Rh7AG475800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0001117
Physical Location & Seq
Reverse (-)
66485429 .. 66485872
444 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG475800.1

Sequence Viewer

Length: 444 bp
ATGGATAACAGTGCAGGCCTATGCCTGTGGTTTCTCTTCTTTCTTTATTTGATCGGTGCGAGCCCGACGGTGGTGCGGGCGGAGCTTTCTGATTCTGTTGGGATGGACTGGCCGGGTTTTTTTTACCGTGGTGGTGGGTTGAAGTATGGTACTTCTTTCGGTGTTGCTTTTGGGGCAACACAGATTTATGGGTGGAGCTTGGATCCAGAATCTTTGAAGGATCTAAGGTTTTGTAAGAGGCGATACTGGGAATTTCTTCCTAAGATTATCAAAGGTCCTGGTGCGTTGAGTATTGCCGGCAGCGATGGTGATCCACCCCTGGGGTCTCGGGTCAGTGCTGATGACTGGGTTAACACGGGAAGAGAATGGCTGGTTCTAATGGTAGGCTACTGGTTTCAGTCGAATCTGTGGCGTTGCTATGCAGCGGCAACGTATGGCGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

147

Amino Acids

16.35

Weight (kDa)

6.19

Isoelectric Point (pI)

27.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 76, 80, 425, 438
AclWI GGATC 4 cut(s) 197, 210, 228, 305
AcoI YGGCCR 1 cut(s) 110
AcsI RAATTY 1 cut(s) 251
AfaI GTAC 1 cut(s) 151
AfiI CCNNNNNNNGG 2 cut(s) 70, 320
AgsI TTSAA 2 cut(s) 142, 217
AjnI CCWGG 2 cut(s) 277, 318
AluBI AGCT 2 cut(s) 85, 198
AluI AGCT 2 cut(s) 85, 198
Alw26I GTCTC 1 cut(s) 330
AlwI GGATC 4 cut(s) 197, 210, 228, 305
Ama87I CYCGRG 1 cut(s) 327
AoxI GGCC 2 cut(s) 16, 110
ApeKI GCWGC 2 cut(s) 300, 422
ApoI RAATTY 1 cut(s) 251
Asp700I GAANNNNTTC 1 cut(s) 255
AspS9I GGNCC 1 cut(s) 275
AsuC2I CCSGG 1 cut(s) 114
AsuHPI GGTGA 1 cut(s) 320
AvaI CYCGRG 1 cut(s) 327
AvaII GGWCC 1 cut(s) 275
BamHI GGATCC 1 cut(s) 202
BanII GRGCYC 1 cut(s) 65
BbvI GCAGC 2 cut(s) 312, 434
BccI CCATC 2 cut(s) 97, 299
BcgI CGANNNNNNTGC 2 cut(s) 55, 89
BciT130I CCWGG 2 cut(s) 279, 320
BcnI CCSGG 1 cut(s) 114
BcoDI GTCTC 1 cut(s) 330
BisI GCNGC 3 cut(s) 301, 423, 426
BlsI GCNGC 3 cut(s) 302, 424, 427
Bme1390I CCNGG 3 cut(s) 114, 279, 320
Bme18I GGWCC 1 cut(s) 275
BmeT110I CYCGRG 1 cut(s) 327
BmgT120I GGNCC 1 cut(s) 275
BmiI GGNNCC 1 cut(s) 204
BmrFI CCNGG 3 cut(s) 114, 279, 320
BmrI ACTGGG 2 cut(s) 256, 355
BmuI ACTGGG 2 cut(s) 256, 355
BpuMI CCSGG 1 cut(s) 114
BsaBI GATNNNNATC 1 cut(s) 309
BsaI GGTCTC 1 cut(s) 330
BsaJI CCNNGG 3 cut(s) 127, 318, 319
Bsc4I CCNNNNNNNGG 2 cut(s) 70, 320
Bse118I RCCGGY 1 cut(s) 296
Bse1I ACTGG 4 cut(s) 113, 251, 350, 395
Bse8I GATNNNNATC 1 cut(s) 309
BseBI CCWGG 2 cut(s) 279, 320
BseDI CCNNGG 3 cut(s) 127, 318, 319
BseGI GGATG 1 cut(s) 108
BseJI GATNNNNATC 1 cut(s) 309
BseLI CCNNNNNNNGG 2 cut(s) 70, 320
BseNI ACTGG 4 cut(s) 113, 251, 350, 395
BseXI GCAGC 2 cut(s) 312, 434
BsgI GTGCAG 1 cut(s) 33
BshFI GGCC 2 cut(s) 18, 112
BsiHKCI CYCGRG 1 cut(s) 327
BsiSI CCGG 2 cut(s) 113, 297
BslI CCNNNNNNNGG 2 cut(s) 70, 320
BsmAI GTCTC 1 cut(s) 330
BsnI GGCC 2 cut(s) 18, 112
Bso31I GGTCTC 1 cut(s) 330
BsoBI CYCGRG 1 cut(s) 327
Bsp1286I GDGCHC 1 cut(s) 65
Bsp143I GATC 4 cut(s) 51, 202, 220, 310
BspACI CCGC 4 cut(s) 76, 80, 425, 438
BspANI GGCC 2 cut(s) 18, 112
BspLI GGNNCC 1 cut(s) 204
BspPI GGATC 4 cut(s) 197, 210, 228, 305
BspTNI GGTCTC 1 cut(s) 330
BsrFI RCCGGY 1 cut(s) 296
BsrI ACTGG 4 cut(s) 113, 251, 350, 395
BssAI RCCGGY 1 cut(s) 296
BssECI CCNNGG 3 cut(s) 127, 318, 319
BssMI GATC 4 cut(s) 51, 202, 220, 310
Bst2UI CCWGG 2 cut(s) 279, 320
Bst4CI ACNGT 3 cut(s) 11, 70, 128
Bst6I CTCTTC 2 cut(s) 41, 355
BstC8I GCNNGC 4 cut(s) 16, 61, 78, 298
BstDEI CTNAG 2 cut(s) 224, 261
BstDSI CCRYGG 1 cut(s) 127
BstF5I GGATG 1 cut(s) 108
BstKTI GATC 4 cut(s) 54, 205, 223, 313
BstMAI GTCTC 1 cut(s) 330
BstMBI GATC 4 cut(s) 51, 202, 220, 310
BstMWI GCNNNNNNNGC 2 cut(s) 82, 173
BstNI CCWGG 2 cut(s) 279, 320
BstSCI CCNGG 3 cut(s) 112, 277, 318
BstV1I GCAGC 2 cut(s) 312, 434
BstX2I RGATCY 2 cut(s) 202, 220
BstYI RGATCY 2 cut(s) 202, 220
BsuRI GGCC 2 cut(s) 18, 112
BtgI CCRYGG 1 cut(s) 127
BtgZI GCGATG 1 cut(s) 318
BtsCI GGATG 1 cut(s) 108
BtsIMutI CAGTG 2 cut(s) 16, 340
Cac8I GCNNGC 4 cut(s) 16, 61, 78, 298
Cfr10I RCCGGY 1 cut(s) 296
Cfr13I GGNCC 1 cut(s) 275
Csp6I GTAC 1 cut(s) 150
CviJI RGCY 7 cut(s) 18, 63, 85, 112, 198, 370, 387
CviKI_1 RGCY 7 cut(s) 18, 63, 85, 112, 198, 370, 387
CviQI GTAC 1 cut(s) 150
DdeI CTNAG 2 cut(s) 224, 261
DpnI GATC 4 cut(s) 53, 204, 222, 312
DpnII GATC 4 cut(s) 51, 202, 220, 310
EaeI YGGCCR 1 cut(s) 110
Eam1104I CTCTTC 2 cut(s) 41, 355
EarI CTCTTC 2 cut(s) 41, 355
EciI GGCGGA 1 cut(s) 95
Eco147I AGGCCT 1 cut(s) 18
Eco24I GRGCYC 1 cut(s) 65
Eco31I GGTCTC 1 cut(s) 330
Eco47I GGWCC 1 cut(s) 275
Eco88I CYCGRG 1 cut(s) 327
EcoO109I RGGNCCY 1 cut(s) 275
EcoRII CCWGG 2 cut(s) 277, 318
EcoT38I GRGCYC 1 cut(s) 65
FaiI YATR 5 cut(s) 22, 147, 189, 420, 435
FauI CCCGC 1 cut(s) 69
Fnu4HI GCNGC 3 cut(s) 301, 423, 426
FokI GGATG 1 cut(s) 115
FriOI GRGCYC 1 cut(s) 65
Fsp4HI GCNGC 3 cut(s) 301, 423, 426
GluI GCNGC 3 cut(s) 301, 423, 426
HaeIII GGCC 2 cut(s) 18, 112
HapII CCGG 2 cut(s) 113, 297
HincII GTYRAC 1 cut(s) 352
HindII GTYRAC 1 cut(s) 352
HinfI GANTC 3 cut(s) 92, 209, 403
HpaI GTTAAC 1 cut(s) 352
HpaII CCGG 2 cut(s) 113, 297
HphI GGTGA 1 cut(s) 320
Hpy166II GTNNAC 1 cut(s) 352
Hpy188I TCNGA 1 cut(s) 91
Hpy188III TCNNGA 1 cut(s) 206
Hpy8I GTNNAC 1 cut(s) 352
Hpy99I CGWCG 1 cut(s) 70
HpyAV CCTTC 1 cut(s) 211
HpyCH4III ACNGT 3 cut(s) 11, 70, 128
HpyCH4IV ACGT 1 cut(s) 431
HpyCH4V TGCA 2 cut(s) 14, 422
HpyF10VI GCNNNNNNNGC 2 cut(s) 82, 173
HpyF3I CTNAG 2 cut(s) 224, 261
HpySE526I ACGT 1 cut(s) 431
KroI GCCGGC 1 cut(s) 296
KroNI GCCGGC 1 cut(s) 298
KspAI GTTAAC 1 cut(s) 352
Kzo9I GATC 4 cut(s) 51, 202, 220, 310
LmnI GCTCC 2 cut(s) 82, 195
Lsp1109I GCAGC 2 cut(s) 312, 434
MaeII ACGT 1 cut(s) 431
MalI GATC 4 cut(s) 53, 204, 222, 312
MboI GATC 4 cut(s) 51, 202, 220, 310
MboII GAAGA 3 cut(s) 28, 248, 372
MflI RGATCY 2 cut(s) 202, 220
MhlI GDGCHC 1 cut(s) 65
MluCI AATT 1 cut(s) 251
MnlI CCTC 1 cut(s) 231
MroNI GCCGGC 1 cut(s) 296
MroXI GAANNNNTTC 1 cut(s) 255
MseI TTAA 1 cut(s) 351
MspA1I CMGCKG 1 cut(s) 425
MspI CCGG 2 cut(s) 113, 297
MspR9I CCNGG 3 cut(s) 114, 279, 320
MvaI CCWGG 2 cut(s) 279, 320
MwoI GCNNNNNNNGC 2 cut(s) 82, 173
NaeI GCCGGC 1 cut(s) 298
NciI CCSGG 1 cut(s) 114
NdeII GATC 4 cut(s) 51, 202, 220, 310
NgoMIV GCCGGC 1 cut(s) 296
NlaIV GGNNCC 1 cut(s) 204
PasI CCCWGGG 1 cut(s) 319
PceI AGGCCT 1 cut(s) 18
PdiI GCCGGC 1 cut(s) 298
PdmI GAANNNNTTC 1 cut(s) 255
PfeI GAWTC 3 cut(s) 92, 209, 403
PkrI GCNGC 3 cut(s) 302, 424, 427
PpuMI RGGWCCY 1 cut(s) 275
Psp5II RGGWCCY 1 cut(s) 275
Psp6I CCWGG 2 cut(s) 277, 318
PspGI CCWGG 2 cut(s) 277, 318
PspN4I GGNNCC 1 cut(s) 204
PspPI GGNCC 1 cut(s) 275
PspPPI RGGWCCY 1 cut(s) 275
PsuI RGATCY 2 cut(s) 202, 220
RsaI GTAC 1 cut(s) 151
RsaNI GTAC 1 cut(s) 150
SaqAI TTAA 1 cut(s) 351
SatI GCNGC 3 cut(s) 301, 423, 426
Sau3AI GATC 4 cut(s) 51, 202, 220, 310
Sau96I GGNCC 1 cut(s) 275
ScrFI CCNGG 3 cut(s) 114, 279, 320
SduI GDGCHC 1 cut(s) 65
SetI ASST 5 cut(s) 87, 200, 230, 277, 434
SinI GGWCC 1 cut(s) 275
Sse9I AATT 1 cut(s) 251
SseBI AGGCCT 1 cut(s) 18
SsiI CCGC 4 cut(s) 76, 80, 425, 438
StuI AGGCCT 1 cut(s) 18
StyD4I CCNGG 3 cut(s) 112, 277, 318
TaaI ACNGT 3 cut(s) 11, 70, 128
TaiI ACGT 1 cut(s) 434
TaqI TCGA 1 cut(s) 401
TasI AATT 1 cut(s) 251
TauI GCSGC 1 cut(s) 428
TfiI GAWTC 3 cut(s) 92, 209, 403
Tru1I TTAA 1 cut(s) 351
Tru9I TTAA 1 cut(s) 351
TscAI CASTG 2 cut(s) 16, 340
TseI GCWGC 2 cut(s) 300, 422
TspRI CASTG 2 cut(s) 16, 340
VpaK11BI GGWCC 1 cut(s) 275
XapI RAATTY 1 cut(s) 251
XmnI GAANNNNTTC 1 cut(s) 255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.