MD10G1002600.v1.1

eukaryotic translation initiation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000686
Physical Location & Seq
Reverse (-)
584680 .. 590671
5992 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1002600.v1.1.491

Sequence Viewer

Length: 1836 bp
ATGTTCAAGAAGGCAGTTGAAGCAAGGTCACACCAAAGACTCTCAGGGGCTGACAGGAAGAAGCTCAAGAGGAATCTCAAAGACAAATTTCCCCGAGCTTCCGACTCCGATATCGATTCTTTACTCCCTCCCAAGGTGGAGATATCAGTTTCAAAGTTTCAAAATCGTGTTCATGTATATGGGGTGGAGGGGGGCTTTCCTATATTTTTTGATATTGATGGGCGCGGCACAGACATTTTTCCCACAGTTTATGCACTCTGGAAGGTCCCTGAGCTCTTGCCTTCTTTCATGCTGAAAGGGGGTGAGGTTTCCCGATATATCATTGGAGGGGCGGATTTGATGTTCCCTGGTATCAGCATACCTGCTGAAGGTCTACCTTCTTTTACAGCAGGAGAAACCTGGGCAGTAAAAGTTCCAGGAAACCCAGCTCCCATCGCTGTGGGGACTACCACTATGAGCAGCACTGAAGCGTTAAAAGCCGGGTTGCGAGGAAAGGCTTTAAAGATTACTCACTATTATCGTGACTTACTTTGGGAATCAGTTGAGGGCCCTTATGTTCCAAATGCAGGCTTTTATGAAGATGTTGTGTTTGAGGATCCTACCTTGTCATCATCGCAAATTTCTGAATCTTATGCTAGCACAACTGAAGGTTCAAATGACCAAGAGAATGGAAGTAATGAAAAAGAAATTGGACAACCTGTTGATGTGGACGTGCACTCTGAACCTAGTTCCACTTCAGAGGCACAGATCGAGGTTGGGGACGAGATTGCTAAAGAAATAACTGCACATGTAGGTGATCTGAAGGTAACAGATAATCATTCTGCTGATCAGTCTAATGGGGAGGATCAACATCCTCTTTCCACTGAGGATGTGGATATGCTTCTGGAAAAATGCCTTTTGCAAGCATTGCATACAACTATTAAGGATAAAGACCTTCCTATGCCTGGCAGCACACTGTGGTCAAACCACGTTCTACCTTGTAGGCCTTCAGGCATCACACTCGACATCAAGAAATCATCATACAAGAAATTATCAAAGTGGTTACAAGCTAAATCTTCTACAGGCTTGATTTCAGTGAAAGAAGATAAATACAAAAAGGAGTCTGTATTGTTATCGGTTAACCGCAAGCATTCAGATTACTCATCTTTTAAGCCTGAGAAACGAGAAGTGGAGAAAACTGTTCAAACTGGTGTTGCTACTGTCAGTGAAGGGCGGTCACTCAAAATGCTTGAAGTGGCTGAGATCTATAAATCAAGTGTACATGTCAATCCCATACTAGCTTCTGTTGGGGCCGACACAGGAAAGCTTTACAGTGCTTCAGATGCCACAGATATTGTGTTTACATATGTTGAAAAAGAAAACCTGGTTAAGCCCTTAGATAAATCCATTGTGGTGTTGGATCCAATATTATGTGATGCACTATTCAAGGGAGCCATAAAAAAAGGTGCAATGTACCCAACAGAAATTCATAAGAGAGATTTAGGAGCAGCATTTGTAAACAGGATGCAAGCCCATCACGTTGTGACGAGGGGAAGTGAGTCGGTTGTTCGTAAAGGTGGTCTGAAAACAATACAGATAATGACAGAAAGGCGGCAAGGTAACAAGAAAGTGACCAAATTATCAGGTCTGGAATCCTTTTTGGTGGATCCTGAAGCTTTGGCATCAGAGCTACAGAAGAAGTTTGCTTGCAGCACAACGGTGTCTGAACTACCAGGTAAGAAAGGGCATGAGGTTCTGGTTCAAGGTGGCGTGATTGATGATCTGGCAAAGCATATGATTGAACAATATGGAATCCCAAAGAGATACATAGAGGTTCTTGATAAAACCAGGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SUI1

Protein Analysis

612

Amino Acids

67.15

Weight (kDa)

6.63

Isoelectric Point (pI)

37.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pre-PUA PF17832 2 - 90 5.7e-19 Pre-PUA-like domain
PUA_elF2D PF26292 101 - 175 6e-23 elF2D, PUA domain
WHD_eIF2D PF25304 292 - 374 1.4e-29 eIF2D winged helix domain
SWIB_eIF2D PF26291 416 - 476 3.3e-11 eIF2D, SWIB domain
SUI1 PF01253 522 - 599 3.6e-22 Translation initiation factor SUI1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000686)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71350
fragaria_vesca FvH4_2g00430 FvH4_2g00430 FvH4_2g00430 FvH4_2g00430
malus_domestica MD10G1002600.v1.1
prunus_persica Prupe.8G004200_v2.0.a1 Prupe.8G004200_v2.0.a1 Prupe.8G004200_v2.0.a1 Prupe.8G004200_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0249251 RchiOBHm_Chr6g0249261 RchiOBHm_Chr6g0249701 RchiOBHm_Chr6g0249761 RchiOBHm_Chr6g0249851 RchiOBHm_Chr6g0249861 RchiOBHm_Chr6g0249871 RchiOBHm_Chr6g0249891 RchiOBHm_Chr6g0249901 RchiOBHm_Chr6g0249911 RchiOBHm_Chr6g0249981 RchiOBHm_Chr6g0249991 RchiOBHm_Chr6g0250001 RchiOBHm_Chr6g0250151 RchiOBHm_Chr6g0250171 RchiOBHm_Chr6g0250191 RchiOBHm_Chr6g0250211 RchiOBHm_Chr6g0250221 RchiOBHm_Chr6g0250321 RchiOBHm_Chr6g0250391
rosa_laevigata RLG00000015543 RLG00000015548
rosa_multiflora Rmu_sc0000411.1_g000004 Rmu_sc0000411.1_g000009 Rmu_sc0000704.1_g000007 Rmu_sc0000704.1_g000011 Rmu_sc0001209.1_g000038 Rmu_sc0001209.1_g000042 Rmu_sc0001209.1_g000048 Rmu_sc0003293.1_g000005 Rmu_sc0004691.1_g000005 Rmu_sc0004691.1_g000007 Rmu_sc0004691.1_g000009 Rmu_sc0006032.1_g000001 Rmu_sc0006032.1_g000004 Rmu_sc0006032.1_g000005 Rmu_sc0008209.1_g000003 Rmu_sc0008209.1_g000010 Rmu_sc0009002.1_g000001 Rmu_sc0009002.1_g000005 Rmu_sc0009002.1_g000009
rosa_roxburghii Rroxscaffold_3G00218110 Rroxscaffold_7G00217270 Rroxscaffold_7G00217290 Rroxscaffold_7G00217300 Rroxscaffold_7G00217340
rosa_rugosa Rorug05G0494700 Rorug05G0495000 Rorug05G0496000 Rorug05G0498100 Rorug05G0498200
rosa_samantha Rh6AG000200 Rh6AG000300 Rh6AG004700 Rh6AG004800 Rh6AG005200 Rh6AG005300 Rh6AG005600 Rh6BG005500 Rh6BG005600 Rh6CG003700 Rh6DG003600
rosa_wichuraiana Rw6G000560 Rw6G000600 Rw6G000620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 370
AccI GTMKAC 1 cut(s) 373
AccII CGCG 1 cut(s) 225
AciI CCGC 5 cut(s) 225, 332, 1123, 1213, 1591
AclWI GGATC 7 cut(s) 590, 603, 852, 1394, 1407, 1640, 1653
AcsI RAATTY 3 cut(s) 86, 618, 1464
AcuI CTGAAG 8 cut(s) 387, 486, 666, 720, 821, 972, 1302, 1671
AdeI CACNNNGTG 2 cut(s) 957, 1522
AfaI GTAC 2 cut(s) 1260, 1454
AfiI CCNNNNNNNGG 4 cut(s) 133, 368, 566, 944
AflIII ACRYGT 2 cut(s) 787, 1261
AjiI CACGTC 1 cut(s) 712
AjnI CCWGG 7 cut(s) 346, 398, 415, 943, 1362, 1711, 1826
AjuI GAANNNNNNNTTGG 2 cut(s) 672, 704
AleI CACNNNNGTG 1 cut(s) 1697
AloI GAACNNNNNNTCC 2 cut(s) 326, 358
AluBI AGCT 9 cut(s) 64, 98, 274, 428, 1049, 1280, 1306, 1655, 1669
AluI AGCT 9 cut(s) 64, 98, 274, 428, 1049, 1280, 1306, 1655, 1669
Alw21I GWGCWC 2 cut(s) 276, 717
Alw44I GTGCAC 1 cut(s) 713
AlwI GGATC 7 cut(s) 590, 603, 852, 1394, 1407, 1640, 1653
AlwNI CAGNNNCTG 1 cut(s) 50
Ama87I CYCGRG 1 cut(s) 93
AoxI GGCC 3 cut(s) 547, 983, 1290
ApaI GGGCCC 1 cut(s) 551
ApaLI GTGCAC 1 cut(s) 713
ApeKI GCWGC 4 cut(s) 459, 948, 1487, 1689
ApoI RAATTY 3 cut(s) 86, 618, 1464
AspLEI GCGC 1 cut(s) 225
AspS9I GGNCC 4 cut(s) 265, 547, 548, 1290
AsuC2I CCSGG 1 cut(s) 481
AsuHPI GGTGA 2 cut(s) 314, 806
AsuNHI GCTAGC 1 cut(s) 635
AvaI CYCGRG 1 cut(s) 93
AvaII GGWCC 1 cut(s) 265
BaeGI GKGCMC 2 cut(s) 551, 717
BamHI GGATCC 3 cut(s) 595, 1399, 1645
BanII GRGCYC 2 cut(s) 276, 551
Bbv12I GWGCWC 2 cut(s) 276, 717
BbvI GCAGC 4 cut(s) 471, 960, 1499, 1701
BccI CCATC 3 cut(s) 212, 440, 1521
BcgI CGANNNNNNTGC 2 cut(s) 982, 1016
BciT130I CCWGG 7 cut(s) 348, 400, 417, 945, 1364, 1713, 1828
BclI TGATCA 1 cut(s) 826
BcnI CCSGG 1 cut(s) 481
BfaI CTAG 3 cut(s) 636, 726, 1277
BfmI CTRYAG 2 cut(s) 1059, 1670
BfuAI ACCTGC 1 cut(s) 370
BglII AGATCT 1 cut(s) 1242
BisI GCNGC 6 cut(s) 226, 460, 949, 1488, 1592, 1690
BlsI GCNGC 6 cut(s) 227, 461, 950, 1489, 1593, 1691
Bme1390I CCNGG 8 cut(s) 348, 400, 417, 481, 945, 1364, 1713, 1828
Bme18I GGWCC 1 cut(s) 265
BmeT110I CYCGRG 1 cut(s) 93
BmgBI CACGTC 1 cut(s) 712
BmgT120I GGNCC 4 cut(s) 265, 547, 548, 1290
BmiI GGNNCC 7 cut(s) 267, 549, 597, 1291, 1401, 1432, 1647
BmrFI CCNGG 8 cut(s) 348, 400, 417, 481, 945, 1364, 1713, 1828
BmsI GCATC 5 cut(s) 1002, 1312, 1405, 1494, 1670
BmtI GCTAGC 1 cut(s) 639
Bpu10I CCTNAGC 1 cut(s) 270
BpuEI CTTGAG 1 cut(s) 50
BpuMI CCSGG 1 cut(s) 481
Bsa29I ATCGAT 1 cut(s) 114
BsaBI GATNNNNATC 1 cut(s) 849
BsaJI CCNNGG 3 cut(s) 132, 346, 399
Bsc4I CCNNNNNNNGG 4 cut(s) 133, 368, 566, 944
Bse1I ACTGG 1 cut(s) 1192
Bse3DI GCAATG 2 cut(s) 905, 1455
Bse8I GATNNNNATC 1 cut(s) 849
BseBI CCWGG 7 cut(s) 348, 400, 417, 945, 1364, 1713, 1828
BseCI ATCGAT 1 cut(s) 114
BseDI CCNNGG 3 cut(s) 132, 346, 399
BseGI GGATG 3 cut(s) 850, 874, 1509
BseJI GATNNNNATC 1 cut(s) 849
BseLI CCNNNNNNNGG 4 cut(s) 133, 368, 566, 944
BseMI GCAATG 2 cut(s) 905, 1455
BseMII CTCAG 5 cut(s) 57, 261, 855, 1146, 1230
BseNI ACTGG 1 cut(s) 1192
BseSI GKGCMC 2 cut(s) 551, 717
BseXI GCAGC 4 cut(s) 471, 960, 1499, 1701
BseYI CCCAGC 1 cut(s) 424
BsgI GTGCAG 1 cut(s) 768
Bsh1236I CGCG 1 cut(s) 225
BshFI GGCC 3 cut(s) 549, 985, 1292
BshVI ATCGAT 1 cut(s) 114
BsiHKAI GWGCWC 2 cut(s) 276, 717
BsiHKCI CYCGRG 1 cut(s) 93
BsiSI CCGG 1 cut(s) 480
BslFI GGGAC 3 cut(s) 251, 457, 773
BslI CCNNNNNNNGG 4 cut(s) 133, 368, 566, 944
BsmFI GGGAC 3 cut(s) 251, 457, 773
BsmI GAATGC 1 cut(s) 1129
BsnI GGCC 3 cut(s) 549, 985, 1292
BsoBI CYCGRG 1 cut(s) 93
Bsp120I GGGCCC 1 cut(s) 547
Bsp1286I GDGCHC 3 cut(s) 276, 551, 717
Bsp1407I TGTACA 1 cut(s) 1258
Bsp143I GATC 9 cut(s) 595, 747, 796, 826, 844, 1242, 1399, 1645, 1759
BspACI CCGC 5 cut(s) 225, 332, 1123, 1213, 1591
BspANI GGCC 3 cut(s) 549, 985, 1292
BspCNI CTCAG 5 cut(s) 56, 262, 856, 1147, 1231
BspDI ATCGAT 1 cut(s) 114
BspFNI CGCG 1 cut(s) 225
BspLI GGNNCC 7 cut(s) 267, 549, 597, 1291, 1401, 1432, 1647
BspMI ACCTGC 1 cut(s) 370
BspOI GCTAGC 1 cut(s) 639
BspPI GGATC 7 cut(s) 590, 603, 852, 1394, 1407, 1640, 1653
BsrDI GCAATG 2 cut(s) 905, 1455
BsrGI TGTACA 1 cut(s) 1258
BsrI ACTGG 1 cut(s) 1192
BssECI CCNNGG 3 cut(s) 132, 346, 399
BssMI GATC 9 cut(s) 595, 747, 796, 826, 844, 1242, 1399, 1645, 1759
BssT1I CCWWGG 1 cut(s) 132
Bst2UI CCWGG 7 cut(s) 348, 400, 417, 945, 1364, 1713, 1828
Bst4CI ACNGT 6 cut(s) 247, 957, 1180, 1201, 1313, 1699
BstAPI GCANNNNNTGC 1 cut(s) 907
BstAUI TGTACA 1 cut(s) 1258
BstC8I GCNNGC 6 cut(s) 568, 637, 903, 1127, 1509, 1687
BstDEI CTNAG 6 cut(s) 43, 270, 864, 1155, 1239, 1375
BstENI CCTNNNNNAGG 1 cut(s) 366
BstF5I GGATG 3 cut(s) 850, 874, 1509
BstFNI CGCG 1 cut(s) 225
BstHHI GCGC 1 cut(s) 225
BstKTI GATC 9 cut(s) 598, 750, 799, 829, 847, 1245, 1402, 1648, 1762
BstMBI GATC 9 cut(s) 595, 747, 796, 826, 844, 1242, 1399, 1645, 1759
BstMWI GCNNNNNNNGC 5 cut(s) 20, 434, 476, 907, 1322
BstNI CCWGG 7 cut(s) 348, 400, 417, 945, 1364, 1713, 1828
BstNSI RCATGY 2 cut(s) 791, 1265
BstSCI CCNGG 8 cut(s) 346, 398, 415, 479, 943, 1362, 1711, 1826
BstSFI CTRYAG 2 cut(s) 1059, 1670
BstSLI GKGCMC 2 cut(s) 551, 717
BstUI CGCG 1 cut(s) 225
BstV1I GCAGC 4 cut(s) 471, 960, 1499, 1701
BstX2I RGATCY 4 cut(s) 595, 1242, 1399, 1645
BstXI CCANNNNNNTGG 2 cut(s) 439, 668
BstYI RGATCY 4 cut(s) 595, 1242, 1399, 1645
Bsu15I ATCGAT 1 cut(s) 114
BsuRI GGCC 3 cut(s) 549, 985, 1292
BsuTUI ATCGAT 1 cut(s) 114
BtgZI GCGATG 2 cut(s) 418, 597
BtrI CACGTC 1 cut(s) 712
BtsCI GGATG 3 cut(s) 850, 874, 1509
BtsIMutI CAGTG 6 cut(s) 462, 861, 953, 1080, 1210, 1318
BveI ACCTGC 1 cut(s) 370
Cac8I GCNNGC 6 cut(s) 568, 637, 903, 1127, 1509, 1687
CaiI CAGNNNCTG 1 cut(s) 50
CfoI GCGC 1 cut(s) 225
Cfr13I GGNCC 4 cut(s) 265, 547, 548, 1290
ClaI ATCGAT 1 cut(s) 114
CsiI ACCWGGT 2 cut(s) 1362, 1711
Csp6I GTAC 2 cut(s) 1259, 1453
CspCI CAANNNNNGTGG 2 cut(s) 1315, 1350
CviAII CATG 5 cut(s) 173, 289, 788, 1262, 1727
CviQI GTAC 2 cut(s) 1259, 1453
DdeI CTNAG 6 cut(s) 43, 270, 864, 1155, 1239, 1375
DpnI GATC 9 cut(s) 597, 749, 798, 828, 846, 1244, 1401, 1647, 1761
DpnII GATC 9 cut(s) 595, 747, 796, 826, 844, 1242, 1399, 1645, 1759
DraI TTTAAA 1 cut(s) 501
DraIII CACNNNGTG 2 cut(s) 957, 1522
EciI GGCGGA 1 cut(s) 347
Ecl136II GAGCTC 1 cut(s) 274
Eco130I CCWWGG 1 cut(s) 132
Eco147I AGGCCT 1 cut(s) 985
Eco24I GRGCYC 2 cut(s) 276, 551
Eco32I GATATC 2 cut(s) 112, 144
Eco47I GGWCC 1 cut(s) 265
Eco53kI GAGCTC 1 cut(s) 274
Eco57I CTGAAG 8 cut(s) 387, 486, 666, 720, 821, 972, 1302, 1671
Eco88I CYCGRG 1 cut(s) 93
EcoICRI GAGCTC 1 cut(s) 274
EcoNI CCTNNNNNAGG 1 cut(s) 366
EcoO109I RGGNCCY 3 cut(s) 265, 547, 548
EcoRII CCWGG 7 cut(s) 346, 398, 415, 943, 1362, 1711, 1826
EcoRV GATATC 2 cut(s) 112, 144
EcoT14I CCWWGG 1 cut(s) 132
EcoT38I GRGCYC 2 cut(s) 276, 551
ErhI CCWWGG 1 cut(s) 132
FaeI CATG 5 cut(s) 176, 292, 791, 1265, 1730
FaqI GGGAC 3 cut(s) 251, 457, 773
FatI CATG 5 cut(s) 172, 288, 787, 1261, 1726
FauNDI CATATG 2 cut(s) 1345, 1773
FbaI TGATCA 1 cut(s) 826
FblI GTMKAC 1 cut(s) 373
Fnu4HI GCNGC 6 cut(s) 226, 460, 949, 1488, 1592, 1690
FokI GGATG 3 cut(s) 837, 881, 1516
FriOI GRGCYC 2 cut(s) 276, 551
Fsp4HI GCNGC 6 cut(s) 226, 460, 949, 1488, 1592, 1690
FspBI CTAG 3 cut(s) 636, 726, 1277
GlaI GCGC 1 cut(s) 224
GluI GCNGC 6 cut(s) 226, 460, 949, 1488, 1592, 1690
GsaI CCCAGC 1 cut(s) 428
HaeIII GGCC 3 cut(s) 549, 985, 1292
HapII CCGG 1 cut(s) 480
HhaI GCGC 1 cut(s) 225
Hin1II CATG 5 cut(s) 176, 292, 791, 1265, 1730
Hin6I GCGC 1 cut(s) 223
HinP1I GCGC 1 cut(s) 223
HincII GTYRAC 1 cut(s) 1120
HindII GTYRAC 1 cut(s) 1120
HindIII AAGCTT 2 cut(s) 1304, 1653
HpaI GTTAAC 1 cut(s) 1120
HpaII CCGG 1 cut(s) 480
HphI GGTGA 2 cut(s) 314, 806
Hpy166II GTNNAC 7 cut(s) 374, 709, 715, 1120, 1259, 1341, 1498
Hpy8I GTNNAC 7 cut(s) 374, 709, 715, 1120, 1259, 1341, 1498
HpyCH4III ACNGT 6 cut(s) 247, 957, 1180, 1201, 1313, 1699
HpyCH4IV ACGT 3 cut(s) 711, 969, 1518
HpyF10VI GCNNNNNNNGC 5 cut(s) 20, 434, 476, 907, 1322
HpyF3I CTNAG 6 cut(s) 43, 270, 864, 1155, 1239, 1375
HpySE526I ACGT 3 cut(s) 711, 969, 1518
Hsp92II CATG 5 cut(s) 176, 292, 791, 1265, 1730
HspAI GCGC 1 cut(s) 223
Ksp22I TGATCA 1 cut(s) 826
KspAI GTTAAC 1 cut(s) 1120
Kzo9I GATC 9 cut(s) 595, 747, 796, 826, 844, 1242, 1399, 1645, 1759
LmnI GCTCC 3 cut(s) 433, 1430, 1484
Lsp1109I GCAGC 4 cut(s) 471, 960, 1499, 1701
LweI GCATC 5 cut(s) 1002, 1312, 1405, 1494, 1670
MabI ACCWGGT 2 cut(s) 1362, 1711
MaeI CTAG 3 cut(s) 636, 726, 1277
MaeII ACGT 3 cut(s) 711, 969, 1518
MaeIII GTNAC 8 cut(s) 27, 521, 805, 1041, 1215, 1522, 1598, 1609
MalI GATC 9 cut(s) 597, 749, 798, 828, 846, 1244, 1401, 1647, 1761
MboI GATC 9 cut(s) 595, 747, 796, 826, 844, 1242, 1399, 1645, 1759
MboII GAAGA 5 cut(s) 70, 590, 1047, 1094, 1687
MflI RGATCY 4 cut(s) 595, 1242, 1399, 1645
MhlI GDGCHC 3 cut(s) 276, 551, 717
MluCI AATT 6 cut(s) 86, 618, 687, 1028, 1464, 1616
MlyI GAGTC 4 cut(s) 33, 98, 1109, 1547
MmeI TCCRAC 2 cut(s) 126, 1377
MseI TTAA 6 cut(s) 473, 500, 921, 1119, 1149, 1368
MslI CAYNNNNRTG 5 cut(s) 177, 437, 792, 1391, 1697
MspI CCGG 1 cut(s) 480
MspR9I CCNGG 8 cut(s) 348, 400, 417, 481, 945, 1364, 1713, 1828
Mva1269I GAATGC 1 cut(s) 1129
MvaI CCWGG 7 cut(s) 348, 400, 417, 945, 1364, 1713, 1828
MvnI CGCG 1 cut(s) 225
MwoI GCNNNNNNNGC 5 cut(s) 20, 434, 476, 907, 1322
NciI CCSGG 1 cut(s) 481
NdeI CATATG 2 cut(s) 1345, 1773
NdeII GATC 9 cut(s) 595, 747, 796, 826, 844, 1242, 1399, 1645, 1759
NheI GCTAGC 1 cut(s) 635
NlaIII CATG 5 cut(s) 176, 292, 791, 1265, 1730
NlaIV GGNNCC 7 cut(s) 267, 549, 597, 1291, 1401, 1432, 1647
NmuCI GTSAC 5 cut(s) 27, 521, 1215, 1522, 1609
NspI RCATGY 2 cut(s) 791, 1265
OliI CACNNNNGTG 1 cut(s) 1697
PceI AGGCCT 1 cut(s) 985
PciI ACATGT 2 cut(s) 787, 1261
PctI GAATGC 1 cut(s) 1129
PfeI GAWTC 6 cut(s) 73, 116, 536, 626, 1631, 1791
PfoI TCCNGGA 1 cut(s) 415
PkrI GCNGC 6 cut(s) 227, 461, 950, 1489, 1593, 1691
PleI GAGTC 4 cut(s) 33, 98, 1108, 1546
PpsI GAGTC 4 cut(s) 33, 98, 1108, 1546
PpuMI RGGWCCY 1 cut(s) 265
PscI ACATGT 2 cut(s) 787, 1261
Psp124BI GAGCTC 1 cut(s) 276
Psp5II RGGWCCY 1 cut(s) 265
Psp6I CCWGG 7 cut(s) 346, 398, 415, 943, 1362, 1711, 1826
PspFI CCCAGC 1 cut(s) 424
PspGI CCWGG 7 cut(s) 346, 398, 415, 943, 1362, 1711, 1826
PspN4I GGNNCC 7 cut(s) 267, 549, 597, 1291, 1401, 1432, 1647
PspOMI GGGCCC 1 cut(s) 547
PspPI GGNCC 4 cut(s) 265, 547, 548, 1290
PspPPI RGGWCCY 1 cut(s) 265
PstNI CAGNNNCTG 1 cut(s) 50
PsuI RGATCY 4 cut(s) 595, 1242, 1399, 1645
RsaI GTAC 2 cut(s) 1260, 1454
RsaNI GTAC 2 cut(s) 1259, 1453
RseI CAYNNNNRTG 5 cut(s) 177, 437, 792, 1391, 1697
SacI GAGCTC 1 cut(s) 276
SaqAI TTAA 6 cut(s) 473, 500, 921, 1119, 1149, 1368
SatI GCNGC 6 cut(s) 226, 460, 949, 1488, 1592, 1690
Sau3AI GATC 9 cut(s) 595, 747, 796, 826, 844, 1242, 1399, 1645, 1759
Sau96I GGNCC 4 cut(s) 265, 547, 548, 1290
SchI GAGTC 4 cut(s) 33, 98, 1109, 1547
ScrFI CCNGG 8 cut(s) 348, 400, 417, 481, 945, 1364, 1713, 1828
SduI GDGCHC 3 cut(s) 276, 551, 717
SexAI ACCWGGT 2 cut(s) 1362, 1711
SfaNI GCATC 5 cut(s) 1002, 1312, 1405, 1494, 1670
SfcI CTRYAG 2 cut(s) 1059, 1670
SinI GGWCC 1 cut(s) 265
SmiMI CAYNNNNRTG 5 cut(s) 177, 437, 792, 1391, 1697
SmlI CTYRAG 1 cut(s) 65
SmoI CTYRAG 1 cut(s) 65
Sse9I AATT 6 cut(s) 86, 618, 687, 1028, 1464, 1616
SseBI AGGCCT 1 cut(s) 985
SsiI CCGC 5 cut(s) 225, 332, 1123, 1213, 1591
SspI AATATT 1 cut(s) 1407
SspMI CTAG 3 cut(s) 636, 726, 1277
SstI GAGCTC 1 cut(s) 276
StuI AGGCCT 1 cut(s) 985
StyD4I CCNGG 8 cut(s) 346, 398, 415, 479, 943, 1362, 1711, 1826
StyI CCWWGG 1 cut(s) 132
TaaI ACNGT 6 cut(s) 247, 957, 1180, 1201, 1313, 1699
TaiI ACGT 3 cut(s) 714, 972, 1521
TaqI TCGA 3 cut(s) 114, 750, 1002
TasI AATT 6 cut(s) 86, 618, 687, 1028, 1464, 1616
TatI WGTACW 1 cut(s) 1258
TauI GCSGC 2 cut(s) 228, 1594
TfiI GAWTC 6 cut(s) 73, 116, 536, 626, 1631, 1791
Tru1I TTAA 6 cut(s) 473, 500, 921, 1119, 1149, 1368
Tru9I TTAA 6 cut(s) 473, 500, 921, 1119, 1149, 1368
TscAI CASTG 6 cut(s) 469, 868, 960, 1080, 1210, 1318
TseFI GTSAC 5 cut(s) 27, 521, 1215, 1522, 1609
TseI GCWGC 4 cut(s) 459, 948, 1487, 1689
Tsp45I GTSAC 5 cut(s) 27, 521, 1215, 1522, 1609
TspDTI ATGAA 5 cut(s) 161, 277, 591, 693, 1457
TspRI CASTG 6 cut(s) 469, 868, 960, 1080, 1210, 1318
VneI GTGCAC 1 cut(s) 713
VpaK11BI GGWCC 1 cut(s) 265
XagI CCTNNNNNAGG 1 cut(s) 366
XapI RAATTY 3 cut(s) 86, 618, 1464
XceI RCATGY 2 cut(s) 791, 1265
XcmI CCANNNNNNNNNTGG 2 cut(s) 868, 1393
XmiI GTMKAC 1 cut(s) 373
XspI CTAG 3 cut(s) 636, 726, 1277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.