Rh2AG626800

Protein of unknown function (DUF3754)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000718
Physical Location & Seq
Reverse (-)
85132164 .. 85137079
4916 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG626800.1

Sequence Viewer

Length: 1488 bp
ATGGAAAAAACCGCGTCCCAGAAGAAGAAAGACATGATTCGAATAGAGCGCGAGTCCGTGATTCCAGTTCTCAAGCCCAAGCTCATCATGTCCTTGGCCAACCTCATTGAACATAGTTCCGACCGGGCGGAGTTTTTAAAGCTCTGCAAGAGAATTGAGTACACGATTCGGGCTTGGTATCTTCTACAATTCGAGGATTTGATGCAACTGTATTCGCTCTTTGACCCCGTACACGGGAATCAAAAATTGGAGCAACAGAATCTTTCTCCTAAAGAGATTGATGTACTGGAACAGAATTTCCTCACGTACTTACTTCAGGTGATGGAAAAGAGTAATTTCAAGATTGCAACTGATGAAGAAATTGAGATTGCACATTCAGGGCAGTATCTTCTAAATCTTCCCATCGTGGTTGATGAATCTAAGATTGACAAGAAGCTTCTGAAGAACTTCTTTGCAGAGAATCCTCAGCAAAACCTTCCAGATTTTGCTGATAAGTACATTATCTTCCGGCGTGGTGTTGGACTTGATCAGACAACTGATTACTTTTTCATGGAGAAAGTGGACATGATCATTGCACGTTTTTGGGCATATCTTTTACGAATAACTAGGGCTGAAAAACTTTTATCCAGAAGGTCAAGTAGTCGGCACACAAAAGATCCAAAGAAGGATGATGAAATTACCTCAGAAGCAGATGGGGAGGAGGCGGAGGACTTATATGTTGAACGAATTCGTCTTGAAAATATGGAACTAAGCGTTAAAAGCTTGCTGAGCAAGACCACAATCAAAGAGCCAACCTTTGATAGGATAATTGTTGTTTACAGGCGAGCAAGTACCAAAGCAAAAGAAGAACGAGGAATATTTGTGAAACATTTCAAAAATATTCCCATGGCTGATATGGAGATAGTTCTTCCCGAGAAGAAGAACCCAGGATTAACTCCAATGGACTGGGTCAAGTTCCTTGGCTCTGCTGTAGTTGGACTGGTTGCTGTTATTAGTTCGCTTGAAATGCCTAATGCTGACTTTTGGGTCATCATTGCGGTTCTTTCCACTGTCATTGGTTACTGTGCTAAGATATACTTCAGTTTTCAGCAGAACTTGGCTGCATATCAGAACCTAATAACACAGTCCATGTATGACAAGCAACTGGATAGCGGAAGGGGTACTCTTCTTCACTTGTGTGATGATGTGATTCAACAGGAGGTCAAGGAGGTGATACTTTCTTTCTATATATTAATGGAACAGGGAAAAGCTACAAGAGAAGATCTGGATAGGTGGTGTGAGGAACTCATTAAAGAAGAGTTTGGTGCCAGTTGTAATTTTGATGTGGATGATGCAGTTGGGAAGTTGGAGAAATTGGGAATTGTCTCTCGGGATTCCGTTGGACGGTATTTCTGCGTTGGGCTGAAACGTGCGAATGAAATAATTGGGACCACCACTGAGGAGCTTGTTATGAGGGCAAAACAAGGCAAACAAGGCAGTAGTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3754

Protein Analysis

495

Amino Acids

57.05

Weight (kDa)

5.6

Isoelectric Point (pI)

36.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3754 PF12576 266 - 387 7.2e-23 Protein of unknown function (DUF3754)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1025
AccB1I GGYRCC 1 cut(s) 1304
AccII CGCG 2 cut(s) 14, 51
AciI CCGC 5 cut(s) 12, 128, 704, 1037, 1152
AclWI GGATC 1 cut(s) 650
AcoI YGGCCR 1 cut(s) 96
AcsI RAATTY 2 cut(s) 295, 726
AcuI CTGAAG 3 cut(s) 299, 461, 1063
AfaI GTAC 7 cut(s) 161, 231, 285, 308, 497, 832, 1162
AfiI CCNNNNNNNGG 4 cut(s) 233, 234, 801, 1383
AgsI TTSAA 7 cut(s) 110, 340, 722, 737, 874, 1004, 1193
AjnI CCWGG 1 cut(s) 925
AjuI GAANNNNNNNTTGG 2 cut(s) 230, 262
AleI CACNNNNGTG 1 cut(s) 1176
AloI GAACNNNNNNTCC 2 cut(s) 282, 314
AluBI AGCT 6 cut(s) 82, 142, 436, 762, 1250, 1444
AluI AGCT 6 cut(s) 82, 142, 436, 762, 1250, 1444
Alw26I GTCTC 1 cut(s) 1369
AlwI GGATC 1 cut(s) 650
Ama87I CYCGRG 2 cut(s) 911, 1368
AoxI GGCC 1 cut(s) 96
ApeKI GCWGC 1 cut(s) 1100
ApoI RAATTY 2 cut(s) 295, 726
AseI ATTAAT 1 cut(s) 1232
Asp700I GAANNNNTTC 3 cut(s) 446, 726, 869
AspLEI GCGC 1 cut(s) 51
AspS9I GGNCC 1 cut(s) 1428
AsuC2I CCSGG 1 cut(s) 125
AsuHPI GGTGA 2 cut(s) 331, 1222
AsuII TTCGAA 1 cut(s) 40
AvaI CYCGRG 2 cut(s) 911, 1368
AvaII GGWCC 1 cut(s) 1428
BalI TGGCCA 1 cut(s) 98
BanI GGYRCC 1 cut(s) 1304
BarI GAAGNNNNNNTAC 2 cut(s) 488, 520
BbvCI CCTCAGC 1 cut(s) 465
BbvI GCAGC 1 cut(s) 1087
BccI CCATC 3 cut(s) 316, 410, 686
BciT130I CCWGG 1 cut(s) 927
BclI TGATCA 2 cut(s) 526, 567
BcnI CCSGG 1 cut(s) 125
BcoDI GTCTC 1 cut(s) 1369
BfaI CTAG 1 cut(s) 606
BfmI CTRYAG 1 cut(s) 969
BglII AGATCT 1 cut(s) 1261
BisI GCNGC 1 cut(s) 1101
BlpI GCTNAGC 1 cut(s) 767
BlsI GCNGC 1 cut(s) 1102
Bme1390I CCNGG 2 cut(s) 125, 927
Bme18I GGWCC 1 cut(s) 1428
BmeT110I CYCGRG 2 cut(s) 911, 1368
BmgT120I GGNCC 1 cut(s) 1428
BmiI GGNNCC 2 cut(s) 1306, 1429
BmrFI CCNGG 2 cut(s) 125, 927
BmrI ACTGGG 1 cut(s) 955
BmsI GCATC 2 cut(s) 192, 1321
BmuI ACTGGG 1 cut(s) 955
Bpu10I CCTNAGC 1 cut(s) 465
Bpu1102I GCTNAGC 1 cut(s) 767
Bpu14I TTCGAA 1 cut(s) 40
BpuEI CTTGAG 1 cut(s) 56
BpuMI CCSGG 1 cut(s) 125
BsaAI YACGTR 1 cut(s) 306
BsaJI CCNNGG 4 cut(s) 93, 885, 925, 958
Bsc4I CCNNNNNNNGG 4 cut(s) 233, 234, 801, 1383
Bse1I ACTGG 6 cut(s) 65, 291, 950, 984, 1149, 1308
Bse3DI GCAATG 2 cut(s) 570, 1032
BseBI CCWGG 1 cut(s) 927
BseDI CCNNGG 4 cut(s) 93, 885, 925, 958
BseGI GGATG 2 cut(s) 673, 1333
BseLI CCNNNNNNNGG 4 cut(s) 233, 234, 801, 1383
BseMI GCAATG 2 cut(s) 570, 1032
BseMII CTCAG 4 cut(s) 479, 696, 758, 1428
BseNI ACTGG 6 cut(s) 65, 291, 950, 984, 1149, 1308
BseRI GAGGAG 2 cut(s) 713, 1454
BseXI GCAGC 1 cut(s) 1087
Bsh1236I CGCG 2 cut(s) 14, 51
Bsh1285I CGRYCG 1 cut(s) 124
BshFI GGCC 1 cut(s) 98
BshNI GGYRCC 1 cut(s) 1304
BsiEI CGRYCG 1 cut(s) 124
BsiHKCI CYCGRG 2 cut(s) 911, 1368
BsiSI CCGG 2 cut(s) 124, 508
BslFI GGGAC 1 cut(s) 1441
BslI CCNNNNNNNGG 4 cut(s) 233, 234, 801, 1383
BsmAI GTCTC 1 cut(s) 1369
BsmFI GGGAC 1 cut(s) 1441
BsnI GGCC 1 cut(s) 98
BsoBI CYCGRG 2 cut(s) 911, 1368
Bsp119I TTCGAA 1 cut(s) 40
Bsp143I GATC 4 cut(s) 526, 567, 655, 1261
Bsp1720I GCTNAGC 1 cut(s) 767
Bsp19I CCATGG 1 cut(s) 885
BspACI CCGC 5 cut(s) 12, 128, 704, 1037, 1152
BspANI GGCC 1 cut(s) 98
BspCNI CTCAG 4 cut(s) 478, 695, 759, 1429
BspFNI CGCG 2 cut(s) 14, 51
BspLI GGNNCC 2 cut(s) 1306, 1429
BspPI GGATC 1 cut(s) 650
BspT104I TTCGAA 1 cut(s) 40
BspT107I GGYRCC 1 cut(s) 1304
BsrDI GCAATG 2 cut(s) 570, 1032
BsrI ACTGG 6 cut(s) 65, 291, 950, 984, 1149, 1308
BssECI CCNNGG 4 cut(s) 93, 885, 925, 958
BssMI GATC 4 cut(s) 526, 567, 655, 1261
BssT1I CCWWGG 3 cut(s) 93, 885, 958
Bst2UI CCWGG 1 cut(s) 927
Bst4CI ACNGT 5 cut(s) 210, 1051, 1064, 1125, 1386
Bst6I CTCTTC 2 cut(s) 1170, 1290
BstBAI YACGTR 1 cut(s) 306
BstBI TTCGAA 1 cut(s) 40
BstC8I GCNNGC 2 cut(s) 764, 825
BstDEI CTNAG 7 cut(s) 420, 465, 682, 749, 767, 1068, 1437
BstDSI CCRYGG 1 cut(s) 885
BstENI CCTNNNNNAGG 1 cut(s) 799
BstF5I GGATG 2 cut(s) 673, 1333
BstFNI CGCG 2 cut(s) 14, 51
BstHHI GCGC 1 cut(s) 51
BstKTI GATC 4 cut(s) 529, 570, 658, 1264
BstMAI GTCTC 1 cut(s) 1369
BstMBI GATC 4 cut(s) 526, 567, 655, 1261
BstMCI CGRYCG 1 cut(s) 124
BstMWI GCNNNNNNNGC 4 cut(s) 759, 768, 1006, 1473
BstNI CCWGG 1 cut(s) 927
BstSCI CCNGG 2 cut(s) 123, 925
BstSFI CTRYAG 1 cut(s) 969
BstUI CGCG 2 cut(s) 14, 51
BstV1I GCAGC 1 cut(s) 1087
BstX2I RGATCY 2 cut(s) 655, 1261
BstXI CCANNNNNNTGG 1 cut(s) 945
BstYI RGATCY 2 cut(s) 655, 1261
BsuRI GGCC 1 cut(s) 98
BtgI CCRYGG 1 cut(s) 885
BtsCI GGATG 2 cut(s) 673, 1333
BtsIMutI CAGTG 2 cut(s) 1047, 1434
Cac8I GCNNGC 2 cut(s) 764, 825
CfoI GCGC 1 cut(s) 51
Cfr13I GGNCC 1 cut(s) 1428
CseI GACGC 1 cut(s) 3
Csp6I GTAC 7 cut(s) 160, 230, 284, 307, 496, 831, 1161
CspCI CAANNNNNGTGG 2 cut(s) 1036, 1071
CviAII CATG 6 cut(s) 34, 88, 550, 565, 886, 1129
CviQI GTAC 7 cut(s) 160, 230, 284, 307, 496, 831, 1161
DdeI CTNAG 7 cut(s) 420, 465, 682, 749, 767, 1068, 1437
DpnI GATC 4 cut(s) 528, 569, 657, 1263
DpnII GATC 4 cut(s) 526, 567, 655, 1261
DraI TTTAAA 1 cut(s) 138
DrdI GACNNNNNNGTC 1 cut(s) 1025
DseDI GACNNNNNNGTC 1 cut(s) 1025
EaeI YGGCCR 1 cut(s) 96
Eam1104I CTCTTC 2 cut(s) 1170, 1290
EarI CTCTTC 2 cut(s) 1170, 1290
EciI GGCGGA 2 cut(s) 143, 719
Eco130I CCWWGG 3 cut(s) 93, 885, 958
Eco47I GGWCC 1 cut(s) 1428
Eco57I CTGAAG 3 cut(s) 299, 461, 1063
Eco88I CYCGRG 2 cut(s) 911, 1368
EcoNI CCTNNNNNAGG 1 cut(s) 799
EcoRI GAATTC 1 cut(s) 726
EcoRII CCWGG 1 cut(s) 925
EcoT14I CCWWGG 3 cut(s) 93, 885, 958
ErhI CCWWGG 3 cut(s) 93, 885, 958
FaeI CATG 6 cut(s) 37, 91, 553, 568, 889, 1132
FalI AAGNNNNNCTT 4 cut(s) 434, 466, 1061, 1093
FaqI GGGAC 1 cut(s) 1441
FatI CATG 6 cut(s) 33, 87, 549, 564, 885, 1128
FbaI TGATCA 2 cut(s) 526, 567
Fnu4HI GCNGC 1 cut(s) 1101
FokI GGATG 2 cut(s) 680, 1340
Fsp4HI GCNGC 1 cut(s) 1101
FspBI CTAG 1 cut(s) 606
GlaI GCGC 1 cut(s) 50
GluI GCNGC 1 cut(s) 1101
HaeIII GGCC 1 cut(s) 98
HapII CCGG 2 cut(s) 124, 508
HgaI GACGC 1 cut(s) 3
HhaI GCGC 1 cut(s) 51
Hin1II CATG 6 cut(s) 37, 91, 553, 568, 889, 1132
Hin6I GCGC 1 cut(s) 49
HinP1I GCGC 1 cut(s) 49
HindIII AAGCTT 2 cut(s) 434, 760
HpaII CCGG 2 cut(s) 124, 508
HphI GGTGA 2 cut(s) 331, 1222
Hpy166II GTNNAC 4 cut(s) 162, 232, 562, 817
Hpy188I TCNGA 5 cut(s) 121, 441, 531, 685, 1110
Hpy188III TCNNGA 8 cut(s) 340, 479, 627, 734, 911, 1265, 1370, 1485
Hpy8I GTNNAC 4 cut(s) 162, 232, 562, 817
HpyAV CCTTC 4 cut(s) 485, 624, 658, 1149
HpyCH4III ACNGT 5 cut(s) 210, 1051, 1064, 1125, 1386
HpyCH4IV ACGT 3 cut(s) 305, 577, 1408
HpyCH4V TGCA 8 cut(s) 147, 205, 347, 371, 455, 575, 1103, 1334
HpyF10VI GCNNNNNNNGC 4 cut(s) 759, 768, 1006, 1473
HpyF3I CTNAG 7 cut(s) 420, 465, 682, 749, 767, 1068, 1437
HpySE526I ACGT 3 cut(s) 305, 577, 1408
Hsp92II CATG 6 cut(s) 37, 91, 553, 568, 889, 1132
HspAI GCGC 1 cut(s) 49
Ksp22I TGATCA 2 cut(s) 526, 567
Kzo9I GATC 4 cut(s) 526, 567, 655, 1261
LmnI GCTCC 2 cut(s) 250, 1441
Lsp1109I GCAGC 1 cut(s) 1087
LweI GCATC 2 cut(s) 192, 1321
MaeI CTAG 1 cut(s) 606
MaeII ACGT 3 cut(s) 305, 577, 1408
MaeIII GTNAC 1 cut(s) 1058
MalI GATC 4 cut(s) 528, 569, 657, 1263
MboI GATC 4 cut(s) 526, 567, 655, 1261
MflI RGATCY 2 cut(s) 655, 1261
MlsI TGGCCA 1 cut(s) 98
MluNI TGGCCA 1 cut(s) 98
MlyI GAGTC 1 cut(s) 62
MmeI TCCRAC 5 cut(s) 144, 499, 955, 1326, 1360
Mox20I TGGCCA 1 cut(s) 98
MroXI GAANNNNTTC 3 cut(s) 446, 726, 869
MscI TGGCCA 1 cut(s) 98
MseI TTAA 5 cut(s) 137, 756, 932, 1232, 1290
MslI CAYNNNNRTG 1 cut(s) 1176
Msp20I TGGCCA 1 cut(s) 98
MspI CCGG 2 cut(s) 124, 508
MspR9I CCNGG 2 cut(s) 125, 927
MvaI CCWGG 1 cut(s) 927
MvnI CGCG 2 cut(s) 14, 51
MwoI GCNNNNNNNGC 4 cut(s) 759, 768, 1006, 1473
NciI CCSGG 1 cut(s) 125
NcoI CCATGG 1 cut(s) 885
NdeII GATC 4 cut(s) 526, 567, 655, 1261
NlaIII CATG 6 cut(s) 37, 91, 553, 568, 889, 1132
NlaIV GGNNCC 2 cut(s) 1306, 1429
NspV TTCGAA 1 cut(s) 40
OliI CACNNNNGTG 1 cut(s) 1176
PdmI GAANNNNTTC 3 cut(s) 446, 726, 869
PfeI GAWTC 9 cut(s) 37, 61, 166, 238, 259, 416, 460, 1189, 1373
PflFI GACNNNGTC 1 cut(s) 947
PkrI GCNGC 1 cut(s) 1102
PleI GAGTC 1 cut(s) 61
PpsI GAGTC 1 cut(s) 61
Ppu21I YACGTR 1 cut(s) 306
PshBI ATTAAT 1 cut(s) 1232
Psp6I CCWGG 1 cut(s) 925
PspGI CCWGG 1 cut(s) 925
PspN4I GGNNCC 2 cut(s) 1306, 1429
PspPI GGNCC 1 cut(s) 1428
PsuI RGATCY 2 cut(s) 655, 1261
PsyI GACNNNGTC 1 cut(s) 947
RsaI GTAC 7 cut(s) 161, 231, 285, 308, 497, 832, 1162
RsaNI GTAC 7 cut(s) 160, 230, 284, 307, 496, 831, 1161
RseI CAYNNNNRTG 1 cut(s) 1176
SaqAI TTAA 5 cut(s) 137, 756, 932, 1232, 1290
SatI GCNGC 1 cut(s) 1101
Sau3AI GATC 4 cut(s) 526, 567, 655, 1261
Sau96I GGNCC 1 cut(s) 1428
SchI GAGTC 1 cut(s) 62
ScrFI CCNGG 2 cut(s) 125, 927
SfaNI GCATC 2 cut(s) 192, 1321
SfcI CTRYAG 1 cut(s) 969
SfuI TTCGAA 1 cut(s) 40
SinI GGWCC 1 cut(s) 1428
SmiMI CAYNNNNRTG 1 cut(s) 1176
SmlI CTYRAG 1 cut(s) 71
SmoI CTYRAG 1 cut(s) 71
SsiI CCGC 5 cut(s) 12, 128, 704, 1037, 1152
SspI AATATT 2 cut(s) 858, 880
SspMI CTAG 1 cut(s) 606
StyD4I CCNGG 2 cut(s) 123, 925
StyI CCWWGG 3 cut(s) 93, 885, 958
TaaI ACNGT 5 cut(s) 210, 1051, 1064, 1125, 1386
TaiI ACGT 3 cut(s) 308, 580, 1411
TaqI TCGA 2 cut(s) 40, 192
TatI WGTACW 3 cut(s) 159, 283, 495
TfiI GAWTC 9 cut(s) 37, 61, 166, 238, 259, 416, 460, 1189, 1373
Tru1I TTAA 5 cut(s) 137, 756, 932, 1232, 1290
Tru9I TTAA 5 cut(s) 137, 756, 932, 1232, 1290
TscAI CASTG 2 cut(s) 1054, 1441
TseI GCWGC 1 cut(s) 1100
TspDTI ATGAA 5 cut(s) 369, 429, 538, 687, 1431
TspGWI ACGGA 2 cut(s) 46, 1366
TspRI CASTG 2 cut(s) 1054, 1441
Tth111I GACNNNGTC 1 cut(s) 947
VpaK11BI GGWCC 1 cut(s) 1428
VspI ATTAAT 1 cut(s) 1232
XagI CCTNNNNNAGG 1 cut(s) 799
XapI RAATTY 2 cut(s) 295, 726
XcmI CCANNNNNNNNNTGG 1 cut(s) 892
XmnI GAANNNNTTC 3 cut(s) 446, 726, 869
XspI CTAG 1 cut(s) 606
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.