 327, 414
LmnI GCTCC 1 cut(s) 73
Lsp1109I GCAGC 2 cut(s) 312, 358
MaeI CTAG 2 cut(s) 20, 279
MaeII ACGT 1 cut(s) 355
MalI GATC 5 cut(s) 53, 204, 312, 329, 416
MboI GATC 5 cut(s) 51, 202, 310, 327, 414
MboII GAAGA 2 cut(s) 28, 248
MflI RGATCY 2 cut(s) 202, 327
MhlI GDGCHC 2 cut(s) 65, 533
MluCI AATT 3 cut(s) 83, 251, 505
MmeI TCCRAC 1 cut(s) 305
MnlI CCTC 8 cut(s) 135, 163, 219, 231, 358, 361, 436, 476
MroI TCCGGA 1 cut(s) 205
MroNI GCCGGC 1 cut(s) 296
MroXI GAANNNNTTC 1 cut(s) 255
MslI CAYNNNNRTG 1 cut(s) 341
MspA1I CMGCKG 2 cut(s) 349, 475
MspI CCGG 3 cut(s) 206, 297, 400
MspR9I CCNGG 2 cut(s) 200, 320
MvaI CCWGG 2 cut(s) 200, 320
MwoI GCNNNNNNNGC 1 cut(s) 173
NaeI GCCGGC 1 cut(s) 298
NcoI CCATGG 1 cut(s) 510
NdeII GATC 5 cut(s) 51, 202, 310, 327, 414
NgoMIV GCCGGC 1 cut(s) 296
NlaIII CATG 1 cut(s) 514
NlaIV GGNNCC 3 cut(s) 151, 204, 530
PaqCI CACCTGC 1 cut(s) 67
PasI CCCWGGG 1 cut(s) 319
PceI AGGCCT 2 cut(s) 18, 24
PdiI GCCGGC 1 cut(s) 298
PdmI GAANNNNTTC 1 cut(s) 255
PfeI GAWTC 2 cut(s) 92, 209
PinAI ACCGGT 1 cut(s) 399
PkrI GCNGC 4 cut(s) 302, 348, 351, 477
Psp6I CCWGG 2 cut(s) 198, 318
PspFI CCCAGC 1 cut(s) 112
PspGI CCWGG 2 cut(s) 198, 318
PspN4I GGNNCC 3 cut(s) 151, 204, 530
PstNI CAGNNNCTG 1 cut(s) 199
PsuI RGATCY 2 cut(s) 202, 327
RsaI GTAC 1 cut(s) 151
RsaNI GTAC 1 cut(s) 150
RseI CAYNNNNRTG 1 cut(s) 341
SatI GCNGC 4 cut(s) 301, 347, 350, 476
Sau3AI GATC 5 cut(s) 51, 202, 310, 327, 414
ScrFI CCNGG 2 cut(s) 200, 320
SduI GDGCHC 2 cut(s) 65, 533
SmiMI CAYNNNNRTG 1 cut(s) 341
Sse9I AATT 3 cut(s) 83, 251, 505
SseBI AGGCCT 2 cut(s) 18, 24
SsiI CCGC 4 cut(s) 284, 349, 362, 475
SspMI CTAG 2 cut(s) 20, 279
StuI AGGCCT 2 cut(s) 18, 24
StyD4I CCNGG 2 cut(s) 198, 318
StyI CCWWGG 2 cut(s) 19, 510
TaaI ACNGT 4 cut(s) 11, 70, 128, 432
TaiI ACGT 1 cut(s) 358
TaqI TCGA 1 cut(s) 459
TasI AATT 3 cut(s) 83, 251, 505
TauI GCSGC 2 cut(s) 352, 478
TfiI GAWTC 2 cut(s) 92, 209
TscAI CASTG 1 cut(s) 16
TseI GCWGC 2 cut(s) 300, 346
TspRI CASTG 1 cut(s) 16
XapI RAATTY 2 cut(s) 251, 505
XmaJI CCTAGG 1 cut(s) 19
XmiI GTMKAC 1 cut(s) 396
XmnI GAANNNNTTC 1 cut(s) 255
XspI CTAG 2 cut(s) 20, 279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.