Prupe.3G282800_v2.0.a1

Protein of unknown function (DUF3754)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0000718
Physical Location & Seq
Reverse (-)
25712903 .. 25717833
4931 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G282800.1

Sequence Viewer

Length: 1464 bp
ATGGAGCAAAAGAAGAAGGACATGATTCGAATTGAGCGTGAATCTGTCATCCCAGTTATCAAGCCCAAAATCATCATGACCTTGGCCAACCTCATTGAACATAGTTCTGACCGGGCTGAGTTTTTAAAGCTCTGCAAGAGAGTTGAGTACACAATTCGGGCTTGGTATCTTCTACAGTTCGAGGATTTGATGCAACTTTACTCCCTCTTTGACCCTGTAAATGGGGCTAAAAAATTGGAACAACACAATCTTCCTCCTAAAGAAATTGATGTACTTGAACAGAATTTCCTCACGTACTTATTTCAGGTGATGGAAAAGAGTAATTTCAAAATAGCAAGCAATGAGGAAATTGAGGTTGCGCATTCAGGGCAGTATCTTCTAAATCTACCAATCGTGGTTGATGAGTCTAAGCTTGACAAGAAGCTTTTAAAGAAATTTTTTGAAAGGCGTCCTCAACCAAACCTTCCCGATTTTTGTGATAAGTACATTATCTTCCGACGCGGTGTTGGACTTGATCAGACAACTGATTACTTTTTCATGGAGAAAGTGGACATGATCATTGCACGCTTTTGGGCTTATCTTTTACGAATAACTAAGGTCGGAAAACTTTTATCCAGAAGATCAAGTGGACGACCTAAGACAGATCCAAAGAAGAATGAGGAAATTAGCTCTGAAGCAGATGGAGAGGATGAAGACTTACATGTTGAACGAATCCGTCTAGAAAAAATGGAACTAAGTGTTAAAAATTTGCTGAGCAAGACCACGATCAAAGAGCCAACCTTTGATAGGATAATTGTTGTTTACAGGCGAGCAAGTACCAAAGCAAAAGAGGAACGAGGAATATATGTGAAACATTTCAGAAATATTCCGATGGCTGATATGGAGATAGTTCTTCCTGAGAAGAAGAACCCTGGGTTAACCCCAATGGACTGGGTCAAGTTCCTTGGCTCTGCTATAGTTGGGCTGGTTGCTGTTGTTGGTTCGATTGAAATGCCTAAAGCTGATCTTTGGGTCATTTTTGCAGTTCTTTCCACTGTGATTGGTTACTGTGCTAAGATATACTTCACTTTTCAGCAGAACTTGGTAGCATATCAGAACTTAATAACACAGTCCATGTACGACAAACAACTCGATAGCGGAAGGGGTACTCTTCTTCACTTGTGTGATGATGTGATTCAACAGGAAGTCAAGGAGGTGATAATTGCTTTCTATATACTAATGGAGCAAGGGAAAGCTACAACACAAGATCTGGATAGGTGGTGTGAGGAACTAATTAAAGAAGAGTTTAACGCCAACTGTAATTTTGATGTGGAAGATGCAGTTCATAAGTTGATGAAATTAGGAATTGTTGCTCAGGATTCCGTTGGACGGTTCTACTGCGTTGGGCTGAAACGTGCTAATGAGATCATCGGCACCACCACTGAGGAGCTTGTCCTTAAGGCAAAACAAGGTGGGAGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3754

Protein Analysis

488

Amino Acids

56.28

Weight (kDa)

6.71

Isoelectric Point (pI)

39.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 360
AccB1I GGYRCC 1 cut(s) 1412
AccII CGCG 1 cut(s) 501
AciI CCGC 2 cut(s) 501, 1137
AclWI GGATC 1 cut(s) 638
AcoI YGGCCR 1 cut(s) 84
AcsI RAATTY 3 cut(s) 283, 434, 745
AcuI CTGAAG 1 cut(s) 693
AcyI GRCGYC 1 cut(s) 448
AfaI GTAC 7 cut(s) 149, 273, 296, 485, 817, 1118, 1147
AfiI CCNNNNNNNGG 3 cut(s) 221, 786, 1368
AflII CTTAAG 1 cut(s) 1436
AflIII ACRYGT 1 cut(s) 700
AgsI TTSAA 7 cut(s) 98, 278, 328, 443, 707, 989, 1178
AjnI CCWGG 1 cut(s) 910
AleI CACNNNNGTG 1 cut(s) 1161
AloI GAACNNNNNNTCC 2 cut(s) 270, 302
AluBI AGCT 7 cut(s) 130, 412, 424, 669, 1001, 1235, 1429
AluI AGCT 7 cut(s) 130, 412, 424, 669, 1001, 1235, 1429
AlwI GGATC 1 cut(s) 638
AoxI GGCC 1 cut(s) 84
ApoI RAATTY 3 cut(s) 283, 434, 745
Asp700I GAANNNNTTC 1 cut(s) 854
AspLEI GCGC 1 cut(s) 361
AsuC2I CCSGG 1 cut(s) 113
AsuHPI GGTGA 2 cut(s) 319, 1207
AsuII TTCGAA 1 cut(s) 28
BalI TGGCCA 1 cut(s) 86
BanI GGYRCC 1 cut(s) 1412
BarI GAAGNNNNNNTAC 2 cut(s) 476, 508
BbsI GAAGAC 1 cut(s) 699
BccI CCATC 3 cut(s) 304, 674, 865
BcgI CGANNNNNNTGC 2 cut(s) 973, 1007
BciT130I CCWGG 1 cut(s) 912
BclI TGATCA 2 cut(s) 514, 555
BcnI CCSGG 1 cut(s) 113
BfaI CTAG 1 cut(s) 719
BfmI CTRYAG 2 cut(s) 173, 954
BfrI CTTAAG 1 cut(s) 1436
BglII AGATCT 1 cut(s) 1246
BlpI GCTNAGC 1 cut(s) 752
Bme1390I CCNGG 2 cut(s) 113, 912
BmiI GGNNCC 1 cut(s) 1414
BmrFI CCNGG 2 cut(s) 113, 912
BmrI ACTGGG 2 cut(s) 47, 940
BmsI GCATC 2 cut(s) 180, 1306
BmuI ACTGGG 2 cut(s) 47, 940
BpiI GAAGAC 1 cut(s) 699
Bpu10I CCTNAGC 1 cut(s) 1353
Bpu1102I GCTNAGC 1 cut(s) 752
Bpu14I TTCGAA 1 cut(s) 28
BpuMI CCSGG 1 cut(s) 113
BsaAI YACGTR 1 cut(s) 294
BsaHI GRCGYC 1 cut(s) 448
BsaJI CCNNGG 4 cut(s) 81, 910, 911, 943
Bsc4I CCNNNNNNNGG 3 cut(s) 221, 786, 1368
Bse1I ACTGG 2 cut(s) 53, 935
Bse3DI GCAATG 2 cut(s) 346, 558
BseBI CCWGG 1 cut(s) 912
BseDI CCNNGG 4 cut(s) 81, 910, 911, 943
BseGI GGATG 2 cut(s) 48, 694
BseLI CCNNNNNNNGG 3 cut(s) 221, 786, 1368
BseMI GCAATG 2 cut(s) 346, 558
BseMII CTCAG 5 cut(s) 108, 743, 888, 1367, 1413
BseNI ACTGG 2 cut(s) 53, 935
BseRI GAGGAG 1 cut(s) 1439
Bsh1236I CGCG 1 cut(s) 501
BshFI GGCC 1 cut(s) 86
BshNI GGYRCC 1 cut(s) 1412
BsiSI CCGG 1 cut(s) 112
BslI CCNNNNNNNGG 3 cut(s) 221, 786, 1368
BsmI GAATGC 1 cut(s) 361
BsnI GGCC 1 cut(s) 86
Bsp119I TTCGAA 1 cut(s) 28
Bsp143I GATC 8 cut(s) 514, 555, 620, 643, 765, 1003, 1246, 1404
Bsp1720I GCTNAGC 1 cut(s) 752
BspACI CCGC 2 cut(s) 501, 1137
BspANI GGCC 1 cut(s) 86
BspCNI CTCAG 5 cut(s) 109, 744, 889, 1366, 1414
BspFNI CGCG 1 cut(s) 501
BspHI TCATGA 1 cut(s) 75
BspLI GGNNCC 1 cut(s) 1414
BspPI GGATC 1 cut(s) 638
BspT104I TTCGAA 1 cut(s) 28
BspT107I GGYRCC 1 cut(s) 1412
BspTI CTTAAG 1 cut(s) 1436
BsrDI GCAATG 2 cut(s) 346, 558
BsrI ACTGG 2 cut(s) 53, 935
BssECI CCNNGG 4 cut(s) 81, 910, 911, 943
BssMI GATC 8 cut(s) 514, 555, 620, 643, 765, 1003, 1246, 1404
BssNI GRCGYC 1 cut(s) 448
BssT1I CCWWGG 2 cut(s) 81, 943
Bst2UI CCWGG 1 cut(s) 912
Bst4CI ACNGT 6 cut(s) 177, 1036, 1049, 1110, 1298, 1371
Bst6I CTCTTC 2 cut(s) 1155, 1275
BstACI GRCGYC 1 cut(s) 448
BstAFI CTTAAG 1 cut(s) 1436
BstBAI YACGTR 1 cut(s) 294
BstBI TTCGAA 1 cut(s) 28
BstC8I GCNNGC 3 cut(s) 337, 565, 810
BstENI CCTNNNNNAGG 1 cut(s) 784
BstF5I GGATG 2 cut(s) 48, 694
BstFNI CGCG 1 cut(s) 501
BstHHI GCGC 1 cut(s) 361
BstKTI GATC 8 cut(s) 517, 558, 623, 646, 768, 1006, 1249, 1407
BstMBI GATC 8 cut(s) 514, 555, 620, 643, 765, 1003, 1246, 1404
BstMWI GCNNNNNNNGC 1 cut(s) 367
BstNI CCWGG 1 cut(s) 912
BstNSI RCATGY 1 cut(s) 704
BstSCI CCNGG 2 cut(s) 111, 910
BstSFI CTRYAG 2 cut(s) 173, 954
BstUI CGCG 1 cut(s) 501
BstV2I GAAGAC 1 cut(s) 699
BstX2I RGATCY 2 cut(s) 643, 1246
BstXI CCANNNNNNTGG 1 cut(s) 930
BstYI RGATCY 2 cut(s) 643, 1246
BsuRI GGCC 1 cut(s) 86
BtsCI GGATG 2 cut(s) 48, 694
BtsIMutI CAGTG 2 cut(s) 1032, 1419
Cac8I GCNNGC 3 cut(s) 337, 565, 810
CciI TCATGA 1 cut(s) 75
CfoI GCGC 1 cut(s) 361
CseI GACGC 2 cut(s) 437, 507
Csp6I GTAC 7 cut(s) 148, 272, 295, 484, 816, 1117, 1146
CspCI CAANNNNNGTGG 2 cut(s) 1021, 1056
CviAII CATG 6 cut(s) 22, 76, 538, 553, 701, 1114
CviQI GTAC 7 cut(s) 148, 272, 295, 484, 816, 1117, 1146
DpnI GATC 8 cut(s) 516, 557, 622, 645, 767, 1005, 1248, 1406
DpnII GATC 8 cut(s) 514, 555, 620, 643, 765, 1003, 1246, 1404
DraI TTTAAA 2 cut(s) 126, 429
EaeI YGGCCR 1 cut(s) 84
Eam1104I CTCTTC 2 cut(s) 1155, 1275
EarI CTCTTC 2 cut(s) 1155, 1275
Eco130I CCWWGG 2 cut(s) 81, 943
Eco57I CTGAAG 1 cut(s) 693
EcoNI CCTNNNNNAGG 1 cut(s) 784
EcoRII CCWGG 1 cut(s) 910
EcoT14I CCWWGG 2 cut(s) 81, 943
ErhI CCWWGG 2 cut(s) 81, 943
FaeI CATG 6 cut(s) 25, 79, 541, 556, 704, 1117
FalI AAGNNNNNCTT 4 cut(s) 990, 1022, 1046, 1078
FatI CATG 6 cut(s) 21, 75, 537, 552, 700, 1113
FbaI TGATCA 2 cut(s) 514, 555
FokI GGATG 2 cut(s) 35, 701
FspBI CTAG 1 cut(s) 719
FspI TGCGCA 1 cut(s) 360
GlaI GCGC 1 cut(s) 360
HaeIII GGCC 1 cut(s) 86
HapII CCGG 1 cut(s) 112
HgaI GACGC 2 cut(s) 437, 507
HhaI GCGC 1 cut(s) 361
Hin1I GRCGYC 1 cut(s) 448
Hin1II CATG 6 cut(s) 25, 79, 541, 556, 704, 1117
Hin6I GCGC 1 cut(s) 359
HinP1I GCGC 1 cut(s) 359
HincII GTYRAC 1 cut(s) 918
HindII GTYRAC 1 cut(s) 918
HindIII AAGCTT 2 cut(s) 410, 422
HinfI GANTC 6 cut(s) 25, 41, 404, 711, 1174, 1358
HpaI GTTAAC 1 cut(s) 918
HpaII CCGG 1 cut(s) 112
HphI GGTGA 2 cut(s) 319, 1207
Hpy166II GTNNAC 5 cut(s) 150, 550, 629, 802, 918
Hpy188I TCNGA 8 cut(s) 109, 497, 519, 602, 673, 860, 870, 1095
Hpy188III TCNNGA 7 cut(s) 76, 467, 615, 719, 896, 1250, 1355
Hpy8I GTNNAC 5 cut(s) 150, 550, 629, 802, 918
Hpy99I CGWCG 1 cut(s) 501
HpyAV CCTTC 3 cut(s) 10, 473, 1134
HpyCH4III ACNGT 6 cut(s) 177, 1036, 1049, 1110, 1298, 1371
HpyCH4IV ACGT 2 cut(s) 293, 1393
HpyCH4V TGCA 5 cut(s) 135, 193, 563, 1022, 1319
HpyF10VI GCNNNNNNNGC 1 cut(s) 367
HpySE526I ACGT 2 cut(s) 293, 1393
Hsp92I GRCGYC 1 cut(s) 448
Hsp92II CATG 6 cut(s) 25, 79, 541, 556, 704, 1117
HspAI GCGC 1 cut(s) 359
Ksp22I TGATCA 2 cut(s) 514, 555
KspAI GTTAAC 1 cut(s) 918
Kzo9I GATC 8 cut(s) 514, 555, 620, 643, 765, 1003, 1246, 1404
LmnI GCTCC 3 cut(s) 4, 1222, 1426
LweI GCATC 2 cut(s) 180, 1306
MaeI CTAG 1 cut(s) 719
MaeII ACGT 2 cut(s) 293, 1393
MaeIII GTNAC 1 cut(s) 1043
MalI GATC 8 cut(s) 516, 557, 622, 645, 767, 1005, 1248, 1406
MboI GATC 8 cut(s) 514, 555, 620, 643, 765, 1003, 1246, 1404
MflI RGATCY 2 cut(s) 643, 1246
MlsI TGGCCA 1 cut(s) 86
MluNI TGGCCA 1 cut(s) 86
MlyI GAGTC 1 cut(s) 413
MmeI TCCRAC 4 cut(s) 487, 520, 580, 1345
Mox20I TGGCCA 1 cut(s) 86
MroXI GAANNNNTTC 1 cut(s) 854
MscI TGGCCA 1 cut(s) 86
MseI TTAA 8 cut(s) 125, 428, 741, 917, 1100, 1275, 1287, 1437
MslI CAYNNNNRTG 1 cut(s) 1161
Msp20I TGGCCA 1 cut(s) 86
MspCI CTTAAG 1 cut(s) 1436
MspI CCGG 1 cut(s) 112
MspR9I CCNGG 2 cut(s) 113, 912
Mva1269I GAATGC 1 cut(s) 361
MvaI CCWGG 1 cut(s) 912
MvnI CGCG 1 cut(s) 501
MwoI GCNNNNNNNGC 1 cut(s) 367
NciI CCSGG 1 cut(s) 113
NdeII GATC 8 cut(s) 514, 555, 620, 643, 765, 1003, 1246, 1404
NlaIII CATG 6 cut(s) 25, 79, 541, 556, 704, 1117
NlaIV GGNNCC 1 cut(s) 1414
NsbI TGCGCA 1 cut(s) 360
NspI RCATGY 1 cut(s) 704
NspV TTCGAA 1 cut(s) 28
OliI CACNNNNGTG 1 cut(s) 1161
PagI TCATGA 1 cut(s) 75
PasI CCCWGGG 1 cut(s) 911
PciI ACATGT 1 cut(s) 700
PcsI WCGNNNNNNNCGW 1 cut(s) 34
PctI GAATGC 1 cut(s) 361
PdmI GAANNNNTTC 1 cut(s) 854
PfeI GAWTC 5 cut(s) 25, 41, 711, 1174, 1358
PflFI GACNNNGTC 1 cut(s) 932
PleI GAGTC 1 cut(s) 412
PpsI GAGTC 1 cut(s) 412
Ppu21I YACGTR 1 cut(s) 294
PscI ACATGT 1 cut(s) 700
Psp6I CCWGG 1 cut(s) 910
PspGI CCWGG 1 cut(s) 910
PspN4I GGNNCC 1 cut(s) 1414
PsuI RGATCY 2 cut(s) 643, 1246
PsyI GACNNNGTC 1 cut(s) 932
RsaI GTAC 7 cut(s) 149, 273, 296, 485, 817, 1118, 1147
RsaNI GTAC 7 cut(s) 148, 272, 295, 484, 816, 1117, 1146
RseI CAYNNNNRTG 1 cut(s) 1161
SaqAI TTAA 8 cut(s) 125, 428, 741, 917, 1100, 1275, 1287, 1437
Sau3AI GATC 8 cut(s) 514, 555, 620, 643, 765, 1003, 1246, 1404
SchI GAGTC 1 cut(s) 413
ScrFI CCNGG 2 cut(s) 113, 912
SfaNI GCATC 2 cut(s) 180, 1306
SfcI CTRYAG 2 cut(s) 173, 954
SfuI TTCGAA 1 cut(s) 28
SmiMI CAYNNNNRTG 1 cut(s) 1161
SmlI CTYRAG 1 cut(s) 1436
SmoI CTYRAG 1 cut(s) 1436
SsiI CCGC 2 cut(s) 501, 1137
SspI AATATT 1 cut(s) 865
SspMI CTAG 1 cut(s) 719
StyD4I CCNGG 2 cut(s) 111, 910
StyI CCWWGG 2 cut(s) 81, 943
TaaI ACNGT 6 cut(s) 177, 1036, 1049, 1110, 1298, 1371
TaiI ACGT 2 cut(s) 296, 1396
TaqI TCGA 4 cut(s) 28, 180, 983, 1131
TatI WGTACW 3 cut(s) 147, 271, 483
TfiI GAWTC 5 cut(s) 25, 41, 711, 1174, 1358
Tru1I TTAA 8 cut(s) 125, 428, 741, 917, 1100, 1275, 1287, 1437
Tru9I TTAA 8 cut(s) 125, 428, 741, 917, 1100, 1275, 1287, 1437
TscAI CASTG 2 cut(s) 1039, 1426
TspDTI ATGAA 4 cut(s) 526, 705, 1313, 1349
TspGWI ACGGA 2 cut(s) 704, 1351
TspRI CASTG 2 cut(s) 1039, 1426
Tth111I GACNNNGTC 1 cut(s) 932
Vha464I CTTAAG 1 cut(s) 1436
XagI CCTNNNNNAGG 1 cut(s) 784
XapI RAATTY 3 cut(s) 283, 434, 745
XbaI TCTAGA 1 cut(s) 718
XceI RCATGY 1 cut(s) 704
XmnI GAANNNNTTC 1 cut(s) 854
XspI CTAG 1 cut(s) 719
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.