Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0000311

Physical Location & Seq

Genomic Coordinates Reverse (-)

32433205 .. 32435510

Gene Structure 2306 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1196900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1551 bp

ATGGCTAAGCTTTTTGTTTTCTCTCTTTGCTTGCTTCTTATAATCAACTGCTCCCTAGGGTCCCGCCAGCAGTTTCAGCAGCAGAATGAGTGCCAGTTCAACCAGCTCCAGTCCCGGGAGCCCGACAACCAGATTCAATCCGAGGCTGGTCTGACCGAGTCCTGGAACCCCAACGACCAGCAGTTCCAATGCGCCGGCGTTGCCATGGTTCGGCGCACCATCCAACCCAACGGCCTTCACTTGCCTTCCTTCCTCAACTCTCCGCAACTCATCTACATTGTTCAAGGTAGGGGTGTGTTGGGTGTGGCGTTCCCCGGCTGTCCTGAGACGTTCGAGGATTCTCAGGACCGGCAATTCCAGCACCAACAACAATTCCAGCCGTTCCAGCCCTCCCGACGACACGGAGGACAGGGACAACAACAACAGCAAAGCGAGGAGAACCAAGACCGCCACCAGAAGGTCCGACACATCAGAGAGGGTGATATCATCGCCTTGCCCGCCGGTGTCGCTTACTGGAGCTACAACAACGGCAACCAGGATCTCGTTGCTATCTCCCTCCTTGACTTCAGCAACGAGCAGAACCAGCTCGACCAACAGCCTAGGAGATTTTATCTAGCTGGCAACCCACAAGACGAGTTCAGCCAGCAACAAGGCCAACGGTATCAGCAGGGACGCCAACAGCAACAGCAGGGCCGCCAACAGCAGCAGCAGGGCCAGGGTGGACAGCAAGAACGGGGCCAACAAGGCCGGCGTGGACAGCAGCAACAGGGGGGCAACGGCAAAAACCTCTTTGCCGGATTCGACACTCAGATGCTCGCTCAGGCTCTGAATGTCGACGAAGAGATCGTCAACAGGCTCCAGGGACGGAACGACGACAGGAACGAGATTGTCCAGGTGCAGGGCCAGCTCGACTTTGTCAGCCCATTCAGCCAGGGCGAGCAGGAAGGGAGACGCCAGATGGGAGGAGGAGGCCGCGCCAACGGTTTGGAGGAGACATTCTGCAGCATGAGGCTCAAGGAGAACATCCGCGACCCTTCTAACGCCGACTTCTACAGCCCTCAGGCTGGCCGCGTTTCCGTCGTCAACAGCGGCACCCTCCCCATCCTCAGCGCTCTCCGCCTCAGCGCCGAGCATGGCGTCCTTTATAACAATGCCATATACAGTCCATACTGGAACCTCAACGCGAACGAACTTGTATACGTGATCAGAGGCAATGCTAGGGTTCAGGTGGTGAACGAGAACGGAGACGCCATCTTGGACGACCAGGTCAACGAGGGACAGGTGTTCTTGATTCCCCAGAACCACGCCGTCATCACCCAGGCAGGCAACCAGGGATTCGAGTACATTTCCTTCAGGACGAATGACTTGGCCCTCATTAACACCATGGCAGGGAGGACTGCCGTGTTGAGGGCGATCCCGGAGGATGTGCTTCGCAATGCCTTCCAGATTGACCGCCAACAGGTCAGGGATCTCAAGAACAACAGGCAGGAGACCCGAGTGTTGAGTGCCAGCTCTTCTTCCTCACGCCGACCATGGTCGATCATGGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0007275 GO:0008150 GO:0009719 GO:0009725 GO:0009735 GO:0009737 GO:0009791 GO:0009987 GO:0010033 GO:0010154 GO:0010431 GO:0021700 GO:0022414 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032504 GO:0032870 GO:0033095 GO:0033993 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045735 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0051716 GO:0061458 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071695 GO:0097305 GO:0097306 GO:0097708 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cupin_1

Protein Analysis

517

Amino Acids

58.28

Weight (kDa)

5.9

Isoelectric Point (pI)

57.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cupin_1	PF00190	39 - 209	4.6e-28	Cupin
Cupin_1	PF00190	341 - 489	6.6e-37	Cupin

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000311)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G03880 AT1G03890 AT4G28520 AT4G28520 AT4G28520 AT4G28520 AT4G28520 AT5G44120 AT5G44120 AT5G44120
fragaria_vesca	FvH4_2g21390 FvH4_2g21390 FvH4_2g21400 FvH4_2g21410 FvH4_2g21410 FvH4_2g21410 FvH4_2g21420 FvH4_2g21420 FvH4_2g21420 FvH4_2g21420 FvH4_2g21430 FvH4_2g21430 FvH4_2g21430 FvH4_2g21430 FvH4_2g21430 FvH4_2g21430 FvH4_2g21440 FvH4_3g19080
malus_domestica	MD01G1024700.v1.1 MD05G1196800.v1.1 MD05G1196900.v1.1 MD17G1078200.v1.1
prunus_persica	Prupe.8G223300_v2.0.a1 Prupe.8G223400_v2.0.a1 Prupe.8G223400_v2.0.a1
pyrus_communis	pycom05g18380 pycom05g18390
rosa_chinensis	RchiOBHm_Chr6g0287991 RchiOBHm_Chr6g0288001 RchiOBHm_Chr6g0288021 RchiOBHm_Chr6g0288031 RchiOBHm_Chr6g0288041 RchiOBHm_Chr6g0288051 RchiOBHm_Chr6g0288071
rosa_laevigata	RLG00000012441 RLG00000012443 RLG00000012444 RLG00000012446 RLG00000012447 RLG00000012448
rosa_multiflora	Rmu_co8147412.1_g000001 Rmu_co8487931.1_g000001 Rmu_co8505755.1_g000001 Rmu_sc0001117.1_g000003 Rmu_sc0001117.1_g000005 Rmu_sc0001117.1_g000011 Rmu_sc0001117.1_g000013 Rmu_sc0005266.1_g000010 Rmu_sc0010960.1_g000004
rosa_roxburghii	Rroxscaffold_3G00252330 Rroxscaffold_7G00180530 Rroxscaffold_7G00180550 Rroxscaffold_7G00180560 Rroxscaffold_7G00180570 Rroxscaffold_7G00180580 Rroxscaffold_7G00180590
rosa_rugosa	Rorug06G0041100 Rorug06G0041200 Rorug06G0041200 Rorug06G0041400 Rorug06G0193700 Rorug06G0193700 Rorug06G0193700 Rorug06G0193800 Rorug06G0193800 Rorug06G0193900 Rorug06G0194000 Rorug06G0194100
rosa_samantha	Rh6AG303300 Rh6AG303400 Rh6AG303500 Rh6AG303700 Rh6AG303800 Rh6AG303900 Rh6AG304100 Rh6AG304200 Rh6BG309500 Rh6BG309600 Rh6BG309700 Rh6BG309900 Rh6BG310000 Rh6BG310100 Rh6BG310200 Rh6CG299600 Rh6CG299800 Rh6CG300000 Rh6CG300200 Rh6CG300300 Rh6CG317100 Rh6CG317300 Rh6CG317500 Rh6CG317700 Rh6CG317800 Rh6CG318200 Rh6DG302000 Rh6DG302100 Rh6DG302300 Rh6DG302500 Rh6DG302600 Rh6DG302700 Rh6DG303100
rosa_wichuraiana	Rw6G026230 Rw6G026240 Rw6G026260 Rw6G026280 Rw6G026300 Rw6G026320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	41, 1146
AasI	GACNNNNNNGTC	1 cut(s)	1265
AccB1I	GGYRCC	1 cut(s)	1091
AccI	GTMKAC	2 cut(s)	834, 1197
AccII	CGCG	4 cut(s)	975, 1029, 1071, 1184
AclWI	GGATC	3 cut(s)	546, 1408, 1476
AcoI	YGGCCR	1 cut(s)	1066
AcuI	CTGAAG	2 cut(s)	550, 1336
AcyI	GRCGYC	4 cut(s)	673, 952, 1137, 1248
AfaI	GTAC	1 cut(s)	1343
AfeI	AGCGCT	1 cut(s)	1111
AfiI	CCNNNNNNNGG	9 cut(s)	115, 162, 210, 457, 898, 1064, 1389, 1407, 1459
AgsI	TTSAA	3 cut(s)	100, 137, 284
AjnI	CCWGG	9 cut(s)	161, 534, 714, 858, 891, 930, 1263, 1317, 1329
AluBI	AGCT	7 cut(s)	10, 106, 519, 586, 617, 907, 1512
AluI	AGCT	7 cut(s)	10, 106, 519, 586, 617, 907, 1512
Alw26I	GTCTC	5 cut(s)	320, 943, 986, 1239, 1484
AlwI	GGATC	3 cut(s)	546, 1408, 1476
AlwNI	CAGNNNCTG	1 cut(s)	826
Ama87I	CYCGRG	2 cut(s)	114, 1494
Aor51HI	AGCGCT	1 cut(s)	1111
ApeKI	GCWGC	5 cut(s)	79, 703, 706, 760, 1002
ArsI	GACNNNNNNTTYG	2 cut(s)	831, 863
AspA2I	CCTAGG	2 cut(s)	55, 599
AspLEI	GCGC	5 cut(s)	194, 216, 977, 1112, 1127
AspS9I	GGNCC	8 cut(s)	60, 346, 460, 691, 712, 736, 901, 1369
AsuC2I	CCSGG	4 cut(s)	115, 116, 315, 1418
AsuHPI	GGTGA	3 cut(s)	491, 1243, 1306
AvaI	CYCGRG	2 cut(s)	114, 1494
AvaII	GGWCC	3 cut(s)	60, 346, 460
AvrII	CCTAGG	2 cut(s)	55, 599
AxyI	CCTNAGG	1 cut(s)	1059
BanI	GGYRCC	1 cut(s)	1091
BanII	GRGCYC	1 cut(s)	123
BarI	GAAGNNNNNNTAC	2 cut(s)	1334, 1366
BbvCI	CCTCAGC	2 cut(s)	1106, 1121
BbvI	GCAGC	5 cut(s)	91, 715, 718, 772, 1014
BccI	CCATC	4 cut(s)	227, 952, 1109, 1259
BceAI	ACGGC	6 cut(s)	247, 364, 544, 793, 1292, 1385
BciT130I	CCWGG	9 cut(s)	163, 536, 716, 860, 893, 932, 1265, 1319, 1331
BclI	TGATCA	1 cut(s)	1203
BcnI	CCSGG	4 cut(s)	115, 116, 315, 1418
BcoDI	GTCTC	5 cut(s)	320, 943, 986, 1239, 1484
BfaI	CTAG	4 cut(s)	56, 600, 614, 1218
BfmI	CTRYAG	2 cut(s)	1000, 1051
BfoI	RGCGCY	2 cut(s)	1113, 1128
BglI	GCCNNNNNGGC	1 cut(s)	744
BisI	GCNGC	9 cut(s)	80, 694, 704, 707, 761, 973, 1003, 1069, 1090
BlnI	CCTAGG	2 cut(s)	55, 599
BlpI	GCTNAGC	1 cut(s)	6
BlsI	GCNGC	9 cut(s)	81, 695, 705, 708, 762, 974, 1004, 1070, 1091
Bme18I	GGWCC	3 cut(s)	60, 346, 460
BmeT110I	CYCGRG	2 cut(s)	114, 1494
BmgT120I	GGNCC	8 cut(s)	60, 346, 460, 691, 712, 736, 901, 1369
BmiI	GGNNCC	8 cut(s)	61, 62, 120, 167, 737, 857, 1093, 1175
BmsI	GCATC	1 cut(s)	801
BoxI	GACNNNNGTC	1 cut(s)	1534
BpmI	CTGGAG	3 cut(s)	92, 535, 842
Bpu10I	CCTNAGC	3 cut(s)	819, 1106, 1121
Bpu1102I	GCTNAGC	1 cut(s)	6
BpuEI	CTTGAG	2 cut(s)	998, 1457
BpuMI	CCSGG	4 cut(s)	115, 116, 315, 1418
BsaAI	YACGTR	1 cut(s)	1201
BsaHI	GRCGYC	4 cut(s)	673, 952, 1137, 1248
BsaI	GGTCTC	1 cut(s)	1484
Bsc4I	CCNNNNNNNGG	9 cut(s)	115, 162, 210, 457, 898, 1064, 1389, 1407, 1459
Bse118I	RCCGGY	4 cut(s)	194, 348, 500, 747
Bse1I	ACTGG	4 cut(s)	94, 109, 518, 1175
Bse21I	CCTNAGG	1 cut(s)	1059
Bse3DI	GCAATG	2 cut(s)	1219, 1441
BseBI	CCWGG	9 cut(s)	163, 536, 716, 860, 893, 932, 1265, 1319, 1331
BseGI	GGATG	4 cut(s)	219, 1023, 1101, 1429
BseLI	CCNNNNNNNGG	9 cut(s)	115, 162, 210, 457, 898, 1064, 1389, 1407, 1459
BseMI	GCAATG	2 cut(s)	1219, 1441
BseMII	CTCAG	7 cut(s)	315, 356, 821, 833, 1073, 1120, 1135
BseNI	ACTGG	4 cut(s)	94, 109, 518, 1175
BseRI	GAGGAG	4 cut(s)	449, 978, 981, 1004
BseXI	GCAGC	5 cut(s)	91, 715, 718, 772, 1014
BsgI	GTGCAG	1 cut(s)	917
Bsh1236I	CGCG	4 cut(s)	975, 1029, 1071, 1184
BshNI	GGYRCC	1 cut(s)	1091
BsiHKCI	CYCGRG	2 cut(s)	114, 1494
BsiSI	CCGG	8 cut(s)	115, 195, 315, 349, 501, 748, 795, 1418
BslFI	GGGAC	6 cut(s)	46, 97, 426, 684, 876, 1290
BslI	CCNNNNNNNGG	9 cut(s)	115, 162, 210, 457, 898, 1064, 1389, 1407, 1459
BsmAI	GTCTC	5 cut(s)	320, 943, 986, 1239, 1484
BsmBI	CGTCTC	3 cut(s)	320, 943, 1239
BsmFI	GGGAC	6 cut(s)	46, 97, 426, 684, 876, 1290
Bso31I	GGTCTC	1 cut(s)	1484
BsoBI	CYCGRG	2 cut(s)	114, 1494
Bsp1286I	GDGCHC	1 cut(s)	123
Bsp143I	GATC	6 cut(s)	538, 843, 1203, 1413, 1468, 1539
Bsp1720I	GCTNAGC	1 cut(s)	6
Bsp19I	CCATGG	3 cut(s)	204, 1383, 1532
BspCNI	CTCAG	7 cut(s)	316, 355, 820, 832, 1072, 1119, 1134
BspFNI	CGCG	4 cut(s)	975, 1029, 1071, 1184
BspLI	GGNNCC	8 cut(s)	61, 62, 120, 167, 737, 857, 1093, 1175
BspMAI	CTGCAG	1 cut(s)	1004
BspPI	GGATC	3 cut(s)	546, 1408, 1476
BspQI	GCTCTTC	1 cut(s)	1519
BspT107I	GGYRCC	1 cut(s)	1091
BspTNI	GGTCTC	1 cut(s)	1484
BsrDI	GCAATG	2 cut(s)	1219, 1441
BsrFI	RCCGGY	4 cut(s)	194, 348, 500, 747
BsrI	ACTGG	4 cut(s)	94, 109, 518, 1175
BssAI	RCCGGY	4 cut(s)	194, 348, 500, 747
BssMI	GATC	6 cut(s)	538, 843, 1203, 1413, 1468, 1539
BssNAI	GTATAC	1 cut(s)	1198
BssNI	GRCGYC	4 cut(s)	673, 952, 1137, 1248
BssT1I	CCWWGG	5 cut(s)	55, 204, 599, 1383, 1532
Bst1107I	GTATAC	1 cut(s)	1198
Bst2UI	CCWGG	9 cut(s)	163, 536, 716, 860, 893, 932, 1265, 1319, 1331
Bst4CI	ACNGT	3 cut(s)	660, 983, 1163
Bst6I	CTCTTC	2 cut(s)	834, 1519
BstACI	GRCGYC	4 cut(s)	673, 952, 1137, 1248
BstBAI	YACGTR	1 cut(s)	1201
BstDEI	CTNAG	8 cut(s)	6, 324, 342, 807, 819, 1059, 1106, 1121
BstDSI	CCRYGG	3 cut(s)	204, 1383, 1532
BstF5I	GGATG	4 cut(s)	219, 1023, 1101, 1429
BstFNI	CGCG	4 cut(s)	975, 1029, 1071, 1184
BstH2I	RGCGCY	2 cut(s)	1113, 1128
BstHHI	GCGC	5 cut(s)	194, 216, 977, 1112, 1127
BstKTI	GATC	6 cut(s)	541, 846, 1206, 1416, 1471, 1542
BstMAI	GTCTC	5 cut(s)	320, 943, 986, 1239, 1484
BstMBI	GATC	6 cut(s)	538, 843, 1203, 1413, 1468, 1539
BstNI	CCWGG	9 cut(s)	163, 536, 716, 860, 893, 932, 1265, 1319, 1331
BstPAI	GACNNNNGTC	1 cut(s)	1534
BstSFI	CTRYAG	2 cut(s)	1000, 1051
BstUI	CGCG	4 cut(s)	975, 1029, 1071, 1184
BstV1I	GCAGC	5 cut(s)	91, 715, 718, 772, 1014
BstX2I	RGATCY	2 cut(s)	538, 1468
BstXI	CCANNNNNNTGG	1 cut(s)	985
BstYI	RGATCY	2 cut(s)	538, 1468
BstZ17I	GTATAC	1 cut(s)	1198
Bsu36I	CCTNAGG	1 cut(s)	1059
BtgI	CCRYGG	3 cut(s)	204, 1383, 1532
BtgZI	GCGATG	1 cut(s)	472
BtsCI	GGATG	4 cut(s)	219, 1023, 1101, 1429
CaiI	CAGNNNCTG	1 cut(s)	826
CfoI	GCGC	5 cut(s)	194, 216, 977, 1112, 1127
Cfr10I	RCCGGY	4 cut(s)	194, 348, 500, 747
Cfr13I	GGNCC	8 cut(s)	60, 346, 460, 691, 712, 736, 901, 1369
Cfr9I	CCCGGG	1 cut(s)	114
CseI	GACGC	4 cut(s)	681, 960, 1126, 1256
CsiI	ACCWGGT	1 cut(s)	1263
Csp6I	GTAC	1 cut(s)	1342
CviAII	CATG	7 cut(s)	205, 1006, 1133, 1384, 1533, 1543, 1548
CviQI	GTAC	1 cut(s)	1342
DdeI	CTNAG	8 cut(s)	6, 324, 342, 807, 819, 1059, 1106, 1121
DpnI	GATC	6 cut(s)	540, 845, 1205, 1415, 1470, 1541
DpnII	GATC	6 cut(s)	538, 843, 1203, 1413, 1468, 1539
DrdI	GACNNNNNNGTC	1 cut(s)	1265
DseDI	GACNNNNNNGTC	1 cut(s)	1265
EaeI	YGGCCR	1 cut(s)	1066
Eam1104I	CTCTTC	2 cut(s)	834, 1519
EarI	CTCTTC	2 cut(s)	834, 1519
EciI	GGCGGA	1 cut(s)	1106
Eco130I	CCWWGG	5 cut(s)	55, 204, 599, 1383, 1532
Eco24I	GRGCYC	1 cut(s)	123
Eco31I	GGTCTC	1 cut(s)	1484
Eco32I	GATATC	1 cut(s)	484
Eco47I	GGWCC	3 cut(s)	60, 346, 460
Eco47III	AGCGCT	1 cut(s)	1111
Eco57I	CTGAAG	2 cut(s)	550, 1336
Eco81I	CCTNAGG	1 cut(s)	1059
Eco88I	CYCGRG	2 cut(s)	114, 1494
EcoO109I	RGGNCCY	1 cut(s)	60
EcoRII	CCWGG	9 cut(s)	161, 534, 714, 858, 891, 930, 1263, 1317, 1329
EcoRV	GATATC	1 cut(s)	484
EcoT14I	CCWWGG	5 cut(s)	55, 204, 599, 1383, 1532
EcoT38I	GRGCYC	1 cut(s)	123
ErhI	CCWWGG	5 cut(s)	55, 204, 599, 1383, 1532
Esp3I	CGTCTC	3 cut(s)	320, 943, 1239
FaeI	CATG	7 cut(s)	208, 1009, 1136, 1387, 1536, 1546, 1551
FaqI	GGGAC	6 cut(s)	46, 97, 426, 684, 876, 1290
FatI	CATG	7 cut(s)	204, 1005, 1132, 1383, 1532, 1542, 1547
FauI	CCCGC	2 cut(s)	71, 505
FbaI	TGATCA	1 cut(s)	1203
FblI	GTMKAC	2 cut(s)	834, 1197
Fnu4HI	GCNGC	9 cut(s)	80, 694, 704, 707, 761, 973, 1003, 1069, 1090
FokI	GGATG	4 cut(s)	206, 1010, 1088, 1436
FriOI	GRGCYC	1 cut(s)	123
Fsp4HI	GCNGC	9 cut(s)	80, 694, 704, 707, 761, 973, 1003, 1069, 1090
FspBI	CTAG	4 cut(s)	56, 600, 614, 1218
GlaI	GCGC	5 cut(s)	193, 215, 976, 1111, 1126
GluI	GCNGC	9 cut(s)	80, 694, 704, 707, 761, 973, 1003, 1069, 1090
GsuI	CTGGAG	3 cut(s)	92, 535, 842
HaeII	RGCGCY	2 cut(s)	1113, 1128
HapII	CCGG	8 cut(s)	115, 195, 315, 349, 501, 748, 795, 1418
HgaI	GACGC	4 cut(s)	681, 960, 1126, 1256
HhaI	GCGC	5 cut(s)	194, 216, 977, 1112, 1127
Hin1I	GRCGYC	4 cut(s)	673, 952, 1137, 1248
Hin1II	CATG	7 cut(s)	208, 1009, 1136, 1387, 1536, 1546, 1551
Hin6I	GCGC	5 cut(s)	192, 214, 975, 1110, 1125
HinP1I	GCGC	5 cut(s)	192, 214, 975, 1110, 1125
HincII	GTYRAC	4 cut(s)	835, 850, 1084, 1270
HindII	GTYRAC	4 cut(s)	835, 850, 1084, 1270
HindIII	AAGCTT	1 cut(s)	8
HinfI	GANTC	6 cut(s)	133, 158, 338, 798, 1291, 1335
HpaII	CCGG	8 cut(s)	115, 195, 315, 349, 501, 748, 795, 1418
HphI	GGTGA	3 cut(s)	491, 1243, 1306
Hpy166II	GTNNAC	8 cut(s)	722, 755, 835, 850, 1084, 1198, 1234, 1270
Hpy188I	TCNGA	7 cut(s)	142, 153, 464, 473, 810, 828, 1208
Hpy188III	TCNNGA	7 cut(s)	323, 344, 393, 1288, 1354, 1444, 1474
Hpy8I	GTNNAC	8 cut(s)	722, 755, 835, 850, 1084, 1198, 1234, 1270
Hpy99I	CGWCG	4 cut(s)	399, 839, 875, 1082
HpyAV	CCTTC	8 cut(s)	245, 255, 259, 451, 938, 1044, 1360, 1450
HpyCH4III	ACNGT	3 cut(s)	660, 983, 1163
HpyCH4IV	ACGT	2 cut(s)	329, 1200
HpyCH4V	TGCA	2 cut(s)	898, 1002
HpyF3I	CTNAG	8 cut(s)	6, 324, 342, 807, 819, 1059, 1106, 1121
HpySE526I	ACGT	2 cut(s)	329, 1200
Hsp92I	GRCGYC	4 cut(s)	673, 952, 1137, 1248
Hsp92II	CATG	7 cut(s)	208, 1009, 1136, 1387, 1536, 1546, 1551
HspAI	GCGC	5 cut(s)	192, 214, 975, 1110, 1125
KflI	GGGWCCC	1 cut(s)	60
KroI	GCCGGC	2 cut(s)	194, 747
KroNI	GCCGGC	2 cut(s)	196, 749
Ksp22I	TGATCA	1 cut(s)	1203
Kzo9I	GATC	6 cut(s)	538, 843, 1203, 1413, 1468, 1539
LguI	GCTCTTC	1 cut(s)	1519
LmnI	GCTCC	5 cut(s)	56, 111, 118, 516, 861
Lsp1109I	GCAGC	5 cut(s)	91, 715, 718, 772, 1014
LweI	GCATC	1 cut(s)	801
MabI	ACCWGGT	1 cut(s)	1263
MaeI	CTAG	4 cut(s)	56, 600, 614, 1218
MaeII	ACGT	2 cut(s)	329, 1200
MalI	GATC	6 cut(s)	540, 845, 1205, 1415, 1470, 1541
MboI	GATC	6 cut(s)	538, 843, 1203, 1413, 1468, 1539
MboII	GAAGA	3 cut(s)	851, 1506, 1509
MflI	RGATCY	2 cut(s)	538, 1468
MhlI	GDGCHC	1 cut(s)	123
MluCI	AATT	2 cut(s)	353, 371
MlyI	GAGTC	1 cut(s)	167
MmeI	TCCRAC	2 cut(s)	247, 487
MreI	CGCCGGCG	1 cut(s)	194
MroNI	GCCGGC	2 cut(s)	194, 747
MseI	TTAA	1 cut(s)	1377
MslI	CAYNNNNRTG	1 cut(s)	809
MspA1I	CMGCKG	1 cut(s)	1089
MspI	CCGG	8 cut(s)	115, 195, 315, 349, 501, 748, 795, 1418
MvaI	CCWGG	9 cut(s)	163, 536, 716, 860, 893, 932, 1265, 1319, 1331
MvnI	CGCG	4 cut(s)	975, 1029, 1071, 1184
NaeI	GCCGGC	2 cut(s)	196, 749
NciI	CCSGG	4 cut(s)	115, 116, 315, 1418
NcoI	CCATGG	3 cut(s)	204, 1383, 1532
NdeII	GATC	6 cut(s)	538, 843, 1203, 1413, 1468, 1539
NgoMIV	GCCGGC	2 cut(s)	194, 747
NlaIII	CATG	7 cut(s)	208, 1009, 1136, 1387, 1536, 1546, 1551
NlaIV	GGNNCC	8 cut(s)	61, 62, 120, 167, 737, 857, 1093, 1175
NmeAIII	GCCGAG	1 cut(s)	1153
PciSI	GCTCTTC	1 cut(s)	1519
PcsI	WCGNNNNNNNCGW	2 cut(s)	843, 879
PdiI	GCCGGC	2 cut(s)	196, 749
PfeI	GAWTC	5 cut(s)	133, 338, 798, 1291, 1335
PflFI	GACNNNGTC	3 cut(s)	157, 914, 1265
PfoI	TCCNGGA	2 cut(s)	161, 1416
PkrI	GCNGC	9 cut(s)	81, 695, 705, 708, 762, 974, 1004, 1070, 1091
PleI	GAGTC	1 cut(s)	166
PpsI	GAGTC	1 cut(s)	166
Ppu21I	YACGTR	1 cut(s)	1201
PpuMI	RGGWCCY	1 cut(s)	60
PshAI	GACNNNNGTC	1 cut(s)	1534
PsiI	TTATAA	2 cut(s)	41, 1146
Psp5II	RGGWCCY	1 cut(s)	60
Psp6I	CCWGG	9 cut(s)	161, 534, 714, 858, 891, 930, 1263, 1317, 1329
PspGI	CCWGG	9 cut(s)	161, 534, 714, 858, 891, 930, 1263, 1317, 1329
PspN4I	GGNNCC	8 cut(s)	61, 62, 120, 167, 737, 857, 1093, 1175
PspPI	GGNCC	8 cut(s)	60, 346, 460, 691, 712, 736, 901, 1369
PspPPI	RGGWCCY	1 cut(s)	60
PstI	CTGCAG	1 cut(s)	1004
PstNI	CAGNNNCTG	1 cut(s)	826
PsuI	RGATCY	2 cut(s)	538, 1468
PsyI	GACNNNGTC	3 cut(s)	157, 914, 1265
RsaI	GTAC	1 cut(s)	1343
RsaNI	GTAC	1 cut(s)	1342
RseI	CAYNNNNRTG	1 cut(s)	809
SalI	GTCGAC	1 cut(s)	833
SapI	GCTCTTC	1 cut(s)	1519
SaqAI	TTAA	1 cut(s)	1377
SatI	GCNGC	9 cut(s)	80, 694, 704, 707, 761, 973, 1003, 1069, 1090
Sau3AI	GATC	6 cut(s)	538, 843, 1203, 1413, 1468, 1539
Sau96I	GGNCC	8 cut(s)	60, 346, 460, 691, 712, 736, 901, 1369
SchI	GAGTC	1 cut(s)	167
SduI	GDGCHC	1 cut(s)	123
SexAI	ACCWGGT	1 cut(s)	1263
SfaNI	GCATC	1 cut(s)	801
SfcI	CTRYAG	2 cut(s)	1000, 1051
SfiI	GGCCNNNNNGGCC	1 cut(s)	744
SgrAI	CRCCGGYG	2 cut(s)	194, 500
SinI	GGWCC	3 cut(s)	60, 346, 460
SmaI	CCCGGG	1 cut(s)	116
SmiMI	CAYNNNNRTG	1 cut(s)	809
SmlI	CTYRAG	2 cut(s)	1013, 1472
SmoI	CTYRAG	2 cut(s)	1013, 1472
Sse9I	AATT	2 cut(s)	353, 371
SspMI	CTAG	4 cut(s)	56, 600, 614, 1218
StyI	CCWWGG	5 cut(s)	55, 204, 599, 1383, 1532
TaaI	ACNGT	3 cut(s)	660, 983, 1163
TaiI	ACGT	2 cut(s)	332, 1203
TaqI	TCGA	7 cut(s)	333, 588, 801, 834, 909, 1338, 1538
TaqII	GACCGA	1 cut(s)	170
TasI	AATT	2 cut(s)	353, 371
TatI	WGTACW	1 cut(s)	1341
TauI	GCSGC	4 cut(s)	696, 975, 1071, 1092
TfiI	GAWTC	5 cut(s)	133, 338, 798, 1291, 1335
Tru1I	TTAA	1 cut(s)	1377
Tru9I	TTAA	1 cut(s)	1377
TseI	GCWGC	5 cut(s)	79, 703, 706, 760, 1002
TspGWI	ACGGA	4 cut(s)	417, 880, 1066, 1257
TspMI	CCCGGG	1 cut(s)	114
Tth111I	GACNNNGTC	3 cut(s)	157, 914, 1265
VpaK11BI	GGWCC	3 cut(s)	60, 346, 460
XmaI	CCCGGG	1 cut(s)	114
XmaJI	CCTAGG	2 cut(s)	55, 599
XmiI	GTMKAC	2 cut(s)	834, 1197
XspI	CTAG	4 cut(s)	56, 600, 614, 1218

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.