Rh4AG072400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0001117
Physical Location & Seq
Reverse (-)
14871136 .. 14871751
616 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG072400.1

Sequence Viewer

Length: 522 bp
ATGGATAATAGTGCAGGCCTAGGCCTGTGGTTTCTCTTCTTTCTCTATTTGATAGGTGAGAGCCCAACGGTGGAGCTGGATGAGCTTTCTGATTCTGTTGGGATAGACTGGTCGGGTTTCTTTTACCGTGGTGGTGGGTTGAGGTGTGGTACCTCTGTCGGTGTTGCTTTTGGGGCAACACAGGTTTCTGGGTGGAGCCTGGATCTGGAATCTTTGAAGGATCTAAGGTTTTGTAAGAGGCGACACTGGGAATTTCTTCCTGAGAATATCAAAGGTCCTGGTGCGTTGGGTGTTGCCGGCAGCGATGGTGATTCACCCCTAGGTTCTCGGCGGCAACGTATGGTGGAGGAGGTGGTGATCTGGACTTTTGTGGTTGCTAACTGGCTGACAAGGATGATCGCGATGGCTCTACCGTCGTTGGGCGAGGTGGTCTGCTATGTCAAAGTCCTCTGGAAGCAGCGCAGGTTGTCGGTGGTGGTGGTGGTCTGGAATTCCATGGAGAAAGCTATGGGGTGCCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

173

Amino Acids

19.19

Weight (kDa)

5.86

Isoelectric Point (pI)

47.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 453
Acc65I GGTACC 1 cut(s) 149
AccB1I GGYRCC 2 cut(s) 149, 513
AccII CGCG 1 cut(s) 401
AciI CCGC 1 cut(s) 331
AclWI GGATC 2 cut(s) 210, 228
AcsI RAATTY 2 cut(s) 251, 490
AfaI GTAC 1 cut(s) 151
AfiI CCNNNNNNNGG 3 cut(s) 70, 205, 419
AgsI TTSAA 1 cut(s) 217
AjnI CCWGG 2 cut(s) 198, 277
AluBI AGCT 3 cut(s) 76, 85, 506
AluI AGCT 3 cut(s) 76, 85, 506
AlwI GGATC 2 cut(s) 210, 228
AoxI GGCC 2 cut(s) 16, 22
ApeKI GCWGC 2 cut(s) 300, 457
ApoI RAATTY 2 cut(s) 251, 490
Asp700I GAANNNNTTC 1 cut(s) 255
Asp718I GGTACC 1 cut(s) 149
AspA2I CCTAGG 2 cut(s) 19, 319
AspLEI GCGC 1 cut(s) 462
AspS9I GGNCC 1 cut(s) 275
AsuHPI GGTGA 4 cut(s) 68, 306, 320, 367
AvaII GGWCC 1 cut(s) 275
AvrII CCTAGG 2 cut(s) 19, 319
BaeGI GKGCMC 1 cut(s) 518
BaeI ACNNNNGTAYC 2 cut(s) 141, 174
BanI GGYRCC 2 cut(s) 149, 513
BanII GRGCYC 1 cut(s) 65
BbvI GCAGC 2 cut(s) 312, 469
BccI CCATC 2 cut(s) 299, 397
BciT130I CCWGG 2 cut(s) 200, 279
BfaI CTAG 2 cut(s) 20, 320
BfuAI ACCTGC 1 cut(s) 453
BisI GCNGC 3 cut(s) 301, 332, 458
BlnI CCTAGG 2 cut(s) 19, 319
BlsI GCNGC 3 cut(s) 302, 333, 459
Bme1390I CCNGG 2 cut(s) 200, 279
Bme18I GGWCC 1 cut(s) 275
BmgT120I GGNCC 1 cut(s) 275
BmiI GGNNCC 3 cut(s) 151, 197, 515
BmrFI CCNGG 2 cut(s) 200, 279
BmrI ACTGGG 1 cut(s) 256
BmuI ACTGGG 1 cut(s) 256
BsaJI CCNNGG 4 cut(s) 19, 127, 319, 495
Bsc4I CCNNNNNNNGG 3 cut(s) 70, 205, 419
Bse118I RCCGGY 1 cut(s) 296
Bse1I ACTGG 3 cut(s) 113, 251, 386
BseBI CCWGG 2 cut(s) 200, 279
BseDI CCNNGG 4 cut(s) 19, 127, 319, 495
BseGI GGATG 2 cut(s) 85, 399
BseLI CCNNNNNNNGG 3 cut(s) 70, 205, 419
BseMII CTCAG 1 cut(s) 252
BseNI ACTGG 3 cut(s) 113, 251, 386
BseRI GAGGAG 1 cut(s) 362
BseSI GKGCMC 1 cut(s) 518
BseXI GCAGC 2 cut(s) 312, 469
BsgI GTGCAG 1 cut(s) 33
Bsh1236I CGCG 1 cut(s) 401
BshFI GGCC 2 cut(s) 18, 24
BshNI GGYRCC 2 cut(s) 149, 513
BsiSI CCGG 1 cut(s) 297
BslI CCNNNNNNNGG 3 cut(s) 70, 205, 419
BsnI GGCC 2 cut(s) 18, 24
Bsp1286I GDGCHC 2 cut(s) 65, 518
Bsp143I GATC 4 cut(s) 202, 220, 357, 396
Bsp19I CCATGG 1 cut(s) 495
Bsp68I TCGCGA 1 cut(s) 401
BspACI CCGC 1 cut(s) 331
BspANI GGCC 2 cut(s) 18, 24
BspCNI CTCAG 1 cut(s) 253
BspFNI CGCG 1 cut(s) 401
BspLI GGNNCC 3 cut(s) 151, 197, 515
BspMI ACCTGC 1 cut(s) 453
BspPI GGATC 2 cut(s) 210, 228
BspT107I GGYRCC 2 cut(s) 149, 513
BsrFI RCCGGY 1 cut(s) 296
BsrI ACTGG 3 cut(s) 113, 251, 386
BssAI RCCGGY 1 cut(s) 296
BssECI CCNNGG 4 cut(s) 19, 127, 319, 495
BssMI GATC 4 cut(s) 202, 220, 357, 396
BssT1I CCWWGG 3 cut(s) 19, 319, 495
Bst2UI CCWGG 2 cut(s) 200, 279
Bst4CI ACNGT 3 cut(s) 70, 128, 414
Bst6I CTCTTC 1 cut(s) 41
BstC8I GCNNGC 2 cut(s) 16, 298
BstDEI CTNAG 3 cut(s) 224, 261, 519
BstDSI CCRYGG 2 cut(s) 127, 495
BstF5I GGATG 2 cut(s) 85, 399
BstFNI CGCG 1 cut(s) 401
BstHHI GCGC 1 cut(s) 462
BstKTI GATC 4 cut(s) 205, 223, 360, 399
BstMBI GATC 4 cut(s) 202, 220, 357, 396
BstMWI GCNNNNNNNGC 2 cut(s) 82, 173
BstNI CCWGG 2 cut(s) 200, 279
BstSCI CCNGG 2 cut(s) 198, 277
BstSLI GKGCMC 1 cut(s) 518
BstUI CGCG 1 cut(s) 401
BstV1I GCAGC 2 cut(s) 312, 469
BstX2I RGATCY 2 cut(s) 202, 220
BstYI RGATCY 2 cut(s) 202, 220
BsuRI GGCC 2 cut(s) 18, 24
BtgI CCRYGG 2 cut(s) 127, 495
BtgZI GCGATG 2 cut(s) 318, 416
BtsCI GGATG 2 cut(s) 85, 399
BtsIMutI CAGTG 1 cut(s) 244
BtuMI TCGCGA 1 cut(s) 401
BveI ACCTGC 1 cut(s) 453
Cac8I GCNNGC 2 cut(s) 16, 298
CfoI GCGC 1 cut(s) 462
Cfr10I RCCGGY 1 cut(s) 296
Cfr13I GGNCC 1 cut(s) 275
Csp6I GTAC 1 cut(s) 150
CviAII CATG 1 cut(s) 496
CviJI RGCY 9 cut(s) 18, 24, 63, 76, 85, 198, 385, 407, 506
CviKI_1 RGCY 9 cut(s) 18, 24, 63, 76, 85, 198, 385, 407, 506
CviQI GTAC 1 cut(s) 150
DdeI CTNAG 3 cut(s) 224, 261, 519
DpnI GATC 4 cut(s) 204, 222, 359, 398
DpnII GATC 4 cut(s) 202, 220, 357, 396
Eam1104I CTCTTC 1 cut(s) 41
EarI CTCTTC 1 cut(s) 41
Eco130I CCWWGG 3 cut(s) 19, 319, 495
Eco147I AGGCCT 2 cut(s) 18, 24
Eco24I GRGCYC 1 cut(s) 65
Eco47I GGWCC 1 cut(s) 275
EcoO109I RGGNCCY 1 cut(s) 275
EcoRI GAATTC 1 cut(s) 490
EcoRII CCWGG 2 cut(s) 198, 277
EcoT14I CCWWGG 3 cut(s) 19, 319, 495
EcoT38I GRGCYC 1 cut(s) 65
ErhI CCWWGG 3 cut(s) 19, 319, 495
FaeI CATG 1 cut(s) 499
FaiI YATR 4 cut(s) 341, 438, 497, 509
FatI CATG 1 cut(s) 495
Fnu4HI GCNGC 3 cut(s) 301, 332, 458
FokI GGATG 2 cut(s) 92, 406
FriOI GRGCYC 1 cut(s) 65
Fsp4HI GCNGC 3 cut(s) 301, 332, 458
FspBI CTAG 2 cut(s) 20, 320
GlaI GCGC 1 cut(s) 461
GluI GCNGC 3 cut(s) 301, 332, 458
HaeIII GGCC 2 cut(s) 18, 24
HapII CCGG 1 cut(s) 297
HhaI GCGC 1 cut(s) 462
Hin1II CATG 1 cut(s) 499
Hin6I GCGC 1 cut(s) 460
HinP1I GCGC 1 cut(s) 460
HinfI GANTC 3 cut(s) 92, 209, 311
HpaII CCGG 1 cut(s) 297
HphI GGTGA 4 cut(s) 68, 306, 320, 367
Hpy188I TCNGA 1 cut(s) 91
Hpy188III TCNNGA 6 cut(s) 206, 260, 361, 400, 451, 487
Hpy99I CGWCG 1 cut(s) 418
HpyAV CCTTC 1 cut(s) 211
HpyCH4III ACNGT 3 cut(s) 70, 128, 414
HpyCH4IV ACGT 1 cut(s) 337
HpyCH4V TGCA 1 cut(s) 14
HpyF10VI GCNNNNNNNGC 2 cut(s) 82, 173
HpyF3I CTNAG 3 cut(s) 224, 261, 519
HpySE526I ACGT 1 cut(s) 337
Hsp92II CATG 1 cut(s) 499
HspAI GCGC 1 cut(s) 460
KpnI GGTACC 1 cut(s) 153
KroI GCCGGC 1 cut(s) 296
KroNI GCCGGC 1 cut(s) 298
Kzo9I GATC 4 cut(s) 202, 220, 357, 396
LmnI GCTCC 2 cut(s) 73, 195
Lsp1109I GCAGC 2 cut(s) 312, 469
MaeI CTAG 2 cut(s) 20, 320
MaeII ACGT 1 cut(s) 337
MalI GATC 4 cut(s) 204, 222, 359, 398
MboI GATC 4 cut(s) 202, 220, 357, 396
MboII GAAGA 2 cut(s) 28, 248
MflI RGATCY 2 cut(s) 202, 220
MhlI GDGCHC 2 cut(s) 65, 518
MluCI AATT 2 cut(s) 251, 490
MnlI CCTC 7 cut(s) 135, 163, 231, 340, 343, 418, 458
MroNI GCCGGC 1 cut(s) 296
MroXI GAANNNNTTC 1 cut(s) 255
MspI CCGG 1 cut(s) 297
MspR9I CCNGG 2 cut(s) 200, 279
MvaI CCWGG 2 cut(s) 200, 279
MvnI CGCG 1 cut(s) 401
MwoI GCNNNNNNNGC 2 cut(s) 82, 173
NaeI GCCGGC 1 cut(s) 298
NcoI CCATGG 1 cut(s) 495
NdeII GATC 4 cut(s) 202, 220, 357, 396
NgoMIV GCCGGC 1 cut(s) 296
NlaIII CATG 1 cut(s) 499
NlaIV GGNNCC 3 cut(s) 151, 197, 515
NmeAIII GCCGAG 1 cut(s) 307
NruI TCGCGA 1 cut(s) 401
PceI AGGCCT 2 cut(s) 18, 24
PcsI WCGNNNNNNNCGW 1 cut(s) 334
PdiI GCCGGC 1 cut(s) 298
PdmI GAANNNNTTC 1 cut(s) 255
PfeI GAWTC 3 cut(s) 92, 209, 311
PkrI GCNGC 3 cut(s) 302, 333, 459
PpuMI RGGWCCY 1 cut(s) 275
Psp5II RGGWCCY 1 cut(s) 275
Psp6I CCWGG 2 cut(s) 198, 277
PspGI CCWGG 2 cut(s) 198, 277
PspN4I GGNNCC 3 cut(s) 151, 197, 515
PspPI GGNCC 1 cut(s) 275
PspPPI RGGWCCY 1 cut(s) 275
PsuI RGATCY 2 cut(s) 202, 220
RruI TCGCGA 1 cut(s) 401
RsaI GTAC 1 cut(s) 151
RsaNI GTAC 1 cut(s) 150
SatI GCNGC 3 cut(s) 301, 332, 458
Sau3AI GATC 4 cut(s) 202, 220, 357, 396
Sau96I GGNCC 1 cut(s) 275
ScrFI CCNGG 2 cut(s) 200, 279
SduI GDGCHC 2 cut(s) 65, 518
SinI GGWCC 1 cut(s) 275
Sse9I AATT 2 cut(s) 251, 490
SseBI AGGCCT 2 cut(s) 18, 24
SsiI CCGC 1 cut(s) 331
SspMI CTAG 2 cut(s) 20, 320
StuI AGGCCT 2 cut(s) 18, 24
StyD4I CCNGG 2 cut(s) 198, 277
StyI CCWWGG 3 cut(s) 19, 319, 495
TaaI ACNGT 3 cut(s) 70, 128, 414
TaiI ACGT 1 cut(s) 340
TasI AATT 2 cut(s) 251, 490
TauI GCSGC 1 cut(s) 334
TfiI GAWTC 3 cut(s) 92, 209, 311
TscAI CASTG 1 cut(s) 251
TseI GCWGC 2 cut(s) 300, 457
TspRI CASTG 1 cut(s) 251
VpaK11BI GGWCC 1 cut(s) 275
XapI RAATTY 2 cut(s) 251, 490
XmaJI CCTAGG 2 cut(s) 19, 319
XmnI GAANNNNTTC 1 cut(s) 255
XspI CTAG 2 cut(s) 20, 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.