FvH4_6g50540

Protein of unknown function (DUF3754)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000718
Physical Location & Seq
Reverse (-)
37591828 .. 37597409
5582 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g50540.t2

Sequence Viewer

Length: 1470 bp
ATGGAAAAGAAGAAGGACATGATTCGTATAGAGCGCGAGTCCGTGATTCCAGTCCTCAAGCCCAAGCTCATCATGACCTTGGCCAACCTCATCGAGCATAGTTCCGACCGGGCGGAGTTTCTGAAGCTGTGCAAGAGAATTGAGTACACCGTTCGGGCTTGGTATCTTTTGCAGTTCGAGGATTTGATGCAACTGTATTCGCTGTTCGACCCCGTAAATGGGAATCAGAAGTTAAAGCAGCAGAATCTTTCTCCGAAAGAAACCGATGTGCTGGAGCAGAATTTCCTCACTTACTTAATGGAGGTGATGGAGAAGAGTAATTTCAAGATTGCAAGTGATGAAGAGATTGAGGTTGCGCATTCGGGGCAGTATCTTCTGAATCTTCCCATCGTGGTTGATGAATCTAAGCTTGACAAGAAGCTTCTGAAGACCTTCTTTGAAGAGAATCCTCAGCCAAACCTTCCTGATTTTGCTGATAAGTACATTATCTTCCGTCGTGGTGTTGGACTTGATCAGACAACTGATTACTTTTTCATGGAGAAAGTGGACATGATCATTGGACGTTTTTGGGCATATCTTTTACGAATAACTAGGGTTGAAAAGCTTTTATCCAGAAGGTCAAGTAGTCGGCGTACAAAAGATCCAAAGAAGGATGATGAAATCACCTCAGAAGCCAACGGGGAGGAGGAGGAGGACTTATATGTTGAACGAATTCGTCTTGAAAATATGGAACTAAGTGTTAAAGGCTTACTGAGCAAGACGACAATCAAAGAGCCAACCTTTGATAGGATAATTGTTGTTTACAGGCGAGCAAGTCCCAAAGCAAAAGAAGAACGAGGAATATTTGTGAAACATTTCAAAAATATTCCAATGGCTGATATGGAGATAGTTCTTCCCGAGAAGAAGAACCCAGGATTAACTCCAATGGACTGGGTCAAGTTCCTTGGCTCTGCTGTAGTTGGGCTGGTAGCTGTTATTAGTTCGCTTGAAATGCCTAATGCTGACTTTTGGGTCATCATTGCAATTCTTTCCACTGTGATTGGTTACTGTGCTAAGATATACTTCAGTTTTCAGCAGAACTTGGCTGCATATCAGAACCTAATAACACAGTCCATGTATGACAAGCAGCTGGATAGTGGAAGAGGTACTCTTCTTCACTTGTGCGATGATGTGATTCAACAGGAGGTCAAGGAGGTGATACTTTCTTTCTATATATTAATGGAACAGGGAAAAGCTACAAGAGAAGATCTGGATAGGTGGTGTGAGGAACTCATTAAAGAAGAGTTTGGCGCCAGCTGTAATTTTGATGTGGATGATGCAGTTGAGAAGTTGGAAAAATTGGGAATTGTCTCTCGGGATTCCGTTGGACGGTTTTTCTGTGTTGGGCTGAAACGTGCAAATGAGATAATTGGGACAACCACTGAGGAGCTTGTTCTGAGGGCAAAACAAGGCAAACAAGGCAGTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3754

Protein Analysis

490

Amino Acids

56.54

Weight (kDa)

5.42

Isoelectric Point (pI)

38.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3754 PF12576 261 - 382 1.4e-22 Protein of unknown function (DUF3754)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1010
Acc16I TGCGCA 1 cut(s) 357
AccB1I GGYRCC 1 cut(s) 1289
AccII CGCG 1 cut(s) 36
AciI CCGC 1 cut(s) 113
AclWI GGATC 1 cut(s) 635
AcoI YGGCCR 1 cut(s) 81
AcsI RAATTY 2 cut(s) 280, 711
AcuI CTGAAG 3 cut(s) 143, 446, 1048
AcyI GRCGYC 1 cut(s) 1290
AfaI GTAC 5 cut(s) 146, 482, 634, 1147, 1465
AfiI CCNNNNNNNGG 4 cut(s) 218, 219, 786, 1368
AgsI TTSAA 8 cut(s) 325, 440, 599, 707, 722, 859, 989, 1178
AjnI CCWGG 1 cut(s) 910
Alw26I GTCTC 1 cut(s) 1354
AlwI GGATC 1 cut(s) 635
Ama87I CYCGRG 2 cut(s) 896, 1353
AoxI GGCC 1 cut(s) 81
ApeKI GCWGC 3 cut(s) 238, 1085, 1126
ApoI RAATTY 2 cut(s) 280, 711
AseI ATTAAT 1 cut(s) 1217
Asp700I GAANNNNTTC 3 cut(s) 431, 711, 854
AspLEI GCGC 3 cut(s) 36, 358, 1292
AsuC2I CCSGG 1 cut(s) 110
AsuHPI GGTGA 3 cut(s) 316, 655, 1207
AvaI CYCGRG 2 cut(s) 896, 1353
BalI TGGCCA 1 cut(s) 83
BanI GGYRCC 1 cut(s) 1289
BarI GAAGNNNNNNTAC 2 cut(s) 473, 505
BbsI GAAGAC 1 cut(s) 434
BbvCI CCTCAGC 1 cut(s) 450
BbvI GCAGC 3 cut(s) 250, 1072, 1138
BccI CCATC 2 cut(s) 301, 395
BciT130I CCWGG 1 cut(s) 912
BclI TGATCA 2 cut(s) 511, 552
BcnI CCSGG 1 cut(s) 110
BcoDI GTCTC 1 cut(s) 1354
BfaI CTAG 1 cut(s) 591
BfmI CTRYAG 1 cut(s) 954
BfoI RGCGCY 1 cut(s) 1293
BglII AGATCT 1 cut(s) 1246
BisI GCNGC 3 cut(s) 239, 1086, 1127
BlsI GCNGC 3 cut(s) 240, 1087, 1128
BmcAI AGTACT 1 cut(s) 1465
Bme1390I CCNGG 2 cut(s) 110, 912
BmeT110I CYCGRG 2 cut(s) 896, 1353
BmiI GGNNCC 1 cut(s) 1291
BmrFI CCNGG 2 cut(s) 110, 912
BmrI ACTGGG 1 cut(s) 940
BmsI GCATC 2 cut(s) 177, 1306
BmuI ACTGGG 1 cut(s) 940
BpiI GAAGAC 1 cut(s) 434
BpmI CTGGAG 1 cut(s) 293
Bpu10I CCTNAGC 1 cut(s) 450
BpuEI CTTGAG 1 cut(s) 41
BpuMI CCSGG 1 cut(s) 110
BsaHI GRCGYC 1 cut(s) 1290
BsaJI CCNNGG 3 cut(s) 78, 910, 943
Bsc4I CCNNNNNNNGG 4 cut(s) 218, 219, 786, 1368
Bse1I ACTGG 2 cut(s) 50, 935
Bse3DI GCAATG 1 cut(s) 1017
BseBI CCWGG 1 cut(s) 912
BseDI CCNNGG 3 cut(s) 78, 910, 943
BseGI GGATG 2 cut(s) 658, 1318
BseLI CCNNNNNNNGG 4 cut(s) 218, 219, 786, 1368
BseMI GCAATG 1 cut(s) 1017
BseMII CTCAG 5 cut(s) 464, 681, 743, 1413, 1427
BseNI ACTGG 2 cut(s) 50, 935
BseRI GAGGAG 4 cut(s) 698, 701, 704, 1439
BseXI GCAGC 3 cut(s) 250, 1072, 1138
Bsh1236I CGCG 1 cut(s) 36
Bsh1285I CGRYCG 1 cut(s) 109
BshFI GGCC 1 cut(s) 83
BshNI GGYRCC 1 cut(s) 1289
BsiEI CGRYCG 1 cut(s) 109
BsiHKCI CYCGRG 2 cut(s) 896, 1353
BsiSI CCGG 1 cut(s) 109
BslFI GGGAC 2 cut(s) 801, 1426
BslI CCNNNNNNNGG 4 cut(s) 218, 219, 786, 1368
BsmAI GTCTC 1 cut(s) 1354
BsmFI GGGAC 2 cut(s) 801, 1426
BsmI GAATGC 1 cut(s) 358
BsnI GGCC 1 cut(s) 83
BsoBI CYCGRG 2 cut(s) 896, 1353
Bsp143I GATC 4 cut(s) 511, 552, 640, 1246
BspACI CCGC 1 cut(s) 113
BspANI GGCC 1 cut(s) 83
BspCNI CTCAG 5 cut(s) 463, 680, 744, 1414, 1428
BspFNI CGCG 1 cut(s) 36
BspHI TCATGA 1 cut(s) 72
BspLI GGNNCC 1 cut(s) 1291
BspPI GGATC 1 cut(s) 635
BspT107I GGYRCC 1 cut(s) 1289
BsrDI GCAATG 1 cut(s) 1017
BsrI ACTGG 2 cut(s) 50, 935
BssECI CCNNGG 3 cut(s) 78, 910, 943
BssMI GATC 4 cut(s) 511, 552, 640, 1246
BssNI GRCGYC 1 cut(s) 1290
BssT1I CCWWGG 2 cut(s) 78, 943
Bst2UI CCWGG 1 cut(s) 912
Bst4CI ACNGT 6 cut(s) 151, 195, 1036, 1049, 1110, 1371
Bst6I CTCTTC 6 cut(s) 308, 336, 435, 1135, 1155, 1275
BstACI GRCGYC 1 cut(s) 1290
BstC8I GCNNGC 2 cut(s) 810, 1294
BstDEI CTNAG 8 cut(s) 405, 450, 667, 734, 752, 1053, 1422, 1436
BstENI CCTNNNNNAGG 1 cut(s) 784
BstF5I GGATG 2 cut(s) 658, 1318
BstFNI CGCG 1 cut(s) 36
BstH2I RGCGCY 1 cut(s) 1293
BstHHI GCGC 3 cut(s) 36, 358, 1292
BstKTI GATC 4 cut(s) 514, 555, 643, 1249
BstMAI GTCTC 1 cut(s) 1354
BstMBI GATC 4 cut(s) 511, 552, 640, 1246
BstMCI CGRYCG 1 cut(s) 109
BstMWI GCNNNNNNNGC 4 cut(s) 364, 753, 991, 1458
BstNI CCWGG 1 cut(s) 912
BstSCI CCNGG 2 cut(s) 108, 910
BstSFI CTRYAG 1 cut(s) 954
BstUI CGCG 1 cut(s) 36
BstV1I GCAGC 3 cut(s) 250, 1072, 1138
BstV2I GAAGAC 1 cut(s) 434
BstX2I RGATCY 2 cut(s) 640, 1246
BstXI CCANNNNNNTGG 1 cut(s) 930
BstYI RGATCY 2 cut(s) 640, 1246
BsuRI GGCC 1 cut(s) 83
BtgZI GCGATG 1 cut(s) 1179
BtsCI GGATG 2 cut(s) 658, 1318
BtsIMutI CAGTG 2 cut(s) 1032, 1419
Cac8I GCNNGC 2 cut(s) 810, 1294
CciI TCATGA 1 cut(s) 72
CfoI GCGC 3 cut(s) 36, 358, 1292
Csp6I GTAC 5 cut(s) 145, 481, 633, 1146, 1464
CspCI CAANNNNNGTGG 2 cut(s) 1021, 1056
CviAII CATG 5 cut(s) 19, 73, 535, 550, 1114
CviQI GTAC 5 cut(s) 145, 481, 633, 1146, 1464
DdeI CTNAG 8 cut(s) 405, 450, 667, 734, 752, 1053, 1422, 1436
DinI GGCGCC 1 cut(s) 1291
DpnI GATC 4 cut(s) 513, 554, 642, 1248
DpnII GATC 4 cut(s) 511, 552, 640, 1246
DrdI GACNNNNNNGTC 1 cut(s) 1010
DseDI GACNNNNNNGTC 1 cut(s) 1010
EaeI YGGCCR 1 cut(s) 81
Eam1104I CTCTTC 6 cut(s) 308, 336, 435, 1135, 1155, 1275
EarI CTCTTC 6 cut(s) 308, 336, 435, 1135, 1155, 1275
EciI GGCGGA 1 cut(s) 128
Eco130I CCWWGG 2 cut(s) 78, 943
Eco57I CTGAAG 3 cut(s) 143, 446, 1048
Eco88I CYCGRG 2 cut(s) 896, 1353
EcoNI CCTNNNNNAGG 1 cut(s) 784
EcoRI GAATTC 1 cut(s) 711
EcoRII CCWGG 1 cut(s) 910
EcoT14I CCWWGG 2 cut(s) 78, 943
EgeI GGCGCC 1 cut(s) 1291
EheI GGCGCC 1 cut(s) 1291
ErhI CCWWGG 2 cut(s) 78, 943
FaeI CATG 5 cut(s) 22, 76, 538, 553, 1117
FalI AAGNNNNNCTT 4 cut(s) 419, 451, 1046, 1078
FaqI GGGAC 2 cut(s) 801, 1426
FatI CATG 5 cut(s) 18, 72, 534, 549, 1113
FbaI TGATCA 2 cut(s) 511, 552
Fnu4HI GCNGC 3 cut(s) 239, 1086, 1127
FokI GGATG 2 cut(s) 665, 1325
Fsp4HI GCNGC 3 cut(s) 239, 1086, 1127
FspBI CTAG 1 cut(s) 591
FspI TGCGCA 1 cut(s) 357
GlaI GCGC 3 cut(s) 35, 357, 1291
GluI GCNGC 3 cut(s) 239, 1086, 1127
GsuI CTGGAG 1 cut(s) 293
HaeII RGCGCY 1 cut(s) 1293
HaeIII GGCC 1 cut(s) 83
HapII CCGG 1 cut(s) 109
HhaI GCGC 3 cut(s) 36, 358, 1292
Hin1I GRCGYC 1 cut(s) 1290
Hin1II CATG 5 cut(s) 22, 76, 538, 553, 1117
Hin6I GCGC 3 cut(s) 34, 356, 1290
HinP1I GCGC 3 cut(s) 34, 356, 1290
HindIII AAGCTT 3 cut(s) 407, 419, 602
HpaII CCGG 1 cut(s) 109
HphI GGTGA 3 cut(s) 316, 655, 1207
Hpy166II GTNNAC 3 cut(s) 147, 547, 802
Hpy188III TCNNGA 8 cut(s) 73, 325, 464, 612, 719, 896, 1250, 1355
Hpy8I GTNNAC 3 cut(s) 147, 547, 802
Hpy99I CGWCG 1 cut(s) 498
HpyAV CCTTC 5 cut(s) 7, 442, 470, 609, 643
HpyCH4III ACNGT 6 cut(s) 151, 195, 1036, 1049, 1110, 1371
HpyCH4IV ACGT 2 cut(s) 562, 1393
HpyCH4V TGCA 8 cut(s) 132, 172, 190, 332, 1022, 1088, 1319, 1397
HpyF10VI GCNNNNNNNGC 4 cut(s) 364, 753, 991, 1458
HpyF3I CTNAG 8 cut(s) 405, 450, 667, 734, 752, 1053, 1422, 1436
HpySE526I ACGT 2 cut(s) 562, 1393
Hsp92I GRCGYC 1 cut(s) 1290
Hsp92II CATG 5 cut(s) 22, 76, 538, 553, 1117
HspAI GCGC 3 cut(s) 34, 356, 1290
KasI GGCGCC 1 cut(s) 1289
Ksp22I TGATCA 2 cut(s) 511, 552
Kzo9I GATC 4 cut(s) 511, 552, 640, 1246
LmnI GCTCC 2 cut(s) 274, 1426
Lsp1109I GCAGC 3 cut(s) 250, 1072, 1138
LweI GCATC 2 cut(s) 177, 1306
MaeI CTAG 1 cut(s) 591
MaeII ACGT 2 cut(s) 562, 1393
MaeIII GTNAC 1 cut(s) 1043
MalI GATC 4 cut(s) 513, 554, 642, 1248
MboI GATC 4 cut(s) 511, 552, 640, 1246
MflI RGATCY 2 cut(s) 640, 1246
MlsI TGGCCA 1 cut(s) 83
MluNI TGGCCA 1 cut(s) 83
Mly113I GGCGCC 1 cut(s) 1290
MlyI GAGTC 1 cut(s) 47
MmeI TCCRAC 4 cut(s) 129, 484, 1311, 1345
Mox20I TGGCCA 1 cut(s) 83
MroXI GAANNNNTTC 3 cut(s) 431, 711, 854
MscI TGGCCA 1 cut(s) 83
MseI TTAA 6 cut(s) 233, 296, 741, 917, 1217, 1275
Msp20I TGGCCA 1 cut(s) 83
MspA1I CMGCKG 2 cut(s) 1129, 1296
MspI CCGG 1 cut(s) 109
MspR9I CCNGG 2 cut(s) 110, 912
Mva1269I GAATGC 1 cut(s) 358
MvaI CCWGG 1 cut(s) 912
MvnI CGCG 1 cut(s) 36
MwoI GCNNNNNNNGC 4 cut(s) 364, 753, 991, 1458
NarI GGCGCC 1 cut(s) 1290
NciI CCSGG 1 cut(s) 110
NdeII GATC 4 cut(s) 511, 552, 640, 1246
NlaIII CATG 5 cut(s) 22, 76, 538, 553, 1117
NlaIV GGNNCC 1 cut(s) 1291
NsbI TGCGCA 1 cut(s) 357
PagI TCATGA 1 cut(s) 72
PctI GAATGC 1 cut(s) 358
PdmI GAANNNNTTC 3 cut(s) 431, 711, 854
PfeI GAWTC 9 cut(s) 22, 46, 223, 244, 379, 401, 445, 1174, 1358
PflFI GACNNNGTC 1 cut(s) 932
PkrI GCNGC 3 cut(s) 240, 1087, 1128
PleI GAGTC 1 cut(s) 46
PluTI GGCGCC 1 cut(s) 1293
PpsI GAGTC 1 cut(s) 46
PshBI ATTAAT 1 cut(s) 1217
Psp6I CCWGG 1 cut(s) 910
PspGI CCWGG 1 cut(s) 910
PspN4I GGNNCC 1 cut(s) 1291
PsrI GAACNNNNNNTAC 2 cut(s) 188, 220
PsuI RGATCY 2 cut(s) 640, 1246
PsyI GACNNNGTC 1 cut(s) 932
PvuII CAGCTG 2 cut(s) 1129, 1296
RsaI GTAC 5 cut(s) 146, 482, 634, 1147, 1465
RsaNI GTAC 5 cut(s) 145, 481, 633, 1146, 1464
SaqAI TTAA 6 cut(s) 233, 296, 741, 917, 1217, 1275
SatI GCNGC 3 cut(s) 239, 1086, 1127
Sau3AI GATC 4 cut(s) 511, 552, 640, 1246
ScaI AGTACT 1 cut(s) 1465
SchI GAGTC 1 cut(s) 47
ScrFI CCNGG 2 cut(s) 110, 912
SfaNI GCATC 2 cut(s) 177, 1306
SfcI CTRYAG 1 cut(s) 954
SfoI GGCGCC 1 cut(s) 1291
SmlI CTYRAG 1 cut(s) 56
SmoI CTYRAG 1 cut(s) 56
SsiI CCGC 1 cut(s) 113
SspDI GGCGCC 1 cut(s) 1289
SspI AATATT 2 cut(s) 843, 865
SspMI CTAG 1 cut(s) 591
StyD4I CCNGG 2 cut(s) 108, 910
StyI CCWWGG 2 cut(s) 78, 943
TaaI ACNGT 6 cut(s) 151, 195, 1036, 1049, 1110, 1371
TaiI ACGT 2 cut(s) 565, 1396
TaqI TCGA 3 cut(s) 93, 177, 207
TatI WGTACW 3 cut(s) 144, 480, 1463
TfiI GAWTC 9 cut(s) 22, 46, 223, 244, 379, 401, 445, 1174, 1358
Tru1I TTAA 6 cut(s) 233, 296, 741, 917, 1217, 1275
Tru9I TTAA 6 cut(s) 233, 296, 741, 917, 1217, 1275
TscAI CASTG 2 cut(s) 1039, 1426
TseI GCWGC 3 cut(s) 238, 1085, 1126
TspDTI ATGAA 4 cut(s) 354, 414, 523, 672
TspGWI ACGGA 3 cut(s) 31, 482, 1351
TspRI CASTG 2 cut(s) 1039, 1426
Tth111I GACNNNGTC 1 cut(s) 932
VspI ATTAAT 1 cut(s) 1217
XagI CCTNNNNNAGG 1 cut(s) 784
XapI RAATTY 2 cut(s) 280, 711
XmnI GAANNNNTTC 3 cut(s) 431, 711, 854
XspI CTAG 1 cut(s) 591
ZrmI AGTACT 1 cut(s) 1465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.