Rh2DG648400

Protein of unknown function (DUF3754)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000718
Physical Location & Seq
Reverse (-)
87479409 .. 87485167
5759 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG648400.1

Sequence Viewer

Length: 1527 bp
ATGTGTGGTCTTGCTTGGGTCTCACCTCTTCTCCCCCCGTTTTGCAAGAAACTGACACACCAAAACAGTAAAAGATACCAAACCCTCTCCCTCCCTTTACTGTCAAGAATAAACCGTTCCTCTCCTCTCTCTCTCACATTTAAACACCCTCAATCCGTTTCCATTGGCGTTCAAGAACCCGGAAATTCGTTTCTGCAGAGACCCAGAATGGAGAAAACCGCGTCCCAGAAGAAGAAAGACATGATTCGAATAGAGCGGGAGTCAGTGATTCCGGTTCTCAAGCCCAAGCTCATCATGTCCTTGGCCAACCTCATTGAACATAGTTCCGACCGGGCGGAGTTTTTAAAGCTCAGCAAGAGAATTGAGTACACGATTCGGGCTTGGTATCTTCTACAATTCGAGGATTTGATGCAACTGTATTCGCTCTTTGACCCCGTACATGGGAATCAAAAATTGGAGCAACAGAATCTTTCTCCTAAAGAGATTGATGTACTGGAACAGAATTTCCTCACGTACTTACTTCAGGTGATGGAAAAGAGTAATTTCAAGATTGCAACTGATGAAGAAATTGAGATTGCACATTCAGGGCAGTATCTTCTAAATCTTCCCATCGTGGTTGATGAATCTAAGATTGACAAGAAGCTTCTGAAGAACTTCTTTGCAGAGAATCCTCAGCCAAACCTTCCAGATTTTGCTGATAAGTACATTATCTTCCGGCGTGGTGTTGGACTTGATCAGACAACTGATTACTTTTTCATGGAGAAAGTGGACATGATCATTGCACGTTTTTGGGCATATCTTTTACGAATAACTAGGGCTGAAAAACTTTTATCCAGAAGGTCAAGTAGTCGGCACACAAAAGATCCAAAGAAGGATGATGAAATTACCTCAGAAGCAGATGGGGAGGAGGCGGAGGACTTATACGTTGAACGAATTCGTCTTGAAAATATGGAACTAAGCGTTAAAAGCTTGCTGAGCAAGACCACAATCAAAGAGCCAACCTTTGATAGGATAATTGTTGTTTACAGGCGAGCAAGTACCAAAGCAAAAGAAGAACGAGGAATATTTGTGAAACATTTCAAAAATATTCCCATGGCTGATATGGAGATAGTTCTTCCCGAGAAGAAGAACCCAGGATTAACTCCAATGGACTGGGTCAAGTTCCTTGGCTCTGCTGTAGTTGGGCTGGTTGCTGTTATTAGTTCGCTTGAAATGCCTAATGCTGACTTTTTGGTCATCATTGCGGTTCTTTCCACTGTCATTGGTTACTGTGCTAAGATATACTTCAGTTTTCAGCAGAACTTGGCTGCATATCAGAACCTAATAACACAGTCCATGTATGACAAGCAACTGGATAGTGGAAGGGGTACTCTTCTTCACTTGTGTGATGATGTGATTCAACAGGAGGTCAAGGAGGTGATACTTTCTTTCTATATATTAATGGAACAGGGAAAAGCTACAAGAGAAGTACGCCATCTTTCTTCTTTGGTTCTGTTTATAAATTCCCTAATTATTGCAAGAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3754

Protein Analysis

508

Amino Acids

58.56

Weight (kDa)

8.7

Isoelectric Point (pI)

40.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3754 PF12576 335 - 456 4.4e-23 Protein of unknown function (DUF3754)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1499
AasI GACNNNNNNGTC 1 cut(s) 1232
AccBSI CCGCTC 1 cut(s) 256
AccII CGCG 1 cut(s) 221
AciI CCGC 5 cut(s) 219, 256, 335, 911, 1244
AclWI GGATC 1 cut(s) 857
AcoI YGGCCR 1 cut(s) 303
AcsI RAATTY 4 cut(s) 184, 502, 933, 1501
AcuI CTGAAG 3 cut(s) 506, 668, 1270
AfaI GTAC 8 cut(s) 368, 438, 492, 515, 704, 1039, 1369, 1470
AfiI CCNNNNNNNGG 3 cut(s) 440, 441, 1008
AgsI TTSAA 8 cut(s) 173, 317, 547, 929, 944, 1081, 1211, 1400
AjnI CCWGG 1 cut(s) 1132
AjuI GAANNNNNNNTTGG 2 cut(s) 437, 469
AleI CACNNNNGTG 1 cut(s) 1383
AloI GAACNNNNNNTCC 2 cut(s) 489, 521
AluBI AGCT 5 cut(s) 289, 349, 643, 969, 1457
AluI AGCT 5 cut(s) 289, 349, 643, 969, 1457
Alw26I GTCTC 2 cut(s) 25, 193
AlwI GGATC 1 cut(s) 857
Ama87I CYCGRG 1 cut(s) 1118
AoxI GGCC 1 cut(s) 303
ApeKI GCWGC 1 cut(s) 1307
ApoI RAATTY 4 cut(s) 184, 502, 933, 1501
AseI ATTAAT 1 cut(s) 1439
Asp700I GAANNNNTTC 3 cut(s) 653, 933, 1076
AsuC2I CCSGG 2 cut(s) 180, 332
AsuHPI GGTGA 3 cut(s) 15, 538, 1429
AsuII TTCGAA 1 cut(s) 247
AvaI CYCGRG 1 cut(s) 1118
BalI TGGCCA 1 cut(s) 305
BarI GAAGNNNNNNTAC 2 cut(s) 695, 727
BbvCI CCTCAGC 1 cut(s) 672
BbvI GCAGC 1 cut(s) 1294
BccI CCATC 4 cut(s) 523, 617, 893, 1482
BciT130I CCWGG 1 cut(s) 1134
BclI TGATCA 2 cut(s) 733, 774
BcnI CCSGG 2 cut(s) 180, 332
BcoDI GTCTC 2 cut(s) 25, 193
BfaI CTAG 1 cut(s) 813
BfmI CTRYAG 2 cut(s) 194, 1176
BisI GCNGC 1 cut(s) 1308
BlpI GCTNAGC 2 cut(s) 350, 974
BlsI GCNGC 1 cut(s) 1309
Bme1390I CCNGG 3 cut(s) 180, 332, 1134
BmeT110I CYCGRG 1 cut(s) 1118
BmrFI CCNGG 3 cut(s) 180, 332, 1134
BmrI ACTGGG 1 cut(s) 1162
BmsI GCATC 1 cut(s) 399
BmuI ACTGGG 1 cut(s) 1162
Bpu10I CCTNAGC 1 cut(s) 672
Bpu1102I GCTNAGC 2 cut(s) 350, 974
Bpu14I TTCGAA 1 cut(s) 247
BpuEI CTTGAG 1 cut(s) 263
BpuMI CCSGG 2 cut(s) 180, 332
BsaAI YACGTR 1 cut(s) 513
BsaI GGTCTC 2 cut(s) 25, 193
BsaJI CCNNGG 4 cut(s) 300, 1092, 1132, 1165
BsaWI WCCGGW 1 cut(s) 271
BsaXI ACNNNNNCTCC 2 cut(s) 15, 45
Bsc4I CCNNNNNNNGG 3 cut(s) 440, 441, 1008
Bse1I ACTGG 3 cut(s) 498, 1157, 1356
Bse3DI GCAATG 2 cut(s) 777, 1239
BseBI CCWGG 1 cut(s) 1134
BseDI CCNNGG 4 cut(s) 300, 1092, 1132, 1165
BseGI GGATG 1 cut(s) 880
BseLI CCNNNNNNNGG 3 cut(s) 440, 441, 1008
BseMI GCAATG 2 cut(s) 777, 1239
BseMII CTCAG 4 cut(s) 364, 686, 903, 965
BseNI ACTGG 3 cut(s) 498, 1157, 1356
BseRI GAGGAG 2 cut(s) 114, 920
BseXI GCAGC 1 cut(s) 1294
Bsh1236I CGCG 1 cut(s) 221
Bsh1285I CGRYCG 1 cut(s) 331
BshFI GGCC 1 cut(s) 305
BsiEI CGRYCG 1 cut(s) 331
BsiHKCI CYCGRG 1 cut(s) 1118
BsiSI CCGG 4 cut(s) 180, 272, 331, 715
BslFI GGGAC 1 cut(s) 208
BslI CCNNNNNNNGG 3 cut(s) 440, 441, 1008
BsmAI GTCTC 2 cut(s) 25, 193
BsmFI GGGAC 1 cut(s) 208
BsnI GGCC 1 cut(s) 305
Bso31I GGTCTC 2 cut(s) 25, 193
BsoBI CYCGRG 1 cut(s) 1118
Bsp119I TTCGAA 1 cut(s) 247
Bsp143I GATC 3 cut(s) 733, 774, 862
Bsp1720I GCTNAGC 2 cut(s) 350, 974
Bsp19I CCATGG 1 cut(s) 1092
BspACI CCGC 5 cut(s) 219, 256, 335, 911, 1244
BspANI GGCC 1 cut(s) 305
BspCNI CTCAG 4 cut(s) 363, 685, 902, 966
BspFNI CGCG 1 cut(s) 221
BspMAI CTGCAG 1 cut(s) 198
BspPI GGATC 1 cut(s) 857
BspT104I TTCGAA 1 cut(s) 247
BspTNI GGTCTC 2 cut(s) 25, 193
BsrBI CCGCTC 1 cut(s) 256
BsrDI GCAATG 2 cut(s) 777, 1239
BsrI ACTGG 3 cut(s) 498, 1157, 1356
BssECI CCNNGG 4 cut(s) 300, 1092, 1132, 1165
BssMI GATC 3 cut(s) 733, 774, 862
BssT1I CCWWGG 3 cut(s) 300, 1092, 1165
Bst2UI CCWGG 1 cut(s) 1134
Bst4CI ACNGT 7 cut(s) 68, 102, 116, 417, 1258, 1271, 1332
Bst6I CTCTTC 2 cut(s) 33, 1377
BstBAI YACGTR 1 cut(s) 513
BstBI TTCGAA 1 cut(s) 247
BstC8I GCNNGC 2 cut(s) 971, 1032
BstDEI CTNAG 7 cut(s) 350, 627, 672, 889, 956, 974, 1275
BstDSI CCRYGG 1 cut(s) 1092
BstENI CCTNNNNNAGG 1 cut(s) 1006
BstF5I GGATG 1 cut(s) 880
BstFNI CGCG 1 cut(s) 221
BstKTI GATC 3 cut(s) 736, 777, 865
BstMAI GTCTC 2 cut(s) 25, 193
BstMBI GATC 3 cut(s) 733, 774, 862
BstMCI CGRYCG 1 cut(s) 331
BstMWI GCNNNNNNNGC 3 cut(s) 966, 975, 1213
BstNI CCWGG 1 cut(s) 1134
BstSCI CCNGG 3 cut(s) 178, 330, 1132
BstSFI CTRYAG 2 cut(s) 194, 1176
BstUI CGCG 1 cut(s) 221
BstV1I GCAGC 1 cut(s) 1294
BstX2I RGATCY 1 cut(s) 862
BstXI CCANNNNNNTGG 1 cut(s) 1152
BstYI RGATCY 1 cut(s) 862
BsuRI GGCC 1 cut(s) 305
BtgI CCRYGG 1 cut(s) 1092
BtsCI GGATG 1 cut(s) 880
BtsIMutI CAGTG 2 cut(s) 270, 1254
Cac8I GCNNGC 2 cut(s) 971, 1032
CseI GACGC 1 cut(s) 210
Csp6I GTAC 8 cut(s) 367, 437, 491, 514, 703, 1038, 1368, 1469
CspCI CAANNNNNGTGG 2 cut(s) 1243, 1278
CviAII CATG 7 cut(s) 241, 295, 440, 757, 772, 1093, 1336
CviQI GTAC 8 cut(s) 367, 437, 491, 514, 703, 1038, 1368, 1469
DdeI CTNAG 7 cut(s) 350, 627, 672, 889, 956, 974, 1275
DpnI GATC 3 cut(s) 735, 776, 864
DpnII GATC 3 cut(s) 733, 774, 862
DraI TTTAAA 2 cut(s) 142, 345
DrdI GACNNNNNNGTC 1 cut(s) 1232
DseDI GACNNNNNNGTC 1 cut(s) 1232
EaeI YGGCCR 1 cut(s) 303
Eam1104I CTCTTC 2 cut(s) 33, 1377
EarI CTCTTC 2 cut(s) 33, 1377
EciI GGCGGA 2 cut(s) 350, 926
Eco130I CCWWGG 3 cut(s) 300, 1092, 1165
Eco31I GGTCTC 2 cut(s) 25, 193
Eco57I CTGAAG 3 cut(s) 506, 668, 1270
Eco88I CYCGRG 1 cut(s) 1118
EcoNI CCTNNNNNAGG 1 cut(s) 1006
EcoRI GAATTC 1 cut(s) 933
EcoRII CCWGG 1 cut(s) 1132
EcoT14I CCWWGG 3 cut(s) 300, 1092, 1165
ErhI CCWWGG 3 cut(s) 300, 1092, 1165
FaeI CATG 7 cut(s) 244, 298, 443, 760, 775, 1096, 1339
FalI AAGNNNNNCTT 4 cut(s) 641, 673, 1268, 1300
FaqI GGGAC 1 cut(s) 208
FatI CATG 7 cut(s) 240, 294, 439, 756, 771, 1092, 1335
FauI CCCGC 1 cut(s) 249
FbaI TGATCA 2 cut(s) 733, 774
Fnu4HI GCNGC 1 cut(s) 1308
FokI GGATG 1 cut(s) 887
Fsp4HI GCNGC 1 cut(s) 1308
FspBI CTAG 1 cut(s) 813
GluI GCNGC 1 cut(s) 1308
HaeIII GGCC 1 cut(s) 305
HapII CCGG 4 cut(s) 180, 272, 331, 715
HgaI GACGC 1 cut(s) 210
Hin1II CATG 7 cut(s) 244, 298, 443, 760, 775, 1096, 1339
HindIII AAGCTT 2 cut(s) 641, 967
HinfI GANTC 9 cut(s) 244, 260, 268, 373, 445, 466, 623, 667, 1396
HpaII CCGG 4 cut(s) 180, 272, 331, 715
HphI GGTGA 3 cut(s) 15, 538, 1429
Hpy166II GTNNAC 3 cut(s) 369, 769, 1024
Hpy188I TCNGA 5 cut(s) 328, 648, 738, 892, 1317
Hpy188III TCNNGA 7 cut(s) 105, 173, 547, 686, 834, 941, 1118
Hpy8I GTNNAC 3 cut(s) 369, 769, 1024
HpyAV CCTTC 4 cut(s) 692, 831, 865, 1356
HpyCH4III ACNGT 7 cut(s) 68, 102, 116, 417, 1258, 1271, 1332
HpyCH4IV ACGT 3 cut(s) 512, 784, 924
HpyCH4V TGCA 9 cut(s) 45, 196, 412, 554, 578, 662, 782, 1310, 1517
HpyF10VI GCNNNNNNNGC 3 cut(s) 966, 975, 1213
HpyF3I CTNAG 7 cut(s) 350, 627, 672, 889, 956, 974, 1275
HpySE526I ACGT 3 cut(s) 512, 784, 924
Hsp92II CATG 7 cut(s) 244, 298, 443, 760, 775, 1096, 1339
Ksp22I TGATCA 2 cut(s) 733, 774
Kzo9I GATC 3 cut(s) 733, 774, 862
LmnI GCTCC 1 cut(s) 457
Lsp1109I GCAGC 1 cut(s) 1294
LweI GCATC 1 cut(s) 399
MaeI CTAG 1 cut(s) 813
MaeII ACGT 3 cut(s) 512, 784, 924
MaeIII GTNAC 1 cut(s) 1265
MalI GATC 3 cut(s) 735, 776, 864
MbiI CCGCTC 1 cut(s) 256
MboI GATC 3 cut(s) 733, 774, 862
MflI RGATCY 1 cut(s) 862
MlsI TGGCCA 1 cut(s) 305
MluNI TGGCCA 1 cut(s) 305
MlyI GAGTC 1 cut(s) 269
MmeI TCCRAC 2 cut(s) 351, 706
Mox20I TGGCCA 1 cut(s) 305
MroXI GAANNNNTTC 3 cut(s) 653, 933, 1076
MscI TGGCCA 1 cut(s) 305
MseI TTAA 6 cut(s) 141, 344, 963, 1139, 1439, 1525
MslI CAYNNNNRTG 1 cut(s) 1383
Msp20I TGGCCA 1 cut(s) 305
MspI CCGG 4 cut(s) 180, 272, 331, 715
MspR9I CCNGG 3 cut(s) 180, 332, 1134
MvaI CCWGG 1 cut(s) 1134
MvnI CGCG 1 cut(s) 221
MwoI GCNNNNNNNGC 3 cut(s) 966, 975, 1213
NciI CCSGG 2 cut(s) 180, 332
NcoI CCATGG 1 cut(s) 1092
NdeII GATC 3 cut(s) 733, 774, 862
NlaIII CATG 7 cut(s) 244, 298, 443, 760, 775, 1096, 1339
NspV TTCGAA 1 cut(s) 247
OliI CACNNNNGTG 1 cut(s) 1383
PdmI GAANNNNTTC 3 cut(s) 653, 933, 1076
PfeI GAWTC 8 cut(s) 244, 268, 373, 445, 466, 623, 667, 1396
PflFI GACNNNGTC 1 cut(s) 1154
PkrI GCNGC 1 cut(s) 1309
PleI GAGTC 1 cut(s) 268
PpsI GAGTC 1 cut(s) 268
Ppu21I YACGTR 1 cut(s) 513
PshBI ATTAAT 1 cut(s) 1439
PsiI TTATAA 1 cut(s) 1499
Psp6I CCWGG 1 cut(s) 1132
PspGI CCWGG 1 cut(s) 1132
PstI CTGCAG 1 cut(s) 198
PsuI RGATCY 1 cut(s) 862
PsyI GACNNNGTC 1 cut(s) 1154
RsaI GTAC 8 cut(s) 368, 438, 492, 515, 704, 1039, 1369, 1470
RsaNI GTAC 8 cut(s) 367, 437, 491, 514, 703, 1038, 1368, 1469
RseI CAYNNNNRTG 1 cut(s) 1383
SaqAI TTAA 6 cut(s) 141, 344, 963, 1139, 1439, 1525
SatI GCNGC 1 cut(s) 1308
Sau3AI GATC 3 cut(s) 733, 774, 862
SchI GAGTC 1 cut(s) 269
ScrFI CCNGG 3 cut(s) 180, 332, 1134
SfaNI GCATC 1 cut(s) 399
SfcI CTRYAG 2 cut(s) 194, 1176
SfuI TTCGAA 1 cut(s) 247
SmiMI CAYNNNNRTG 1 cut(s) 1383
SmlI CTYRAG 1 cut(s) 278
SmoI CTYRAG 1 cut(s) 278
SsiI CCGC 5 cut(s) 219, 256, 335, 911, 1244
SspI AATATT 2 cut(s) 1065, 1087
SspMI CTAG 1 cut(s) 813
StyD4I CCNGG 3 cut(s) 178, 330, 1132
StyI CCWWGG 3 cut(s) 300, 1092, 1165
TaaI ACNGT 7 cut(s) 68, 102, 116, 417, 1258, 1271, 1332
TaiI ACGT 3 cut(s) 515, 787, 927
TaqI TCGA 2 cut(s) 247, 399
TatI WGTACW 3 cut(s) 366, 490, 702
TfiI GAWTC 8 cut(s) 244, 268, 373, 445, 466, 623, 667, 1396
Tru1I TTAA 6 cut(s) 141, 344, 963, 1139, 1439, 1525
Tru9I TTAA 6 cut(s) 141, 344, 963, 1139, 1439, 1525
TscAI CASTG 2 cut(s) 270, 1261
TseI GCWGC 1 cut(s) 1307
TspDTI ATGAA 4 cut(s) 576, 636, 745, 894
TspGWI ACGGA 1 cut(s) 145
TspRI CASTG 2 cut(s) 270, 1261
Tth111I GACNNNGTC 1 cut(s) 1154
VspI ATTAAT 1 cut(s) 1439
XagI CCTNNNNNAGG 1 cut(s) 1006
XapI RAATTY 4 cut(s) 184, 502, 933, 1501
XcmI CCANNNNNNNNNTGG 1 cut(s) 1099
XmnI GAANNNNTTC 3 cut(s) 653, 933, 1076
XspI CTAG 1 cut(s) 813
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.