Rroxscaffold_2G00080870

Protein of unknown function (DUF3754)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000718
Physical Location & Seq
Forward (+)
3796090 .. 3801650
5561 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00080870.1

Sequence Viewer

Length: 1437 bp
ATGGAAAAAACCGCGTCCCAGAAGAAGAAAGACATGATTCGAATAGAGCGCGAGTCCGTGATTCCGGTTCTCAAGCCCAAGCTCATCATGTCCTTGGCCAACCTCATTGAACACAGTTCCGACCGGGCGGAGTTTTTAAAGCTCTGCAAGAGAATTGAGTACACGATTCGGGCGTGGTATCTGCTACAATTCGAGGATTTGATGCAACTGTATTCGCTCTTTGACCCCGTCCATGGGAATCAAAAATTGGAGCAACAGAATCTTTCTCCTAAAGAGATTGATGTACTGGAACAGAATTTCCTCACCTACTTACTTCAGGTGATGGAAAAGAGTAATTTCAAGATTGCAACTGATGAAGAAATTGAGATTGCACATTCAGGGCAGTATCTTCTAAATCTTCCCATCGTGGTTGATGAATCTAAGATTGACAAGAAGCTTCTGAAGAACTTCTTTGCAGAGAATCCTCAGCAAAACCTTCCAGATTTTGCTGATAAGTACATTATCTTCCGGCGTGGTGTTGGACTTGATCAGACAACTGATTACTTTTTCATGGAGAAAGTGGACATGATCATTGCACGTTTTTGGGCATATCTTTTACGAATAACTAGGGTTGAAAAACTTTTATCCAGAAGGTCAAGTAGTCGGCACACAAAAGATCCAAAGAAGGATGATGAAATTACCTCAGAAGCAGATGGGGAGGAGGCGGAGGACTTATATGTTGAACGAATTCGTCTTGAAAATATGGAACTAAGCGTTAAAAGCTTGCTGAGCAAGACCACAATCAAAGAGCCAACCTTTGATAGGATAATTGTTGTTTACAGGCGAGCAAGTACCAAAGCAAAAGAAGAACGAGGAATATTTGTGAAACATTTCAAAAATATTCCCATGGCTGATATGGAGATAGTTCTTCCCGAGAAGAAGAACCCAGGATTAACTCCAATGGACTGGGTCAAGTTCCTTGGCTCTGCTGTAGTTGGGCTGGTTGCTGTTATTAGTTCGCTTGAAATGCCTAATGCTGACTTTTGGGTCATCATTGCAGTTCTTTCCACTGTCATTGGTTACTGTGCTAAGATATACTTCAGTTTTCAGCAGAACTTGGCTGCATATCAGAACCTAATAACACAGTCCATGTATGACAAGCAACTGGATAGCGGAAGGGGTACTCTTCTTCACTTGTGTGATGATGTGATTCAACAGGAGGTCAAGGAGGATCTGGATAGGTGGTGTGAGGAACTCATTAAAGAAGAGTTTGGTGCCAGTTGTAATTTTGATGTGGATGATGCAGTTGGGAAGTTGGAGAAATTGGGAATTGTCTCTCGGGATTCCGTTGGACGGTATTTCTGCGTTGGGCTGAAACGTGCAAATGAAATAATTGGGAGCACCACTGAGGAGCTTGTTATGAGGGCAAAACAAGGCAAACAAGGCAGTAGTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3754

Protein Analysis

478

Amino Acids

55.08

Weight (kDa)

5.59

Isoelectric Point (pI)

36.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3754 PF12576 266 - 387 6.8e-23 Protein of unknown function (DUF3754)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1025
AccB1I GGYRCC 1 cut(s) 1253
AccII CGCG 2 cut(s) 14, 51
AciI CCGC 4 cut(s) 12, 128, 704, 1152
AclWI GGATC 2 cut(s) 650, 1218
AcoI YGGCCR 1 cut(s) 96
AcsI RAATTY 2 cut(s) 295, 726
AcuI CTGAAG 3 cut(s) 299, 461, 1063
AfaI GTAC 5 cut(s) 161, 285, 497, 832, 1162
AfiI CCNNNNNNNGG 4 cut(s) 233, 234, 801, 1332
AgsI TTSAA 8 cut(s) 110, 340, 614, 722, 737, 874, 1004, 1193
AjnI CCWGG 1 cut(s) 925
AjuI GAANNNNNNNTTGG 2 cut(s) 230, 262
AleI CACNNNNGTG 1 cut(s) 1176
AloI GAACNNNNNNTCC 2 cut(s) 282, 314
AluBI AGCT 5 cut(s) 82, 142, 436, 762, 1393
AluI AGCT 5 cut(s) 82, 142, 436, 762, 1393
Alw21I GWGCWC 1 cut(s) 1382
Alw26I GTCTC 1 cut(s) 1318
AlwI GGATC 2 cut(s) 650, 1218
Ama87I CYCGRG 2 cut(s) 911, 1317
AoxI GGCC 1 cut(s) 96
ApeKI GCWGC 1 cut(s) 1100
ApoI RAATTY 2 cut(s) 295, 726
Asp700I GAANNNNTTC 3 cut(s) 446, 726, 869
AspLEI GCGC 1 cut(s) 51
AsuC2I CCSGG 1 cut(s) 125
AsuHPI GGTGA 2 cut(s) 295, 331
AsuII TTCGAA 1 cut(s) 40
AvaI CYCGRG 2 cut(s) 911, 1317
BalI TGGCCA 1 cut(s) 98
BanI GGYRCC 1 cut(s) 1253
BarI GAAGNNNNNNTAC 2 cut(s) 488, 520
Bbv12I GWGCWC 1 cut(s) 1382
BbvCI CCTCAGC 1 cut(s) 465
BbvI GCAGC 1 cut(s) 1087
BccI CCATC 3 cut(s) 316, 410, 686
BciT130I CCWGG 1 cut(s) 927
BclI TGATCA 2 cut(s) 526, 567
BcnI CCSGG 1 cut(s) 125
BcoDI GTCTC 1 cut(s) 1318
BfaI CTAG 1 cut(s) 606
BfmI CTRYAG 1 cut(s) 969
BisI GCNGC 1 cut(s) 1101
BlpI GCTNAGC 1 cut(s) 767
BlsI GCNGC 1 cut(s) 1102
Bme1390I CCNGG 2 cut(s) 125, 927
BmeT110I CYCGRG 2 cut(s) 911, 1317
BmiI GGNNCC 1 cut(s) 1255
BmrFI CCNGG 2 cut(s) 125, 927
BmrI ACTGGG 1 cut(s) 955
BmsI GCATC 2 cut(s) 192, 1270
BmuI ACTGGG 1 cut(s) 955
Bpu10I CCTNAGC 1 cut(s) 465
Bpu1102I GCTNAGC 1 cut(s) 767
Bpu14I TTCGAA 1 cut(s) 40
BpuEI CTTGAG 1 cut(s) 56
BpuMI CCSGG 1 cut(s) 125
BsaJI CCNNGG 5 cut(s) 93, 232, 885, 925, 958
BsaWI WCCGGW 1 cut(s) 64
Bsc4I CCNNNNNNNGG 4 cut(s) 233, 234, 801, 1332
Bse1I ACTGG 4 cut(s) 291, 950, 1149, 1257
Bse3DI GCAATG 2 cut(s) 570, 1032
BseBI CCWGG 1 cut(s) 927
BseDI CCNNGG 5 cut(s) 93, 232, 885, 925, 958
BseGI GGATG 2 cut(s) 673, 1282
BseLI CCNNNNNNNGG 4 cut(s) 233, 234, 801, 1332
BseMI GCAATG 2 cut(s) 570, 1032
BseMII CTCAG 4 cut(s) 479, 696, 758, 1377
BseNI ACTGG 4 cut(s) 291, 950, 1149, 1257
BseRI GAGGAG 2 cut(s) 713, 1403
BseXI GCAGC 1 cut(s) 1087
Bsh1236I CGCG 2 cut(s) 14, 51
Bsh1285I CGRYCG 1 cut(s) 124
BshFI GGCC 1 cut(s) 98
BshNI GGYRCC 1 cut(s) 1253
BsiEI CGRYCG 1 cut(s) 124
BsiHKAI GWGCWC 1 cut(s) 1382
BsiHKCI CYCGRG 2 cut(s) 911, 1317
BsiSI CCGG 3 cut(s) 65, 124, 508
BslI CCNNNNNNNGG 4 cut(s) 233, 234, 801, 1332
BsmAI GTCTC 1 cut(s) 1318
BsnI GGCC 1 cut(s) 98
BsoBI CYCGRG 2 cut(s) 911, 1317
Bsp119I TTCGAA 1 cut(s) 40
Bsp1286I GDGCHC 1 cut(s) 1382
Bsp143I GATC 4 cut(s) 526, 567, 655, 1210
Bsp1720I GCTNAGC 1 cut(s) 767
Bsp19I CCATGG 2 cut(s) 232, 885
BspACI CCGC 4 cut(s) 12, 128, 704, 1152
BspANI GGCC 1 cut(s) 98
BspCNI CTCAG 4 cut(s) 478, 695, 759, 1378
BspFNI CGCG 2 cut(s) 14, 51
BspLI GGNNCC 1 cut(s) 1255
BspPI GGATC 2 cut(s) 650, 1218
BspT104I TTCGAA 1 cut(s) 40
BspT107I GGYRCC 1 cut(s) 1253
BsrDI GCAATG 2 cut(s) 570, 1032
BsrI ACTGG 4 cut(s) 291, 950, 1149, 1257
BssECI CCNNGG 5 cut(s) 93, 232, 885, 925, 958
BssMI GATC 4 cut(s) 526, 567, 655, 1210
BssT1I CCWWGG 4 cut(s) 93, 232, 885, 958
Bst2UI CCWGG 1 cut(s) 927
Bst4CI ACNGT 6 cut(s) 116, 210, 1051, 1064, 1125, 1335
Bst6I CTCTTC 2 cut(s) 1170, 1239
BstBI TTCGAA 1 cut(s) 40
BstC8I GCNNGC 2 cut(s) 764, 825
BstDEI CTNAG 7 cut(s) 420, 465, 682, 749, 767, 1068, 1386
BstDSI CCRYGG 2 cut(s) 232, 885
BstENI CCTNNNNNAGG 1 cut(s) 799
BstF5I GGATG 2 cut(s) 673, 1282
BstFNI CGCG 2 cut(s) 14, 51
BstHHI GCGC 1 cut(s) 51
BstKTI GATC 4 cut(s) 529, 570, 658, 1213
BstMAI GTCTC 1 cut(s) 1318
BstMBI GATC 4 cut(s) 526, 567, 655, 1210
BstMCI CGRYCG 1 cut(s) 124
BstMWI GCNNNNNNNGC 4 cut(s) 759, 768, 1006, 1422
BstNI CCWGG 1 cut(s) 927
BstSCI CCNGG 2 cut(s) 123, 925
BstSFI CTRYAG 1 cut(s) 969
BstUI CGCG 2 cut(s) 14, 51
BstV1I GCAGC 1 cut(s) 1087
BstX2I RGATCY 2 cut(s) 655, 1210
BstXI CCANNNNNNTGG 1 cut(s) 945
BstYI RGATCY 2 cut(s) 655, 1210
BsuRI GGCC 1 cut(s) 98
BtgI CCRYGG 2 cut(s) 232, 885
BtsCI GGATG 2 cut(s) 673, 1282
BtsIMutI CAGTG 2 cut(s) 1047, 1383
Cac8I GCNNGC 2 cut(s) 764, 825
CfoI GCGC 1 cut(s) 51
CseI GACGC 1 cut(s) 3
Csp6I GTAC 5 cut(s) 160, 284, 496, 831, 1161
CspCI CAANNNNNGTGG 2 cut(s) 1036, 1071
CviAII CATG 7 cut(s) 34, 88, 233, 550, 565, 886, 1129
CviQI GTAC 5 cut(s) 160, 284, 496, 831, 1161
DdeI CTNAG 7 cut(s) 420, 465, 682, 749, 767, 1068, 1386
DpnI GATC 4 cut(s) 528, 569, 657, 1212
DpnII GATC 4 cut(s) 526, 567, 655, 1210
DraI TTTAAA 1 cut(s) 138
DrdI GACNNNNNNGTC 1 cut(s) 1025
DseDI GACNNNNNNGTC 1 cut(s) 1025
EaeI YGGCCR 1 cut(s) 96
Eam1104I CTCTTC 2 cut(s) 1170, 1239
EarI CTCTTC 2 cut(s) 1170, 1239
EciI GGCGGA 2 cut(s) 143, 719
Eco130I CCWWGG 4 cut(s) 93, 232, 885, 958
Eco57I CTGAAG 3 cut(s) 299, 461, 1063
Eco88I CYCGRG 2 cut(s) 911, 1317
EcoNI CCTNNNNNAGG 1 cut(s) 799
EcoRI GAATTC 1 cut(s) 726
EcoRII CCWGG 1 cut(s) 925
EcoT14I CCWWGG 4 cut(s) 93, 232, 885, 958
ErhI CCWWGG 4 cut(s) 93, 232, 885, 958
FaeI CATG 7 cut(s) 37, 91, 236, 553, 568, 889, 1132
FalI AAGNNNNNCTT 4 cut(s) 434, 466, 1061, 1093
FatI CATG 7 cut(s) 33, 87, 232, 549, 564, 885, 1128
FbaI TGATCA 2 cut(s) 526, 567
Fnu4HI GCNGC 1 cut(s) 1101
FokI GGATG 2 cut(s) 680, 1289
Fsp4HI GCNGC 1 cut(s) 1101
FspBI CTAG 1 cut(s) 606
GlaI GCGC 1 cut(s) 50
GluI GCNGC 1 cut(s) 1101
HaeIII GGCC 1 cut(s) 98
HapII CCGG 3 cut(s) 65, 124, 508
HgaI GACGC 1 cut(s) 3
HhaI GCGC 1 cut(s) 51
Hin1II CATG 7 cut(s) 37, 91, 236, 553, 568, 889, 1132
Hin6I GCGC 1 cut(s) 49
HinP1I GCGC 1 cut(s) 49
HindIII AAGCTT 2 cut(s) 434, 760
HpaII CCGG 3 cut(s) 65, 124, 508
HphI GGTGA 2 cut(s) 295, 331
Hpy166II GTNNAC 3 cut(s) 162, 562, 817
Hpy188I TCNGA 5 cut(s) 121, 441, 531, 685, 1110
Hpy188III TCNNGA 8 cut(s) 340, 479, 627, 734, 911, 1214, 1319, 1434
Hpy8I GTNNAC 3 cut(s) 162, 562, 817
HpyAV CCTTC 4 cut(s) 485, 624, 658, 1149
HpyCH4III ACNGT 6 cut(s) 116, 210, 1051, 1064, 1125, 1335
HpyCH4IV ACGT 2 cut(s) 577, 1357
HpyF10VI GCNNNNNNNGC 4 cut(s) 759, 768, 1006, 1422
HpyF3I CTNAG 7 cut(s) 420, 465, 682, 749, 767, 1068, 1386
HpySE526I ACGT 2 cut(s) 577, 1357
Hsp92II CATG 7 cut(s) 37, 91, 236, 553, 568, 889, 1132
HspAI GCGC 1 cut(s) 49
Ksp22I TGATCA 2 cut(s) 526, 567
Kzo9I GATC 4 cut(s) 526, 567, 655, 1210
LmnI GCTCC 3 cut(s) 250, 1377, 1390
Lsp1109I GCAGC 1 cut(s) 1087
LweI GCATC 2 cut(s) 192, 1270
MaeI CTAG 1 cut(s) 606
MaeII ACGT 2 cut(s) 577, 1357
MaeIII GTNAC 1 cut(s) 1058
MalI GATC 4 cut(s) 528, 569, 657, 1212
MboI GATC 4 cut(s) 526, 567, 655, 1210
MflI RGATCY 2 cut(s) 655, 1210
MhlI GDGCHC 1 cut(s) 1382
MlsI TGGCCA 1 cut(s) 98
MluNI TGGCCA 1 cut(s) 98
MlyI GAGTC 1 cut(s) 62
MmeI TCCRAC 4 cut(s) 144, 499, 1275, 1309
Mox20I TGGCCA 1 cut(s) 98
MroXI GAANNNNTTC 3 cut(s) 446, 726, 869
MscI TGGCCA 1 cut(s) 98
MseI TTAA 4 cut(s) 137, 756, 932, 1239
MslI CAYNNNNRTG 1 cut(s) 1176
Msp20I TGGCCA 1 cut(s) 98
MspI CCGG 3 cut(s) 65, 124, 508
MspR9I CCNGG 2 cut(s) 125, 927
MvaI CCWGG 1 cut(s) 927
MvnI CGCG 2 cut(s) 14, 51
MwoI GCNNNNNNNGC 4 cut(s) 759, 768, 1006, 1422
NciI CCSGG 1 cut(s) 125
NcoI CCATGG 2 cut(s) 232, 885
NdeII GATC 4 cut(s) 526, 567, 655, 1210
NlaIII CATG 7 cut(s) 37, 91, 236, 553, 568, 889, 1132
NlaIV GGNNCC 1 cut(s) 1255
NspV TTCGAA 1 cut(s) 40
OliI CACNNNNGTG 1 cut(s) 1176
PcsI WCGNNNNNNNCGW 1 cut(s) 170
PdmI GAANNNNTTC 3 cut(s) 446, 726, 869
PfeI GAWTC 9 cut(s) 37, 61, 166, 238, 259, 416, 460, 1189, 1322
PflFI GACNNNGTC 2 cut(s) 227, 947
PkrI GCNGC 1 cut(s) 1102
PleI GAGTC 1 cut(s) 61
PpsI GAGTC 1 cut(s) 61
Psp6I CCWGG 1 cut(s) 925
PspGI CCWGG 1 cut(s) 925
PspN4I GGNNCC 1 cut(s) 1255
PsuI RGATCY 2 cut(s) 655, 1210
PsyI GACNNNGTC 2 cut(s) 227, 947
RsaI GTAC 5 cut(s) 161, 285, 497, 832, 1162
RsaNI GTAC 5 cut(s) 160, 284, 496, 831, 1161
RseI CAYNNNNRTG 1 cut(s) 1176
SaqAI TTAA 4 cut(s) 137, 756, 932, 1239
SatI GCNGC 1 cut(s) 1101
Sau3AI GATC 4 cut(s) 526, 567, 655, 1210
SchI GAGTC 1 cut(s) 62
ScrFI CCNGG 2 cut(s) 125, 927
SduI GDGCHC 1 cut(s) 1382
SfaNI GCATC 2 cut(s) 192, 1270
SfcI CTRYAG 1 cut(s) 969
SfuI TTCGAA 1 cut(s) 40
SmiMI CAYNNNNRTG 1 cut(s) 1176
SmlI CTYRAG 1 cut(s) 71
SmoI CTYRAG 1 cut(s) 71
SsiI CCGC 4 cut(s) 12, 128, 704, 1152
SspI AATATT 2 cut(s) 858, 880
SspMI CTAG 1 cut(s) 606
StyD4I CCNGG 2 cut(s) 123, 925
StyI CCWWGG 4 cut(s) 93, 232, 885, 958
TaaI ACNGT 6 cut(s) 116, 210, 1051, 1064, 1125, 1335
TaiI ACGT 2 cut(s) 580, 1360
TaqI TCGA 2 cut(s) 40, 192
TatI WGTACW 3 cut(s) 159, 283, 495
TfiI GAWTC 9 cut(s) 37, 61, 166, 238, 259, 416, 460, 1189, 1322
Tru1I TTAA 4 cut(s) 137, 756, 932, 1239
Tru9I TTAA 4 cut(s) 137, 756, 932, 1239
TscAI CASTG 2 cut(s) 1054, 1390
TseI GCWGC 1 cut(s) 1100
TspDTI ATGAA 5 cut(s) 369, 429, 538, 687, 1380
TspGWI ACGGA 2 cut(s) 46, 1315
TspRI CASTG 2 cut(s) 1054, 1390
Tth111I GACNNNGTC 2 cut(s) 227, 947
XagI CCTNNNNNAGG 1 cut(s) 799
XapI RAATTY 2 cut(s) 295, 726
XcmI CCANNNNNNNNNTGG 1 cut(s) 892
XmnI GAANNNNTTC 3 cut(s) 446, 726, 869
XspI CTAG 1 cut(s) 606
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.