Rw2G051880

Protein of unknown function (DUF3754)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000718
Physical Location & Seq
Reverse (-)
79680180 .. 79684702
4523 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G051880.1

Sequence Viewer

Length: 1476 bp
ATGGAAAAAACCGCGTCCCAGAAGAAGAAAGACATGATTCGAATAGAGCGCGAGTCCGTGATTCCGGTTCTCAAGCCCAAGCTCATCATGTCCTTGGCCAACCTCATTGAACATAGTTCCGACCGGGCGGAGTTTTTAAAGCTCTGCAAGAGAATTGAGTACACGATTCGGGCTTGGTATCTGCTACAATTTGAGGATTTGATGCAACTGTATTCGCTATTTGACCCCGTACATGGGAATCAAAAATTGGAGCAACAGAATCTTTCTCCTAAAGAGATTGATGTACTGGAACAGAATTTCCTCACGTACTTACTTCAGGTGATGGAAAAGAGTAATTTCAAGATTGCAACTGATGAAGAAATTGAGATTGCACATTCGGGGCAGTATCTTCTAAATCTTCCCATCGTGGTTGATGAATCTAAGATTGACAAGAAGCTTCTGAAGAACTTCTTTGCAGAGAATCCTCAGCAAAACCTTCCAGATTTTGCTGATAAGTACATTATCTTCCGGCGTGGTGTTGGACTTGATCAGACAACTGATTACTTTTTCATGGAGAAAGTGGACATGATCATTGCACGTTTTTGGGCATATCTTTTACGAATAACTAGGGCTGAAAAACTTTTATCCAGAAGGTCAAGTAGTCGGCACACAAAAGATCCAAAGAAGGATGATGAAATTACCTCAGAAGCAGATGGGGAGGAGGCGGGGGACTTATACGTTGAACGAATTCGTCTTGAAAATATGGAACTAAGCGTTAAAAGCTTGCTGAGCAAGACCACAATCAAAGAGCCAACCTTTGATAGGATAATTGTTGTTTACAGGCGAGCAAGTACCAAAGCAAAAGAAGAACGAGGAATATTTGTGAAACATTTCAAAAATATTCCCATGGCTGATATGGAGATAGTTCTTCCCGAGAAGAAGAACCCAGGATTAACTCCAATGGACTGGGTCAAGTTCCTTGGCTCTGCTGTAGTTGGGCTGGTTGCTGTTATTAGTTCGCTTGAAATGCCTAATGCTGACTTTTGGGTCATCATTGCGGTTCTTTCCACTGTCATTGGTTACTGTGCTAAGATATACTTCAGTTTTCAGCAGAACTTGGCTGCATATCAGAACCTAATAACACAGTCCATGTATGACAAGCAACTGGATAGCGGAAGGGGTACTCTTCTTCACTTGTGTGATGATGTGATTCAACAGGAGGTCAAGGAGGTGATACTTTCTTTCTATATATTAATGGAACAGGGAAAAGCTACAAGAGAAGATCTGGATAGGTGGTGTGAGGAACTCATTAAAGAAGAGTTTGTGCGAGTTGTTAATTTTGATGTGGTGATGCAGTTGGGAAGTTGGACGGCTTCTTTCTTATCTTTACTAGAGGGTGTTTATTTCTTGGGGCAGGTGCTAGACCATCATCAATCCTCTTTGATGGAAGATTATCCTCAAATATTTCGCTGCTTGTTAATATCTCTAAATCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3754

Protein Analysis

491

Amino Acids

57.03

Weight (kDa)

5.46

Isoelectric Point (pI)

36.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3754 PF12576 266 - 387 7.1e-23 Protein of unknown function (DUF3754)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1384
AasI GACNNNNNNGTC 1 cut(s) 1025
Acc36I ACCTGC 1 cut(s) 1384
AccII CGCG 2 cut(s) 14, 51
AciI CCGC 5 cut(s) 12, 128, 704, 1037, 1152
AclWI GGATC 1 cut(s) 650
AcoI YGGCCR 1 cut(s) 96
AcsI RAATTY 2 cut(s) 295, 726
AcuI CTGAAG 3 cut(s) 299, 461, 1063
AfaI GTAC 7 cut(s) 161, 231, 285, 308, 497, 832, 1162
AfiI CCNNNNNNNGG 3 cut(s) 233, 234, 801
AgsI TTSAA 7 cut(s) 110, 340, 722, 737, 874, 1004, 1193
AjnI CCWGG 1 cut(s) 925
AjuI GAANNNNNNNTTGG 2 cut(s) 230, 262
AleI CACNNNNGTG 1 cut(s) 1176
AloI GAACNNNNNNTCC 2 cut(s) 282, 314
AluBI AGCT 5 cut(s) 82, 142, 436, 762, 1250
AluI AGCT 5 cut(s) 82, 142, 436, 762, 1250
AlwI GGATC 1 cut(s) 650
Ama87I CYCGRG 1 cut(s) 911
AoxI GGCC 1 cut(s) 96
ApeKI GCWGC 2 cut(s) 1100, 1449
ApoI RAATTY 2 cut(s) 295, 726
AseI ATTAAT 1 cut(s) 1232
Asp700I GAANNNNTTC 3 cut(s) 446, 726, 869
AspLEI GCGC 1 cut(s) 51
AsuC2I CCSGG 1 cut(s) 125
AsuHPI GGTGA 3 cut(s) 331, 1222, 1339
AsuII TTCGAA 1 cut(s) 40
AvaI CYCGRG 1 cut(s) 911
BalI TGGCCA 1 cut(s) 98
BarI GAAGNNNNNNTAC 2 cut(s) 488, 520
BbvCI CCTCAGC 1 cut(s) 465
BbvI GCAGC 2 cut(s) 1087, 1436
BccI CCATC 5 cut(s) 316, 410, 686, 1413, 1417
BceAI ACGGC 1 cut(s) 1365
BciT130I CCWGG 1 cut(s) 927
BclI TGATCA 2 cut(s) 526, 567
BcnI CCSGG 1 cut(s) 125
BfaI CTAG 3 cut(s) 606, 1370, 1400
BfmI CTRYAG 1 cut(s) 969
BfuAI ACCTGC 1 cut(s) 1384
BglII AGATCT 1 cut(s) 1261
BisI GCNGC 2 cut(s) 1101, 1450
BlpI GCTNAGC 1 cut(s) 767
BlsI GCNGC 2 cut(s) 1102, 1451
Bme1390I CCNGG 2 cut(s) 125, 927
BmeT110I CYCGRG 1 cut(s) 911
BmrFI CCNGG 2 cut(s) 125, 927
BmrI ACTGGG 1 cut(s) 955
BmsI GCATC 2 cut(s) 192, 1320
BmuI ACTGGG 1 cut(s) 955
Bpu10I CCTNAGC 1 cut(s) 465
Bpu1102I GCTNAGC 1 cut(s) 767
Bpu14I TTCGAA 1 cut(s) 40
BpuEI CTTGAG 1 cut(s) 56
BpuMI CCSGG 1 cut(s) 125
BsaAI YACGTR 1 cut(s) 306
BsaJI CCNNGG 4 cut(s) 93, 885, 925, 958
BsaWI WCCGGW 1 cut(s) 64
Bsc4I CCNNNNNNNGG 3 cut(s) 233, 234, 801
Bse1I ACTGG 3 cut(s) 291, 950, 1149
Bse3DI GCAATG 2 cut(s) 570, 1032
BseBI CCWGG 1 cut(s) 927
BseDI CCNNGG 4 cut(s) 93, 885, 925, 958
BseGI GGATG 1 cut(s) 673
BseLI CCNNNNNNNGG 3 cut(s) 233, 234, 801
BseMI GCAATG 2 cut(s) 570, 1032
BseMII CTCAG 3 cut(s) 479, 696, 758
BseNI ACTGG 3 cut(s) 291, 950, 1149
BseRI GAGGAG 1 cut(s) 713
BseXI GCAGC 2 cut(s) 1087, 1436
Bsh1236I CGCG 2 cut(s) 14, 51
Bsh1285I CGRYCG 1 cut(s) 124
BshFI GGCC 1 cut(s) 98
BsiEI CGRYCG 1 cut(s) 124
BsiHKCI CYCGRG 1 cut(s) 911
BsiSI CCGG 3 cut(s) 65, 124, 508
BslFI GGGAC 1 cut(s) 722
BslI CCNNNNNNNGG 3 cut(s) 233, 234, 801
BsmFI GGGAC 1 cut(s) 722
BsnI GGCC 1 cut(s) 98
BsoBI CYCGRG 1 cut(s) 911
Bsp119I TTCGAA 1 cut(s) 40
Bsp143I GATC 4 cut(s) 526, 567, 655, 1261
Bsp1720I GCTNAGC 1 cut(s) 767
Bsp19I CCATGG 1 cut(s) 885
BspACI CCGC 5 cut(s) 12, 128, 704, 1037, 1152
BspANI GGCC 1 cut(s) 98
BspCNI CTCAG 3 cut(s) 478, 695, 759
BspFNI CGCG 2 cut(s) 14, 51
BspMI ACCTGC 1 cut(s) 1384
BspPI GGATC 1 cut(s) 650
BspT104I TTCGAA 1 cut(s) 40
BsrDI GCAATG 2 cut(s) 570, 1032
BsrI ACTGG 3 cut(s) 291, 950, 1149
BssECI CCNNGG 4 cut(s) 93, 885, 925, 958
BssMI GATC 4 cut(s) 526, 567, 655, 1261
BssT1I CCWWGG 3 cut(s) 93, 885, 958
Bst2UI CCWGG 1 cut(s) 927
Bst4CI ACNGT 4 cut(s) 210, 1051, 1064, 1125
Bst6I CTCTTC 2 cut(s) 1170, 1290
BstBAI YACGTR 1 cut(s) 306
BstBI TTCGAA 1 cut(s) 40
BstC8I GCNNGC 2 cut(s) 764, 825
BstDEI CTNAG 6 cut(s) 420, 465, 682, 749, 767, 1068
BstDSI CCRYGG 1 cut(s) 885
BstENI CCTNNNNNAGG 1 cut(s) 799
BstF5I GGATG 1 cut(s) 673
BstFNI CGCG 2 cut(s) 14, 51
BstHHI GCGC 1 cut(s) 51
BstKTI GATC 4 cut(s) 529, 570, 658, 1264
BstMBI GATC 4 cut(s) 526, 567, 655, 1261
BstMCI CGRYCG 1 cut(s) 124
BstMWI GCNNNNNNNGC 3 cut(s) 759, 768, 1006
BstNI CCWGG 1 cut(s) 927
BstSCI CCNGG 2 cut(s) 123, 925
BstSFI CTRYAG 1 cut(s) 969
BstUI CGCG 2 cut(s) 14, 51
BstV1I GCAGC 2 cut(s) 1087, 1436
BstX2I RGATCY 2 cut(s) 655, 1261
BstXI CCANNNNNNTGG 1 cut(s) 945
BstYI RGATCY 2 cut(s) 655, 1261
BsuRI GGCC 1 cut(s) 98
BtgI CCRYGG 1 cut(s) 885
BtsCI GGATG 1 cut(s) 673
BtsIMutI CAGTG 1 cut(s) 1047
BveI ACCTGC 1 cut(s) 1384
Cac8I GCNNGC 2 cut(s) 764, 825
CfoI GCGC 1 cut(s) 51
CseI GACGC 1 cut(s) 3
Csp6I GTAC 7 cut(s) 160, 230, 284, 307, 496, 831, 1161
CspCI CAANNNNNGTGG 2 cut(s) 1036, 1071
CviAII CATG 7 cut(s) 34, 88, 233, 550, 565, 886, 1129
CviQI GTAC 7 cut(s) 160, 230, 284, 307, 496, 831, 1161
DdeI CTNAG 6 cut(s) 420, 465, 682, 749, 767, 1068
DpnI GATC 4 cut(s) 528, 569, 657, 1263
DpnII GATC 4 cut(s) 526, 567, 655, 1261
DraI TTTAAA 1 cut(s) 138
DrdI GACNNNNNNGTC 1 cut(s) 1025
DseDI GACNNNNNNGTC 1 cut(s) 1025
EaeI YGGCCR 1 cut(s) 96
Eam1104I CTCTTC 2 cut(s) 1170, 1290
EarI CTCTTC 2 cut(s) 1170, 1290
EciI GGCGGA 1 cut(s) 143
Eco130I CCWWGG 3 cut(s) 93, 885, 958
Eco57I CTGAAG 3 cut(s) 299, 461, 1063
Eco88I CYCGRG 1 cut(s) 911
EcoNI CCTNNNNNAGG 1 cut(s) 799
EcoRI GAATTC 1 cut(s) 726
EcoRII CCWGG 1 cut(s) 925
EcoT14I CCWWGG 3 cut(s) 93, 885, 958
ErhI CCWWGG 3 cut(s) 93, 885, 958
FaeI CATG 7 cut(s) 37, 91, 236, 553, 568, 889, 1132
FalI AAGNNNNNCTT 4 cut(s) 434, 466, 1061, 1093
FaqI GGGAC 1 cut(s) 722
FatI CATG 7 cut(s) 33, 87, 232, 549, 564, 885, 1128
FauI CCCGC 1 cut(s) 697
FbaI TGATCA 2 cut(s) 526, 567
Fnu4HI GCNGC 2 cut(s) 1101, 1450
FokI GGATG 1 cut(s) 680
Fsp4HI GCNGC 2 cut(s) 1101, 1450
FspBI CTAG 3 cut(s) 606, 1370, 1400
GlaI GCGC 1 cut(s) 50
GluI GCNGC 2 cut(s) 1101, 1450
HaeIII GGCC 1 cut(s) 98
HapII CCGG 3 cut(s) 65, 124, 508
HgaI GACGC 1 cut(s) 3
HhaI GCGC 1 cut(s) 51
Hin1II CATG 7 cut(s) 37, 91, 236, 553, 568, 889, 1132
Hin6I GCGC 1 cut(s) 49
HinP1I GCGC 1 cut(s) 49
HindIII AAGCTT 2 cut(s) 434, 760
HinfI GANTC 9 cut(s) 37, 53, 61, 166, 238, 259, 416, 460, 1189
HpaII CCGG 3 cut(s) 65, 124, 508
HphI GGTGA 3 cut(s) 331, 1222, 1339
Hpy166II GTNNAC 3 cut(s) 162, 562, 817
Hpy188I TCNGA 5 cut(s) 121, 441, 531, 685, 1110
Hpy188III TCNNGA 6 cut(s) 340, 479, 627, 734, 911, 1265
Hpy8I GTNNAC 3 cut(s) 162, 562, 817
HpyAV CCTTC 4 cut(s) 485, 624, 658, 1149
HpyCH4III ACNGT 4 cut(s) 210, 1051, 1064, 1125
HpyCH4IV ACGT 3 cut(s) 305, 577, 717
HpyCH4V TGCA 8 cut(s) 147, 205, 347, 371, 455, 575, 1103, 1333
HpyF10VI GCNNNNNNNGC 3 cut(s) 759, 768, 1006
HpyF3I CTNAG 6 cut(s) 420, 465, 682, 749, 767, 1068
HpySE526I ACGT 3 cut(s) 305, 577, 717
Hsp92II CATG 7 cut(s) 37, 91, 236, 553, 568, 889, 1132
HspAI GCGC 1 cut(s) 49
Ksp22I TGATCA 2 cut(s) 526, 567
Kzo9I GATC 4 cut(s) 526, 567, 655, 1261
LmnI GCTCC 1 cut(s) 250
Lsp1109I GCAGC 2 cut(s) 1087, 1436
LweI GCATC 2 cut(s) 192, 1320
MaeI CTAG 3 cut(s) 606, 1370, 1400
MaeII ACGT 3 cut(s) 305, 577, 717
MaeIII GTNAC 1 cut(s) 1058
MalI GATC 4 cut(s) 528, 569, 657, 1263
MboI GATC 4 cut(s) 526, 567, 655, 1261
MflI RGATCY 2 cut(s) 655, 1261
MlsI TGGCCA 1 cut(s) 98
MluNI TGGCCA 1 cut(s) 98
MlyI GAGTC 1 cut(s) 62
MmeI TCCRAC 3 cut(s) 144, 499, 1325
Mox20I TGGCCA 1 cut(s) 98
MroXI GAANNNNTTC 3 cut(s) 446, 726, 869
MscI TGGCCA 1 cut(s) 98
MseI TTAA 7 cut(s) 137, 756, 932, 1232, 1290, 1314, 1457
MslI CAYNNNNRTG 1 cut(s) 1176
Msp20I TGGCCA 1 cut(s) 98
MspI CCGG 3 cut(s) 65, 124, 508
MspR9I CCNGG 2 cut(s) 125, 927
MvaI CCWGG 1 cut(s) 927
MvnI CGCG 2 cut(s) 14, 51
MwoI GCNNNNNNNGC 3 cut(s) 759, 768, 1006
NciI CCSGG 1 cut(s) 125
NcoI CCATGG 1 cut(s) 885
NdeII GATC 4 cut(s) 526, 567, 655, 1261
NlaIII CATG 7 cut(s) 37, 91, 236, 553, 568, 889, 1132
NspV TTCGAA 1 cut(s) 40
OliI CACNNNNGTG 1 cut(s) 1176
PaqCI CACCTGC 1 cut(s) 1384
PdmI GAANNNNTTC 3 cut(s) 446, 726, 869
PfeI GAWTC 8 cut(s) 37, 61, 166, 238, 259, 416, 460, 1189
PflFI GACNNNGTC 1 cut(s) 947
PkrI GCNGC 2 cut(s) 1102, 1451
PleI GAGTC 1 cut(s) 61
PpsI GAGTC 1 cut(s) 61
Ppu21I YACGTR 1 cut(s) 306
PshBI ATTAAT 1 cut(s) 1232
Psp6I CCWGG 1 cut(s) 925
PspGI CCWGG 1 cut(s) 925
PsuI RGATCY 2 cut(s) 655, 1261
PsyI GACNNNGTC 1 cut(s) 947
RsaI GTAC 7 cut(s) 161, 231, 285, 308, 497, 832, 1162
RsaNI GTAC 7 cut(s) 160, 230, 284, 307, 496, 831, 1161
RseI CAYNNNNRTG 1 cut(s) 1176
SaqAI TTAA 7 cut(s) 137, 756, 932, 1232, 1290, 1314, 1457
SatI GCNGC 2 cut(s) 1101, 1450
Sau3AI GATC 4 cut(s) 526, 567, 655, 1261
SchI GAGTC 1 cut(s) 62
ScrFI CCNGG 2 cut(s) 125, 927
SfaNI GCATC 2 cut(s) 192, 1320
SfcI CTRYAG 1 cut(s) 969
SfuI TTCGAA 1 cut(s) 40
SmiMI CAYNNNNRTG 1 cut(s) 1176
SmlI CTYRAG 1 cut(s) 71
SmoI CTYRAG 1 cut(s) 71
SsiI CCGC 5 cut(s) 12, 128, 704, 1037, 1152
SspI AATATT 3 cut(s) 858, 880, 1443
SspMI CTAG 3 cut(s) 606, 1370, 1400
StyD4I CCNGG 2 cut(s) 123, 925
StyI CCWWGG 3 cut(s) 93, 885, 958
TaaI ACNGT 4 cut(s) 210, 1051, 1064, 1125
TaiI ACGT 3 cut(s) 308, 580, 720
TaqI TCGA 1 cut(s) 40
TatI WGTACW 3 cut(s) 159, 283, 495
TfiI GAWTC 8 cut(s) 37, 61, 166, 238, 259, 416, 460, 1189
Tru1I TTAA 7 cut(s) 137, 756, 932, 1232, 1290, 1314, 1457
Tru9I TTAA 7 cut(s) 137, 756, 932, 1232, 1290, 1314, 1457
TscAI CASTG 1 cut(s) 1054
TseI GCWGC 2 cut(s) 1100, 1449
TspDTI ATGAA 4 cut(s) 369, 429, 538, 687
TspGWI ACGGA 1 cut(s) 46
TspRI CASTG 1 cut(s) 1054
Tth111I GACNNNGTC 1 cut(s) 947
VspI ATTAAT 1 cut(s) 1232
XagI CCTNNNNNAGG 1 cut(s) 799
XapI RAATTY 2 cut(s) 295, 726
XcmI CCANNNNNNNNNTGG 1 cut(s) 892
XmnI GAANNNNTTC 3 cut(s) 446, 726, 869
XspI CTAG 3 cut(s) 606, 1370, 1400
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.