MD17G1006300.v1.1

Protein of unknown function (DUF3754)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000718
Physical Location & Seq
Reverse (-)
431796 .. 436267
4472 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1006300.v1.1.491

Sequence Viewer

Length: 1572 bp
ATGGAATTTTTTTTGGTTTATTGTTTGAAGAGAATAAGAAAAAAAAGGTCGTACCCAGTGTACAAGGCTCCCGCTTTACTTAGGGTCTGGGAGAGCCAACGGCCCAACAAGAAGAGGAAATACAAAATCAAAATGGGGAAGAATGAGGTGATTCGGTTAGAGCGTGAATCCGCCATTCAAGTTCTCAAGCCCAAACTCATCATGACCTTGGCTAACCTCATCGAGGAAAGTTCTGATCGTGCTGAGTTTCTCAAGCTCTGCAAGAGAGTCGAGTACACAATTCGGGCTTGGTATCTTCTTCAATTTGAGGATTTGATGCAACTATACTCCCTGTTTGATCCTGTCCATGGGAGTCAGAAATTGGAGCAGCAGAAGCTTTCTCCTCAAGAAGTTGATGTACTTGAACAGAACTTCCTCAAGTACTTGTTTCAGGTGATGGAAAAGAGTAATTTCAAGATTGCAACTAATGAAGAAGTTGAGGTTGCGCAGTCGGGGCAGTATCTTCTAAATCTTCCCATCGTTGTTGATGAATCTAAGCTTGACAATAAACTTTTGAGGAAATACTTTGACAAGCATCCTTGCCCAAACATTCCAGATTTTGCTGATAAGTACGTCATCTTCCGACGTGGCATTGGACTTGATAAGACGGCAGATTACTTTATCATGGAGAAAGTGGACATTATCATTGCACGTTGCTGGAAATATCTTTTACGACTAACCAAGATGGACAAGTTCTTTTCGAAGAAGAAAAAGGCACAGCGCAAGAAGAAGAATATTAGCCTCCTTGACGAAGAGGACTTGTTTGTTGAACGAATCCGTCTTGAAAAAATGGAACTGAGCATTCAAAATTTGCTGTGCAAAAACACAATCCAAGAGCCAACTTTTGATAGGATTATTGTTGTTTACAGGCGAGCAAGTGCCGAAGATAATGCAGAGCGGGGAATATATGTTAAACATTTCAAAAACATTCCAATGGCTGATATGGAGATTGTTCTTCCTGAGAAGAAGAACCCGGGATTAACTCCAATGGATTGGCTCACGTTCGTTGGCTCTGCTGTAGTAGGGCTGGTTGCAGTTATTACTTCGATTGAAATGCCTAAAATTGATGTTTGGGTCATGTTTGCGATTATTTCCACTGTGATTGGTTACTTTGCTAAGACATACTTCAGTTATCAGCAGAACTTGGCTCAGTATCAGAACTTGATAACACAGTCCATGTACGAAAAACAATTGGATAGCGGACGGGGTACTCTCCTTCACTTGTGCGATGATGTGATTCAACAGGAAGTGAAGGAGGTGATAATTGCTTTTTACATACTAATGGAACAAGGGAAAGCTACCGCACTAGATCTGGATAAGTGGTGTGAGGAACTAATCAAAGAACAGTTTAACGACGACTGTAATTTTGATGTGGACGATGCAGTTCAGAAGTTGGAGAAATTGGGAATTGTAACTCGGGATACCCTTGGACGATATAACTGCGTAGGGCTGAAACGTGCGAACGAGATCATTGGCACCACCACAGAGGAGGTTGTTCTTAAGGCAAAACAAGACTTCACGGCCAATGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3754

Protein Analysis

524

Amino Acids

61.21

Weight (kDa)

8.73

Isoelectric Point (pI)

34.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3754 PF12576 295 - 416 6.5e-24 Protein of unknown function (DUF3754)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 486
AccB1I GGYRCC 1 cut(s) 1514
AccBSI CCGCTC 1 cut(s) 937
AciI CCGC 5 cut(s) 72, 171, 937, 1239, 1341
AclWI GGATC 1 cut(s) 332
AcoI YGGCCR 1 cut(s) 1560
AcsI RAATTY 2 cut(s) 5, 847
AcuI CTGAAG 1 cut(s) 1150
AfaI GTAC 8 cut(s) 53, 62, 275, 399, 422, 611, 1220, 1249
AfiI CCNNNNNNNGG 2 cut(s) 223, 347
AflII CTTAAG 1 cut(s) 1538
AjiI CACGTC 1 cut(s) 626
AloI GAACNNNNNNTCC 2 cut(s) 396, 428
AluBI AGCT 4 cut(s) 256, 376, 538, 1337
AluI AGCT 4 cut(s) 256, 376, 538, 1337
AlwI GGATC 1 cut(s) 332
Ama87I CYCGRG 2 cut(s) 1012, 1455
AoxI GGCC 2 cut(s) 101, 1560
ApeKI GCWGC 1 cut(s) 367
ApoI RAATTY 2 cut(s) 5, 847
ArsI GACNNNNNNTTYG 2 cut(s) 1388, 1420
AspLEI GCGC 2 cut(s) 487, 762
AspS9I GGNCC 1 cut(s) 102
AsuC2I CCSGG 2 cut(s) 1013, 1014
AsuHPI GGTGA 3 cut(s) 160, 445, 1309
AsuII TTCGAA 1 cut(s) 740
AvaI CYCGRG 2 cut(s) 1012, 1455
BanI GGYRCC 1 cut(s) 1514
BarI GAAGNNNNNNTAC 6 cut(s) 104, 136, 381, 413, 602, 634
BbvI GCAGC 1 cut(s) 379
BccI CCATC 3 cut(s) 430, 524, 718
BceAI ACGGC 2 cut(s) 116, 663
BcgI CGANNNNNNTGC 8 cut(s) 250, 284, 911, 945, 1075, 1109, 1461, 1495
BciVI GTATCC 1 cut(s) 1453
BcnI CCSGG 2 cut(s) 1013, 1014
BfaI CTAG 1 cut(s) 1346
BfmI CTRYAG 1 cut(s) 1056
BfrI CTTAAG 1 cut(s) 1538
BfuI GTATCC 1 cut(s) 1453
BglII AGATCT 1 cut(s) 1348
BisI GCNGC 1 cut(s) 368
BlsI GCNGC 1 cut(s) 369
BmcAI AGTACT 1 cut(s) 422
Bme1390I CCNGG 2 cut(s) 1013, 1014
BmeT110I CYCGRG 2 cut(s) 1012, 1455
BmgBI CACGTC 1 cut(s) 626
BmgT120I GGNCC 1 cut(s) 102
BmiI GGNNCC 2 cut(s) 69, 1516
BmrFI CCNGG 2 cut(s) 1013, 1014
BmrI ACTGGG 1 cut(s) 50
BmsI GCATC 3 cut(s) 306, 583, 1408
BmuI ACTGGG 1 cut(s) 50
Bpu14I TTCGAA 1 cut(s) 740
BpuEI CTTGAG 4 cut(s) 170, 236, 369, 401
BpuMI CCSGG 2 cut(s) 1013, 1014
BsaJI CCNNGG 4 cut(s) 207, 346, 1012, 1465
Bsc4I CCNNNNNNNGG 2 cut(s) 223, 347
Bse1I ACTGG 1 cut(s) 56
Bse3DI GCAATG 1 cut(s) 684
BseDI CCNNGG 4 cut(s) 207, 346, 1012, 1465
BseGI GGATG 1 cut(s) 574
BseLI CCNNNNNNNGG 2 cut(s) 223, 347
BseMI GCAATG 1 cut(s) 684
BseMII CTCAG 4 cut(s) 234, 827, 990, 1202
BseNI ACTGG 1 cut(s) 56
BseRI GAGGAG 2 cut(s) 372, 1541
BseXI GCAGC 1 cut(s) 379
BshFI GGCC 2 cut(s) 103, 1562
BshNI GGYRCC 1 cut(s) 1514
BsiHKCI CYCGRG 2 cut(s) 1012, 1455
BsiSI CCGG 1 cut(s) 1013
BslI CCNNNNNNNGG 2 cut(s) 223, 347
BsmI GAATGC 1 cut(s) 840
BsnI GGCC 2 cut(s) 103, 1562
BsoBI CYCGRG 2 cut(s) 1012, 1455
Bsp119I TTCGAA 1 cut(s) 740
Bsp1407I TGTACA 1 cut(s) 60
Bsp143I GATC 4 cut(s) 235, 337, 1348, 1506
Bsp19I CCATGG 1 cut(s) 346
BspACI CCGC 5 cut(s) 72, 171, 937, 1239, 1341
BspANI GGCC 2 cut(s) 103, 1562
BspCNI CTCAG 4 cut(s) 235, 828, 991, 1201
BspHI TCATGA 1 cut(s) 201
BspLI GGNNCC 2 cut(s) 69, 1516
BspPI GGATC 1 cut(s) 332
BspT104I TTCGAA 1 cut(s) 740
BspT107I GGYRCC 1 cut(s) 1514
BspTI CTTAAG 1 cut(s) 1538
BsrBI CCGCTC 1 cut(s) 937
BsrDI GCAATG 1 cut(s) 684
BsrGI TGTACA 1 cut(s) 60
BsrI ACTGG 1 cut(s) 56
BssECI CCNNGG 4 cut(s) 207, 346, 1012, 1465
BssMI GATC 4 cut(s) 235, 337, 1348, 1506
BssT1I CCWWGG 3 cut(s) 207, 346, 1465
Bst4CI ACNGT 4 cut(s) 1138, 1212, 1386, 1400
Bst6I CTCTTC 3 cut(s) 23, 107, 786
BstAFI CTTAAG 1 cut(s) 1538
BstAUI TGTACA 1 cut(s) 60
BstBI TTCGAA 1 cut(s) 740
BstC8I GCNNGC 1 cut(s) 912
BstDEI CTNAG 7 cut(s) 80, 243, 534, 836, 999, 1155, 1188
BstDSI CCRYGG 1 cut(s) 346
BstENI CCTNNNNNAGG 1 cut(s) 221
BstF5I GGATG 1 cut(s) 574
BstHHI GCGC 2 cut(s) 487, 762
BstKTI GATC 4 cut(s) 238, 340, 1351, 1509
BstMBI GATC 4 cut(s) 235, 337, 1348, 1506
BstMWI GCNNNNNNNGC 2 cut(s) 373, 493
BstSCI CCNGG 2 cut(s) 1011, 1012
BstSFI CTRYAG 1 cut(s) 1056
BstV1I GCAGC 1 cut(s) 379
BstX2I RGATCY 1 cut(s) 1348
BstXI CCANNNNNNTGG 1 cut(s) 1032
BstYI RGATCY 1 cut(s) 1348
BsuI GTATCC 1 cut(s) 1453
BsuRI GGCC 2 cut(s) 103, 1562
BtgI CCRYGG 1 cut(s) 346
BtgZI GCGATG 1 cut(s) 1281
BtrI CACGTC 1 cut(s) 626
BtsCI GGATG 1 cut(s) 574
BtsIMutI CAGTG 2 cut(s) 63, 1134
Cac8I GCNNGC 1 cut(s) 912
CciI TCATGA 1 cut(s) 201
CfoI GCGC 2 cut(s) 487, 762
Cfr13I GGNCC 1 cut(s) 102
Cfr9I CCCGGG 1 cut(s) 1012
Csp6I GTAC 8 cut(s) 52, 61, 274, 398, 421, 610, 1219, 1248
CspCI CAANNNNNGTGG 2 cut(s) 1123, 1158
CviAII CATG 5 cut(s) 202, 347, 664, 1117, 1216
CviQI GTAC 8 cut(s) 52, 61, 274, 398, 421, 610, 1219, 1248
DdeI CTNAG 7 cut(s) 80, 243, 534, 836, 999, 1155, 1188
DpnI GATC 4 cut(s) 237, 339, 1350, 1508
DpnII GATC 4 cut(s) 235, 337, 1348, 1506
EaeI YGGCCR 1 cut(s) 1560
Eam1104I CTCTTC 3 cut(s) 23, 107, 786
EarI CTCTTC 3 cut(s) 23, 107, 786
EciI GGCGGA 1 cut(s) 160
Eco130I CCWWGG 3 cut(s) 207, 346, 1465
Eco57I CTGAAG 1 cut(s) 1150
Eco88I CYCGRG 2 cut(s) 1012, 1455
EcoNI CCTNNNNNAGG 1 cut(s) 221
EcoT14I CCWWGG 3 cut(s) 207, 346, 1465
ErhI CCWWGG 3 cut(s) 207, 346, 1465
FaeI CATG 5 cut(s) 205, 350, 667, 1120, 1219
FalI AAGNNNNNCTT 2 cut(s) 1148, 1180
FatI CATG 5 cut(s) 201, 346, 663, 1116, 1215
FauI CCCGC 2 cut(s) 79, 930
Fnu4HI GCNGC 1 cut(s) 368
FokI GGATG 1 cut(s) 561
Fsp4HI GCNGC 1 cut(s) 368
FspBI CTAG 1 cut(s) 1346
FspI TGCGCA 1 cut(s) 486
GlaI GCGC 2 cut(s) 486, 761
GluI GCNGC 1 cut(s) 368
HaeIII GGCC 2 cut(s) 103, 1562
HapII CCGG 1 cut(s) 1013
HhaI GCGC 2 cut(s) 487, 762
Hin1II CATG 5 cut(s) 205, 350, 667, 1120, 1219
Hin6I GCGC 2 cut(s) 485, 760
HinP1I GCGC 2 cut(s) 485, 760
HindIII AAGCTT 2 cut(s) 374, 536
HinfI GANTC 7 cut(s) 151, 167, 267, 352, 530, 813, 1276
HpaII CCGG 1 cut(s) 1013
HphI GGTGA 3 cut(s) 160, 445, 1309
Hpy166II GTNNAC 5 cut(s) 61, 276, 676, 904, 1414
Hpy188I TCNGA 5 cut(s) 235, 357, 623, 1197, 1428
Hpy188III TCNNGA 8 cut(s) 202, 386, 454, 593, 821, 998, 1352, 1457
Hpy8I GTNNAC 5 cut(s) 61, 276, 676, 904, 1414
Hpy99I CGWCG 2 cut(s) 627, 1397
HpyAV CCTTC 2 cut(s) 1265, 1285
HpyCH4III ACNGT 4 cut(s) 1138, 1212, 1386, 1400
HpyCH4IV ACGT 5 cut(s) 612, 625, 691, 1040, 1495
HpyCH4V TGCA 8 cut(s) 261, 319, 461, 689, 858, 932, 1073, 1421
HpyF10VI GCNNNNNNNGC 2 cut(s) 373, 493
HpyF3I CTNAG 7 cut(s) 80, 243, 534, 836, 999, 1155, 1188
HpySE526I ACGT 5 cut(s) 612, 625, 691, 1040, 1495
Hsp92II CATG 5 cut(s) 205, 350, 667, 1120, 1219
HspAI GCGC 2 cut(s) 485, 760
Kzo9I GATC 4 cut(s) 235, 337, 1348, 1506
LmnI GCTCC 2 cut(s) 73, 364
Lsp1109I GCAGC 1 cut(s) 379
LweI GCATC 3 cut(s) 306, 583, 1408
MaeI CTAG 1 cut(s) 1346
MaeII ACGT 5 cut(s) 612, 625, 691, 1040, 1495
MaeIII GTNAC 2 cut(s) 1145, 1450
MalI GATC 4 cut(s) 237, 339, 1350, 1508
MbiI CCGCTC 1 cut(s) 937
MboI GATC 4 cut(s) 235, 337, 1348, 1506
MfeI CAATTG 1 cut(s) 1229
MflI RGATCY 1 cut(s) 1348
MlyI GAGTC 2 cut(s) 276, 361
MmeI TCCRAC 2 cut(s) 646, 1413
MseI TTAA 4 cut(s) 951, 1019, 1389, 1539
MslI CAYNNNNRTG 2 cut(s) 971, 1319
MspCI CTTAAG 1 cut(s) 1538
MspI CCGG 1 cut(s) 1013
MspR9I CCNGG 2 cut(s) 1013, 1014
MunI CAATTG 1 cut(s) 1229
Mva1269I GAATGC 1 cut(s) 840
MwoI GCNNNNNNNGC 2 cut(s) 373, 493
NciI CCSGG 2 cut(s) 1013, 1014
NcoI CCATGG 1 cut(s) 346
NdeII GATC 4 cut(s) 235, 337, 1348, 1506
NlaIII CATG 5 cut(s) 205, 350, 667, 1120, 1219
NlaIV GGNNCC 2 cut(s) 69, 1516
NsbI TGCGCA 1 cut(s) 486
NspV TTCGAA 1 cut(s) 740
PagI TCATGA 1 cut(s) 201
PcsI WCGNNNNNNNCGW 1 cut(s) 160
PctI GAATGC 1 cut(s) 840
PfeI GAWTC 5 cut(s) 151, 167, 530, 813, 1276
PkrI GCNGC 1 cut(s) 369
PleI GAGTC 2 cut(s) 275, 360
PpsI GAGTC 2 cut(s) 275, 360
PspN4I GGNNCC 2 cut(s) 69, 1516
PspPI GGNCC 1 cut(s) 102
PsuI RGATCY 1 cut(s) 1348
RsaI GTAC 8 cut(s) 53, 62, 275, 399, 422, 611, 1220, 1249
RsaNI GTAC 8 cut(s) 52, 61, 274, 398, 421, 610, 1219, 1248
RseI CAYNNNNRTG 2 cut(s) 971, 1319
SaqAI TTAA 4 cut(s) 951, 1019, 1389, 1539
SatI GCNGC 1 cut(s) 368
Sau3AI GATC 4 cut(s) 235, 337, 1348, 1506
Sau96I GGNCC 1 cut(s) 102
ScaI AGTACT 1 cut(s) 422
SchI GAGTC 2 cut(s) 276, 361
ScrFI CCNGG 2 cut(s) 1013, 1014
SfaNI GCATC 3 cut(s) 306, 583, 1408
SfcI CTRYAG 1 cut(s) 1056
SfuI TTCGAA 1 cut(s) 740
SmaI CCCGGG 1 cut(s) 1014
SmiMI CAYNNNNRTG 2 cut(s) 971, 1319
SmlI CTYRAG 5 cut(s) 185, 251, 384, 416, 1538
SmoI CTYRAG 5 cut(s) 185, 251, 384, 416, 1538
SsiI CCGC 5 cut(s) 72, 171, 937, 1239, 1341
SspI AATATT 1 cut(s) 775
SspMI CTAG 1 cut(s) 1346
StyD4I CCNGG 2 cut(s) 1011, 1012
StyI CCWWGG 3 cut(s) 207, 346, 1465
TaaI ACNGT 4 cut(s) 1138, 1212, 1386, 1400
TaiI ACGT 5 cut(s) 615, 628, 694, 1043, 1498
TaqI TCGA 4 cut(s) 222, 270, 740, 1085
TatI WGTACW 4 cut(s) 60, 273, 397, 420
TfiI GAWTC 5 cut(s) 151, 167, 530, 813, 1276
Tru1I TTAA 4 cut(s) 951, 1019, 1389, 1539
Tru9I TTAA 4 cut(s) 951, 1019, 1389, 1539
TscAI CASTG 2 cut(s) 63, 1141
TseI GCWGC 1 cut(s) 367
TspDTI ATGAA 2 cut(s) 483, 543
TspGWI ACGGA 1 cut(s) 806
TspMI CCCGGG 1 cut(s) 1012
TspRI CASTG 2 cut(s) 63, 1141
Vha464I CTTAAG 1 cut(s) 1538
XagI CCTNNNNNAGG 1 cut(s) 221
XapI RAATTY 2 cut(s) 5, 847
XmaI CCCGGG 1 cut(s) 1012
XspI CTAG 1 cut(s) 1346
ZrmI AGTACT 1 cut(s) 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.