Rw2G029960

Pre-mRNA-splicing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000718
Physical Location & Seq
Forward (+)
48695439 .. 48704822
9384 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G029960.1

Sequence Viewer

Length: 708 bp
ATGTCTGATGAAGGAAGAGTGCACCCGGATTGCAGGAATGCATCCAATCCCTACCATGAATGCAGTGAGTTTTGCTTCAAAGTTATTGCTGAAACCAAAGCTCGGATGGATCAAAATCAATCAGAATCTGTACAAGTTAGTAGTGGTAGTGGCAAATCAAAATTAGAGGCTACTCAGCAAGAGGAAGATCTAGATAGACCATCAGAGACTGATGAACATGCTAGTGATGGTGATGAGCCTCATGTTGACGAGGAGGAGAATATGGAAGGAGACTTCACGAAGTTCACAGGGAGGAAGAAAAAGTTGTGGGAATTAAGGATGAAGATGGAAAAAAGAATTGGTAAACTTCTGGATGCAAATGGTTTGGATATAAAAAGCGCTTACATGCTAGATACACAAGAGATGGCAGTGTCAAAGTACAAGAAATGGGAAAAGGATCCTGCCCCATATGGCTGGGATGAACAAGGGATAGAGAGAGAGGAAGAAGGGAGAAAAGAAGAGGAGGGGGACGGGAAGCTCTCAGGGAAATCATCAGCCTGGGCAGTCCAACCCGAAGTTGTTGGTGGCCGTCCAGCAGGTATGGAGTTTTCTGCAGAGATTCATGACTTTGTAGTTGAGGATAAAGCCAAGTTCTACCCTTCTGTTAAAGATGATGTATCCATTACTATAATAGAAGCAAGCTCTCATACTTTGGACATGCGAGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
SYF2

Protein Analysis

235

Amino Acids

26.58

Weight (kDa)

4.75

Isoelectric Point (pI)

47.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 566
AclWI GGATC 3 cut(s) 117, 431, 444
AcoI YGGCCR 1 cut(s) 565
AfaI GTAC 2 cut(s) 132, 419
AfeI AGCGCT 1 cut(s) 379
AfiI CCNNNNNNNGG 1 cut(s) 102
AgsI TTSAA 1 cut(s) 79
AjnI CCWGG 1 cut(s) 536
AjuI GAANNNNNNNTTGG 2 cut(s) 321, 353
AluBI AGCT 3 cut(s) 101, 517, 681
AluI AGCT 3 cut(s) 101, 517, 681
Alw21I GWGCWC 1 cut(s) 24
Alw26I GTCTC 2 cut(s) 200, 264
Alw44I GTGCAC 1 cut(s) 20
AlwI GGATC 3 cut(s) 117, 431, 444
AlwNI CAGNNNCTG 2 cut(s) 128, 209
Aor51HI AGCGCT 1 cut(s) 379
AoxI GGCC 1 cut(s) 565
ApaLI GTGCAC 1 cut(s) 20
AspLEI GCGC 1 cut(s) 380
AsuC2I CCSGG 1 cut(s) 26
AsuHPI GGTGA 1 cut(s) 242
BaeGI GKGCMC 1 cut(s) 24
BamHI GGATCC 1 cut(s) 436
Bbv12I GWGCWC 1 cut(s) 24
BccI CCATC 5 cut(s) 100, 208, 221, 319, 397
BceAI ACGGC 1 cut(s) 552
BciT130I CCWGG 1 cut(s) 538
BciVI GTATCC 1 cut(s) 667
BcnI CCSGG 1 cut(s) 26
BcoDI GTCTC 2 cut(s) 200, 264
BfaI CTAG 3 cut(s) 191, 222, 389
BfmI CTRYAG 1 cut(s) 591
BfoI RGCGCY 1 cut(s) 381
BfuAI ACCTGC 1 cut(s) 566
BfuI GTATCC 1 cut(s) 667
BglII AGATCT 1 cut(s) 187
Bme1390I CCNGG 2 cut(s) 26, 538
BmiI GGNNCC 1 cut(s) 438
BmrFI CCNGG 2 cut(s) 26, 538
BmsI GCATC 2 cut(s) 50, 343
BpuMI CCSGG 1 cut(s) 26
BsaBI GATNNNNATC 1 cut(s) 114
BsaJI CCNNGG 1 cut(s) 537
Bsc4I CCNNNNNNNGG 1 cut(s) 102
Bse8I GATNNNNATC 1 cut(s) 114
BseBI CCWGG 1 cut(s) 538
BseDI CCNNGG 1 cut(s) 537
BseGI GGATG 5 cut(s) 41, 111, 324, 358, 463
BseJI GATNNNNATC 1 cut(s) 114
BseLI CCNNNNNNNGG 1 cut(s) 102
BseMII CTCAG 2 cut(s) 188, 534
BseRI GAGGAG 3 cut(s) 266, 269, 515
BseSI GKGCMC 1 cut(s) 24
BseYI CCCAGC 1 cut(s) 453
BshFI GGCC 1 cut(s) 567
BsiHKAI GWGCWC 1 cut(s) 24
BsiSI CCGG 1 cut(s) 26
BslFI GGGAC 1 cut(s) 521
BslI CCNNNNNNNGG 1 cut(s) 102
BsmAI GTCTC 2 cut(s) 200, 264
BsmFI GGGAC 1 cut(s) 521
BsmI GAATGC 2 cut(s) 43, 65
BsnI GGCC 1 cut(s) 567
Bsp1286I GDGCHC 1 cut(s) 24
Bsp1407I TGTACA 1 cut(s) 130
Bsp143I GATC 3 cut(s) 109, 187, 436
BspANI GGCC 1 cut(s) 567
BspCNI CTCAG 2 cut(s) 187, 533
BspHI TCATGA 1 cut(s) 601
BspLI GGNNCC 1 cut(s) 438
BspMAI CTGCAG 1 cut(s) 595
BspMI ACCTGC 1 cut(s) 566
BspPI GGATC 3 cut(s) 117, 431, 444
BsrGI TGTACA 1 cut(s) 130
BssECI CCNNGG 1 cut(s) 537
BssMI GATC 3 cut(s) 109, 187, 436
Bst2UI CCWGG 1 cut(s) 538
Bst6I CTCTTC 2 cut(s) 10, 492
BstAUI TGTACA 1 cut(s) 130
BstC8I GCNNGC 1 cut(s) 679
BstDEI CTNAG 2 cut(s) 174, 520
BstF5I GGATG 5 cut(s) 41, 111, 324, 358, 463
BstH2I RGCGCY 1 cut(s) 381
BstHHI GCGC 1 cut(s) 380
BstKTI GATC 3 cut(s) 112, 190, 439
BstMAI GTCTC 2 cut(s) 200, 264
BstMBI GATC 3 cut(s) 109, 187, 436
BstNI CCWGG 1 cut(s) 538
BstNSI RCATGY 3 cut(s) 221, 388, 700
BstSCI CCNGG 2 cut(s) 24, 536
BstSFI CTRYAG 1 cut(s) 591
BstSLI GKGCMC 1 cut(s) 24
BstX2I RGATCY 2 cut(s) 187, 436
BstXI CCANNNNNNTGG 1 cut(s) 453
BstYI RGATCY 2 cut(s) 187, 436
BsuI GTATCC 1 cut(s) 667
BsuRI GGCC 1 cut(s) 567
BtsCI GGATG 5 cut(s) 41, 111, 324, 358, 463
BtsI GCAGTG 2 cut(s) 70, 414
BtsIMutI CAGTG 2 cut(s) 70, 414
BveI ACCTGC 1 cut(s) 566
Cac8I GCNNGC 1 cut(s) 679
CaiI CAGNNNCTG 2 cut(s) 128, 209
CciI TCATGA 1 cut(s) 601
CfoI GCGC 1 cut(s) 380
Csp6I GTAC 2 cut(s) 131, 418
CviAII CATG 6 cut(s) 56, 218, 242, 385, 602, 697
CviJI RGCY 9 cut(s) 101, 170, 238, 453, 517, 536, 567, 626, 681
CviKI_1 RGCY 9 cut(s) 101, 170, 238, 453, 517, 536, 567, 626, 681
CviQI GTAC 2 cut(s) 131, 418
DdeI CTNAG 2 cut(s) 174, 520
DpnI GATC 3 cut(s) 111, 189, 438
DpnII GATC 3 cut(s) 109, 187, 436
EaeI YGGCCR 1 cut(s) 565
Eam1104I CTCTTC 2 cut(s) 10, 492
EarI CTCTTC 2 cut(s) 10, 492
Eco47III AGCGCT 1 cut(s) 379
EcoRII CCWGG 1 cut(s) 536
EcoT22I ATGCAT 1 cut(s) 43
FaeI CATG 6 cut(s) 59, 221, 245, 388, 605, 700
FaqI GGGAC 1 cut(s) 521
FatI CATG 6 cut(s) 55, 217, 241, 384, 601, 696
FauNDI CATATG 1 cut(s) 448
FokI GGATG 5 cut(s) 28, 118, 331, 365, 470
FspBI CTAG 3 cut(s) 191, 222, 389
GlaI GCGC 1 cut(s) 379
GsaI CCCAGC 1 cut(s) 457
HaeII RGCGCY 1 cut(s) 381
HaeIII GGCC 1 cut(s) 567
HapII CCGG 1 cut(s) 26
HhaI GCGC 1 cut(s) 380
Hin1II CATG 6 cut(s) 59, 221, 245, 388, 605, 700
Hin6I GCGC 1 cut(s) 378
HinP1I GCGC 1 cut(s) 378
HincII GTYRAC 1 cut(s) 247
HindII GTYRAC 1 cut(s) 247
HinfI GANTC 2 cut(s) 125, 598
HpaII CCGG 1 cut(s) 26
HphI GGTGA 1 cut(s) 242
Hpy166II GTNNAC 4 cut(s) 22, 247, 285, 344
Hpy188I TCNGA 4 cut(s) 7, 105, 124, 205
Hpy188III TCNNGA 4 cut(s) 191, 277, 350, 602
Hpy8I GTNNAC 4 cut(s) 22, 247, 285, 344
HpyAV CCTTC 4 cut(s) 5, 260, 479, 648
HpyCH4V TGCA 6 cut(s) 22, 33, 41, 63, 356, 593
HpyF3I CTNAG 2 cut(s) 174, 520
Hsp92II CATG 6 cut(s) 59, 221, 245, 388, 605, 700
HspAI GCGC 1 cut(s) 378
Kzo9I GATC 3 cut(s) 109, 187, 436
LweI GCATC 2 cut(s) 50, 343
MaeI CTAG 3 cut(s) 191, 222, 389
MalI GATC 3 cut(s) 111, 189, 438
MboI GATC 3 cut(s) 109, 187, 436
MboII GAAGA 6 cut(s) 27, 197, 307, 334, 494, 509
MflI RGATCY 2 cut(s) 187, 436
MhlI GDGCHC 1 cut(s) 24
MluCI AATT 3 cut(s) 161, 311, 336
MmeI TCCRAC 1 cut(s) 571
Mph1103I ATGCAT 1 cut(s) 43
MseI TTAA 2 cut(s) 314, 645
MslI CAYNNNNRTG 2 cut(s) 222, 701
MspI CCGG 1 cut(s) 26
MspR9I CCNGG 2 cut(s) 26, 538
Mva1269I GAATGC 2 cut(s) 43, 65
MvaI CCWGG 1 cut(s) 538
NciI CCSGG 1 cut(s) 26
NdeI CATATG 1 cut(s) 448
NdeII GATC 3 cut(s) 109, 187, 436
NlaIII CATG 6 cut(s) 59, 221, 245, 388, 605, 700
NlaIV GGNNCC 1 cut(s) 438
NsiI ATGCAT 1 cut(s) 43
NspI RCATGY 3 cut(s) 221, 388, 700
PagI TCATGA 1 cut(s) 601
PctI GAATGC 2 cut(s) 43, 65
PfeI GAWTC 2 cut(s) 125, 598
Psp6I CCWGG 1 cut(s) 536
PspFI CCCAGC 1 cut(s) 453
PspGI CCWGG 1 cut(s) 536
PspN4I GGNNCC 1 cut(s) 438
PstI CTGCAG 1 cut(s) 595
PstNI CAGNNNCTG 2 cut(s) 128, 209
PsuI RGATCY 2 cut(s) 187, 436
RsaI GTAC 2 cut(s) 132, 419
RsaNI GTAC 2 cut(s) 131, 418
RseI CAYNNNNRTG 2 cut(s) 222, 701
SaqAI TTAA 2 cut(s) 314, 645
Sau3AI GATC 3 cut(s) 109, 187, 436
ScrFI CCNGG 2 cut(s) 26, 538
SduI GDGCHC 1 cut(s) 24
SetI ASST 4 cut(s) 103, 519, 580, 683
SfaNI GCATC 2 cut(s) 50, 343
SfcI CTRYAG 1 cut(s) 591
SmiMI CAYNNNNRTG 2 cut(s) 222, 701
Sse9I AATT 3 cut(s) 161, 311, 336
SspMI CTAG 3 cut(s) 191, 222, 389
StyD4I CCNGG 2 cut(s) 24, 536
TasI AATT 3 cut(s) 161, 311, 336
TatI WGTACW 2 cut(s) 130, 417
TfiI GAWTC 2 cut(s) 125, 598
Tru1I TTAA 2 cut(s) 314, 645
Tru9I TTAA 2 cut(s) 314, 645
TscAI CASTG 2 cut(s) 70, 414
TspDTI ATGAA 6 cut(s) 24, 72, 228, 335, 474, 590
TspRI CASTG 2 cut(s) 70, 414
VneI GTGCAC 1 cut(s) 20
XbaI TCTAGA 1 cut(s) 190
XceI RCATGY 3 cut(s) 221, 388, 700
XcmI CCANNNNNNNNNTGG 1 cut(s) 103
XspI CTAG 3 cut(s) 191, 222, 389
Zsp2I ATGCAT 1 cut(s) 43
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.