Rh1DG081500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0001117
Physical Location & Seq
Forward (+)
14938372 .. 14938903
532 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG081500.1

Sequence Viewer

Length: 378 bp
ATGGATAACAGTGCAGGCCTAGGCATGTGGTTTCTCTTCTTTCTCTATTTGACTGGTGCAAGCTCGACAGTGGAGTGGGCGGAGCTTTCTGATTATGTTGGGATGGAATGGCTAGGTGTATTTGACTGCAGTGGTGGGTTGAGTTGTGATACCTCTGTCGGTGTTGCTTTTGGGGCAACACAAGTTTCTAGGTGGAGCCTGGATCCGGAATCTTTGACAGATCTGAGGTTTTGTAATAGGCGATACTGGGAATTTCTTCCTGAGAATATCGAAGATCCTTGTGCGTTGGGTCTTGCCAGCAGCGATGGTGATCAGCCCTTGGGGTCTAGGGCAATCCTGATGACTGGGTTAACACGAGAAGAGAATGGCCGGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

125

Amino Acids

13.78

Weight (kDa)

4.07

Isoelectric Point (pI)

40.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 205
AciI CCGC 1 cut(s) 80
AclWI GGATC 3 cut(s) 197, 210, 269
AcoI YGGCCR 1 cut(s) 367
AcsI RAATTY 1 cut(s) 251
AfiI CCNNNNNNNGG 1 cut(s) 205
AjnI CCWGG 1 cut(s) 198
AluBI AGCT 2 cut(s) 63, 85
AluI AGCT 2 cut(s) 63, 85
AlwI GGATC 3 cut(s) 197, 210, 269
Aor13HI TCCGGA 1 cut(s) 205
AoxI GGCC 2 cut(s) 16, 367
ApeKI GCWGC 1 cut(s) 300
ApoI RAATTY 1 cut(s) 251
Asp700I GAANNNNTTC 1 cut(s) 255
AspA2I CCTAGG 1 cut(s) 19
AsuHPI GGTGA 1 cut(s) 320
AvrII CCTAGG 1 cut(s) 19
BaeI ACNNNNGTAYC 2 cut(s) 141, 174
BamHI GGATCC 1 cut(s) 202
BauI CACGAG 1 cut(s) 354
BbvI GCAGC 1 cut(s) 312
BccI CCATC 2 cut(s) 97, 299
BciT130I CCWGG 1 cut(s) 200
BclI TGATCA 1 cut(s) 310
BfaI CTAG 4 cut(s) 20, 113, 189, 327
BfmI CTRYAG 1 cut(s) 127
BglII AGATCT 1 cut(s) 220
BisI GCNGC 1 cut(s) 301
BlnI CCTAGG 1 cut(s) 19
BlsI GCNGC 1 cut(s) 302
Bme1390I CCNGG 1 cut(s) 200
BmiI GGNNCC 2 cut(s) 197, 204
BmrFI CCNGG 1 cut(s) 200
BmrI ACTGGG 2 cut(s) 256, 354
BmuI ACTGGG 2 cut(s) 256, 354
BsaBI GATNNNNATC 1 cut(s) 309
BsaJI CCNNGG 2 cut(s) 19, 318
BsaWI WCCGGW 1 cut(s) 205
Bsc4I CCNNNNNNNGG 1 cut(s) 205
Bse118I RCCGGY 1 cut(s) 369
Bse1I ACTGG 3 cut(s) 58, 251, 349
Bse8I GATNNNNATC 1 cut(s) 309
BseAI TCCGGA 1 cut(s) 205
BseBI CCWGG 1 cut(s) 200
BseDI CCNNGG 2 cut(s) 19, 318
BseGI GGATG 1 cut(s) 108
BseJI GATNNNNATC 1 cut(s) 309
BseLI CCNNNNNNNGG 1 cut(s) 205
BseMII CTCAG 2 cut(s) 215, 252
BseNI ACTGG 3 cut(s) 58, 251, 349
BseXI GCAGC 1 cut(s) 312
BsgI GTGCAG 1 cut(s) 33
BshFI GGCC 2 cut(s) 18, 369
BsiSI CCGG 2 cut(s) 206, 370
BslI CCNNNNNNNGG 1 cut(s) 205
BsnI GGCC 2 cut(s) 18, 369
Bsp13I TCCGGA 1 cut(s) 205
Bsp143I GATC 4 cut(s) 202, 220, 274, 310
BspACI CCGC 1 cut(s) 80
BspANI GGCC 2 cut(s) 18, 369
BspCNI CTCAG 2 cut(s) 216, 253
BspEI TCCGGA 1 cut(s) 205
BspLI GGNNCC 2 cut(s) 197, 204
BspMAI CTGCAG 1 cut(s) 131
BspPI GGATC 3 cut(s) 197, 210, 269
BsrFI RCCGGY 1 cut(s) 369
BsrI ACTGG 3 cut(s) 58, 251, 349
BssAI RCCGGY 1 cut(s) 369
BssECI CCNNGG 2 cut(s) 19, 318
BssMI GATC 4 cut(s) 202, 220, 274, 310
BssSI CACGAG 1 cut(s) 354
BssT1I CCWWGG 2 cut(s) 19, 318
Bst2BI CACGAG 1 cut(s) 354
Bst2UI CCWGG 1 cut(s) 200
Bst4CI ACNGT 2 cut(s) 11, 70
Bst6I CTCTTC 2 cut(s) 41, 354
BstC8I GCNNGC 3 cut(s) 16, 61, 298
BstDEI CTNAG 2 cut(s) 224, 261
BstF5I GGATG 1 cut(s) 108
BstKTI GATC 4 cut(s) 205, 223, 277, 313
BstMBI GATC 4 cut(s) 202, 220, 274, 310
BstMWI GCNNNNNNNGC 1 cut(s) 173
BstNI CCWGG 1 cut(s) 200
BstNSI RCATGY 1 cut(s) 28
BstSCI CCNGG 1 cut(s) 198
BstSFI CTRYAG 1 cut(s) 127
BstV1I GCAGC 1 cut(s) 312
BstX2I RGATCY 3 cut(s) 202, 220, 274
BstYI RGATCY 3 cut(s) 202, 220, 274
BsuRI GGCC 2 cut(s) 18, 369
BtgZI GCGATG 1 cut(s) 318
BtsCI GGATG 1 cut(s) 108
BtsI GCAGTG 1 cut(s) 136
BtsIMutI CAGTG 3 cut(s) 16, 75, 136
Cac8I GCNNGC 3 cut(s) 16, 61, 298
Cfr10I RCCGGY 1 cut(s) 369
CviAII CATG 1 cut(s) 25
CviJI RGCY 7 cut(s) 18, 63, 85, 112, 198, 316, 369
CviKI_1 RGCY 7 cut(s) 18, 63, 85, 112, 198, 316, 369
DdeI CTNAG 2 cut(s) 224, 261
DpnI GATC 4 cut(s) 204, 222, 276, 312
DpnII GATC 4 cut(s) 202, 220, 274, 310
EaeI YGGCCR 1 cut(s) 367
Eam1104I CTCTTC 2 cut(s) 41, 354
EarI CTCTTC 2 cut(s) 41, 354
EciI GGCGGA 1 cut(s) 95
Eco130I CCWWGG 2 cut(s) 19, 318
Eco147I AGGCCT 1 cut(s) 18
EcoRII CCWGG 1 cut(s) 198
EcoT14I CCWWGG 2 cut(s) 19, 318
ErhI CCWWGG 2 cut(s) 19, 318
FaeI CATG 1 cut(s) 28
FaiI YATR 2 cut(s) 26, 96
FatI CATG 1 cut(s) 24
FbaI TGATCA 1 cut(s) 310
Fnu4HI GCNGC 1 cut(s) 301
FokI GGATG 1 cut(s) 115
Fsp4HI GCNGC 1 cut(s) 301
FspBI CTAG 4 cut(s) 20, 113, 189, 327
GluI GCNGC 1 cut(s) 301
HaeIII GGCC 2 cut(s) 18, 369
HapII CCGG 2 cut(s) 206, 370
Hin1II CATG 1 cut(s) 28
HincII GTYRAC 1 cut(s) 351
HindII GTYRAC 1 cut(s) 351
HinfI GANTC 1 cut(s) 209
HpaI GTTAAC 1 cut(s) 351
HpaII CCGG 2 cut(s) 206, 370
HphI GGTGA 1 cut(s) 320
Hpy166II GTNNAC 1 cut(s) 351
Hpy188I TCNGA 2 cut(s) 91, 225
Hpy188III TCNNGA 3 cut(s) 206, 260, 337
Hpy8I GTNNAC 1 cut(s) 351
HpyCH4III ACNGT 2 cut(s) 11, 70
HpyCH4V TGCA 3 cut(s) 14, 59, 129
HpyF10VI GCNNNNNNNGC 1 cut(s) 173
HpyF3I CTNAG 2 cut(s) 224, 261
Hsp92II CATG 1 cut(s) 28
Kpn2I TCCGGA 1 cut(s) 205
Ksp22I TGATCA 1 cut(s) 310
KspAI GTTAAC 1 cut(s) 351
Kzo9I GATC 4 cut(s) 202, 220, 274, 310
LmnI GCTCC 2 cut(s) 82, 195
LpnPI CCDG 9 cut(s) 39, 185, 212, 219, 232, 273, 310, 330, 350
Lsp1109I GCAGC 1 cut(s) 312
MaeI CTAG 4 cut(s) 20, 113, 189, 327
MalI GATC 4 cut(s) 204, 222, 276, 312
MboI GATC 4 cut(s) 202, 220, 274, 310
MboII GAAGA 4 cut(s) 28, 248, 284, 371
MflI RGATCY 3 cut(s) 202, 220, 274
MluCI AATT 1 cut(s) 251
MnlI CCTC 2 cut(s) 163, 219
MroI TCCGGA 1 cut(s) 205
MroXI GAANNNNTTC 1 cut(s) 255
MseI TTAA 1 cut(s) 350
MspI CCGG 2 cut(s) 206, 370
MspR9I CCNGG 1 cut(s) 200
MvaI CCWGG 1 cut(s) 200
MwoI GCNNNNNNNGC 1 cut(s) 173
NdeII GATC 4 cut(s) 202, 220, 274, 310
NlaIII CATG 1 cut(s) 28
NlaIV GGNNCC 2 cut(s) 197, 204
NspI RCATGY 1 cut(s) 28
PceI AGGCCT 1 cut(s) 18
PdmI GAANNNNTTC 1 cut(s) 255
PfeI GAWTC 1 cut(s) 209
PkrI GCNGC 1 cut(s) 302
Psp6I CCWGG 1 cut(s) 198
PspGI CCWGG 1 cut(s) 198
PspN4I GGNNCC 2 cut(s) 197, 204
PstI CTGCAG 1 cut(s) 131
PsuI RGATCY 3 cut(s) 202, 220, 274
SaqAI TTAA 1 cut(s) 350
SatI GCNGC 1 cut(s) 301
Sau3AI GATC 4 cut(s) 202, 220, 274, 310
ScrFI CCNGG 1 cut(s) 200
SetI ASST 6 cut(s) 65, 87, 118, 155, 194, 230
SfcI CTRYAG 1 cut(s) 127
Sse9I AATT 1 cut(s) 251
SseBI AGGCCT 1 cut(s) 18
SsiI CCGC 1 cut(s) 80
SspMI CTAG 4 cut(s) 20, 113, 189, 327
StuI AGGCCT 1 cut(s) 18
StyD4I CCNGG 1 cut(s) 198
StyI CCWWGG 2 cut(s) 19, 318
TaaI ACNGT 2 cut(s) 11, 70
TaqI TCGA 2 cut(s) 65, 270
TasI AATT 1 cut(s) 251
TfiI GAWTC 1 cut(s) 209
Tru1I TTAA 1 cut(s) 350
Tru9I TTAA 1 cut(s) 350
TscAI CASTG 3 cut(s) 16, 75, 136
TseI GCWGC 1 cut(s) 300
TspRI CASTG 3 cut(s) 16, 75, 136
XapI RAATTY 1 cut(s) 251
XceI RCATGY 1 cut(s) 28
XmaJI CCTAGG 1 cut(s) 19
XmnI GAANNNNTTC 1 cut(s) 255
XspI CTAG 4 cut(s) 20, 113, 189, 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.