Rh2CG648600

Protein of unknown function (DUF3754)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000718
Physical Location & Seq
Forward (+)
81720032 .. 81725177
5146 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG648600.1

Sequence Viewer

Length: 1428 bp
ATGAACATAGAAAATGCCAGTTCTGCACTTTTTATCTCTCCAGAGCAAAGTTCTGATCGGACTGAGTTTTTGAAGCTATGCAAGAGGGTCGAGTACACAATTCGGGCTTGGTATCTTCTACAATTTGAGGATTTGATGCAACTGTACTCCCTCTTTGATCCTGTACATGGAGCCCAGAGGTTGGAGCAACAGAAACTTGACGATGAAGAAATTGATGTGCTTGAACAAAATTTCCTCATGTACTTATTTCAGGTGATGGAAAAGAGTAATTTTAAGATTGCAAACAATGAAGAAATTGAGGTTGCGGCTTCAGGGCAGTATCTTCTACGTCTTCCCATCGTGGTTGATGAGTCTAAGCTTGACAACAGACTTTTGAAGAAATATTTCGAAGGTCATCCTACCGAAGACATTCCAGATTTTGCTGATAAGTATGTCATCTTCCGACGCGGCATTGGACTTGATAAGACAACCGGTTGGTTTTTCATGGAGAAGGTGGACATTATCATTGCACGTTTTTGGAAATATCTTTTACAAATAACAAAGTTAGACAAGTTTTTCAAGAAACCAACCCCTAAGAAGAGGGATTCAGAATCTGATACAGATGAGGACGACTTCTTTGTTGAACGAATCCGTCTTGAAAACATGGGACTAAGCGTTCAAACTTTGCTTCTTAAGAACACAATCCAGGAGCCAACTTTTGAAAGGATTATTGTTGTTTACAGACAAGCAACTACCGACGAGGATGGTTCAGAACGGGGAATACATATTAAACATTTCAAAAATATTCCAATGGCTGATTTGGAGATAGTTCTTCCTGAAAAGAAGAACCCAGGTTTAACACCAATGGACTGGCTCTCGTTCATTGGCTCTGCTGTAGTTGGTCTGGTTGCTGTTATTACTTCGATTGAAACTACTAAAGTCGATCTTTGGGTTATGTTTGCGATTCTTTCCACTGTGATTGGTTACTGTGCTAAGACATATTTCAGTTTTCAGCAGAACTTAGCTCAATATCAGAACTTGATAACACAATCCATGTACGAAAAACAACTAGATAGTGGAAGGGGAACTCTTCTTCACTTGTGTGATGATGTGATTCAACAGGAAGTCAAGGAAGTAATAGTTTCTTTCTATATGTTAATGGAACAAGGGAAAGCTACTTTACCTGATCTGGACAAGTGGTGTGAGGACCTAATCAAAGAACAGTTTAACGACGATATCAATTTTGATGTGGACGATGCAGTTAAGAAGTTAGAGAAATTGGGAATTGTTACTCGGGATACCCTTGGACGGTATAGCGCTGTTGGGCTGAAACGTGCTAACGAGATCATTGGCACCACCACTGAGGAGGTTGTCCTCAAGGCAAAACAAGATGCTGCTAATGTTCCTGCTTCTTCTGCAGCTGCTGCGGTTGCTGCCGCTGGTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3754

Protein Analysis

475

Amino Acids

54.48

Weight (kDa)

4.87

Isoelectric Point (pI)

33.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3754 PF12576 233 - 355 2e-24 Protein of unknown function (DUF3754)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1331
AccB7I CCANNNNNTGG 1 cut(s) 181
AccII CGCG 1 cut(s) 447
AciI CCGC 4 cut(s) 305, 447, 1406, 1416
AclWI GGATC 1 cut(s) 152
AcsI RAATTY 1 cut(s) 229
AcuI CTGAAG 1 cut(s) 294
AfaI GTAC 5 cut(s) 95, 146, 165, 242, 1037
AfeI AGCGCT 1 cut(s) 1297
AfiI CCNNNNNNNGG 3 cut(s) 167, 181, 1287
AflII CTTAAG 1 cut(s) 671
AgeI ACCGGT 1 cut(s) 470
AjnI CCWGG 2 cut(s) 684, 829
AleI CACNNNNGTG 1 cut(s) 1080
AloI GAACNNNNNNTCC 4 cut(s) 216, 248, 639, 671
AluBI AGCT 5 cut(s) 76, 358, 1004, 1154, 1400
AluI AGCT 5 cut(s) 76, 358, 1004, 1154, 1400
AlwI GGATC 1 cut(s) 152
AlwNI CAGNNNCTG 2 cut(s) 593, 1403
Ama87I CYCGRG 1 cut(s) 1272
Aor51HI AGCGCT 1 cut(s) 1297
ApeKI GCWGC 5 cut(s) 1373, 1397, 1400, 1403, 1412
ApoI RAATTY 1 cut(s) 229
ArsI GACNNNNNNTTYG 4 cut(s) 52, 84, 599, 631
AsiGI ACCGGT 1 cut(s) 470
Asp700I GAANNNNTTC 2 cut(s) 383, 408
AspLEI GCGC 1 cut(s) 1298
AspS9I GGNCC 1 cut(s) 1186
AsuHPI GGTGA 1 cut(s) 265
AsuII TTCGAA 1 cut(s) 387
AvaI CYCGRG 1 cut(s) 1272
AvaII GGWCC 1 cut(s) 1186
BanI GGYRCC 1 cut(s) 1331
BanII GRGCYC 1 cut(s) 175
BarI GAAGNNNNNNTAC 2 cut(s) 422, 454
BbsI GAAGAC 2 cut(s) 323, 411
BbvI GCAGC 5 cut(s) 1360, 1387, 1390, 1399, 1409
BccI CCATC 3 cut(s) 250, 344, 737
BcgI CGANNNNNNTGC 2 cut(s) 70, 104
BciT130I CCWGG 2 cut(s) 686, 831
BciVI GTATCC 1 cut(s) 1270
BfaI CTAG 1 cut(s) 1049
BfmI CTRYAG 2 cut(s) 873, 1395
BfoI RGCGCY 1 cut(s) 1299
BfrI CTTAAG 1 cut(s) 671
BfuI GTATCC 1 cut(s) 1270
BisI GCNGC 8 cut(s) 306, 448, 1374, 1398, 1401, 1404, 1413, 1416
BlsI GCNGC 8 cut(s) 307, 449, 1375, 1399, 1402, 1405, 1414, 1417
Bme1390I CCNGG 2 cut(s) 686, 831
Bme18I GGWCC 1 cut(s) 1186
BmeT110I CYCGRG 1 cut(s) 1272
BmgT120I GGNCC 1 cut(s) 1186
BmiI GGNNCC 3 cut(s) 172, 690, 1333
BmrFI CCNGG 2 cut(s) 686, 831
BmsI GCATC 3 cut(s) 126, 1225, 1360
BpiI GAAGAC 2 cut(s) 323, 411
BpmI CTGGAG 1 cut(s) 24
Bpu14I TTCGAA 1 cut(s) 387
BpuEI CTTGAG 1 cut(s) 1340
BsaJI CCNNGG 2 cut(s) 829, 1282
BsaWI WCCGGW 1 cut(s) 470
Bsc4I CCNNNNNNNGG 3 cut(s) 167, 181, 1287
Bse118I RCCGGY 1 cut(s) 470
Bse1I ACTGG 2 cut(s) 18, 854
Bse3DI GCAATG 1 cut(s) 504
BseBI CCWGG 2 cut(s) 686, 831
BseDI CCNNGG 2 cut(s) 829, 1282
BseGI GGATG 2 cut(s) 394, 748
BseLI CCNNNNNNNGG 3 cut(s) 167, 181, 1287
BseMI GCAATG 1 cut(s) 504
BseMII CTCAG 2 cut(s) 54, 1332
BseNI ACTGG 2 cut(s) 18, 854
BseRI GAGGAG 1 cut(s) 1358
BseXI GCAGC 5 cut(s) 1360, 1387, 1390, 1399, 1409
BsgI GTGCAG 1 cut(s) 9
Bsh1236I CGCG 1 cut(s) 447
BshNI GGYRCC 1 cut(s) 1331
BshTI ACCGGT 1 cut(s) 470
BsiHKCI CYCGRG 1 cut(s) 1272
BsiSI CCGG 1 cut(s) 471
BslFI GGGAC 1 cut(s) 660
BslI CCNNNNNNNGG 3 cut(s) 167, 181, 1287
BsmFI GGGAC 1 cut(s) 660
BsoBI CYCGRG 1 cut(s) 1272
Bsp119I TTCGAA 1 cut(s) 387
Bsp1286I GDGCHC 1 cut(s) 175
Bsp1407I TGTACA 1 cut(s) 163
Bsp143I GATC 5 cut(s) 55, 157, 922, 1165, 1323
BspACI CCGC 4 cut(s) 305, 447, 1406, 1416
BspCNI CTCAG 2 cut(s) 55, 1333
BspFNI CGCG 1 cut(s) 447
BspLI GGNNCC 3 cut(s) 172, 690, 1333
BspMAI CTGCAG 1 cut(s) 1399
BspPI GGATC 1 cut(s) 152
BspT104I TTCGAA 1 cut(s) 387
BspT107I GGYRCC 1 cut(s) 1331
BspTI CTTAAG 1 cut(s) 671
BsrDI GCAATG 1 cut(s) 504
BsrFI RCCGGY 1 cut(s) 470
BsrGI TGTACA 1 cut(s) 163
BsrI ACTGG 2 cut(s) 18, 854
BssAI RCCGGY 1 cut(s) 470
BssECI CCNNGG 2 cut(s) 829, 1282
BssMI GATC 5 cut(s) 55, 157, 922, 1165, 1323
BssT1I CCWWGG 1 cut(s) 1282
Bst2UI CCWGG 2 cut(s) 686, 831
Bst4CI ACNGT 5 cut(s) 144, 955, 968, 1203, 1290
Bst6I CTCTTC 2 cut(s) 572, 1074
BstAFI CTTAAG 1 cut(s) 671
BstAPI GCANNNNNTGC 1 cut(s) 1403
BstAUI TGTACA 1 cut(s) 163
BstBI TTCGAA 1 cut(s) 387
BstDEI CTNAG 7 cut(s) 63, 354, 573, 650, 972, 1000, 1341
BstF5I GGATG 2 cut(s) 394, 748
BstFNI CGCG 1 cut(s) 447
BstH2I RGCGCY 1 cut(s) 1299
BstHHI GCGC 1 cut(s) 1298
BstKTI GATC 5 cut(s) 58, 160, 925, 1168, 1326
BstMBI GATC 5 cut(s) 55, 157, 922, 1165, 1323
BstMWI GCNNNNNNNGC 5 cut(s) 23, 1394, 1403, 1409, 1412
BstNI CCWGG 2 cut(s) 686, 831
BstSCI CCNGG 2 cut(s) 684, 829
BstSFI CTRYAG 2 cut(s) 873, 1395
BstUI CGCG 1 cut(s) 447
BstV1I GCAGC 5 cut(s) 1360, 1387, 1390, 1399, 1409
BstV2I GAAGAC 2 cut(s) 323, 411
BstXI CCANNNNNNTGG 1 cut(s) 849
BsuI GTATCC 1 cut(s) 1270
BtsCI GGATG 2 cut(s) 394, 748
BtsIMutI CAGTG 2 cut(s) 951, 1338
CaiI CAGNNNCTG 2 cut(s) 593, 1403
CfoI GCGC 1 cut(s) 1298
Cfr10I RCCGGY 1 cut(s) 470
Cfr13I GGNCC 1 cut(s) 1186
CseI GACGC 1 cut(s) 453
Csp6I GTAC 5 cut(s) 94, 145, 164, 241, 1036
CspAI ACCGGT 1 cut(s) 470
CspCI CAANNNNNGTGG 2 cut(s) 940, 975
CviAII CATG 5 cut(s) 167, 238, 484, 643, 1033
CviQI GTAC 5 cut(s) 94, 145, 164, 241, 1036
DdeI CTNAG 7 cut(s) 63, 354, 573, 650, 972, 1000, 1341
DpnI GATC 5 cut(s) 57, 159, 924, 1167, 1325
DpnII GATC 5 cut(s) 55, 157, 922, 1165, 1323
Eam1104I CTCTTC 2 cut(s) 572, 1074
EarI CTCTTC 2 cut(s) 572, 1074
Eco130I CCWWGG 1 cut(s) 1282
Eco24I GRGCYC 1 cut(s) 175
Eco32I GATATC 1 cut(s) 1216
Eco47I GGWCC 1 cut(s) 1186
Eco47III AGCGCT 1 cut(s) 1297
Eco57I CTGAAG 1 cut(s) 294
Eco88I CYCGRG 1 cut(s) 1272
EcoO109I RGGNCCY 1 cut(s) 1186
EcoRII CCWGG 2 cut(s) 684, 829
EcoRV GATATC 1 cut(s) 1216
EcoT14I CCWWGG 1 cut(s) 1282
EcoT38I GRGCYC 1 cut(s) 175
ErhI CCWWGG 1 cut(s) 1282
FaeI CATG 5 cut(s) 170, 241, 487, 646, 1036
FalI AAGNNNNNCTT 2 cut(s) 909, 941
FaqI GGGAC 1 cut(s) 660
FatI CATG 5 cut(s) 166, 237, 483, 642, 1032
Fnu4HI GCNGC 8 cut(s) 306, 448, 1374, 1398, 1401, 1404, 1413, 1416
FokI GGATG 2 cut(s) 381, 755
FriOI GRGCYC 1 cut(s) 175
Fsp4HI GCNGC 8 cut(s) 306, 448, 1374, 1398, 1401, 1404, 1413, 1416
FspBI CTAG 1 cut(s) 1049
GlaI GCGC 1 cut(s) 1297
GluI GCNGC 8 cut(s) 306, 448, 1374, 1398, 1401, 1404, 1413, 1416
GsuI CTGGAG 1 cut(s) 24
HaeII RGCGCY 1 cut(s) 1299
HapII CCGG 1 cut(s) 471
HgaI GACGC 1 cut(s) 453
HhaI GCGC 1 cut(s) 1298
Hin1II CATG 5 cut(s) 170, 241, 487, 646, 1036
Hin6I GCGC 1 cut(s) 1296
HinP1I GCGC 1 cut(s) 1296
HindIII AAGCTT 1 cut(s) 356
HinfI GANTC 6 cut(s) 350, 584, 590, 627, 943, 1093
HpaII CCGG 1 cut(s) 471
HphI GGTGA 1 cut(s) 265
Hpy166II GTNNAC 4 cut(s) 96, 496, 718, 1231
Hpy188I TCNGA 7 cut(s) 55, 60, 443, 589, 595, 751, 1014
Hpy188III TCNNGA 7 cut(s) 41, 413, 559, 635, 815, 1169, 1274
Hpy8I GTNNAC 4 cut(s) 96, 496, 718, 1231
Hpy99I CGWCG 3 cut(s) 447, 740, 1214
HpyAV CCTTC 3 cut(s) 383, 484, 1053
HpyCH4III ACNGT 5 cut(s) 144, 955, 968, 1203, 1290
HpyCH4IV ACGT 3 cut(s) 328, 511, 1312
HpyCH4V TGCA 7 cut(s) 26, 81, 139, 281, 509, 1238, 1397
HpyF10VI GCNNNNNNNGC 5 cut(s) 23, 1394, 1403, 1409, 1412
HpyF3I CTNAG 7 cut(s) 63, 354, 573, 650, 972, 1000, 1341
HpySE526I ACGT 3 cut(s) 328, 511, 1312
Hsp92II CATG 5 cut(s) 170, 241, 487, 646, 1036
HspAI GCGC 1 cut(s) 1296
Kzo9I GATC 5 cut(s) 55, 157, 922, 1165, 1323
LmnI GCTCC 3 cut(s) 170, 184, 688
Lsp1109I GCAGC 5 cut(s) 1360, 1387, 1390, 1399, 1409
LweI GCATC 3 cut(s) 126, 1225, 1360
MaeI CTAG 1 cut(s) 1049
MaeII ACGT 3 cut(s) 328, 511, 1312
MaeIII GTNAC 2 cut(s) 962, 1267
MalI GATC 5 cut(s) 57, 159, 924, 1167, 1325
MboI GATC 5 cut(s) 55, 157, 922, 1165, 1323
MhlI GDGCHC 1 cut(s) 175
MluCI AATT 9 cut(s) 99, 122, 210, 229, 268, 294, 1219, 1256, 1263
MlyI GAGTC 1 cut(s) 359
MmeI TCCRAC 2 cut(s) 162, 466
MroXI GAANNNNTTC 2 cut(s) 383, 408
MseI TTAA 8 cut(s) 273, 672, 768, 836, 1136, 1206, 1242, 1426
MslI CAYNNNNRTG 1 cut(s) 1080
MspA1I CMGCKG 2 cut(s) 1400, 1418
MspCI CTTAAG 1 cut(s) 671
MspI CCGG 1 cut(s) 471
MspR9I CCNGG 2 cut(s) 686, 831
MvaI CCWGG 2 cut(s) 686, 831
MvnI CGCG 1 cut(s) 447
MwoI GCNNNNNNNGC 5 cut(s) 23, 1394, 1403, 1409, 1412
NdeII GATC 5 cut(s) 55, 157, 922, 1165, 1323
NlaIII CATG 5 cut(s) 170, 241, 487, 646, 1036
NlaIV GGNNCC 3 cut(s) 172, 690, 1333
NspV TTCGAA 1 cut(s) 387
OliI CACNNNNGTG 1 cut(s) 1080
PdmI GAANNNNTTC 2 cut(s) 383, 408
PfeI GAWTC 5 cut(s) 584, 590, 627, 943, 1093
PflMI CCANNNNNTGG 1 cut(s) 181
PfoI TCCNGGA 1 cut(s) 684
PinAI ACCGGT 1 cut(s) 470
PkrI GCNGC 8 cut(s) 307, 449, 1375, 1399, 1402, 1405, 1414, 1417
PleI GAGTC 1 cut(s) 358
PpsI GAGTC 1 cut(s) 358
PpuMI RGGWCCY 1 cut(s) 1186
Psp5II RGGWCCY 1 cut(s) 1186
Psp6I CCWGG 2 cut(s) 684, 829
PspGI CCWGG 2 cut(s) 684, 829
PspN4I GGNNCC 3 cut(s) 172, 690, 1333
PspPI GGNCC 1 cut(s) 1186
PspPPI RGGWCCY 1 cut(s) 1186
PsrI GAACNNNNNNTAC 2 cut(s) 744, 776
PstI CTGCAG 1 cut(s) 1399
PstNI CAGNNNCTG 2 cut(s) 593, 1403
PvuII CAGCTG 1 cut(s) 1400
RsaI GTAC 5 cut(s) 95, 146, 165, 242, 1037
RsaNI GTAC 5 cut(s) 94, 145, 164, 241, 1036
RseI CAYNNNNRTG 1 cut(s) 1080
SaqAI TTAA 8 cut(s) 273, 672, 768, 836, 1136, 1206, 1242, 1426
SatI GCNGC 8 cut(s) 306, 448, 1374, 1398, 1401, 1404, 1413, 1416
Sau3AI GATC 5 cut(s) 55, 157, 922, 1165, 1323
Sau96I GGNCC 1 cut(s) 1186
SchI GAGTC 1 cut(s) 359
ScrFI CCNGG 2 cut(s) 686, 831
SduI GDGCHC 1 cut(s) 175
SfaNI GCATC 3 cut(s) 126, 1225, 1360
SfcI CTRYAG 2 cut(s) 873, 1395
SfuI TTCGAA 1 cut(s) 387
SinI GGWCC 1 cut(s) 1186
SmiMI CAYNNNNRTG 1 cut(s) 1080
SmlI CTYRAG 2 cut(s) 671, 1355
SmoI CTYRAG 2 cut(s) 671, 1355
Sse9I AATT 9 cut(s) 99, 122, 210, 229, 268, 294, 1219, 1256, 1263
SsiI CCGC 4 cut(s) 305, 447, 1406, 1416
SspI AATATT 2 cut(s) 383, 784
SspMI CTAG 1 cut(s) 1049
StyD4I CCNGG 2 cut(s) 684, 829
StyI CCWWGG 1 cut(s) 1282
TaaI ACNGT 5 cut(s) 144, 955, 968, 1203, 1290
TaiI ACGT 3 cut(s) 331, 514, 1315
TaqI TCGA 4 cut(s) 90, 387, 902, 921
TasI AATT 9 cut(s) 99, 122, 210, 229, 268, 294, 1219, 1256, 1263
TatI WGTACW 4 cut(s) 93, 144, 163, 240
TauI GCSGC 3 cut(s) 308, 450, 1418
TfiI GAWTC 5 cut(s) 584, 590, 627, 943, 1093
Tru1I TTAA 8 cut(s) 273, 672, 768, 836, 1136, 1206, 1242, 1426
Tru9I TTAA 8 cut(s) 273, 672, 768, 836, 1136, 1206, 1242, 1426
TscAI CASTG 2 cut(s) 958, 1345
TseI GCWGC 5 cut(s) 1373, 1397, 1400, 1403, 1412
TspDTI ATGAA 5 cut(s) 17, 219, 303, 472, 850
TspGWI ACGGA 1 cut(s) 620
TspRI CASTG 2 cut(s) 958, 1345
Van91I CCANNNNNTGG 1 cut(s) 181
Vha464I CTTAAG 1 cut(s) 671
VpaK11BI GGWCC 1 cut(s) 1186
XapI RAATTY 1 cut(s) 229
XmnI GAANNNNTTC 2 cut(s) 383, 408
XspI CTAG 1 cut(s) 1049
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.