MD09G1002100.v1.1

Protein of unknown function (DUF3754)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000718
Physical Location & Seq
Reverse (-)
248702 .. 251992
3291 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1002100.v1.1.491

Sequence Viewer

Length: 1404 bp
ATGGGTAAGAAAGACATGATTCGGTTAGAGCGTGAATCCGCCATTCAAGTTCTCAAGCCCAAACTCTTCATGACCTTGGCTAACCTCATCGGTATGTACTTTGTACTTGAAAGTTCTGATCGCGCTGAGTTTCTCAAGCTCTGTAAGAGAGTCGAGTACACAATTCGGGCTTGGTATCTTCTTCAATTTGAGGATTTGATGCAACTATACTCCCTGTTTGATCCTGTCCATGGGAGTCAGAAATTGGAGCAGCAGAAGCTTTCTCCTCAAGAAATTGATGTACTTGAACAGAACTTCCTCAAGTACTTGTTTCAGGTGATGGAAAAGAGTAATTTCAAGATTGCGACTAACGAAGAAATTGAGGTTGCGCAGTCGGGGCAGTATCTTCTAAACCTTCCCATCGTTGTTGATGAATCTAAGTACGTCATCTTCCGACGCGGCATTGGACTTGATAAGACTTCAGATTACTTTATCATGGAGAAAGTGGACATGATCATTGCACGTTGCTGGAAATATCTTTTAAGACTAACCAAGACAGAAAAGTTCTTTTCGAAAAAGAAAGAGGCACAGTGCAAGATAGATCCAAAGAAGGATGATAATATTTGCCTCCCTGACCAAGATCCAGAGGACTTGTTTGTTGAACGAATCCGTATTGAAAAAATGGAACTGAGCATTCAAAATTTGCTGTGCAAGAACACAATCCAAGAGCCAACTTTTGATAGGATTATTGTTGTTTATAGGCGAGCAAGTACCGAAGATAATGAAGAGCGGGGAATATATGTTAAACATTTTAAAAACATTCCAATGGCCGATATGGAGATTGTTCTTCCTGAGAAGAAGAACCCAGGATTAACTCCAATGGACTGGCTCACGTTCGTTGGCTCTGCCGTAGTAGGGCTGGTTGCAGTTGTTACTTCGCTCGAAATGCCTCAAATTGATCTTTGGGTCATTTTTGCGATTATTTCCACTGTGATTGGTTACTTTGCTAAGACATACTTCAGTTATCAGCAGAACTTGGCTCAGTATCAGAACTTGATAACACAGTCCATGTATGAAAAACAATTGGATAGCGGACGGGGTACTCTCCTTCACTTGTGCGATGATGTGATTCAACAGGAAGTGAAGGAGGTGATAATTTCATTTTACATATTAATGGAACAAGGGAAAGCTACCTTACTAGATCTGGACAAGCGGTGTGAGGAACTAATCAAAGAACAGTTTAACGACGACTGTAATTTTGATGTGGACGATGCAGTTCAGAAGTTGGAGAAACTGGGAATTGTTACTCGGGATACCCTTGGACGATACAACAGCGTAGGGCTGAAACGTGCGAACGAGATCATTGGCACCACCACAGAGGAGGTTGTTCTTAAGGCAAAACAAGATTCCACCCCCAATGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3754

Protein Analysis

468

Amino Acids

54.26

Weight (kDa)

5.18

Isoelectric Point (pI)

34.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3754 PF12576 239 - 360 3.4e-24 Protein of unknown function (DUF3754)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 369
AccB1I GGYRCC 1 cut(s) 1346
AccBSI CCGCTC 1 cut(s) 769
AccII CGCG 2 cut(s) 123, 438
AciI CCGC 5 cut(s) 39, 438, 769, 1071, 1192
AclWI GGATC 3 cut(s) 215, 575, 614
AcoI YGGCCR 1 cut(s) 807
AcsI RAATTY 1 cut(s) 679
AcuI CTGAAG 2 cut(s) 444, 982
AfaI GTAC 8 cut(s) 98, 105, 158, 282, 305, 422, 751, 1081
AfiI CCNNNNNNNGG 2 cut(s) 230, 894
AflII CTTAAG 1 cut(s) 1370
AgsI TTSAA 9 cut(s) 47, 110, 185, 287, 337, 641, 656, 677, 1112
AjnI CCWGG 1 cut(s) 844
AloI GAACNNNNNNTCC 2 cut(s) 279, 311
AluBI AGCT 3 cut(s) 139, 259, 1169
AluI AGCT 3 cut(s) 139, 259, 1169
AlwI GGATC 3 cut(s) 215, 575, 614
Ama87I CYCGRG 1 cut(s) 1287
AoxI GGCC 1 cut(s) 807
ApeKI GCWGC 1 cut(s) 250
ApoI RAATTY 1 cut(s) 679
ArsI GACNNNNNNTTYG 2 cut(s) 1220, 1252
AseI ATTAAT 1 cut(s) 1151
AspLEI GCGC 2 cut(s) 125, 370
AsuHPI GGTGA 2 cut(s) 328, 1141
AsuII TTCGAA 1 cut(s) 551
AvaI CYCGRG 1 cut(s) 1287
BanI GGYRCC 1 cut(s) 1346
BarI GAAGNNNNNNTAC 2 cut(s) 413, 445
BbvI GCAGC 1 cut(s) 262
BccI CCATC 2 cut(s) 313, 407
BceAI ACGGC 1 cut(s) 872
BciT130I CCWGG 1 cut(s) 846
BciVI GTATCC 1 cut(s) 1285
BclI TGATCA 1 cut(s) 492
BfaI CTAG 1 cut(s) 1178
BfrI CTTAAG 1 cut(s) 1370
BfuI GTATCC 1 cut(s) 1285
BglII AGATCT 1 cut(s) 1180
BisI GCNGC 2 cut(s) 251, 439
BlsI GCNGC 2 cut(s) 252, 440
BmcAI AGTACT 1 cut(s) 305
Bme1390I CCNGG 1 cut(s) 846
BmeT110I CYCGRG 1 cut(s) 1287
BmiI GGNNCC 1 cut(s) 1348
BmrFI CCNGG 1 cut(s) 846
BmrI ACTGGG 1 cut(s) 1283
BmsI GCATC 2 cut(s) 189, 1240
BmuI ACTGGG 1 cut(s) 1283
Bpu14I TTCGAA 1 cut(s) 551
BpuEI CTTGAG 4 cut(s) 38, 119, 252, 284
BsaJI CCNNGG 4 cut(s) 75, 229, 844, 1297
Bsc4I CCNNNNNNNGG 2 cut(s) 230, 894
Bse1I ACTGG 2 cut(s) 869, 1278
Bse3DI GCAATG 1 cut(s) 495
BseBI CCWGG 1 cut(s) 846
BseDI CCNNGG 4 cut(s) 75, 229, 844, 1297
BseGI GGATG 1 cut(s) 598
BseLI CCNNNNNNNGG 2 cut(s) 230, 894
BseMI GCAATG 1 cut(s) 495
BseMII CTCAG 4 cut(s) 117, 659, 822, 1034
BseNI ACTGG 2 cut(s) 869, 1278
BseRI GAGGAG 2 cut(s) 255, 1373
BseXI GCAGC 1 cut(s) 262
Bsh1236I CGCG 2 cut(s) 123, 438
BshFI GGCC 1 cut(s) 809
BshNI GGYRCC 1 cut(s) 1346
BsiHKCI CYCGRG 1 cut(s) 1287
BslI CCNNNNNNNGG 2 cut(s) 230, 894
BsmI GAATGC 1 cut(s) 672
BsnI GGCC 1 cut(s) 809
BsoBI CYCGRG 1 cut(s) 1287
Bsp119I TTCGAA 1 cut(s) 551
Bsp143I GATC 8 cut(s) 118, 220, 492, 580, 619, 937, 1180, 1338
Bsp19I CCATGG 1 cut(s) 229
BspACI CCGC 5 cut(s) 39, 438, 769, 1071, 1192
BspANI GGCC 1 cut(s) 809
BspCNI CTCAG 4 cut(s) 118, 660, 823, 1033
BspFNI CGCG 2 cut(s) 123, 438
BspHI TCATGA 1 cut(s) 69
BspLI GGNNCC 1 cut(s) 1348
BspPI GGATC 3 cut(s) 215, 575, 614
BspQI GCTCTTC 1 cut(s) 759
BspT104I TTCGAA 1 cut(s) 551
BspT107I GGYRCC 1 cut(s) 1346
BspTI CTTAAG 1 cut(s) 1370
BsrBI CCGCTC 1 cut(s) 769
BsrDI GCAATG 1 cut(s) 495
BsrI ACTGG 2 cut(s) 869, 1278
BssECI CCNNGG 4 cut(s) 75, 229, 844, 1297
BssMI GATC 8 cut(s) 118, 220, 492, 580, 619, 937, 1180, 1338
BssT1I CCWWGG 3 cut(s) 75, 229, 1297
Bst2UI CCWGG 1 cut(s) 846
Bst4CI ACNGT 5 cut(s) 570, 970, 1044, 1218, 1232
Bst6I CTCTTC 2 cut(s) 71, 759
BstAFI CTTAAG 1 cut(s) 1370
BstBI TTCGAA 1 cut(s) 551
BstC8I GCNNGC 1 cut(s) 744
BstDEI CTNAG 6 cut(s) 126, 417, 668, 831, 987, 1020
BstDSI CCRYGG 1 cut(s) 229
BstF5I GGATG 1 cut(s) 598
BstFNI CGCG 2 cut(s) 123, 438
BstHHI GCGC 2 cut(s) 125, 370
BstKTI GATC 8 cut(s) 121, 223, 495, 583, 622, 940, 1183, 1341
BstMBI GATC 8 cut(s) 118, 220, 492, 580, 619, 937, 1180, 1338
BstMWI GCNNNNNNNGC 3 cut(s) 256, 376, 925
BstNI CCWGG 1 cut(s) 846
BstSCI CCNGG 1 cut(s) 844
BstUI CGCG 2 cut(s) 123, 438
BstV1I GCAGC 1 cut(s) 262
BstX2I RGATCY 3 cut(s) 580, 619, 1180
BstXI CCANNNNNNTGG 1 cut(s) 864
BstYI RGATCY 3 cut(s) 580, 619, 1180
BsuI GTATCC 1 cut(s) 1285
BsuRI GGCC 1 cut(s) 809
BtgI CCRYGG 1 cut(s) 229
BtgZI GCGATG 1 cut(s) 1113
BtsCI GGATG 1 cut(s) 598
BtsIMutI CAGTG 2 cut(s) 575, 966
Cac8I GCNNGC 1 cut(s) 744
CciI TCATGA 1 cut(s) 69
CfoI GCGC 2 cut(s) 125, 370
CseI GACGC 1 cut(s) 444
Csp6I GTAC 8 cut(s) 97, 104, 157, 281, 304, 421, 750, 1080
CspCI CAANNNNNGTGG 2 cut(s) 955, 990
CviAII CATG 6 cut(s) 16, 70, 230, 475, 490, 1048
CviQI GTAC 8 cut(s) 97, 104, 157, 281, 304, 421, 750, 1080
DdeI CTNAG 6 cut(s) 126, 417, 668, 831, 987, 1020
DpnI GATC 8 cut(s) 120, 222, 494, 582, 621, 939, 1182, 1340
DpnII GATC 8 cut(s) 118, 220, 492, 580, 619, 937, 1180, 1338
DraI TTTAAA 1 cut(s) 793
EaeI YGGCCR 1 cut(s) 807
Eam1104I CTCTTC 2 cut(s) 71, 759
EarI CTCTTC 2 cut(s) 71, 759
EciI GGCGGA 1 cut(s) 28
Eco130I CCWWGG 3 cut(s) 75, 229, 1297
Eco57I CTGAAG 2 cut(s) 444, 982
Eco88I CYCGRG 1 cut(s) 1287
EcoRII CCWGG 1 cut(s) 844
EcoT14I CCWWGG 3 cut(s) 75, 229, 1297
ErhI CCWWGG 3 cut(s) 75, 229, 1297
FaeI CATG 6 cut(s) 19, 73, 233, 478, 493, 1051
FalI AAGNNNNNCTT 2 cut(s) 980, 1012
FatI CATG 6 cut(s) 15, 69, 229, 474, 489, 1047
FauI CCCGC 1 cut(s) 762
FbaI TGATCA 1 cut(s) 492
Fnu4HI GCNGC 2 cut(s) 251, 439
FokI GGATG 1 cut(s) 605
Fsp4HI GCNGC 2 cut(s) 251, 439
FspBI CTAG 1 cut(s) 1178
FspI TGCGCA 1 cut(s) 369
GlaI GCGC 2 cut(s) 124, 369
GluI GCNGC 2 cut(s) 251, 439
HaeIII GGCC 1 cut(s) 809
HgaI GACGC 1 cut(s) 444
HhaI GCGC 2 cut(s) 125, 370
Hin1II CATG 6 cut(s) 19, 73, 233, 478, 493, 1051
Hin6I GCGC 2 cut(s) 123, 368
HinP1I GCGC 2 cut(s) 123, 368
HindIII AAGCTT 1 cut(s) 257
HinfI GANTC 8 cut(s) 19, 35, 150, 235, 413, 645, 1108, 1385
HphI GGTGA 2 cut(s) 328, 1141
Hpy166II GTNNAC 3 cut(s) 159, 487, 1246
Hpy188I TCNGA 6 cut(s) 118, 240, 434, 463, 1029, 1260
Hpy188III TCNNGA 7 cut(s) 70, 269, 337, 623, 830, 1184, 1289
Hpy8I GTNNAC 3 cut(s) 159, 487, 1246
Hpy99I CGWCG 2 cut(s) 438, 1229
HpyAV CCTTC 4 cut(s) 404, 583, 1097, 1117
HpyCH4III ACNGT 5 cut(s) 570, 970, 1044, 1218, 1232
HpyCH4IV ACGT 4 cut(s) 423, 502, 872, 1327
HpyCH4V TGCA 6 cut(s) 202, 500, 573, 690, 905, 1253
HpyF10VI GCNNNNNNNGC 3 cut(s) 256, 376, 925
HpyF3I CTNAG 6 cut(s) 126, 417, 668, 831, 987, 1020
HpySE526I ACGT 4 cut(s) 423, 502, 872, 1327
Hsp92II CATG 6 cut(s) 19, 73, 233, 478, 493, 1051
HspAI GCGC 2 cut(s) 123, 368
Ksp22I TGATCA 1 cut(s) 492
Kzo9I GATC 8 cut(s) 118, 220, 492, 580, 619, 937, 1180, 1338
LguI GCTCTTC 1 cut(s) 759
LmnI GCTCC 1 cut(s) 247
Lsp1109I GCAGC 1 cut(s) 262
LweI GCATC 2 cut(s) 189, 1240
MaeI CTAG 1 cut(s) 1178
MaeII ACGT 4 cut(s) 423, 502, 872, 1327
MaeIII GTNAC 3 cut(s) 910, 977, 1282
MalI GATC 8 cut(s) 120, 222, 494, 582, 621, 939, 1182, 1340
MbiI CCGCTC 1 cut(s) 769
MboI GATC 8 cut(s) 118, 220, 492, 580, 619, 937, 1180, 1338
MfeI CAATTG 1 cut(s) 1061
MflI RGATCY 3 cut(s) 580, 619, 1180
MlyI GAGTC 2 cut(s) 159, 244
MmeI TCCRAC 2 cut(s) 457, 1245
MseI TTAA 7 cut(s) 521, 783, 792, 851, 1151, 1221, 1371
MslI CAYNNNNRTG 3 cut(s) 92, 803, 1151
MspCI CTTAAG 1 cut(s) 1370
MspR9I CCNGG 1 cut(s) 846
MunI CAATTG 1 cut(s) 1061
Mva1269I GAATGC 1 cut(s) 672
MvaI CCWGG 1 cut(s) 846
MvnI CGCG 2 cut(s) 123, 438
MwoI GCNNNNNNNGC 3 cut(s) 256, 376, 925
NcoI CCATGG 1 cut(s) 229
NdeII GATC 8 cut(s) 118, 220, 492, 580, 619, 937, 1180, 1338
NlaIII CATG 6 cut(s) 19, 73, 233, 478, 493, 1051
NlaIV GGNNCC 1 cut(s) 1348
NsbI TGCGCA 1 cut(s) 369
NspV TTCGAA 1 cut(s) 551
PagI TCATGA 1 cut(s) 69
PciSI GCTCTTC 1 cut(s) 759
PcsI WCGNNNNNNNCGW 1 cut(s) 28
PctI GAATGC 1 cut(s) 672
PfeI GAWTC 6 cut(s) 19, 35, 413, 645, 1108, 1385
PkrI GCNGC 2 cut(s) 252, 440
PleI GAGTC 2 cut(s) 158, 243
PpsI GAGTC 2 cut(s) 158, 243
PshBI ATTAAT 1 cut(s) 1151
Psp6I CCWGG 1 cut(s) 844
PspGI CCWGG 1 cut(s) 844
PspN4I GGNNCC 1 cut(s) 1348
PsuI RGATCY 3 cut(s) 580, 619, 1180
RsaI GTAC 8 cut(s) 98, 105, 158, 282, 305, 422, 751, 1081
RsaNI GTAC 8 cut(s) 97, 104, 157, 281, 304, 421, 750, 1080
RseI CAYNNNNRTG 3 cut(s) 92, 803, 1151
SapI GCTCTTC 1 cut(s) 759
SaqAI TTAA 7 cut(s) 521, 783, 792, 851, 1151, 1221, 1371
SatI GCNGC 2 cut(s) 251, 439
Sau3AI GATC 8 cut(s) 118, 220, 492, 580, 619, 937, 1180, 1338
ScaI AGTACT 1 cut(s) 305
SchI GAGTC 2 cut(s) 159, 244
ScrFI CCNGG 1 cut(s) 846
SfaNI GCATC 2 cut(s) 189, 1240
SfuI TTCGAA 1 cut(s) 551
SmiMI CAYNNNNRTG 3 cut(s) 92, 803, 1151
SmlI CTYRAG 5 cut(s) 53, 134, 267, 299, 1370
SmoI CTYRAG 5 cut(s) 53, 134, 267, 299, 1370
SsiI CCGC 5 cut(s) 39, 438, 769, 1071, 1192
SspI AATATT 1 cut(s) 601
SspMI CTAG 1 cut(s) 1178
StyD4I CCNGG 1 cut(s) 844
StyI CCWWGG 3 cut(s) 75, 229, 1297
TaaI ACNGT 5 cut(s) 570, 970, 1044, 1218, 1232
TaiI ACGT 4 cut(s) 426, 505, 875, 1330
TaqI TCGA 3 cut(s) 153, 551, 921
TatI WGTACW 5 cut(s) 96, 103, 156, 280, 303
TauI GCSGC 1 cut(s) 441
TfiI GAWTC 6 cut(s) 19, 35, 413, 645, 1108, 1385
Tru1I TTAA 7 cut(s) 521, 783, 792, 851, 1151, 1221, 1371
Tru9I TTAA 7 cut(s) 521, 783, 792, 851, 1151, 1221, 1371
TscAI CASTG 2 cut(s) 575, 973
TseI GCWGC 1 cut(s) 250
TspDTI ATGAA 5 cut(s) 58, 426, 777, 1068, 1128
TspGWI ACGGA 1 cut(s) 638
TspRI CASTG 2 cut(s) 575, 973
Vha464I CTTAAG 1 cut(s) 1370
VspI ATTAAT 1 cut(s) 1151
XapI RAATTY 1 cut(s) 679
XspI CTAG 1 cut(s) 1178
ZrmI AGTACT 1 cut(s) 305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.