Rmu_sc0007623.1_g000005

Protein of unknown function (DUF3754)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0007623.1
OG0000718
Physical Location & Seq
Forward (+)
27782 .. 33387
5606 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0007623.1_g000005.1.cds

Sequence Viewer

Length: 1680 bp
atggaaaaaaccgcgtcccagaagaagaaagacatgattcgaatagagcgcgagtccgtgattccggttctcaagcccaagctcatcatgtccttggccaacctcattgaacatagttccgaccgggcggagtttttaaagctctgcaagagaattgagtacacgattcgggcttggtatctgctacaattcgaggatttgatgcaactgtattcgctctttgaccccgtccatgggaatcaaaaattggagcaacagaatctttctcctaaagagattgatgtactggaacagaatttcctcacgtacttacttcaggtgatggaaaagagtaatttcaagattgcaactgatgaagaaattgagattgcacattcagggcagtatcttctaaatcttcccatcgtggttgatgaatctaagattgacaagaagcttctgaagaacttctttgcagagaatcctcagccaaaccttccagattttgctgataagtacattatcttccggcgtggtgttggacttgatcagacaactgattactttttcatggagaaagtggacatgatcattgcacgtttttgggcatatcttttacgaataactagggctgaaaaacttctatccagaaggtcaagtagtcggcacacaaaagatccaaagaaggatgatgaaattacctcagaagcagatggggaggaggcggaggacttatacgttgaacgaattcgtcttgaaaatatggaactaagcgttaaaagcttgctgagcaagaccacaatcaaagagccaacctttgataggataattgttgtttacaggcgagcaagtaccaaagcaaaagaagaacgaggaatatttgtgaaacatttcaaaaatattcccatggctgatatggagatagttcttcccgagaagaagaacccaggattaactccaatggactgggtcaagttccttggctctgctgtagttgggctggttgctgttattagttcgcttgaaatgcctaatgctgacttttgggtcatcattgcggttctttccactgtcattggttactgtgctaagatatacttcagttttcagcagaacttggctgcatatcagaacctaataacacagtccatgtatgacaagcaactggatagcggaaggggtactcttcttcacttgtgtgatgatgtgattcaacaggaggtcaaggaggtgatactttctttctatatattaatggaacagggaaaagctacaagagaagatctggataggtggtgtgaggaactcattaaagaagagtttggtgcgagttgtaattttgatgtggatgatgcagttgggaagttggagaaattgggaattgtctctcgggattccgttggacggtatttctgcgttgggctgaaacgtgcaaatgaaatacttgggaccaccactgaggagcttgttatgagggcaaaacaaggcaaacaaggcagtgctagaccatcatcaatcctctttgatggaagattatcctcaaatatttcgctgcttagagagaagtcatcctccaccttgatgtgctccggtgacaagtggccggcgcggtctccggcgaccggcgaccggactctggcgaacggtgaccgggttccggcgaccggtgaccggaatccggctattattgccccccagtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3754

Protein Analysis

559

Amino Acids

63.81

Weight (kDa)

5.93

Isoelectric Point (pI)

40.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1025
AccII CGCG 3 cut(s) 14, 51, 1588
AciI CCGC 6 cut(s) 12, 128, 704, 1037, 1152, 1588
AclWI GGATC 1 cut(s) 650
AcoI YGGCCR 2 cut(s) 96, 1580
AcsI RAATTY 2 cut(s) 295, 726
AcuI CTGAAG 3 cut(s) 299, 461, 1063
AfaI GTAC 6 cut(s) 161, 285, 308, 497, 832, 1162
AgeI ACCGGT 1 cut(s) 1643
AgsI TTSAA 7 cut(s) 110, 340, 722, 737, 874, 1004, 1193
AjnI CCWGG 1 cut(s) 925
AjuI GAANNNNNNNTTGG 2 cut(s) 230, 262
AleI CACNNNNGTG 1 cut(s) 1176
AloI GAACNNNNNNTCC 2 cut(s) 282, 314
AluBI AGCT 6 cut(s) 82, 142, 436, 762, 1250, 1444
AluI AGCT 6 cut(s) 82, 142, 436, 762, 1250, 1444
Alw21I GWGCWC 1 cut(s) 1568
Alw26I GTCTC 2 cut(s) 1369, 1596
AlwI GGATC 1 cut(s) 650
Ama87I CYCGRG 2 cut(s) 911, 1368
AoxI GGCC 2 cut(s) 96, 1580
ApeKI GCWGC 2 cut(s) 1100, 1531
ApoI RAATTY 2 cut(s) 295, 726
AseI ATTAAT 1 cut(s) 1232
AsiGI ACCGGT 1 cut(s) 1643
Asp700I GAANNNNTTC 4 cut(s) 446, 618, 726, 869
AspLEI GCGC 2 cut(s) 51, 1588
AspS9I GGNCC 1 cut(s) 1428
AsuC2I CCSGG 2 cut(s) 125, 1631
AsuHPI GGTGA 5 cut(s) 331, 1222, 1583, 1637, 1658
AsuII TTCGAA 1 cut(s) 40
AvaI CYCGRG 2 cut(s) 911, 1368
AvaII GGWCC 1 cut(s) 1428
BalI TGGCCA 1 cut(s) 98
BarI GAAGNNNNNNTAC 2 cut(s) 488, 520
Bbv12I GWGCWC 1 cut(s) 1568
BbvCI CCTCAGC 1 cut(s) 465
BbvI GCAGC 2 cut(s) 1087, 1518
BccI CCATC 5 cut(s) 316, 410, 686, 1495, 1499
BciT130I CCWGG 1 cut(s) 927
BclI TGATCA 2 cut(s) 526, 567
BcnI CCSGG 2 cut(s) 125, 1631
BcoDI GTCTC 2 cut(s) 1369, 1596
BfaI CTAG 2 cut(s) 606, 1482
BfmI CTRYAG 1 cut(s) 969
BglII AGATCT 1 cut(s) 1261
BisI GCNGC 2 cut(s) 1101, 1532
BlpI GCTNAGC 1 cut(s) 767
BlsI GCNGC 2 cut(s) 1102, 1533
Bme1390I CCNGG 3 cut(s) 125, 927, 1631
Bme18I GGWCC 1 cut(s) 1428
BmeT110I CYCGRG 2 cut(s) 911, 1368
BmgT120I GGNCC 1 cut(s) 1428
BmiI GGNNCC 2 cut(s) 1429, 1635
BmrFI CCNGG 3 cut(s) 125, 927, 1631
BmrI ACTGGG 2 cut(s) 955, 1669
BmsI GCATC 2 cut(s) 192, 1321
BmuI ACTGGG 2 cut(s) 955, 1669
Bpu10I CCTNAGC 1 cut(s) 465
Bpu1102I GCTNAGC 1 cut(s) 767
Bpu14I TTCGAA 1 cut(s) 40
BpuEI CTTGAG 1 cut(s) 56
BpuMI CCSGG 2 cut(s) 125, 1631
BsaAI YACGTR 1 cut(s) 306
BsaI GGTCTC 1 cut(s) 1596
BsaJI CCNNGG 5 cut(s) 93, 232, 885, 925, 958
BsaWI WCCGGW 5 cut(s) 64, 1568, 1608, 1643, 1650
Bse118I RCCGGY 3 cut(s) 1582, 1601, 1643
Bse1I ACTGG 4 cut(s) 291, 950, 1149, 1675
Bse3DI GCAATG 2 cut(s) 570, 1032
BseBI CCWGG 1 cut(s) 927
BseDI CCNNGG 5 cut(s) 93, 232, 885, 925, 958
BseGI GGATG 3 cut(s) 673, 1333, 1547
BseMI GCAATG 2 cut(s) 570, 1032
BseMII CTCAG 4 cut(s) 479, 696, 758, 1428
BseNI ACTGG 4 cut(s) 291, 950, 1149, 1675
BseRI GAGGAG 2 cut(s) 713, 1454
BseXI GCAGC 2 cut(s) 1087, 1518
Bsh1236I CGCG 3 cut(s) 14, 51, 1588
Bsh1285I CGRYCG 4 cut(s) 124, 1602, 1609, 1644
BshFI GGCC 2 cut(s) 98, 1582
BshTI ACCGGT 1 cut(s) 1643
BsiEI CGRYCG 4 cut(s) 124, 1602, 1609, 1644
BsiHKAI GWGCWC 1 cut(s) 1568
BsiHKCI CYCGRG 2 cut(s) 911, 1368
BslFI GGGAC 1 cut(s) 1441
BsmAI GTCTC 2 cut(s) 1369, 1596
BsmFI GGGAC 1 cut(s) 1441
BsnI GGCC 2 cut(s) 98, 1582
Bso31I GGTCTC 1 cut(s) 1596
BsoBI CYCGRG 2 cut(s) 911, 1368
Bsp119I TTCGAA 1 cut(s) 40
Bsp1286I GDGCHC 1 cut(s) 1568
Bsp143I GATC 4 cut(s) 526, 567, 655, 1261
Bsp1720I GCTNAGC 1 cut(s) 767
Bsp19I CCATGG 2 cut(s) 232, 885
BspACI CCGC 6 cut(s) 12, 128, 704, 1037, 1152, 1588
BspANI GGCC 2 cut(s) 98, 1582
BspCNI CTCAG 4 cut(s) 478, 695, 759, 1429
BspFNI CGCG 3 cut(s) 14, 51, 1588
BspLI GGNNCC 2 cut(s) 1429, 1635
BspPI GGATC 1 cut(s) 650
BspT104I TTCGAA 1 cut(s) 40
BspTNI GGTCTC 1 cut(s) 1596
BsrDI GCAATG 2 cut(s) 570, 1032
BsrFI RCCGGY 3 cut(s) 1582, 1601, 1643
BsrI ACTGG 4 cut(s) 291, 950, 1149, 1675
BssAI RCCGGY 3 cut(s) 1582, 1601, 1643
BssECI CCNNGG 5 cut(s) 93, 232, 885, 925, 958
BssMI GATC 4 cut(s) 526, 567, 655, 1261
BssT1I CCWWGG 4 cut(s) 93, 232, 885, 958
Bst2UI CCWGG 1 cut(s) 927
Bst4CI ACNGT 6 cut(s) 210, 1051, 1064, 1125, 1386, 1625
Bst6I CTCTTC 2 cut(s) 1170, 1290
BstBAI YACGTR 1 cut(s) 306
BstBI TTCGAA 1 cut(s) 40
BstC8I GCNNGC 3 cut(s) 764, 825, 1584
BstDEI CTNAG 8 cut(s) 420, 465, 682, 749, 767, 1068, 1437, 1535
BstDSI CCRYGG 2 cut(s) 232, 885
BstEII GGTNACC 2 cut(s) 1625, 1646
BstENI CCTNNNNNAGG 1 cut(s) 799
BstF5I GGATG 3 cut(s) 673, 1333, 1547
BstFNI CGCG 3 cut(s) 14, 51, 1588
BstHHI GCGC 2 cut(s) 51, 1588
BstKTI GATC 4 cut(s) 529, 570, 658, 1264
BstMAI GTCTC 2 cut(s) 1369, 1596
BstMBI GATC 4 cut(s) 526, 567, 655, 1261
BstMCI CGRYCG 4 cut(s) 124, 1602, 1609, 1644
BstMWI GCNNNNNNNGC 5 cut(s) 759, 768, 1006, 1473, 1667
BstNI CCWGG 1 cut(s) 927
BstPI GGTNACC 2 cut(s) 1625, 1646
BstSCI CCNGG 3 cut(s) 123, 925, 1629
BstSFI CTRYAG 1 cut(s) 969
BstUI CGCG 3 cut(s) 14, 51, 1588
BstV1I GCAGC 2 cut(s) 1087, 1518
BstX2I RGATCY 2 cut(s) 655, 1261
BstXI CCANNNNNNTGG 1 cut(s) 945
BstYI RGATCY 2 cut(s) 655, 1261
BsuRI GGCC 2 cut(s) 98, 1582
BtgI CCRYGG 2 cut(s) 232, 885
BtsCI GGATG 3 cut(s) 673, 1333, 1547
BtsI GCAGTG 1 cut(s) 1483
BtsIMutI CAGTG 3 cut(s) 1047, 1434, 1483
Cac8I GCNNGC 3 cut(s) 764, 825, 1584
CfoI GCGC 2 cut(s) 51, 1588
Cfr10I RCCGGY 3 cut(s) 1582, 1601, 1643
Cfr13I GGNCC 1 cut(s) 1428
CseI GACGC 1 cut(s) 3
Csp6I GTAC 6 cut(s) 160, 284, 307, 496, 831, 1161
CspAI ACCGGT 1 cut(s) 1643
CspCI CAANNNNNGTGG 2 cut(s) 1036, 1071
CviAII CATG 7 cut(s) 34, 88, 233, 550, 565, 886, 1129
CviQI GTAC 6 cut(s) 160, 284, 307, 496, 831, 1161
DdeI CTNAG 8 cut(s) 420, 465, 682, 749, 767, 1068, 1437, 1535
DpnI GATC 4 cut(s) 528, 569, 657, 1263
DpnII GATC 4 cut(s) 526, 567, 655, 1261
DraI TTTAAA 1 cut(s) 138
DrdI GACNNNNNNGTC 1 cut(s) 1025
DseDI GACNNNNNNGTC 1 cut(s) 1025
EaeI YGGCCR 2 cut(s) 96, 1580
Eam1104I CTCTTC 2 cut(s) 1170, 1290
EarI CTCTTC 2 cut(s) 1170, 1290
EciI GGCGGA 2 cut(s) 143, 719
Eco130I CCWWGG 4 cut(s) 93, 232, 885, 958
Eco31I GGTCTC 1 cut(s) 1596
Eco47I GGWCC 1 cut(s) 1428
Eco57I CTGAAG 3 cut(s) 299, 461, 1063
Eco88I CYCGRG 2 cut(s) 911, 1368
Eco91I GGTNACC 2 cut(s) 1625, 1646
EcoNI CCTNNNNNAGG 1 cut(s) 799
EcoO65I GGTNACC 2 cut(s) 1625, 1646
EcoRI GAATTC 1 cut(s) 726
EcoRII CCWGG 1 cut(s) 925
EcoT14I CCWWGG 4 cut(s) 93, 232, 885, 958
ErhI CCWWGG 4 cut(s) 93, 232, 885, 958
FaeI CATG 7 cut(s) 37, 91, 236, 553, 568, 889, 1132
FalI AAGNNNNNCTT 4 cut(s) 434, 466, 1061, 1093
FaqI GGGAC 1 cut(s) 1441
FatI CATG 7 cut(s) 33, 87, 232, 549, 564, 885, 1128
FbaI TGATCA 2 cut(s) 526, 567
Fnu4HI GCNGC 2 cut(s) 1101, 1532
FokI GGATG 3 cut(s) 680, 1340, 1534
Fsp4HI GCNGC 2 cut(s) 1101, 1532
FspBI CTAG 2 cut(s) 606, 1482
GlaI GCGC 2 cut(s) 50, 1587
GluI GCNGC 2 cut(s) 1101, 1532
HaeIII GGCC 2 cut(s) 98, 1582
HgaI GACGC 1 cut(s) 3
HhaI GCGC 2 cut(s) 51, 1588
Hin1II CATG 7 cut(s) 37, 91, 236, 553, 568, 889, 1132
Hin6I GCGC 2 cut(s) 49, 1586
HinP1I GCGC 2 cut(s) 49, 1586
HindIII AAGCTT 2 cut(s) 434, 760
HphI GGTGA 5 cut(s) 331, 1222, 1583, 1637, 1658
Hpy166II GTNNAC 3 cut(s) 162, 562, 817
Hpy188I TCNGA 5 cut(s) 121, 441, 531, 685, 1110
Hpy188III TCNNGA 7 cut(s) 340, 479, 627, 734, 911, 1265, 1370
Hpy8I GTNNAC 3 cut(s) 162, 562, 817
HpyAV CCTTC 4 cut(s) 485, 624, 658, 1149
HpyCH4III ACNGT 6 cut(s) 210, 1051, 1064, 1125, 1386, 1625
HpyCH4IV ACGT 4 cut(s) 305, 577, 717, 1408
HpyCH4V TGCA 9 cut(s) 147, 205, 347, 371, 455, 575, 1103, 1334, 1412
HpyF10VI GCNNNNNNNGC 5 cut(s) 759, 768, 1006, 1473, 1667
HpyF3I CTNAG 8 cut(s) 420, 465, 682, 749, 767, 1068, 1437, 1535
HpySE526I ACGT 4 cut(s) 305, 577, 717, 1408
Hsp92II CATG 7 cut(s) 37, 91, 236, 553, 568, 889, 1132
HspAI GCGC 2 cut(s) 49, 1586
KroI GCCGGC 1 cut(s) 1582
KroNI GCCGGC 1 cut(s) 1584
Ksp22I TGATCA 2 cut(s) 526, 567
Kzo9I GATC 4 cut(s) 526, 567, 655, 1261
LmnI GCTCC 3 cut(s) 250, 1441, 1571
Lsp1109I GCAGC 2 cut(s) 1087, 1518
LweI GCATC 2 cut(s) 192, 1321
MaeI CTAG 2 cut(s) 606, 1482
MaeII ACGT 4 cut(s) 305, 577, 717, 1408
MaeIII GTNAC 4 cut(s) 1058, 1571, 1625, 1646
MalI GATC 4 cut(s) 528, 569, 657, 1263
MboI GATC 4 cut(s) 526, 567, 655, 1261
MflI RGATCY 2 cut(s) 655, 1261
MhlI GDGCHC 1 cut(s) 1568
MlsI TGGCCA 1 cut(s) 98
MluNI TGGCCA 1 cut(s) 98
MlyI GAGTC 2 cut(s) 62, 1606
MmeI TCCRAC 4 cut(s) 144, 499, 1326, 1360
Mox20I TGGCCA 1 cut(s) 98
MroNI GCCGGC 1 cut(s) 1582
MroXI GAANNNNTTC 4 cut(s) 446, 618, 726, 869
MscI TGGCCA 1 cut(s) 98
MseI TTAA 5 cut(s) 137, 756, 932, 1232, 1290
MslI CAYNNNNRTG 2 cut(s) 1176, 1559
Msp20I TGGCCA 1 cut(s) 98
MspR9I CCNGG 3 cut(s) 125, 927, 1631
MvaI CCWGG 1 cut(s) 927
MvnI CGCG 3 cut(s) 14, 51, 1588
MwoI GCNNNNNNNGC 5 cut(s) 759, 768, 1006, 1473, 1667
NaeI GCCGGC 1 cut(s) 1584
NciI CCSGG 2 cut(s) 125, 1631
NcoI CCATGG 2 cut(s) 232, 885
NdeII GATC 4 cut(s) 526, 567, 655, 1261
NgoMIV GCCGGC 1 cut(s) 1582
NlaIII CATG 7 cut(s) 37, 91, 236, 553, 568, 889, 1132
NlaIV GGNNCC 2 cut(s) 1429, 1635
NmuCI GTSAC 3 cut(s) 1571, 1625, 1646
NspV TTCGAA 1 cut(s) 40
OliI CACNNNNGTG 1 cut(s) 1176
PdiI GCCGGC 1 cut(s) 1584
PdmI GAANNNNTTC 4 cut(s) 446, 618, 726, 869
PflFI GACNNNGTC 2 cut(s) 227, 947
PinAI ACCGGT 1 cut(s) 1643
PkrI GCNGC 2 cut(s) 1102, 1533
PleI GAGTC 2 cut(s) 61, 1606
PpsI GAGTC 2 cut(s) 61, 1606
Ppu21I YACGTR 1 cut(s) 306
PshBI ATTAAT 1 cut(s) 1232
Psp6I CCWGG 1 cut(s) 925
PspEI GGTNACC 2 cut(s) 1625, 1646
PspGI CCWGG 1 cut(s) 925
PspN4I GGNNCC 2 cut(s) 1429, 1635
PspPI GGNCC 1 cut(s) 1428
PsuI RGATCY 2 cut(s) 655, 1261
PsyI GACNNNGTC 2 cut(s) 227, 947
RsaI GTAC 6 cut(s) 161, 285, 308, 497, 832, 1162
RsaNI GTAC 6 cut(s) 160, 284, 307, 496, 831, 1161
RseI CAYNNNNRTG 2 cut(s) 1176, 1559
SaqAI TTAA 5 cut(s) 137, 756, 932, 1232, 1290
SatI GCNGC 2 cut(s) 1101, 1532
Sau3AI GATC 4 cut(s) 526, 567, 655, 1261
Sau96I GGNCC 1 cut(s) 1428
SchI GAGTC 2 cut(s) 62, 1606
ScrFI CCNGG 3 cut(s) 125, 927, 1631
SduI GDGCHC 1 cut(s) 1568
SfaNI GCATC 2 cut(s) 192, 1321
SfcI CTRYAG 1 cut(s) 969
SfuI TTCGAA 1 cut(s) 40
SinI GGWCC 1 cut(s) 1428
SmiMI CAYNNNNRTG 2 cut(s) 1176, 1559
SmlI CTYRAG 1 cut(s) 71
SmoI CTYRAG 1 cut(s) 71
SsiI CCGC 6 cut(s) 12, 128, 704, 1037, 1152, 1588
SspI AATATT 3 cut(s) 858, 880, 1525
SspMI CTAG 2 cut(s) 606, 1482
StyD4I CCNGG 3 cut(s) 123, 925, 1629
StyI CCWWGG 4 cut(s) 93, 232, 885, 958
TaaI ACNGT 6 cut(s) 210, 1051, 1064, 1125, 1386, 1625
TaiI ACGT 4 cut(s) 308, 580, 720, 1411
TaqI TCGA 2 cut(s) 40, 192
TatI WGTACW 3 cut(s) 159, 283, 495
Tru1I TTAA 5 cut(s) 137, 756, 932, 1232, 1290
Tru9I TTAA 5 cut(s) 137, 756, 932, 1232, 1290
TscAI CASTG 3 cut(s) 1054, 1441, 1483
TseFI GTSAC 3 cut(s) 1571, 1625, 1646
TseI GCWGC 2 cut(s) 1100, 1531
Tsp45I GTSAC 3 cut(s) 1571, 1625, 1646
TspDTI ATGAA 5 cut(s) 369, 429, 538, 687, 1431
TspGWI ACGGA 2 cut(s) 46, 1366
TspRI CASTG 3 cut(s) 1054, 1441, 1483
Tth111I GACNNNGTC 2 cut(s) 227, 947
VpaK11BI GGWCC 1 cut(s) 1428
VspI ATTAAT 1 cut(s) 1232
XagI CCTNNNNNAGG 1 cut(s) 799
XapI RAATTY 2 cut(s) 295, 726
XcmI CCANNNNNNNNNTGG 1 cut(s) 892
XmnI GAANNNNTTC 4 cut(s) 446, 618, 726, 869
XspI CTAG 2 cut(s) 606, 1482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.