RLG00000022427

Protein of unknown function (DUF3754)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000718
Physical Location & Seq
Forward (+)
83257183 .. 83261742
4560 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022427

Sequence Viewer

Length: 1407 bp
ATGGGCAAGGGCAAGAAAGACATTATTCGATTAGAGCGTGAATCCGTCATTGCAGTTATTAAGCCCAGACTCATCATGACCTTGACCAATCTCATCGAGCAAAGTTCTGATCGGAATGAGTTTTTGAAGCTATGCAAGAGGGTTGAGTACACAATTCGGGCTTGGTATCTTCTACAATTTGAGGATTTGATGCAACTGTACTCCCTCTTTGATCCTGTGCATGGAGCCCAGAGGTTGGAGCAACAGAAACTTGACGATGAAGAAATTGATGTGCTTGAACAAAATTTCCTCATGTACTTGTTTCAGGTGATGGAAAAGAGTAATTTTAAGATTGCAAATAATGAAGAAATTGAGGTTGCAGCTTCAGGGCAGTATCTTCTACGTCTTCCCATCGTGGTTGATGAATCTAAGCTTGACAACAGACTTTTGAAGAAATATTTCGAAGGTCATCCTACCGAAGACATTCCAGATTTTGCTGATAAGTATGTCATCTTCCGACGCGGCATTGGACTTGATAAGACAACCGGTTGGTTTTTCATGGAGAAGGTGGACATTATCATTGCACGTTTTTGGAAATATCTTTTACAAATAACAAAGTTAGACAAGTTATTCAAGAAACCAACCCCTAAGAAGAGGGATTCAGAATCTGATACAGATGAGGACGACTTCTTTGTTGAACGAATCCGTCTTGAAAACATGGAACTAAGCGTTCAAACTTTGCTTCTTAAGAACACAATCCAGGAGCCAACTTTTGATAGGATTATCGTTGTTTACAGACAAGCAACTACCGACGAGGATGGTTCAGAACGGGGAATACATATTAAACATTTCAAAAATATTCCAATGGCTGATTTGGAGATAGTTCTTCCTGAAAAGAAGAACCCAGGTTTAACACCAATGGACTGGCTCTCGTTCATAGGCTCTGCCGTAGTTGGTCTGGTTGCTGTTATTACTTCGATTGAAAATACTAAACAGAACTTAGCTCAATATCAGAACTTGATAACACAATCCATGTATGAAAAACAACTAGATAGTGGAAGGGGAACTCTTCTTCACTTATGTGATGATGTGATTCAACAGGAAGTCAAGGAAGTAATAGTTTCTTTCTATATGTTAATGGAACAAGGGAAAGCTACTTTACCTGATCTGGACAAGTGGTGTGAGGACCTAATCAAAGAACAGTTTAACGATGATATCAATTTTGATGTGGACGATGCAGTTAAGAAGTTAGAGAAATTGGGAATTGTTACTCGGGATACCCTTGGACGGTATAGCGCTGTTGGGCTGAAACGTGCTAACGAGATCATTGGCACCACCACGGAGGAGGTTGTCCTCAAGGCAAAACAAGATGCTGCTAATGCTCCTGTTTCTTCTGCAGCTGCTGCGGTCGCTGCCGCTGGTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3754

Protein Analysis

469

Amino Acids

53.8

Weight (kDa)

5.04

Isoelectric Point (pI)

29.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3754 PF12576 251 - 320 3.6e-12 Protein of unknown function (DUF3754)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1310
AccB7I CCANNNNNTGG 1 cut(s) 235
AccII CGCG 1 cut(s) 501
AciI CCGC 3 cut(s) 501, 1385, 1395
AclWI GGATC 1 cut(s) 206
AcsI RAATTY 1 cut(s) 283
AcuI CTGAAG 1 cut(s) 348
AfaI GTAC 3 cut(s) 149, 200, 296
AfeI AGCGCT 1 cut(s) 1276
AfiI CCNNNNNNNGG 3 cut(s) 221, 235, 1266
AflII CTTAAG 1 cut(s) 725
AgeI ACCGGT 1 cut(s) 524
AjnI CCWGG 2 cut(s) 738, 883
AleI CACNNNNGTG 1 cut(s) 1059
AloI GAACNNNNNNTCC 2 cut(s) 270, 302
AluBI AGCT 6 cut(s) 130, 362, 412, 983, 1133, 1379
AluI AGCT 6 cut(s) 130, 362, 412, 983, 1133, 1379
AlwI GGATC 1 cut(s) 206
AlwNI CAGNNNCTG 2 cut(s) 647, 1382
Ama87I CYCGRG 1 cut(s) 1251
Aor51HI AGCGCT 1 cut(s) 1276
ApeKI GCWGC 6 cut(s) 359, 1352, 1376, 1379, 1382, 1391
ApoI RAATTY 1 cut(s) 283
ArsI GACNNNNNNTTYG 2 cut(s) 653, 685
AsiGI ACCGGT 1 cut(s) 524
Asp700I GAANNNNTTC 2 cut(s) 437, 462
AspLEI GCGC 1 cut(s) 1277
AspS9I GGNCC 1 cut(s) 1165
AsuHPI GGTGA 1 cut(s) 319
AsuII TTCGAA 1 cut(s) 441
AvaI CYCGRG 1 cut(s) 1251
AvaII GGWCC 1 cut(s) 1165
BanI GGYRCC 1 cut(s) 1310
BanII GRGCYC 1 cut(s) 229
BarI GAAGNNNNNNTAC 2 cut(s) 476, 508
BbsI GAAGAC 2 cut(s) 377, 465
BbvI GCAGC 6 cut(s) 371, 1339, 1366, 1369, 1378, 1388
BccI CCATC 3 cut(s) 304, 398, 791
BceAI ACGGC 1 cut(s) 911
BciT130I CCWGG 2 cut(s) 740, 885
BciVI GTATCC 1 cut(s) 1249
BfaI CTAG 1 cut(s) 1028
BfmI CTRYAG 1 cut(s) 1374
BfoI RGCGCY 1 cut(s) 1278
BfrI CTTAAG 1 cut(s) 725
BfuI GTATCC 1 cut(s) 1249
BisI GCNGC 8 cut(s) 360, 502, 1353, 1377, 1380, 1383, 1392, 1395
BlsI GCNGC 8 cut(s) 361, 503, 1354, 1378, 1381, 1384, 1393, 1396
Bme1390I CCNGG 2 cut(s) 740, 885
Bme18I GGWCC 1 cut(s) 1165
BmeT110I CYCGRG 1 cut(s) 1251
BmgT120I GGNCC 1 cut(s) 1165
BmiI GGNNCC 3 cut(s) 226, 744, 1312
BmrFI CCNGG 2 cut(s) 740, 885
BmsI GCATC 3 cut(s) 180, 1204, 1339
BpiI GAAGAC 2 cut(s) 377, 465
Bpu14I TTCGAA 1 cut(s) 441
BpuEI CTTGAG 1 cut(s) 1319
BsaJI CCNNGG 3 cut(s) 883, 1261, 1317
BsaWI WCCGGW 1 cut(s) 524
Bsc4I CCNNNNNNNGG 3 cut(s) 221, 235, 1266
Bse118I RCCGGY 1 cut(s) 524
Bse1I ACTGG 1 cut(s) 908
Bse3DI GCAATG 2 cut(s) 48, 558
BseBI CCWGG 2 cut(s) 740, 885
BseDI CCNNGG 3 cut(s) 883, 1261, 1317
BseGI GGATG 2 cut(s) 448, 802
BseLI CCNNNNNNNGG 3 cut(s) 221, 235, 1266
BseMI GCAATG 2 cut(s) 48, 558
BseNI ACTGG 1 cut(s) 908
BseRI GAGGAG 1 cut(s) 1337
BseXI GCAGC 6 cut(s) 371, 1339, 1366, 1369, 1378, 1388
Bsh1236I CGCG 1 cut(s) 501
Bsh1285I CGRYCG 1 cut(s) 1389
BshNI GGYRCC 1 cut(s) 1310
BshTI ACCGGT 1 cut(s) 524
BsiEI CGRYCG 1 cut(s) 1389
BsiHKCI CYCGRG 1 cut(s) 1251
BsiSI CCGG 1 cut(s) 525
BslI CCNNNNNNNGG 3 cut(s) 221, 235, 1266
BsoBI CYCGRG 1 cut(s) 1251
Bsp119I TTCGAA 1 cut(s) 441
Bsp1286I GDGCHC 1 cut(s) 229
Bsp143I GATC 4 cut(s) 109, 211, 1144, 1302
BspACI CCGC 3 cut(s) 501, 1385, 1395
BspFNI CGCG 1 cut(s) 501
BspHI TCATGA 1 cut(s) 75
BspLI GGNNCC 3 cut(s) 226, 744, 1312
BspMAI CTGCAG 1 cut(s) 1378
BspPI GGATC 1 cut(s) 206
BspT104I TTCGAA 1 cut(s) 441
BspT107I GGYRCC 1 cut(s) 1310
BspTI CTTAAG 1 cut(s) 725
BsrDI GCAATG 2 cut(s) 48, 558
BsrFI RCCGGY 1 cut(s) 524
BsrI ACTGG 1 cut(s) 908
BssAI RCCGGY 1 cut(s) 524
BssECI CCNNGG 3 cut(s) 883, 1261, 1317
BssMI GATC 4 cut(s) 109, 211, 1144, 1302
BssT1I CCWWGG 1 cut(s) 1261
Bst2UI CCWGG 2 cut(s) 740, 885
Bst4CI ACNGT 3 cut(s) 198, 1182, 1269
Bst6I CTCTTC 2 cut(s) 626, 1053
BstAFI CTTAAG 1 cut(s) 725
BstAPI GCANNNNNTGC 1 cut(s) 1382
BstBI TTCGAA 1 cut(s) 441
BstDEI CTNAG 4 cut(s) 408, 627, 704, 979
BstDSI CCRYGG 1 cut(s) 1317
BstF5I GGATG 2 cut(s) 448, 802
BstFNI CGCG 1 cut(s) 501
BstH2I RGCGCY 1 cut(s) 1278
BstHHI GCGC 1 cut(s) 1277
BstKTI GATC 4 cut(s) 112, 214, 1147, 1305
BstMBI GATC 4 cut(s) 109, 211, 1144, 1302
BstMCI CGRYCG 1 cut(s) 1389
BstMWI GCNNNNNNNGC 4 cut(s) 1358, 1382, 1388, 1391
BstNI CCWGG 2 cut(s) 740, 885
BstSCI CCNGG 2 cut(s) 738, 883
BstSFI CTRYAG 1 cut(s) 1374
BstUI CGCG 1 cut(s) 501
BstV1I GCAGC 6 cut(s) 371, 1339, 1366, 1369, 1378, 1388
BstV2I GAAGAC 2 cut(s) 377, 465
BstXI CCANNNNNNTGG 1 cut(s) 903
BsuI GTATCC 1 cut(s) 1249
BtgI CCRYGG 1 cut(s) 1317
BtsCI GGATG 2 cut(s) 448, 802
CaiI CAGNNNCTG 2 cut(s) 647, 1382
CciI TCATGA 1 cut(s) 75
CfoI GCGC 1 cut(s) 1277
Cfr10I RCCGGY 1 cut(s) 524
Cfr13I GGNCC 1 cut(s) 1165
CseI GACGC 1 cut(s) 507
Csp6I GTAC 3 cut(s) 148, 199, 295
CspAI ACCGGT 1 cut(s) 524
CviAII CATG 6 cut(s) 76, 221, 292, 538, 697, 1012
CviQI GTAC 3 cut(s) 148, 199, 295
DdeI CTNAG 4 cut(s) 408, 627, 704, 979
DpnI GATC 4 cut(s) 111, 213, 1146, 1304
DpnII GATC 4 cut(s) 109, 211, 1144, 1302
Eam1104I CTCTTC 2 cut(s) 626, 1053
EarI CTCTTC 2 cut(s) 626, 1053
Eco130I CCWWGG 1 cut(s) 1261
Eco24I GRGCYC 1 cut(s) 229
Eco32I GATATC 1 cut(s) 1195
Eco47I GGWCC 1 cut(s) 1165
Eco47III AGCGCT 1 cut(s) 1276
Eco57I CTGAAG 1 cut(s) 348
Eco88I CYCGRG 1 cut(s) 1251
EcoO109I RGGNCCY 1 cut(s) 1165
EcoRII CCWGG 2 cut(s) 738, 883
EcoRV GATATC 1 cut(s) 1195
EcoT14I CCWWGG 1 cut(s) 1261
EcoT38I GRGCYC 1 cut(s) 229
ErhI CCWWGG 1 cut(s) 1261
FaeI CATG 6 cut(s) 79, 224, 295, 541, 700, 1015
FatI CATG 6 cut(s) 75, 220, 291, 537, 696, 1011
Fnu4HI GCNGC 8 cut(s) 360, 502, 1353, 1377, 1380, 1383, 1392, 1395
FokI GGATG 2 cut(s) 435, 809
FriOI GRGCYC 1 cut(s) 229
Fsp4HI GCNGC 8 cut(s) 360, 502, 1353, 1377, 1380, 1383, 1392, 1395
FspBI CTAG 1 cut(s) 1028
GlaI GCGC 1 cut(s) 1276
GluI GCNGC 8 cut(s) 360, 502, 1353, 1377, 1380, 1383, 1392, 1395
HaeII RGCGCY 1 cut(s) 1278
HapII CCGG 1 cut(s) 525
HgaI GACGC 1 cut(s) 507
HhaI GCGC 1 cut(s) 1277
Hin1II CATG 6 cut(s) 79, 224, 295, 541, 700, 1015
Hin6I GCGC 1 cut(s) 1275
HinP1I GCGC 1 cut(s) 1275
HindIII AAGCTT 1 cut(s) 410
HinfI GANTC 7 cut(s) 41, 69, 404, 638, 644, 681, 1072
HpaII CCGG 1 cut(s) 525
HphI GGTGA 1 cut(s) 319
Hpy166II GTNNAC 4 cut(s) 150, 550, 772, 1210
Hpy188I TCNGA 7 cut(s) 109, 114, 497, 643, 649, 805, 993
Hpy188III TCNNGA 7 cut(s) 76, 467, 613, 689, 869, 1148, 1253
Hpy8I GTNNAC 4 cut(s) 150, 550, 772, 1210
Hpy99I CGWCG 2 cut(s) 501, 794
HpyAV CCTTC 3 cut(s) 437, 538, 1032
HpyCH4III ACNGT 3 cut(s) 198, 1182, 1269
HpyCH4IV ACGT 3 cut(s) 382, 565, 1291
HpyCH4V TGCA 9 cut(s) 53, 135, 193, 220, 335, 359, 563, 1217, 1376
HpyF10VI GCNNNNNNNGC 4 cut(s) 1358, 1382, 1388, 1391
HpyF3I CTNAG 4 cut(s) 408, 627, 704, 979
HpySE526I ACGT 3 cut(s) 382, 565, 1291
Hsp92II CATG 6 cut(s) 79, 224, 295, 541, 700, 1015
HspAI GCGC 1 cut(s) 1275
Kzo9I GATC 4 cut(s) 109, 211, 1144, 1302
LmnI GCTCC 4 cut(s) 224, 238, 742, 1366
Lsp1109I GCAGC 6 cut(s) 371, 1339, 1366, 1369, 1378, 1388
LweI GCATC 3 cut(s) 180, 1204, 1339
MaeI CTAG 1 cut(s) 1028
MaeII ACGT 3 cut(s) 382, 565, 1291
MaeIII GTNAC 1 cut(s) 1246
MalI GATC 4 cut(s) 111, 213, 1146, 1304
MboI GATC 4 cut(s) 109, 211, 1144, 1302
MhlI GDGCHC 1 cut(s) 229
MluCI AATT 9 cut(s) 153, 176, 264, 283, 322, 348, 1198, 1235, 1242
MlyI GAGTC 1 cut(s) 63
MmeI TCCRAC 2 cut(s) 216, 520
MroXI GAANNNNTTC 2 cut(s) 437, 462
MseI TTAA 9 cut(s) 60, 327, 726, 822, 890, 1115, 1185, 1221, 1405
MslI CAYNNNNRTG 1 cut(s) 1059
MspA1I CMGCKG 2 cut(s) 1379, 1397
MspCI CTTAAG 1 cut(s) 725
MspI CCGG 1 cut(s) 525
MspR9I CCNGG 2 cut(s) 740, 885
MvaI CCWGG 2 cut(s) 740, 885
MvnI CGCG 1 cut(s) 501
MwoI GCNNNNNNNGC 4 cut(s) 1358, 1382, 1388, 1391
NdeII GATC 4 cut(s) 109, 211, 1144, 1302
NlaIII CATG 6 cut(s) 79, 224, 295, 541, 700, 1015
NlaIV GGNNCC 3 cut(s) 226, 744, 1312
NspV TTCGAA 1 cut(s) 441
OliI CACNNNNGTG 1 cut(s) 1059
PagI TCATGA 1 cut(s) 75
PcsI WCGNNNNNNNCGW 1 cut(s) 34
PdmI GAANNNNTTC 2 cut(s) 437, 462
PfeI GAWTC 6 cut(s) 41, 404, 638, 644, 681, 1072
PflMI CCANNNNNTGG 1 cut(s) 235
PfoI TCCNGGA 1 cut(s) 738
PinAI ACCGGT 1 cut(s) 524
PkrI GCNGC 8 cut(s) 361, 503, 1354, 1378, 1381, 1384, 1393, 1396
PleI GAGTC 1 cut(s) 63
PpsI GAGTC 1 cut(s) 63
PpuMI RGGWCCY 1 cut(s) 1165
Psp5II RGGWCCY 1 cut(s) 1165
Psp6I CCWGG 2 cut(s) 738, 883
PspGI CCWGG 2 cut(s) 738, 883
PspN4I GGNNCC 3 cut(s) 226, 744, 1312
PspPI GGNCC 1 cut(s) 1165
PspPPI RGGWCCY 1 cut(s) 1165
PsrI GAACNNNNNNTAC 2 cut(s) 798, 830
PstI CTGCAG 1 cut(s) 1378
PstNI CAGNNNCTG 2 cut(s) 647, 1382
PvuII CAGCTG 1 cut(s) 1379
RsaI GTAC 3 cut(s) 149, 200, 296
RsaNI GTAC 3 cut(s) 148, 199, 295
RseI CAYNNNNRTG 1 cut(s) 1059
SaqAI TTAA 9 cut(s) 60, 327, 726, 822, 890, 1115, 1185, 1221, 1405
SatI GCNGC 8 cut(s) 360, 502, 1353, 1377, 1380, 1383, 1392, 1395
Sau3AI GATC 4 cut(s) 109, 211, 1144, 1302
Sau96I GGNCC 1 cut(s) 1165
SchI GAGTC 1 cut(s) 63
ScrFI CCNGG 2 cut(s) 740, 885
SduI GDGCHC 1 cut(s) 229
SfaNI GCATC 3 cut(s) 180, 1204, 1339
SfcI CTRYAG 1 cut(s) 1374
SfuI TTCGAA 1 cut(s) 441
SinI GGWCC 1 cut(s) 1165
SmiMI CAYNNNNRTG 1 cut(s) 1059
SmlI CTYRAG 2 cut(s) 725, 1334
SmoI CTYRAG 2 cut(s) 725, 1334
Sse9I AATT 9 cut(s) 153, 176, 264, 283, 322, 348, 1198, 1235, 1242
SsiI CCGC 3 cut(s) 501, 1385, 1395
SspI AATATT 2 cut(s) 437, 838
SspMI CTAG 1 cut(s) 1028
StyD4I CCNGG 2 cut(s) 738, 883
StyI CCWWGG 1 cut(s) 1261
TaaI ACNGT 3 cut(s) 198, 1182, 1269
TaiI ACGT 3 cut(s) 385, 568, 1294
TaqI TCGA 4 cut(s) 28, 96, 441, 956
TasI AATT 9 cut(s) 153, 176, 264, 283, 322, 348, 1198, 1235, 1242
TatI WGTACW 3 cut(s) 147, 198, 294
TauI GCSGC 2 cut(s) 504, 1397
TfiI GAWTC 6 cut(s) 41, 404, 638, 644, 681, 1072
Tru1I TTAA 9 cut(s) 60, 327, 726, 822, 890, 1115, 1185, 1221, 1405
Tru9I TTAA 9 cut(s) 60, 327, 726, 822, 890, 1115, 1185, 1221, 1405
TseI GCWGC 6 cut(s) 359, 1352, 1376, 1379, 1382, 1391
TspDTI ATGAA 6 cut(s) 273, 357, 417, 526, 904, 1032
TspGWI ACGGA 3 cut(s) 34, 674, 1334
Van91I CCANNNNNTGG 1 cut(s) 235
Vha464I CTTAAG 1 cut(s) 725
VpaK11BI GGWCC 1 cut(s) 1165
XapI RAATTY 1 cut(s) 283
XmnI GAANNNNTTC 2 cut(s) 437, 462
XspI CTAG 1 cut(s) 1028
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.