Rh2DG699400

Protein of unknown function (DUF3754)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000718
Physical Location & Seq
Forward (+)
91436384 .. 91440297
3914 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG699400.1

Sequence Viewer

Length: 1275 bp
ATGGAAAAGAGTAATTTTAAGATTGCAAGTAATGAAGAAATTGACATTGCGACTTCAGGGGAGTATCTTCTACATCTTCCCATTGTGGTTGATGAGTCTAAGCTTGACAACAAACTTTTGAAGAAATATTTTGAAAATAATCCTGCCGAAGACATTCCAGATTTTTCTGATAAGTATGTCATCTTCCGACGCGGCATTGGACTAGATAAGAAAACCGATTTCTTTATTATGGAGAAGGTGGACCTGATCATTTCGCGTTTTTGGAAATATCTTTTACGAATAACTACGTTAGACAAATTTTTCAAGAAACCAGCCCCTAAGAAGAGGGATTCAGAATCTGATACAGATGAGGATGACTTCTTTGTTGAACGAATCCGTCTTGAAAACATGGAACTAAGCATTCAAAATTTAATTCTACCGAACACAATCCAGGAGCCAACTTTTGATAGGATTATCGTTGTTTACAGGCAAGCAACTACTGATGAGGATAGTCCAGAACGGGCTATACATATTAAACATTTCAAAAATATTCCAATGGCTGATTTGGAGATAGTTCTTCCTGAAAAGAAGAGCCCAGGTTTAACACCAATGGACTGGCTCTCGTTCATTGGCTCTGCTGTAGTTGGTCTGATTGCTGTTTTTACTTCAATTGAAACGACTAAAGTCGATCTTTGGGTTATATTTGCAATTCTTTCCACATTGATTGGTTACTGTGCTAAGACATATTTCAGTTTTCAGCAGAACCTAGCCGATTATCAGAACTTGATAACACAGTCCATGTACGAAAAACAACTAGATAGCGGAAGGGGAACTCTTCTTCACTTGTGTGATGATGTGATTCAACAGGAAGTCAAGGAAGTAATAATTTCTTTCCTTGTGTTAAATGAAAGAGGGAATTCTACTTTACCAGATCTGGACAAGACGTGTGAGTGGATGATGAAAACTTGGTTTAACGACGAGATCAATTTTGATGTGGACGATGCAGTTAAGAAGCTAGAGAAATTGGGAATTGTTACTAAGGATAGCGAGGGACGGTATAGCTCTGTTGGGCTGAAACGTGCTAACGAGATCATTGGCACCACCACTGAGGAGCTTGTCCTTAAGGCAAAAGAAGATGCAGCTGCTAATGCTAATACTCCTGCTTCTCCTGCAGCAACGACTCCTGCAGTCGGCGCTGACAGCGGCGGCAGCGACAACGCCGACGATGATAACTATGCTGCCGACACCGATGAGGATAAATATGCTGCCGACACCGATGTACTCCTAAATAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3754

Protein Analysis

424

Amino Acids

47.98

Weight (kDa)

4.62

Isoelectric Point (pI)

30.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3754 PF12576 148 - 270 3.1e-24 Protein of unknown function (DUF3754)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1076
AccII CGCG 2 cut(s) 192, 256
AciI CCGC 4 cut(s) 192, 801, 1182, 1185
AcsI RAATTY 3 cut(s) 296, 406, 895
AcuI CTGAAG 1 cut(s) 39
AfaI GTAC 2 cut(s) 782, 1260
AfiI CCNNNNNNNGG 1 cut(s) 1169
AflII CTTAAG 1 cut(s) 1100
AflIII ACRYGT 1 cut(s) 923
AjiI CACGTC 1 cut(s) 924
AjnI CCWGG 2 cut(s) 429, 574
AleI CACNNNNGTG 1 cut(s) 825
AluBI AGCT 5 cut(s) 103, 994, 1041, 1093, 1121
AluI AGCT 5 cut(s) 103, 994, 1041, 1093, 1121
AlwNI CAGNNNCTG 1 cut(s) 338
ApeKI GCWGC 6 cut(s) 1118, 1121, 1151, 1188, 1217, 1244
ApoI RAATTY 3 cut(s) 296, 406, 895
Asp700I GAANNNNTTC 1 cut(s) 153
AspLEI GCGC 1 cut(s) 1175
AspS9I GGNCC 1 cut(s) 241
AvaII GGWCC 1 cut(s) 241
BanI GGYRCC 1 cut(s) 1076
BanII GRGCYC 1 cut(s) 575
BarI GAAGNNNNNNTAC 2 cut(s) 167, 199
BbsI GAAGAC 1 cut(s) 156
BbvI GCAGC 6 cut(s) 1108, 1130, 1163, 1200, 1204, 1231
BciT130I CCWGG 2 cut(s) 431, 576
BclI TGATCA 1 cut(s) 246
BfaI CTAG 4 cut(s) 203, 746, 794, 995
BfmI CTRYAG 3 cut(s) 618, 1149, 1164
BfoI RGCGCY 1 cut(s) 1176
BfrI CTTAAG 1 cut(s) 1100
BglII AGATCT 1 cut(s) 910
BisI GCNGC 9 cut(s) 193, 1119, 1122, 1152, 1183, 1186, 1189, 1218, 1245
BlsI GCNGC 9 cut(s) 194, 1120, 1123, 1153, 1184, 1187, 1190, 1219, 1246
Bme1390I CCNGG 2 cut(s) 431, 576
Bme18I GGWCC 1 cut(s) 241
BmgBI CACGTC 1 cut(s) 924
BmgT120I GGNCC 1 cut(s) 241
BmiI GGNNCC 2 cut(s) 435, 1078
BmrFI CCNGG 2 cut(s) 431, 576
BmsI GCATC 2 cut(s) 970, 1105
BoxI GACNNNNGTC 1 cut(s) 662
BpiI GAAGAC 1 cut(s) 156
BsaJI CCNNGG 1 cut(s) 574
Bsc4I CCNNNNNNNGG 1 cut(s) 1169
Bse1I ACTGG 1 cut(s) 599
Bse3DI GCAATG 1 cut(s) 45
BseBI CCWGG 2 cut(s) 431, 576
BseDI CCNNGG 1 cut(s) 574
BseGI GGATG 2 cut(s) 358, 939
BseLI CCNNNNNNNGG 1 cut(s) 1169
BseMI GCAATG 1 cut(s) 45
BseMII CTCAG 1 cut(s) 1077
BseNI ACTGG 1 cut(s) 599
BseRI GAGGAG 1 cut(s) 1103
BseXI GCAGC 6 cut(s) 1108, 1130, 1163, 1200, 1204, 1231
Bsh1236I CGCG 2 cut(s) 192, 256
BshNI GGYRCC 1 cut(s) 1076
BslFI GGGAC 1 cut(s) 1044
BslI CCNNNNNNNGG 1 cut(s) 1169
BsmFI GGGAC 1 cut(s) 1044
BsmI GAATGC 1 cut(s) 399
Bsp1286I GDGCHC 1 cut(s) 575
Bsp143I GATC 5 cut(s) 246, 667, 910, 960, 1068
BspACI CCGC 4 cut(s) 192, 801, 1182, 1185
BspCNI CTCAG 1 cut(s) 1078
BspFNI CGCG 2 cut(s) 192, 256
BspLI GGNNCC 2 cut(s) 435, 1078
BspMAI CTGCAG 2 cut(s) 1153, 1168
BspQI GCTCTTC 1 cut(s) 563
BspT107I GGYRCC 1 cut(s) 1076
BspTI CTTAAG 1 cut(s) 1100
BsrDI GCAATG 1 cut(s) 45
BsrI ACTGG 1 cut(s) 599
BssECI CCNNGG 1 cut(s) 574
BssMI GATC 5 cut(s) 246, 667, 910, 960, 1068
Bst2UI CCWGG 2 cut(s) 431, 576
Bst4CI ACNGT 3 cut(s) 713, 774, 1035
Bst6I CTCTTC 3 cut(s) 317, 563, 819
BstAFI CTTAAG 1 cut(s) 1100
BstC8I GCNNGC 1 cut(s) 471
BstDEI CTNAG 6 cut(s) 99, 318, 395, 717, 1017, 1086
BstF5I GGATG 2 cut(s) 358, 939
BstFNI CGCG 2 cut(s) 192, 256
BstH2I RGCGCY 1 cut(s) 1176
BstHHI GCGC 1 cut(s) 1175
BstKTI GATC 5 cut(s) 249, 670, 913, 963, 1071
BstMBI GATC 5 cut(s) 246, 667, 910, 960, 1068
BstMWI GCNNNNNNNGC 5 cut(s) 1127, 1148, 1172, 1179, 1188
BstNI CCWGG 2 cut(s) 431, 576
BstPAI GACNNNNGTC 1 cut(s) 662
BstSCI CCNGG 2 cut(s) 429, 574
BstSFI CTRYAG 3 cut(s) 618, 1149, 1164
BstUI CGCG 2 cut(s) 192, 256
BstV1I GCAGC 6 cut(s) 1108, 1130, 1163, 1200, 1204, 1231
BstV2I GAAGAC 1 cut(s) 156
BstX2I RGATCY 1 cut(s) 910
BstXI CCANNNNNNTGG 1 cut(s) 594
BstYI RGATCY 1 cut(s) 910
BtrI CACGTC 1 cut(s) 924
BtsCI GGATG 2 cut(s) 358, 939
BtsIMutI CAGTG 1 cut(s) 1083
Cac8I GCNNGC 1 cut(s) 471
CaiI CAGNNNCTG 1 cut(s) 338
CfoI GCGC 1 cut(s) 1175
Cfr13I GGNCC 1 cut(s) 241
CseI GACGC 1 cut(s) 198
Csp6I GTAC 2 cut(s) 781, 1259
CspCI CAANNNNNGTGG 2 cut(s) 685, 720
CviAII CATG 2 cut(s) 388, 778
CviQI GTAC 2 cut(s) 781, 1259
DdeI CTNAG 6 cut(s) 99, 318, 395, 717, 1017, 1086
DpnI GATC 5 cut(s) 248, 669, 912, 962, 1070
DpnII GATC 5 cut(s) 246, 667, 910, 960, 1068
Eam1104I CTCTTC 3 cut(s) 317, 563, 819
EarI CTCTTC 3 cut(s) 317, 563, 819
Eco24I GRGCYC 1 cut(s) 575
Eco47I GGWCC 1 cut(s) 241
Eco57I CTGAAG 1 cut(s) 39
EcoRI GAATTC 1 cut(s) 895
EcoRII CCWGG 2 cut(s) 429, 574
EcoT38I GRGCYC 1 cut(s) 575
FaeI CATG 2 cut(s) 391, 781
FalI AAGNNNNNCTT 2 cut(s) 654, 686
FaqI GGGAC 1 cut(s) 1044
FatI CATG 2 cut(s) 387, 777
FbaI TGATCA 1 cut(s) 246
Fnu4HI GCNGC 9 cut(s) 193, 1119, 1122, 1152, 1183, 1186, 1189, 1218, 1245
FokI GGATG 2 cut(s) 365, 946
FriOI GRGCYC 1 cut(s) 575
Fsp4HI GCNGC 9 cut(s) 193, 1119, 1122, 1152, 1183, 1186, 1189, 1218, 1245
FspBI CTAG 4 cut(s) 203, 746, 794, 995
GlaI GCGC 1 cut(s) 1174
GluI GCNGC 9 cut(s) 193, 1119, 1122, 1152, 1183, 1186, 1189, 1218, 1245
HaeII RGCGCY 1 cut(s) 1176
HgaI GACGC 1 cut(s) 198
HhaI GCGC 1 cut(s) 1175
Hin1II CATG 2 cut(s) 391, 781
Hin6I GCGC 1 cut(s) 1173
HinP1I GCGC 1 cut(s) 1173
HindIII AAGCTT 1 cut(s) 101
HinfI GANTC 6 cut(s) 95, 329, 335, 372, 838, 1159
Hpy166II GTNNAC 3 cut(s) 241, 463, 976
Hpy188I TCNGA 6 cut(s) 169, 188, 334, 340, 630, 759
Hpy188III TCNNGA 6 cut(s) 158, 304, 380, 494, 560, 914
Hpy8I GTNNAC 3 cut(s) 241, 463, 976
Hpy99I CGWCG 3 cut(s) 192, 959, 1205
HpyAV CCTTC 2 cut(s) 229, 798
HpyCH4III ACNGT 3 cut(s) 713, 774, 1035
HpyCH4IV ACGT 3 cut(s) 287, 923, 1057
HpyCH4V TGCA 6 cut(s) 26, 686, 983, 1118, 1151, 1166
HpyF10VI GCNNNNNNNGC 5 cut(s) 1127, 1148, 1172, 1179, 1188
HpyF3I CTNAG 6 cut(s) 99, 318, 395, 717, 1017, 1086
HpySE526I ACGT 3 cut(s) 287, 923, 1057
Hsp92II CATG 2 cut(s) 391, 781
HspAI GCGC 1 cut(s) 1173
Ksp22I TGATCA 1 cut(s) 246
Kzo9I GATC 5 cut(s) 246, 667, 910, 960, 1068
LguI GCTCTTC 1 cut(s) 563
LmnI GCTCC 2 cut(s) 433, 1090
Lsp1109I GCAGC 6 cut(s) 1108, 1130, 1163, 1200, 1204, 1231
LweI GCATC 2 cut(s) 970, 1105
MaeI CTAG 4 cut(s) 203, 746, 794, 995
MaeII ACGT 3 cut(s) 287, 923, 1057
MaeIII GTNAC 2 cut(s) 707, 1012
MalI GATC 5 cut(s) 248, 669, 912, 962, 1070
MboI GATC 5 cut(s) 246, 667, 910, 960, 1068
MfeI CAATTG 1 cut(s) 648
MflI RGATCY 1 cut(s) 910
MhlI GDGCHC 1 cut(s) 575
MlyI GAGTC 2 cut(s) 104, 1153
MmeI TCCRAC 1 cut(s) 211
MnlI CCTC 7 cut(s) 318, 343, 478, 884, 1021, 1081, 1225
MroXI GAANNNNTTC 1 cut(s) 153
MseI TTAA 8 cut(s) 18, 410, 513, 581, 881, 951, 987, 1101
MslI CAYNNNNRTG 1 cut(s) 825
MspA1I CMGCKG 2 cut(s) 1121, 1182
MspCI CTTAAG 1 cut(s) 1100
MspR9I CCNGG 2 cut(s) 431, 576
MunI CAATTG 1 cut(s) 648
Mva1269I GAATGC 1 cut(s) 399
MvaI CCWGG 2 cut(s) 431, 576
MvnI CGCG 2 cut(s) 192, 256
MwoI GCNNNNNNNGC 5 cut(s) 1127, 1148, 1172, 1179, 1188
NdeII GATC 5 cut(s) 246, 667, 910, 960, 1068
NlaIII CATG 2 cut(s) 391, 781
NlaIV GGNNCC 2 cut(s) 435, 1078
OliI CACNNNNGTG 1 cut(s) 825
PciSI GCTCTTC 1 cut(s) 563
PctI GAATGC 1 cut(s) 399
PdmI GAANNNNTTC 1 cut(s) 153
PfeI GAWTC 4 cut(s) 329, 335, 372, 838
PfoI TCCNGGA 1 cut(s) 429
PkrI GCNGC 9 cut(s) 194, 1120, 1123, 1153, 1184, 1187, 1190, 1219, 1246
PleI GAGTC 2 cut(s) 103, 1153
PpsI GAGTC 2 cut(s) 103, 1153
PshAI GACNNNNGTC 1 cut(s) 662
Psp6I CCWGG 2 cut(s) 429, 574
PspGI CCWGG 2 cut(s) 429, 574
PspN4I GGNNCC 2 cut(s) 435, 1078
PspPI GGNCC 1 cut(s) 241
PsrI GAACNNNNNNTAC 2 cut(s) 489, 521
PstI CTGCAG 2 cut(s) 1153, 1168
PstNI CAGNNNCTG 1 cut(s) 338
PsuI RGATCY 1 cut(s) 910
PvuII CAGCTG 1 cut(s) 1121
RsaI GTAC 2 cut(s) 782, 1260
RsaNI GTAC 2 cut(s) 781, 1259
RseI CAYNNNNRTG 1 cut(s) 825
SapI GCTCTTC 1 cut(s) 563
SaqAI TTAA 8 cut(s) 18, 410, 513, 581, 881, 951, 987, 1101
SatI GCNGC 9 cut(s) 193, 1119, 1122, 1152, 1183, 1186, 1189, 1218, 1245
Sau3AI GATC 5 cut(s) 246, 667, 910, 960, 1068
Sau96I GGNCC 1 cut(s) 241
SchI GAGTC 2 cut(s) 104, 1153
ScrFI CCNGG 2 cut(s) 431, 576
SduI GDGCHC 1 cut(s) 575
SfaNI GCATC 2 cut(s) 970, 1105
SfcI CTRYAG 3 cut(s) 618, 1149, 1164
SinI GGWCC 1 cut(s) 241
SmiMI CAYNNNNRTG 1 cut(s) 825
SmlI CTYRAG 1 cut(s) 1100
SmoI CTYRAG 1 cut(s) 1100
SsiI CCGC 4 cut(s) 192, 801, 1182, 1185
SspI AATATT 2 cut(s) 128, 529
SspMI CTAG 4 cut(s) 203, 746, 794, 995
StyD4I CCNGG 2 cut(s) 429, 574
TaaI ACNGT 3 cut(s) 713, 774, 1035
TaiI ACGT 3 cut(s) 290, 926, 1060
TaqI TCGA 1 cut(s) 666
TatI WGTACW 1 cut(s) 1258
TauI GCSGC 3 cut(s) 195, 1185, 1188
TfiI GAWTC 4 cut(s) 329, 335, 372, 838
Tru1I TTAA 8 cut(s) 18, 410, 513, 581, 881, 951, 987, 1101
Tru9I TTAA 8 cut(s) 18, 410, 513, 581, 881, 951, 987, 1101
TscAI CASTG 1 cut(s) 1090
TseI GCWGC 6 cut(s) 1118, 1121, 1151, 1188, 1217, 1244
TspDTI ATGAA 4 cut(s) 48, 595, 900, 953
TspGWI ACGGA 1 cut(s) 365
TspRI CASTG 1 cut(s) 1090
Vha464I CTTAAG 1 cut(s) 1100
VpaK11BI GGWCC 1 cut(s) 241
XapI RAATTY 3 cut(s) 296, 406, 895
XmnI GAANNNNTTC 1 cut(s) 153
XspI CTAG 4 cut(s) 203, 746, 794, 995
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.