Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0000311

Physical Location & Seq

Genomic Coordinates Forward (+)

49672223 .. 49674414

Gene Structure 2192 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G026300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1695 bp

ATGAGGTGTAACACTGTGAAATGCTATTGCCATGCAAAGAGAAGGATGGCTTCAGACTCTTCACAGATCATCTGCTTAGCAGCTCGCCAACAATCTCAGTCAAGCCAGAAGAACGAGTGCCAGCTAGATCAGCTCCAAGCTCGCGAACCTGATAACCGCATTGAGACTGAAGCGGGTAGGATCGAGTCATGGGACTACAACCGCGACGACTTCCAGTGCGCTGGTGTTGCTGTTCAACGAATCACCATTGAAAGAAATGGCCTTCACTTGCCTTCTTACACCCACGCTCCACAACTCATTTACATAGTCCAAGGAAGTGGTTTTCTATCTACTGTATTTCCTGGCTGTCCTGAGACTTTCGAAGAATCTGAACAATCTGATGAAGAACAACAAGGGCAAGGGCAGCAGGGGCGCCATGGACAGCAAGGCCGTGGACAACGGGGTCAACAAGAACAAGAAGAGGAAGAACAACAACAACGCCAACGCCAACAGGGACAACAAGGCCATGGACAGCATGATAGACAACAAGGACAGCAAGGCCAACGGGGACAAGAGAGCTTCGAACAGCTAGACAGACACCAGAAGGTTCGACGCATAAAAGAAGGTGATATTGTTGCTATTCCTGCCGGAGTCACCTACTGGTCCTACAATGACGGTGACCAACCTCTTGTTGCTGTCAGTCTTCTTGACATCAGCAACAACCAAAACCAGCTTGATCGTAACCCTAGGAGATTCTATCTTTCTGGTAACCCACAAGATGAGTTTAAACAACAAGGGCAAAGCCAGAGTCGAAGGGGCCAGCAAGGACGTGGACAAGAAGGGCAAGGCCAAAGTCAATGGGACCAGGACCAGGACCAGCAACAGCAACAGCAAGGAGGAAGGAGCAGGCACCAGCAACAACAGCATGGAAACAACAACAACAACATCTTCGCTGGCTTCAACACACAGCTCCTCGCTGACGCTCTGAACATCGACCAACAGACTGCGCAGCAGCTTCAGGGACAAAACGACAACAGACCCCAAATCGTCAGAGTTAGAGGACGTCTTGATTTTATCCAGCCGCCACAGTCACAACAGGAGGAAAGACAAGAACAGGGAATTCAGAGACAACAGGGACAGAATGGTCTTGAGGAAACTCTCTGCAACTGGAAGATCAGAGAGAATATTGGCAGGCCTTCACGTGCTGACGTCTTCAGTCCCCAAGCCGGTCGCATCAGCACCCTCAACAGCTTTAAGTTGCCTATTCTCAGGTACCTTGACCTCAGCGCTGAGAGAGGCTTCTTCTACAATAATGCTATCTACAGCCCACACTGGAACTTGAACGCCCACGAAGTGTACTACGTCATTCGAGGTAGCGCTAGGGTTCAGGTTGTGAACGACAACGGCCAGGCCATCTTGGACAACGAAGTCCGCCAGGGACAGCTCTTCATTGTGCCACAGAACCACGCAGTGTTACAAAAGGCTAGCAACAACGGCTACGAGTACATTGCCTTCAAGACCCAGGACAATGCCAAGATTAACACTCTGGCAGGCCGGACCTCAGTCTTGCGGGCACTCCCCGACGTCGTCCTTGCTAACGCATACCAGATTGACCGACAACAGGCAAGGAATCTCAAATACAACAGACAGGAGACTGTTGCTTTGAGCTCTTCAAGGTCTTCCCAATCCCAGAGGAGCTGGTGGGCTATTGCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001101 GO:0003006 GO:0003674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0007275 GO:0008150 GO:0009719 GO:0009725 GO:0009735 GO:0009737 GO:0009791 GO:0009987 GO:0010033 GO:0010154 GO:0010431 GO:0021700 GO:0022414 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032504 GO:0032870 GO:0033095 GO:0033993 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045735 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0051716 GO:0061458 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071695 GO:0097305 GO:0097306 GO:0097708 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cupin_1

Protein Analysis

564

Amino Acids

64.51

Weight (kDa)

7.19

Isoelectric Point (pI)

65.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cupin_1	PF00190	46 - 251	5e-25	Cupin
Cupin_1	PF00190	388 - 535	3e-40	Cupin

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000311)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G03880 AT1G03890 AT4G28520 AT4G28520 AT4G28520 AT4G28520 AT4G28520 AT5G44120 AT5G44120 AT5G44120
fragaria_vesca	FvH4_2g21390 FvH4_2g21390 FvH4_2g21400 FvH4_2g21410 FvH4_2g21410 FvH4_2g21410 FvH4_2g21420 FvH4_2g21420 FvH4_2g21420 FvH4_2g21420 FvH4_2g21430 FvH4_2g21430 FvH4_2g21430 FvH4_2g21430 FvH4_2g21430 FvH4_2g21430 FvH4_2g21440 FvH4_3g19080
malus_domestica	MD01G1024700.v1.1 MD05G1196800.v1.1 MD05G1196900.v1.1 MD17G1078200.v1.1
prunus_persica	Prupe.8G223300_v2.0.a1 Prupe.8G223400_v2.0.a1 Prupe.8G223400_v2.0.a1
pyrus_communis	pycom05g18380 pycom05g18390
rosa_chinensis	RchiOBHm_Chr6g0287991 RchiOBHm_Chr6g0288001 RchiOBHm_Chr6g0288021 RchiOBHm_Chr6g0288031 RchiOBHm_Chr6g0288041 RchiOBHm_Chr6g0288051 RchiOBHm_Chr6g0288071
rosa_laevigata	RLG00000012441 RLG00000012443 RLG00000012444 RLG00000012446 RLG00000012447 RLG00000012448
rosa_multiflora	Rmu_co8147412.1_g000001 Rmu_co8487931.1_g000001 Rmu_co8505755.1_g000001 Rmu_sc0001117.1_g000003 Rmu_sc0001117.1_g000005 Rmu_sc0001117.1_g000011 Rmu_sc0001117.1_g000013 Rmu_sc0005266.1_g000010 Rmu_sc0010960.1_g000004
rosa_roxburghii	Rroxscaffold_3G00252330 Rroxscaffold_7G00180530 Rroxscaffold_7G00180550 Rroxscaffold_7G00180560 Rroxscaffold_7G00180570 Rroxscaffold_7G00180580 Rroxscaffold_7G00180590
rosa_rugosa	Rorug06G0041100 Rorug06G0041200 Rorug06G0041200 Rorug06G0041400 Rorug06G0193700 Rorug06G0193700 Rorug06G0193700 Rorug06G0193800 Rorug06G0193800 Rorug06G0193900 Rorug06G0194000 Rorug06G0194100
rosa_samantha	Rh6AG303300 Rh6AG303400 Rh6AG303500 Rh6AG303700 Rh6AG303800 Rh6AG303900 Rh6AG304100 Rh6AG304200 Rh6BG309500 Rh6BG309600 Rh6BG309700 Rh6BG309900 Rh6BG310000 Rh6BG310100 Rh6BG310200 Rh6CG299600 Rh6CG299800 Rh6CG300000 Rh6CG300200 Rh6CG300300 Rh6CG317100 Rh6CG317300 Rh6CG317500 Rh6CG317700 Rh6CG317800 Rh6CG318200 Rh6DG302000 Rh6DG302100 Rh6DG302300 Rh6DG302500 Rh6DG302600 Rh6DG302700 Rh6DG303100
rosa_wichuraiana	Rw6G026230 Rw6G026240 Rw6G026260 Rw6G026280 Rw6G026300 Rw6G026320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	3 cut(s)	441, 1122, 1406
AatII	GACGTC	3 cut(s)	1045, 1191, 1566
Acc16I	TGCGCA	1 cut(s)	987
Acc65I	GGTACC	1 cut(s)	1251
AccB1I	GGYRCC	3 cut(s)	411, 888, 1251
AccII	CGCG	2 cut(s)	144, 204
AciI	CCGC	6 cut(s)	157, 173, 202, 1061, 1411, 1549
AclWI	GGATC	1 cut(s)	188
AcoI	YGGCCR	1 cut(s)	1384
AcsI	RAATTY	1 cut(s)	1098
AcuI	CTGAAG	4 cut(s)	36, 189, 980, 1177
AcvI	CACGTG	1 cut(s)	1181
AcyI	GRCGYC	4 cut(s)	412, 1042, 1188, 1563
AdeI	CACNNNGTG	2 cut(s)	1333, 1450
AfaI	GTAC	3 cut(s)	1253, 1337, 1484
AfeI	AGCGCT	2 cut(s)	1267, 1357
AfiI	CCNNNNNNNGG	3 cut(s)	850, 1205, 1600
AgsI	TTSAA	6 cut(s)	236, 251, 940, 1321, 1495, 1653
AjiI	CACGTC	1 cut(s)	809
AjnI	CCWGG	6 cut(s)	340, 843, 849, 1386, 1413, 1500
Alw21I	GWGCWC	1 cut(s)	1649
Alw26I	GTCTC	4 cut(s)	158, 347, 1099, 1625
AlwI	GGATC	1 cut(s)	188
Aor51HI	AGCGCT	2 cut(s)	1267, 1357
ApeKI	GCWGC	4 cut(s)	80, 403, 988, 991
ApoI	RAATTY	1 cut(s)	1098
ArsI	GACNNNNNNTTYG	4 cut(s)	772, 804, 1624, 1656
Asp718I	GGTACC	1 cut(s)	1251
AspA2I	CCTAGG	1 cut(s)	725
AspLEI	GCGC	5 cut(s)	221, 414, 988, 1268, 1358
AspS9I	GGNCC	6 cut(s)	642, 796, 841, 847, 853, 1536
AsuHPI	GGTGA	4 cut(s)	235, 617, 625, 668
AsuII	TTCGAA	2 cut(s)	360, 561
AsuNHI	GCTAGC	1 cut(s)	1463
AvaII	GGWCC	5 cut(s)	642, 841, 847, 853, 1536
AvrII	CCTAGG	1 cut(s)	725
BaeGI	GKGCMC	1 cut(s)	1555
BanI	GGYRCC	3 cut(s)	411, 888, 1251
BanII	GRGCYC	1 cut(s)	1649
BarI	GAAGNNNNNNTAC	2 cut(s)	1475, 1507
BbrPI	CACGTG	1 cut(s)	1181
BbsI	GAAGAC	3 cut(s)	674, 1183, 1650
Bbv12I	GWGCWC	1 cut(s)	1649
BbvCI	CCTCAGC	1 cut(s)	1262
BbvI	GCAGC	4 cut(s)	92, 415, 1000, 1003
BccI	CCATC	2 cut(s)	40, 1400
BceAI	ACGGC	3 cut(s)	414, 1399, 1489
BcgI	CGANNNNNNTGC	2 cut(s)	1469, 1503
BciT130I	CCWGG	6 cut(s)	342, 845, 851, 1388, 1415, 1502
BcoDI	GTCTC	4 cut(s)	158, 347, 1099, 1625
BfaI	CTAG	5 cut(s)	125, 569, 726, 1359, 1464
BfmI	CTRYAG	1 cut(s)	1300
BfoI	RGCGCY	3 cut(s)	415, 1269, 1359
BisI	GCNGC	5 cut(s)	81, 404, 989, 992, 1061
BlnI	CCTAGG	1 cut(s)	725
BlpI	GCTNAGC	1 cut(s)	76
BlsI	GCNGC	5 cut(s)	82, 405, 990, 993, 1062
Bme1390I	CCNGG	6 cut(s)	342, 845, 851, 1388, 1415, 1502
Bme18I	GGWCC	5 cut(s)	642, 841, 847, 853, 1536
BmgBI	CACGTC	1 cut(s)	809
BmgT120I	GGNCC	6 cut(s)	642, 796, 841, 847, 853, 1536
BmiI	GGNNCC	5 cut(s)	413, 797, 842, 890, 1253
BmrFI	CCNGG	6 cut(s)	342, 845, 851, 1388, 1415, 1502
BmsI	GCATC	1 cut(s)	1221
BmtI	GCTAGC	1 cut(s)	1467
BoxI	GACNNNNGTC	1 cut(s)	1541
BpiI	GAAGAC	3 cut(s)	674, 1183, 1650
Bpu10I	CCTNAGC	1 cut(s)	1262
Bpu1102I	GCTNAGC	1 cut(s)	76
Bpu14I	TTCGAA	2 cut(s)	360, 561
BpuEI	CTTGAG	1 cut(s)	1148
BsaAI	YACGTR	1 cut(s)	1181
BsaHI	GRCGYC	4 cut(s)	412, 1042, 1188, 1563
BsaJI	CCNNGG	7 cut(s)	310, 415, 430, 505, 725, 1414, 1500
BsaXI	ACNNNNNCTCC	2 cut(s)	271, 301
Bsc4I	CCNNNNNNNGG	3 cut(s)	850, 1205, 1600
Bse118I	RCCGGY	1 cut(s)	1205
Bse1I	ACTGG	4 cut(s)	214, 644, 1151, 1316
Bse3DI	GCAATG	1 cut(s)	1485
BseBI	CCWGG	6 cut(s)	342, 845, 851, 1388, 1415, 1502
BseDI	CCNNGG	7 cut(s)	310, 415, 430, 505, 725, 1414, 1500
BseGI	GGATG	1 cut(s)	51
BseLI	CCNNNNNNNGG	3 cut(s)	850, 1205, 1600
BseMI	GCAATG	1 cut(s)	1485
BseMII	CTCAG	6 cut(s)	110, 342, 1260, 1261, 1276, 1554
BseNI	ACTGG	4 cut(s)	214, 644, 1151, 1316
BseRI	GAGGAG	2 cut(s)	941, 1687
BseSI	GKGCMC	1 cut(s)	1555
BseXI	GCAGC	4 cut(s)	92, 415, 1000, 1003
Bsh1236I	CGCG	2 cut(s)	144, 204
Bsh1285I	CGRYCG	1 cut(s)	1210
BshNI	GGYRCC	3 cut(s)	411, 888, 1251
BsiEI	CGRYCG	1 cut(s)	1210
BsiHKAI	GWGCWC	1 cut(s)	1649
BsiSI	CCGG	3 cut(s)	627, 1206, 1534
BslFI	GGGAC	8 cut(s)	206, 507, 561, 854, 1014, 1128, 1182, 1431
BslI	CCNNNNNNNGG	3 cut(s)	850, 1205, 1600
BsmAI	GTCTC	4 cut(s)	158, 347, 1099, 1625
BsmFI	GGGAC	8 cut(s)	206, 507, 561, 854, 1014, 1128, 1182, 1431
Bsp119I	TTCGAA	2 cut(s)	360, 561
Bsp1286I	GDGCHC	2 cut(s)	1555, 1649
Bsp143I	GATC	5 cut(s)	66, 127, 180, 715, 1152
Bsp1720I	GCTNAGC	1 cut(s)	76
Bsp19I	CCATGG	2 cut(s)	415, 505
Bsp68I	TCGCGA	1 cut(s)	144
BspACI	CCGC	6 cut(s)	157, 173, 202, 1061, 1411, 1549
BspCNI	CTCAG	6 cut(s)	109, 343, 1260, 1261, 1275, 1553
BspFNI	CGCG	2 cut(s)	144, 204
BspLI	GGNNCC	5 cut(s)	413, 797, 842, 890, 1253
BspOI	GCTAGC	1 cut(s)	1467
BspPI	GGATC	1 cut(s)	188
BspQI	GCTCTTC	2 cut(s)	1430, 1654
BspT104I	TTCGAA	2 cut(s)	360, 561
BspT107I	GGYRCC	3 cut(s)	411, 888, 1251
BsrDI	GCAATG	1 cut(s)	1485
BsrFI	RCCGGY	1 cut(s)	1205
BsrI	ACTGG	4 cut(s)	214, 644, 1151, 1316
BssAI	RCCGGY	1 cut(s)	1205
BssECI	CCNNGG	7 cut(s)	310, 415, 430, 505, 725, 1414, 1500
BssMI	GATC	5 cut(s)	66, 127, 180, 715, 1152
BssNI	GRCGYC	4 cut(s)	412, 1042, 1188, 1563
BssT1I	CCWWGG	4 cut(s)	310, 415, 505, 725
Bst2UI	CCWGG	6 cut(s)	342, 845, 851, 1388, 1415, 1502
Bst4CI	ACNGT	5 cut(s)	16, 334, 656, 1068, 1636
Bst6I	CTCTTC	4 cut(s)	64, 453, 1430, 1654
BstACI	GRCGYC	4 cut(s)	412, 1042, 1188, 1563
BstBAI	YACGTR	1 cut(s)	1181
BstBI	TTCGAA	2 cut(s)	360, 561
BstDEI	CTNAG	7 cut(s)	76, 96, 351, 1247, 1262, 1269, 1540
BstDSI	CCRYGG	3 cut(s)	415, 430, 505
BstEII	GGTNACC	2 cut(s)	656, 746
BstF5I	GGATG	1 cut(s)	51
BstFNI	CGCG	2 cut(s)	144, 204
BstH2I	RGCGCY	3 cut(s)	415, 1269, 1359
BstHHI	GCGC	5 cut(s)	221, 414, 988, 1268, 1358
BstKTI	GATC	5 cut(s)	69, 130, 183, 718, 1155
BstMAI	GTCTC	4 cut(s)	158, 347, 1099, 1625
BstMBI	GATC	5 cut(s)	66, 127, 180, 715, 1152
BstMCI	CGRYCG	1 cut(s)	1210
BstMWI	GCNNNNNNNGC	7 cut(s)	130, 227, 403, 409, 623, 901, 1473
BstNI	CCWGG	6 cut(s)	342, 845, 851, 1388, 1415, 1502
BstPAI	GACNNNNGTC	1 cut(s)	1541
BstPI	GGTNACC	2 cut(s)	656, 746
BstSCI	CCNGG	6 cut(s)	340, 843, 849, 1386, 1413, 1500
BstSFI	CTRYAG	1 cut(s)	1300
BstSLI	GKGCMC	1 cut(s)	1555
BstUI	CGCG	2 cut(s)	144, 204
BstV1I	GCAGC	4 cut(s)	92, 415, 1000, 1003
BstV2I	GAAGAC	3 cut(s)	674, 1183, 1650
BstXI	CCANNNNNNTGG	2 cut(s)	221, 317
BtgI	CCRYGG	3 cut(s)	415, 430, 505
BtrI	CACGTC	1 cut(s)	809
BtsCI	GGATG	1 cut(s)	51
BtsI	GCAGTG	1 cut(s)	1455
BtsIMutI	CAGTG	4 cut(s)	12, 221, 1309, 1455
BtuMI	TCGCGA	1 cut(s)	144
CfoI	GCGC	5 cut(s)	221, 414, 988, 1268, 1358
Cfr10I	RCCGGY	1 cut(s)	1205
Cfr13I	GGNCC	6 cut(s)	642, 796, 841, 847, 853, 1536
CseI	GACGC	2 cut(s)	600, 968
Csp6I	GTAC	3 cut(s)	1252, 1336, 1483
CviAII	CATG	6 cut(s)	32, 189, 416, 506, 515, 905
CviQI	GTAC	3 cut(s)	1252, 1336, 1483
DdeI	CTNAG	7 cut(s)	76, 96, 351, 1247, 1262, 1269, 1540
DinI	GGCGCC	1 cut(s)	413
DpnI	GATC	5 cut(s)	68, 129, 182, 717, 1154
DpnII	GATC	5 cut(s)	66, 127, 180, 715, 1152
DraI	TTTAAA	1 cut(s)	766
DraIII	CACNNNGTG	2 cut(s)	1333, 1450
DrdI	GACNNNNNNGTC	3 cut(s)	441, 1122, 1406
DseDI	GACNNNNNNGTC	3 cut(s)	441, 1122, 1406
EaeI	YGGCCR	1 cut(s)	1384
Eam1104I	CTCTTC	4 cut(s)	64, 453, 1430, 1654
EarI	CTCTTC	4 cut(s)	64, 453, 1430, 1654
EciI	GGCGGA	1 cut(s)	1400
Ecl136II	GAGCTC	1 cut(s)	1647
Eco130I	CCWWGG	4 cut(s)	310, 415, 505, 725
Eco147I	AGGCCT	1 cut(s)	1174
Eco24I	GRGCYC	1 cut(s)	1649
Eco47I	GGWCC	5 cut(s)	642, 841, 847, 853, 1536
Eco47III	AGCGCT	2 cut(s)	1267, 1357
Eco53kI	GAGCTC	1 cut(s)	1647
Eco57I	CTGAAG	4 cut(s)	36, 189, 980, 1177
Eco72I	CACGTG	1 cut(s)	1181
Eco91I	GGTNACC	2 cut(s)	656, 746
EcoICRI	GAGCTC	1 cut(s)	1647
EcoO65I	GGTNACC	2 cut(s)	656, 746
EcoRI	GAATTC	1 cut(s)	1098
EcoRII	CCWGG	6 cut(s)	340, 843, 849, 1386, 1413, 1500
EcoT14I	CCWWGG	4 cut(s)	310, 415, 505, 725
EcoT38I	GRGCYC	1 cut(s)	1649
EgeI	GGCGCC	1 cut(s)	413
EheI	GGCGCC	1 cut(s)	413
ErhI	CCWWGG	4 cut(s)	310, 415, 505, 725
FaeI	CATG	6 cut(s)	35, 192, 419, 509, 518, 908
FalI	AAGNNNNNCTT	2 cut(s)	34, 66
FaqI	GGGAC	8 cut(s)	206, 507, 561, 854, 1014, 1128, 1182, 1431
FatI	CATG	6 cut(s)	31, 188, 415, 505, 514, 904
FauI	CCCGC	2 cut(s)	166, 1542
Fnu4HI	GCNGC	5 cut(s)	81, 404, 989, 992, 1061
FokI	GGATG	1 cut(s)	58
FriOI	GRGCYC	1 cut(s)	1649
Fsp4HI	GCNGC	5 cut(s)	81, 404, 989, 992, 1061
FspBI	CTAG	5 cut(s)	125, 569, 726, 1359, 1464
FspI	TGCGCA	1 cut(s)	987
GlaI	GCGC	5 cut(s)	220, 413, 987, 1267, 1357
GluI	GCNGC	5 cut(s)	81, 404, 989, 992, 1061
HaeII	RGCGCY	3 cut(s)	415, 1269, 1359
HapII	CCGG	3 cut(s)	627, 1206, 1534
HgaI	GACGC	2 cut(s)	600, 968
HhaI	GCGC	5 cut(s)	221, 414, 988, 1268, 1358
Hin1I	GRCGYC	4 cut(s)	412, 1042, 1188, 1563
Hin1II	CATG	6 cut(s)	35, 192, 419, 509, 518, 908
Hin6I	GCGC	5 cut(s)	219, 412, 986, 1266, 1356
HinP1I	GCGC	5 cut(s)	219, 412, 986, 1266, 1356
HincII	GTYRAC	1 cut(s)	446
HindII	GTYRAC	1 cut(s)	446
HinfI	GANTC	8 cut(s)	56, 185, 240, 365, 630, 732, 787, 1609
HpaII	CCGG	3 cut(s)	627, 1206, 1534
HphI	GGTGA	4 cut(s)	235, 617, 625, 668
Hpy166II	GTNNAC	5 cut(s)	434, 446, 812, 1336, 1375
Hpy188I	TCNGA	7 cut(s)	55, 370, 379, 966, 1031, 1104, 1157
Hpy188III	TCNNGA	6 cut(s)	143, 350, 686, 1046, 1127, 1495
Hpy8I	GTNNAC	5 cut(s)	434, 446, 812, 1336, 1375
Hpy99I	CGWCG	4 cut(s)	209, 594, 1565, 1568
HpyCH4III	ACNGT	5 cut(s)	16, 334, 656, 1068, 1636
HpyCH4IV	ACGT	6 cut(s)	808, 1042, 1180, 1188, 1341, 1563
HpyCH4V	TGCA	3 cut(s)	35, 1143, 1691
HpyF10VI	GCNNNNNNNGC	7 cut(s)	130, 227, 403, 409, 623, 901, 1473
HpyF3I	CTNAG	7 cut(s)	76, 96, 351, 1247, 1262, 1269, 1540
HpySE526I	ACGT	6 cut(s)	808, 1042, 1180, 1188, 1341, 1563
Hsp92I	GRCGYC	4 cut(s)	412, 1042, 1188, 1563
Hsp92II	CATG	6 cut(s)	35, 192, 419, 509, 518, 908
HspAI	GCGC	5 cut(s)	219, 412, 986, 1266, 1356
KasI	GGCGCC	1 cut(s)	411
KpnI	GGTACC	1 cut(s)	1255
Kzo9I	GATC	5 cut(s)	66, 127, 180, 715, 1152
LguI	GCTCTTC	2 cut(s)	1430, 1654
LmnI	GCTCC	5 cut(s)	138, 292, 882, 954, 1674
Lsp1109I	GCAGC	4 cut(s)	92, 415, 1000, 1003
LweI	GCATC	1 cut(s)	1221
MaeI	CTAG	5 cut(s)	125, 569, 726, 1359, 1464
MaeII	ACGT	6 cut(s)	808, 1042, 1180, 1188, 1341, 1563
MaeIII	GTNAC	7 cut(s)	8, 631, 656, 719, 746, 1068, 1452
MalI	GATC	5 cut(s)	68, 129, 182, 717, 1154
MboI	GATC	5 cut(s)	66, 127, 180, 715, 1152
MhlI	GDGCHC	2 cut(s)	1555, 1649
MluCI	AATT	1 cut(s)	1098
Mly113I	GGCGCC	1 cut(s)	412
MlyI	GAGTC	4 cut(s)	50, 194, 639, 796
MseI	TTAA	3 cut(s)	765, 1233, 1518
MspI	CCGG	3 cut(s)	627, 1206, 1534
MspR9I	CCNGG	6 cut(s)	342, 845, 851, 1388, 1415, 1502
MssI	GTTTAAAC	1 cut(s)	766
MvaI	CCWGG	6 cut(s)	342, 845, 851, 1388, 1415, 1502
MvnI	CGCG	2 cut(s)	144, 204
MwoI	GCNNNNNNNGC	7 cut(s)	130, 227, 403, 409, 623, 901, 1473
NarI	GGCGCC	1 cut(s)	412
NcoI	CCATGG	2 cut(s)	415, 505
NdeII	GATC	5 cut(s)	66, 127, 180, 715, 1152
NheI	GCTAGC	1 cut(s)	1463
NlaIII	CATG	6 cut(s)	35, 192, 419, 509, 518, 908
NlaIV	GGNNCC	5 cut(s)	413, 797, 842, 890, 1253
NmuCI	GTSAC	3 cut(s)	631, 656, 1068
NruI	TCGCGA	1 cut(s)	144
NsbI	TGCGCA	1 cut(s)	987
NspV	TTCGAA	2 cut(s)	360, 561
PceI	AGGCCT	1 cut(s)	1174
PciSI	GCTCTTC	2 cut(s)	1430, 1654
PfeI	GAWTC	4 cut(s)	240, 365, 732, 1609
PflFI	GACNNNGTC	1 cut(s)	1565
PkrI	GCNGC	5 cut(s)	82, 405, 990, 993, 1062
PleI	GAGTC	4 cut(s)	50, 193, 638, 795
PluTI	GGCGCC	1 cut(s)	415
PmaCI	CACGTG	1 cut(s)	1181
PmeI	GTTTAAAC	1 cut(s)	766
PmlI	CACGTG	1 cut(s)	1181
PpsI	GAGTC	4 cut(s)	50, 193, 638, 795
Ppu21I	YACGTR	1 cut(s)	1181
PshAI	GACNNNNGTC	1 cut(s)	1541
Psp124BI	GAGCTC	1 cut(s)	1649
Psp6I	CCWGG	6 cut(s)	340, 843, 849, 1386, 1413, 1500
PspCI	CACGTG	1 cut(s)	1181
PspEI	GGTNACC	2 cut(s)	656, 746
PspGI	CCWGG	6 cut(s)	340, 843, 849, 1386, 1413, 1500
PspN4I	GGNNCC	5 cut(s)	413, 797, 842, 890, 1253
PspPI	GGNCC	6 cut(s)	642, 796, 841, 847, 853, 1536
PsyI	GACNNNGTC	1 cut(s)	1565
RruI	TCGCGA	1 cut(s)	144
RsaI	GTAC	3 cut(s)	1253, 1337, 1484
RsaNI	GTAC	3 cut(s)	1252, 1336, 1483
SacI	GAGCTC	1 cut(s)	1649
SapI	GCTCTTC	2 cut(s)	1430, 1654
SaqAI	TTAA	3 cut(s)	765, 1233, 1518
SatI	GCNGC	5 cut(s)	81, 404, 989, 992, 1061
Sau3AI	GATC	5 cut(s)	66, 127, 180, 715, 1152
Sau96I	GGNCC	6 cut(s)	642, 796, 841, 847, 853, 1536
SchI	GAGTC	4 cut(s)	50, 194, 639, 796
ScrFI	CCNGG	6 cut(s)	342, 845, 851, 1388, 1415, 1502
SduI	GDGCHC	2 cut(s)	1555, 1649
SfaNI	GCATC	1 cut(s)	1221
SfcI	CTRYAG	1 cut(s)	1300
SfoI	GGCGCC	1 cut(s)	413
SfuI	TTCGAA	2 cut(s)	360, 561
SinI	GGWCC	5 cut(s)	642, 841, 847, 853, 1536
SmlI	CTYRAG	1 cut(s)	1127
SmoI	CTYRAG	1 cut(s)	1127
Sse9I	AATT	1 cut(s)	1098
SseBI	AGGCCT	1 cut(s)	1174
SsiI	CCGC	6 cut(s)	157, 173, 202, 1061, 1411, 1549
SspDI	GGCGCC	1 cut(s)	411
SspI	AATATT	1 cut(s)	1165
SspMI	CTAG	5 cut(s)	125, 569, 726, 1359, 1464
SstI	GAGCTC	1 cut(s)	1649
StuI	AGGCCT	1 cut(s)	1174
StyD4I	CCNGG	6 cut(s)	340, 843, 849, 1386, 1413, 1500
StyI	CCWWGG	4 cut(s)	310, 415, 505, 725
TaaI	ACNGT	5 cut(s)	16, 334, 656, 1068, 1636
TaiI	ACGT	6 cut(s)	811, 1045, 1183, 1191, 1344, 1566
TaqI	TCGA	7 cut(s)	183, 360, 561, 589, 790, 972, 1348
TaqII	GACCGA	1 cut(s)	1608
TasI	AATT	1 cut(s)	1098
TatI	WGTACW	2 cut(s)	1335, 1482
TauI	GCSGC	1 cut(s)	1063
TfiI	GAWTC	4 cut(s)	240, 365, 732, 1609
Tru1I	TTAA	3 cut(s)	765, 1233, 1518
Tru9I	TTAA	3 cut(s)	765, 1233, 1518
TscAI	CASTG	4 cut(s)	19, 221, 1316, 1455
TseFI	GTSAC	3 cut(s)	631, 656, 1068
TseI	GCWGC	4 cut(s)	80, 403, 988, 991
Tsp45I	GTSAC	3 cut(s)	631, 656, 1068
TspDTI	ATGAA	2 cut(s)	396, 1417
TspRI	CASTG	4 cut(s)	19, 221, 1316, 1455
Tth111I	GACNNNGTC	1 cut(s)	1565
VpaK11BI	GGWCC	5 cut(s)	642, 841, 847, 853, 1536
XapI	RAATTY	1 cut(s)	1098
XcmI	CCANNNNNNNNNTGG	1 cut(s)	806
XmaJI	CCTAGG	1 cut(s)	725
XspI	CTAG	5 cut(s)	125, 569, 726, 1359, 1464
ZraI	GACGTC	3 cut(s)	1043, 1189, 1564

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.