Protein of unknown function (DUF3754)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 307 |
| AfaI | GTAC | 3 cut(s) | 182, 252, 320 |
| AgsI | TTSAA | 2 cut(s) | 93, 352 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 35 |
| AjnI | CCWGG | 1 cut(s) | 144 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 294, 326 |
| AluBI | AGCT | 6 cut(s) | 46, 60, 85, 163, 436, 447 |
| AluI | AGCT | 6 cut(s) | 46, 60, 85, 163, 436, 447 |
| Alw21I | GWGCWC | 1 cut(s) | 48 |
| ApeKI | GCWGC | 1 cut(s) | 444 |
| ApoI | RAATTY | 1 cut(s) | 307 |
| AsuHPI | GGTGA | 2 cut(s) | 62, 343 |
| BanII | GRGCYC | 1 cut(s) | 48 |
| BbsI | GAAGAC | 1 cut(s) | 401 |
| Bbv12I | GWGCWC | 1 cut(s) | 48 |
| BccI | CCATC | 3 cut(s) | 7, 328, 434 |
| BceAI | ACGGC | 1 cut(s) | 20 |
| BciT130I | CCWGG | 1 cut(s) | 146 |
| BisI | GCNGC | 1 cut(s) | 445 |
| BlsI | GCNGC | 1 cut(s) | 446 |
| Bme1390I | CCNGG | 1 cut(s) | 146 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 35 |
| BmrFI | CCNGG | 1 cut(s) | 146 |
| BmsI | GCATC | 1 cut(s) | 213 |
| BpiI | GAAGAC | 1 cut(s) | 401 |
| BsaWI | WCCGGW | 1 cut(s) | 54 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 105, 135 |
| Bse1I | ACTGG | 1 cut(s) | 303 |
| BseBI | CCWGG | 1 cut(s) | 146 |
| BseNI | ACTGG | 1 cut(s) | 303 |
| BsiHKAI | GWGCWC | 1 cut(s) | 48 |
| BsiSI | CCGG | 1 cut(s) | 55 |
| Bsp1286I | GDGCHC | 1 cut(s) | 48 |
| Bsp143I | GATC | 1 cut(s) | 95 |
| BsrI | ACTGG | 1 cut(s) | 303 |
| BssMI | GATC | 1 cut(s) | 95 |
| Bst2UI | CCWGG | 1 cut(s) | 146 |
| Bst4CI | ACNGT | 1 cut(s) | 231 |
| BstC8I | GCNNGC | 1 cut(s) | 44 |
| BstDEI | CTNAG | 2 cut(s) | 404, 432 |
| BstEII | GGTNACC | 1 cut(s) | 50 |
| BstKTI | GATC | 1 cut(s) | 98 |
| BstMBI | GATC | 1 cut(s) | 95 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 391 |
| BstNI | CCWGG | 1 cut(s) | 146 |
| BstPI | GGTNACC | 1 cut(s) | 50 |
| BstSCI | CCNGG | 1 cut(s) | 144 |
| BstV2I | GAAGAC | 1 cut(s) | 401 |
| BtsI | GCAGTG | 1 cut(s) | 98 |
| BtsIMutI | CAGTG | 1 cut(s) | 98 |
| Cac8I | GCNNGC | 1 cut(s) | 44 |
| Csp6I | GTAC | 3 cut(s) | 181, 251, 319 |
| CviAII | CATG | 1 cut(s) | 316 |
| CviJI | RGCY | 8 cut(s) | 21, 46, 60, 85, 163, 194, 436, 447 |
| CviKI_1 | RGCY | 8 cut(s) | 21, 46, 60, 85, 163, 194, 436, 447 |
| CviQI | GTAC | 3 cut(s) | 181, 251, 319 |
| DdeI | CTNAG | 2 cut(s) | 404, 432 |
| DpnI | GATC | 1 cut(s) | 97 |
| DpnII | GATC | 1 cut(s) | 95 |
| DraI | TTTAAA | 1 cut(s) | 159 |
| DriI | GACNNNNNGTC | 1 cut(s) | 35 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 35 |
| Ecl136II | GAGCTC | 1 cut(s) | 46 |
| Eco24I | GRGCYC | 1 cut(s) | 48 |
| Eco53kI | GAGCTC | 1 cut(s) | 46 |
| Eco91I | GGTNACC | 1 cut(s) | 50 |
| EcoICRI | GAGCTC | 1 cut(s) | 46 |
| EcoO65I | GGTNACC | 1 cut(s) | 50 |
| EcoRII | CCWGG | 1 cut(s) | 144 |
| EcoT38I | GRGCYC | 1 cut(s) | 48 |
| FaeI | CATG | 1 cut(s) | 319 |
| FaiI | YATR | 3 cut(s) | 135, 296, 317 |
| FatI | CATG | 1 cut(s) | 315 |
| Fnu4HI | GCNGC | 1 cut(s) | 445 |
| FriOI | GRGCYC | 1 cut(s) | 48 |
| Fsp4HI | GCNGC | 1 cut(s) | 445 |
| GluI | GCNGC | 1 cut(s) | 445 |
| HapII | CCGG | 1 cut(s) | 55 |
| Hin1II | CATG | 1 cut(s) | 319 |
| HindIII | AAGCTT | 1 cut(s) | 434 |
| HinfI | GANTC | 4 cut(s) | 187, 271, 379, 428 |
| HpaII | CCGG | 1 cut(s) | 55 |
| HphI | GGTGA | 2 cut(s) | 62, 343 |
| Hpy166II | GTNNAC | 1 cut(s) | 183 |
| Hpy188III | TCNNGA | 2 cut(s) | 93, 352 |
| Hpy8I | GTNNAC | 1 cut(s) | 183 |
| HpyCH4III | ACNGT | 1 cut(s) | 231 |
| HpyCH4V | TGCA | 6 cut(s) | 78, 168, 226, 262, 359, 385 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 391 |
| HpyF3I | CTNAG | 2 cut(s) | 404, 432 |
| Hsp92II | CATG | 1 cut(s) | 319 |
| Kzo9I | GATC | 1 cut(s) | 95 |
| LmnI | GCTCC | 1 cut(s) | 57 |
| LpnPI | CCDG | 7 cut(s) | 68, 95, 131, 158, 176, 284, 314 |
| LweI | GCATC | 1 cut(s) | 213 |
| MaeIII | GTNAC | 1 cut(s) | 50 |
| MalI | GATC | 1 cut(s) | 97 |
| MboI | GATC | 1 cut(s) | 95 |
| MboII | GAAGA | 5 cut(s) | 58, 194, 270, 380, 401 |
| MhlI | GDGCHC | 1 cut(s) | 48 |
| MluCI | AATT | 7 cut(s) | 174, 209, 257, 288, 307, 346, 372 |
| MlyI | GAGTC | 1 cut(s) | 280 |
| MnlI | CCTC | 5 cut(s) | 20, 138, 208, 323, 384 |
| MseI | TTAA | 1 cut(s) | 158 |
| MspA1I | CMGCKG | 1 cut(s) | 447 |
| MspI | CCGG | 1 cut(s) | 55 |
| MspR9I | CCNGG | 1 cut(s) | 146 |
| MvaI | CCWGG | 1 cut(s) | 146 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 391 |
| NdeII | GATC | 1 cut(s) | 95 |
| NlaIII | CATG | 1 cut(s) | 319 |
| NmuCI | GTSAC | 1 cut(s) | 50 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 39 |
| PfeI | GAWTC | 3 cut(s) | 187, 379, 428 |
| PkrI | GCNGC | 1 cut(s) | 446 |
| PleI | GAGTC | 1 cut(s) | 279 |
| PpsI | GAGTC | 1 cut(s) | 279 |
| Psp124BI | GAGCTC | 1 cut(s) | 48 |
| Psp6I | CCWGG | 1 cut(s) | 144 |
| PspEI | GGTNACC | 1 cut(s) | 50 |
| PspGI | CCWGG | 1 cut(s) | 144 |
| PvuII | CAGCTG | 1 cut(s) | 447 |
| RsaI | GTAC | 3 cut(s) | 182, 252, 320 |
| RsaNI | GTAC | 3 cut(s) | 181, 251, 319 |
| SacI | GAGCTC | 1 cut(s) | 48 |
| SaqAI | TTAA | 1 cut(s) | 158 |
| SatI | GCNGC | 1 cut(s) | 445 |
| Sau3AI | GATC | 1 cut(s) | 95 |
| SchI | GAGTC | 1 cut(s) | 280 |
| ScrFI | CCNGG | 1 cut(s) | 146 |
| SduI | GDGCHC | 1 cut(s) | 48 |
| SetI | ASST | 8 cut(s) | 48, 62, 87, 130, 165, 333, 438, 449 |
| SfaNI | GCATC | 1 cut(s) | 213 |
| Sse9I | AATT | 7 cut(s) | 174, 209, 257, 288, 307, 346, 372 |
| SstI | GAGCTC | 1 cut(s) | 48 |
| StyD4I | CCNGG | 1 cut(s) | 144 |
| TaaI | ACNGT | 1 cut(s) | 231 |
| TaqI | TCGA | 1 cut(s) | 213 |
| TasI | AATT | 7 cut(s) | 174, 209, 257, 288, 307, 346, 372 |
| TatI | WGTACW | 2 cut(s) | 180, 318 |
| TfiI | GAWTC | 3 cut(s) | 187, 379, 428 |
| Tru1I | TTAA | 1 cut(s) | 158 |
| Tru9I | TTAA | 1 cut(s) | 158 |
| TscAI | CASTG | 1 cut(s) | 105 |
| TseFI | GTSAC | 1 cut(s) | 50 |
| TseI | GCWGC | 1 cut(s) | 444 |
| Tsp45I | GTSAC | 1 cut(s) | 50 |
| TspDTI | ATGAA | 3 cut(s) | 58, 381, 441 |
| TspRI | CASTG | 1 cut(s) | 105 |
| XapI | RAATTY | 1 cut(s) | 307 |