Rh2AG674300

Protein of unknown function (DUF3754)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000718
Physical Location & Seq
Forward (+)
88636333 .. 88641124
4792 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG674300.1

Sequence Viewer

Length: 1488 bp
ATGGGCAAGGGCAAGGGCAAGAAAGACATTATTCGATTAGAGCGCGAATCCGTCATTGCAGTTATTAAGCCCAGACTCATCATGACCTTGACCAATCTCATCGAGCAAAGTTCTGATCGGTCTGAGTTTTTGAAGCTATGCAAGAGGGTCGAGTACACAATTCGGGCTTGGTATCTTCTACAATTTGAGGATTTGATGCAACTGTACTCCCTCTTTGATCCTGTACATGGAGCCCAGAGGTTGGAGCAACAGAAACTTGACGATGAAGAAATTGATGTGCTTGAACAAAATTTCCTCATGTACTTATTTCAGGTGATGGAAAAGAGTAATTTTAAGATTGCAAATAATGAAGAAATTGAGGTTGCGGCTTCAGGGCAGTATCTTCTACGTCTTCCCATCGTGGTTGATGAGTCTAAGCTTGACAACAGACTTTTGAAGAAATATTTCGAAGGTCATCCTACCGAAGACATTCCAGATTTTGCTGATAAGTATGTCATCTTCCGACGCGGCATTGGACTTGATAAGACAACCGGTTGGTTTTTCATGGAGAAGGTGGACATTATCATTGCACGTTTTTGGAAATATCTTTTACAAATAACAAAGTTAGACAAGTTTTTCAAGAAACCAACCCCTAAGAAGAGGGATTCAGAATCTGATACAGATGAGGACGACTTCTTTGTTGAACGAATCCGTCTTGAAAACATGGAACTAAGTGTTCAAACTTTGCTTCTTAAGAACACAATCCAGGAGCCGACTTTTGATAGGATTATCGTTGTTTACAGACAAGCAACTACCGACGAGGATGGTTCAGAACGGGGAATACATATTAAACATTTCAAAAATATTCCAATGGCTGATTTGGAGATAGTTCTTCCTGAAAAGAAGAACCCAGGTTTAACACCAATGGACTGGCTCTCGTTCATAGGCTCTGCTGTAGTTGGTCTGGTTGCTGTTATTACTTCGATTGAAACGACTAAAGTCGATCTTTGGGTTATGTTTGCAATTCTTTCCACTGTGATTGGTTACTGTGCTAAGACATATTTCAGTTTTCAGCAGAACTTAGCTCAATATCAGAACTTGATAACACAATCCATGTACGAAAAACAACTAGATAGTGGAAGGGGAACTCTTCTTCACTTGTGTGATGATGTGATTCAACAGGAAGTCAAGGAAGTAATAGTTTCTTTCTATATGTTAATGGAACAAGGGAAAGCTACTTTACCTGATCTGGACAAGTGGTGTGAGGACCTAATCAAAGAACAGTTTAACGACGATATCAATTTTGATGTGGACGATGCAGTTAAGAAGTTAGAGAAATTGGGAATTGTTACTCGGGATACCCTTGGACGGTATAGCGCTGTTGGGCTGAAACGTGCTAACGAGATCATTGGCACCACCACTGAGGAGGTTGTCCTCAAGGCAAAACAAGATGCTGCTAATGCTCCTGCTTCTTCTGCAGCTGCTGCGGTTGCCGCCGCTGGTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3754

Protein Analysis

495

Amino Acids

56.83

Weight (kDa)

5.09

Isoelectric Point (pI)

29.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3754 PF12576 253 - 375 3.2e-24 Protein of unknown function (DUF3754)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1391
AccB7I CCANNNNNTGG 1 cut(s) 241
AccII CGCG 2 cut(s) 45, 507
AciI CCGC 5 cut(s) 365, 507, 1466, 1473, 1476
AclWI GGATC 1 cut(s) 212
AcsI RAATTY 1 cut(s) 289
AcuI CTGAAG 1 cut(s) 354
AfaI GTAC 5 cut(s) 155, 206, 225, 302, 1097
AfeI AGCGCT 1 cut(s) 1357
AfiI CCNNNNNNNGG 3 cut(s) 227, 241, 1347
AflII CTTAAG 1 cut(s) 731
AgeI ACCGGT 1 cut(s) 530
AjnI CCWGG 2 cut(s) 744, 889
AleI CACNNNNGTG 1 cut(s) 1140
AloI GAACNNNNNNTCC 2 cut(s) 276, 308
AluBI AGCT 5 cut(s) 136, 418, 1064, 1214, 1460
AluI AGCT 5 cut(s) 136, 418, 1064, 1214, 1460
AlwI GGATC 1 cut(s) 212
AlwNI CAGNNNCTG 2 cut(s) 653, 1463
Ama87I CYCGRG 1 cut(s) 1332
Aor51HI AGCGCT 1 cut(s) 1357
ApeKI GCWGC 4 cut(s) 1433, 1457, 1460, 1463
ApoI RAATTY 1 cut(s) 289
ArsI GACNNNNNNTTYG 2 cut(s) 659, 691
AsiGI ACCGGT 1 cut(s) 530
Asp700I GAANNNNTTC 2 cut(s) 443, 468
AspLEI GCGC 2 cut(s) 45, 1358
AspS9I GGNCC 1 cut(s) 1246
AsuHPI GGTGA 1 cut(s) 325
AsuII TTCGAA 1 cut(s) 447
AvaI CYCGRG 1 cut(s) 1332
AvaII GGWCC 1 cut(s) 1246
BanI GGYRCC 1 cut(s) 1391
BanII GRGCYC 1 cut(s) 235
BarI GAAGNNNNNNTAC 2 cut(s) 482, 514
BbsI GAAGAC 2 cut(s) 383, 471
BbvI GCAGC 4 cut(s) 1420, 1447, 1450, 1469
BccI CCATC 3 cut(s) 310, 404, 797
BcgI CGANNNNNNTGC 2 cut(s) 130, 164
BciT130I CCWGG 2 cut(s) 746, 891
BciVI GTATCC 1 cut(s) 1330
BfaI CTAG 1 cut(s) 1109
BfmI CTRYAG 2 cut(s) 933, 1455
BfoI RGCGCY 1 cut(s) 1359
BfrI CTTAAG 1 cut(s) 731
BfuI GTATCC 1 cut(s) 1330
BisI GCNGC 8 cut(s) 366, 508, 1434, 1458, 1461, 1464, 1473, 1476
BlsI GCNGC 8 cut(s) 367, 509, 1435, 1459, 1462, 1465, 1474, 1477
Bme1390I CCNGG 2 cut(s) 746, 891
Bme18I GGWCC 1 cut(s) 1246
BmeT110I CYCGRG 1 cut(s) 1332
BmgT120I GGNCC 1 cut(s) 1246
BmiI GGNNCC 3 cut(s) 232, 750, 1393
BmrFI CCNGG 2 cut(s) 746, 891
BmsI GCATC 3 cut(s) 186, 1285, 1420
BoxI GACNNNNGTC 1 cut(s) 977
BpiI GAAGAC 2 cut(s) 383, 471
Bpu14I TTCGAA 1 cut(s) 447
BpuEI CTTGAG 1 cut(s) 1400
BsaJI CCNNGG 2 cut(s) 889, 1342
BsaWI WCCGGW 1 cut(s) 530
Bsc4I CCNNNNNNNGG 3 cut(s) 227, 241, 1347
Bse118I RCCGGY 1 cut(s) 530
Bse1I ACTGG 1 cut(s) 914
Bse3DI GCAATG 2 cut(s) 54, 564
BseBI CCWGG 2 cut(s) 746, 891
BseDI CCNNGG 2 cut(s) 889, 1342
BseGI GGATG 2 cut(s) 454, 808
BseLI CCNNNNNNNGG 3 cut(s) 227, 241, 1347
BseMI GCAATG 2 cut(s) 54, 564
BseMII CTCAG 2 cut(s) 114, 1392
BseNI ACTGG 1 cut(s) 914
BseRI GAGGAG 1 cut(s) 1418
BseXI GCAGC 4 cut(s) 1420, 1447, 1450, 1469
Bsh1236I CGCG 2 cut(s) 45, 507
BshNI GGYRCC 1 cut(s) 1391
BshTI ACCGGT 1 cut(s) 530
BsiHKCI CYCGRG 1 cut(s) 1332
BsiSI CCGG 1 cut(s) 531
BslI CCNNNNNNNGG 3 cut(s) 227, 241, 1347
BsoBI CYCGRG 1 cut(s) 1332
Bsp119I TTCGAA 1 cut(s) 447
Bsp1286I GDGCHC 1 cut(s) 235
Bsp1407I TGTACA 1 cut(s) 223
Bsp143I GATC 5 cut(s) 115, 217, 982, 1225, 1383
BspACI CCGC 5 cut(s) 365, 507, 1466, 1473, 1476
BspCNI CTCAG 2 cut(s) 115, 1393
BspFNI CGCG 2 cut(s) 45, 507
BspHI TCATGA 1 cut(s) 81
BspLI GGNNCC 3 cut(s) 232, 750, 1393
BspMAI CTGCAG 1 cut(s) 1459
BspPI GGATC 1 cut(s) 212
BspT104I TTCGAA 1 cut(s) 447
BspT107I GGYRCC 1 cut(s) 1391
BspTI CTTAAG 1 cut(s) 731
BsrDI GCAATG 2 cut(s) 54, 564
BsrFI RCCGGY 1 cut(s) 530
BsrGI TGTACA 1 cut(s) 223
BsrI ACTGG 1 cut(s) 914
BssAI RCCGGY 1 cut(s) 530
BssECI CCNNGG 2 cut(s) 889, 1342
BssMI GATC 5 cut(s) 115, 217, 982, 1225, 1383
BssT1I CCWWGG 1 cut(s) 1342
Bst2UI CCWGG 2 cut(s) 746, 891
Bst4CI ACNGT 5 cut(s) 204, 1015, 1028, 1263, 1350
Bst6I CTCTTC 2 cut(s) 632, 1134
BstAFI CTTAAG 1 cut(s) 731
BstAPI GCANNNNNTGC 1 cut(s) 1463
BstAUI TGTACA 1 cut(s) 223
BstBI TTCGAA 1 cut(s) 447
BstDEI CTNAG 7 cut(s) 123, 414, 633, 710, 1032, 1060, 1401
BstF5I GGATG 2 cut(s) 454, 808
BstFNI CGCG 2 cut(s) 45, 507
BstH2I RGCGCY 1 cut(s) 1359
BstHHI GCGC 2 cut(s) 45, 1358
BstKTI GATC 5 cut(s) 118, 220, 985, 1228, 1386
BstMBI GATC 5 cut(s) 115, 217, 982, 1225, 1383
BstMWI GCNNNNNNNGC 5 cut(s) 1439, 1454, 1463, 1469, 1472
BstNI CCWGG 2 cut(s) 746, 891
BstPAI GACNNNNGTC 1 cut(s) 977
BstSCI CCNGG 2 cut(s) 744, 889
BstSFI CTRYAG 2 cut(s) 933, 1455
BstUI CGCG 2 cut(s) 45, 507
BstV1I GCAGC 4 cut(s) 1420, 1447, 1450, 1469
BstV2I GAAGAC 2 cut(s) 383, 471
BstXI CCANNNNNNTGG 1 cut(s) 909
BsuI GTATCC 1 cut(s) 1330
BtsCI GGATG 2 cut(s) 454, 808
BtsIMutI CAGTG 2 cut(s) 1011, 1398
CaiI CAGNNNCTG 2 cut(s) 653, 1463
CciI TCATGA 1 cut(s) 81
CfoI GCGC 2 cut(s) 45, 1358
Cfr10I RCCGGY 1 cut(s) 530
Cfr13I GGNCC 1 cut(s) 1246
CseI GACGC 1 cut(s) 513
Csp6I GTAC 5 cut(s) 154, 205, 224, 301, 1096
CspAI ACCGGT 1 cut(s) 530
CspCI CAANNNNNGTGG 2 cut(s) 1000, 1035
CviAII CATG 6 cut(s) 82, 227, 298, 544, 703, 1093
CviQI GTAC 5 cut(s) 154, 205, 224, 301, 1096
DdeI CTNAG 7 cut(s) 123, 414, 633, 710, 1032, 1060, 1401
DpnI GATC 5 cut(s) 117, 219, 984, 1227, 1385
DpnII GATC 5 cut(s) 115, 217, 982, 1225, 1383
Eam1104I CTCTTC 2 cut(s) 632, 1134
EarI CTCTTC 2 cut(s) 632, 1134
Eco130I CCWWGG 1 cut(s) 1342
Eco24I GRGCYC 1 cut(s) 235
Eco32I GATATC 1 cut(s) 1276
Eco47I GGWCC 1 cut(s) 1246
Eco47III AGCGCT 1 cut(s) 1357
Eco57I CTGAAG 1 cut(s) 354
Eco88I CYCGRG 1 cut(s) 1332
EcoO109I RGGNCCY 1 cut(s) 1246
EcoRII CCWGG 2 cut(s) 744, 889
EcoRV GATATC 1 cut(s) 1276
EcoT14I CCWWGG 1 cut(s) 1342
EcoT38I GRGCYC 1 cut(s) 235
ErhI CCWWGG 1 cut(s) 1342
FaeI CATG 6 cut(s) 85, 230, 301, 547, 706, 1096
FalI AAGNNNNNCTT 2 cut(s) 969, 1001
FatI CATG 6 cut(s) 81, 226, 297, 543, 702, 1092
Fnu4HI GCNGC 8 cut(s) 366, 508, 1434, 1458, 1461, 1464, 1473, 1476
FokI GGATG 2 cut(s) 441, 815
FriOI GRGCYC 1 cut(s) 235
Fsp4HI GCNGC 8 cut(s) 366, 508, 1434, 1458, 1461, 1464, 1473, 1476
FspBI CTAG 1 cut(s) 1109
GlaI GCGC 2 cut(s) 44, 1357
GluI GCNGC 8 cut(s) 366, 508, 1434, 1458, 1461, 1464, 1473, 1476
HaeII RGCGCY 1 cut(s) 1359
HapII CCGG 1 cut(s) 531
HgaI GACGC 1 cut(s) 513
HhaI GCGC 2 cut(s) 45, 1358
Hin1II CATG 6 cut(s) 85, 230, 301, 547, 706, 1096
Hin6I GCGC 2 cut(s) 43, 1356
HinP1I GCGC 2 cut(s) 43, 1356
HindIII AAGCTT 1 cut(s) 416
HinfI GANTC 7 cut(s) 47, 75, 410, 644, 650, 687, 1153
HpaII CCGG 1 cut(s) 531
HphI GGTGA 1 cut(s) 325
Hpy166II GTNNAC 4 cut(s) 156, 556, 778, 1291
Hpy188I TCNGA 7 cut(s) 115, 124, 503, 649, 655, 811, 1074
Hpy188III TCNNGA 7 cut(s) 82, 473, 619, 695, 875, 1229, 1334
Hpy8I GTNNAC 4 cut(s) 156, 556, 778, 1291
Hpy99I CGWCG 3 cut(s) 507, 800, 1274
HpyAV CCTTC 3 cut(s) 443, 544, 1113
HpyCH4III ACNGT 5 cut(s) 204, 1015, 1028, 1263, 1350
HpyCH4IV ACGT 3 cut(s) 388, 571, 1372
HpyCH4V TGCA 8 cut(s) 59, 141, 199, 341, 569, 1001, 1298, 1457
HpyF10VI GCNNNNNNNGC 5 cut(s) 1439, 1454, 1463, 1469, 1472
HpyF3I CTNAG 7 cut(s) 123, 414, 633, 710, 1032, 1060, 1401
HpySE526I ACGT 3 cut(s) 388, 571, 1372
Hsp92II CATG 6 cut(s) 85, 230, 301, 547, 706, 1096
HspAI GCGC 2 cut(s) 43, 1356
Kzo9I GATC 5 cut(s) 115, 217, 982, 1225, 1383
LmnI GCTCC 4 cut(s) 230, 244, 748, 1447
Lsp1109I GCAGC 4 cut(s) 1420, 1447, 1450, 1469
LweI GCATC 3 cut(s) 186, 1285, 1420
MaeI CTAG 1 cut(s) 1109
MaeII ACGT 3 cut(s) 388, 571, 1372
MaeIII GTNAC 2 cut(s) 1022, 1327
MalI GATC 5 cut(s) 117, 219, 984, 1227, 1385
MboI GATC 5 cut(s) 115, 217, 982, 1225, 1383
MhlI GDGCHC 1 cut(s) 235
MlyI GAGTC 2 cut(s) 69, 419
MmeI TCCRAC 2 cut(s) 222, 526
MroXI GAANNNNTTC 2 cut(s) 443, 468
MseI TTAA 9 cut(s) 66, 333, 732, 828, 896, 1196, 1266, 1302, 1486
MslI CAYNNNNRTG 1 cut(s) 1140
MspA1I CMGCKG 2 cut(s) 1460, 1478
MspCI CTTAAG 1 cut(s) 731
MspI CCGG 1 cut(s) 531
MspR9I CCNGG 2 cut(s) 746, 891
MvaI CCWGG 2 cut(s) 746, 891
MvnI CGCG 2 cut(s) 45, 507
MwoI GCNNNNNNNGC 5 cut(s) 1439, 1454, 1463, 1469, 1472
NdeII GATC 5 cut(s) 115, 217, 982, 1225, 1383
NlaIII CATG 6 cut(s) 85, 230, 301, 547, 706, 1096
NlaIV GGNNCC 3 cut(s) 232, 750, 1393
NspV TTCGAA 1 cut(s) 447
OliI CACNNNNGTG 1 cut(s) 1140
PagI TCATGA 1 cut(s) 81
PcsI WCGNNNNNNNCGW 1 cut(s) 968
PdmI GAANNNNTTC 2 cut(s) 443, 468
PfeI GAWTC 5 cut(s) 47, 644, 650, 687, 1153
PflMI CCANNNNNTGG 1 cut(s) 241
PfoI TCCNGGA 1 cut(s) 744
PinAI ACCGGT 1 cut(s) 530
PkrI GCNGC 8 cut(s) 367, 509, 1435, 1459, 1462, 1465, 1474, 1477
PleI GAGTC 2 cut(s) 69, 418
PpsI GAGTC 2 cut(s) 69, 418
PpuMI RGGWCCY 1 cut(s) 1246
PshAI GACNNNNGTC 1 cut(s) 977
Psp5II RGGWCCY 1 cut(s) 1246
Psp6I CCWGG 2 cut(s) 744, 889
PspGI CCWGG 2 cut(s) 744, 889
PspN4I GGNNCC 3 cut(s) 232, 750, 1393
PspPI GGNCC 1 cut(s) 1246
PspPPI RGGWCCY 1 cut(s) 1246
PsrI GAACNNNNNNTAC 2 cut(s) 804, 836
PstI CTGCAG 1 cut(s) 1459
PstNI CAGNNNCTG 2 cut(s) 653, 1463
PvuII CAGCTG 1 cut(s) 1460
RsaI GTAC 5 cut(s) 155, 206, 225, 302, 1097
RsaNI GTAC 5 cut(s) 154, 205, 224, 301, 1096
RseI CAYNNNNRTG 1 cut(s) 1140
SaqAI TTAA 9 cut(s) 66, 333, 732, 828, 896, 1196, 1266, 1302, 1486
SatI GCNGC 8 cut(s) 366, 508, 1434, 1458, 1461, 1464, 1473, 1476
Sau3AI GATC 5 cut(s) 115, 217, 982, 1225, 1383
Sau96I GGNCC 1 cut(s) 1246
SchI GAGTC 2 cut(s) 69, 419
ScrFI CCNGG 2 cut(s) 746, 891
SduI GDGCHC 1 cut(s) 235
SfaNI GCATC 3 cut(s) 186, 1285, 1420
SfcI CTRYAG 2 cut(s) 933, 1455
SfuI TTCGAA 1 cut(s) 447
SinI GGWCC 1 cut(s) 1246
SmiMI CAYNNNNRTG 1 cut(s) 1140
SmlI CTYRAG 2 cut(s) 731, 1415
SmoI CTYRAG 2 cut(s) 731, 1415
SsiI CCGC 5 cut(s) 365, 507, 1466, 1473, 1476
SspI AATATT 2 cut(s) 443, 844
SspMI CTAG 1 cut(s) 1109
StyD4I CCNGG 2 cut(s) 744, 889
StyI CCWWGG 1 cut(s) 1342
TaaI ACNGT 5 cut(s) 204, 1015, 1028, 1263, 1350
TaiI ACGT 3 cut(s) 391, 574, 1375
TaqI TCGA 6 cut(s) 34, 102, 150, 447, 962, 981
TaqII GACCGA 1 cut(s) 108
TatI WGTACW 4 cut(s) 153, 204, 223, 300
TauI GCSGC 4 cut(s) 368, 510, 1475, 1478
TfiI GAWTC 5 cut(s) 47, 644, 650, 687, 1153
Tru1I TTAA 9 cut(s) 66, 333, 732, 828, 896, 1196, 1266, 1302, 1486
Tru9I TTAA 9 cut(s) 66, 333, 732, 828, 896, 1196, 1266, 1302, 1486
TscAI CASTG 2 cut(s) 1018, 1405
TseI GCWGC 4 cut(s) 1433, 1457, 1460, 1463
TspDTI ATGAA 4 cut(s) 279, 363, 532, 910
TspGWI ACGGA 2 cut(s) 40, 680
TspRI CASTG 2 cut(s) 1018, 1405
Van91I CCANNNNNTGG 1 cut(s) 241
Vha464I CTTAAG 1 cut(s) 731
VpaK11BI GGWCC 1 cut(s) 1246
XapI RAATTY 1 cut(s) 289
XmnI GAANNNNTTC 2 cut(s) 443, 468
XspI CTAG 1 cut(s) 1109
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.