Prupe.3G314100_v2.0.a1

Protein of unknown function (DUF3754)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0000718
Physical Location & Seq
Forward (+)
27155432 .. 27160161
4730 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G314100.1

Sequence Viewer

Length: 1476 bp
ATGAAGATGGGCAACAAGAAAGAGGTGATTCGATTAGAGCGTGAATCCGTGATTCAAGTTCTCAAGCCCAAACTCATCATGACCTTGGCCAACCTCATCGAACAAAGTTCTGACCGGGTTGAGTTTCTAAAGCTCTGCAAGAGAGTCGAGTACACAATTCGGGCTTGGTATCTCCTACAATTTGAAGATTTGATGCAACTATATTCCCTCTTTGACCCTGTACACGGGAGTCAGAAATTGGAGCAACAGAAGCTTTCTCCTGAAGAAATTGATGTACTTGAACAAAATTTCCTCAAGTACTTATTTCAGGTGATGGAAAAGAGTAATTTTAAGGTTGCAACTAATGAAGAAATTGAGGTTGCCCAGTCAGGGCAATATCTTCTAAATCTTCCCATCGTGGTTGATGAGTCTAAGCTTGACAAGCATCTTTTGAAGAAATACTTTGACAACCATCCTTACCCAAACATGCCAGATTTTGCTGATAAGTACATTATCTTCAGACGCGGAATTGGACTAGATAAGACAACCGATTACTTTATCATGGAGAAAGTGGACATTATCATTGCACGTTTCTGGGCATATCTTTTACGAGTAACTAAGGCTGAAAAGTTTTTTTCGAAAAGGCCAAGTGTACAATGCGAGAAGGATCCAAAAAAGGATGATGATCTTATCGCTGAACAAGATGAAGAGGACTTGTTTGTTGAACGAATCCGTCTTGAAAAAATGCAACTAAGTATTCCAAATTTGCTGAGCAAGAACACAATCCAAGAGCCAACTTTTGATAGGATTATCGTTGTTTACAGGCGAGCAAATAGTGGATCCGGAGATCAAGCAGAACGAGGAATATATGTTAAACATTTCAAACATATTCCAATGGCTGATATGGAGATTGTTCTTCCTGAGAAGAAGAACCCAGGATTAACGCCAATGGACTGGCTCACGTTCATTGGCTCTGCTGTAGTTGGGCTGGTTGCTGTTGTTACTTCGCTTGAAATGCCTAAAGTTGATCTTTGGGTCATTTTTGCAATTATTTCCACTGTGATTGGTTACTGTGCTAAGACATACTTCAGTTTTCAGCAGAACTTGGCTCAATATCAGAACTTGATAACACAGTCCATGTATGAAAAACAACTGGATAGCGGAAGGGGTACTCTTCTTCACTTGTGCGACGATGTGATTCAACAGGAAGTGAAGGAGGTGATCATTTCTTTTTATATATTAATGGAACAAGGGAAAGCTACTTTACCAGATCTGGATAAGTGGTGTGAAGAACTAATCAAAGAACAGTTTAACGACCACTGTAATTTCGATGTGGACGATGCGGTTCAGAAGTTGGAGAAATTGGGAATTGTTACTCGGGATACCCTTGGACGGTATAACTGCGTTGGGCTGAAACGTGCTAACGAGATCATTGGCACCACCACTGAGGAGGCGATGATGATTCTGCTTTCTCAAAACAGCCCTTGTCTTCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3754

Protein Analysis

492

Amino Acids

57.02

Weight (kDa)

5.33

Isoelectric Point (pI)

33.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1415
AccII CGCG 1 cut(s) 504
AccIII TCCGGA 1 cut(s) 821
AciI CCGC 3 cut(s) 504, 1140, 1322
AclWI GGATC 4 cut(s) 641, 654, 813, 826
AcoI YGGCCR 1 cut(s) 87
AcsI RAATTY 2 cut(s) 286, 742
AcuI CTGAAG 4 cut(s) 282, 481, 1051, 1454
AfaI GTAC 7 cut(s) 152, 222, 276, 299, 488, 633, 1150
AfiI CCNNNNNNNGG 3 cut(s) 224, 369, 1371
AgsI TTSAA 9 cut(s) 56, 185, 281, 433, 704, 719, 862, 992, 1181
AjnI CCWGG 1 cut(s) 913
AloI GAACNNNNNNTCC 2 cut(s) 273, 305
AluBI AGCT 4 cut(s) 133, 253, 415, 1238
AluI AGCT 4 cut(s) 133, 253, 415, 1238
AlwI GGATC 4 cut(s) 641, 654, 813, 826
Ama87I CYCGRG 1 cut(s) 1356
Aor13HI TCCGGA 1 cut(s) 821
AoxI GGCC 2 cut(s) 87, 623
ApoI RAATTY 2 cut(s) 286, 742
AseI ATTAAT 1 cut(s) 1220
AsuC2I CCSGG 1 cut(s) 116
AsuHPI GGTGA 3 cut(s) 37, 322, 1210
AsuII TTCGAA 1 cut(s) 617
AvaI CYCGRG 1 cut(s) 1356
BalI TGGCCA 1 cut(s) 89
BamHI GGATCC 2 cut(s) 646, 818
BanI GGYRCC 1 cut(s) 1415
BarI GAAGNNNNNNTAC 2 cut(s) 479, 511
BbsI GAAGAC 1 cut(s) 1460
BccI CCATC 3 cut(s) 307, 401, 459
BcgI CGANNNNNNTGC 2 cut(s) 127, 161
BciT130I CCWGG 1 cut(s) 915
BciVI GTATCC 1 cut(s) 1354
BclI TGATCA 1 cut(s) 1200
BcnI CCSGG 1 cut(s) 116
BfaI CTAG 1 cut(s) 515
BfmI CTRYAG 1 cut(s) 957
BfuI GTATCC 1 cut(s) 1354
BglII AGATCT 1 cut(s) 1249
BlpI GCTNAGC 1 cut(s) 749
BmcAI AGTACT 1 cut(s) 299
Bme1390I CCNGG 2 cut(s) 116, 915
BmeT110I CYCGRG 1 cut(s) 1356
BmiI GGNNCC 3 cut(s) 648, 820, 1417
BmrFI CCNGG 2 cut(s) 116, 915
BmrI ACTGGG 1 cut(s) 358
BmsI GCATC 3 cut(s) 183, 433, 1309
BmuI ACTGGG 1 cut(s) 358
BpiI GAAGAC 1 cut(s) 1460
Bpu1102I GCTNAGC 1 cut(s) 749
Bpu14I TTCGAA 1 cut(s) 617
BpuEI CTTGAG 2 cut(s) 47, 278
BpuMI CCSGG 1 cut(s) 116
BsaBI GATNNNNATC 1 cut(s) 663
BsaJI CCNNGG 3 cut(s) 84, 913, 1366
BsaWI WCCGGW 1 cut(s) 821
Bsc4I CCNNNNNNNGG 3 cut(s) 224, 369, 1371
Bse1I ACTGG 3 cut(s) 364, 938, 1137
Bse3DI GCAATG 1 cut(s) 561
Bse8I GATNNNNATC 1 cut(s) 663
BseAI TCCGGA 1 cut(s) 821
BseBI CCWGG 1 cut(s) 915
BseDI CCNNGG 3 cut(s) 84, 913, 1366
BseGI GGATG 2 cut(s) 451, 664
BseJI GATNNNNATC 1 cut(s) 663
BseLI CCNNNNNNNGG 3 cut(s) 224, 369, 1371
BseMI GCAATG 1 cut(s) 561
BseMII CTCAG 3 cut(s) 740, 891, 1416
BseNI ACTGG 3 cut(s) 364, 938, 1137
BseRI GAGGAG 1 cut(s) 1442
Bsh1236I CGCG 1 cut(s) 504
BshFI GGCC 2 cut(s) 89, 625
BshNI GGYRCC 1 cut(s) 1415
BsiHKCI CYCGRG 1 cut(s) 1356
BsiSI CCGG 2 cut(s) 115, 822
BslI CCNNNNNNNGG 3 cut(s) 224, 369, 1371
BsnI GGCC 2 cut(s) 89, 625
BsoBI CYCGRG 1 cut(s) 1356
Bsp119I TTCGAA 1 cut(s) 617
Bsp13I TCCGGA 1 cut(s) 821
Bsp1407I TGTACA 2 cut(s) 220, 631
Bsp143I GATC 8 cut(s) 646, 664, 818, 826, 1006, 1200, 1249, 1407
Bsp1720I GCTNAGC 1 cut(s) 749
BspACI CCGC 3 cut(s) 504, 1140, 1322
BspANI GGCC 2 cut(s) 89, 625
BspCNI CTCAG 3 cut(s) 741, 892, 1417
BspEI TCCGGA 1 cut(s) 821
BspFNI CGCG 1 cut(s) 504
BspHI TCATGA 1 cut(s) 78
BspLI GGNNCC 3 cut(s) 648, 820, 1417
BspPI GGATC 4 cut(s) 641, 654, 813, 826
BspT104I TTCGAA 1 cut(s) 617
BspT107I GGYRCC 1 cut(s) 1415
BsrDI GCAATG 1 cut(s) 561
BsrGI TGTACA 2 cut(s) 220, 631
BsrI ACTGG 3 cut(s) 364, 938, 1137
BssECI CCNNGG 3 cut(s) 84, 913, 1366
BssMI GATC 8 cut(s) 646, 664, 818, 826, 1006, 1200, 1249, 1407
BssT1I CCWWGG 2 cut(s) 84, 1366
Bst2UI CCWGG 1 cut(s) 915
Bst4CI ACNGT 6 cut(s) 1039, 1052, 1113, 1287, 1301, 1374
Bst6I CTCTTC 2 cut(s) 681, 1158
BstAUI TGTACA 2 cut(s) 220, 631
BstBI TTCGAA 1 cut(s) 617
BstC8I GCNNGC 1 cut(s) 807
BstDEI CTNAG 7 cut(s) 411, 597, 731, 749, 900, 1056, 1425
BstF5I GGATG 2 cut(s) 451, 664
BstFNI CGCG 1 cut(s) 504
BstKTI GATC 8 cut(s) 649, 667, 821, 829, 1009, 1203, 1252, 1410
BstMBI GATC 8 cut(s) 646, 664, 818, 826, 1006, 1200, 1249, 1407
BstMWI GCNNNNNNNGC 3 cut(s) 250, 421, 994
BstNI CCWGG 1 cut(s) 915
BstNSI RCATGY 1 cut(s) 469
BstSCI CCNGG 2 cut(s) 114, 913
BstSFI CTRYAG 1 cut(s) 957
BstUI CGCG 1 cut(s) 504
BstV2I GAAGAC 1 cut(s) 1460
BstX2I RGATCY 3 cut(s) 646, 818, 1249
BstXI CCANNNNNNTGG 1 cut(s) 933
BstYI RGATCY 3 cut(s) 646, 818, 1249
BsuI GTATCC 1 cut(s) 1354
BsuRI GGCC 2 cut(s) 89, 625
BtgZI GCGATG 1 cut(s) 1448
BtsCI GGATG 2 cut(s) 451, 664
BtsIMutI CAGTG 3 cut(s) 1035, 1297, 1422
Cac8I GCNNGC 1 cut(s) 807
CciI TCATGA 1 cut(s) 78
CseI GACGC 1 cut(s) 510
Csp6I GTAC 7 cut(s) 151, 221, 275, 298, 487, 632, 1149
CspCI CAANNNNNGTGG 2 cut(s) 1024, 1059
CviAII CATG 4 cut(s) 79, 466, 541, 1117
CviQI GTAC 7 cut(s) 151, 221, 275, 298, 487, 632, 1149
DdeI CTNAG 7 cut(s) 411, 597, 731, 749, 900, 1056, 1425
DpnI GATC 8 cut(s) 648, 666, 820, 828, 1008, 1202, 1251, 1409
DpnII GATC 8 cut(s) 646, 664, 818, 826, 1006, 1200, 1249, 1407
EaeI YGGCCR 1 cut(s) 87
Eam1104I CTCTTC 2 cut(s) 681, 1158
EarI CTCTTC 2 cut(s) 681, 1158
Eco130I CCWWGG 2 cut(s) 84, 1366
Eco57I CTGAAG 4 cut(s) 282, 481, 1051, 1454
Eco88I CYCGRG 1 cut(s) 1356
EcoRII CCWGG 1 cut(s) 913
EcoT14I CCWWGG 2 cut(s) 84, 1366
ErhI CCWWGG 2 cut(s) 84, 1366
FaeI CATG 4 cut(s) 82, 469, 544, 1120
FalI AAGNNNNNCTT 6 cut(s) 425, 457, 993, 1025, 1049, 1081
FatI CATG 4 cut(s) 78, 465, 540, 1116
FbaI TGATCA 1 cut(s) 1200
FokI GGATG 2 cut(s) 438, 671
FspBI CTAG 1 cut(s) 515
HaeIII GGCC 2 cut(s) 89, 625
HapII CCGG 2 cut(s) 115, 822
HgaI GACGC 1 cut(s) 510
Hin1II CATG 4 cut(s) 82, 469, 544, 1120
HindIII AAGCTT 2 cut(s) 251, 413
HinfI GANTC 9 cut(s) 28, 44, 52, 144, 229, 407, 708, 1177, 1441
HpaII CCGG 2 cut(s) 115, 822
HphI GGTGA 3 cut(s) 37, 322, 1210
Hpy166II GTNNAC 6 cut(s) 153, 223, 553, 632, 799, 1315
Hpy188I TCNGA 5 cut(s) 112, 234, 500, 1098, 1329
Hpy188III TCNNGA 7 cut(s) 79, 260, 716, 822, 899, 1253, 1358
Hpy8I GTNNAC 6 cut(s) 153, 223, 553, 632, 799, 1315
Hpy99I CGWCG 1 cut(s) 1172
HpyAV CCTTC 3 cut(s) 637, 1137, 1186
HpyCH4III ACNGT 6 cut(s) 1039, 1052, 1113, 1287, 1301, 1374
HpyCH4IV ACGT 3 cut(s) 568, 941, 1396
HpyCH4V TGCA 6 cut(s) 138, 196, 338, 566, 727, 1025
HpyF10VI GCNNNNNNNGC 3 cut(s) 250, 421, 994
HpyF3I CTNAG 7 cut(s) 411, 597, 731, 749, 900, 1056, 1425
HpySE526I ACGT 3 cut(s) 568, 941, 1396
Hsp92II CATG 4 cut(s) 82, 469, 544, 1120
Kpn2I TCCGGA 1 cut(s) 821
Ksp22I TGATCA 1 cut(s) 1200
Kzo9I GATC 8 cut(s) 646, 664, 818, 826, 1006, 1200, 1249, 1407
LmnI GCTCC 1 cut(s) 241
LweI GCATC 3 cut(s) 183, 433, 1309
MaeI CTAG 1 cut(s) 515
MaeII ACGT 3 cut(s) 568, 941, 1396
MaeIII GTNAC 4 cut(s) 592, 979, 1046, 1351
MalI GATC 8 cut(s) 648, 666, 820, 828, 1008, 1202, 1251, 1409
MboI GATC 8 cut(s) 646, 664, 818, 826, 1006, 1200, 1249, 1407
MflI RGATCY 3 cut(s) 646, 818, 1249
MlsI TGGCCA 1 cut(s) 89
MluNI TGGCCA 1 cut(s) 89
MlyI GAGTC 3 cut(s) 153, 238, 416
MmeI TCCRAC 1 cut(s) 1314
Mox20I TGGCCA 1 cut(s) 89
MroI TCCGGA 1 cut(s) 821
MscI TGGCCA 1 cut(s) 89
MseI TTAA 5 cut(s) 330, 852, 920, 1220, 1290
Msp20I TGGCCA 1 cut(s) 89
MspI CCGG 2 cut(s) 115, 822
MspR9I CCNGG 2 cut(s) 116, 915
MvaI CCWGG 1 cut(s) 915
MvnI CGCG 1 cut(s) 504
MwoI GCNNNNNNNGC 3 cut(s) 250, 421, 994
NciI CCSGG 1 cut(s) 116
NdeII GATC 8 cut(s) 646, 664, 818, 826, 1006, 1200, 1249, 1407
NlaIII CATG 4 cut(s) 82, 469, 544, 1120
NlaIV GGNNCC 3 cut(s) 648, 820, 1417
NspI RCATGY 1 cut(s) 469
NspV TTCGAA 1 cut(s) 617
PagI TCATGA 1 cut(s) 78
PcsI WCGNNNNNNNCGW 2 cut(s) 37, 1314
PfeI GAWTC 6 cut(s) 28, 44, 52, 708, 1177, 1441
PleI GAGTC 3 cut(s) 152, 237, 415
PpsI GAGTC 3 cut(s) 152, 237, 415
PshBI ATTAAT 1 cut(s) 1220
Psp6I CCWGG 1 cut(s) 913
PspGI CCWGG 1 cut(s) 913
PspN4I GGNNCC 3 cut(s) 648, 820, 1417
PsuI RGATCY 3 cut(s) 646, 818, 1249
RsaI GTAC 7 cut(s) 152, 222, 276, 299, 488, 633, 1150
RsaNI GTAC 7 cut(s) 151, 221, 275, 298, 487, 632, 1149
SaqAI TTAA 5 cut(s) 330, 852, 920, 1220, 1290
Sau3AI GATC 8 cut(s) 646, 664, 818, 826, 1006, 1200, 1249, 1407
ScaI AGTACT 1 cut(s) 299
SchI GAGTC 3 cut(s) 153, 238, 416
ScrFI CCNGG 2 cut(s) 116, 915
SfaNI GCATC 3 cut(s) 183, 433, 1309
SfcI CTRYAG 1 cut(s) 957
SfuI TTCGAA 1 cut(s) 617
SmlI CTYRAG 2 cut(s) 62, 293
SmoI CTYRAG 2 cut(s) 62, 293
SsiI CCGC 3 cut(s) 504, 1140, 1322
SspMI CTAG 1 cut(s) 515
StyD4I CCNGG 2 cut(s) 114, 913
StyI CCWWGG 2 cut(s) 84, 1366
TaaI ACNGT 6 cut(s) 1039, 1052, 1113, 1287, 1301, 1374
TaiI ACGT 3 cut(s) 571, 944, 1399
TaqI TCGA 5 cut(s) 31, 99, 147, 617, 1308
TatI WGTACW 6 cut(s) 150, 220, 274, 297, 486, 631
TfiI GAWTC 6 cut(s) 28, 44, 52, 708, 1177, 1441
Tru1I TTAA 5 cut(s) 330, 852, 920, 1220, 1290
Tru9I TTAA 5 cut(s) 330, 852, 920, 1220, 1290
TscAI CASTG 3 cut(s) 1042, 1304, 1429
TspDTI ATGAA 5 cut(s) 17, 360, 699, 934, 1137
TspGWI ACGGA 2 cut(s) 37, 701
TspRI CASTG 3 cut(s) 1042, 1304, 1429
VspI ATTAAT 1 cut(s) 1220
XapI RAATTY 2 cut(s) 286, 742
XceI RCATGY 1 cut(s) 469
XspI CTAG 1 cut(s) 515
ZrmI AGTACT 1 cut(s) 299
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.