Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0000233

Physical Location & Seq

Genomic Coordinates Forward (+)

28007490 .. 28011281

Gene Structure 3792 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1226600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1317 bp

ATGGTGGTCAACGTTATCATGAGGGAGTCAACGATGGTAAAGCCGGCGGAGGAGACGCCGAAGAAGACGTTGTGGAATTCGAACGTGGACCTGGTGATTCCGAGCATCCACACGGCTAGCGTCTATTTCTACAGGCCGAATCAAAACGGTGGGTCCAACTTCTTCGACCCGGTGGTGCTCAAGCACGCTCTCTCCAAGGCCCTTGTGCTGTTCTACCCCATGGCGAGGCGGCTGAAGCGCAATGACGACGGTCGTATCCAGATAGATTACGGCGGCCAGGGTGCTCTGTTTGTTGAGGCGGAGACGGGCTCCATTCTCGATGATTTCGGGGATTTCGCTCCAACCCTGGAGCTCCGGAGGCTCATCCTGGCTGTTGATTACTTTGCTGGCATATCGACTTATGCGCTCTTGGTGTTACAGGTCACGTACTTCAAATGCGGAGGAGTGTCACTTGGTGTTGGAATGCAGCACCATGTAGCTGATGGATTTTCTGGTCTGCACTTTATTAACACATGGTCTGATATAGCTCGTGGTCTTGACCTTACAATTCCTCCATTCATCGACAGGACATTGCTTCGTGGCCGAGACCCACCTCAGCCTGCATTCCACCACATTGAATACCAACCTGCTCCACTGATGAAAACCCCTCTGCAAAGCGCAAAACCTGGTGCTGCAGATACTACCACAGTCTCCATTTTTAGATTAACGCGGGATCAGCTCAACATCTTGAAATCCAAGTCTAAGAAAGCCGGGAATACAGTCAGCTATAGCTCATATGAGATGTTCTCAGGTCATGTTTGGAGATGTGTATGCAAGGCAAGTGAATTTCCCGATGATCAAGACACCAAACTATATATTGCCACTGATGGAAGGTCCAGACTGCAACCACCACTCCCACCTGGTTACTTTGGCAATGTGATTTTCACAACAACACCAATTGCTGTTGCGGGGGATCTCCAATCAAAACCAACTTGGTATGCTGCCAGCCGTATCCATGATGCTTTGGTCCGTATGGATGATAATTATCTTAGATCGGCCCTCGACTATCTTGAGCTTCAGCCTGATCTGTCTGCCCTTGTTCGTGGAGCTCATACATTTAGGTGTCCAAACCTGGGGATCACTAGTTGGGTTAGGCTGCCAATCCATGATGCCGACTTCGGATGGGGTCAGCCCATTTTCATGGGACCTGGTGGAATTGCATTCGAGGGGTTGGCATTTGTATTACCAAGTGCAACCAATGGTGGGAGTTTATCGCTGGCCATTTGTCTGCAATCCGAACATATGAAATCCTTTTCAAGGTTGTTGTATGACATATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006725 GO:0008150 GO:0008152 GO:0008374 GO:0009058 GO:0009698 GO:0009699 GO:0009808 GO:0009809 GO:0009889 GO:0009891 GO:0009893 GO:0009962 GO:0009963 GO:0009987 GO:0010252 GO:0016020 GO:0016740 GO:0016746 GO:0016747 GO:0019222 GO:0019438 GO:0019748 GO:0042592 GO:0044237 GO:0044249 GO:0044424 GO:0044444 GO:0044464 GO:0044550 GO:0047172 GO:0047205 GO:0048518 GO:0048878 GO:0050734 GO:0050737 GO:0050789 GO:0065007 GO:0065008 GO:0071704 GO:0080090 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00940 ko00941 ko00945 ko01100 ko01110 map00940 map00941 map00945 map01100 map01110

Pfam Domains

Protein Families

Transferase

Protein Analysis

439

Amino Acids

48.5

Weight (kDa)

7.19

Isoelectric Point (pI)

38.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Transferase	PF02458	5 - 432	6.3e-102	Transferase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000233)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G48930
fragaria_vesca	FvH4_2g37730 FvH4_3g06650 FvH4_3g06650 FvH4_3g06650 FvH4_3g06690 FvH4_3g06690 FvH4_3g06690 FvH4_3g37080 FvH4_3g44400 FvH4_3g44420 FvH4_3g44430 FvH4_3g44440 FvH4_6g28410
malus_domestica	MD09G1226600.v1.1 MD09G1230000.v1.1 MD09G1230100.v1.1 MD17G1224900.v1.1 MD17G1225100.v1.1
prunus_persica	Prupe.3G100800_v2.0.a1 Prupe.3G101000_v2.0.a1 Prupe.3G101400_v2.0.a1 Prupe.3G101500_v2.0.a1 Prupe.3G101600_v2.0.a1 Prupe.3G101900_v2.0.a1
pyrus_communis	pycom09g14560 pycom09g14850 pycom09g14880 pycom17g22900
rosa_chinensis	RchiOBHm_Chr2g0132521 RchiOBHm_Chr2g0132571 RchiOBHm_Chr2g0132581 RchiOBHm_Chr2g0132601 RchiOBHm_Chr2g0132651 RchiOBHm_Chr5g0066491 RchiOBHm_Chr5g0066541 RchiOBHm_Chr5g0066631 RchiOBHm_Chr5g0066641 RchiOBHm_Chr5g0066711 RchiOBHm_Chr5g0066721 RchiOBHm_Chr5g0066761 RchiOBHm_Chr5g0066771 RchiOBHm_Chr5g0066791 RchiOBHm_Chr5g0066811 RchiOBHm_Chr5g0081061 RchiOBHm_Chr5g0081111
rosa_laevigata	RLG00000019264 RLG00000019269 RLG00000019270 RLG00000035871 RLG00000035873 RLG00000035874 RLG00000035875 RLG00000036910
rosa_multiflora	Rmu_co8101688.1_g000001 Rmu_co8204910.1_g000001 Rmu_co8308169.1_g000001 Rmu_sc0000605.1_g000027 Rmu_sc0000605.1_g000029 Rmu_sc0000605.1_g000045 Rmu_sc0000605.1_g000049 Rmu_sc0005460.1_g000001 Rmu_sc0005460.1_g000009 Rmu_sc0008823.1_g000006 Rmu_sc0012629.1_g000001 Rmu_sc0014046.1_g000003 Rmu_sc0025032.1_g000001 Rmu_ssc0000074.1_g000018 Rmu_ssc0000074.1_g000029 Rmu_ssc0000432.1_g000085 Rmu_ssc0000432.1_g000114
rosa_roxburghii	Rroxscaffold_1G00002330 Rroxscaffold_1G00002370 Rroxscaffold_1G00014290 Rroxscaffold_1G00014320 Rroxscaffold_1G00014350 Rroxscaffold_1G00014370 Rroxscaffold_1G00014380 Rroxscaffold_1G00014500 Rroxscaffold_1G00014530 Rroxscaffold_1G00014550 Rroxscaffold_2G00111750 Rroxscaffold_2G00111800 Rroxscaffold_2G00111850
rosa_rugosa	Rorug02G0305200 Rorug02G0305300 Rorug02G0305900 Rorug02G0305900 Rorug05G0378800 Rorug05G0379000 Rorug05G0379000 Rorug05G0473400
rosa_samantha	Rh2AG358000 Rh2AG358100 Rh2AG358200 Rh2AG358300 Rh2AG358600 Rh2AG358700 Rh2AG358900 Rh2BG364400 Rh2BG364900 Rh2BG365000 Rh2BG365100 Rh2BG365500 Rh2CG341500 Rh2CG342100 Rh2DG381100 Rh2DG381500 Rh2DG381800 Rh5AG525900 Rh5BG453900 Rh5BG454100 Rh5BG454200 Rh5BG454500 Rh5BG454700 Rh5BG455100 Rh5BG455300 Rh5BG549800 Rh5BG550200 Rh5CG476200 Rh5CG476400 Rh5CG476500 Rh5CG476800 Rh5CG477000 Rh5CG477200 Rh5CG477400 Rh5CG572500 Rh5CG573100 Rh5DG469000 Rh5DG469200 Rh5DG469900 Rh5DG470000 Rh5DG470100 Rh5DG559900
rosa_wichuraiana	Rw0G002550 Rw0G012040 Rw2G029120 Rw2G029210 Rw5G040950 Rw5G040970 Rw5G048860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	634
AccII	CGCG	1 cut(s)	709
AccIII	TCCGGA	1 cut(s)	354
AciI	CCGC	7 cut(s)	47, 229, 273, 299, 438, 709, 947
AclI	AACGTT	1 cut(s)	12
AclWI	GGATC	3 cut(s)	720, 960, 1124
AcoI	YGGCCR	3 cut(s)	274, 580, 1257
AcsI	RAATTY	2 cut(s)	76, 824
AcuI	CTGAAG	2 cut(s)	254, 1040
AcyI	GRCGYC	1 cut(s)	56
AdeI	CACNNNGTG	1 cut(s)	455
AfaI	GTAC	1 cut(s)	428
AfiI	CCNNNNNNNGG	4 cut(s)	225, 1112, 1242, 1296
AgsI	TTSAA	4 cut(s)	433, 617, 730, 1296
AhlI	ACTAGT	1 cut(s)	1121
AjnI	CCWGG	8 cut(s)	90, 276, 345, 366, 664, 898, 1110, 1186
AluBI	AGCT	8 cut(s)	352, 479, 527, 718, 765, 771, 1054, 1088
AluI	AGCT	8 cut(s)	352, 479, 527, 718, 765, 771, 1054, 1088
Alw21I	GWGCWC	4 cut(s)	180, 286, 354, 1090
Alw26I	GTCTC	4 cut(s)	47, 296, 579, 694
AlwI	GGATC	3 cut(s)	720, 960, 1124
Aor13HI	TCCGGA	1 cut(s)	354
AoxI	GGCC	6 cut(s)	134, 198, 274, 580, 1035, 1257
ApeKI	GCWGC	4 cut(s)	466, 671, 980, 1135
ApoI	RAATTY	2 cut(s)	76, 824
AspLEI	GCGC	3 cut(s)	240, 406, 659
AspS9I	GGNCC	7 cut(s)	88, 153, 199, 873, 1006, 1036, 1184
AsuC2I	CCSGG	2 cut(s)	170, 751
AsuHPI	GGTGA	1 cut(s)	106
AsuII	TTCGAA	1 cut(s)	80
AsuNHI	GCTAGC	1 cut(s)	116
AvaII	GGWCC	5 cut(s)	88, 153, 873, 1006, 1184
BaeI	ACNNNNGTAYC	2 cut(s)	238, 271
BalI	TGGCCA	1 cut(s)	1259
BanII	GRGCYC	3 cut(s)	311, 354, 1090
BauI	CACGAG	1 cut(s)	528
BbsI	GAAGAC	1 cut(s)	71
Bbv12I	GWGCWC	4 cut(s)	180, 286, 354, 1090
BbvCI	CCTCAGC	1 cut(s)	594
BbvI	GCAGC	4 cut(s)	478, 658, 967, 1122
BccI	CCATC	4 cut(s)	28, 476, 860, 1155
BceAI	ACGGC	3 cut(s)	129, 286, 972
BciT130I	CCWGG	8 cut(s)	92, 278, 347, 368, 666, 900, 1112, 1188
BciVI	GTATCC	2 cut(s)	266, 1001
BclI	TGATCA	1 cut(s)	835
BcnI	CCSGG	2 cut(s)	170, 751
BcoDI	GTCTC	4 cut(s)	47, 296, 579, 694
BcuI	ACTAGT	1 cut(s)	1121
BfaI	CTAG	2 cut(s)	117, 1122
BfmI	CTRYAG	3 cut(s)	130, 672, 766
BfuAI	ACCTGC	1 cut(s)	634
BfuI	GTATCC	2 cut(s)	266, 1001
BisI	GCNGC	6 cut(s)	230, 274, 467, 672, 981, 1136
BlsI	GCNGC	6 cut(s)	231, 275, 468, 673, 982, 1137
Bme18I	GGWCC	5 cut(s)	88, 153, 873, 1006, 1184
BmgT120I	GGNCC	7 cut(s)	88, 153, 199, 873, 1006, 1036, 1184
BmiI	GGNNCC	3 cut(s)	154, 310, 1185
BmsI	GCATC	3 cut(s)	114, 988, 1138
BmtI	GCTAGC	1 cut(s)	120
BoxI	GACNNNNGTC	1 cut(s)	249
BpiI	GAAGAC	1 cut(s)	71
BplI	GAGNNNNNCTC	4 cut(s)	293, 325, 770, 802
BpmI	CTGGAG	1 cut(s)	368
Bpu10I	CCTNAGC	1 cut(s)	594
Bpu14I	TTCGAA	1 cut(s)	80
BpuEI	CTTGAG	2 cut(s)	164, 1070
BpuMI	CCSGG	2 cut(s)	170, 751
BsaAI	YACGTR	1 cut(s)	426
BsaBI	GATNNNNATC	1 cut(s)	1023
BsaHI	GRCGYC	1 cut(s)	56
BsaI	GGTCTC	1 cut(s)	579
BsaJI	CCNNGG	5 cut(s)	195, 219, 277, 345, 1111
BsaWI	WCCGGW	1 cut(s)	354
BsaXI	ACNNNNNCTCC	6 cut(s)	176, 206, 535, 565, 876, 906
Bsc4I	CCNNNNNNNGG	4 cut(s)	225, 1112, 1242, 1296
Bse118I	RCCGGY	1 cut(s)	43
Bse3DI	GCAATG	3 cut(s)	247, 569, 919
Bse8I	GATNNNNATC	1 cut(s)	1023
BseAI	TCCGGA	1 cut(s)	354
BseBI	CCWGG	8 cut(s)	92, 278, 347, 368, 666, 900, 1112, 1188
BseDI	CCNNGG	5 cut(s)	195, 219, 277, 345, 1111
BseGI	GGATG	4 cut(s)	105, 363, 1021, 1166
BseJI	GATNNNNATC	1 cut(s)	1023
BseLI	CCNNNNNNNGG	4 cut(s)	225, 1112, 1242, 1296
BseMI	GCAATG	3 cut(s)	247, 569, 919
BseMII	CTCAG	2 cut(s)	608, 801
BseRI	GAGGAG	2 cut(s)	65, 456
BseXI	GCAGC	4 cut(s)	478, 658, 967, 1122
BsgI	GTGCAG	1 cut(s)	482
Bsh1236I	CGCG	1 cut(s)	709
Bsh1285I	CGRYCG	1 cut(s)	253
BshFI	GGCC	6 cut(s)	136, 200, 276, 582, 1037, 1259
BsiEI	CGRYCG	1 cut(s)	253
BsiHKAI	GWGCWC	4 cut(s)	180, 286, 354, 1090
BsiSI	CCGG	4 cut(s)	44, 170, 355, 750
BslFI	GGGAC	1 cut(s)	1197
BslI	CCNNNNNNNGG	4 cut(s)	225, 1112, 1242, 1296
BsmAI	GTCTC	4 cut(s)	47, 296, 579, 694
BsmBI	CGTCTC	2 cut(s)	47, 296
BsmFI	GGGAC	1 cut(s)	1197
BsmI	GAATGC	3 cut(s)	468, 602, 1199
BsnI	GGCC	6 cut(s)	136, 200, 276, 582, 1037, 1259
Bso31I	GGTCTC	1 cut(s)	579
Bsp119I	TTCGAA	1 cut(s)	80
Bsp1286I	GDGCHC	5 cut(s)	180, 286, 311, 354, 1090
Bsp13I	TCCGGA	1 cut(s)	354
Bsp143I	GATC	6 cut(s)	712, 835, 952, 1031, 1063, 1116
Bsp19I	CCATGG	1 cut(s)	219
BspACI	CCGC	7 cut(s)	47, 229, 273, 299, 438, 709, 947
BspANI	GGCC	6 cut(s)	136, 200, 276, 582, 1037, 1259
BspCNI	CTCAG	2 cut(s)	607, 800
BspEI	TCCGGA	1 cut(s)	354
BspFNI	CGCG	1 cut(s)	709
BspHI	TCATGA	1 cut(s)	18
BspLI	GGNNCC	3 cut(s)	154, 310, 1185
BspMAI	CTGCAG	1 cut(s)	676
BspMI	ACCTGC	1 cut(s)	634
BspOI	GCTAGC	1 cut(s)	120
BspPI	GGATC	3 cut(s)	720, 960, 1124
BspT104I	TTCGAA	1 cut(s)	80
BspTNI	GGTCTC	1 cut(s)	579
BsrDI	GCAATG	3 cut(s)	247, 569, 919
BsrFI	RCCGGY	1 cut(s)	43
BssAI	RCCGGY	1 cut(s)	43
BssECI	CCNNGG	5 cut(s)	195, 219, 277, 345, 1111
BssMI	GATC	6 cut(s)	712, 835, 952, 1031, 1063, 1116
BssNI	GRCGYC	1 cut(s)	56
BssSI	CACGAG	1 cut(s)	528
BssT1I	CCWWGG	2 cut(s)	195, 219
Bst2BI	CACGAG	1 cut(s)	528
Bst2UI	CCWGG	8 cut(s)	92, 278, 347, 368, 666, 900, 1112, 1188
Bst4CI	ACNGT	4 cut(s)	149, 251, 688, 760
BstACI	GRCGYC	1 cut(s)	56
BstBAI	YACGTR	1 cut(s)	426
BstBI	TTCGAA	1 cut(s)	80
BstC8I	GCNNGC	7 cut(s)	45, 118, 186, 388, 600, 985, 1257
BstDEI	CTNAG	4 cut(s)	594, 741, 787, 1028
BstDSI	CCRYGG	1 cut(s)	219
BstENI	CCTNNNNNAGG	1 cut(s)	1294
BstF5I	GGATG	4 cut(s)	105, 363, 1021, 1166
BstFNI	CGCG	1 cut(s)	709
BstHHI	GCGC	3 cut(s)	240, 406, 659
BstKTI	GATC	6 cut(s)	715, 838, 955, 1034, 1066, 1119
BstMAI	GTCTC	4 cut(s)	47, 296, 579, 694
BstMBI	GATC	6 cut(s)	712, 835, 952, 1031, 1063, 1116
BstMCI	CGRYCG	1 cut(s)	253
BstMWI	GCNNNNNNNGC	3 cut(s)	235, 358, 715
BstNI	CCWGG	8 cut(s)	92, 278, 347, 368, 666, 900, 1112, 1188
BstPAI	GACNNNNGTC	1 cut(s)	249
BstSFI	CTRYAG	3 cut(s)	130, 672, 766
BstUI	CGCG	1 cut(s)	709
BstV1I	GCAGC	4 cut(s)	478, 658, 967, 1122
BstV2I	GAAGAC	1 cut(s)	71
BstX2I	RGATCY	1 cut(s)	952
BstXI	CCANNNNNNTGG	1 cut(s)	1180
BstYI	RGATCY	1 cut(s)	952
BsuI	GTATCC	2 cut(s)	266, 1001
BsuRI	GGCC	6 cut(s)	136, 200, 276, 582, 1037, 1259
BtgI	CCRYGG	1 cut(s)	219
BtsCI	GGATG	4 cut(s)	105, 363, 1021, 1166
BtsIMutI	CAGTG	2 cut(s)	632, 861
BveI	ACCTGC	1 cut(s)	634
Cac8I	GCNNGC	7 cut(s)	45, 118, 186, 388, 600, 985, 1257
CciI	TCATGA	1 cut(s)	18
CfoI	GCGC	3 cut(s)	240, 406, 659
Cfr10I	RCCGGY	1 cut(s)	43
Cfr13I	GGNCC	7 cut(s)	88, 153, 199, 873, 1006, 1036, 1184
CseI	GACGC	2 cut(s)	64, 109
CsiI	ACCWGGT	4 cut(s)	90, 664, 898, 1186
Csp6I	GTAC	1 cut(s)	427
CviAII	CATG	8 cut(s)	19, 220, 473, 513, 794, 995, 1145, 1180
CviQI	GTAC	1 cut(s)	427
DdeI	CTNAG	4 cut(s)	594, 741, 787, 1028
DpnI	GATC	6 cut(s)	714, 837, 954, 1033, 1065, 1118
DpnII	GATC	6 cut(s)	712, 835, 952, 1031, 1063, 1116
DraIII	CACNNNGTG	1 cut(s)	455
EaeI	YGGCCR	3 cut(s)	274, 580, 1257
EciI	GGCGGA	2 cut(s)	62, 314
Ecl136II	GAGCTC	2 cut(s)	352, 1088
Eco130I	CCWWGG	2 cut(s)	195, 219
Eco24I	GRGCYC	3 cut(s)	311, 354, 1090
Eco31I	GGTCTC	1 cut(s)	579
Eco47I	GGWCC	5 cut(s)	88, 153, 873, 1006, 1184
Eco53kI	GAGCTC	2 cut(s)	352, 1088
Eco57I	CTGAAG	2 cut(s)	254, 1040
EcoICRI	GAGCTC	2 cut(s)	352, 1088
EcoNI	CCTNNNNNAGG	1 cut(s)	1294
EcoO109I	RGGNCCY	2 cut(s)	199, 1184
EcoRI	GAATTC	1 cut(s)	76
EcoRII	CCWGG	8 cut(s)	90, 276, 345, 366, 664, 898, 1110, 1186
EcoT14I	CCWWGG	2 cut(s)	195, 219
EcoT38I	GRGCYC	3 cut(s)	311, 354, 1090
ErhI	CCWWGG	2 cut(s)	195, 219
Esp3I	CGTCTC	2 cut(s)	47, 296
FaeI	CATG	8 cut(s)	22, 223, 476, 516, 797, 998, 1148, 1183
FaqI	GGGAC	1 cut(s)	1197
FatI	CATG	8 cut(s)	18, 219, 472, 512, 793, 994, 1144, 1179
FauI	CCCGC	2 cut(s)	702, 940
FauNDI	CATATG	2 cut(s)	775, 1281
FbaI	TGATCA	1 cut(s)	835
Fnu4HI	GCNGC	6 cut(s)	230, 274, 467, 672, 981, 1136
FokI	GGATG	4 cut(s)	92, 350, 1028, 1173
FriOI	GRGCYC	3 cut(s)	311, 354, 1090
Fsp4HI	GCNGC	6 cut(s)	230, 274, 467, 672, 981, 1136
FspBI	CTAG	2 cut(s)	117, 1122
GlaI	GCGC	3 cut(s)	239, 405, 658
GluI	GCNGC	6 cut(s)	230, 274, 467, 672, 981, 1136
GsuI	CTGGAG	1 cut(s)	368
HaeIII	GGCC	6 cut(s)	136, 200, 276, 582, 1037, 1259
HapII	CCGG	4 cut(s)	44, 170, 355, 750
HgaI	GACGC	2 cut(s)	64, 109
HhaI	GCGC	3 cut(s)	240, 406, 659
Hin1I	GRCGYC	1 cut(s)	56
Hin1II	CATG	8 cut(s)	22, 223, 476, 516, 797, 998, 1148, 1183
Hin6I	GCGC	3 cut(s)	238, 404, 657
HinP1I	GCGC	3 cut(s)	238, 404, 657
HincII	GTYRAC	2 cut(s)	10, 30
HindII	GTYRAC	2 cut(s)	10, 30
HinfI	GANTC	3 cut(s)	26, 97, 139
HpaII	CCGG	4 cut(s)	44, 170, 355, 750
HphI	GGTGA	1 cut(s)	106
Hpy166II	GTNNAC	3 cut(s)	10, 30, 88
Hpy188I	TCNGA	4 cut(s)	102, 520, 1160, 1276
Hpy8I	GTNNAC	3 cut(s)	10, 30, 88
Hpy99I	CGWCG	1 cut(s)	251
HpyAV	CCTTC	1 cut(s)	864
HpyCH4III	ACNGT	4 cut(s)	149, 251, 688, 760
HpyCH4IV	ACGT	4 cut(s)	12, 68, 84, 425
HpyF10VI	GCNNNNNNNGC	3 cut(s)	235, 358, 715
HpyF3I	CTNAG	4 cut(s)	594, 741, 787, 1028
HpySE526I	ACGT	4 cut(s)	12, 68, 84, 425
Hsp92I	GRCGYC	1 cut(s)	56
Hsp92II	CATG	8 cut(s)	22, 223, 476, 516, 797, 998, 1148, 1183
HspAI	GCGC	3 cut(s)	238, 404, 657
Kpn2I	TCCGGA	1 cut(s)	354
KroI	GCCGGC	1 cut(s)	43
KroNI	GCCGGC	1 cut(s)	45
Ksp22I	TGATCA	1 cut(s)	835
Kzo9I	GATC	6 cut(s)	712, 835, 952, 1031, 1063, 1116
LmnI	GCTCC	6 cut(s)	314, 343, 349, 357, 634, 1085
Lsp1109I	GCAGC	4 cut(s)	478, 658, 967, 1122
LweI	GCATC	3 cut(s)	114, 988, 1138
MabI	ACCWGGT	4 cut(s)	90, 664, 898, 1186
MaeI	CTAG	2 cut(s)	117, 1122
MaeII	ACGT	4 cut(s)	12, 68, 84, 425
MaeIII	GTNAC	4 cut(s)	414, 421, 447, 902
MalI	GATC	6 cut(s)	714, 837, 954, 1033, 1065, 1118
MboI	GATC	6 cut(s)	712, 835, 952, 1031, 1063, 1116
MboII	GAAGA	3 cut(s)	73, 76, 154
MfeI	CAATTG	1 cut(s)	936
MflI	RGATCY	1 cut(s)	952
MhlI	GDGCHC	5 cut(s)	180, 286, 311, 354, 1090
MlsI	TGGCCA	1 cut(s)	1259
MluCI	AATT	6 cut(s)	76, 546, 824, 936, 1021, 1194
MluNI	TGGCCA	1 cut(s)	1259
MlyI	GAGTC	1 cut(s)	35
MmeI	TCCRAC	3 cut(s)	180, 365, 439
Mox20I	TGGCCA	1 cut(s)	1259
MroI	TCCGGA	1 cut(s)	354
MroNI	GCCGGC	1 cut(s)	43
MscI	TGGCCA	1 cut(s)	1259
MseI	TTAA	2 cut(s)	507, 704
MslI	CAYNNNNRTG	2 cut(s)	1099, 1178
Msp20I	TGGCCA	1 cut(s)	1259
MspI	CCGG	4 cut(s)	44, 170, 355, 750
MunI	CAATTG	1 cut(s)	936
Mva1269I	GAATGC	3 cut(s)	468, 602, 1199
MvaI	CCWGG	8 cut(s)	92, 278, 347, 368, 666, 900, 1112, 1188
MvnI	CGCG	1 cut(s)	709
MwoI	GCNNNNNNNGC	3 cut(s)	235, 358, 715
NaeI	GCCGGC	1 cut(s)	45
NciI	CCSGG	2 cut(s)	170, 751
NcoI	CCATGG	1 cut(s)	219
NdeI	CATATG	2 cut(s)	775, 1281
NdeII	GATC	6 cut(s)	712, 835, 952, 1031, 1063, 1116
NgoMIV	GCCGGC	1 cut(s)	43
NheI	GCTAGC	1 cut(s)	116
NlaIII	CATG	8 cut(s)	22, 223, 476, 516, 797, 998, 1148, 1183
NlaIV	GGNNCC	3 cut(s)	154, 310, 1185
NmeAIII	GCCGAG	1 cut(s)	608
NmuCI	GTSAC	2 cut(s)	421, 447
NspV	TTCGAA	1 cut(s)	80
PagI	TCATGA	1 cut(s)	18
PctI	GAATGC	3 cut(s)	468, 602, 1199
PdiI	GCCGGC	1 cut(s)	45
PfeI	GAWTC	2 cut(s)	97, 139
PkrI	GCNGC	6 cut(s)	231, 275, 468, 673, 982, 1137
PleI	GAGTC	1 cut(s)	34
PpsI	GAGTC	1 cut(s)	34
Ppu21I	YACGTR	1 cut(s)	426
PpuMI	RGGWCCY	1 cut(s)	1184
PshAI	GACNNNNGTC	1 cut(s)	249
Psp124BI	GAGCTC	2 cut(s)	354, 1090
Psp1406I	AACGTT	1 cut(s)	12
Psp5II	RGGWCCY	1 cut(s)	1184
Psp6I	CCWGG	8 cut(s)	90, 276, 345, 366, 664, 898, 1110, 1186
PspGI	CCWGG	8 cut(s)	90, 276, 345, 366, 664, 898, 1110, 1186
PspN4I	GGNNCC	3 cut(s)	154, 310, 1185
PspPI	GGNCC	7 cut(s)	88, 153, 199, 873, 1006, 1036, 1184
PspPPI	RGGWCCY	1 cut(s)	1184
PstI	CTGCAG	1 cut(s)	676
PsuI	RGATCY	1 cut(s)	952
RsaI	GTAC	1 cut(s)	428
RsaNI	GTAC	1 cut(s)	427
RseI	CAYNNNNRTG	2 cut(s)	1099, 1178
SacI	GAGCTC	2 cut(s)	354, 1090
SaqAI	TTAA	2 cut(s)	507, 704
SatI	GCNGC	6 cut(s)	230, 274, 467, 672, 981, 1136
Sau3AI	GATC	6 cut(s)	712, 835, 952, 1031, 1063, 1116
Sau96I	GGNCC	7 cut(s)	88, 153, 199, 873, 1006, 1036, 1184
SchI	GAGTC	1 cut(s)	35
SduI	GDGCHC	5 cut(s)	180, 286, 311, 354, 1090
SexAI	ACCWGGT	4 cut(s)	90, 664, 898, 1186
SfaNI	GCATC	3 cut(s)	114, 988, 1138
SfcI	CTRYAG	3 cut(s)	130, 672, 766
SfuI	TTCGAA	1 cut(s)	80
SinI	GGWCC	5 cut(s)	88, 153, 873, 1006, 1184
SmiMI	CAYNNNNRTG	2 cut(s)	1099, 1178
SmlI	CTYRAG	2 cut(s)	179, 1049
SmoI	CTYRAG	2 cut(s)	179, 1049
SpeI	ACTAGT	1 cut(s)	1121
Sse9I	AATT	6 cut(s)	76, 546, 824, 936, 1021, 1194
SsiI	CCGC	7 cut(s)	47, 229, 273, 299, 438, 709, 947
SspMI	CTAG	2 cut(s)	117, 1122
SstI	GAGCTC	2 cut(s)	354, 1090
StyI	CCWWGG	2 cut(s)	195, 219
TaaI	ACNGT	4 cut(s)	149, 251, 688, 760
TaiI	ACGT	4 cut(s)	15, 71, 87, 428
TaqI	TCGA	7 cut(s)	80, 165, 318, 395, 561, 1041, 1203
TasI	AATT	6 cut(s)	76, 546, 824, 936, 1021, 1194
TauI	GCSGC	2 cut(s)	232, 276
TfiI	GAWTC	2 cut(s)	97, 139
Tru1I	TTAA	2 cut(s)	507, 704
Tru9I	TTAA	2 cut(s)	507, 704
TscAI	CASTG	2 cut(s)	639, 868
TseFI	GTSAC	2 cut(s)	421, 447
TseI	GCWGC	4 cut(s)	466, 671, 980, 1135
Tsp45I	GTSAC	2 cut(s)	421, 447
TspDTI	ATGAA	4 cut(s)	547, 653, 1168, 1298
TspGWI	ACGGA	1 cut(s)	998
TspRI	CASTG	2 cut(s)	639, 868
VpaK11BI	GGWCC	5 cut(s)	88, 153, 873, 1006, 1184
XagI	CCTNNNNNAGG	1 cut(s)	1294
XapI	RAATTY	2 cut(s)	76, 824
XcmI	CCANNNNNNNNNTGG	1 cut(s)	479
XspI	CTAG	2 cut(s)	117, 1122

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.