Rw2G055300

Protein of unknown function (DUF3754)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000718
Physical Location & Seq
Forward (+)
82737267 .. 82742134
4868 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G055300.1

Sequence Viewer

Length: 1590 bp
ATGGGCAAGAGCAAGAAGAAAGAGATTATACGATTAGAGCGTGAATCCGTCATTGGAGTTACCAAGGCCAGACTCATCAGCACCTTGGCCGCTGAAATCGAACAGAGAATTCCTGATCGGGATGAGTTTTTGAAGCTATGCAAGAGGGTTGAGTACACAATTCGGGCTTGGTATCTTCTACAATTTGAGGATTTGATGCAACTCTACTCCCTCTTTGATCCTGTACATGGAGCCCAGAAGTTAGAGCAACAGAAACTTAGTGATAAAGAAATTGATGAGCTTGAACAAAATTTCCTCAAGGACTTATTTCTGGTGATGGAAAAGAGTAATTTTAAGATTGCAAGTAATGAAGAAATTGACATTGCGACTTCAGGGGAGTATCTTCTACATCTTCCCATTGTGGTTGATGAGTCTAAGCTTGACAACAAACTTTTGAAGAAATATTTTGAAAATAATCCTGCCGAAGACATTCCAGATTTTTCTGATAAGTATGTCATCTTCCGACGCGGCATTGGACTAGATAAGAAAACCGATTTCTTTATTATGGAGAAGGTGGACCTGATCATTTCGCGTTTTTGGAAATATCTTTTACGAATAACTACGTTAGACAAATTTTTCAAGAAACCAGCCCCTAAGAAGAGGGATTCAGAATCTGATACAGATGAGGATGACTTCTTTGTTGAACGAATCCGTCTTGAAAACATGGAACTAAGCATTCAAAATTTAATTCTACCGAACACAATCCAGGAGCCAACTTTTGATAGGATTATCGTTGTTTACAGGCAAGCAACTACTGATGAGGATAGTCCAGAACGGGCTATACATATTAAACATTTCAAAAATATTCCAATGGCTGATTTGGAGATAGTTCTTCCTGAAAAGAAGAGCCCAGGTTTAACACCAATGGACTGGCTCTCGTTCATTGGCTCTGCTGTAGTTGGTCTGATTGCTGTTTTTACTTCAATTGAAACGACTAAAGTCGATCTTTGGGTTATATTTGCAATTCTTTCCACATTGATTGGTTACTGTGCTAAGACATATTTCAGTTTTCAGCAGAACCTAGCCGATTATCAGAACTTGATAACACAGTCCATGTACGAAAAACAACTAGATAGCGGAAGGGGAACTCTTCTTCACTTGTGTGATGATGTGATTCAACAGGAAGTCAAGGAAGTAATAATTTCTTTCCTTGTGTTAAATGAAAGAGGGAATTCTACTTTACCAGATCTGGACAAGACGTGTGAGTGGATGATGAAAACTTGGTTTAACGACGAGATCAATTTTGATGTGGACGATGCAGTTAAGAAGCTAGAGAAATTGGGAATTGTTACTAAGGATAGCGAGGGACGGTATAGCTCTGTTGGGCTGAAACGTGCTAACGAGATCATTGGCACCACCACTGAGGAGCTTGTCCTTAAGGCAAAAGAAGATGCAGCTGCTAATGCTAATACTCCTGCTTCTCCTGCAGCAACGACTCCTGCAGTCGGCGCTGACAGCGGCGGCAGCGACAACGCCGACGATGATAACTATGCTGCCGACACCGACGAGGATAAATATGCTGCCGACACCGATGTACTCCTAAATAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3754

Protein Analysis

529

Amino Acids

60.44

Weight (kDa)

4.77

Isoelectric Point (pI)

29.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3754 PF12576 253 - 375 4.6e-24 Protein of unknown function (DUF3754)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1391
AccII CGCG 2 cut(s) 507, 571
AciI CCGC 5 cut(s) 90, 507, 1116, 1497, 1500
AclWI GGATC 1 cut(s) 212
AcoI YGGCCR 1 cut(s) 87
AcsI RAATTY 5 cut(s) 108, 289, 611, 721, 1210
AcuI CTGAAG 1 cut(s) 354
AfaI GTAC 4 cut(s) 155, 225, 1097, 1575
AfiI CCNNNNNNNGG 2 cut(s) 227, 1484
AflII CTTAAG 1 cut(s) 1415
AflIII ACRYGT 1 cut(s) 1238
AjiI CACGTC 1 cut(s) 1239
AjnI CCWGG 2 cut(s) 744, 889
AjuI GAANNNNNNNTTGG 2 cut(s) 36, 68
AleI CACNNNNGTG 1 cut(s) 1140
AloI GAACNNNNNNTCC 2 cut(s) 276, 308
AluBI AGCT 7 cut(s) 136, 280, 418, 1309, 1356, 1408, 1436
AluI AGCT 7 cut(s) 136, 280, 418, 1309, 1356, 1408, 1436
AlwI GGATC 1 cut(s) 212
AlwNI CAGNNNCTG 1 cut(s) 653
AoxI GGCC 2 cut(s) 66, 87
ApeKI GCWGC 6 cut(s) 1433, 1436, 1466, 1503, 1532, 1559
ApoI RAATTY 5 cut(s) 108, 289, 611, 721, 1210
Asp700I GAANNNNTTC 1 cut(s) 468
AspLEI GCGC 1 cut(s) 1490
AspS9I GGNCC 1 cut(s) 556
AsuHPI GGTGA 1 cut(s) 325
AvaII GGWCC 1 cut(s) 556
BanI GGYRCC 1 cut(s) 1391
BanII GRGCYC 2 cut(s) 235, 890
BarI GAAGNNNNNNTAC 2 cut(s) 482, 514
BbsI GAAGAC 1 cut(s) 471
BbvI GCAGC 6 cut(s) 1423, 1445, 1478, 1515, 1519, 1546
BccI CCATC 1 cut(s) 310
BciT130I CCWGG 2 cut(s) 746, 891
BclI TGATCA 1 cut(s) 561
BfaI CTAG 4 cut(s) 518, 1061, 1109, 1310
BfmI CTRYAG 3 cut(s) 933, 1464, 1479
BfoI RGCGCY 1 cut(s) 1491
BfrI CTTAAG 1 cut(s) 1415
BglII AGATCT 1 cut(s) 1225
Bme1390I CCNGG 2 cut(s) 746, 891
Bme18I GGWCC 1 cut(s) 556
BmgBI CACGTC 1 cut(s) 1239
BmgT120I GGNCC 1 cut(s) 556
BmiI GGNNCC 3 cut(s) 232, 750, 1393
BmrFI CCNGG 2 cut(s) 746, 891
BmsI GCATC 3 cut(s) 186, 1285, 1420
BoxI GACNNNNGTC 1 cut(s) 977
BpiI GAAGAC 1 cut(s) 471
BpuEI CTTGAG 1 cut(s) 281
BsaJI CCNNGG 3 cut(s) 63, 84, 889
Bsc4I CCNNNNNNNGG 2 cut(s) 227, 1484
Bse1I ACTGG 1 cut(s) 914
Bse3DI GCAATG 1 cut(s) 360
BseBI CCWGG 2 cut(s) 746, 891
BseDI CCNNGG 3 cut(s) 63, 84, 889
BseGI GGATG 3 cut(s) 127, 673, 1254
BseLI CCNNNNNNNGG 2 cut(s) 227, 1484
BseMI GCAATG 1 cut(s) 360
BseMII CTCAG 1 cut(s) 1392
BseNI ACTGG 1 cut(s) 914
BseRI GAGGAG 1 cut(s) 1418
BseXI GCAGC 6 cut(s) 1423, 1445, 1478, 1515, 1519, 1546
Bsh1236I CGCG 2 cut(s) 507, 571
BshFI GGCC 2 cut(s) 68, 89
BshNI GGYRCC 1 cut(s) 1391
BslFI GGGAC 1 cut(s) 1359
BslI CCNNNNNNNGG 2 cut(s) 227, 1484
BsmFI GGGAC 1 cut(s) 1359
BsmI GAATGC 1 cut(s) 714
BsnI GGCC 2 cut(s) 68, 89
Bsp1286I GDGCHC 2 cut(s) 235, 890
Bsp1407I TGTACA 1 cut(s) 223
Bsp143I GATC 7 cut(s) 115, 217, 561, 982, 1225, 1275, 1383
BspACI CCGC 5 cut(s) 90, 507, 1116, 1497, 1500
BspANI GGCC 2 cut(s) 68, 89
BspCNI CTCAG 1 cut(s) 1393
BspFNI CGCG 2 cut(s) 507, 571
BspLI GGNNCC 3 cut(s) 232, 750, 1393
BspMAI CTGCAG 2 cut(s) 1468, 1483
BspPI GGATC 1 cut(s) 212
BspQI GCTCTTC 1 cut(s) 878
BspT107I GGYRCC 1 cut(s) 1391
BspTI CTTAAG 1 cut(s) 1415
BsrDI GCAATG 1 cut(s) 360
BsrGI TGTACA 1 cut(s) 223
BsrI ACTGG 1 cut(s) 914
BssECI CCNNGG 3 cut(s) 63, 84, 889
BssMI GATC 7 cut(s) 115, 217, 561, 982, 1225, 1275, 1383
BssT1I CCWWGG 2 cut(s) 63, 84
Bst2UI CCWGG 2 cut(s) 746, 891
Bst4CI ACNGT 3 cut(s) 1028, 1089, 1350
Bst6I CTCTTC 3 cut(s) 632, 878, 1134
BstAFI CTTAAG 1 cut(s) 1415
BstAUI TGTACA 1 cut(s) 223
BstC8I GCNNGC 1 cut(s) 786
BstDEI CTNAG 7 cut(s) 257, 414, 633, 710, 1032, 1332, 1401
BstF5I GGATG 3 cut(s) 127, 673, 1254
BstFNI CGCG 2 cut(s) 507, 571
BstH2I RGCGCY 1 cut(s) 1491
BstHHI GCGC 1 cut(s) 1490
BstKTI GATC 7 cut(s) 118, 220, 564, 985, 1228, 1278, 1386
BstMBI GATC 7 cut(s) 115, 217, 561, 982, 1225, 1275, 1383
BstMWI GCNNNNNNNGC 5 cut(s) 1442, 1463, 1487, 1494, 1503
BstNI CCWGG 2 cut(s) 746, 891
BstPAI GACNNNNGTC 1 cut(s) 977
BstSCI CCNGG 2 cut(s) 744, 889
BstSFI CTRYAG 3 cut(s) 933, 1464, 1479
BstUI CGCG 2 cut(s) 507, 571
BstV1I GCAGC 6 cut(s) 1423, 1445, 1478, 1515, 1519, 1546
BstV2I GAAGAC 1 cut(s) 471
BstX2I RGATCY 1 cut(s) 1225
BstXI CCANNNNNNTGG 1 cut(s) 909
BstYI RGATCY 1 cut(s) 1225
BsuRI GGCC 2 cut(s) 68, 89
BtrI CACGTC 1 cut(s) 1239
BtsCI GGATG 3 cut(s) 127, 673, 1254
BtsIMutI CAGTG 1 cut(s) 1398
Cac8I GCNNGC 1 cut(s) 786
CaiI CAGNNNCTG 1 cut(s) 653
CfoI GCGC 1 cut(s) 1490
Cfr13I GGNCC 1 cut(s) 556
CseI GACGC 1 cut(s) 513
Csp6I GTAC 4 cut(s) 154, 224, 1096, 1574
CspCI CAANNNNNGTGG 2 cut(s) 1000, 1035
CviAII CATG 3 cut(s) 227, 703, 1093
CviQI GTAC 4 cut(s) 154, 224, 1096, 1574
DdeI CTNAG 7 cut(s) 257, 414, 633, 710, 1032, 1332, 1401
DpnI GATC 7 cut(s) 117, 219, 563, 984, 1227, 1277, 1385
DpnII GATC 7 cut(s) 115, 217, 561, 982, 1225, 1275, 1383
EaeI YGGCCR 1 cut(s) 87
Eam1104I CTCTTC 3 cut(s) 632, 878, 1134
EarI CTCTTC 3 cut(s) 632, 878, 1134
Eco130I CCWWGG 2 cut(s) 63, 84
Eco24I GRGCYC 2 cut(s) 235, 890
Eco47I GGWCC 1 cut(s) 556
Eco57I CTGAAG 1 cut(s) 354
EcoRI GAATTC 2 cut(s) 108, 1210
EcoRII CCWGG 2 cut(s) 744, 889
EcoT14I CCWWGG 2 cut(s) 63, 84
EcoT38I GRGCYC 2 cut(s) 235, 890
ErhI CCWWGG 2 cut(s) 63, 84
FaeI CATG 3 cut(s) 230, 706, 1096
FalI AAGNNNNNCTT 2 cut(s) 969, 1001
FaqI GGGAC 1 cut(s) 1359
FatI CATG 3 cut(s) 226, 702, 1092
FbaI TGATCA 1 cut(s) 561
FokI GGATG 3 cut(s) 134, 680, 1261
FriOI GRGCYC 2 cut(s) 235, 890
FspBI CTAG 4 cut(s) 518, 1061, 1109, 1310
GlaI GCGC 1 cut(s) 1489
HaeII RGCGCY 1 cut(s) 1491
HaeIII GGCC 2 cut(s) 68, 89
HgaI GACGC 1 cut(s) 513
HhaI GCGC 1 cut(s) 1490
Hin1II CATG 3 cut(s) 230, 706, 1096
Hin6I GCGC 1 cut(s) 1488
HinP1I GCGC 1 cut(s) 1488
HindIII AAGCTT 1 cut(s) 416
HinfI GANTC 8 cut(s) 44, 72, 410, 644, 650, 687, 1153, 1474
HphI GGTGA 1 cut(s) 325
Hpy166II GTNNAC 4 cut(s) 156, 556, 778, 1291
Hpy188I TCNGA 6 cut(s) 484, 503, 649, 655, 945, 1074
Hpy188III TCNNGA 8 cut(s) 113, 119, 473, 619, 695, 809, 875, 1229
Hpy8I GTNNAC 4 cut(s) 156, 556, 778, 1291
Hpy99I CGWCG 4 cut(s) 507, 1274, 1520, 1547
HpyAV CCTTC 2 cut(s) 544, 1113
HpyCH4III ACNGT 3 cut(s) 1028, 1089, 1350
HpyCH4IV ACGT 3 cut(s) 602, 1238, 1372
HpyCH4V TGCA 8 cut(s) 141, 199, 341, 1001, 1298, 1433, 1466, 1481
HpyF10VI GCNNNNNNNGC 5 cut(s) 1442, 1463, 1487, 1494, 1503
HpyF3I CTNAG 7 cut(s) 257, 414, 633, 710, 1032, 1332, 1401
HpySE526I ACGT 3 cut(s) 602, 1238, 1372
Hsp92II CATG 3 cut(s) 230, 706, 1096
HspAI GCGC 1 cut(s) 1488
Ksp22I TGATCA 1 cut(s) 561
Kzo9I GATC 7 cut(s) 115, 217, 561, 982, 1225, 1275, 1383
LguI GCTCTTC 1 cut(s) 878
LmnI GCTCC 3 cut(s) 230, 748, 1405
Lsp1109I GCAGC 6 cut(s) 1423, 1445, 1478, 1515, 1519, 1546
LweI GCATC 3 cut(s) 186, 1285, 1420
MaeI CTAG 4 cut(s) 518, 1061, 1109, 1310
MaeII ACGT 3 cut(s) 602, 1238, 1372
MaeIII GTNAC 3 cut(s) 58, 1022, 1327
MalI GATC 7 cut(s) 117, 219, 563, 984, 1227, 1277, 1385
MboI GATC 7 cut(s) 115, 217, 561, 982, 1225, 1275, 1383
MfeI CAATTG 1 cut(s) 963
MflI RGATCY 1 cut(s) 1225
MhlI GDGCHC 2 cut(s) 235, 890
MlyI GAGTC 3 cut(s) 66, 419, 1468
MmeI TCCRAC 1 cut(s) 526
MroXI GAANNNNTTC 1 cut(s) 468
MseI TTAA 8 cut(s) 333, 725, 828, 896, 1196, 1266, 1302, 1416
MslI CAYNNNNRTG 1 cut(s) 1140
MspA1I CMGCKG 3 cut(s) 92, 1436, 1497
MspCI CTTAAG 1 cut(s) 1415
MspR9I CCNGG 2 cut(s) 746, 891
MunI CAATTG 1 cut(s) 963
Mva1269I GAATGC 1 cut(s) 714
MvaI CCWGG 2 cut(s) 746, 891
MvnI CGCG 2 cut(s) 507, 571
MwoI GCNNNNNNNGC 5 cut(s) 1442, 1463, 1487, 1494, 1503
NdeII GATC 7 cut(s) 115, 217, 561, 982, 1225, 1275, 1383
NlaIII CATG 3 cut(s) 230, 706, 1096
NlaIV GGNNCC 3 cut(s) 232, 750, 1393
OliI CACNNNNGTG 1 cut(s) 1140
PciSI GCTCTTC 1 cut(s) 878
PcsI WCGNNNNNNNCGW 1 cut(s) 37
PctI GAATGC 1 cut(s) 714
PdmI GAANNNNTTC 1 cut(s) 468
PfeI GAWTC 5 cut(s) 44, 644, 650, 687, 1153
PfoI TCCNGGA 1 cut(s) 744
PleI GAGTC 3 cut(s) 66, 418, 1468
PpsI GAGTC 3 cut(s) 66, 418, 1468
PshAI GACNNNNGTC 1 cut(s) 977
Psp6I CCWGG 2 cut(s) 744, 889
PspGI CCWGG 2 cut(s) 744, 889
PspN4I GGNNCC 3 cut(s) 232, 750, 1393
PspPI GGNCC 1 cut(s) 556
PsrI GAACNNNNNNTAC 2 cut(s) 804, 836
PstI CTGCAG 2 cut(s) 1468, 1483
PstNI CAGNNNCTG 1 cut(s) 653
PsuI RGATCY 1 cut(s) 1225
PvuII CAGCTG 1 cut(s) 1436
RsaI GTAC 4 cut(s) 155, 225, 1097, 1575
RsaNI GTAC 4 cut(s) 154, 224, 1096, 1574
RseI CAYNNNNRTG 1 cut(s) 1140
SapI GCTCTTC 1 cut(s) 878
SaqAI TTAA 8 cut(s) 333, 725, 828, 896, 1196, 1266, 1302, 1416
Sau3AI GATC 7 cut(s) 115, 217, 561, 982, 1225, 1275, 1383
Sau96I GGNCC 1 cut(s) 556
SchI GAGTC 3 cut(s) 66, 419, 1468
ScrFI CCNGG 2 cut(s) 746, 891
SduI GDGCHC 2 cut(s) 235, 890
SfaNI GCATC 3 cut(s) 186, 1285, 1420
SfcI CTRYAG 3 cut(s) 933, 1464, 1479
SinI GGWCC 1 cut(s) 556
SmiMI CAYNNNNRTG 1 cut(s) 1140
SmlI CTYRAG 2 cut(s) 296, 1415
SmoI CTYRAG 2 cut(s) 296, 1415
SsiI CCGC 5 cut(s) 90, 507, 1116, 1497, 1500
SspI AATATT 2 cut(s) 443, 844
SspMI CTAG 4 cut(s) 518, 1061, 1109, 1310
StyD4I CCNGG 2 cut(s) 744, 889
StyI CCWWGG 2 cut(s) 63, 84
TaaI ACNGT 3 cut(s) 1028, 1089, 1350
TaiI ACGT 3 cut(s) 605, 1241, 1375
TaqI TCGA 2 cut(s) 99, 981
TatI WGTACW 3 cut(s) 153, 223, 1573
TauI GCSGC 4 cut(s) 92, 510, 1500, 1503
TfiI GAWTC 5 cut(s) 44, 644, 650, 687, 1153
Tru1I TTAA 8 cut(s) 333, 725, 828, 896, 1196, 1266, 1302, 1416
Tru9I TTAA 8 cut(s) 333, 725, 828, 896, 1196, 1266, 1302, 1416
TscAI CASTG 1 cut(s) 1405
TseI GCWGC 6 cut(s) 1433, 1436, 1466, 1503, 1532, 1559
TspDTI ATGAA 4 cut(s) 363, 910, 1215, 1268
TspGWI ACGGA 2 cut(s) 37, 680
TspRI CASTG 1 cut(s) 1405
Vha464I CTTAAG 1 cut(s) 1415
VpaK11BI GGWCC 1 cut(s) 556
XapI RAATTY 5 cut(s) 108, 289, 611, 721, 1210
XmnI GAANNNNTTC 1 cut(s) 468
XspI CTAG 4 cut(s) 518, 1061, 1109, 1310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.