Rh2AG674200

Protein of unknown function (DUF3754)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000718
Physical Location & Seq
Forward (+)
88630547 .. 88635513
4967 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG674200.1

Sequence Viewer

Length: 1647 bp
ATGGGCAAGAGCAAGAAGAAAGAGATTATACGATTAGAGCGTGAATCCGTCATTGGAGTTACCAAGGCCAGACTCATCAGCACCTTGGCCGCTGAAATCGAAAACGATACTCAGGATCGGACTGAAGAACAGAGAATTCCTGATCGGGATGAGTTTTTGAAGCTATGCAAGAGGGTTGAGTACACAATTCGGGCTTGGTATCTTCTACAATTTGAGGATTTGATGCAACTCTACTCCCTCTTTGATCCTGTACATGGAGCCCAGAAGTTAGAGCAGCAGAAACTTAGTGATAAAGAAATTGATGAGCTTGAACAAAATTTCCTCAAGGACTTATTTCTGGTGATGGAAAAGAGTAATTTTAAGATTGCAAGTAATGAAGAAATTGACATTGCGACTTCAGGGGAGTATCTTCTACATCTTCCCATTGTGGTTGATGAGTCTAAGCTTGACAACAAACTTTTGAAGAAATATTTTGAAAATAATCCTGCCGAAGACATTCCAGATTTTTCTGATAAGTATGTCATCTTCCGACGCGGCATTGGACTAGATAAGAAAACCGATTTCTTTATTATGGAGAAGGTGGACCTGATCATTTCACGTTTTTGGAAATATCTTTTACGAATAACTACGTTAGACAAATTTTTCAAGAAACCAGCCCCTAAGAAGAGGGATTCAGAATCTGATACAGATGAGGATGACTTCTTTGTTGAACGAATCCGTCTTGAAAACATGGAACTAAGCATTCAAAATTTAATTCTACCGAACACAATCCAGGAGCCAACTTTTGATAGGATTATCGTTGTTTACAGGCAAGCAACTACCGACGAGGATAGTCCAGAACGGGCTATACATATTAAACATTTCAAAAATATTCCAATGGCTGATTTGGAGATAGTTCTTCCTGAAAAGAAGAGCCCAGGTTTAACACCAATGGACTGGCTCTCGTTCATTGGCTCTGCTGTAGTTGGTCTGATTGCTGTTTTTACTTCAATTGAAACGACTAAAGTCGATCTTTGGGTTATATTTGCAATTCTTTCCACATTGATTGGTTACTGTGCTAAGACATATTTCAGTTTTCAGCAGAACTTAGCCGATTATCAGAACTTGATAACACAGTCCATGTACGAAAAACAACTAGATAGCGGAAGGGGAACTCTTCTTCACTTGTGTGATGATGTGATTCAACAGGAAGTCAAGGAAGTAATAATTTCTTTCCTTGTGTTAAATGAAAGAGGGAATTCTACTTTACCAGATCTGGACAAGACGTGTGAGTGGATGATGAAAAATTGGTTTAACGACGAGATCAATTTTGATGTGGACGATGCAGTTAAGAAGCTAGAGAAATTGGGAATTGTTACTAAGGATAGCGAGGGACGCTATAGCTCTGTTGGGCTGAAACGTGCTAACGAGATCATTGGCACCACCACTGAGGAGGTTGTCCTTAAGGCAAAAGCAAAAGAAGATGCTGCTGCTGCTGCTGCTGCTAATGCTAATACTCCTGCTTCTCCTGCAGCAACGACTCCTGCAGTCGGCCCTGACAGCGGCGGCAGCCGGAGCGGCAGTGACAACGCCGACGATGATAACTATGCTGCCGACACCGACGAGGATAAATATGCTGCCGACACCGATGTACTCCTAAATAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3754

Protein Analysis

548

Amino Acids

62.42

Weight (kDa)

4.77

Isoelectric Point (pI)

31.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3754 PF12576 262 - 384 4.9e-24 Protein of unknown function (DUF3754)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1418
AccBSI CCGCTC 1 cut(s) 1557
AccII CGCG 1 cut(s) 534
AciI CCGC 6 cut(s) 90, 534, 1143, 1542, 1545, 1557
AclWI GGATC 2 cut(s) 123, 239
AcoI YGGCCR 1 cut(s) 87
AcsI RAATTY 5 cut(s) 135, 316, 638, 748, 1237
AcuI CTGAAG 2 cut(s) 144, 381
AfaI GTAC 4 cut(s) 182, 252, 1124, 1632
AfiI CCNNNNNNNGG 3 cut(s) 254, 1529, 1541
AflII CTTAAG 1 cut(s) 1442
AflIII ACRYGT 1 cut(s) 1265
AjiI CACGTC 1 cut(s) 1266
AjnI CCWGG 2 cut(s) 771, 916
AjuI GAANNNNNNNTTGG 2 cut(s) 36, 68
AleI CACNNNNGTG 1 cut(s) 1167
AloI GAACNNNNNNTCC 2 cut(s) 303, 335
AluBI AGCT 5 cut(s) 163, 307, 445, 1336, 1383
AluI AGCT 5 cut(s) 163, 307, 445, 1336, 1383
AlwI GGATC 2 cut(s) 123, 239
AlwNI CAGNNNCTG 1 cut(s) 680
AoxI GGCC 3 cut(s) 66, 87, 1531
ApoI RAATTY 5 cut(s) 135, 316, 638, 748, 1237
Asp700I GAANNNNTTC 1 cut(s) 495
AspS9I GGNCC 2 cut(s) 583, 1532
AsuHPI GGTGA 1 cut(s) 352
AvaII GGWCC 1 cut(s) 583
BanI GGYRCC 1 cut(s) 1418
BanII GRGCYC 2 cut(s) 262, 917
BarI GAAGNNNNNNTAC 2 cut(s) 509, 541
BbsI GAAGAC 1 cut(s) 498
BccI CCATC 1 cut(s) 337
BciT130I CCWGG 2 cut(s) 773, 918
BclI TGATCA 1 cut(s) 588
BfaI CTAG 3 cut(s) 545, 1136, 1337
BfmI CTRYAG 4 cut(s) 960, 1378, 1509, 1524
BfrI CTTAAG 1 cut(s) 1442
BglI GCCNNNNNGGC 1 cut(s) 1557
BglII AGATCT 1 cut(s) 1252
Bme1390I CCNGG 2 cut(s) 773, 918
Bme18I GGWCC 1 cut(s) 583
BmgBI CACGTC 1 cut(s) 1266
BmgT120I GGNCC 2 cut(s) 583, 1532
BmiI GGNNCC 3 cut(s) 259, 777, 1420
BmrFI CCNGG 2 cut(s) 773, 918
BmsI GCATC 3 cut(s) 213, 1312, 1453
BoxI GACNNNNGTC 1 cut(s) 1004
BpiI GAAGAC 1 cut(s) 498
BpuEI CTTGAG 1 cut(s) 308
BsaJI CCNNGG 3 cut(s) 63, 84, 916
BsaXI ACNNNNNCTCC 2 cut(s) 1546, 1576
Bsc4I CCNNNNNNNGG 3 cut(s) 254, 1529, 1541
Bse1I ACTGG 1 cut(s) 941
Bse3DI GCAATG 1 cut(s) 387
BseBI CCWGG 2 cut(s) 773, 918
BseDI CCNNGG 3 cut(s) 63, 84, 916
BseGI GGATG 3 cut(s) 154, 700, 1281
BseLI CCNNNNNNNGG 3 cut(s) 254, 1529, 1541
BseMI GCAATG 1 cut(s) 387
BseMII CTCAG 2 cut(s) 125, 1419
BseNI ACTGG 1 cut(s) 941
BseRI GAGGAG 1 cut(s) 1445
Bsh1236I CGCG 1 cut(s) 534
BshFI GGCC 3 cut(s) 68, 89, 1533
BshNI GGYRCC 1 cut(s) 1418
BsiSI CCGG 1 cut(s) 1552
BslFI GGGAC 1 cut(s) 1386
BslI CCNNNNNNNGG 3 cut(s) 254, 1529, 1541
BsmFI GGGAC 1 cut(s) 1386
BsmI GAATGC 1 cut(s) 741
BsnI GGCC 3 cut(s) 68, 89, 1533
Bsp1286I GDGCHC 2 cut(s) 262, 917
Bsp1407I TGTACA 1 cut(s) 250
Bsp143I GATC 8 cut(s) 115, 142, 244, 588, 1009, 1252, 1302, 1410
BspACI CCGC 6 cut(s) 90, 534, 1143, 1542, 1545, 1557
BspANI GGCC 3 cut(s) 68, 89, 1533
BspCNI CTCAG 2 cut(s) 124, 1420
BspFNI CGCG 1 cut(s) 534
BspLI GGNNCC 3 cut(s) 259, 777, 1420
BspMAI CTGCAG 2 cut(s) 1513, 1528
BspPI GGATC 2 cut(s) 123, 239
BspQI GCTCTTC 1 cut(s) 905
BspT107I GGYRCC 1 cut(s) 1418
BspTI CTTAAG 1 cut(s) 1442
BsrBI CCGCTC 1 cut(s) 1557
BsrDI GCAATG 1 cut(s) 387
BsrGI TGTACA 1 cut(s) 250
BsrI ACTGG 1 cut(s) 941
BssECI CCNNGG 3 cut(s) 63, 84, 916
BssMI GATC 8 cut(s) 115, 142, 244, 588, 1009, 1252, 1302, 1410
BssT1I CCWWGG 2 cut(s) 63, 84
Bst2UI CCWGG 2 cut(s) 773, 918
Bst4CI ACNGT 2 cut(s) 1055, 1116
Bst6I CTCTTC 3 cut(s) 659, 905, 1161
BstAFI CTTAAG 1 cut(s) 1442
BstAUI TGTACA 1 cut(s) 250
BstC8I GCNNGC 1 cut(s) 813
BstDEI CTNAG 9 cut(s) 111, 284, 441, 660, 737, 1059, 1087, 1359, 1428
BstF5I GGATG 3 cut(s) 154, 700, 1281
BstFNI CGCG 1 cut(s) 534
BstKTI GATC 8 cut(s) 118, 145, 247, 591, 1012, 1255, 1305, 1413
BstMBI GATC 8 cut(s) 115, 142, 244, 588, 1009, 1252, 1302, 1410
BstNI CCWGG 2 cut(s) 773, 918
BstPAI GACNNNNGTC 1 cut(s) 1004
BstSCI CCNGG 2 cut(s) 771, 916
BstSFI CTRYAG 4 cut(s) 960, 1378, 1509, 1524
BstUI CGCG 1 cut(s) 534
BstV2I GAAGAC 1 cut(s) 498
BstX2I RGATCY 1 cut(s) 1252
BstXI CCANNNNNNTGG 1 cut(s) 936
BstYI RGATCY 1 cut(s) 1252
BsuRI GGCC 3 cut(s) 68, 89, 1533
BtrI CACGTC 1 cut(s) 1266
BtsCI GGATG 3 cut(s) 154, 700, 1281
BtsI GCAGTG 1 cut(s) 1567
BtsIMutI CAGTG 2 cut(s) 1425, 1567
Cac8I GCNNGC 1 cut(s) 813
CaiI CAGNNNCTG 1 cut(s) 680
Cfr13I GGNCC 2 cut(s) 583, 1532
CseI GACGC 2 cut(s) 540, 1383
Csp6I GTAC 4 cut(s) 181, 251, 1123, 1631
CspCI CAANNNNNGTGG 2 cut(s) 1027, 1062
CviAII CATG 3 cut(s) 254, 730, 1120
CviQI GTAC 4 cut(s) 181, 251, 1123, 1631
DdeI CTNAG 9 cut(s) 111, 284, 441, 660, 737, 1059, 1087, 1359, 1428
DpnI GATC 8 cut(s) 117, 144, 246, 590, 1011, 1254, 1304, 1412
DpnII GATC 8 cut(s) 115, 142, 244, 588, 1009, 1252, 1302, 1410
EaeI YGGCCR 1 cut(s) 87
Eam1104I CTCTTC 3 cut(s) 659, 905, 1161
EarI CTCTTC 3 cut(s) 659, 905, 1161
Eco130I CCWWGG 2 cut(s) 63, 84
Eco24I GRGCYC 2 cut(s) 262, 917
Eco47I GGWCC 1 cut(s) 583
Eco57I CTGAAG 2 cut(s) 144, 381
EcoRI GAATTC 2 cut(s) 135, 1237
EcoRII CCWGG 2 cut(s) 771, 916
EcoT14I CCWWGG 2 cut(s) 63, 84
EcoT38I GRGCYC 2 cut(s) 262, 917
ErhI CCWWGG 2 cut(s) 63, 84
FaeI CATG 3 cut(s) 257, 733, 1123
FalI AAGNNNNNCTT 2 cut(s) 996, 1028
FaqI GGGAC 1 cut(s) 1386
FatI CATG 3 cut(s) 253, 729, 1119
FbaI TGATCA 1 cut(s) 588
FokI GGATG 3 cut(s) 161, 707, 1288
FriOI GRGCYC 2 cut(s) 262, 917
FspBI CTAG 3 cut(s) 545, 1136, 1337
HaeIII GGCC 3 cut(s) 68, 89, 1533
HapII CCGG 1 cut(s) 1552
HgaI GACGC 2 cut(s) 540, 1383
Hin1II CATG 3 cut(s) 257, 733, 1123
HindIII AAGCTT 1 cut(s) 443
HinfI GANTC 8 cut(s) 44, 72, 437, 671, 677, 714, 1180, 1519
HpaII CCGG 1 cut(s) 1552
HphI GGTGA 1 cut(s) 352
Hpy166II GTNNAC 4 cut(s) 183, 583, 805, 1318
Hpy188I TCNGA 7 cut(s) 120, 511, 530, 676, 682, 972, 1101
Hpy188III TCNNGA 9 cut(s) 113, 140, 146, 500, 646, 722, 836, 902, 1256
Hpy8I GTNNAC 4 cut(s) 183, 583, 805, 1318
Hpy99I CGWCG 5 cut(s) 534, 827, 1301, 1577, 1604
HpyAV CCTTC 2 cut(s) 571, 1140
HpyCH4III ACNGT 2 cut(s) 1055, 1116
HpyCH4IV ACGT 4 cut(s) 598, 629, 1265, 1399
HpyCH4V TGCA 7 cut(s) 168, 226, 368, 1028, 1325, 1511, 1526
HpyF3I CTNAG 9 cut(s) 111, 284, 441, 660, 737, 1059, 1087, 1359, 1428
HpySE526I ACGT 4 cut(s) 598, 629, 1265, 1399
Hsp92II CATG 3 cut(s) 257, 733, 1123
Ksp22I TGATCA 1 cut(s) 588
Kzo9I GATC 8 cut(s) 115, 142, 244, 588, 1009, 1252, 1302, 1410
LguI GCTCTTC 1 cut(s) 905
LmnI GCTCC 3 cut(s) 257, 775, 1554
LweI GCATC 3 cut(s) 213, 1312, 1453
MaeI CTAG 3 cut(s) 545, 1136, 1337
MaeII ACGT 4 cut(s) 598, 629, 1265, 1399
MaeIII GTNAC 4 cut(s) 58, 1049, 1354, 1562
MalI GATC 8 cut(s) 117, 144, 246, 590, 1011, 1254, 1304, 1412
MbiI CCGCTC 1 cut(s) 1557
MboI GATC 8 cut(s) 115, 142, 244, 588, 1009, 1252, 1302, 1410
MfeI CAATTG 1 cut(s) 990
MflI RGATCY 1 cut(s) 1252
MhlI GDGCHC 2 cut(s) 262, 917
MlyI GAGTC 3 cut(s) 66, 446, 1513
MmeI TCCRAC 1 cut(s) 553
MroXI GAANNNNTTC 1 cut(s) 495
MseI TTAA 8 cut(s) 360, 752, 855, 923, 1223, 1293, 1329, 1443
MslI CAYNNNNRTG 1 cut(s) 1167
MspA1I CMGCKG 2 cut(s) 92, 1542
MspCI CTTAAG 1 cut(s) 1442
MspI CCGG 1 cut(s) 1552
MspR9I CCNGG 2 cut(s) 773, 918
MunI CAATTG 1 cut(s) 990
Mva1269I GAATGC 1 cut(s) 741
MvaI CCWGG 2 cut(s) 773, 918
MvnI CGCG 1 cut(s) 534
NdeII GATC 8 cut(s) 115, 142, 244, 588, 1009, 1252, 1302, 1410
NlaIII CATG 3 cut(s) 257, 733, 1123
NlaIV GGNNCC 3 cut(s) 259, 777, 1420
NmuCI GTSAC 1 cut(s) 1562
OliI CACNNNNGTG 1 cut(s) 1167
PciSI GCTCTTC 1 cut(s) 905
PcsI WCGNNNNNNNCGW 1 cut(s) 37
PctI GAATGC 1 cut(s) 741
PdmI GAANNNNTTC 1 cut(s) 495
PfeI GAWTC 5 cut(s) 44, 671, 677, 714, 1180
PfoI TCCNGGA 1 cut(s) 771
PleI GAGTC 3 cut(s) 66, 445, 1513
PpsI GAGTC 3 cut(s) 66, 445, 1513
PshAI GACNNNNGTC 1 cut(s) 1004
Psp6I CCWGG 2 cut(s) 771, 916
PspGI CCWGG 2 cut(s) 771, 916
PspN4I GGNNCC 3 cut(s) 259, 777, 1420
PspPI GGNCC 2 cut(s) 583, 1532
PsrI GAACNNNNNNTAC 2 cut(s) 831, 863
PstI CTGCAG 2 cut(s) 1513, 1528
PstNI CAGNNNCTG 1 cut(s) 680
PsuI RGATCY 1 cut(s) 1252
RsaI GTAC 4 cut(s) 182, 252, 1124, 1632
RsaNI GTAC 4 cut(s) 181, 251, 1123, 1631
RseI CAYNNNNRTG 1 cut(s) 1167
SapI GCTCTTC 1 cut(s) 905
SaqAI TTAA 8 cut(s) 360, 752, 855, 923, 1223, 1293, 1329, 1443
Sau3AI GATC 8 cut(s) 115, 142, 244, 588, 1009, 1252, 1302, 1410
Sau96I GGNCC 2 cut(s) 583, 1532
SchI GAGTC 3 cut(s) 66, 446, 1513
ScrFI CCNGG 2 cut(s) 773, 918
SduI GDGCHC 2 cut(s) 262, 917
SfaNI GCATC 3 cut(s) 213, 1312, 1453
SfcI CTRYAG 4 cut(s) 960, 1378, 1509, 1524
SinI GGWCC 1 cut(s) 583
SmiMI CAYNNNNRTG 1 cut(s) 1167
SmlI CTYRAG 2 cut(s) 323, 1442
SmoI CTYRAG 2 cut(s) 323, 1442
SsiI CCGC 6 cut(s) 90, 534, 1143, 1542, 1545, 1557
SspI AATATT 2 cut(s) 470, 871
SspMI CTAG 3 cut(s) 545, 1136, 1337
StyD4I CCNGG 2 cut(s) 771, 916
StyI CCWWGG 2 cut(s) 63, 84
TaaI ACNGT 2 cut(s) 1055, 1116
TaiI ACGT 4 cut(s) 601, 632, 1268, 1402
TaqI TCGA 2 cut(s) 99, 1008
TatI WGTACW 3 cut(s) 180, 250, 1630
TauI GCSGC 5 cut(s) 92, 537, 1545, 1548, 1560
TfiI GAWTC 5 cut(s) 44, 671, 677, 714, 1180
Tru1I TTAA 8 cut(s) 360, 752, 855, 923, 1223, 1293, 1329, 1443
Tru9I TTAA 8 cut(s) 360, 752, 855, 923, 1223, 1293, 1329, 1443
TscAI CASTG 2 cut(s) 1432, 1567
TseFI GTSAC 1 cut(s) 1562
Tsp45I GTSAC 1 cut(s) 1562
TspDTI ATGAA 4 cut(s) 390, 937, 1242, 1295
TspGWI ACGGA 2 cut(s) 37, 707
TspRI CASTG 2 cut(s) 1432, 1567
Vha464I CTTAAG 1 cut(s) 1442
VpaK11BI GGWCC 1 cut(s) 583
XapI RAATTY 5 cut(s) 135, 316, 638, 748, 1237
XmnI GAANNNNTTC 1 cut(s) 495
XspI CTAG 3 cut(s) 545, 1136, 1337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.