Rorug02G0552900

Protein of unknown function (DUF3754)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000718
Physical Location & Seq
Forward (+)
68000594 .. 68001365
772 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0552900.1

Sequence Viewer

Length: 696 bp
ATGGCCGCTTCCTCCTACCCGGAAACCCCCCAAATGTCAAGAAAGTCCAAGAACACGTACACGTGGAAGGGTTTCGGCAAGGTCCCTAACGCCCTGAAGGAGCTCAGAGAAGAAGGCCTGGATCTGACTCCGATCCCGGTGGCCTGCAATAACAACAACAACAATCCCCTGGATGAGACCAAGAGGGAAAAGTCTCTGGCGCTTCTGGCGCAGAATTTCGTGAAGCTGTTCGTGTGTACCGGATCGGAATCGGAATCGATTTCTCTTGATGAAGCTGCGAAATTACTGCTTGGAGTAGGGGATGCACAAAATGTATCAGTGATGAGGACGAAGGGAAGCCCGTACAGGTGGTTGGGGATGAATGGGAGAAGAGATGGTTTGAATTTGAATTCGGAGCCGAATTTGGAGCAGGCTAAGAAGAGGAGCTTTGAGACTGATCTGACGAATGCGAGTAGTAAGAGGAGCAAGGTTGTGGAGAAGAGAGTTCAAGATCAGGGTGAGGTTGAGAACTGCAATTCGGATGGCTGTGGGGCGAAGGAGAGTTCGAAGAGCTACCAGTTTGGTCCTTTCGCTCTAGTGGCGGTTCCAAGAGCTAGGAAGGGTGTTGACATCAACAAGGTTTGCTGGGAGTCTTGGGGCTTATTATCGGCCTCAATATCAGAACCAAGCGATGAAGGAGCTGTTTTCACATTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3754

Protein Analysis

231

Amino Acids

25.31

Weight (kDa)

8.21

Isoelectric Point (pI)

44.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 6, 581
AclWI GGATC 3 cut(s) 127, 129, 250
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 4 cut(s) 214, 382, 388, 400
AcuI CTGAAG 1 cut(s) 116
AcvI CACGTG 1 cut(s) 63
AfaI GTAC 3 cut(s) 59, 238, 344
AflIII ACRYGT 2 cut(s) 54, 60
AgsI TTSAA 3 cut(s) 382, 388, 488
AjnI CCWGG 2 cut(s) 117, 168
AluBI AGCT 7 cut(s) 103, 226, 275, 426, 552, 593, 680
AluI AGCT 7 cut(s) 103, 226, 275, 426, 552, 593, 680
Alw21I GWGCWC 1 cut(s) 105
Alw26I GTCTC 3 cut(s) 170, 198, 425
AlwI GGATC 3 cut(s) 127, 129, 250
AoxI GGCC 4 cut(s) 3, 115, 141, 648
ApeKI GCWGC 1 cut(s) 275
ApoI RAATTY 4 cut(s) 214, 382, 388, 400
Asp700I GAANNNNTTC 2 cut(s) 71, 227
AspLEI GCGC 2 cut(s) 202, 211
AspS9I GGNCC 2 cut(s) 82, 563
AsuC2I CCSGG 2 cut(s) 20, 137
AsuHPI GGTGA 1 cut(s) 509
AsuII TTCGAA 1 cut(s) 545
AvaII GGWCC 2 cut(s) 82, 563
BanII GRGCYC 1 cut(s) 105
BbrPI CACGTG 1 cut(s) 63
Bbv12I GWGCWC 1 cut(s) 105
BbvI GCAGC 1 cut(s) 262
BccI CCATC 2 cut(s) 368, 515
BcgI CGANNNNNNTGC 2 cut(s) 268, 302
BciT130I CCWGG 2 cut(s) 119, 170
BcnI CCSGG 2 cut(s) 20, 137
BcoDI GTCTC 3 cut(s) 170, 198, 425
BfaI CTAG 2 cut(s) 575, 594
BfoI RGCGCY 1 cut(s) 203
BisI GCNGC 2 cut(s) 6, 276
BlsI GCNGC 2 cut(s) 7, 277
Bme1390I CCNGG 4 cut(s) 20, 119, 137, 170
Bme18I GGWCC 2 cut(s) 82, 563
BmgT120I GGNCC 2 cut(s) 82, 563
BmiI GGNNCC 3 cut(s) 84, 396, 585
BmrFI CCNGG 4 cut(s) 20, 119, 137, 170
BmsI GCATC 1 cut(s) 292
Bpu14I TTCGAA 1 cut(s) 545
BpuMI CCSGG 2 cut(s) 20, 137
Bsa29I ATCGAT 1 cut(s) 257
BsaAI YACGTR 2 cut(s) 57, 63
BsaBI GATNNNNATC 1 cut(s) 247
BsaI GGTCTC 1 cut(s) 170
BsaJI CCNNGG 1 cut(s) 168
BsaWI WCCGGW 1 cut(s) 239
Bse1I ACTGG 1 cut(s) 556
Bse8I GATNNNNATC 1 cut(s) 247
BseBI CCWGG 2 cut(s) 119, 170
BseCI ATCGAT 1 cut(s) 257
BseDI CCNNGG 1 cut(s) 168
BseGI GGATG 4 cut(s) 178, 307, 363, 526
BseJI GATNNNNATC 1 cut(s) 247
BseMII CTCAG 1 cut(s) 118
BseNI ACTGG 1 cut(s) 556
BseRI GAGGAG 2 cut(s) 436, 475
BseXI GCAGC 1 cut(s) 262
BseYI CCCAGC 1 cut(s) 624
BshFI GGCC 4 cut(s) 5, 117, 143, 650
BshVI ATCGAT 1 cut(s) 257
BsiHKAI GWGCWC 1 cut(s) 105
BsiSI CCGG 3 cut(s) 20, 137, 240
BslFI GGGAC 1 cut(s) 68
BsmAI GTCTC 3 cut(s) 170, 198, 425
BsmFI GGGAC 1 cut(s) 68
BsmI GAATGC 1 cut(s) 451
BsnI GGCC 4 cut(s) 5, 117, 143, 650
Bso31I GGTCTC 1 cut(s) 170
Bsp119I TTCGAA 1 cut(s) 545
Bsp1286I GDGCHC 1 cut(s) 105
Bsp143I GATC 5 cut(s) 121, 132, 242, 436, 490
BspACI CCGC 2 cut(s) 6, 581
BspANI GGCC 4 cut(s) 5, 117, 143, 650
BspCNI CTCAG 1 cut(s) 117
BspDI ATCGAT 1 cut(s) 257
BspLI GGNNCC 3 cut(s) 84, 396, 585
BspPI GGATC 3 cut(s) 127, 129, 250
BspQI GCTCTTC 1 cut(s) 542
BspT104I TTCGAA 1 cut(s) 545
BspTNI GGTCTC 1 cut(s) 170
BsrI ACTGG 1 cut(s) 556
BssECI CCNNGG 1 cut(s) 168
BssMI GATC 5 cut(s) 121, 132, 242, 436, 490
Bst2UI CCWGG 2 cut(s) 119, 170
Bst6I CTCTTC 4 cut(s) 364, 413, 473, 542
BstBAI YACGTR 2 cut(s) 57, 63
BstBI TTCGAA 1 cut(s) 545
BstC8I GCNNGC 2 cut(s) 145, 411
BstDEI CTNAG 2 cut(s) 104, 414
BstF5I GGATG 4 cut(s) 178, 307, 363, 526
BstH2I RGCGCY 1 cut(s) 203
BstHHI GCGC 2 cut(s) 202, 211
BstKTI GATC 5 cut(s) 124, 135, 245, 439, 493
BstMAI GTCTC 3 cut(s) 170, 198, 425
BstMBI GATC 5 cut(s) 121, 132, 242, 436, 490
BstMWI GCNNNNNNNGC 3 cut(s) 206, 208, 578
BstNI CCWGG 2 cut(s) 119, 170
BstSCI CCNGG 4 cut(s) 18, 117, 135, 168
BstV1I GCAGC 1 cut(s) 262
BstX2I RGATCY 1 cut(s) 121
BstYI RGATCY 1 cut(s) 121
Bsu15I ATCGAT 1 cut(s) 257
BsuRI GGCC 4 cut(s) 5, 117, 143, 650
BsuTUI ATCGAT 1 cut(s) 257
BtgZI GCGATG 1 cut(s) 684
BtsCI GGATG 4 cut(s) 178, 307, 363, 526
BtsIMutI CAGTG 1 cut(s) 324
Cac8I GCNNGC 2 cut(s) 145, 411
CfoI GCGC 2 cut(s) 202, 211
Cfr13I GGNCC 2 cut(s) 82, 563
ClaI ATCGAT 1 cut(s) 257
Csp6I GTAC 3 cut(s) 58, 237, 343
CviQI GTAC 3 cut(s) 58, 237, 343
DdeI CTNAG 2 cut(s) 104, 414
DpnI GATC 5 cut(s) 123, 134, 244, 438, 492
DpnII GATC 5 cut(s) 121, 132, 242, 436, 490
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 4 cut(s) 364, 413, 473, 542
EarI CTCTTC 4 cut(s) 364, 413, 473, 542
Ecl136II GAGCTC 1 cut(s) 103
Eco147I AGGCCT 1 cut(s) 117
Eco24I GRGCYC 1 cut(s) 105
Eco31I GGTCTC 1 cut(s) 170
Eco47I GGWCC 2 cut(s) 82, 563
Eco53kI GAGCTC 1 cut(s) 103
Eco57I CTGAAG 1 cut(s) 116
Eco72I CACGTG 1 cut(s) 63
EcoICRI GAGCTC 1 cut(s) 103
EcoO109I RGGNCCY 1 cut(s) 82
EcoRI GAATTC 1 cut(s) 388
EcoRII CCWGG 2 cut(s) 117, 168
EcoT38I GRGCYC 1 cut(s) 105
FaiI YATR 1 cut(s) 694
FalI AAGNNNNNCTT 2 cut(s) 410, 442
FaqI GGGAC 1 cut(s) 68
Fnu4HI GCNGC 2 cut(s) 6, 276
FokI GGATG 4 cut(s) 185, 314, 370, 533
FriOI GRGCYC 1 cut(s) 105
Fsp4HI GCNGC 2 cut(s) 6, 276
FspBI CTAG 2 cut(s) 575, 594
GlaI GCGC 2 cut(s) 201, 210
GluI GCNGC 2 cut(s) 6, 276
GsaI CCCAGC 1 cut(s) 628
HaeII RGCGCY 1 cut(s) 203
HaeIII GGCC 4 cut(s) 5, 117, 143, 650
HapII CCGG 3 cut(s) 20, 137, 240
HhaI GCGC 2 cut(s) 202, 211
Hin6I GCGC 2 cut(s) 200, 209
HinP1I GCGC 2 cut(s) 200, 209
HincII GTYRAC 1 cut(s) 607
HindII GTYRAC 1 cut(s) 607
HinfI GANTC 4 cut(s) 127, 248, 254, 629
HpaII CCGG 3 cut(s) 20, 137, 240
HphI GGTGA 1 cut(s) 509
Hpy166II GTNNAC 3 cut(s) 60, 237, 607
Hpy188I TCNGA 9 cut(s) 107, 126, 132, 247, 253, 394, 441, 520, 661
Hpy188III TCNNGA 4 cut(s) 39, 220, 266, 488
Hpy8I GTNNAC 3 cut(s) 60, 237, 607
HpyAV CCTTC 7 cut(s) 61, 91, 107, 325, 529, 592, 668
HpyCH4IV ACGT 2 cut(s) 56, 62
HpyCH4V TGCA 3 cut(s) 147, 305, 513
HpyF10VI GCNNNNNNNGC 3 cut(s) 206, 208, 578
HpyF3I CTNAG 2 cut(s) 104, 414
HpySE526I ACGT 2 cut(s) 56, 62
HspAI GCGC 2 cut(s) 200, 209
Kzo9I GATC 5 cut(s) 121, 132, 242, 436, 490
LguI GCTCTTC 1 cut(s) 542
LmnI GCTCC 6 cut(s) 100, 394, 406, 423, 462, 677
Lsp1109I GCAGC 1 cut(s) 262
LweI GCATC 1 cut(s) 292
MaeI CTAG 2 cut(s) 575, 594
MaeII ACGT 2 cut(s) 56, 62
MalI GATC 5 cut(s) 123, 134, 244, 438, 492
MboI GATC 5 cut(s) 121, 132, 242, 436, 490
MboII GAAGA 5 cut(s) 122, 381, 430, 490, 559
MflI RGATCY 1 cut(s) 121
MhlI GDGCHC 1 cut(s) 105
MluCI AATT 6 cut(s) 214, 281, 382, 388, 400, 514
MlyI GAGTC 2 cut(s) 121, 638
MnlI CCTC 7 cut(s) 22, 177, 318, 414, 453, 493, 661
MroXI GAANNNNTTC 2 cut(s) 71, 227
MspI CCGG 3 cut(s) 20, 137, 240
MspR9I CCNGG 4 cut(s) 20, 119, 137, 170
Mva1269I GAATGC 1 cut(s) 451
MvaI CCWGG 2 cut(s) 119, 170
MwoI GCNNNNNNNGC 3 cut(s) 206, 208, 578
NciI CCSGG 2 cut(s) 20, 137
NdeII GATC 5 cut(s) 121, 132, 242, 436, 490
NlaIV GGNNCC 3 cut(s) 84, 396, 585
NspV TTCGAA 1 cut(s) 545
PceI AGGCCT 1 cut(s) 117
PciSI GCTCTTC 1 cut(s) 542
PctI GAATGC 1 cut(s) 451
PdmI GAANNNNTTC 2 cut(s) 71, 227
PfeI GAWTC 2 cut(s) 248, 254
PkrI GCNGC 2 cut(s) 7, 277
PleI GAGTC 2 cut(s) 121, 637
PmaCI CACGTG 1 cut(s) 63
PmlI CACGTG 1 cut(s) 63
PpsI GAGTC 2 cut(s) 121, 637
Ppu21I YACGTR 2 cut(s) 57, 63
PpuMI RGGWCCY 1 cut(s) 82
Psp124BI GAGCTC 1 cut(s) 105
Psp5II RGGWCCY 1 cut(s) 82
Psp6I CCWGG 2 cut(s) 117, 168
PspCI CACGTG 1 cut(s) 63
PspFI CCCAGC 1 cut(s) 624
PspGI CCWGG 2 cut(s) 117, 168
PspN4I GGNNCC 3 cut(s) 84, 396, 585
PspPI GGNCC 2 cut(s) 82, 563
PspPPI RGGWCCY 1 cut(s) 82
PsuI RGATCY 1 cut(s) 121
RsaI GTAC 3 cut(s) 59, 238, 344
RsaNI GTAC 3 cut(s) 58, 237, 343
SacI GAGCTC 1 cut(s) 105
SapI GCTCTTC 1 cut(s) 542
SatI GCNGC 2 cut(s) 6, 276
Sau3AI GATC 5 cut(s) 121, 132, 242, 436, 490
Sau96I GGNCC 2 cut(s) 82, 563
SchI GAGTC 2 cut(s) 121, 638
ScrFI CCNGG 4 cut(s) 20, 119, 137, 170
SduI GDGCHC 1 cut(s) 105
SfaNI GCATC 1 cut(s) 292
SfuI TTCGAA 1 cut(s) 545
SinI GGWCC 2 cut(s) 82, 563
Sse9I AATT 6 cut(s) 214, 281, 382, 388, 400, 514
SseBI AGGCCT 1 cut(s) 117
SsiI CCGC 2 cut(s) 6, 581
SspMI CTAG 2 cut(s) 575, 594
SstI GAGCTC 1 cut(s) 105
StuI AGGCCT 1 cut(s) 117
StyD4I CCNGG 4 cut(s) 18, 117, 135, 168
TaiI ACGT 2 cut(s) 59, 65
TaqI TCGA 2 cut(s) 257, 545
TasI AATT 6 cut(s) 214, 281, 382, 388, 400, 514
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 2 cut(s) 248, 254
TscAI CASTG 1 cut(s) 324
TseI GCWGC 1 cut(s) 275
TspDTI ATGAA 3 cut(s) 285, 374, 687
TspRI CASTG 1 cut(s) 324
VpaK11BI GGWCC 2 cut(s) 82, 563
XapI RAATTY 4 cut(s) 214, 382, 388, 400
XmnI GAANNNNTTC 2 cut(s) 71, 227
XspI CTAG 2 cut(s) 575, 594
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.