MD09G1035500.v1.1

Protein of unknown function (DUF3754)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000718
Physical Location & Seq
Forward (+)
2171837 .. 2177391
5555 bp
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UTR
Exon/CDS
Intron
MD09G1035500.v1.1.491

Sequence Viewer

Length: 1464 bp
ATGGAGCAGAAGAAGAAGGATATGATTCGAATAGAGCGCGAATCCGTCATCCCGATCATCAAGCCCAAGATTATCATGACCTTGGCCAACCTCATTGAACATAGTTCCGACCGGGCGGAGTTTTTGAAGTTGTGCAAGAGAGTTGAGTACACAATTCGGGCTTGGTATCTTCTGCAATTCGAGGATTTGATGCAATTGTACTCCCTCTTTGACCCTGTAAATGGGGCTAAAAAATTGGAGCAGCAGAACTTTTCCGTGAAAGAAGTTGATGTGCTTGAGCAGAATTTCTTGAAGTACTTATTTCAGGTGATGGAGAAGAGTAATTTCAATATAACAAGTAATGAGGAAATTGAGGTTGCACGTTCAGGCCAGTATCTTCTAAATCTACCCATCACAGTTGACGAATCTAAGCTTGATAAGAAGCTCTTAAAGAATTATTTCGCTGAGCATCCTCAACCAAACCTTCCAGAGTTTGCTGATAAGTACATTATCTTCCGGCGTGGCGTTGGACTTGATCAGACAACTGATTACTTTTTCATGGAGAAAGTGGACATGATGATTGCGCGCTTTTGGGCATATCTTTTACGAATAACTAGGATCGAAAAACTTTTATCCAGATGGTCAAGTGGACGACCTAAGTCTGATCCAAAAAAGAATGATGAGATTGTCTCTGGAGCAGATGGAGAAGATGAAGACTTATTTATTGAAAGAATCCGTCTAGAAAAAATGGAACTAAGTGTTAAAAATTTGCTGAGCAAGACGACAATCAAAGAGCCAACCTTTGATAGGATAATTGTTGTTTACAGGCGGGCAAGTACCAAAGCCAAAGAAGAAAGGGGAATATATGTAAAGCACTTCAGAAATATTCCGATGGCTGATATGGAGATAGTTCTTCCTGAGAAGAAGAACCCGGGATTAACTCCAATGGACTGGGTCAAGTTCCTTGTCTCTGCTGTAGTTGGGCTGGTTGCCGTTGTTGGTTCGGTTGAAATGCCTAAAGCTGATCTTTGGGTCATTATTGCAATTCTTTCCACTGTGATTGGTTACTGTGCTAAGATATACTTCACGTTTCAGCAGAACTTGGTTGCATATCAGAACTTGATAACACAGTCCATGTATGACAAACAACTAGATAGCGGAAGGGGCACTCTTCTGCACTTGTGTGATGATGTGATTCAACAGGAAGTCAAGGAGGTGATAATTGCTTACTATATATTAATGGAGCAAGGGAAAGCTACTTTAAAAGACCTGGATATGTGGTGTGAGGAACTAATTAAAACCGAGTTTAACGCCAATTGCAATTTCGAGGTGGAGGATGCAGTTCAGAAGTTGGTGAAATTGAAAATTGTTGCTCAGGATTCTGTTGGACGGTTTTACTGCGTTGGGCTGAAGCGTGCTAACGAGATCATTGGCACCACCACAGAGGAGCTTGTCCTCAAGGCAAAACAAGGTGGTAATGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3754

Protein Analysis

488

Amino Acids

56.38

Weight (kDa)

6.26

Isoelectric Point (pI)

33.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3754 PF12576 261 - 382 1.2e-24 Protein of unknown function (DUF3754)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1412
AccII CGCG 2 cut(s) 39, 565
AciI CCGC 3 cut(s) 116, 808, 1137
AclWI GGATC 2 cut(s) 605, 638
AcoI YGGCCR 1 cut(s) 84
AcsI RAATTY 2 cut(s) 283, 745
AcuI CTGAAG 2 cut(s) 841, 1409
AfaI GTAC 5 cut(s) 149, 200, 296, 485, 817
AfiI CCNNNNNNNGG 2 cut(s) 221, 786
AgsI TTSAA 8 cut(s) 98, 127, 292, 328, 707, 989, 1178, 1342
AjnI CCWGG 1 cut(s) 1248
AjuI GAANNNNNNNTTGG 2 cut(s) 1286, 1318
AleI CACNNNNGTG 1 cut(s) 1161
AluBI AGCT 5 cut(s) 412, 424, 1001, 1235, 1429
AluI AGCT 5 cut(s) 412, 424, 1001, 1235, 1429
Alw26I GTCTC 2 cut(s) 673, 952
AlwI GGATC 2 cut(s) 605, 638
Ama87I CYCGRG 1 cut(s) 910
AoxI GGCC 2 cut(s) 84, 367
ApeKI GCWGC 1 cut(s) 241
ApoI RAATTY 2 cut(s) 283, 745
AseI ATTAAT 1 cut(s) 1217
Asp700I GAANNNNTTC 1 cut(s) 437
AspLEI GCGC 3 cut(s) 39, 565, 567
AsuC2I CCSGG 3 cut(s) 113, 911, 912
AsuHPI GGTGA 3 cut(s) 319, 1207, 1345
AsuII TTCGAA 1 cut(s) 28
AvaI CYCGRG 1 cut(s) 910
BaeGI GKGCMC 1 cut(s) 1148
BalI TGGCCA 1 cut(s) 86
BanI GGYRCC 1 cut(s) 1412
BarI GAAGNNNNNNTAC 2 cut(s) 476, 508
BbsI GAAGAC 1 cut(s) 699
BbvI GCAGC 1 cut(s) 253
BccI CCATC 5 cut(s) 304, 398, 612, 674, 865
BceAI ACGGC 1 cut(s) 956
BciT130I CCWGG 1 cut(s) 1250
BclI TGATCA 1 cut(s) 514
BcnI CCSGG 3 cut(s) 113, 911, 912
BcoDI GTCTC 2 cut(s) 673, 952
BfaI CTAG 3 cut(s) 594, 719, 1130
BfmI CTRYAG 1 cut(s) 954
BisI GCNGC 1 cut(s) 242
BlpI GCTNAGC 2 cut(s) 444, 752
BlsI GCNGC 1 cut(s) 243
BmcAI AGTACT 1 cut(s) 296
Bme1390I CCNGG 4 cut(s) 113, 911, 912, 1250
BmeT110I CYCGRG 1 cut(s) 910
BmiI GGNNCC 1 cut(s) 1414
BmrFI CCNGG 4 cut(s) 113, 911, 912, 1250
BmrI ACTGGG 1 cut(s) 940
BmsI GCATC 3 cut(s) 180, 457, 1306
BmuI ACTGGG 1 cut(s) 940
BoxI GACNNNNGTC 1 cut(s) 637
BpiI GAAGAC 1 cut(s) 699
BplI GAGNNNNNCTC 2 cut(s) 653, 685
BpmI CTGGAG 1 cut(s) 693
Bpu10I CCTNAGC 1 cut(s) 1353
Bpu1102I GCTNAGC 2 cut(s) 444, 752
Bpu14I TTCGAA 1 cut(s) 28
BpuEI CTTGAG 2 cut(s) 296, 1421
BpuMI CCSGG 3 cut(s) 113, 911, 912
BsaJI CCNNGG 2 cut(s) 81, 910
Bsc4I CCNNNNNNNGG 2 cut(s) 221, 786
Bse1I ACTGG 2 cut(s) 370, 935
BseBI CCWGG 1 cut(s) 1250
BseDI CCNNGG 2 cut(s) 81, 910
BseGI GGATG 3 cut(s) 48, 448, 1321
BseLI CCNNNNNNNGG 2 cut(s) 221, 786
BseMII CTCAG 4 cut(s) 435, 743, 888, 1367
BseNI ACTGG 2 cut(s) 370, 935
BsePI GCGCGC 1 cut(s) 563
BseRI GAGGAG 1 cut(s) 1439
BseSI GKGCMC 1 cut(s) 1148
BseXI GCAGC 1 cut(s) 253
BsgI GTGCAG 1 cut(s) 1139
Bsh1236I CGCG 2 cut(s) 39, 565
Bsh1285I CGRYCG 1 cut(s) 112
BshFI GGCC 2 cut(s) 86, 369
BshNI GGYRCC 1 cut(s) 1412
BsiEI CGRYCG 1 cut(s) 112
BsiHKCI CYCGRG 1 cut(s) 910
BsiSI CCGG 3 cut(s) 112, 496, 911
BslI CCNNNNNNNGG 2 cut(s) 221, 786
BsmAI GTCTC 2 cut(s) 673, 952
BsnI GGCC 2 cut(s) 86, 369
BsoBI CYCGRG 1 cut(s) 910
Bsp119I TTCGAA 1 cut(s) 28
Bsp1286I GDGCHC 1 cut(s) 1148
Bsp143I GATC 6 cut(s) 54, 514, 597, 643, 1003, 1404
Bsp1720I GCTNAGC 2 cut(s) 444, 752
BspACI CCGC 3 cut(s) 116, 808, 1137
BspANI GGCC 2 cut(s) 86, 369
BspCNI CTCAG 4 cut(s) 436, 744, 889, 1366
BspFNI CGCG 2 cut(s) 39, 565
BspHI TCATGA 1 cut(s) 75
BspLI GGNNCC 1 cut(s) 1414
BspPI GGATC 2 cut(s) 605, 638
BspT104I TTCGAA 1 cut(s) 28
BspT107I GGYRCC 1 cut(s) 1412
BsrI ACTGG 2 cut(s) 370, 935
BssECI CCNNGG 2 cut(s) 81, 910
BssHII GCGCGC 1 cut(s) 563
BssMI GATC 6 cut(s) 54, 514, 597, 643, 1003, 1404
BssT1I CCWWGG 1 cut(s) 81
Bst2UI CCWGG 1 cut(s) 1250
Bst4CI ACNGT 5 cut(s) 397, 1036, 1049, 1110, 1371
Bst6I CTCTTC 2 cut(s) 311, 1155
BstBI TTCGAA 1 cut(s) 28
BstC8I GCNNGC 3 cut(s) 565, 810, 1395
BstDEI CTNAG 8 cut(s) 408, 444, 636, 734, 752, 897, 1053, 1353
BstENI CCTNNNNNAGG 1 cut(s) 784
BstF5I GGATG 3 cut(s) 48, 448, 1321
BstFNI CGCG 2 cut(s) 39, 565
BstHHI GCGC 3 cut(s) 39, 565, 567
BstKTI GATC 6 cut(s) 57, 517, 600, 646, 1006, 1407
BstMAI GTCTC 2 cut(s) 673, 952
BstMBI GATC 6 cut(s) 54, 514, 597, 643, 1003, 1404
BstMCI CGRYCG 1 cut(s) 112
BstMWI GCNNNNNNNGC 1 cut(s) 1143
BstNI CCWGG 1 cut(s) 1250
BstPAI GACNNNNGTC 1 cut(s) 637
BstSCI CCNGG 4 cut(s) 111, 909, 910, 1248
BstSFI CTRYAG 1 cut(s) 954
BstSLI GKGCMC 1 cut(s) 1148
BstUI CGCG 2 cut(s) 39, 565
BstV1I GCAGC 1 cut(s) 253
BstV2I GAAGAC 1 cut(s) 699
BstXI CCANNNNNNTGG 1 cut(s) 930
BsuRI GGCC 2 cut(s) 86, 369
BtsCI GGATG 3 cut(s) 48, 448, 1321
BtsIMutI CAGTG 1 cut(s) 1032
Cac8I GCNNGC 3 cut(s) 565, 810, 1395
CciI TCATGA 1 cut(s) 75
CfoI GCGC 3 cut(s) 39, 565, 567
Cfr9I CCCGGG 1 cut(s) 910
Csp6I GTAC 5 cut(s) 148, 199, 295, 484, 816
CspCI CAANNNNNGTGG 2 cut(s) 1021, 1056
CviAII CATG 4 cut(s) 76, 538, 553, 1114
CviQI GTAC 5 cut(s) 148, 199, 295, 484, 816
DdeI CTNAG 8 cut(s) 408, 444, 636, 734, 752, 897, 1053, 1353
DpnI GATC 6 cut(s) 56, 516, 599, 645, 1005, 1406
DpnII GATC 6 cut(s) 54, 514, 597, 643, 1003, 1404
DraI TTTAAA 1 cut(s) 1242
EaeI YGGCCR 1 cut(s) 84
Eam1104I CTCTTC 2 cut(s) 311, 1155
EarI CTCTTC 2 cut(s) 311, 1155
EciI GGCGGA 1 cut(s) 131
Eco130I CCWWGG 1 cut(s) 81
Eco57I CTGAAG 2 cut(s) 841, 1409
Eco88I CYCGRG 1 cut(s) 910
EcoNI CCTNNNNNAGG 1 cut(s) 784
EcoRII CCWGG 1 cut(s) 1248
EcoT14I CCWWGG 1 cut(s) 81
ErhI CCWWGG 1 cut(s) 81
FaeI CATG 4 cut(s) 79, 541, 556, 1117
FalI AAGNNNNNCTT 6 cut(s) 410, 442, 990, 1022, 1046, 1078
FatI CATG 4 cut(s) 75, 537, 552, 1113
FauI CCCGC 1 cut(s) 801
FbaI TGATCA 1 cut(s) 514
Fnu4HI GCNGC 1 cut(s) 242
FokI GGATG 3 cut(s) 35, 435, 1328
Fsp4HI GCNGC 1 cut(s) 242
FspBI CTAG 3 cut(s) 594, 719, 1130
GlaI GCGC 3 cut(s) 38, 564, 566
GluI GCNGC 1 cut(s) 242
GsuI CTGGAG 1 cut(s) 693
HaeIII GGCC 2 cut(s) 86, 369
HapII CCGG 3 cut(s) 112, 496, 911
HhaI GCGC 3 cut(s) 39, 565, 567
Hin1II CATG 4 cut(s) 79, 541, 556, 1117
Hin6I GCGC 3 cut(s) 37, 563, 565
HinP1I GCGC 3 cut(s) 37, 563, 565
HincII GTYRAC 1 cut(s) 400
HindII GTYRAC 1 cut(s) 400
HindIII AAGCTT 1 cut(s) 410
HinfI GANTC 6 cut(s) 25, 41, 404, 711, 1174, 1358
HpaII CCGG 3 cut(s) 112, 496, 911
HphI GGTGA 3 cut(s) 319, 1207, 1345
Hpy166II GTNNAC 5 cut(s) 150, 400, 550, 629, 802
Hpy188I TCNGA 7 cut(s) 109, 519, 643, 860, 870, 1095, 1326
Hpy188III TCNNGA 9 cut(s) 52, 76, 289, 467, 615, 672, 719, 896, 1355
Hpy8I GTNNAC 5 cut(s) 150, 400, 550, 629, 802
HpyAV CCTTC 3 cut(s) 10, 473, 1134
HpyCH4III ACNGT 5 cut(s) 397, 1036, 1049, 1110, 1371
HpyCH4IV ACGT 2 cut(s) 361, 1067
HpyCH4V TGCA 9 cut(s) 135, 175, 193, 359, 1022, 1088, 1156, 1299, 1319
HpyF10VI GCNNNNNNNGC 1 cut(s) 1143
HpyF3I CTNAG 8 cut(s) 408, 444, 636, 734, 752, 897, 1053, 1353
HpySE526I ACGT 2 cut(s) 361, 1067
Hsp92II CATG 4 cut(s) 79, 541, 556, 1117
HspAI GCGC 3 cut(s) 37, 563, 565
Ksp22I TGATCA 1 cut(s) 514
Kzo9I GATC 6 cut(s) 54, 514, 597, 643, 1003, 1404
LmnI GCTCC 5 cut(s) 4, 238, 674, 1222, 1426
Lsp1109I GCAGC 1 cut(s) 253
LweI GCATC 3 cut(s) 180, 457, 1306
MaeI CTAG 3 cut(s) 594, 719, 1130
MaeII ACGT 2 cut(s) 361, 1067
MaeIII GTNAC 1 cut(s) 1043
MalI GATC 6 cut(s) 56, 516, 599, 645, 1005, 1406
MboI GATC 6 cut(s) 54, 514, 597, 643, 1003, 1404
MfeI CAATTG 2 cut(s) 194, 1294
MhlI GDGCHC 1 cut(s) 1148
MlsI TGGCCA 1 cut(s) 86
MluNI TGGCCA 1 cut(s) 86
MmeI TCCRAC 3 cut(s) 132, 487, 1345
Mox20I TGGCCA 1 cut(s) 86
MroXI GAANNNNTTC 1 cut(s) 437
MscI TGGCCA 1 cut(s) 86
MseI TTAA 7 cut(s) 428, 741, 917, 1217, 1241, 1275, 1287
MslI CAYNNNNRTG 1 cut(s) 1161
Msp20I TGGCCA 1 cut(s) 86
MspI CCGG 3 cut(s) 112, 496, 911
MspR9I CCNGG 4 cut(s) 113, 911, 912, 1250
MunI CAATTG 2 cut(s) 194, 1294
MvaI CCWGG 1 cut(s) 1250
MvnI CGCG 2 cut(s) 39, 565
MwoI GCNNNNNNNGC 1 cut(s) 1143
NciI CCSGG 3 cut(s) 113, 911, 912
NdeII GATC 6 cut(s) 54, 514, 597, 643, 1003, 1404
NlaIII CATG 4 cut(s) 79, 541, 556, 1117
NlaIV GGNNCC 1 cut(s) 1414
NspV TTCGAA 1 cut(s) 28
OliI CACNNNNGTG 1 cut(s) 1161
PagI TCATGA 1 cut(s) 75
PauI GCGCGC 1 cut(s) 563
PdmI GAANNNNTTC 1 cut(s) 437
PfeI GAWTC 6 cut(s) 25, 41, 404, 711, 1174, 1358
PflFI GACNNNGTC 1 cut(s) 932
PkrI GCNGC 1 cut(s) 243
PshAI GACNNNNGTC 1 cut(s) 637
PshBI ATTAAT 1 cut(s) 1217
Psp6I CCWGG 1 cut(s) 1248
PspGI CCWGG 1 cut(s) 1248
PspN4I GGNNCC 1 cut(s) 1414
PsyI GACNNNGTC 1 cut(s) 932
PteI GCGCGC 1 cut(s) 563
RsaI GTAC 5 cut(s) 149, 200, 296, 485, 817
RsaNI GTAC 5 cut(s) 148, 199, 295, 484, 816
RseI CAYNNNNRTG 1 cut(s) 1161
SaqAI TTAA 7 cut(s) 428, 741, 917, 1217, 1241, 1275, 1287
SatI GCNGC 1 cut(s) 242
Sau3AI GATC 6 cut(s) 54, 514, 597, 643, 1003, 1404
ScaI AGTACT 1 cut(s) 296
ScrFI CCNGG 4 cut(s) 113, 911, 912, 1250
SduI GDGCHC 1 cut(s) 1148
SfaNI GCATC 3 cut(s) 180, 457, 1306
SfcI CTRYAG 1 cut(s) 954
SfuI TTCGAA 1 cut(s) 28
SmaI CCCGGG 1 cut(s) 912
SmiMI CAYNNNNRTG 1 cut(s) 1161
SmlI CTYRAG 2 cut(s) 275, 1436
SmoI CTYRAG 2 cut(s) 275, 1436
SsiI CCGC 3 cut(s) 116, 808, 1137
SspI AATATT 1 cut(s) 865
SspMI CTAG 3 cut(s) 594, 719, 1130
StyD4I CCNGG 4 cut(s) 111, 909, 910, 1248
StyI CCWWGG 1 cut(s) 81
TaaI ACNGT 5 cut(s) 397, 1036, 1049, 1110, 1371
TaiI ACGT 2 cut(s) 364, 1070
TaqI TCGA 4 cut(s) 28, 180, 600, 1305
TatI WGTACW 4 cut(s) 147, 198, 294, 483
TfiI GAWTC 6 cut(s) 25, 41, 404, 711, 1174, 1358
Tru1I TTAA 7 cut(s) 428, 741, 917, 1217, 1241, 1275, 1287
Tru9I TTAA 7 cut(s) 428, 741, 917, 1217, 1241, 1275, 1287
TscAI CASTG 1 cut(s) 1039
TseI GCWGC 1 cut(s) 241
TspDTI ATGAA 2 cut(s) 526, 705
TspGWI ACGGA 3 cut(s) 34, 244, 704
TspMI CCCGGG 1 cut(s) 910
TspRI CASTG 1 cut(s) 1039
Tth111I GACNNNGTC 1 cut(s) 932
VspI ATTAAT 1 cut(s) 1217
XagI CCTNNNNNAGG 1 cut(s) 784
XapI RAATTY 2 cut(s) 283, 745
XbaI TCTAGA 1 cut(s) 718
XmaI CCCGGG 1 cut(s) 910
XmnI GAANNNNTTC 1 cut(s) 437
XspI CTAG 3 cut(s) 594, 719, 1130
ZrmI AGTACT 1 cut(s) 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.