FvH4_6g54352

Protein of unknown function (DUF3754)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000718
Physical Location & Seq
Forward (+)
39712776 .. 39716954
4179 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g54352.t1

Sequence Viewer

Length: 1473 bp
ATGGTACAAAAGAAAGAGATTATTCGATTAGAGCGTGAATCCGTCATTGGAGTTATCAAGCCCAGACTCATCATGACCTTGGCCAATCTCATCGAGCAAAGTTCTGACCGGACAGAGTTTTTGAAGCTTTGCAAGAGGGTTGAGTACACAATTAGGGCTTGGTATCTTCTACAATTTGAGGATTTGATGCAACTCTACTCCCTATTTGATCCTGTTCATGGAGCCCGGATGTTGGAACAACAGAAACTTGATGATAAAGAAATTGATGTGCTTGAACAAAATTTCCTCACCTACTTGTTTCAGGTGATGGAAAAGAGTAATTTTAAGATTGCAGATAATGAAGAAATCGAGGTTGCAACTTCAGGGCAGTATCTTCTAAATCTTCCCATCGTGGTTGATGAGTCTAAGCTTGACAACAAACTTTTGAAGAAGTATTTTGAAGATCATCCTACCGAAAACATTCCAGATTTTTCTGATAAGTATGTCATCTTCCGACGCGGCATTGGACTTGACAAGACAACCAATTGGTTTTTCATGGAGAAGATGGACATCATTATTTCACGTTTCTGGAAATATCTTTTACGAATAACTAAGATAGAAAAAATTTTCATGAAACCAACCTTTAGGAAGAGGGACTCAGAATCTGATACAGATGAGGATGATTTCTTTGTTGAACGAATCCGTCTTGAAAACATGGAACTAACCCCTCAAACTTTGCTTCGTATGAACACAATCCAGGAGCCAACTTTTGATAGGATTATTGTGGTTTACAGGCAAGCGACTACCGTCGAGGATCGTCCAGAACGGGGAATACATATCAAACATTTCAAAAGTATACCCATGGCTGATTTGGAGATAGTTCTTCCTGAAAAGAAGAACCCAGGTTTAAAACCAATGGACTGGCTCTCCTTCATTGCTTCTGCTGTAGTTGGTCTGGTTGCTGTTGTTACTTCGATTGAAACTTCTAAAGTCGATCTTTGGGTTATTTTTGCAATTATTTCCACTGTGATTGGTTACTGTGCTAAGACATATTTCAGTTTTCAGCAGAACTTAGCTCAATATCAGAACTTGATAACACAGTCCATGTACGAAAAACAACTAGATAGCGGAAGAGGAACCCTTCTTCACTTGTGTGATGACGTGATTCAACGGGAAGTCAAGGAAGTAATAGTTTGTTTCTATATGTTAATGGAACAAGGGAAAGCTACTTTAACAGATCTGGACAGGTGGTGTGAGGACCTCATTAAAGAACAGTTCAACGACGATATCAATTTTGATGTGGACGATGCAGTTAAGAAGTTGGAGAAATTGGGAATTGTTACTCGGGATGCCCTTGGACGGTATAGCATTGTGGGGCTGAAACGTGCTAACGAGGTTATTGGCATCACCACTGAGGAGGTTGTTCTCAAGGCAAAACAAGATGCTGCTTATGCTCCTGCTTCTCCTGCAGGCCAGGGCGCTGACTATAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3754

Protein Analysis

491

Amino Acids

57.09

Weight (kDa)

5.19

Isoelectric Point (pI)

31.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3754 PF12576 250 - 372 1.1e-23 Protein of unknown function (DUF3754)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 835
AccII CGCG 1 cut(s) 498
AciI CCGC 2 cut(s) 498, 1107
AclWI GGATC 2 cut(s) 203, 801
AcoI YGGCCR 1 cut(s) 81
AcsI RAATTY 2 cut(s) 280, 603
AcuI CTGAAG 1 cut(s) 345
AfaI GTAC 3 cut(s) 6, 146, 1088
AfiI CCNNNNNNNGG 4 cut(s) 218, 232, 806, 1338
AjiI CACGTC 1 cut(s) 1141
AjnI CCWGG 3 cut(s) 735, 880, 1452
AjuI GAANNNNNNNTTGG 4 cut(s) 30, 62, 515, 547
AleI CACNNNNGTG 1 cut(s) 1131
AloI GAACNNNNNNTCC 2 cut(s) 267, 299
AluBI AGCT 4 cut(s) 127, 409, 1055, 1205
AluI AGCT 4 cut(s) 127, 409, 1055, 1205
AlwI GGATC 2 cut(s) 203, 801
AlwNI CAGNNNCTG 1 cut(s) 644
Ama87I CYCGRG 1 cut(s) 1323
AoxI GGCC 2 cut(s) 81, 1450
ApeKI GCWGC 1 cut(s) 1424
ApoI RAATTY 2 cut(s) 280, 603
ArsI GACNNNNNNTTYG 2 cut(s) 103, 135
Asp700I GAANNNNTTC 1 cut(s) 459
AspLEI GCGC 1 cut(s) 1460
AspS9I GGNCC 1 cut(s) 1237
AsuC2I CCSGG 1 cut(s) 226
AsuHPI GGTGA 3 cut(s) 280, 316, 1378
AvaI CYCGRG 1 cut(s) 1323
AvaII GGWCC 1 cut(s) 1237
BalI TGGCCA 1 cut(s) 83
BanII GRGCYC 1 cut(s) 226
BarI GAAGNNNNNNTAC 2 cut(s) 473, 505
BbvI GCAGC 1 cut(s) 1411
BccI CCATC 3 cut(s) 301, 395, 538
BciT130I CCWGG 3 cut(s) 737, 882, 1454
BcnI CCSGG 1 cut(s) 226
BfaI CTAG 1 cut(s) 1100
BfmI CTRYAG 3 cut(s) 924, 1446, 1465
BfoI RGCGCY 1 cut(s) 1461
BglII AGATCT 1 cut(s) 1216
BisI GCNGC 2 cut(s) 499, 1425
BlsI GCNGC 2 cut(s) 500, 1426
Bme1390I CCNGG 4 cut(s) 226, 737, 882, 1454
Bme18I GGWCC 1 cut(s) 1237
BmeT110I CYCGRG 1 cut(s) 1323
BmgBI CACGTC 1 cut(s) 1141
BmgT120I GGNCC 1 cut(s) 1237
BmiI GGNNCC 3 cut(s) 223, 741, 1117
BmrFI CCNGG 4 cut(s) 226, 737, 882, 1454
BmsI GCATC 5 cut(s) 177, 1276, 1318, 1392, 1411
BoxI GACNNNNGTC 1 cut(s) 785
BpuEI CTTGAG 1 cut(s) 1391
BpuMI CCSGG 1 cut(s) 226
BsaBI GATNNNNATC 1 cut(s) 548
BsaJI CCNNGG 5 cut(s) 78, 840, 880, 1333, 1453
BsaWI WCCGGW 1 cut(s) 108
BsaXI ACNNNNNCTCC 2 cut(s) 890, 920
Bsc4I CCNNNNNNNGG 4 cut(s) 218, 232, 806, 1338
Bse1I ACTGG 1 cut(s) 905
Bse3DI GCAATG 1 cut(s) 912
Bse8I GATNNNNATC 1 cut(s) 548
BseBI CCWGG 3 cut(s) 737, 882, 1454
BseDI CCNNGG 5 cut(s) 78, 840, 880, 1333, 1453
BseGI GGATG 4 cut(s) 234, 445, 664, 1333
BseJI GATNNNNATC 1 cut(s) 548
BseLI CCNNNNNNNGG 4 cut(s) 218, 232, 806, 1338
BseMI GCAATG 1 cut(s) 912
BseMII CTCAG 2 cut(s) 651, 1383
BseNI ACTGG 1 cut(s) 905
BseRI GAGGAG 1 cut(s) 1409
BseXI GCAGC 1 cut(s) 1411
Bsh1236I CGCG 1 cut(s) 498
BshFI GGCC 2 cut(s) 83, 1452
BsiHKCI CYCGRG 1 cut(s) 1323
BsiSI CCGG 2 cut(s) 109, 226
BslFI GGGAC 1 cut(s) 647
BslI CCNNNNNNNGG 4 cut(s) 218, 232, 806, 1338
BsmFI GGGAC 1 cut(s) 647
BsnI GGCC 2 cut(s) 83, 1452
BsoBI CYCGRG 1 cut(s) 1323
Bsp1286I GDGCHC 1 cut(s) 226
Bsp143I GATC 5 cut(s) 208, 442, 793, 973, 1216
Bsp19I CCATGG 1 cut(s) 840
BspACI CCGC 2 cut(s) 498, 1107
BspANI GGCC 2 cut(s) 83, 1452
BspCNI CTCAG 2 cut(s) 650, 1384
BspFNI CGCG 1 cut(s) 498
BspHI TCATGA 2 cut(s) 72, 609
BspLI GGNNCC 3 cut(s) 223, 741, 1117
BspMAI CTGCAG 1 cut(s) 1450
BspPI GGATC 2 cut(s) 203, 801
BsrDI GCAATG 1 cut(s) 912
BsrI ACTGG 1 cut(s) 905
BssECI CCNNGG 5 cut(s) 78, 840, 880, 1333, 1453
BssMI GATC 5 cut(s) 208, 442, 793, 973, 1216
BssNAI GTATAC 1 cut(s) 836
BssT1I CCWWGG 3 cut(s) 78, 840, 1333
Bst1107I GTATAC 1 cut(s) 836
Bst2UI CCWGG 3 cut(s) 737, 882, 1454
Bst4CI ACNGT 6 cut(s) 787, 1006, 1019, 1080, 1254, 1341
Bst6I CTCTTC 2 cut(s) 623, 1105
BstC8I GCNNGC 2 cut(s) 777, 1450
BstDEI CTNAG 6 cut(s) 405, 591, 637, 1023, 1051, 1392
BstDSI CCRYGG 1 cut(s) 840
BstF5I GGATG 4 cut(s) 234, 445, 664, 1333
BstFNI CGCG 1 cut(s) 498
BstH2I RGCGCY 1 cut(s) 1461
BstHHI GCGC 1 cut(s) 1460
BstKTI GATC 5 cut(s) 211, 445, 796, 976, 1219
BstMBI GATC 5 cut(s) 208, 442, 793, 973, 1216
BstMWI GCNNNNNNNGC 2 cut(s) 1430, 1445
BstNI CCWGG 3 cut(s) 737, 882, 1454
BstPAI GACNNNNGTC 1 cut(s) 785
BstSCI CCNGG 4 cut(s) 224, 735, 880, 1452
BstSFI CTRYAG 3 cut(s) 924, 1446, 1465
BstUI CGCG 1 cut(s) 498
BstV1I GCAGC 1 cut(s) 1411
BstX2I RGATCY 1 cut(s) 1216
BstXI CCANNNNNNTGG 1 cut(s) 900
BstYI RGATCY 1 cut(s) 1216
BstZ17I GTATAC 1 cut(s) 836
BsuRI GGCC 2 cut(s) 83, 1452
BtgI CCRYGG 1 cut(s) 840
BtrI CACGTC 1 cut(s) 1141
BtsCI GGATG 4 cut(s) 234, 445, 664, 1333
BtsIMutI CAGTG 2 cut(s) 1002, 1389
Cac8I GCNNGC 2 cut(s) 777, 1450
CaiI CAGNNNCTG 1 cut(s) 644
CciI TCATGA 2 cut(s) 72, 609
CfoI GCGC 1 cut(s) 1460
Cfr13I GGNCC 1 cut(s) 1237
CseI GACGC 1 cut(s) 504
Csp6I GTAC 3 cut(s) 5, 145, 1087
CspCI CAANNNNNGTGG 2 cut(s) 991, 1026
CviAII CATG 7 cut(s) 73, 218, 535, 610, 694, 841, 1084
CviQI GTAC 3 cut(s) 5, 145, 1087
DdeI CTNAG 6 cut(s) 405, 591, 637, 1023, 1051, 1392
DpnI GATC 5 cut(s) 210, 444, 795, 975, 1218
DpnII GATC 5 cut(s) 208, 442, 793, 973, 1216
DraI TTTAAA 1 cut(s) 888
EaeI YGGCCR 1 cut(s) 81
Eam1104I CTCTTC 2 cut(s) 623, 1105
EarI CTCTTC 2 cut(s) 623, 1105
Eco130I CCWWGG 3 cut(s) 78, 840, 1333
Eco24I GRGCYC 1 cut(s) 226
Eco32I GATATC 1 cut(s) 1267
Eco47I GGWCC 1 cut(s) 1237
Eco57I CTGAAG 1 cut(s) 345
Eco88I CYCGRG 1 cut(s) 1323
EcoO109I RGGNCCY 1 cut(s) 1237
EcoRII CCWGG 3 cut(s) 735, 880, 1452
EcoRV GATATC 1 cut(s) 1267
EcoT14I CCWWGG 3 cut(s) 78, 840, 1333
EcoT38I GRGCYC 1 cut(s) 226
ErhI CCWWGG 3 cut(s) 78, 840, 1333
FaeI CATG 7 cut(s) 76, 221, 538, 613, 697, 844, 1087
FalI AAGNNNNNCTT 2 cut(s) 960, 992
FaqI GGGAC 1 cut(s) 647
FatI CATG 7 cut(s) 72, 217, 534, 609, 693, 840, 1083
FblI GTMKAC 1 cut(s) 835
Fnu4HI GCNGC 2 cut(s) 499, 1425
FokI GGATG 4 cut(s) 241, 432, 671, 1340
FriOI GRGCYC 1 cut(s) 226
Fsp4HI GCNGC 2 cut(s) 499, 1425
FspBI CTAG 1 cut(s) 1100
GlaI GCGC 1 cut(s) 1459
GluI GCNGC 2 cut(s) 499, 1425
HaeII RGCGCY 1 cut(s) 1461
HaeIII GGCC 2 cut(s) 83, 1452
HapII CCGG 2 cut(s) 109, 226
HgaI GACGC 1 cut(s) 504
HhaI GCGC 1 cut(s) 1460
Hin1II CATG 7 cut(s) 76, 221, 538, 613, 697, 844, 1087
Hin6I GCGC 1 cut(s) 1458
HinP1I GCGC 1 cut(s) 1458
HindIII AAGCTT 2 cut(s) 125, 407
HinfI GANTC 7 cut(s) 38, 66, 401, 635, 641, 678, 1144
HpaII CCGG 2 cut(s) 109, 226
HphI GGTGA 3 cut(s) 280, 316, 1378
Hpy166II GTNNAC 4 cut(s) 147, 769, 836, 1282
Hpy188I TCNGA 6 cut(s) 106, 475, 494, 640, 646, 1065
Hpy188III TCNNGA 9 cut(s) 73, 464, 568, 610, 686, 800, 866, 1220, 1325
Hpy8I GTNNAC 4 cut(s) 147, 769, 836, 1282
Hpy99I CGWCG 3 cut(s) 498, 791, 1265
HpyAV CCTTC 2 cut(s) 919, 1130
HpyCH4III ACNGT 6 cut(s) 787, 1006, 1019, 1080, 1254, 1341
HpyCH4IV ACGT 3 cut(s) 562, 1140, 1363
HpyCH4V TGCA 7 cut(s) 132, 190, 332, 356, 992, 1289, 1448
HpyF10VI GCNNNNNNNGC 2 cut(s) 1430, 1445
HpyF3I CTNAG 6 cut(s) 405, 591, 637, 1023, 1051, 1392
HpySE526I ACGT 3 cut(s) 562, 1140, 1363
Hsp92II CATG 7 cut(s) 76, 221, 538, 613, 697, 844, 1087
HspAI GCGC 1 cut(s) 1458
Kzo9I GATC 5 cut(s) 208, 442, 793, 973, 1216
LmnI GCTCC 3 cut(s) 221, 739, 1438
Lsp1109I GCAGC 1 cut(s) 1411
LweI GCATC 5 cut(s) 177, 1276, 1318, 1392, 1411
MaeI CTAG 1 cut(s) 1100
MaeII ACGT 3 cut(s) 562, 1140, 1363
MaeIII GTNAC 3 cut(s) 946, 1013, 1318
MalI GATC 5 cut(s) 210, 444, 795, 975, 1218
MboI GATC 5 cut(s) 208, 442, 793, 973, 1216
MfeI CAATTG 1 cut(s) 523
MflI RGATCY 1 cut(s) 1216
MhlI GDGCHC 1 cut(s) 226
MlsI TGGCCA 1 cut(s) 83
MluNI TGGCCA 1 cut(s) 83
MlyI GAGTC 3 cut(s) 60, 410, 629
MmeI TCCRAC 3 cut(s) 213, 517, 1281
Mox20I TGGCCA 1 cut(s) 83
MroXI GAANNNNTTC 1 cut(s) 459
MscI TGGCCA 1 cut(s) 83
MseI TTAA 6 cut(s) 324, 887, 1187, 1211, 1245, 1293
MslI CAYNNNNRTG 1 cut(s) 1131
Msp20I TGGCCA 1 cut(s) 83
MspI CCGG 2 cut(s) 109, 226
MspR9I CCNGG 4 cut(s) 226, 737, 882, 1454
MunI CAATTG 1 cut(s) 523
MvaI CCWGG 3 cut(s) 737, 882, 1454
MvnI CGCG 1 cut(s) 498
MwoI GCNNNNNNNGC 2 cut(s) 1430, 1445
NciI CCSGG 1 cut(s) 226
NcoI CCATGG 1 cut(s) 840
NdeII GATC 5 cut(s) 208, 442, 793, 973, 1216
NlaIII CATG 7 cut(s) 76, 221, 538, 613, 697, 844, 1087
NlaIV GGNNCC 3 cut(s) 223, 741, 1117
OliI CACNNNNGTG 1 cut(s) 1131
PagI TCATGA 2 cut(s) 72, 609
PcsI WCGNNNNNNNCGW 1 cut(s) 31
PdmI GAANNNNTTC 1 cut(s) 459
PfeI GAWTC 4 cut(s) 38, 641, 678, 1144
PfoI TCCNGGA 1 cut(s) 735
PkrI GCNGC 2 cut(s) 500, 1426
PleI GAGTC 3 cut(s) 60, 409, 629
PpsI GAGTC 3 cut(s) 60, 409, 629
PpuMI RGGWCCY 1 cut(s) 1237
PshAI GACNNNNGTC 1 cut(s) 785
Psp5II RGGWCCY 1 cut(s) 1237
Psp6I CCWGG 3 cut(s) 735, 880, 1452
PspGI CCWGG 3 cut(s) 735, 880, 1452
PspN4I GGNNCC 3 cut(s) 223, 741, 1117
PspPI GGNCC 1 cut(s) 1237
PspPPI RGGWCCY 1 cut(s) 1237
PsrI GAACNNNNNNTAC 2 cut(s) 795, 827
PstI CTGCAG 1 cut(s) 1450
PstNI CAGNNNCTG 1 cut(s) 644
PsuI RGATCY 1 cut(s) 1216
RsaI GTAC 3 cut(s) 6, 146, 1088
RsaNI GTAC 3 cut(s) 5, 145, 1087
RseI CAYNNNNRTG 1 cut(s) 1131
SaqAI TTAA 6 cut(s) 324, 887, 1187, 1211, 1245, 1293
SatI GCNGC 2 cut(s) 499, 1425
Sau3AI GATC 5 cut(s) 208, 442, 793, 973, 1216
Sau96I GGNCC 1 cut(s) 1237
SbfI CCTGCAGG 1 cut(s) 1450
SchI GAGTC 3 cut(s) 60, 410, 629
ScrFI CCNGG 4 cut(s) 226, 737, 882, 1454
SdaI CCTGCAGG 1 cut(s) 1450
SduI GDGCHC 1 cut(s) 226
SfaNI GCATC 5 cut(s) 177, 1276, 1318, 1392, 1411
SfcI CTRYAG 3 cut(s) 924, 1446, 1465
SinI GGWCC 1 cut(s) 1237
SmiMI CAYNNNNRTG 1 cut(s) 1131
SmlI CTYRAG 1 cut(s) 1406
SmoI CTYRAG 1 cut(s) 1406
Sse8387I CCTGCAGG 1 cut(s) 1450
SsiI CCGC 2 cut(s) 498, 1107
SspMI CTAG 1 cut(s) 1100
StyD4I CCNGG 4 cut(s) 224, 735, 880, 1452
StyI CCWWGG 3 cut(s) 78, 840, 1333
TaaI ACNGT 6 cut(s) 787, 1006, 1019, 1080, 1254, 1341
TaiI ACGT 3 cut(s) 565, 1143, 1366
TaqI TCGA 6 cut(s) 25, 93, 348, 789, 953, 972
TatI WGTACW 1 cut(s) 144
TauI GCSGC 1 cut(s) 501
TfiI GAWTC 4 cut(s) 38, 641, 678, 1144
Tru1I TTAA 6 cut(s) 324, 887, 1187, 1211, 1245, 1293
Tru9I TTAA 6 cut(s) 324, 887, 1187, 1211, 1245, 1293
TscAI CASTG 2 cut(s) 1009, 1396
TseI GCWGC 1 cut(s) 1424
TspDTI ATGAA 7 cut(s) 206, 354, 523, 598, 626, 740, 901
TspGWI ACGGA 2 cut(s) 31, 671
TspRI CASTG 2 cut(s) 1009, 1396
VpaK11BI GGWCC 1 cut(s) 1237
XapI RAATTY 2 cut(s) 280, 603
XcmI CCANNNNNNNNNTGG 1 cut(s) 847
XmiI GTMKAC 1 cut(s) 835
XmnI GAANNNNTTC 1 cut(s) 459
XspI CTAG 1 cut(s) 1100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.