Rroxscaffold_2G00076280

Protein of unknown function (DUF3754)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000718
Physical Location & Seq
Reverse (-)
184587 .. 189803
5217 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00076280.1

Sequence Viewer

Length: 1482 bp
ATGGGCAAGGGCAAGAAAGATATTATTCGATTAGAGCGTGAATCCGTCATTGCAGTTATTAAGCCCAGACTCATCATGACCTTGACCAATCTCATCGAGCAAAGTTCTGATCGGACTGAGTTTTTGAAGCTATGCAAGAGGGTTGAGTACACAATTCGGGCTTGGTATCTTCTACAATTTGAGGATTTGATGCAACTGTACTCCCTCTTTGATCCTGTACATGGAGCCCAGAGGTTGGAGCAACAGAAACTTGACGATGAAGAAATTGATGTGCTTGAACAAAATTTCCTCATGTACTTATTTCAGGTGATGGAAAAAAGTAATTTTAAGATTGCAAATAATGAAGAAATTGAGGTTGCGGCTTCAGGGCAGTATCTTCTACGTCTTCCCATTGTGGTTGATGAGTCTAAGCTTGACAACAGACTTTTGAAGAAATATTTCGAAGGTCATCCTACCGAAGACATTCCAGATTTTGCTGATAAGTATGTCATCTTCCGACGCGGCATTGGACTTGATAAGACAACCGGTTGGTTTTTCATGGAGAAGGTGGACATTATCATTGCACGTTTTTGGAAATATATTTTACAAATAACAAAGTTAGACAAGTTTTTCAAGAAACCAACCCCTAAGAAGAGGGATTCAGAATCTGATACAGATGAGGACGACTTCTTTGTTGAACGAATCCGTCTTGAAAACATGGAACTAAGCGTTCAAACTTTGCTTCTTAAGAACACAATCCAGGAGCCAACTTTTGATAGGATTATCGTTGTTTACAGACAAGCAACTACCGACGAGGATGGTTCAGAACGGGGAATACACATTAAACATTTCAAAAATATTCCAATGGCTGATCTGGAGATAGTTCTTCCTGAAAAGAAGAACCCAGGTTTAACACCAATGGACTGGCTCTCGTTCATAGGCTCTGCTGTAGTTGGTCTGGTTGCTGTTATTACTTCGATTGAAACGACTAAAGTCGATCTTTGGGTTATGTTTGCAATTCTTTCCACTGTGATTGGTTACTGCGCTAAGACATATTTCAGTTTTCAGCAGAACTTAGCTCAATATCAGAACTTGATAACACGATCCATGTACGAAAAACAACTAGATAGTGGAAGGGGAACTCTTCTTCACTTGTGTGATGATGTGATTCAACAGGAAGTCAAGGAAGTAATAGTTTCTTTCTATATGTTAATGGAACAAGGGAAAGCTACTTTACCTGATCTGGACAAGTGGTGTGAGGACCTAATCAAAGAACAGTTTAACGACGATATCAATTTTGATGTGGACGATGCAGTTAAGAAGTTAGAGAAATTGGGAATTGTTACCCGGGATACCCTTGGACGGTATAGCGCTGTTGGGCTGAAACGTGCTAACGAGATCATTGGCACCACCACTGAGGAGGTTGTCCTCAAGGCAAAACAAGATGCTGCTAATGCTCCTGCTTCTTCTGCAGCTGCTGCGGTCGCTGCCGCTGGTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3754

Protein Analysis

493

Amino Acids

56.69

Weight (kDa)

5.09

Isoelectric Point (pI)

29.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3754 PF12576 251 - 373 4.2e-24 Protein of unknown function (DUF3754)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1385
AccB7I CCANNNNNTGG 1 cut(s) 235
AccII CGCG 1 cut(s) 501
AciI CCGC 4 cut(s) 359, 501, 1460, 1470
AclWI GGATC 2 cut(s) 206, 1077
AcsI RAATTY 1 cut(s) 283
AcuI CTGAAG 1 cut(s) 348
AfaI GTAC 5 cut(s) 149, 200, 219, 296, 1091
AfeI AGCGCT 1 cut(s) 1351
AfiI CCNNNNNNNGG 3 cut(s) 221, 235, 1341
AflII CTTAAG 1 cut(s) 725
AgeI ACCGGT 1 cut(s) 524
AjnI CCWGG 2 cut(s) 738, 883
AleI CACNNNNGTG 1 cut(s) 1134
AloI GAACNNNNNNTCC 2 cut(s) 270, 302
AluBI AGCT 5 cut(s) 130, 412, 1058, 1208, 1454
AluI AGCT 5 cut(s) 130, 412, 1058, 1208, 1454
AlwI GGATC 2 cut(s) 206, 1077
AlwNI CAGNNNCTG 2 cut(s) 647, 1457
Ama87I CYCGRG 1 cut(s) 1326
Aor51HI AGCGCT 1 cut(s) 1351
ApeKI GCWGC 5 cut(s) 1427, 1451, 1454, 1457, 1466
ApoI RAATTY 1 cut(s) 283
ArsI GACNNNNNNTTYG 4 cut(s) 106, 138, 653, 685
AsiGI ACCGGT 1 cut(s) 524
Asp700I GAANNNNTTC 2 cut(s) 437, 462
AspLEI GCGC 2 cut(s) 1025, 1352
AspS9I GGNCC 1 cut(s) 1240
AsuC2I CCSGG 2 cut(s) 1327, 1328
AsuHPI GGTGA 1 cut(s) 319
AsuII TTCGAA 1 cut(s) 441
AvaI CYCGRG 1 cut(s) 1326
AvaII GGWCC 1 cut(s) 1240
BanI GGYRCC 1 cut(s) 1385
BanII GRGCYC 1 cut(s) 229
BarI GAAGNNNNNNTAC 2 cut(s) 476, 508
BbsI GAAGAC 2 cut(s) 377, 465
BbvI GCAGC 5 cut(s) 1414, 1441, 1444, 1453, 1463
BccI CCATC 2 cut(s) 304, 791
BciT130I CCWGG 2 cut(s) 740, 885
BciVI GTATCC 1 cut(s) 1324
BcnI CCSGG 2 cut(s) 1327, 1328
BfaI CTAG 1 cut(s) 1103
BfmI CTRYAG 2 cut(s) 927, 1449
BfoI RGCGCY 1 cut(s) 1353
BfrI CTTAAG 1 cut(s) 725
BfuI GTATCC 1 cut(s) 1324
BisI GCNGC 8 cut(s) 360, 502, 1428, 1452, 1455, 1458, 1467, 1470
BlsI GCNGC 8 cut(s) 361, 503, 1429, 1453, 1456, 1459, 1468, 1471
Bme1390I CCNGG 4 cut(s) 740, 885, 1327, 1328
Bme18I GGWCC 1 cut(s) 1240
BmeT110I CYCGRG 1 cut(s) 1326
BmgT120I GGNCC 1 cut(s) 1240
BmiI GGNNCC 3 cut(s) 226, 744, 1387
BmrFI CCNGG 4 cut(s) 740, 885, 1327, 1328
BmsI GCATC 3 cut(s) 180, 1279, 1414
BoxI GACNNNNGTC 1 cut(s) 971
BpiI GAAGAC 2 cut(s) 377, 465
BpmI CTGGAG 1 cut(s) 875
Bpu14I TTCGAA 1 cut(s) 441
BpuEI CTTGAG 1 cut(s) 1394
BpuMI CCSGG 2 cut(s) 1327, 1328
BsaJI CCNNGG 3 cut(s) 883, 1326, 1336
BsaWI WCCGGW 1 cut(s) 524
Bsc4I CCNNNNNNNGG 3 cut(s) 221, 235, 1341
Bse118I RCCGGY 1 cut(s) 524
Bse1I ACTGG 1 cut(s) 908
Bse3DI GCAATG 2 cut(s) 48, 558
BseBI CCWGG 2 cut(s) 740, 885
BseDI CCNNGG 3 cut(s) 883, 1326, 1336
BseGI GGATG 2 cut(s) 448, 802
BseLI CCNNNNNNNGG 3 cut(s) 221, 235, 1341
BseMI GCAATG 2 cut(s) 48, 558
BseMII CTCAG 2 cut(s) 108, 1386
BseNI ACTGG 1 cut(s) 908
BseRI GAGGAG 1 cut(s) 1412
BseXI GCAGC 5 cut(s) 1414, 1441, 1444, 1453, 1463
Bsh1236I CGCG 1 cut(s) 501
Bsh1285I CGRYCG 1 cut(s) 1464
BshNI GGYRCC 1 cut(s) 1385
BshTI ACCGGT 1 cut(s) 524
BsiEI CGRYCG 1 cut(s) 1464
BsiHKCI CYCGRG 1 cut(s) 1326
BsiSI CCGG 2 cut(s) 525, 1327
BslI CCNNNNNNNGG 3 cut(s) 221, 235, 1341
BsoBI CYCGRG 1 cut(s) 1326
Bsp119I TTCGAA 1 cut(s) 441
Bsp1286I GDGCHC 1 cut(s) 229
Bsp1407I TGTACA 1 cut(s) 217
Bsp143I GATC 7 cut(s) 109, 211, 850, 976, 1082, 1219, 1377
BspACI CCGC 4 cut(s) 359, 501, 1460, 1470
BspCNI CTCAG 2 cut(s) 109, 1387
BspFNI CGCG 1 cut(s) 501
BspHI TCATGA 1 cut(s) 75
BspLI GGNNCC 3 cut(s) 226, 744, 1387
BspMAI CTGCAG 1 cut(s) 1453
BspPI GGATC 2 cut(s) 206, 1077
BspT104I TTCGAA 1 cut(s) 441
BspT107I GGYRCC 1 cut(s) 1385
BspTI CTTAAG 1 cut(s) 725
BsrDI GCAATG 2 cut(s) 48, 558
BsrFI RCCGGY 1 cut(s) 524
BsrGI TGTACA 1 cut(s) 217
BsrI ACTGG 1 cut(s) 908
BssAI RCCGGY 1 cut(s) 524
BssECI CCNNGG 3 cut(s) 883, 1326, 1336
BssMI GATC 7 cut(s) 109, 211, 850, 976, 1082, 1219, 1377
BssT1I CCWWGG 1 cut(s) 1336
Bst2UI CCWGG 2 cut(s) 740, 885
Bst4CI ACNGT 4 cut(s) 198, 1009, 1257, 1344
Bst6I CTCTTC 2 cut(s) 626, 1128
BstAFI CTTAAG 1 cut(s) 725
BstAPI GCANNNNNTGC 1 cut(s) 1457
BstAUI TGTACA 1 cut(s) 217
BstBI TTCGAA 1 cut(s) 441
BstDEI CTNAG 7 cut(s) 117, 408, 627, 704, 1026, 1054, 1395
BstF5I GGATG 2 cut(s) 448, 802
BstFNI CGCG 1 cut(s) 501
BstH2I RGCGCY 1 cut(s) 1353
BstHHI GCGC 2 cut(s) 1025, 1352
BstKTI GATC 7 cut(s) 112, 214, 853, 979, 1085, 1222, 1380
BstMBI GATC 7 cut(s) 109, 211, 850, 976, 1082, 1219, 1377
BstMCI CGRYCG 1 cut(s) 1464
BstMWI GCNNNNNNNGC 5 cut(s) 1433, 1448, 1457, 1463, 1466
BstNI CCWGG 2 cut(s) 740, 885
BstPAI GACNNNNGTC 1 cut(s) 971
BstSCI CCNGG 4 cut(s) 738, 883, 1325, 1326
BstSFI CTRYAG 2 cut(s) 927, 1449
BstUI CGCG 1 cut(s) 501
BstV1I GCAGC 5 cut(s) 1414, 1441, 1444, 1453, 1463
BstV2I GAAGAC 2 cut(s) 377, 465
BstXI CCANNNNNNTGG 1 cut(s) 903
BsuI GTATCC 1 cut(s) 1324
BtsCI GGATG 2 cut(s) 448, 802
BtsIMutI CAGTG 2 cut(s) 1005, 1392
CaiI CAGNNNCTG 2 cut(s) 647, 1457
CciI TCATGA 1 cut(s) 75
CfoI GCGC 2 cut(s) 1025, 1352
Cfr10I RCCGGY 1 cut(s) 524
Cfr13I GGNCC 1 cut(s) 1240
Cfr9I CCCGGG 1 cut(s) 1326
CseI GACGC 1 cut(s) 507
Csp6I GTAC 5 cut(s) 148, 199, 218, 295, 1090
CspAI ACCGGT 1 cut(s) 524
CspCI CAANNNNNGTGG 2 cut(s) 994, 1029
CviAII CATG 6 cut(s) 76, 221, 292, 538, 697, 1087
CviQI GTAC 5 cut(s) 148, 199, 218, 295, 1090
DdeI CTNAG 7 cut(s) 117, 408, 627, 704, 1026, 1054, 1395
DpnI GATC 7 cut(s) 111, 213, 852, 978, 1084, 1221, 1379
DpnII GATC 7 cut(s) 109, 211, 850, 976, 1082, 1219, 1377
Eam1104I CTCTTC 2 cut(s) 626, 1128
EarI CTCTTC 2 cut(s) 626, 1128
Eco130I CCWWGG 1 cut(s) 1336
Eco24I GRGCYC 1 cut(s) 229
Eco32I GATATC 1 cut(s) 1270
Eco47I GGWCC 1 cut(s) 1240
Eco47III AGCGCT 1 cut(s) 1351
Eco57I CTGAAG 1 cut(s) 348
Eco88I CYCGRG 1 cut(s) 1326
EcoO109I RGGNCCY 1 cut(s) 1240
EcoRII CCWGG 2 cut(s) 738, 883
EcoRV GATATC 1 cut(s) 1270
EcoT14I CCWWGG 1 cut(s) 1336
EcoT38I GRGCYC 1 cut(s) 229
ErhI CCWWGG 1 cut(s) 1336
FaeI CATG 6 cut(s) 79, 224, 295, 541, 700, 1090
FalI AAGNNNNNCTT 2 cut(s) 963, 995
FatI CATG 6 cut(s) 75, 220, 291, 537, 696, 1086
Fnu4HI GCNGC 8 cut(s) 360, 502, 1428, 1452, 1455, 1458, 1467, 1470
FokI GGATG 2 cut(s) 435, 809
FriOI GRGCYC 1 cut(s) 229
Fsp4HI GCNGC 8 cut(s) 360, 502, 1428, 1452, 1455, 1458, 1467, 1470
FspBI CTAG 1 cut(s) 1103
GlaI GCGC 2 cut(s) 1024, 1351
GluI GCNGC 8 cut(s) 360, 502, 1428, 1452, 1455, 1458, 1467, 1470
GsuI CTGGAG 1 cut(s) 875
HaeII RGCGCY 1 cut(s) 1353
HapII CCGG 2 cut(s) 525, 1327
HgaI GACGC 1 cut(s) 507
HhaI GCGC 2 cut(s) 1025, 1352
Hin1II CATG 6 cut(s) 79, 224, 295, 541, 700, 1090
Hin6I GCGC 2 cut(s) 1023, 1350
HinP1I GCGC 2 cut(s) 1023, 1350
HindIII AAGCTT 1 cut(s) 410
HinfI GANTC 7 cut(s) 41, 69, 404, 638, 644, 681, 1147
HpaII CCGG 2 cut(s) 525, 1327
HphI GGTGA 1 cut(s) 319
Hpy166II GTNNAC 4 cut(s) 150, 550, 772, 1285
Hpy188I TCNGA 7 cut(s) 109, 114, 497, 643, 649, 805, 1068
Hpy188III TCNNGA 7 cut(s) 76, 467, 613, 689, 854, 869, 1223
Hpy8I GTNNAC 4 cut(s) 150, 550, 772, 1285
Hpy99I CGWCG 3 cut(s) 501, 794, 1268
HpyAV CCTTC 3 cut(s) 437, 538, 1107
HpyCH4III ACNGT 4 cut(s) 198, 1009, 1257, 1344
HpyCH4IV ACGT 3 cut(s) 382, 565, 1366
HpyCH4V TGCA 8 cut(s) 53, 135, 193, 335, 563, 995, 1292, 1451
HpyF10VI GCNNNNNNNGC 5 cut(s) 1433, 1448, 1457, 1463, 1466
HpyF3I CTNAG 7 cut(s) 117, 408, 627, 704, 1026, 1054, 1395
HpySE526I ACGT 3 cut(s) 382, 565, 1366
Hsp92II CATG 6 cut(s) 79, 224, 295, 541, 700, 1090
HspAI GCGC 2 cut(s) 1023, 1350
Kzo9I GATC 7 cut(s) 109, 211, 850, 976, 1082, 1219, 1377
LmnI GCTCC 4 cut(s) 224, 238, 742, 1441
Lsp1109I GCAGC 5 cut(s) 1414, 1441, 1444, 1453, 1463
LweI GCATC 3 cut(s) 180, 1279, 1414
MaeI CTAG 1 cut(s) 1103
MaeII ACGT 3 cut(s) 382, 565, 1366
MaeIII GTNAC 2 cut(s) 1016, 1321
MalI GATC 7 cut(s) 111, 213, 852, 978, 1084, 1221, 1379
MboI GATC 7 cut(s) 109, 211, 850, 976, 1082, 1219, 1377
MhlI GDGCHC 1 cut(s) 229
MlyI GAGTC 2 cut(s) 63, 413
MmeI TCCRAC 2 cut(s) 216, 520
MroXI GAANNNNTTC 2 cut(s) 437, 462
MseI TTAA 9 cut(s) 60, 327, 726, 822, 890, 1190, 1260, 1296, 1480
MslI CAYNNNNRTG 1 cut(s) 1134
MspA1I CMGCKG 2 cut(s) 1454, 1472
MspCI CTTAAG 1 cut(s) 725
MspI CCGG 2 cut(s) 525, 1327
MspR9I CCNGG 4 cut(s) 740, 885, 1327, 1328
MvaI CCWGG 2 cut(s) 740, 885
MvnI CGCG 1 cut(s) 501
MwoI GCNNNNNNNGC 5 cut(s) 1433, 1448, 1457, 1463, 1466
NciI CCSGG 2 cut(s) 1327, 1328
NdeII GATC 7 cut(s) 109, 211, 850, 976, 1082, 1219, 1377
NlaIII CATG 6 cut(s) 79, 224, 295, 541, 700, 1090
NlaIV GGNNCC 3 cut(s) 226, 744, 1387
NspV TTCGAA 1 cut(s) 441
OliI CACNNNNGTG 1 cut(s) 1134
PagI TCATGA 1 cut(s) 75
PcsI WCGNNNNNNNCGW 2 cut(s) 34, 962
PdmI GAANNNNTTC 2 cut(s) 437, 462
PfeI GAWTC 5 cut(s) 41, 638, 644, 681, 1147
PflMI CCANNNNNTGG 1 cut(s) 235
PfoI TCCNGGA 1 cut(s) 738
PinAI ACCGGT 1 cut(s) 524
PkrI GCNGC 8 cut(s) 361, 503, 1429, 1453, 1456, 1459, 1468, 1471
PleI GAGTC 2 cut(s) 63, 412
PpsI GAGTC 2 cut(s) 63, 412
PpuMI RGGWCCY 1 cut(s) 1240
PshAI GACNNNNGTC 1 cut(s) 971
Psp5II RGGWCCY 1 cut(s) 1240
Psp6I CCWGG 2 cut(s) 738, 883
PspGI CCWGG 2 cut(s) 738, 883
PspN4I GGNNCC 3 cut(s) 226, 744, 1387
PspPI GGNCC 1 cut(s) 1240
PspPPI RGGWCCY 1 cut(s) 1240
PsrI GAACNNNNNNTAC 2 cut(s) 798, 830
PstI CTGCAG 1 cut(s) 1453
PstNI CAGNNNCTG 2 cut(s) 647, 1457
PvuII CAGCTG 1 cut(s) 1454
RsaI GTAC 5 cut(s) 149, 200, 219, 296, 1091
RsaNI GTAC 5 cut(s) 148, 199, 218, 295, 1090
RseI CAYNNNNRTG 1 cut(s) 1134
SaqAI TTAA 9 cut(s) 60, 327, 726, 822, 890, 1190, 1260, 1296, 1480
SatI GCNGC 8 cut(s) 360, 502, 1428, 1452, 1455, 1458, 1467, 1470
Sau3AI GATC 7 cut(s) 109, 211, 850, 976, 1082, 1219, 1377
Sau96I GGNCC 1 cut(s) 1240
SchI GAGTC 2 cut(s) 63, 413
ScrFI CCNGG 4 cut(s) 740, 885, 1327, 1328
SduI GDGCHC 1 cut(s) 229
SfaNI GCATC 3 cut(s) 180, 1279, 1414
SfcI CTRYAG 2 cut(s) 927, 1449
SfuI TTCGAA 1 cut(s) 441
SinI GGWCC 1 cut(s) 1240
SmaI CCCGGG 1 cut(s) 1328
SmiMI CAYNNNNRTG 1 cut(s) 1134
SmlI CTYRAG 2 cut(s) 725, 1409
SmoI CTYRAG 2 cut(s) 725, 1409
SsiI CCGC 4 cut(s) 359, 501, 1460, 1470
SspI AATATT 2 cut(s) 437, 838
SspMI CTAG 1 cut(s) 1103
StyD4I CCNGG 4 cut(s) 738, 883, 1325, 1326
StyI CCWWGG 1 cut(s) 1336
TaaI ACNGT 4 cut(s) 198, 1009, 1257, 1344
TaiI ACGT 3 cut(s) 385, 568, 1369
TaqI TCGA 5 cut(s) 28, 96, 441, 956, 975
TatI WGTACW 4 cut(s) 147, 198, 217, 294
TauI GCSGC 3 cut(s) 362, 504, 1472
TfiI GAWTC 5 cut(s) 41, 638, 644, 681, 1147
Tru1I TTAA 9 cut(s) 60, 327, 726, 822, 890, 1190, 1260, 1296, 1480
Tru9I TTAA 9 cut(s) 60, 327, 726, 822, 890, 1190, 1260, 1296, 1480
TscAI CASTG 2 cut(s) 1012, 1399
TseI GCWGC 5 cut(s) 1427, 1451, 1454, 1457, 1466
TspDTI ATGAA 4 cut(s) 273, 357, 526, 904
TspGWI ACGGA 2 cut(s) 34, 674
TspMI CCCGGG 1 cut(s) 1326
TspRI CASTG 2 cut(s) 1012, 1399
Van91I CCANNNNNTGG 1 cut(s) 235
Vha464I CTTAAG 1 cut(s) 725
VpaK11BI GGWCC 1 cut(s) 1240
XapI RAATTY 1 cut(s) 283
XmaI CCCGGG 1 cut(s) 1326
XmnI GAANNNNTTC 2 cut(s) 437, 462
XspI CTAG 1 cut(s) 1103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.