Seed storage protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1745 |
| Acc36I | ACCTGC | 1 cut(s) | 1745 |
| AccB1I | GGYRCC | 1 cut(s) | 413 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1703 |
| AccII | CGCG | 1 cut(s) | 1277 |
| AciI | CCGC | 1 cut(s) | 855 |
| AclWI | GGATC | 3 cut(s) | 1407, 1450, 1493 |
| AcoI | YGGCCR | 2 cut(s) | 822, 852 |
| AcsI | RAATTY | 6 cut(s) | 325, 699, 918, 1010, 1069, 1456 |
| AcuI | CTGAAG | 1 cut(s) | 531 |
| AdeI | CACNNNGTG | 1 cut(s) | 305 |
| AfaI | GTAC | 8 cut(s) | 93, 255, 628, 973, 1088, 1136, 1343, 1375 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 835, 1535, 1703 |
| AgsI | TTSAA | 7 cut(s) | 65, 314, 324, 618, 906, 1358, 1558 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1276 |
| AjnI | CCWGG | 6 cut(s) | 13, 316, 595, 793, 1032, 1570 |
| AleI | CACNNNNGTG | 1 cut(s) | 370 |
| AluBI | AGCT | 5 cut(s) | 1097, 1151, 1173, 1428, 1626 |
| AluI | AGCT | 5 cut(s) | 1097, 1151, 1173, 1428, 1626 |
| Alw26I | GTCTC | 3 cut(s) | 837, 1028, 1655 |
| AlwI | GGATC | 3 cut(s) | 1407, 1450, 1493 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1451 |
| Ama87I | CYCGRG | 1 cut(s) | 923 |
| AoxI | GGCC | 3 cut(s) | 164, 822, 852 |
| ApeKI | GCWGC | 3 cut(s) | 49, 1001, 1151 |
| ApoI | RAATTY | 6 cut(s) | 325, 699, 918, 1010, 1069, 1456 |
| AseI | ATTAAT | 1 cut(s) | 678 |
| AspS9I | GGNCC | 5 cut(s) | 164, 284, 393, 538, 791 |
| AsuC2I | CCSGG | 1 cut(s) | 162 |
| AsuHPI | GGTGA | 1 cut(s) | 1701 |
| AvaI | CYCGRG | 1 cut(s) | 923 |
| AvaII | GGWCC | 4 cut(s) | 284, 393, 538, 791 |
| BaeGI | GKGCMC | 1 cut(s) | 842 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1365, 1398 |
| BanI | GGYRCC | 1 cut(s) | 413 |
| BbvCI | CCTCAGC | 1 cut(s) | 534 |
| BbvI | GCAGC | 3 cut(s) | 36, 1013, 1138 |
| BccI | CCATC | 8 cut(s) | 275, 461, 542, 1192, 1267, 1533, 1640, 1724 |
| BceAI | ACGGC | 1 cut(s) | 837 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 512, 546 |
| BciT130I | CCWGG | 6 cut(s) | 15, 318, 597, 795, 1034, 1572 |
| BciVI | GTATCC | 1 cut(s) | 184 |
| BclI | TGATCA | 1 cut(s) | 816 |
| BcnI | CCSGG | 1 cut(s) | 162 |
| BcoDI | GTCTC | 3 cut(s) | 837, 1028, 1655 |
| BfaI | CTAG | 3 cut(s) | 624, 858, 1155 |
| BfmI | CTRYAG | 2 cut(s) | 1210, 1423 |
| BfuAI | ACCTGC | 1 cut(s) | 1745 |
| BfuI | GTATCC | 1 cut(s) | 184 |
| BisI | GCNGC | 4 cut(s) | 50, 855, 1002, 1152 |
| BlpI | GCTNAGC | 1 cut(s) | 1742 |
| BlsI | GCNGC | 4 cut(s) | 51, 856, 1003, 1153 |
| Bme1390I | CCNGG | 7 cut(s) | 15, 162, 318, 597, 795, 1034, 1572 |
| Bme18I | GGWCC | 4 cut(s) | 284, 393, 538, 791 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1276 |
| BmeT110I | CYCGRG | 1 cut(s) | 923 |
| BmgT120I | GGNCC | 5 cut(s) | 164, 284, 393, 538, 791 |
| BmiI | GGNNCC | 3 cut(s) | 165, 415, 1321 |
| BmrFI | CCNGG | 7 cut(s) | 15, 162, 318, 597, 795, 1034, 1572 |
| BmsI | GCATC | 4 cut(s) | 36, 736, 758, 1378 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 591, 623 |
| Bpu10I | CCTNAGC | 1 cut(s) | 534 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1742 |
| BpuEI | CTTGAG | 2 cut(s) | 80, 1010 |
| BpuMI | CCSGG | 1 cut(s) | 162 |
| BsaAI | YACGTR | 3 cut(s) | 630, 1202, 1673 |
| BsaJI | CCNNGG | 6 cut(s) | 161, 272, 409, 1323, 1369, 1571 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 835, 1535, 1703 |
| Bse1I | ACTGG | 4 cut(s) | 167, 248, 829, 1147 |
| Bse3DI | GCAATG | 1 cut(s) | 52 |
| BseBI | CCWGG | 6 cut(s) | 15, 318, 597, 795, 1034, 1572 |
| BseDI | CCNNGG | 6 cut(s) | 161, 272, 409, 1323, 1369, 1571 |
| BseGI | GGATG | 4 cut(s) | 51, 751, 1402, 1544 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 835, 1535, 1703 |
| BseMI | GCAATG | 1 cut(s) | 52 |
| BseMII | CTCAG | 6 cut(s) | 279, 525, 848, 1251, 1386, 1650 |
| BseNI | ACTGG | 4 cut(s) | 167, 248, 829, 1147 |
| BseSI | GKGCMC | 1 cut(s) | 842 |
| BseXI | GCAGC | 3 cut(s) | 36, 1013, 1138 |
| BseYI | CCCAGC | 2 cut(s) | 1051, 1173 |
| Bsh1236I | CGCG | 1 cut(s) | 1277 |
| BshFI | GGCC | 3 cut(s) | 166, 824, 854 |
| BshNI | GGYRCC | 1 cut(s) | 413 |
| BsiHKCI | CYCGRG | 1 cut(s) | 923 |
| BsiSI | CCGG | 1 cut(s) | 161 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 835, 1535, 1703 |
| BsmAI | GTCTC | 3 cut(s) | 837, 1028, 1655 |
| BsmBI | CGTCTC | 2 cut(s) | 1028, 1655 |
| BsmI | GAATGC | 1 cut(s) | 71 |
| BsnI | GGCC | 3 cut(s) | 166, 824, 854 |
| BsoBI | CYCGRG | 1 cut(s) | 923 |
| Bsp1286I | GDGCHC | 1 cut(s) | 842 |
| Bsp143I | GATC | 7 cut(s) | 816, 1018, 1302, 1399, 1442, 1498, 1600 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1742 |
| Bsp19I | CCATGG | 2 cut(s) | 272, 409 |
| BspACI | CCGC | 1 cut(s) | 855 |
| BspANI | GGCC | 3 cut(s) | 166, 824, 854 |
| BspCNI | CTCAG | 6 cut(s) | 280, 526, 847, 1250, 1387, 1651 |
| BspFNI | CGCG | 1 cut(s) | 1277 |
| BspHI | TCATGA | 1 cut(s) | 783 |
| BspLI | GGNNCC | 3 cut(s) | 165, 415, 1321 |
| BspMI | ACCTGC | 1 cut(s) | 1745 |
| BspPI | GGATC | 3 cut(s) | 1407, 1450, 1493 |
| BspT107I | GGYRCC | 1 cut(s) | 413 |
| BsrDI | GCAATG | 1 cut(s) | 52 |
| BsrI | ACTGG | 4 cut(s) | 167, 248, 829, 1147 |
| BssECI | CCNNGG | 6 cut(s) | 161, 272, 409, 1323, 1369, 1571 |
| BssMI | GATC | 7 cut(s) | 816, 1018, 1302, 1399, 1442, 1498, 1600 |
| BssT1I | CCWWGG | 4 cut(s) | 272, 409, 1323, 1369 |
| Bst2UI | CCWGG | 6 cut(s) | 15, 318, 597, 795, 1034, 1572 |
| Bst4CI | ACNGT | 5 cut(s) | 196, 733, 874, 910, 1519 |
| Bst6I | CTCTTC | 1 cut(s) | 1344 |
| BstBAI | YACGTR | 3 cut(s) | 630, 1202, 1673 |
| BstC8I | GCNNGC | 2 cut(s) | 137, 378 |
| BstDEI | CTNAG | 7 cut(s) | 288, 534, 834, 1237, 1395, 1659, 1742 |
| BstDSI | CCRYGG | 2 cut(s) | 272, 409 |
| BstF5I | GGATG | 4 cut(s) | 51, 751, 1402, 1544 |
| BstFNI | CGCG | 1 cut(s) | 1277 |
| BstKTI | GATC | 7 cut(s) | 819, 1021, 1305, 1402, 1445, 1501, 1603 |
| BstMAI | GTCTC | 3 cut(s) | 837, 1028, 1655 |
| BstMBI | GATC | 7 cut(s) | 816, 1018, 1302, 1399, 1442, 1498, 1600 |
| BstNI | CCWGG | 6 cut(s) | 15, 318, 597, 795, 1034, 1572 |
| BstNSI | RCATGY | 4 cut(s) | 139, 380, 1417, 1693 |
| BstSCI | CCNGG | 7 cut(s) | 13, 160, 316, 595, 793, 1032, 1570 |
| BstSFI | CTRYAG | 2 cut(s) | 1210, 1423 |
| BstSLI | GKGCMC | 1 cut(s) | 842 |
| BstSNI | TACGTA | 2 cut(s) | 630, 1673 |
| BstUI | CGCG | 1 cut(s) | 1277 |
| BstV1I | GCAGC | 3 cut(s) | 36, 1013, 1138 |
| BsuI | GTATCC | 1 cut(s) | 184 |
| BsuRI | GGCC | 3 cut(s) | 166, 824, 854 |
| BtgI | CCRYGG | 2 cut(s) | 272, 409 |
| BtsCI | GGATG | 4 cut(s) | 51, 751, 1402, 1544 |
| BtsI | GCAGTG | 1 cut(s) | 559 |
| BtsIMutI | CAGTG | 4 cut(s) | 241, 559, 729, 870 |
| BveI | ACCTGC | 1 cut(s) | 1745 |
| Cac8I | GCNNGC | 2 cut(s) | 137, 378 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1451 |
| CciI | TCATGA | 1 cut(s) | 783 |
| Cfr13I | GGNCC | 5 cut(s) | 164, 284, 393, 538, 791 |
| CseI | GACGC | 1 cut(s) | 1266 |
| CsiI | ACCWGGT | 1 cut(s) | 316 |
| Csp6I | GTAC | 8 cut(s) | 92, 254, 627, 972, 1087, 1135, 1342, 1374 |
| CviQI | GTAC | 8 cut(s) | 92, 254, 627, 972, 1087, 1135, 1342, 1374 |
| DdeI | CTNAG | 7 cut(s) | 288, 534, 834, 1237, 1395, 1659, 1742 |
| DpnI | GATC | 7 cut(s) | 818, 1020, 1304, 1401, 1444, 1500, 1602 |
| DpnII | GATC | 7 cut(s) | 816, 1018, 1302, 1399, 1442, 1498, 1600 |
| DraI | TTTAAA | 1 cut(s) | 1161 |
| DraIII | CACNNNGTG | 1 cut(s) | 305 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1276 |
| EaeI | YGGCCR | 2 cut(s) | 822, 852 |
| Eam1104I | CTCTTC | 1 cut(s) | 1344 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1276 |
| EarI | CTCTTC | 1 cut(s) | 1344 |
| Eco105I | TACGTA | 2 cut(s) | 630, 1673 |
| Eco130I | CCWWGG | 4 cut(s) | 272, 409, 1323, 1369 |
| Eco47I | GGWCC | 4 cut(s) | 284, 393, 538, 791 |
| Eco57I | CTGAAG | 1 cut(s) | 531 |
| Eco88I | CYCGRG | 1 cut(s) | 923 |
| EcoO109I | RGGNCCY | 1 cut(s) | 538 |
| EcoRI | GAATTC | 1 cut(s) | 918 |
| EcoRII | CCWGG | 6 cut(s) | 13, 316, 595, 793, 1032, 1570 |
| EcoT14I | CCWWGG | 4 cut(s) | 272, 409, 1323, 1369 |
| EcoT22I | ATGCAT | 2 cut(s) | 751, 1419 |
| ErhI | CCWWGG | 4 cut(s) | 272, 409, 1323, 1369 |
| Esp3I | CGTCTC | 2 cut(s) | 1028, 1655 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 92, 124, 351, 383 |
| FauNDI | CATATG | 1 cut(s) | 754 |
| FbaI | TGATCA | 1 cut(s) | 816 |
| Fnu4HI | GCNGC | 4 cut(s) | 50, 855, 1002, 1152 |
| FokI | GGATG | 4 cut(s) | 58, 758, 1389, 1551 |
| Fsp4HI | GCNGC | 4 cut(s) | 50, 855, 1002, 1152 |
| FspBI | CTAG | 3 cut(s) | 624, 858, 1155 |
| GluI | GCNGC | 4 cut(s) | 50, 855, 1002, 1152 |
| GsaI | CCCAGC | 2 cut(s) | 1055, 1177 |
| HaeIII | GGCC | 3 cut(s) | 166, 824, 854 |
| HapII | CCGG | 1 cut(s) | 161 |
| HgaI | GACGC | 1 cut(s) | 1266 |
| HinfI | GANTC | 2 cut(s) | 231, 1554 |
| HpaII | CCGG | 1 cut(s) | 161 |
| HphI | GGTGA | 1 cut(s) | 1701 |
| Hpy166II | GTNNAC | 4 cut(s) | 211, 520, 1676, 1709 |
| Hpy188I | TCNGA | 6 cut(s) | 280, 352, 571, 1240, 1282, 1396 |
| Hpy188III | TCNNGA | 6 cut(s) | 97, 287, 784, 923, 1027, 1292 |
| Hpy8I | GTNNAC | 4 cut(s) | 211, 520, 1676, 1709 |
| HpyAV | CCTTC | 4 cut(s) | 94, 353, 874, 1217 |
| HpyCH4III | ACNGT | 5 cut(s) | 196, 733, 874, 910, 1519 |
| HpyCH4IV | ACGT | 3 cut(s) | 629, 1201, 1672 |
| HpyF3I | CTNAG | 7 cut(s) | 288, 534, 834, 1237, 1395, 1659, 1742 |
| HpySE526I | ACGT | 3 cut(s) | 629, 1201, 1672 |
| Ksp22I | TGATCA | 1 cut(s) | 816 |
| Kzo9I | GATC | 7 cut(s) | 816, 1018, 1302, 1399, 1442, 1498, 1600 |
| Lsp1109I | GCAGC | 3 cut(s) | 36, 1013, 1138 |
| LweI | GCATC | 4 cut(s) | 36, 736, 758, 1378 |
| MabI | ACCWGGT | 1 cut(s) | 316 |
| MaeI | CTAG | 3 cut(s) | 624, 858, 1155 |
| MaeII | ACGT | 3 cut(s) | 629, 1201, 1672 |
| MaeIII | GTNAC | 6 cut(s) | 122, 232, 554, 769, 910, 1197 |
| MalI | GATC | 7 cut(s) | 818, 1020, 1304, 1401, 1444, 1500, 1602 |
| MboI | GATC | 7 cut(s) | 816, 1018, 1302, 1399, 1442, 1498, 1600 |
| MboII | GAAGA | 7 cut(s) | 485, 524, 656, 659, 1078, 1295, 1361 |
| MfeI | CAATTG | 1 cut(s) | 711 |
| MhlI | GDGCHC | 1 cut(s) | 842 |
| MlyI | GAGTC | 1 cut(s) | 240 |
| MmeI | TCCRAC | 1 cut(s) | 1420 |
| Mph1103I | ATGCAT | 2 cut(s) | 751, 1419 |
| MseI | TTAA | 6 cut(s) | 678, 759, 1073, 1091, 1119, 1160 |
| MslI | CAYNNNNRTG | 5 cut(s) | 176, 370, 1082, 1412, 1416 |
| MspI | CCGG | 1 cut(s) | 161 |
| MspR9I | CCNGG | 7 cut(s) | 15, 162, 318, 597, 795, 1034, 1572 |
| MssI | GTTTAAAC | 1 cut(s) | 1161 |
| MunI | CAATTG | 1 cut(s) | 711 |
| Mva1269I | GAATGC | 1 cut(s) | 71 |
| MvaI | CCWGG | 6 cut(s) | 15, 318, 597, 795, 1034, 1572 |
| MvnI | CGCG | 1 cut(s) | 1277 |
| NciI | CCSGG | 1 cut(s) | 162 |
| NcoI | CCATGG | 2 cut(s) | 272, 409 |
| NdeI | CATATG | 1 cut(s) | 754 |
| NdeII | GATC | 7 cut(s) | 816, 1018, 1302, 1399, 1442, 1498, 1600 |
| NlaIV | GGNNCC | 3 cut(s) | 165, 415, 1321 |
| NmuCI | GTSAC | 4 cut(s) | 232, 554, 769, 910 |
| NsiI | ATGCAT | 2 cut(s) | 751, 1419 |
| NspI | RCATGY | 4 cut(s) | 139, 380, 1417, 1693 |
| OliI | CACNNNNGTG | 1 cut(s) | 370 |
| PaeI | GCATGC | 2 cut(s) | 139, 380 |
| PaeR7I | CTCGAG | 1 cut(s) | 923 |
| PagI | TCATGA | 1 cut(s) | 783 |
| PaqCI | CACCTGC | 1 cut(s) | 1745 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1143 |
| PctI | GAATGC | 1 cut(s) | 71 |
| PfeI | GAWTC | 1 cut(s) | 1554 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1703 |
| PfoI | TCCNGGA | 1 cut(s) | 793 |
| PkrI | GCNGC | 4 cut(s) | 51, 856, 1003, 1153 |
| PleI | GAGTC | 1 cut(s) | 239 |
| PmeI | GTTTAAAC | 1 cut(s) | 1161 |
| PpsI | GAGTC | 1 cut(s) | 239 |
| Ppu21I | YACGTR | 3 cut(s) | 630, 1202, 1673 |
| PpuMI | RGGWCCY | 1 cut(s) | 538 |
| PshBI | ATTAAT | 1 cut(s) | 678 |
| Psp5II | RGGWCCY | 1 cut(s) | 538 |
| Psp6I | CCWGG | 6 cut(s) | 13, 316, 595, 793, 1032, 1570 |
| PspFI | CCCAGC | 2 cut(s) | 1051, 1173 |
| PspGI | CCWGG | 6 cut(s) | 13, 316, 595, 793, 1032, 1570 |
| PspN4I | GGNNCC | 3 cut(s) | 165, 415, 1321 |
| PspPI | GGNCC | 5 cut(s) | 164, 284, 393, 538, 791 |
| PspPPI | RGGWCCY | 1 cut(s) | 538 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1451 |
| RsaI | GTAC | 8 cut(s) | 93, 255, 628, 973, 1088, 1136, 1343, 1375 |
| RsaNI | GTAC | 8 cut(s) | 92, 254, 627, 972, 1087, 1135, 1342, 1374 |
| RseI | CAYNNNNRTG | 5 cut(s) | 176, 370, 1082, 1412, 1416 |
| SaqAI | TTAA | 6 cut(s) | 678, 759, 1073, 1091, 1119, 1160 |
| SatI | GCNGC | 4 cut(s) | 50, 855, 1002, 1152 |
| Sau3AI | GATC | 7 cut(s) | 816, 1018, 1302, 1399, 1442, 1498, 1600 |
| Sau96I | GGNCC | 5 cut(s) | 164, 284, 393, 538, 791 |
| SchI | GAGTC | 1 cut(s) | 240 |
| ScrFI | CCNGG | 7 cut(s) | 15, 162, 318, 597, 795, 1034, 1572 |
| SduI | GDGCHC | 1 cut(s) | 842 |
| SexAI | ACCWGGT | 1 cut(s) | 316 |
| SfaNI | GCATC | 4 cut(s) | 36, 736, 758, 1378 |
| SfcI | CTRYAG | 2 cut(s) | 1210, 1423 |
| Sfr274I | CTCGAG | 1 cut(s) | 923 |
| SinI | GGWCC | 4 cut(s) | 284, 393, 538, 791 |
| SlaI | CTCGAG | 1 cut(s) | 923 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 176, 370, 1082, 1412, 1416 |
| SmlI | CTYRAG | 3 cut(s) | 95, 923, 1025 |
| SmoI | CTYRAG | 3 cut(s) | 95, 923, 1025 |
| SnaBI | TACGTA | 2 cut(s) | 630, 1673 |
| SphI | GCATGC | 2 cut(s) | 139, 380 |
| SsiI | CCGC | 1 cut(s) | 855 |
| SspMI | CTAG | 3 cut(s) | 624, 858, 1155 |
| StyD4I | CCNGG | 7 cut(s) | 13, 160, 316, 595, 793, 1032, 1570 |
| StyI | CCWWGG | 4 cut(s) | 272, 409, 1323, 1369 |
| TaaI | ACNGT | 5 cut(s) | 196, 733, 874, 910, 1519 |
| TaiI | ACGT | 3 cut(s) | 632, 1204, 1675 |
| TaqI | TCGA | 2 cut(s) | 924, 1603 |
| TatI | WGTACW | 4 cut(s) | 91, 253, 1086, 1341 |
| TauI | GCSGC | 1 cut(s) | 857 |
| TfiI | GAWTC | 1 cut(s) | 1554 |
| Tru1I | TTAA | 6 cut(s) | 678, 759, 1073, 1091, 1119, 1160 |
| Tru9I | TTAA | 6 cut(s) | 678, 759, 1073, 1091, 1119, 1160 |
| TscAI | CASTG | 4 cut(s) | 248, 559, 736, 877 |
| TseFI | GTSAC | 4 cut(s) | 232, 554, 769, 910 |
| TseI | GCWGC | 3 cut(s) | 49, 1001, 1151 |
| Tsp45I | GTSAC | 4 cut(s) | 232, 554, 769, 910 |
| TspDTI | ATGAA | 5 cut(s) | 704, 1089, 1362, 1482, 1526 |
| TspGWI | ACGGA | 1 cut(s) | 264 |
| TspRI | CASTG | 4 cut(s) | 248, 559, 736, 877 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1703 |
| VpaK11BI | GGWCC | 4 cut(s) | 284, 393, 538, 791 |
| VspI | ATTAAT | 1 cut(s) | 678 |
| XapI | RAATTY | 6 cut(s) | 325, 699, 918, 1010, 1069, 1456 |
| XceI | RCATGY | 4 cut(s) | 139, 380, 1417, 1693 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 270, 1536 |
| XhoI | CTCGAG | 1 cut(s) | 923 |
| XspI | CTAG | 3 cut(s) | 624, 858, 1155 |
| Zsp2I | ATGCAT | 2 cut(s) | 751, 1419 |