Rh2BG636700

Protein of unknown function (DUF3754)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000718
Physical Location & Seq
Reverse (-)
85743191 .. 85748948
5758 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG636700.1

Sequence Viewer

Length: 1419 bp
ATGTGTGGTCTTGCTTGGGTCTCACCTCTTCTCCCCCCGTTTTGCAAGAAACTGACACACCAAAACAGTAAAAGATACCAAACCCTCTCCCTCCCTTTACTGTCAAGAATAAACCGTTCCTCTCCTCTCTCTCTCACATTTAAACACCCTCAATCCGTTTCCATTGGCGTTCAAGAACCCGGAAATTCGTTTCTGCAGAGACCCAGAATGGAGAAAACCGCGTCCCAGAAGAAGAAAGACATGATTCGAATAGAGCGGGAGTCAGTGATTCCGGTTCTCAAGCCCAAGCTCATCATGTCCTTGGCCAACCTCATTGAACATAGTTCCGACCGGGCGGAGTTTTTAAAGCTCAGCAAGAGAATTGAGTACACGATTCGGGCTTGGTATCTTCTACAATTCGAGGATTTGATGCAACTGTATTCGCTCTTTGACCCCGTACATGGGAATCAAAAATTGGAGCAACAGAATCTTTCTCCTAAAGAGATTGATGTACTGGAACAGAATTTCCTCACGTACTTACTTCAGGTGATGGAAAAGAGTAATTTCAAGATTGCAACTGATGAAGAAATTGAGATTGCACATTCAGGGCAGTATCTTCTAAATCTTCCCATCGTGGTTGATGAATCTAAGATTGACAAGAAGCTTCTGAAGAACTTCTTTGCAGAGAATCCTCAGCCAAACCTTCCAGATTTTGCTGATAAGTACATTATCTTCCGGCGTGGTGTTGGACTTGATCAGACAACTGATTACTTTTTCATGGAGAAAGTGGACATGATCATTGCACGTTTTTGGGCATATCTTTTACGAATAACTAGGGCTGAAAAACTTTTATCCAGAAGGTCAAGTAGTCGGCACACAAAAGATCCAAAGAAGGATGATGAAATTACCTCAGAAGCAGATGGGGAGGAGGCGGAGGACTTATACGTTGAACGAATTCGTCTTGAAAATATGGAACTAAGCGTTAAAAGCTTGCTGAGCAAGACCACAATCAAAGAGCCAACCTTTGATAGGATAATTGTTGTTTACAGGCGAGCAAGTACCAAAGCAAAAGAAGAACGAGGAATATTTGTGAAACATTTCAAAAATATTCCCATGGCTGATATGGAGATAGTTCTTCCCGAGAAGAAGAACCCAGGATTAACTCCAATGGACTGGGTCAAGTTCCTTGGCTCTGCTGTAGTTGGGCTGGTGAGTTTCTTAATAGCAATGAAGTTTGTCCACGTGCACTGTTCTTGCCATTTATTAGTTTGCGAAGTGCAGCTTATTATTTCTTTCTTCTACAGGTTGCTGTTATTAGTTCGCTTGAAATGCCTAATGCTGACTTTTTGGTCATCATTGCGGTTCTTTCCACTGTCATTGGTTACTGTGCTAAGATATACTTCAGTTTTCAGCAGAACTTGGCTGCATATCAGAACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3754

Protein Analysis

472

Amino Acids

55.04

Weight (kDa)

9.32

Isoelectric Point (pI)

39.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3754 PF12576 335 - 410 8e-12 Protein of unknown function (DUF3754)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1327
AccBSI CCGCTC 1 cut(s) 256
AccII CGCG 1 cut(s) 221
AciI CCGC 5 cut(s) 219, 256, 335, 911, 1339
AclWI GGATC 1 cut(s) 857
AcoI YGGCCR 1 cut(s) 303
AcsI RAATTY 3 cut(s) 184, 502, 933
AcuI CTGAAG 3 cut(s) 506, 668, 1365
AcvI CACGTG 1 cut(s) 1222
AfaI GTAC 6 cut(s) 368, 438, 492, 515, 704, 1039
AfiI CCNNNNNNNGG 3 cut(s) 440, 441, 1008
AgsI TTSAA 7 cut(s) 173, 317, 547, 929, 944, 1081, 1306
AjnI CCWGG 1 cut(s) 1132
AjuI GAANNNNNNNTTGG 2 cut(s) 437, 469
AloI GAACNNNNNNTCC 2 cut(s) 489, 521
AluBI AGCT 5 cut(s) 289, 349, 643, 969, 1261
AluI AGCT 5 cut(s) 289, 349, 643, 969, 1261
Alw21I GWGCWC 1 cut(s) 1227
Alw26I GTCTC 2 cut(s) 25, 193
Alw44I GTGCAC 1 cut(s) 1223
AlwI GGATC 1 cut(s) 857
Ama87I CYCGRG 1 cut(s) 1118
AoxI GGCC 1 cut(s) 303
ApaLI GTGCAC 1 cut(s) 1223
ApeKI GCWGC 2 cut(s) 1258, 1402
ApoI RAATTY 3 cut(s) 184, 502, 933
Asp700I GAANNNNTTC 3 cut(s) 653, 933, 1076
AsuC2I CCSGG 2 cut(s) 180, 332
AsuHPI GGTGA 3 cut(s) 15, 538, 1201
AsuII TTCGAA 1 cut(s) 247
AvaI CYCGRG 1 cut(s) 1118
BaeGI GKGCMC 1 cut(s) 1227
BalI TGGCCA 1 cut(s) 305
BarI GAAGNNNNNNTAC 2 cut(s) 695, 727
BbrPI CACGTG 1 cut(s) 1222
Bbv12I GWGCWC 1 cut(s) 1227
BbvCI CCTCAGC 1 cut(s) 672
BbvI GCAGC 2 cut(s) 1270, 1389
BccI CCATC 3 cut(s) 523, 617, 893
BciT130I CCWGG 1 cut(s) 1134
BclI TGATCA 2 cut(s) 733, 774
BcnI CCSGG 2 cut(s) 180, 332
BcoDI GTCTC 2 cut(s) 25, 193
BfaI CTAG 1 cut(s) 813
BfmI CTRYAG 3 cut(s) 194, 1176, 1279
BisI GCNGC 2 cut(s) 1259, 1403
BlpI GCTNAGC 2 cut(s) 350, 974
BlsI GCNGC 2 cut(s) 1260, 1404
Bme1390I CCNGG 3 cut(s) 180, 332, 1134
BmeT110I CYCGRG 1 cut(s) 1118
BmrFI CCNGG 3 cut(s) 180, 332, 1134
BmrI ACTGGG 1 cut(s) 1162
BmsI GCATC 1 cut(s) 399
BmuI ACTGGG 1 cut(s) 1162
Bpu10I CCTNAGC 1 cut(s) 672
Bpu1102I GCTNAGC 2 cut(s) 350, 974
Bpu14I TTCGAA 1 cut(s) 247
BpuEI CTTGAG 1 cut(s) 263
BpuMI CCSGG 2 cut(s) 180, 332
BsaAI YACGTR 2 cut(s) 513, 1222
BsaI GGTCTC 2 cut(s) 25, 193
BsaJI CCNNGG 4 cut(s) 300, 1092, 1132, 1165
BsaWI WCCGGW 1 cut(s) 271
BsaXI ACNNNNNCTCC 2 cut(s) 15, 45
Bsc4I CCNNNNNNNGG 3 cut(s) 440, 441, 1008
Bse1I ACTGG 2 cut(s) 498, 1157
Bse3DI GCAATG 3 cut(s) 777, 1212, 1334
BseBI CCWGG 1 cut(s) 1134
BseDI CCNNGG 4 cut(s) 300, 1092, 1132, 1165
BseGI GGATG 1 cut(s) 880
BseLI CCNNNNNNNGG 3 cut(s) 440, 441, 1008
BseMI GCAATG 3 cut(s) 777, 1212, 1334
BseMII CTCAG 4 cut(s) 364, 686, 903, 965
BseNI ACTGG 2 cut(s) 498, 1157
BseRI GAGGAG 2 cut(s) 114, 920
BseSI GKGCMC 1 cut(s) 1227
BseXI GCAGC 2 cut(s) 1270, 1389
BsgI GTGCAG 1 cut(s) 1277
Bsh1236I CGCG 1 cut(s) 221
Bsh1285I CGRYCG 1 cut(s) 331
BshFI GGCC 1 cut(s) 305
BsiEI CGRYCG 1 cut(s) 331
BsiHKAI GWGCWC 1 cut(s) 1227
BsiHKCI CYCGRG 1 cut(s) 1118
BsiSI CCGG 4 cut(s) 180, 272, 331, 715
BslFI GGGAC 1 cut(s) 208
BslI CCNNNNNNNGG 3 cut(s) 440, 441, 1008
BsmAI GTCTC 2 cut(s) 25, 193
BsmFI GGGAC 1 cut(s) 208
BsnI GGCC 1 cut(s) 305
Bso31I GGTCTC 2 cut(s) 25, 193
BsoBI CYCGRG 1 cut(s) 1118
Bsp119I TTCGAA 1 cut(s) 247
Bsp1286I GDGCHC 1 cut(s) 1227
Bsp143I GATC 3 cut(s) 733, 774, 862
Bsp1720I GCTNAGC 2 cut(s) 350, 974
Bsp19I CCATGG 1 cut(s) 1092
BspACI CCGC 5 cut(s) 219, 256, 335, 911, 1339
BspANI GGCC 1 cut(s) 305
BspCNI CTCAG 4 cut(s) 363, 685, 902, 966
BspFNI CGCG 1 cut(s) 221
BspMAI CTGCAG 1 cut(s) 198
BspPI GGATC 1 cut(s) 857
BspT104I TTCGAA 1 cut(s) 247
BspTNI GGTCTC 2 cut(s) 25, 193
BsrBI CCGCTC 1 cut(s) 256
BsrDI GCAATG 3 cut(s) 777, 1212, 1334
BsrI ACTGG 2 cut(s) 498, 1157
BssECI CCNNGG 4 cut(s) 300, 1092, 1132, 1165
BssMI GATC 3 cut(s) 733, 774, 862
BssT1I CCWWGG 3 cut(s) 300, 1092, 1165
Bst2UI CCWGG 1 cut(s) 1134
Bst4CI ACNGT 7 cut(s) 68, 102, 116, 417, 1229, 1353, 1366
Bst6I CTCTTC 1 cut(s) 33
BstBAI YACGTR 2 cut(s) 513, 1222
BstBI TTCGAA 1 cut(s) 247
BstC8I GCNNGC 2 cut(s) 971, 1032
BstDEI CTNAG 7 cut(s) 350, 627, 672, 889, 956, 974, 1370
BstDSI CCRYGG 1 cut(s) 1092
BstENI CCTNNNNNAGG 1 cut(s) 1006
BstF5I GGATG 1 cut(s) 880
BstFNI CGCG 1 cut(s) 221
BstKTI GATC 3 cut(s) 736, 777, 865
BstMAI GTCTC 2 cut(s) 25, 193
BstMBI GATC 3 cut(s) 733, 774, 862
BstMCI CGRYCG 1 cut(s) 331
BstMWI GCNNNNNNNGC 3 cut(s) 966, 975, 1308
BstNI CCWGG 1 cut(s) 1134
BstSCI CCNGG 3 cut(s) 178, 330, 1132
BstSFI CTRYAG 3 cut(s) 194, 1176, 1279
BstSLI GKGCMC 1 cut(s) 1227
BstUI CGCG 1 cut(s) 221
BstV1I GCAGC 2 cut(s) 1270, 1389
BstX2I RGATCY 1 cut(s) 862
BstXI CCANNNNNNTGG 1 cut(s) 1152
BstYI RGATCY 1 cut(s) 862
BsuRI GGCC 1 cut(s) 305
BtgI CCRYGG 1 cut(s) 1092
BtsCI GGATG 1 cut(s) 880
BtsIMutI CAGTG 3 cut(s) 270, 1225, 1349
Cac8I GCNNGC 2 cut(s) 971, 1032
CseI GACGC 1 cut(s) 210
Csp6I GTAC 6 cut(s) 367, 437, 491, 514, 703, 1038
CspCI CAANNNNNGTGG 2 cut(s) 1338, 1373
CviAII CATG 6 cut(s) 241, 295, 440, 757, 772, 1093
CviQI GTAC 6 cut(s) 367, 437, 491, 514, 703, 1038
DdeI CTNAG 7 cut(s) 350, 627, 672, 889, 956, 974, 1370
DpnI GATC 3 cut(s) 735, 776, 864
DpnII GATC 3 cut(s) 733, 774, 862
DraI TTTAAA 2 cut(s) 142, 345
DrdI GACNNNNNNGTC 1 cut(s) 1327
DseDI GACNNNNNNGTC 1 cut(s) 1327
EaeI YGGCCR 1 cut(s) 303
Eam1104I CTCTTC 1 cut(s) 33
EarI CTCTTC 1 cut(s) 33
EciI GGCGGA 2 cut(s) 350, 926
Eco130I CCWWGG 3 cut(s) 300, 1092, 1165
Eco31I GGTCTC 2 cut(s) 25, 193
Eco57I CTGAAG 3 cut(s) 506, 668, 1365
Eco72I CACGTG 1 cut(s) 1222
Eco88I CYCGRG 1 cut(s) 1118
EcoNI CCTNNNNNAGG 1 cut(s) 1006
EcoRI GAATTC 1 cut(s) 933
EcoRII CCWGG 1 cut(s) 1132
EcoT14I CCWWGG 3 cut(s) 300, 1092, 1165
ErhI CCWWGG 3 cut(s) 300, 1092, 1165
FaeI CATG 6 cut(s) 244, 298, 443, 760, 775, 1096
FalI AAGNNNNNCTT 6 cut(s) 641, 673, 1245, 1277, 1363, 1395
FaqI GGGAC 1 cut(s) 208
FatI CATG 6 cut(s) 240, 294, 439, 756, 771, 1092
FauI CCCGC 1 cut(s) 249
FbaI TGATCA 2 cut(s) 733, 774
Fnu4HI GCNGC 2 cut(s) 1259, 1403
FokI GGATG 1 cut(s) 887
Fsp4HI GCNGC 2 cut(s) 1259, 1403
FspBI CTAG 1 cut(s) 813
GluI GCNGC 2 cut(s) 1259, 1403
HaeIII GGCC 1 cut(s) 305
HapII CCGG 4 cut(s) 180, 272, 331, 715
HgaI GACGC 1 cut(s) 210
Hin1II CATG 6 cut(s) 244, 298, 443, 760, 775, 1096
HindIII AAGCTT 2 cut(s) 641, 967
HinfI GANTC 8 cut(s) 244, 260, 268, 373, 445, 466, 623, 667
HpaII CCGG 4 cut(s) 180, 272, 331, 715
HphI GGTGA 3 cut(s) 15, 538, 1201
Hpy166II GTNNAC 5 cut(s) 369, 769, 1024, 1219, 1225
Hpy188I TCNGA 5 cut(s) 328, 648, 738, 892, 1412
Hpy188III TCNNGA 7 cut(s) 105, 173, 547, 686, 834, 941, 1118
Hpy8I GTNNAC 5 cut(s) 369, 769, 1024, 1219, 1225
HpyAV CCTTC 3 cut(s) 692, 831, 865
HpyCH4III ACNGT 7 cut(s) 68, 102, 116, 417, 1229, 1353, 1366
HpyCH4IV ACGT 4 cut(s) 512, 784, 924, 1221
HpyF10VI GCNNNNNNNGC 3 cut(s) 966, 975, 1308
HpyF3I CTNAG 7 cut(s) 350, 627, 672, 889, 956, 974, 1370
HpySE526I ACGT 4 cut(s) 512, 784, 924, 1221
Hsp92II CATG 6 cut(s) 244, 298, 443, 760, 775, 1096
Ksp22I TGATCA 2 cut(s) 733, 774
Kzo9I GATC 3 cut(s) 733, 774, 862
LmnI GCTCC 1 cut(s) 457
Lsp1109I GCAGC 2 cut(s) 1270, 1389
LweI GCATC 1 cut(s) 399
MaeI CTAG 1 cut(s) 813
MaeII ACGT 4 cut(s) 512, 784, 924, 1221
MaeIII GTNAC 1 cut(s) 1360
MalI GATC 3 cut(s) 735, 776, 864
MbiI CCGCTC 1 cut(s) 256
MboI GATC 3 cut(s) 733, 774, 862
MflI RGATCY 1 cut(s) 862
MhlI GDGCHC 1 cut(s) 1227
MlsI TGGCCA 1 cut(s) 305
MluNI TGGCCA 1 cut(s) 305
MlyI GAGTC 1 cut(s) 269
MmeI TCCRAC 2 cut(s) 351, 706
Mox20I TGGCCA 1 cut(s) 305
MroXI GAANNNNTTC 3 cut(s) 653, 933, 1076
MscI TGGCCA 1 cut(s) 305
MseI TTAA 5 cut(s) 141, 344, 963, 1139, 1199
Msp20I TGGCCA 1 cut(s) 305
MspI CCGG 4 cut(s) 180, 272, 331, 715
MspR9I CCNGG 3 cut(s) 180, 332, 1134
MvaI CCWGG 1 cut(s) 1134
MvnI CGCG 1 cut(s) 221
MwoI GCNNNNNNNGC 3 cut(s) 966, 975, 1308
NciI CCSGG 2 cut(s) 180, 332
NcoI CCATGG 1 cut(s) 1092
NdeII GATC 3 cut(s) 733, 774, 862
NlaIII CATG 6 cut(s) 244, 298, 443, 760, 775, 1096
NspV TTCGAA 1 cut(s) 247
PdmI GAANNNNTTC 3 cut(s) 653, 933, 1076
PfeI GAWTC 7 cut(s) 244, 268, 373, 445, 466, 623, 667
PflFI GACNNNGTC 1 cut(s) 1154
PkrI GCNGC 2 cut(s) 1260, 1404
PleI GAGTC 1 cut(s) 268
PmaCI CACGTG 1 cut(s) 1222
PmlI CACGTG 1 cut(s) 1222
PpsI GAGTC 1 cut(s) 268
Ppu21I YACGTR 2 cut(s) 513, 1222
Psp6I CCWGG 1 cut(s) 1132
PspCI CACGTG 1 cut(s) 1222
PspGI CCWGG 1 cut(s) 1132
PstI CTGCAG 1 cut(s) 198
PsuI RGATCY 1 cut(s) 862
PsyI GACNNNGTC 1 cut(s) 1154
RsaI GTAC 6 cut(s) 368, 438, 492, 515, 704, 1039
RsaNI GTAC 6 cut(s) 367, 437, 491, 514, 703, 1038
SaqAI TTAA 5 cut(s) 141, 344, 963, 1139, 1199
SatI GCNGC 2 cut(s) 1259, 1403
Sau3AI GATC 3 cut(s) 733, 774, 862
SchI GAGTC 1 cut(s) 269
ScrFI CCNGG 3 cut(s) 180, 332, 1134
SduI GDGCHC 1 cut(s) 1227
SfaNI GCATC 1 cut(s) 399
SfcI CTRYAG 3 cut(s) 194, 1176, 1279
SfuI TTCGAA 1 cut(s) 247
SmlI CTYRAG 1 cut(s) 278
SmoI CTYRAG 1 cut(s) 278
SsiI CCGC 5 cut(s) 219, 256, 335, 911, 1339
SspI AATATT 2 cut(s) 1065, 1087
SspMI CTAG 1 cut(s) 813
StyD4I CCNGG 3 cut(s) 178, 330, 1132
StyI CCWWGG 3 cut(s) 300, 1092, 1165
TaaI ACNGT 7 cut(s) 68, 102, 116, 417, 1229, 1353, 1366
TaiI ACGT 4 cut(s) 515, 787, 927, 1224
TaqI TCGA 2 cut(s) 247, 399
TatI WGTACW 3 cut(s) 366, 490, 702
TfiI GAWTC 7 cut(s) 244, 268, 373, 445, 466, 623, 667
Tru1I TTAA 5 cut(s) 141, 344, 963, 1139, 1199
Tru9I TTAA 5 cut(s) 141, 344, 963, 1139, 1199
TscAI CASTG 3 cut(s) 270, 1232, 1356
TseI GCWGC 2 cut(s) 1258, 1402
TspDTI ATGAA 5 cut(s) 576, 636, 745, 894, 1223
TspGWI ACGGA 1 cut(s) 145
TspRI CASTG 3 cut(s) 270, 1232, 1356
Tth111I GACNNNGTC 1 cut(s) 1154
VneI GTGCAC 1 cut(s) 1223
XagI CCTNNNNNAGG 1 cut(s) 1006
XapI RAATTY 3 cut(s) 184, 502, 933
XcmI CCANNNNNNNNNTGG 1 cut(s) 1099
XmnI GAANNNNTTC 3 cut(s) 653, 933, 1076
XspI CTAG 1 cut(s) 813
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.