RchiOBHm_Chr2g0176581

Protein of unknown function (DUF3754)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000718
Physical Location & Seq
Forward (+)
88334863 .. 88338295
3433 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ54358

Sequence Viewer

Length: 1131 bp
ATGGGCAAGGGCAAGGGCAAGAAAGACATTATTCGATTAGAGCGTGAATCCGTCATTGCAGTTATTAAGCCCAGACTCATCATGACCTTGACCAATCTCATCGAGCAAAGTTCTGATCGGACTGAGTTTTTGAAGCTATGCAAGAGGGTCGAGTACACAATTCGGGCTTGGTATCTTCTACAATTTGAGGATTTGATGCAACTGTACTCCCTCTTTGATCCTGTACATGGAGCCCAGAGGTTGGAGCAACAGAAACTTGACGATGAAGAAATTGATGTGCTTGAACAAAATTTCCTCATGTACTTATTTCAGGTGATGGAAAAGAGTAATTTTAAGATTGCAAATAATGAAGAAATTGAGGTTGCGGCTTCAGGGCAGTATCTTCTACGTCTTCCCATCGTGGTTGATGAGTCTAAGCTTGACAACAGACTTTTGAAGAAATATTTCGAAGGTCATCCTACCGAAGACATTCCAGATTTTGCTGATAAGTATGTCATCTTCCGACGCGGCATTGGACTTGATAAGACAACCGGTTGGTTTTTCATGGAGAAGGTGGACATTATCATTGCACGTTTTTGGAAATATCTTTTACAAATAACAAAGTTAGACAAGTTTTTCAAGAAACCAACCCCTAAGAAGAGGGATTCAGAATCTCATACAGATGAGGACGATTTCTTTGTTGAACGAATCCGTCTTGAAAACATGGAACTAAGCGTTCAAACTTTGCTTCTTAAGAACACAATCCAGGAGCCAACTTTTGAAAGGATTATCGTTGTTTACAGACAAGCAACTACCGACGAGGATGGTTCAGAACGGGGAATACATATTAAACATTTCAAAAATATTCCAATGGCTGATTTGGAGATAGTTCTTCCTGAAAAGAAGAACCCAGGTTTAACACCAATGGACTGGCTCTCGTTCATAGGCTCTGCTGTAGTTGGTCTGGTTGCTGTTATTACTTCGATTGAAACGACTAAAGTCGATCTTTGGGTTATGTTTGCAATTCTTTCCACTGTGATTGGTTACTGTGCTAAGACATATTTCAGGTTGCTCTTCTTTTCTAGTTCATGTTTAAGTATATTGGTATTGAGAGAGATTGATGAGAGAAGTGTGTTTCATTATAGAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3754

Protein Analysis

376

Amino Acids

44.07

Weight (kDa)

5.73

Isoelectric Point (pI)

31.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3754 PF12576 253 - 350 4.9e-16 Protein of unknown function (DUF3754)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 241
AccII CGCG 1 cut(s) 507
AciI CCGC 2 cut(s) 365, 507
AclWI GGATC 1 cut(s) 212
AcsI RAATTY 1 cut(s) 289
AcuI CTGAAG 1 cut(s) 354
AfaI GTAC 4 cut(s) 155, 206, 225, 302
AfiI CCNNNNNNNGG 2 cut(s) 227, 241
AflII CTTAAG 1 cut(s) 731
AgeI ACCGGT 1 cut(s) 530
AjnI CCWGG 2 cut(s) 744, 889
AloI GAACNNNNNNTCC 2 cut(s) 276, 308
AluBI AGCT 2 cut(s) 136, 418
AluI AGCT 2 cut(s) 136, 418
AlwI GGATC 1 cut(s) 212
ApoI RAATTY 1 cut(s) 289
ArsI GACNNNNNNTTYG 4 cut(s) 112, 144, 659, 691
AsiGI ACCGGT 1 cut(s) 530
Asp700I GAANNNNTTC 2 cut(s) 443, 468
AsuHPI GGTGA 1 cut(s) 325
AsuII TTCGAA 1 cut(s) 447
BanII GRGCYC 1 cut(s) 235
BarI GAAGNNNNNNTAC 2 cut(s) 482, 514
BbsI GAAGAC 2 cut(s) 383, 471
BccI CCATC 3 cut(s) 310, 404, 797
BcgI CGANNNNNNTGC 2 cut(s) 130, 164
BciT130I CCWGG 2 cut(s) 746, 891
BfaI CTAG 1 cut(s) 1062
BfmI CTRYAG 1 cut(s) 933
BfrI CTTAAG 1 cut(s) 731
BisI GCNGC 2 cut(s) 366, 508
BlsI GCNGC 2 cut(s) 367, 509
Bme1390I CCNGG 2 cut(s) 746, 891
BmiI GGNNCC 2 cut(s) 232, 750
BmrFI CCNGG 2 cut(s) 746, 891
BmsI GCATC 1 cut(s) 186
BoxI GACNNNNGTC 1 cut(s) 977
BpiI GAAGAC 2 cut(s) 383, 471
Bpu14I TTCGAA 1 cut(s) 447
BsaJI CCNNGG 1 cut(s) 889
BsaWI WCCGGW 1 cut(s) 530
Bsc4I CCNNNNNNNGG 2 cut(s) 227, 241
Bse118I RCCGGY 1 cut(s) 530
Bse1I ACTGG 1 cut(s) 914
Bse3DI GCAATG 2 cut(s) 54, 564
BseBI CCWGG 2 cut(s) 746, 891
BseDI CCNNGG 1 cut(s) 889
BseGI GGATG 2 cut(s) 454, 808
BseLI CCNNNNNNNGG 2 cut(s) 227, 241
BseMI GCAATG 2 cut(s) 54, 564
BseMII CTCAG 1 cut(s) 114
BseNI ACTGG 1 cut(s) 914
Bsh1236I CGCG 1 cut(s) 507
BshTI ACCGGT 1 cut(s) 530
BsiSI CCGG 1 cut(s) 531
BslI CCNNNNNNNGG 2 cut(s) 227, 241
Bsp119I TTCGAA 1 cut(s) 447
Bsp1286I GDGCHC 1 cut(s) 235
Bsp1407I TGTACA 1 cut(s) 223
Bsp143I GATC 3 cut(s) 115, 217, 982
BspACI CCGC 2 cut(s) 365, 507
BspCNI CTCAG 1 cut(s) 115
BspFNI CGCG 1 cut(s) 507
BspHI TCATGA 1 cut(s) 81
BspLI GGNNCC 2 cut(s) 232, 750
BspPI GGATC 1 cut(s) 212
BspQI GCTCTTC 1 cut(s) 1058
BspT104I TTCGAA 1 cut(s) 447
BspTI CTTAAG 1 cut(s) 731
BsrDI GCAATG 2 cut(s) 54, 564
BsrFI RCCGGY 1 cut(s) 530
BsrGI TGTACA 1 cut(s) 223
BsrI ACTGG 1 cut(s) 914
BssAI RCCGGY 1 cut(s) 530
BssECI CCNNGG 1 cut(s) 889
BssMI GATC 3 cut(s) 115, 217, 982
Bst2UI CCWGG 2 cut(s) 746, 891
Bst4CI ACNGT 3 cut(s) 204, 1015, 1028
Bst6I CTCTTC 2 cut(s) 632, 1058
BstAFI CTTAAG 1 cut(s) 731
BstAUI TGTACA 1 cut(s) 223
BstBI TTCGAA 1 cut(s) 447
BstDEI CTNAG 5 cut(s) 123, 414, 633, 710, 1032
BstF5I GGATG 2 cut(s) 454, 808
BstFNI CGCG 1 cut(s) 507
BstKTI GATC 3 cut(s) 118, 220, 985
BstMBI GATC 3 cut(s) 115, 217, 982
BstNI CCWGG 2 cut(s) 746, 891
BstPAI GACNNNNGTC 1 cut(s) 977
BstSCI CCNGG 2 cut(s) 744, 889
BstSFI CTRYAG 1 cut(s) 933
BstUI CGCG 1 cut(s) 507
BstV2I GAAGAC 2 cut(s) 383, 471
BstXI CCANNNNNNTGG 1 cut(s) 909
BtsCI GGATG 2 cut(s) 454, 808
BtsIMutI CAGTG 1 cut(s) 1011
CciI TCATGA 1 cut(s) 81
Cfr10I RCCGGY 1 cut(s) 530
CseI GACGC 1 cut(s) 513
Csp6I GTAC 4 cut(s) 154, 205, 224, 301
CspAI ACCGGT 1 cut(s) 530
CspCI CAANNNNNGTGG 2 cut(s) 1000, 1035
CviAII CATG 6 cut(s) 82, 227, 298, 544, 703, 1068
CviQI GTAC 4 cut(s) 154, 205, 224, 301
DdeI CTNAG 5 cut(s) 123, 414, 633, 710, 1032
DpnI GATC 3 cut(s) 117, 219, 984
DpnII GATC 3 cut(s) 115, 217, 982
Eam1104I CTCTTC 2 cut(s) 632, 1058
EarI CTCTTC 2 cut(s) 632, 1058
Eco24I GRGCYC 1 cut(s) 235
Eco57I CTGAAG 1 cut(s) 354
EcoRII CCWGG 2 cut(s) 744, 889
EcoT38I GRGCYC 1 cut(s) 235
FaeI CATG 6 cut(s) 85, 230, 301, 547, 706, 1071
FalI AAGNNNNNCTT 2 cut(s) 969, 1001
FatI CATG 6 cut(s) 81, 226, 297, 543, 702, 1067
Fnu4HI GCNGC 2 cut(s) 366, 508
FokI GGATG 2 cut(s) 441, 815
FriOI GRGCYC 1 cut(s) 235
Fsp4HI GCNGC 2 cut(s) 366, 508
FspBI CTAG 1 cut(s) 1062
GluI GCNGC 2 cut(s) 366, 508
HapII CCGG 1 cut(s) 531
HgaI GACGC 1 cut(s) 513
Hin1II CATG 6 cut(s) 85, 230, 301, 547, 706, 1071
HindIII AAGCTT 1 cut(s) 416
HinfI GANTC 6 cut(s) 47, 75, 410, 644, 650, 687
HpaII CCGG 1 cut(s) 531
HphI GGTGA 1 cut(s) 325
Hpy166II GTNNAC 3 cut(s) 156, 556, 778
Hpy188I TCNGA 5 cut(s) 115, 120, 503, 649, 811
Hpy188III TCNNGA 5 cut(s) 82, 473, 619, 695, 875
Hpy8I GTNNAC 3 cut(s) 156, 556, 778
Hpy99I CGWCG 2 cut(s) 507, 800
HpyAV CCTTC 2 cut(s) 443, 544
HpyCH4III ACNGT 3 cut(s) 204, 1015, 1028
HpyCH4IV ACGT 2 cut(s) 388, 571
HpyCH4V TGCA 6 cut(s) 59, 141, 199, 341, 569, 1001
HpyF3I CTNAG 5 cut(s) 123, 414, 633, 710, 1032
HpySE526I ACGT 2 cut(s) 388, 571
Hsp92II CATG 6 cut(s) 85, 230, 301, 547, 706, 1071
Kzo9I GATC 3 cut(s) 115, 217, 982
LguI GCTCTTC 1 cut(s) 1058
LmnI GCTCC 3 cut(s) 230, 244, 748
LweI GCATC 1 cut(s) 186
MaeI CTAG 1 cut(s) 1062
MaeII ACGT 2 cut(s) 388, 571
MaeIII GTNAC 1 cut(s) 1022
MalI GATC 3 cut(s) 117, 219, 984
MboI GATC 3 cut(s) 115, 217, 982
MhlI GDGCHC 1 cut(s) 235
MluCI AATT 7 cut(s) 159, 182, 270, 289, 328, 354, 1002
MlyI GAGTC 2 cut(s) 69, 419
MmeI TCCRAC 2 cut(s) 222, 526
MnlI CCTC 9 cut(s) 138, 181, 221, 231, 305, 352, 633, 658, 793
MroXI GAANNNNTTC 2 cut(s) 443, 468
MseI TTAA 6 cut(s) 66, 333, 732, 828, 896, 1073
MslI CAYNNNNRTG 1 cut(s) 660
MspCI CTTAAG 1 cut(s) 731
MspI CCGG 1 cut(s) 531
MspR9I CCNGG 2 cut(s) 746, 891
MvaI CCWGG 2 cut(s) 746, 891
MvnI CGCG 1 cut(s) 507
NdeII GATC 3 cut(s) 115, 217, 982
NlaIII CATG 6 cut(s) 85, 230, 301, 547, 706, 1071
NlaIV GGNNCC 2 cut(s) 232, 750
NspV TTCGAA 1 cut(s) 447
PagI TCATGA 1 cut(s) 81
PciSI GCTCTTC 1 cut(s) 1058
PcsI WCGNNNNNNNCGW 2 cut(s) 40, 968
PdmI GAANNNNTTC 2 cut(s) 443, 468
PfeI GAWTC 4 cut(s) 47, 644, 650, 687
PflMI CCANNNNNTGG 1 cut(s) 241
PfoI TCCNGGA 1 cut(s) 744
PinAI ACCGGT 1 cut(s) 530
PkrI GCNGC 2 cut(s) 367, 509
PleI GAGTC 2 cut(s) 69, 418
PpsI GAGTC 2 cut(s) 69, 418
PshAI GACNNNNGTC 1 cut(s) 977
Psp6I CCWGG 2 cut(s) 744, 889
PspGI CCWGG 2 cut(s) 744, 889
PspN4I GGNNCC 2 cut(s) 232, 750
PsrI GAACNNNNNNTAC 2 cut(s) 804, 836
RsaI GTAC 4 cut(s) 155, 206, 225, 302
RsaNI GTAC 4 cut(s) 154, 205, 224, 301
RseI CAYNNNNRTG 1 cut(s) 660
SapI GCTCTTC 1 cut(s) 1058
SaqAI TTAA 6 cut(s) 66, 333, 732, 828, 896, 1073
SatI GCNGC 2 cut(s) 366, 508
Sau3AI GATC 3 cut(s) 115, 217, 982
SchI GAGTC 2 cut(s) 69, 419
ScrFI CCNGG 2 cut(s) 746, 891
SduI GDGCHC 1 cut(s) 235
SfaNI GCATC 1 cut(s) 186
SfcI CTRYAG 1 cut(s) 933
SfuI TTCGAA 1 cut(s) 447
SmiMI CAYNNNNRTG 1 cut(s) 660
SmlI CTYRAG 1 cut(s) 731
SmoI CTYRAG 1 cut(s) 731
Sse9I AATT 7 cut(s) 159, 182, 270, 289, 328, 354, 1002
SsiI CCGC 2 cut(s) 365, 507
SspI AATATT 2 cut(s) 443, 844
SspMI CTAG 1 cut(s) 1062
StyD4I CCNGG 2 cut(s) 744, 889
TaaI ACNGT 3 cut(s) 204, 1015, 1028
TaiI ACGT 2 cut(s) 391, 574
TaqI TCGA 6 cut(s) 34, 102, 150, 447, 962, 981
TasI AATT 7 cut(s) 159, 182, 270, 289, 328, 354, 1002
TatI WGTACW 4 cut(s) 153, 204, 223, 300
TauI GCSGC 2 cut(s) 368, 510
TfiI GAWTC 4 cut(s) 47, 644, 650, 687
Tru1I TTAA 6 cut(s) 66, 333, 732, 828, 896, 1073
Tru9I TTAA 6 cut(s) 66, 333, 732, 828, 896, 1073
TscAI CASTG 1 cut(s) 1018
TspDTI ATGAA 6 cut(s) 279, 363, 532, 910, 1056, 1106
TspGWI ACGGA 2 cut(s) 40, 680
TspRI CASTG 1 cut(s) 1018
Van91I CCANNNNNTGG 1 cut(s) 241
Vha464I CTTAAG 1 cut(s) 731
XapI RAATTY 1 cut(s) 289
XmnI GAANNNNTTC 2 cut(s) 443, 468
XspI CTAG 1 cut(s) 1062
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.