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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 74 |
| AcuI | CTGAAG | 1 cut(s) | 126 |
| ApoI | RAATTY | 1 cut(s) | 74 |
| AsuHPI | GGTGA | 1 cut(s) | 16 |
| AsuNHI | GCTAGC | 1 cut(s) | 28 |
| BanII | GRGCYC | 1 cut(s) | 25 |
| BfaI | CTAG | 1 cut(s) | 29 |
| BmiI | GGNNCC | 1 cut(s) | 45 |
| BmtI | GCTAGC | 1 cut(s) | 32 |
| BseGI | GGATG | 1 cut(s) | 18 |
| Bsp1286I | GDGCHC | 1 cut(s) | 25 |
| BspLI | GGNNCC | 1 cut(s) | 45 |
| BspOI | GCTAGC | 1 cut(s) | 32 |
| Bst6I | CTCTTC | 1 cut(s) | 63 |
| BstC8I | GCNNGC | 2 cut(s) | 30, 48 |
| BstF5I | GGATG | 1 cut(s) | 18 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 29 |
| BtsCI | GGATG | 1 cut(s) | 18 |
| Cac8I | GCNNGC | 2 cut(s) | 30, 48 |
| CviJI | RGCY | 3 cut(s) | 23, 28, 46 |
| CviKI_1 | RGCY | 3 cut(s) | 23, 28, 46 |
| Eam1104I | CTCTTC | 1 cut(s) | 63 |
| EarI | CTCTTC | 1 cut(s) | 63 |
| Eco24I | GRGCYC | 1 cut(s) | 25 |
| Eco57I | CTGAAG | 1 cut(s) | 126 |
| EcoT38I | GRGCYC | 1 cut(s) | 25 |
| FaiI | YATR | 1 cut(s) | 98 |
| FokI | GGATG | 1 cut(s) | 25 |
| FriOI | GRGCYC | 1 cut(s) | 25 |
| FspBI | CTAG | 1 cut(s) | 29 |
| HphI | GGTGA | 1 cut(s) | 16 |
| Hpy188I | TCNGA | 4 cut(s) | 63, 106, 130, 139 |
| HpyAV | CCTTC | 2 cut(s) | 32, 101 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 29 |
| LmnI | GCTCC | 1 cut(s) | 43 |
| LpnPI | CCDG | 1 cut(s) | 60 |
| MaeI | CTAG | 1 cut(s) | 29 |
| MboII | GAAGA | 1 cut(s) | 50 |
| MhlI | GDGCHC | 1 cut(s) | 25 |
| MluCI | AATT | 1 cut(s) | 74 |
| MmeI | TCCRAC | 1 cut(s) | 86 |
| MseI | TTAA | 1 cut(s) | 72 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 29 |
| NheI | GCTAGC | 1 cut(s) | 28 |
| NlaIV | GGNNCC | 1 cut(s) | 45 |
| NmeAIII | GCCGAG | 1 cut(s) | 4 |
| PspN4I | GGNNCC | 1 cut(s) | 45 |
| SaqAI | TTAA | 1 cut(s) | 72 |
| SduI | GDGCHC | 1 cut(s) | 25 |
| SetI | ASST | 1 cut(s) | 112 |
| SgeI | CNNG | 5 cut(s) | 30, 36, 41, 59, 134 |
| Sse9I | AATT | 1 cut(s) | 74 |
| SspMI | CTAG | 1 cut(s) | 29 |
| TasI | AATT | 1 cut(s) | 74 |
| Tru1I | TTAA | 1 cut(s) | 72 |
| Tru9I | TTAA | 1 cut(s) | 72 |
| XapI | RAATTY | 1 cut(s) | 74 |
| XspI | CTAG | 1 cut(s) | 29 |